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Virchows Archiv: An International Journal of Pathology

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https://www.readbyqxmd.com/read/28220300/application-of-confocal-laser-scanning-microscopy-for-the-diagnosis-of-amyloidosis
#1
Chiara Castellani, Marny Fedrigo, Anna Chiara Frigo, Mila Della Barbera, Gaetano Thiene, Marialuisa Valente, Fausto Adami, Annalisa Angelini
We analysed specificity and sensitivity of confocal laser microscopy (CLSM) on tissue sections for a diagnosis of amyloidosis, in an attempt to reduce technical errors and better standardise pathological diagnosis. We first set up a protocol for the use of CLSM on this type of specimen, using a group of 20 amyloid negative and 20 positive samples. Of all specimens, 2, 4 and 8-μm sections were cut. Sections were stained with Congo red (CR) and thioflavin-T (ThT) and observed by cross-polarised light microscopy (CR-PL), epifluorescence microscopy (CRF-epiFM and ThT-epiFM) and CLSM (CRF-CLSM and ThT-CLSM)...
February 20, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28220299/molecular-analysis-of-braf-v600e-mutation-in-multiple-nodules-of-pulmonary-langerhans-cell-histiocytosis
#2
Arno Dimmler, Helene Geddert, Martin Werner, Gerhard Faller
Pulmonary Langerhans cell histiocytosis (PLCH) is a rare, smoking-related histiocytic disorder with variable clinical symptoms. Like in other non-pulmonary Langerhans cell proliferations, PLCH has recently been shown to harbour BRAF V600E mutations in a significant subset of cases, thus challenging the concept of PLCH being a reactive disorder. Here, we analysed 38 formalin-fixed and paraffin-embedded PLCH nodules of nine patients for BRAF mutation using two different molecular methods. Using pyrosequencing and allele-specific quantitative PCR (AS-PCR), BRAF V600E mutations were found in 16/38 (42%) and 31/37 (84%) nodules, respectively...
February 20, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28220298/response-to-letter-to-the-editor-by-dr-villanacci-toward-optimal-processing-of-endoscopic-submucosal-dissection-specimens
#3
LETTER
Alicia Dessain, Christophe Snauwaert, Pamela Baldin, Pierre Deprez, Louis Libbrecht, Hubert Piessevaux, Anne Jouret-Mourin
No abstract text is available yet for this article.
February 20, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28213807/in-liver-metastases-from-small-intestinal-neuroendocrine-tumors-sstr2a-expression-is-heterogeneous
#4
Mongkon Charoenpitakchai, Eric Liu, Zhiguo Zhao, Tatsuki Koyama, Won Jae Huh, Jordan Berlin, Kenneth Hande, Ronald Walker, Chanjuan Shi
We examined somatostatin receptor type 2A (SSTR2A) expression in primary and metastatic small intestinal neuroendocrine tumors (SI-NETs). We retrieved 156 liver metastases from 26 patients (10 males, 16 females) who had two or more liver lesions resected. A representative formalin-fixed paraffin-embedded section of tumor tissue from each liver metastasis and from the primary tumor, when available, were immunohistochemically stained for SSTR2A. SSTR2A expression was evaluated by the Her2/neu-scoring system and the scoring system proposed by Volante et al...
February 17, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28204871/the-role-of-beta-catenin-mutation-and-sox9-expression-in-sex-cord-stromal-tumours-of-the-testis
#5
F Bremmer, C L Behnes, H U Schildhaus, N T Gaisa, H Reis, H Jarry, H J Radzun, P Stroebel, S Schweyer
The WHO classification of testis tumours includes the group of sex cord-stromal tumours. They are divided into several histological types, i.e. Leydig cell (LCT) and Sertoli cell tumours (SCT). Based on the physiological expression of β-catenin in normal testis/Sertoli cells, it was previously shown that SCT can carry a β-catenin mutation, causing a nuclear positivity for β-catenin and cyclin D1. Furthermore, it could be shown that the stabilization of β-catenin in Sertoli cells causes the loss of the Sertoli cell marker SOX9...
February 16, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28197724/histological-and-immunohistochemical-characteristics-of-undifferentiated-small-round-cell-sarcomas-associated-with-cic-dux4-and-bcor-ccnb3-fusion-genes
#6
Yuichi Yamada, Masaaki Kuda, Kenichi Kohashi, Hidetaka Yamamoto, Junkichi Takemoto, Takeaki Ishii, Kunio Iura, Akira Maekawa, Hirofumi Bekki, Takamichi Ito, Hiroshi Otsuka, Makoto Kuroda, Yumi Honda, Shinji Sumiyoshi, Takeshi Inoue, Naoe Kinoshita, Atsushi Nishida, Kyoko Yamashita, Ichiro Ito, Shizuo Komune, Tomoaki Taguchi, Yukihide Iwamoto, Yoshinao Oda
CIC-DUX4 and BCOR-CCNB3 fusion-gene-associated small round cell sarcomas account for a proportion of pediatric small round cell sarcomas, but their pathological features have not been sufficiently clarified. We reviewed a large number of soft tissue tumors registered at our institution, retrieved the cases of unclassified tumors with a small round cell component, and subjected them to histopathological, immunohistochemical, and gene profile analysis. We reviewed 164 cases of unclassified tumors with a small round cell component and analyzed them by RT-PCR and FISH...
February 14, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28191614/undifferentiated-round-cell-ewing-like-sarcoma-not-always-so-round-nor-ewing-like
#7
EDITORIAL
Wei-Lien Wang, Alexander J Lazar
No abstract text is available yet for this article.
February 13, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28191613/toward-optimal-processing-of-endoscopic-submucosal-dissection-specimens
#8
LETTER
Vincenzo Villanacci, Gabrio Bassotti, Luca Reggiani Bonetti, Antonello Trecca, Moris Cadei
No abstract text is available yet for this article.
February 13, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28191612/high-toll-like-receptor-tlr-9-expression-is-associated-with-better-prognosis-in-surgically-treated-pancreatic-cancer-patients
#9
Joni Leppänen, Olli Helminen, Heikki Huhta, Joonas H Kauppila, Joel Isohookana, Kirsi-Maria Haapasaari, Petri Lehenkari, Juha Saarnio, Tuomo J Karttunen
Pancreatic cancer remains one of the deadliest malignancies in the world. Inflammatory response and tumor environment are thought to play a major role in its pathogenesis. Knowledge on TLR expression and impact on patient survival in pancreatic cancer is limited. The study's aim was to clarify the role of different TLRs in pancreatic cancer. TLR2, TLR4, and TLR9 expression was investigated in 65 surgically resected pancreatic ductal adenocarcinoma specimens by immunohistochemistry. The association between TLR expression, clinical parameters, and local inflammatory response to the tumor was assessed using chi-square test...
February 12, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28188442/patients-with-mccune-albright-syndrome-have-a-broad-spectrum-of-abnormalities-in-the-gastrointestinal-tract-and-pancreas
#10
Laura D Wood, Michaël Noë, Wenzel Hackeng, Lodewijk A A Brosens, Feriyl Bhaijee, Marija Debeljak, Jun Yu, Masaya Suenaga, Aatur D Singhi, Atif Zaheer, Alison Boyce, Cemre Robinson, James R Eshleman, Michael G Goggins, Ralph H Hruban, Michael T Collins, Anne Marie Lennon, Elizabeth A Montgomery
McCune-Albright Syndrome (MAS) is a rare sporadic syndrome caused by post-zygotic mutations in the GNAS oncogene, leading to constitutional mosaicism for these alterations. Somatic activating GNAS mutations also commonly occur in several gastrointestinal and pancreatic neoplasms, but the spectrum of abnormalities in these organs in patients with MAS has yet to be systematically described. We report comprehensive characterization of the upper gastrointestinal tract in seven patients with MAS and identify several different types of polyps, including gastric heterotopia/metaplasia (7/7), gastric hyperplastic polyps (5/7), fundic gland polyps (2/7), and a hamartomatous polyp (1/7)...
February 10, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28188441/factors-that-may-influence-polymorphous-low-grade-adenocarcinoma-growth
#11
Andresa Borges Soares, Elizabeth Ferreira Martinez, Patricia Fernandes Avila Ribeiro, Icleia Siqueira Barreto, Maria Cássia Aguiar, Cristiane Furuse, Marcelo Sperandio, Victor Angelo Montalli, Ney Soares de Araújo, Vera Cavalcanti de Araújo
There is mounting evidence on the importance of some biological processes in tumor growth, such as vascular supply, apoptosis, autophagy, and senescence. We have investigated these processes in polymorphous low-grade adenocarcinoma (PLGA), in an attempt to identify those that are relevant for this particular lesion. We analyzed 31 cases of PLGA using immunohistochemistry to antibodies against CD34 and CD105 to detect blood vessels; against D2-40 to detect lymphatic vessels; against Bax, Bcl-2, and survivin to explore cell apoptosis; and against Beclin and LCB3 to investigate autophagy and against p21 and p16 to assess senescence...
February 10, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28188440/pathology-of-infectious-diseases-what-does-the-future-hold
#12
REVIEW
Paul Hofman, Sebastian Lucas, Grégory Jouvion, Arnault Tauziède-Espariat, Fabrice Chrétien, Gieri Cathomas
The demand for expertise in pathology for the diagnosis of infectious diseases (ID) is continually growing, due to an increase in ID in immunocompromised patients and in the (re)-emergence of common and uncommon diseases, including tropical infections and infections with newly identified microbes. The microbiology laboratory plays a crucial role in diagnosing infections, identifying the responsible infectious agents and establishing sensitivity of pathogens to drug therapy. Pathology, however, is the only way to correlate the presence of an infectious agent with the reaction it evokes at cell and tissue level...
February 10, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28185053/glutaminase-expression-is-a-poor-prognostic-factor-in-node-positive-triple-negative-breast-cancer-patients-with-a-high-level-of-tumor-infiltrating-lymphocytes
#13
Joo Young Kim, Sun-Hee Heo, Seul Ki Choi, In Hye Song, In Ah Park, Young-Ae Kim, Hye Seon Park, Suk Young Park, Won Seon Bang, Gyungyub Gong, Hee Jin Lee
Glutamine metabolism is emerging as one aspect of dysregulated metabolism of tumors. Triple-negative breast cancer (TNBC) cells are glutamine dependent, whereas luminal-type cells tend to be glutamine independent. Therefore, TNBC patients might benefit from therapies targeting glutamine metabolism. To investigate the clinical significance of glutamine metabolism, we examined expression and prognostic significance of glutaminase in tumor cells and tumor-infiltrating lymphocytes (TILs) in TNBC. We retrieved 658 surgically resected TNBCs and analyzed glutaminase expression in tumor cells and TILs by immunohistochemical staining...
February 9, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28168330/whipple-s-disease-in-granulomatous-disguise-a-challenging-diagnosis-with-many-histopathological-pitfalls
#14
Mieke Van Bockstal, Anne Hoorens, Filip Van den Bosch, David Creytens, Sofie Verbeke, Jo Van Dorpe
No abstract text is available yet for this article.
February 6, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28133700/medication-associated-gastrointestinal-tract-injury
#15
REVIEW
Michael Vieth, Elizabeth Montgomery
Medication-associated gastrointestinal (GI) tract injury has been known for centuries for some medications. The more recently introduced biologicals are a class of drugs that constantly increases, and as such, the spectrum of GI tract side effects is steadily growing. This review covers not only long-known GI tract side effects of drugs but also those more recently described. A comprehensive but concise list of medications used in daily practice and associated with GI tract injury is presented.
January 30, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28130699/autopsy-after-transcatheter-aortic-valve-implantation
#16
F van Kesteren, E M A Wiegerinck, S Rizzo, J Baan, R N Planken, J H von der Thüsen, H W M Niessen, M F M van Oosterhout, A Pucci, G Thiene, C Basso, M N Sheppard, K Wassilew, A C van der Wal
Autopsy after transcatheter aortic valve implantation (TAVI) is a new field of interest in cardiovascular pathology. To identify the cause of death, it is important to be familiar with specific findings related to the time interval between the procedure and death. We aimed to provide an overview of the autopsy findings in patients with TAVI in their medical history divided by the timing of death with specific interest in the added value of autopsy over a solely clinically determined cause of death. In 8 European centres, 72 cases with autopsy reports were available...
January 27, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28130698/dna-profiling-of-tumor-buds-in-colorectal-cancer-indicates-that-they-have-the-same-mutation-profile-as-the-tumor-from-which-they-derive
#17
Irene Centeno, Aino Paasinen Sohns, Miriam Flury, José A Galván, Stefan Zahnd, Viktor H Koelzer, Lena Sokol, Heather E Dawson, Alessandro Lugli, Gieri Cathomas, Inti Zlobec
No abstract text is available yet for this article.
January 27, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28116522/molecular-characterization-of-cd44-cd24-ck-cd45-cells-in-benign-and-malignant-breast-lesions
#18
Arnaud Da Cruz Paula, Catarina Leitão, Oriana Marques, Ana Margarida Rosa, Ana Helena Santos, Alexandra Rêma, Maria de Fátima Faria, Ana Rocha, José Luís Costa, Margarida Lima, Carlos Lopes
Breast cancer epithelial cells with the CD44(+)/CD24(-/low) phenotype possess tumor-initiating cells and epithelial-mesenchymal transition (EMT) capacity. Massive parallel sequencing can be an interesting approach to deepen the molecular characterization of these cells. We characterized CD44(+)/CD24(-)/cytokeratin(Ck)(+)/CD45(-) cells isolated through flow cytometry from 43 biopsy and 6 mastectomy samples harboring different benign and malignant breast lesions. The Ion Torrent Ampliseq Cancer Hotspot panel v2 (CHPv2) was used for the identification of somatic mutations in the DNA extracted from isolated CD44(+)/CD24(-)/Ck(+)/CD45(-) cells...
January 23, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28116521/in-hepatic-venous-outflow-obstruction-alcoholic-liver-disease-and-nonalcoholic-fatty-liver-disease-centrilobular-scars-cd34-vessels-and-keratin-7-hepatocytes-are-in-close-proximity
#19
Susumu Matsukuma, Hiroaki Takeo, Yoshitaka Utsumi, Kimiya Sato
For hepatic venous outflow obstruction, alcoholic liver injury, and nonalcoholic fatty liver disease, the term "centrizonal injury disease" (CID) is used, because injury patterns in all three entities are similar. To elucidate CID-related CD34+ vessels (sinusoids and/or microvessels) and keratin 7+ hepatocytes (K7+ Hs), we examined a series of 41 liver tissue specimens obtained at autopsy and surgery, consisting of 32 CID cases and 9 controls. Centrizonal scars were found in 21 CID cases, and these were associated with centrizonal CD34+ vessels (P = 0...
January 23, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28116520/sharing-of-a-ptpn11-mutation-by-myelodysplastic-bone-marrow-and-a-mature-plasmacytoid-dendritic-cell-proliferation-provides-evidence-for-their-common-myelomonocytic-origin
#20
Andrea Bodmer, Thomas Menter, Darius Juskevicius, Christian Arranto, Friedel Wenzel, Stephan Dirnhofer, Alexandar Tzankov
No abstract text is available yet for this article.
January 23, 2017: Virchows Archiv: An International Journal of Pathology
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