Katarina Resman Rus, Samo Zakotnik, Martin Sagadin, Marko Kolenc, Lucijan Skubic, Nataša Knap, Misa Korva, Mario Poljak, Tatjana Avšič-Županc
Monkeypox virus (MPXV), originally endemic in West Africa (Clade II) and Central Africa (Clade I), has recently emerged worldwide and has reinforced the need for rapid and accurate MPXV diagnostics. This review presents and critically discusses the range of virological methods for laboratory diagnosis and characterization of MPXV as well as related lessons learned and practical experience gained from the 2022 Mpox global outbreak. Real-time PCR is currently considered the diagnostic gold standard and ensures accurate and timely confirmation of suspected Mpox cases based on suspicious skin lesions, and digital PCR improves the precision of MPXV DNA quantification...
January 5, 2024: Acta Dermatovenerologica Alpina, Panonica, et Adriatica