journal
MENU ▼
Read by QxMD icon Read
search

Clinical Chemistry

journal
https://www.readbyqxmd.com/read/30021923/circulating-adipocyte-fatty-acid-binding-protein-concentrations-predict-multiple-mortality-outcomes-among-men-and-women-with-diabetes
#1
EDITORIAL
Chi Ho Lee, Chloe Y Y Cheung, Yu Cho Woo, David T W Lui, Michele M A Yuen, Carol H Y Fong, Wing Sun Chow, Aimin Xu, Karen S L Lam
INTRODUCTION: Raised circulating adipocyte fatty acid-binding protein (AFABP) concentrations are associated with various adverse health conditions. However, their relationship with mortality remains to be defined, especially in view of the sexual dimorphism of circulating AFABP concentrations. Here we investigated prospectively whether serum AFABP concentrations predict multiple mortality outcomes in men and women alike, using a large clinic-based cohort of individuals with type 2 diabetes, a condition with raised AFABP concentrations...
July 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/30021922/development-of-a-highly-sensitive-device-for-counting-the-number-of-disease-specific-exosomes-in-human-sera
#2
Yasuaki Kabe, Makoto Suematsu, Satoshi Sakamoto, Miwa Hirai, Ikko Koike, Takako Hishiki, Atsushi Matsuda, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono, Naoko Minegishi, Atsushi Hozawa, Yoshinori Murakami, Michiaki Kubo, Makoto Itonaga, Hiroshi Handa
BACKGROUND: Although circulating exosomes in blood play crucial roles in cancer development and progression, difficulties in quantifying exosomes hamper their application for reliable clinical testing. By combining the properties of nanobeads with optical disc technology, we have developed a novel device named the ExoCounter to determine the exact number of exosomes in the sera of patients with various types of cancer. METHOD: In this system, individual exosomes were captured in the groove of an optical disc coated with antibodies against exosome surface antigens...
July 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/30018057/immuno-maldi-tof-ms-in-the-clinic
#3
EDITORIAL
René P Zahedi, Carol E Parker, Christoph H Borchers
No abstract text is available yet for this article.
July 17, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/30018056/evaluation-of-preanalytical-conditions-and-implementation-of-quality-control-steps-for-reliable-gene-expression-and-dna-methylation-analyses-in-liquid-biopsies
#4
Martha Zavridou, Sofia Mastoraki, Areti Strati, Eleni Tzanikou, Maria Chimonidou, Evi Lianidou
BACKGROUND: Liquid biopsy provides important information for the prognosis and treatment of cancer patients. In this study, we evaluated the effects of preanalytical conditions on gene expression and DNA methylation analyses in liquid biopsies. METHODS: We tested the stability of circulating tumor cell (CTC) messenger RNA by spiking MCF-7 cells in healthy donor peripheral blood (PB) drawn into 6 collection-tube types with various storage conditions. CTCs were enriched based on epithelial cell adhesion molecule positivity, and RNA was isolated followed by cDNA synthesis...
July 17, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/30012641/circulating-proenkephalin-acute-kidney-injury-and-its-improvement-in-patients-with-severe-sepsis-or-shock
#5
Pietro Caironi, Roberto Latini, Joachim Struck, Oliver Hartmann, Andreas Bergmann, Valentina Bellato, Sandra Ferraris, Gianni Tognoni, Antonio Pesenti, Luciano Gattinoni, Serge Masson
BACKGROUND: Acute kidney injury (AKI)9 occurs in many critically ill patients and is associated with high mortality. We examined whether proenkephalin could predict incident AKI and its improvement in septic patients. METHODS: Plasma proenkephalin A 119-159 (penKid) was assayed in 956 patients with sepsis or septic shock enrolled in the multicenter Albumin Italian Outcome Sepsis (ALBIOS) trial to test its association with incident AKI, improvement of renal function, need for renal replacement therapy (RRT), and mortality...
July 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/30002097/detection-and-quantification-of-carbohydrate-deficient-transferrin-by-maldi-compatible-protein-chips-prepared-by-ambient-ion-soft-landing
#6
Petra Darebna, Jan Spicka, Radek Kucera, Ondrej Topolcan, Eva Navratilova, Viktor Ruzicka, Michael Volny, Petr Novak, Petr Pompach
BACKGROUND: Transferrin is synthetized in the liver and is the most important iron-transport carrier in the human body. Severe alcohol consumption leads to alterations in glycosylation of transferrin. Mass spectrometry can provide fast detection and quantification of transferrin isoforms because they have different molecular masses. In this study, we used antibody chips in combination with MALDI-TOF MS for the detection and quantification of transferrin isoforms. METHODS: Protein chips were prepared by functionalization of indium tin oxide glass using ambient ion soft landing of electrosprayed antitransferrin antibody...
July 12, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29991478/genomic-privacy
#7
REVIEW
Abraham P Schwab, Hung S Luu, Jason Wang, Jason Y Park
BACKGROUND: Genetic information is unique among all laboratory data because it not only informs the current health of the specific person tested but may also be predictive of the future health of the individual and, to varying degrees, all biological relatives. CONTENT: As DNA sequencing has become ubiquitous with decreasing cost, large repositories of genomic data have emerged from the domains of research, healthcare, law enforcement, international security, and recreational consumer interest (i...
July 10, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29991477/fecal-microbiota-and-screening-for-colorectal-cancer
#8
EDITORIAL
Meredith E Pittman
No abstract text is available yet for this article.
July 10, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29959148/external-quality-assessment-testing-near-the-limit-of-detection-for-high-sensitivity-cardiac-troponin-assays
#9
LETTER
Peter A Kavsak
No abstract text is available yet for this article.
June 29, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29959147/cardiac-troponin-t-concentrations-reversible-myocardial-ischemia-and-indices-of-left-ventricular-remodeling-in-patients-with-suspected-stable-angina-pectoris-a-doppler-cip-substudy
#10
Peder L Myhre, Torbjørn Omland, Sebastian I Sarvari, Heikki Ukkonen, Frank Rademakers, Jan E Engvall, Tor-Arne Hagve, Eike Nagel, Rosa Sicari, Jose L Zamorano, Mark Monaghan, Jan D'hooge, Thor Edvardsen, Helge Røsjø
BACKGROUND: Cardiac troponin T concentrations measured with high-sensitivity assays (hs-cTnT) provide important prognostic information for patients with stable coronary artery disease (CAD). However, whether hs-cTnT concentrations mainly reflect left ventricular (LV) remodeling or recurrent myocardial ischemia in this population is not known. METHODS: We measured hs-cTnT concentrations in 619 subjects with suspected stable CAD in a prospectively designed multicenter study...
June 29, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29945966/specifying-a-gold-standard-for-the-validation-of-fetal-fraction-estimation-in-prenatal-screening
#11
Nicholas J Wald, King Wai Lau, Jonathan P Bestwick, Robert W Old, Wayne J Huttly, Ray Cheng
BACKGROUND: An estimate of fetal fraction (FF) is needed for DNA-based screening for trisomy 21 and other aneuploidies, but there is no gold standard to validate FF measurement methods. We specify a gold standard and use it to validate 1 method of measuring FF (SeqFF). METHODS: The gold standard was a formula derived from 2 elements: ( a ) an estimate of the percentage of DNA fragments in maternal plasma from chromosome 21 (%Ch21) in pregnancies without trisomy 21, 18, or 13 (PU ) and ( b ) calculation of %Ch21 with increasing FF in trisomy 21 pregnancies (P21 )...
June 26, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29945965/dietary-intakes-and-circulating-concentrations-of-branched-chain-amino-acids-in-relation-to-incident-type-2-diabetes-risk-among-high-risk-women-with-a-history-of-gestational-diabetes-mellitus
#12
Deirdre K Tobias, Clary Clish, Samia Mora, Jun Li, Liming Liang, Frank B Hu, JoAnn E Manson, Cuilin Zhang
BACKGROUND: Circulating branched-chain amino acids (BCAAs; isoleucine, leucine, valine) are consistently associated with increased type 2 diabetes (T2D) risk, but the relationship with dietary intake of BCAAs is less clear. METHODS: The longitudinal Nurses' Health Study II cohort conducted a blood collection from 1996 to 1999. We profiled plasma metabolites among 172 incident T2D cases and 175 age-matched controls from women reporting a history of gestational diabetes before blood draw...
June 26, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941472/eubivas-within-and-between-subject-biological-variation-data-for-electrolytes-lipids-urea-uric-acid-total-protein-total-bilirubin-direct-bilirubin-and-glucose
#13
Aasne K Aarsand, Jorge Díaz-Garzón, Pilar Fernandez-Calle, Elena Guerra, Massimo Locatelli, William A Bartlett, Sverre Sandberg, Thomas Røraas, Ferruccio Ceriotti, Una Ørvim Sølvik, Marit Sverresdotter Sylte, Abdurrahman Coŝkun, Mustafa Serteser, Ibrahim Unsal, Francesca Tosato, Mario Plebani, Niels Jonker, Gerhard Barla, Anna Carobene
BACKGROUND: The European Federation of Clinical Chemistry and Laboratory Medicine European Biological Variation Study (EuBIVAS) has been established to deliver rigorously determined data describing biological variation (BV) of clinically important measurands. Here, EuBIVAS-based BV estimates of serum electrolytes, lipids, urea, uric acid, total protein, total bilirubin, direct bilirubin, and glucose, as well as their associated analytical performance specifications (APSs), are presented...
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941471/the-inadvertent-discovery-of-human-fetal-cell-microchimerism
#14
Diana W Bianchi
No abstract text is available yet for this article.
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941470/enhanced-liver-fibrosis-score-as-a-predictor-of-hepatocellular-carcinoma
#15
LETTER
Wai Mun Loo, George Boon-Bee Goh, Yeli Wang, Jian-Min Yuan, Lizhen Ong, Yock Young Dan, Woon-Puay Koh
No abstract text is available yet for this article.
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941469/clinical-validation-of-a-novel-high-sensitivity-cardiac-troponin-i-assay-for-early-diagnosis-of-acute-myocardial-infarction
#16
Jasper Boeddinghaus, Raphael Twerenbold, Thomas Nestelberger, Patrick Badertscher, Karin Wildi, Christian Puelacher, Jeanne du Fay de Lavallaz, Elif Keser, Maria Rubini Giménez, Desiree Wussler, Nikola Kozhuharov, Katharina Rentsch, Òscar Miró, F Javier Martin-Sanchez, Beata Morawiec, Sabrina Stefanelli, Nicolas Geigy, Dagmar I Keller, Tobias Reichlin, Christian Mueller
BACKGROUND: Clinical performance of the novel high-sensitivity cardiac troponin I (Siemens-hs-cTnI-Centaur) assay is unknown. We aimed to clinically validate the Siemens-hs-cTnI-Centaur assay and develop 0/1-h and 0/2-h algorithms. METHODS: We enrolled patients presenting to the emergency department with symptoms suggestive of acute myocardial infarction (AMI). Final diagnoses were centrally adjudicated by 2 independent cardiologists including all clinical information twice: first, using serial hs-cTnT (Roche-Elecsys, primary analysis); second, using hs-cTnI (Abbott-Architect, secondary analysis) measurements in addition to the clinically applied (hs)-cTn...
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941468/genome-wide-analysis-of-circulating-cell-free-dna-copy-number-detects-active-melanoma-and-predicts-survival
#17
Shobha Silva, Sarah Danson, Dawn Teare, Fiona Taylor, James Bradford, Andrew J G McDonagh, Abdulazeez Salawu, Greg Wells, George J Burghel, Ian Brock, Daniel Connley, Helen Cramp, David Hughes, Nick Tiffin, Angela Cox
BACKGROUND: A substantial number of melanoma patients develop local or metastatic recurrence, and early detection of these is vital to maximize benefit from new therapies such as immune checkpoints or inhibitors of BRAF and MEK. This study explored the use of novel DNA copy-number profiles in circulating cell-free DNA (cfDNA) as a potential biomarker of active disease and survival. PATIENTS AND METHODS: Melanoma patients were recruited from oncology and dermatology clinics in Sheffield, UK, and cfDNA was isolated from stored blood plasma...
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941467/pharmacogenetics-of-warfarin-therapy
#18
Christian T Ruff
No abstract text is available yet for this article.
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29941466/high-sensitivity-cardiac-troponin-based-strategies-for-the-assessment-of-chest-pain-patients-a-review-of-validation-and-clinical-implementation-studies
#19
REVIEW
Kai M Eggers, Tomas Jernberg, Lina Ljung, Bertil Lindahl
BACKGROUND: The introduction of high-sensitivity cardiac troponin (hs-cTn) assays has improved the early assessment of chest pain patients. A number of hs-cTn-based algorithms and accelerated diagnostic protocols (ADPs) have been developed and tested subsequently. In this review, we summarize the data on the performance and clinical utility of these strategies. CONTENT: We reviewed studies investigating the diagnostic and prognostic performance of hs-cTn algorithms [level of detection (LoD) strategy, 0/1-h, 0/2-h, and 0/3-h algorithms) and of hs-cTn-based ADPs, together with the implications of these strategies when implemented as clinical routine...
June 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29921725/preanalytical-processing-and-biobanking-procedures-of-biological-samples-for-metabolomics-research-a-white-paper-community-perspective-for-precision-medicine-and-pharmacometabolomics-task-group-the-metabolomics-society-initiative
#20
REVIEW
Jennifer A Kirwan, Lorraine Brennan, David Broadhurst, Oliver Fiehn, Marta Cascante, Warwick B Dunn, Michael A Schmidt, Vidya Velagapudi
BACKGROUND: The metabolome of any given biological system contains a diverse range of low molecular weight molecules (metabolites), whose abundances can be affected by the timing and method of sample collection, storage, and handling. Thus, it is necessary to consider the requirements for preanalytical processes and biobanking in metabolomics research. Poor practice can create bias and have deleterious effects on the robustness and reproducibility of acquired data. CONTENT: This review presents both current practice and latest evidence on preanalytical processes and biobanking of samples intended for metabolomics measurement of common biofluids and tissues...
June 19, 2018: Clinical Chemistry
journal
journal
31614
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"