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Free Radical Research

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https://www.readbyqxmd.com/read/28301979/short-time-uva-exposure-to-human-keratinocytes-instigated-polyunsaturated-fatty-acid-without-inducing-lipid-peroxidation
#1
Kin Sum Leung, Hok Fung Chan, Ho Hang Leung, Jean-Marie Galano, Camille Oger, Thierry Durand, Jetty Chung-Yung Lee
Short-term exposure to ultraviolet A (UVA) radiation can directly injure our skin through inflammatory response and indirectly through oxidative stress, triggering polyunsaturated fatty acid (PUFA) peroxidation in skin cell membrane and formation of DNA adduct, 8-hydroxy-2'-deoxyguanosine (8-OHdG). It is known that UVA exposure leads to photoaging, immunosuppression and skin cancer. However, the changes in PUFA and its oxidized metabolites, and cell cycle after short UVA exposure, are debatable. In this study, human keratinocytes (HaCaT) were exposed to low dose (5 J/cm(2)) and high dose (20 J/cm(2)) of UVA and assessed immediately, 8 h, 12 h, and 24 h post-treatment...
March 17, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28301981/alterations-of-hepatocyte-function-with-free-radical-generators-and-reparation-or-prevention-with-coffee-polyphenols
#2
Amel Saidi Merzouk, Merzouk Hafida, Amel Medjdoub, Bouchra Loukidi, Sabri Cherrak, Sid Ahmed Merzouk, Mourad Elhabiri
Liver diseases are linked in the majority of cases to oxidative stress that antioxidants could neutralize with reducing liver injury. Chlorogenic acid, a coffee polyphenol, possesses antioxidant prosperities. The aim of this study was to evaluate in vitro preventive and corrective effects of cholorogenic acid in hepatocyte toxicity induced by free radicals. Hepatocytes were isolated from adult male Wistar rats. To determine corrective effects and reparation, cells were first exposed to two free radical generators (Hydrogen peroxide/Iron sulfate for hydroxyl radical formation, and Phenazine methosulfate/Nicotinamide adenine dinucleotide for superoxide anion formation) for 12H and thereafter treated by chlorogenic acid (1 and 10 µM final concentration) for another 12H...
March 16, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28277985/indoxyl-sulfate-potentiates-endothelial-dysfunction-via-reciprocal-role-for-reactive-oxygen-species-and-rhoa-rock-signaling-in-5-6-nephrectomized-rats
#3
Shuang Chu, Xiaodong Mao, Hengjiang Guo, Li Wang, Zezheng Li, Yang Zhang, Yunman Wang, Hao Wang, Xuemei Zhang, Wen Peng
Accumulative indoxyl sulfate (IS) retained in chronic kidney disease (CKD) can potentiate vascular endothelial dysfunction, and herein, we aim at elucidating the underlying mechanisms from the perspective of possible association between reactive oxygen species (ROS) and RhoA/ROCK pathway. IS-treated nephrectomized rats are administered with antioxidants including NADPH oxidase inhibitor apocynin, SOD analog tempol, and mitochondrion-targeted SOD mimetic mito-TEMPO to scavenge ROS, or ROCK inhibitor fasudil to obstruct RhoA/ROCK pathway...
March 13, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28277984/neutral-sphingomyelinase-inhibition-alleviates-apoptosis-but-not-er-stress-in-liver-ischemia-reperfusion-injury
#4
Hazal Tuzcu, Betul Unal, Ebru Kırac, Esma Konuk, Filiz Ozcan, Gulsum O Elpek, Necdet Demir, Mutay Aslan
Previous studies have revealed the activation of neutral sphingomyelinase (N-SMase)/ceramide pathway in hepatic tissue following warm liver ischemia reperfusion (IR) injury. Excessive ceramide accumulation is known to potentiate apoptotic stimuli and a link between apoptosis and endoplasmic reticulum (ER) stress has been established in hepatic IR injury. Thus, this study determined the role of selective N-SMase inhibition on ER stress and apoptotic markers in a rat model of liver IR injury. Selective N-SMase inhibitor was administered via intraperitoneal injections...
March 13, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28277983/l-arginine-supplementation-improves-rats-antioxidant-system-and-exercise-performance
#5
E P Silva, L S Borges, C Mendes-da-Silva, S M Hirabara, R H Lambertucci
Reactive species have great importance in sports performance, once they can directly regulate energy production, muscular contraction, inflammation and fatigue. Therefore, the redox control is essential for athletes' performance. Studies demonstrated that L-arginine has an important role in the synthesis of urea, cell growth and production of nitric oxide, moreover, there are indications that it is also able to induce benefits to muscle antioxidant system through the upregulation of some antioxidant enzymes, and by inhibiting some pathways of reactive species production...
March 1, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28287049/impact-of-processing-conditions-on-the-phytoprostanes-profile-of-three-types-of-nut-kernels
#6
Ana María Carrasco-Del Amor, Encarna Aguayo, Jacinta Collado-González, Alexandre Guy, Jean-Marie Galano, Thierry Durand, Ángel Gil-Izquierdo
The goal of this work was the identification and quantification of phytoprostanes (PhytoPs) in three types of nuts: "Walnut", "Macadamia", and "Pecan". This study represents a first approach to the relationship between the quantitative and qualitative PhytoP profiles in the "Macadamia" and "Pecan" nuts subjected to fried salt or fried honey processing. The kernels were found to contain 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, 16-B1-PhytoP, ent-16-B1-PhytoP, 9-L1-PhytoP, and ent-9-L1-PhytoP. "Macadamia" fried salt nuts were the only ones that produced 9-epi-9-D1-PhytoP and 9-D1-PhytoP...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28287048/lycium-barbarum-polysaccharide-protects-human-keratinocytes-against-uvb-induced-photo-damage
#7
Huaping Li, Zhenjie Li, Liqian Peng, Na Jiang, Qing Liu, Erting Zhang, Bihua Liang, Runxiang Li, Huilan Zhu
Ultraviolet B (UVB) irradiation plays a key role in skin damage, which induces oxidative and inflammatory damages, thereby causing photoaging or photocarcinogenesis. Lycium barbarum polysaccharide (LBP), the most biologically active fraction of wolfberry, possesses significant antioxidative and anti-inflammatory effects on multiple tissues. In the present study, the photoprotective effects and potential underlying molecular mechanisms of LBP against UVB-induced photo-damage were investigated in immortalized human keratinocytes (HaCaT cells)...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28277986/bardoxolone-methyl-inhibits-migration-and-metabolism-in-mcf7-cells
#8
Alaa Refaat, Chathyan Pararasa, Muhammed Arif, James E P Brown, Amtul Carmichael, Sameh S Ali, Hiroaki Sakurai, Helen R Griffiths
Bardoxolone-methyl (BAR) is reported to have anti-inflammatory, anti-proliferative and anti-fibrotic effects. BAR activates Nrf2 and may ameliorate oxidative stress through induction of antioxidant genes. However, off-target effects, probably concentration and NFkB-dependent, have limited the clinical use of BAR. Nrf2 regulates expression of antioxidant and mitochondrial genes and has been proposed as a target for both obesity and breast cancer. Therefore, we explored whether BAR can alter migration and proliferation in the MCF7 cell line and whether metabolic function is affected by BAR...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28166653/oxidative-stress-and-inflammation-liver-responses-and-adaptations-to-acute-and-regular-exercise
#9
Rômulo Pillon Barcelos, Luiz Fernando Freire Royes, Javier Gonzalez-Gallego, Guilherme Bresciani
The liver is remarkably important during exercise outcomes due to its contribution to detoxification, synthesis, and release of biomolecules, and energy supply to the exercising muscles. Recently, liver has been also shown to play an important role in redox status and inflammatory modulation during exercise. However, while several studies have described the adaptations of skeletal muscles to acute and chronic exercise, hepatic changes are still scarcely investigated. Indeed, acute intense exercise challenges the liver with increased reactive oxygen species (ROS) and inflammation onset, whereas regular training induces hepatic antioxidant and anti-inflammatory improvements...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28166650/the-effects-of-exercise-load-during-development-on-oxidative-stress-levels-and-antioxidant-potential-in-adulthood
#10
S Lee, J Hashimoto, T Suzuki, A Satoh
The objective of this study was to elucidate the impact of physical activity during the growth period as well as on oxidative stress and antioxidative potential in adulthood. The experimental animals used were four-week old male Wistar rats, which were randomly divided into three groups. The exercise loads were as follows: control (CON), treadmill exercise (TE), and jumping exercise (JE). The exercise was performed at the same time of day, at a frequency of five days per week, for eight weeks. Derivatives of reactive oxygen metabolites (d-ROSs) and biological antioxidant potential (BAP) were measured during periods of rest prior to commencement of the experiment and after the experiment...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28166649/evaluation-of-plasma-antioxidant-activity-in-rats-given-excess-egcg-with-reference-to-endogenous-antioxidants-concentrations-and-assay-methods
#11
Kaori Yokotani, Keizo Umegaki
The contribution of (-)-epigallocatechin gallate (EGCg) intake to in vivo antioxidant activity is unclear, even with respect to plasma. In this study, we examined how administration of EGCg contributes to plasma antioxidant activity, relative to its concentration, endogenous antioxidants, and assay methods, namely oxygen radical absorbance capacity (ORAC) and ferric reducing/antioxidant power (FRAP). Administration of EGCg (500 mg/kg) to rats increased plasma EGCg (4μmol/L as free form) and ascorbic acid (1...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28166645/epr-imaging-and-hplc-characterization-of-the-pigment-based-organic-free-radical-in-black-soybean-seeds
#12
Kouichi Nakagawa, Hayato Maeda
We investigated the location and distribution of paramagnetic species in dry black, brown, and yellow (normal) soybean seeds using electron paramagnetic resonance (EPR), X-band (9 GHz) EPR imaging (EPRI), and HPLC. EPR primarily detected two paramagnetic species in black soybean. These two different radical species were assigned as stable organic radical and Mn(2+ )species based on the g values and hyperfine structures. The signal from the stable radical was noted at g ≈ 2.00 and was relatively strong and stable...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28147890/astragaloside-iv-synergizes-with-ferulic-acid-to-suppress-hepatic-stellate-cells-activation-in-vitro
#13
Haiying Dong, Hongyan Guo, Yini Liang, Xing Wang, Yingcai Niu
Because hepatic fibrosis usually involves more than one pathological process, combination therapy with modalities that target aberrant signaling cascade in activated hepatic stellate cells (HSCs) represents an alternative strategy. This study evaluates the hypothesis that astragaloside IV (AS-IV) and ferulic acid (FA) synergize to inhibit HSCs activation via simultaneous activating nuclear factor erythroid-2-related factor-2 (Nrf2) and blocking transforming growth factor-β (TGF-β) pathways. The combination of FA and AS-IV, hereafter referred to as the AS-IV/FA, at suboptimal concentrations synergistically inhibited HSCs activation, as measured by expressions of α-smooth muscle actin (α-SMA), collagen α type I (Col I) and fibronectin...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28142292/the-role-of-peroxiredoxin-6-in-neutralization-of-x-ray-mediated-oxidative-stress-effects-on-gene-expression-preservation-of-radiosensitive-tissues-and-postradiation-survival-of-animals
#14
M G Sharapov, V I Novoselov, E E Fesenko, V I Bruskov, S V Gudkov
Peroxiredoxins are redox-sensing multifunctional enzymes, among them peroxiredoxin 6 (Prx6) can neutralize the most broadest range of hydroperoxides and play an important role in maintaining the redox homeostasis of the cell. In the present study, radioprotective and signaling regulatory effects of Prx6 were demonstrated and characterized. Intravenously administered exogenous Prx6 protects the organism of mice from the destructive action of ionizing radiation in the lethal dose range of 5-10 Gy. Dose reduction factor of 1...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28118761/on-the-complex-%C3%A2-oh-%C3%A2-o-induced-free-radical-chemistry-of-arylalkylamines-with-special-emphasis-on-the-contribution-of-the-alkylamine-side-chain
#15
László Szabó, Viktória Mile, Tünde Tóth, György T Balogh, Tamás Földes, Erzsébet Takács, László Wojnárovits
A full account of the (•)OH-induced free radical chemistry of an arylalkylamine is given taking all the possible reaction pathways quantitatively into consideration. Such knowledge is indispensable when the alkylamine side chain plays a crucial role in biological activity. The fundamental reactions are investigated on the model compound N-methyl-3-phenypropylamine (MPPA), and extended to its biologically active analog, to the antidepressant fluoxetine (FLX). Pulse radiolysis techniques were applied including redox titration and transient spectral analysis supplemented with DFT calculations...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28112004/torulene-and-torularhodin-protects-human-prostate-stromal-cells-from-hydrogen-peroxide-induced-oxidative-stress-damage-through-the-regulation-of-bcl-2-bax-mediated-apoptosis
#16
Chao Du, Yahui Guo, Yuliang Cheng, Mei Han, Weiguo Zhang, He Qian
The current study was designed to elucidate the cytoprotective effects and possible mechanisms of torulene and torularhodin on hydrogen peroxide (H2O2)-induced oxidative stress damage in human prostate stromal cells (WPMY-1). After treated with H2O2, a notable decrease was appeared in cell viability, yet the decrease was attenuated when cells were pretreated with torulene and torularhodin (0.5-10 μM) as evaluated by WST-1 assay. Pretreatment with these two carotenoids significantly attenuated H2O2-induced apoptosis in WPMY-1 cells through the inhibition of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) overproduction, as well as the activation of the activities in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px)...
February 2017: Free Radical Research
https://www.readbyqxmd.com/read/28095729/impact-of-reactive-oxygen-species-generation-on-helicobacter-pylori-related-extragastric-diseases-a-hypothesis
#17
Jannis Kountouras, Marina Boziki, Stergios A Polyzos, Panagiotis Katsinelos, Emmanouel Gavalas, Christos Zeglinas, Dimitri Tzivras, Iordanis Romiopoulos, Nikolaos Giorgakis, Kyriaki Anastasiadou, Elizabeth Vardaka, Constantinos Kountouras, Evangelos Kazakos, Georgia Xiromerisiou, Efthimios Dardiotis, Georgia Deretzi
Helicobacter pylori (H. pylori) induces reactive oxygen species (ROS) production that contribute to pathogenesis of a variety of H. pylori-related gastric diseases, as shown in animal and human studies. Helicobacter pylori infection is also associated with variety of systemic extragastric diseases in which H. pylori-related ROS production might also be involved in the pathogenesis of these systemic conditions. We proposed that Hp-related ROS may play a crucial role in the pathophysiology of Hp-related systemic diseases including Alzheimer's disease, multiple sclerosis, glaucoma and other relative neurodegenerative diseases, thereby suggesting introduction of relative ROS scavengers as therapeutic strategies against these diseases which are among the leading causes of disability and are associated with a large public health global burden...
January 2017: Free Radical Research
https://www.readbyqxmd.com/read/28090788/selection-of-suitable-reference-genes-for-quantitative-real-time-pcr-in-trabecular-meshwork-cells-under-oxidative-stress
#18
Jing Zhao, HongYan Zhou, Lixia Sun, Ben Yang, Lin Zhang, Hongfeng Shi, Yajuan Zheng
Oxidative stress-induced dysfunction in trabecular meshwork (TM) cells is considered a major alteration that can lead to glaucoma. Hydrogen peroxide (H2O2) is the most widely used agent for inducing oxidation in TM cells in vitro. Quantitative real-time PCR (qPCR) is an important method for studying alterations in gene expression, and suitable (i.e. invariant) reference genes must be defined to normalize expression levels. In this study, eight common reference genes, i.e. PRS18, ACTB, B2M, GAPDH, PPIA, HPRT1, YWHAZ, and TBP, were evaluated for use in studies of H2O2-induced dysfunction in TM cells...
January 2017: Free Radical Research
https://www.readbyqxmd.com/read/28090786/inorganic-nitrite-modulates-mirna-signatures-in-acute-myocardial-in-vivo-ischemia-reperfusion
#19
Ulrike B Hendgen-Cotta, Daniel Messiha, Sonja Esfeld, René Deenen, Tienush Rassaf, Matthias Totzeck
Acute myocardial infarction is the leading cause of mortality in the industrialized world. While it is essential to attempt an early reperfusion of ischemic myocardial territories, reperfusion itself adds damage to the heart, the ischemia-reperfusion (I/R) injury. Particularly the injury resulting from the very first minutes of reperfusion remains incompletely understood. MicroRNAs (miRNAs) are dynamic regulators in I/R injury. Nitric oxide (•NO) signaling, in turn, interacts with miRNA signaling. Our previous investigations showed that •NO signaling in I/R could be modulated by nitrite...
January 2017: Free Radical Research
https://www.readbyqxmd.com/read/28081640/xct-deficiency-aggravates-acetaminophen-induced-hepatotoxicity-under-inhibition-of-the-transsulfuration-pathway
#20
Eun Sil Kang, Jaeyong Lee, Takujiro Homma, Toshihiro Kurahashi, Sho Kobayashi, Atsunori Nabeshima, Sohsuke Yamada, Han Geuk Seo, Satoshi Miyata, Hideyo Sato, Junichi Fujii
Cystine, an oxidized form of cysteine (Cys), is imported into cells via the protein xCT, which is also associated with the export of glutamate as the counter amino acid. In the current study, we attempted to rationalize roles of xCT in the livers of male mice. While xCT was not expressed in the livers of ordinary mice, it was induced under conditions of glutathione depletion, caused by the administration of acetaminophen (AAP). To differentiate the role between xCT and the transsulfuration pathway on the supply of Cys, we employed an inhibitor of the enzyme cystathionine γ-lyase, propargylglycine (PPG)...
January 2017: Free Radical Research
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