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Clinical Chemistry

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https://www.readbyqxmd.com/read/29453194/comparison-of-generic-fluorescent-markers-for-detection-of-extracellular-vesicles-by-flow-cytometry
#1
Leonie de Rond, Edwin van der Pol, Chi M Hau, Zoltan Varga, Auguste Sturk, Ton G van Leeuwen, Rienk Nieuwland, Frank A W Coumans
BACKGROUND: Extracellular vesicles (EVs) in biofluids are potential biomarkers of disease. To explore the clinical relevance of EVs, a specific generic EV marker would be useful, one that does not require antibodies and binds to all EVs. Here we evaluated 5 commonly used generic markers for flow cytometry. METHODS: Flow cytometry (A60-Micro, Apogee) was used to evaluate the ability of the generic EV markers calcein acetoxymethyl ester, calcein acetoxymethyl ester violet, carboxyfluorescein succinimidyl ester (CFSE), 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium (di-8-ANEPPS), and lactadherin to stain EVs from MCF7 human breast adenocarcinoma cell line-conditioned culture medium [epithelial cell adhesion molecule positive (EpCAM + )] or platelet EVs from human plasma [integrin β3 positive (CD61 + )]...
February 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29444902/the-effect-of-single-mismatches-on-primer-extension
#2
Nick A Rejali, Endi Moric, Carl T Wittwer
BACKGROUND: Allele-specific PCR is an important diagnostic tool that identifies single-nucleotide variants by preferential amplification of a particular allele, using primers that are mismatched to all but one allele variant. METHODS: We applied a fluorescent stopped-flow polymerase assay to measure extension rates from oligonucleotide hairpins to simulate primer-template pairs. Under PCR-applicable conditions, reaction rates were recorded in nucleotides per second per polymerase (nt/s/poly)...
February 14, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29440117/fresh-frozen-plasma-transfusion-can-confound-the-analysis-of-circulating-cell-free-dna
#3
LETTER
Su Kah Goh, Vijayaragavan Muralidharan, Christopher Christophi, Alexander Dobrovic
No abstract text is available yet for this article.
February 13, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29436379/advances-in-the-diagnosis-and-management-of-cystic-fibrosis-in-the-genomic-era
#4
REVIEW
Joesph R Wiencek, Stanley F Lo
BACKGROUND: Cystic fibrosis (CF) is a complex autosomal recessive disease that continues to present unique diagnostic challenges. Because CF was first described in 1938, there has been a substantial growth of genetic and phenotypic information about the disorder. During the past few years, as more evidence has become available, a consortium of international experts determined that the 2008 guidelines from the CF Foundation needed to be reviewed and updated. CONTENT: The goal of this review is to highlight the latest advances in CF multidisciplinary care, together with the recent updates to the 2017 CF Foundation diagnostic guidelines...
February 7, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29436378/establishment-of-community-based-reference-intervals-for-fructosamine-glycated-albumin-and-1-5-anhydroglucitol
#5
Elizabeth Selvin, Bethany Warren, Xintong He, David B Sacks, Amy K Saenger
BACKGROUND: There is growing interest in fructosamine, glycated albumin, and 1,5-anhydroglucitol (1,5-AG) as alternative measures of hyperglycemia, particularly for use in settings where traditional measures (glucose and HbA1c) are problematic or where intermediate (2-4 weeks) glycemic control is of interest. However, reference intervals for these alternative biomarkers are not established. METHODS: We measured fructosamine, glycated albumin, and 1,5-AG in a community-based sample of US black and white adults who participated in the Atherosclerosis Risk in Communities (ARIC) Study...
February 7, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29419380/evaluating-rapid-rule-out-of-acute-myocardial-infarction-by-use-of-a-high-sensitivity-cardiac-troponin-i-assay-at-presentation
#6
Jaimi Greenslade, Elizabeth Cho, Christopher Van Hise, Tracey Hawkins, William Parsonage, Jacobus Ungerer, Jillian Tate, Carel Pretorius, Martin Than, Louise Cullen
BACKGROUND: Low concentrations of cardiac troponin (cTn) have been recommended for rapid rule-out of acute myocardial infarction (AMI). We examined the Beckman Coulter Access high-sensitivity cardiac troponin I (hs-cTnI) assay to identify a single test threshold that can safely rule out AMI. METHODS: This analysis used stored samples collected in 2 prospective observational studies. In all, 1871 patients presenting to a tertiary emergency department with symptoms of acute coronary syndrome had blood taken for measurement of cTnI on presentation...
February 1, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29382647/optimizing-amplification-of-the-gc-rich-tert-promoter-region-using-7-deaza-dgtp-for-droplet-digital-pcr-quantification-of-tert-promoter-mutations
#7
LETTER
Andrew J Colebatch, Tom Witkowski, Paul M Waring, Grant A McArthur, Stephen Q Wong, Alexander Dobrovic
No abstract text is available yet for this article.
January 30, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29378689/robust-and-accurate-2-year-performance-of-a-quantitative-mass-spectrometry-based-apolipoprotein-test-in-a-clinical-chemistry-laboratory
#8
LETTER
Renee L Ruhaak, Nico P M Smit, Fred P H T M Romijn, Mervin M Pieterse, Yuri E M van der Burgt, Arnoud van der Laarse, Christa M Cobbaert
No abstract text is available yet for this article.
January 29, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29378688/from-desi-to-the-masspec-pen-ambient-ionization-mass-spectrometry-for-tissue-analysis-and-intrasurgical-cancer-diagnosis
#9
Hannah Marie Brown, Valentina Pirro, R Graham Cooks
No abstract text is available yet for this article.
January 29, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29371231/a-q-a-with-the-qualcomm-tricorder-xprize-winners
#10
Ann M Gronowski, Shannon Haymond, Basil Harris, Chung Kang Peng, Sandeep Sonny S Kohli
No abstract text is available yet for this article.
January 25, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29352044/resolution-of-spurious-immunonephelometric-igg-subclass-measurement-discrepancies-by-lc-ms-ms
#11
Grace van der Gugten, Mari L DeMarco, Luke Y C Chen, Alex Chin, Mollie Caruthers, Daniel T Holmes, Andre Mattman
BACKGROUND: The Binding Site immunonephelometric (IN) IgG subclass reagents (IgG1, IgG2, IgG3, IgG4, BSIN) are used for assessment of both immunodeficiency and IgG4-related disease (IgG4-RD). In our laboratory, suspected analytic errors were noted in patients with increases in IgG4: The sum of the individual IgG subclasses was substantially greater than the measured total IgG concentrations (unlike samples with normal IgG4), and the IgG4 concentration was always less than the IgG2 concentration...
January 19, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29352043/prognostic-implications-of-multiplex-detection-of-kras-mutations-in-cell-free-dna-from-patients-with-pancreatic-ductal-adenocarcinoma
#12
Min Kyeong Kim, Sang Myung Woo, Boram Park, Kyong-Ah Yoon, Yun-Hee Kim, Jungnam Joo, Woo Jin Lee, Sung-Sik Han, Sang-Jae Park, Sun-Young Kong
BACKGROUND: Cell-free DNA (cfDNA) is known to provide potential biomarkers for predicting clinical outcome, but its value in pancreatic ductal adenocarcinoma (PDAC) has not been fully evaluated. The aim of this study was to evaluate the clinical applicability of quantitative analysis of multiplex KRAS mutations in cell-free DNA from patients with PDAC. METHODS: A total of 106 patients with PDAC were enrolled in this prospective study. The concentration and fraction of KRAS mutations were determined through multiplex detection of KRAS mutations in plasma samples by use of a droplet digital PCR kit (Bio-Rad)...
January 19, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29352042/the-enduring-importance-and-challenge-of-commutability
#13
EDITORIAL
Ian S Young
No abstract text is available yet for this article.
January 19, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29348166/clinical-evaluation-of-a-blood-assay-to-diagnose-paucibacillary-tuberculosis-via-bacterial-antigens
#14
Chang Liu, Christopher J Lyon, Yang Bu, Zaian Deng, Elisabetta Walters, Yan Li, Liqun Zhang, Anneke C Hesseling, Edward A Graviss, Ye Hu
BACKGROUND: The diagnosis of active tuberculosis (TB)10 cases primarily relies on methods that detect Mycobacterium tuberculosis (Mtb) bacilli or their DNA in patient samples (e.g., mycobacterial culture and Xpert MTB/RIF assays), but these tests have low clinical sensitivity for patients with paucibacillary TB disease. Our goal was to evaluate the clinical performance of a newly developed assay that can rapidly diagnose active TB cases by direct detection of Mtb-derived antigens in patients' blood samples...
January 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29348165/ifcc-working-group-recommendations-for-assessing-commutability-part-2-using-the-difference-in-bias-between-a-reference-material-and-clinical-samples
#15
Göran Nilsson, Jeffrey R Budd, Neil Greenberg, Vincent Delatour, Robert Rej, Mauro Panteghini, Ferruccio Ceriotti, Heinz Schimmel, Cas Weykamp, Thomas Keller, Johanna E Camara, Chris Burns, Hubert W Vesper, Finlay MacKenzie, W Greg Miller
A process is described to assess the commutability of a reference material (RM) intended for use as a calibrator, trueness control, or external quality assessment sample based on the difference in bias between an RM and clinical samples (CSs) measured using 2 different measurement procedures (MPs). This difference in bias is compared with a criterion based on a medically relevant difference between an RM and CS results to make a conclusion regarding commutability. When >2 MPs are included, the commutability is assessed pairwise for all combinations of 2 MPs...
January 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29348164/ifcc-working-group-recommendations-for-assessing-commutability-part-3-using-the-calibration-effectiveness-of-a-reference-material
#16
Jeffrey R Budd, Cas Weykamp, Robert Rej, Finlay MacKenzie, Ferruccio Ceriotti, Neil Greenberg, Johanna E Camara, Heinz Schimmel, Hubert W Vesper, Thomas Keller, Vincent Delatour, Mauro Panteghini, Chris Burns, W Greg Miller
A process is described to assess the commutability of a reference material (RM) intended for use as a calibrator based on its ability to fulfill its intended use in a calibration traceability scheme to produce equivalent clinical sample (CS) results among different measurement procedures (MPs) for the same measurand. Three sources of systematic error are elucidated in the context of creating the calibration model for translating MP signals to measurand amounts: calibration fit, calibrator level trueness, and commutability...
January 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29348163/ifcc-working-group-recommendations-for-assessing-commutability-part-1-general-experimental-design
#17
W Greg Miller, Heinz Schimmel, Robert Rej, Neil Greenberg, Ferruccio Ceriotti, Chris Burns, Jeffrey R Budd, Cas Weykamp, Vincent Delatour, Göran Nilsson, Finlay MacKenzie, Mauro Panteghini, Thomas Keller, Johanna E Camara, Ingrid Zegers, Hubert W Vesper
Commutability is a property of a reference material (RM) that relates to the closeness of agreement between results for an RM and results for clinical samples (CSs) when measured by ≥2 measurement procedures (MPs). Commutability of RMs used in a calibration traceability scheme is an essential property for them to be fit for purpose. Similarly, commutability of trueness controls or external quality assessment samples is essential when those materials are used to assess trueness of results for CSs. This report is part 1 of a 3-part series describing how to assess commutability of RMs...
January 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29343536/current-and-emerging-multianalyte-assays-with-algorithmic-analyses-are-laboratories-ready-for-clinical-adoption
#18
Jessica M Colón-Franco, Patrick M M Bossuyt, Alicia Algeciras-Schimnich, Christopher Bird, Julia Engstrom-Melnyk, Martin Fleisher, Michael Kattan, Geralyn Lambert-Messerlian
No abstract text is available yet for this article.
January 17, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29343535/debates-in-pain-management-testing
#19
Stacy E F Melanson, William A Clarke, Keith Fragoza, Christopher Gilligan, Rebecca Holdren, Paul J Jannetto, Barbarajean Magnani, Christine Moore
No abstract text is available yet for this article.
January 17, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29343534/effect-of-acute-coronary-syndrome-probability-on-diagnostic-and-prognostic-performance-of-high-sensitivity-cardiac-troponin
#20
Patrick Badertscher, Jasper Boeddinghaus, Thomas Nestelberger, Raphael Twerenbold, Karin Wildi, Zaid Sabti, Christian Puelacher, Maria Rubini Giménez, Julian Pfäffli, Dayana Flores, Jeanne du Fay de Lavallaz, Òscar Miró, F Javier Martin-Sanchez, Beata Morawiec, Jens Lohrmann, Andreas Buser, Dagmar I Keller, Nicolas Geigy, Tobias Reichlin, Christian Mueller
BACKGROUND: There is concern that high-sensitivity cardiac troponin (hs-cTn) may have low diagnostic accuracy in patients with low acute coronary syndrome (ACS) probability. METHODS: We prospectively stratified patients presenting with acute chest discomfort to the emergency department (ED) into 3 groups according to their probability for ACS as assessed by the treating ED physician using a visual analog scale-≤10%, 11% to 79%, and ≥80%-reviewing all information available at 90 min...
January 17, 2018: Clinical Chemistry
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