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Free Radical Research

Ji-Hong Hu, Jing-Jing Nie, Zhen-Xiang Gao, Qing-Hua Weng, Zhen-He Wang, Chuan-Bao Li, Ya-Ya Pian, Ran Zhang, Zhe-Li Jiang, Meng-Ming Xia, Jian-Ping Cai
Emerging evidence suggests that microbial pathogens may induce oxidative stress in infected hosts. The aim of the present study was to investigate the relationship between changes in oxidative stress and intestinal infection with and without antibiotic treatment in animal models. Sprague-Dawley (SD) rats were divided into three groups: rats infected with Salmonella enterica serovar Enteritidis (S. enteritidis), rats infected with S. enteritidis followed by norfloxacin treatment, and the control group. To evaluate oxidative stress changes, levels of 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dGsn), which represented oxidative damage to RNA and DNA, respectively, were analysed in urine and tissue samples...
November 13, 2018: Free Radical Research
Geetha Mathew, Arpeeta Sharma, Raelene J Pickering, Carlos J Rosado, Jeremie Lemarie, Jayesh Mudgal, Magith Thambi, Sarine Sebastian, Karin A Jandeleit-Dahm, Judy B de Haan, Mazhuvancherry K Unnikrishnan
Inflammation is a protective immune response against invading pathogens, however, dysregulated inflammation is detrimental. As the complex inflammatory response involves multiple mediators, including the involvement of reactive oxygen species, concomitantly targeting proinflammatory and antioxidant check-points may be a more rational strategy. We report the synthesis and anti-inflammatory/antioxidant activity of a novel indanedione derivative DMFO. DMFO scavenged reactive oxygen species (ROS) in in-vitro radical scavenging assays and in lipopolysaccharide (LPS)-stimulated RAW 264...
November 13, 2018: Free Radical Research
Suhyun Kim, Dong Gun Lee
PMAP-23 ( RIIDLLWRVRRPQKPKFVTVWVR -NH2 ) is an antimicrobial peptide (AMP) derived from porcine myeloid. Membrane disruption is thought to underpin the anticandidal activity of PMAP-23. However, many AMPs act via mechanisms other than simple membrane permeabilization. Here, we investigated the anticandidal mechanism of PMAP-23 at low concentrations. Membrane disruption and depolarization and rapid K+ efflux were observed in Candida albicans cells treated with 5 µM PMAP-23. In contrast, 2.5 µM PMAP-23 caused membrane depolarization and K+ efflux without membrane disruption...
November 7, 2018: Free Radical Research
Łukasz Pułaski, Izabela Jatczak-Pawlik, Marta Sobalska-Kwapis, Dominik Strapagiel, Grzegorz Bartosz, Izabela Sadowska-Bartosz
An alkylating compound, 3-bromopyruvic acid (3-3-bromopyruvic acid (BP)) is a promising anticancer agent, potentially able to act on multidrug-resistant cells. Its action has been attributed mainly to inhibition of glycolysis. This compound induces also oxidative stress at a cellular level. The effects of 3-BP on gene expression have not been studied although they may determine the survival of cells exposed to 3-BP. The aim of this paper was to examine the effect 3-BP on gene expression pattern in breast MCF-7 cancer cells...
October 26, 2018: Free Radical Research
Samantha Scheinok, Philippe Leveque, Pierre Sonveaux, Benoit Driesschaert, Bernard Gallez
As superoxide anion is of keen interest in biomedical research, it is highly desirable to have a technique allowing its detection sensitively and specifically in biological media. If electron paramagnetic resonance (EPR) techniques and probes have been individually described in the literature, there is actually no comparison of these techniques in the same conditions that may help guiding researchers for selecting the most appropriate approach. The aim of the present study was to compare different EPR strategies in terms of sensitivity and specificity to detect superoxide (vs...
October 26, 2018: Free Radical Research
Anna Laporte, Anke Nordenbrock, Sigurd Lenzen, Matthias Elsner
Hydrogen peroxide plays an important role in various biological processes in numerous organisms. Depending on the concentration and the distribution within the cell, it can act as stressor or redox signalling molecule. To analyse the effects of H2 O2 and its diffusion within the cell we developed the new genetically encoded photosensitizer KillerRed-SOD1 which enables a light-induced spatially and temporally controlled generation of H2 O2 in living cells. The KillerRed-SOD1 is a fusion protein of the photosensitizer KillerRed (KR) and the cytosolic superoxide dismutase isoform 1 (SOD1) connected by a helix-forming peptide linker...
October 23, 2018: Free Radical Research
Xi Liu, Wei Cao, Jia Qi, Qing Li, Min Zhao, Zhuyun Chen, Jingfeng Zhu, Zhimin Huang, Lin Wu, Bo Zhang, Yanggang Yuan, Changying Xing
Leonurine, a major bioactive component from Herba Leonuri, shows therapeutic potential in several diseases, including diabetes, cardiovascular disease, bovine mastitis and depression. In kidney, it was reported that leonurine was performing a protective effect in both acute kidney injury and renal fibrosis mice models. The aim of this study is to investigate the effect of leonurine in podocyte injury. In the mice model of adriamycin (ADR) -induced nephropathy, the application of leonurine significantly prevented early kidney damage, macrophage infiltration and proteinuria...
October 18, 2018: Free Radical Research
Bożena Adamczyk, Dorota Koziarska, Sławomir Kasperczyk, Monika Adamczyk-Sowa
So far little has been known about antioxidant properties of immunomodulatory drugs. The aim of the study was to evaluate the antioxidant status in serum of relapsing-remitting multiple sclerosis (RRMS) patients treated with II-line immunomodulatory therapy compared to de novo diagnosed patients, subjects treated with interferon (IFN) beta and healthy controls. We analyzed the relationships depending on the gender, age, disease duration, the Expanded Disability Status Scale (EDSS), the Annualized Relapse Rate (ARR) and MRI lesions in patients treated with II-line...
October 16, 2018: Free Radical Research
Satomi Funahashi, Yasumasa Okazaki, Takahiro Nishiyama, Hidekazu Ohyoshi, Hiroyuki Yasui, Kazuki Nishida, Shigeyuki Matsui, Shinya Toyokuni
Exposure to asbestos fiber is central to mesothelial carcinogenesis, for which iron overload in or near mesothelial cells is a key pathogenic mechanism. Alternatively, iron chelation therapy with deferasirox or regular phlebotomy was significantly preventive against crocidolite-induced mesothelial carcinogenesis in rats. However, the role of iron transporters during asbestos-induced carcinogenesis remains elusive. Here, we studied the role of divalent metal transporter 1 (DMT1; Slc11a2), which is a Fe(II) transporter, that is present not only on the apical plasma membrane of duodenal cells but also on the lysosomal membrane of every cell, in crocidolite-induced mesothelial carcinogenesis using DMT1 transgenic (DMT1Tg) mice...
October 12, 2018: Free Radical Research
Tomasz Nowak, Paweł Niemiec, Tomasz Iwanicki, Anna Balcerzyk, Jolanta Krauze, Anna Ochalska-Tyka, Iwona Zak
BACKGROUND: The p22phox is a critical component of vascular NADPH oxidases and is encoded by the CYBA gene. It was shown that functionally relevant polymorphisms of the CYBA gene - 930A>G, -852C>G , -675A>T , -536C>T, 214C>T (previously described as 242C>T), *24A>G (previously described as 640A>G), and *49A>G modulate generation of reactive oxygen species (ROS). AIM: Analyse whether the CYBA gene polymorphisms - 852C>G, -675A>T , and - 536C>T were associated with coronary artery disease (CAD), and to designate haplotype blocks...
October 8, 2018: Free Radical Research
Ji Geng, Xiao Yuan, Mingzhen Wei, Junchao Wu, Zheng-Hong Qin
TP53-induced glycolysis and apoptosis regulator (TIGAR) is a p53 target protein that plays critical roles in glycolysis and redox balance. Accumulating evidence shows that TIGAR is highly expressed in cancer. TIGAR redirects glycolysis and promotes carcinoma growth by providing metabolic intermediates and reductive power derived from pentose phosphate pathway (PPP). The expression of TIGAR in cancer is positively associated with chemotherapy resistance, suggesting that TIGAR could be a novel therapeutic target...
October 4, 2018: Free Radical Research
Tom M Nolte, Willie J G M Peijnenburg
The reaction between superoxide (O2 ·- ) and organic chemicals is of interest in many scientific disciplines including biology and synthetic chemistry, as well as for the evaluation of chemical fate in the environment. Due to limited data and lack of congeneric modeling, the involvement of superoxide in many complex processes cannot be adequately evaluated. In this study, we developed new Quantitative Structure-Property Relationship (QSPR) models for the prediction of the aqueous-phase rate constant for the reaction between superoxide and a wide variety of organic chemicals reacting via one-electron oxidation, reduction and hydrogen-transfer...
October 1, 2018: Free Radical Research
Said H Audi, Nina Friedly, Ranjan K Dash, Andreas M Beyer, Anne V Clough, Elizabeth R Jacobs
The objectives of this study were to develop a robust protocol to measure the rate of hydrogen peroxide (H2 O2 ) production in isolated perfused rat lungs, as an index of oxidative stress, and to determine the cellular sources of the measured H2 O2 using the extracellular probe Amplex red (AR). AR was added to the recirculating perfusate in an isolated perfused rat lung. AR's highly fluorescent oxidation product resorufin was measured in the perfusate. Experiments were carried out without and with rotenone (complex I inhibitor), thenoyltrifluoroacetone (complex II inhibitor), antimycin A (complex III inhibitor), potassium cyanide (complex IV inhibitor), or diohenylene iodonium (inhibitor of flavin-containing enzymes, e...
September 28, 2018: Free Radical Research
Nur Fathiah Abdul Sani, Mohd Hanafi Ahmad Damanhuri, Ahmad Imran Zaydi Amir Hamzah, Zulzikry Hafiz Abu Bakar, Jen-Kit Tan, Khairun Nain Nor Aripin, Mohd Dzulkhairi Mohd Rani, Nor Azila Noh, Nor Aripin Shamaan, Rosdinom Razali, Yasmin Anum Mohd Yusof, Musalmah Mazlan, Suzana Makpol, Wan Zurinah Wan Ngah
Ageing is associated with increased oxidative stress accompanied by cognitive decline. The aim of this study was to evaluate oxidative stress biomarkers and their possible relationship with cognitive performances during ageing among the Malay population. Approximately 160 healthy Malay adults aged between 28 and 79 years were recruited around Selangor and Klang Valley. Cognitive function was assessed by Montreal Cognitive Assessment (MoCA), forward digit span (FDS), backward digit span (BDS), digit symbol, Rey Auditory Verbal Learning Test immediate recalled [RAVLT(I)] and delayed recalled [RAVLT(D)], and visual reproduction immediate recalled (VR-I) and delayed recalled (VR-II)...
September 25, 2018: Free Radical Research
Jiankang Liu, Shinya Toyokuni, Young-Joon Surh
No abstract text is available yet for this article.
September 24, 2018: Free Radical Research
Do-Hee Kim, Hyo-Jin Yoon, Young-Nam Cha, Young-Joon Surh
Cancer stem cells (CSCs) constitute a subpopulation of transformed cells that possess intrinsic ability to undergo selfrenewal and differentiation, which drive tumour resistance and cancer recurrence. It has been reported that CSCs possess enhanced protection against oxidative stress induced by reactive oxygen species compared with nonstem-like cancer cells. In the present work, we investigated the role of heme oxygenase-1 (HO-1), a representative antioxidant enzyme, on the stemness and selfrenewal of human breast CSCs...
September 21, 2018: Free Radical Research
Cheng-Di Chiu, You-Pen Chiu, Cheng-Li Lin, Hui-Ru Ji, Chiung-Chyi Shen, Hsu-Tung Lee, Chen Chang
Hyperglycaemia is associated with the poor outcome after intracerebral haemorrhage (ICH). Acetazolamide (AZA), a kind of carbonic anhydrogenase (CA) inhibitor, its effectiveness in ICH had been reported. However, the connections between AZA and ICH, especially in hyperglycaemia condition had never been defined. In this study, adult Sprague-Dawley rats were administered with vehicle or streptozotocin (STZ) to render them into normoglycaemic (NG) or hyperglycaemic (HG), respectively. Collagenase was then injected into the striatum...
September 21, 2018: Free Radical Research
Paul Ames, Tommaso Bucci, Mira Merashli, Marta Amaral, Alessia Arcaro, Fabrizio Gentile, Jaffar Nourooz-Zadeh, Jose DelgadoAlves
Systemic sclerosis (SSc) is a multisystem autoimmune disease: characterized from the clinical side by progressive vasculopathy and fibrosis of the skin and different organs and from the biochemical side by fibroblast deregulation with excessive production of collagen and increased expression of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4). The latter contributes to an overproduction of reactive oxygen species that via an autocrine loop maintains NOX4 in a state of activation. Reactive oxygen and nitrogen species are implicated in the origin and perpetuation of several clinical manifestations of SSc having vascular damage in common; attempts to dampen oxidative and nitrative stress via different agents with antioxidant properties have not translated into sustained clinical benefit...
September 18, 2018: Free Radical Research
Jun Ni, Wenbo Yang, Weifeng Shen, Ruiyan Zhang
It has been reported that heme oxygenase-1 (Hmox1) induction attenuates neointimal thickening. Here we further investigated the potential mechanisms regulating this important pathological process. We revealed that histone deacetylase 2 (HDAC2) was induced following Hmox1 induction under 1% oxygen treatment and this induction was attenuated after the treatment of siRNA against Hmox1. Interestingly, this HDAC2 induction was dependent on Hmox1 protein as well as its enzymatic activity, and was regulated by carbon monoxide released from heme degradation...
September 13, 2018: Free Radical Research
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