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Clinical Chemistry

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https://www.readbyqxmd.com/read/29776952/activity-based-concept-to-screen-biological-matrices-for-opiates-and-synthetic-opioids
#1
Annelies Cannaert, Lakshmi Vasudevan, Melissa Friscia, Amanda L A Mohr, Sarah M R Wille, Christophe P Stove
BACKGROUND: Detection of new highly potent synthetic opioids is challenging as new compounds enter the market. Here we present a novel screening method for the detection of opiates and (synthetic) opioids based on their activity. METHODS: A cell-based system was set up in which activation of the μ -opioid receptor (MOR) led to recruitment of β-arrestin 2, resulting in functional complementation of a split NanoLuc luciferase and allowing readout via bioluminescence...
May 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29776951/experimental-design-randomization-and-validation
#2
Keith Baggerly
No abstract text is available yet for this article.
May 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29769179/rna-profiles-of-circulating-tumor-cells-and-extracellular-vesicles-for-therapy-stratification-of-metastatic-breast-cancer-patients
#3
Corinna Keup, Pawel Mach, Bahriye Aktas, Mitra Tewes, Hans-Christian Kolberg, Siegfried Hauch, Markus Sprenger-Haussels, Rainer Kimmig, Sabine Kasimir-Bauer
BACKGROUND: Liquid biopsies are discussed to provide surrogate markers for therapy stratification and monitoring. We compared messenger RNA (mRNA) profiles of circulating tumor cells (CTCs) and extracellular vesicles (EVs) in patients with metastatic breast cancer (MBC) to estimate their utility in therapy management. METHODS: Blood was collected from 35 hormone receptor-positive/HER2-negative patients with MBC at the time of disease progression and at 2 consecutive staging time points...
May 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29760220/quantifying-atherogenic-lipoproteins-current-and-future-challenges-in-the-era-of-personalized-medicine-and-vldl-cholesterol-a-consensus-statement-from-eas-and-eflm
#4
Michel R Langlois, M John Chapman, Christa Cobbaert, Samia Mora, Alan T Remaley, Emilio Ros, Gerald F Watts, Jan Borén, Hannsjörg Baum, Eric Bruckert, Alberico Catapano, Olivier S Descamps, Arnold von Eckardstein, Pia R Kamstrup, Genovefa Kolovou, Florian Kronenberg, Anne Langsted, Kari Pulkki, Nader Rifai, Grazyna Sypniewska, Olov Wiklund, B Rge G Nordestgaard
BACKGROUND: The European Atherosclerosis Society-European Federation of Clinical Chemistry and Laboratory Medicine Consensus Panel aims to provide recommendations to optimize atherogenic lipoprotein quantification for cardiovascular risk management. CONTENT: We critically examined LDL cholesterol, non-HDL cholesterol, apolipoprotein B (apoB), and LDL particle number assays based on key criteria for medical application of biomarkers. ( a ) Analytical performance: Discordant LDL cholesterol quantification occurs when LDL cholesterol is measured or calculated with different assays, especially in patients with hypertriglyceridemia >175 mg/dL (2 mmol/L) and low LDL cholesterol concentrations <70 mg/dL (1...
May 14, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29760219/detectable-high-sensitivity-cardiac-troponin-within-the-population-reference-interval-conveys-high-5-year-cardiovascular-risk-an-observational-study
#5
Martin P Than, Sally J Aldous, Richard W Troughton, Christopher J Pemberton, A Mark Richards, Christopher M A Frampton, Christopher M Florkowski, Peter M George, Samantha Bailey, Joanna M Young, Louise Cullen, Jaimi H Greenslade, William A Parsonage, Brendan M Everett, W Frank Peacock, Allan S Jaffe, John W Pickering
BACKGROUND: Increased cardiac troponin I or T detected by high-sensitivity assays (hs-cTnI or hs-cTnT) confers an increased risk of adverse prognosis. We determined whether patients presenting with putatively normal, detectable cTn concentrations [> limit of detection and < upper reference limit (URL)] have increased risk of major adverse cardiovascular events (MACE) or all-cause mortality. METHODS: A prospective 5-year follow-up of patients recruited in the emergency department with possible acute coronary syndrome (ACS) and cTn concentrations measured with hs-cTnI (Abbott) and hs-cTnT (Roche) assays...
May 14, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29760218/validation-of-an-expanded-carrier-screen-that-optimizes-sensitivity-via-full-exon-sequencing-and-panel-wide-copy-number-variant-identification
#6
Gregory J Hogan, Valentina S Vysotskaia, Kyle A Beauchamp, Stefanie Seisenberger, Peter V Grauman, Kevin R Haas, Sun Hae Hong, Diana Jeon, Shera Kash, Henry H Lai, Laura M Melroy, Mark R Theilmann, Clement S Chu, Kevin Iori, Jared R Maguire, Eric A Evans, Imran S Haque, Rebecca Mar-Heyming, Hyunseok P Kang, Dale Muzzey
BACKGROUND: By identifying pathogenic variants across hundreds of genes, expanded carrier screening (ECS) enables prospective parents to assess the risk of transmitting an autosomal recessive or X-linked condition. Detection of at-risk couples depends on the number of conditions tested, the prevalence of the respective diseases, and the screen's analytical sensitivity for identifying disease-causing variants. Disease-level analytical sensitivity is often <100% in ECS tests because copy number variants (CNVs) are typically not interrogated because of their technical complexity...
May 14, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29691223/-clinical-chemistry-views-and-policy-on-preprints
#7
EDITORIAL
Nader Rifai, Thomas M Annesley, James C Boyd
No abstract text is available yet for this article.
April 24, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29691222/checkpoint-checkmate-microbiota-modulation-of-cancer-immunotherapy
#8
Eric C Keen, Terence S Crofts, Gautam Dantas
No abstract text is available yet for this article.
April 24, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29691221/small-ncrna-seq-results-of-human-tissues-variations-depending-on-sample-integrity
#9
Nicole Ludwig, Tobias Fehlmann, Valentina Galata, Andre Franke, Christina Backes, Eckart Meese, Andreas Keller
BACKGROUND: Although mature miRNAs are relatively stable in vivo, RNA degradation can have a substantial influence on small noncoding RNA (sncRNA) profiles. METHODS: Using different tissue storage conditions and RNA isolation procedures, we analyzed the integrity and quality of RNA isolates from human lung and heart tissues. We sequenced a total of 64 RNA samples and quantified the effect of RNA degradation, DNA contamination, and other confounding factors on the sncRNA-seq data...
April 24, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29685897/characterization-of-human-salivary-extracellular-rna-by-next-generation-sequencing
#10
Feng Li, Karolina Elżbieta Kaczor-Urbanowicz, Jie Sun, Blanca Majem, Hsien-Chun Lo, Yong Kim, Kikuye Koyano, Shannon Liu Rao, So Young Kang, Su Mi Kim, Kyoung-Mee Kim, Sung Kim, David Chia, David Elashoff, Tristan R Grogan, Xinshu Xiao, David T W Wong
BACKGROUND: It was recently discovered that abundant and stable extracellular RNA (exRNA) species exist in bodily fluids. Saliva is an emerging biofluid for biomarker development for noninvasive detection and screening of local and systemic diseases. Use of RNA-Sequencing (RNA-Seq) to profile exRNA is rapidly growing; however, no single preparation and analysis protocol can be used for all biofluids. Specifically, RNA-Seq of saliva is particularly challenging owing to high abundance of bacterial contents and low abundance of salivary exRNA...
April 23, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29661793/cell-free-plasma-dna-for-disease-stratification-and-prognosis-in-head-and-neck-cancer
#11
Markus A Schirmer, Julia Beck, Martin Leu, Michael Oellerich, Margret Rave-Fränk, Philip D Walson, Ekkehard Schütz, Martin Canis
BACKGROUND: Clinicians face many challenges in disease stratification and outcome prediction in head and neck squamous cancer cell (HNSCC) patients. Given the limitations of currently used clinical scoring, repetitive biopsies, and imaging techniques, liquid biopsy approaches may provide valuable additional diagnostic and prognostic information. METHODS: A noninterventional, single-center observational study was performed with clinical data and plasma samples from HNSCC patients...
April 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29653936/atmospheric-pressure-chemical-ionization-is-a-suboptimal-ionization-source-for-steroids
#12
LETTER
Qian Sun, Jianghong Gu, Brian R Stolze, Steven J Soldin
No abstract text is available yet for this article.
April 13, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29653935/high-sensitivity-cardiac-troponin-t-association-of-creatine-kinase-catalytic-activity-with-the-99-th-percentile
#13
LETTER
Annette Masuch, Till Ittermann, Andreas Greinacher, Norbert Lubenow, Thomas Kohlmann, Matthias Nauck, Astrid Petersmann
No abstract text is available yet for this article.
April 13, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29653934/fingerprint-drug-analysis-overcoming-pitfalls-and-heading-toward-the-future
#14
EDITORIAL
Sarah M R Wille
No abstract text is available yet for this article.
April 13, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29653933/reply
#15
LETTER
David R Taylor, Lewis Couchman, Norman F Taylor
No abstract text is available yet for this article.
April 13, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29632129/urine-lipoarabinomannan-for-tuberculosis-diagnosis-evolution-and-prospects
#16
Emily MacLean, Madhukar Pai
No abstract text is available yet for this article.
April 9, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29632128/what-s-new-in-laboratory-test-utilization-management
#17
Geoffrey S Baird, Brian R Jackson, Jane Dickerson, Ken Sikaris, Bernard Croal, Michael Laposata
No abstract text is available yet for this article.
April 9, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29632127/accurate-pan-cancer-molecular-diagnosis-of-microsatellite-instability-by-single-molecule-molecular-inversion-probe-capture-and-high-throughput-sequencing
#18
Adam Waalkes, Nahum Smith, Kelsi Penewit, Jennifer Hempelmann, Eric Q Konnick, Ronald J Hause, Colin C Pritchard, Stephen J Salipante
BACKGROUND: Microsatellite instability (MSI) is an emerging actionable phenotype in oncology that informs tumor response to immune checkpoint pathway immunotherapy. However, there remains a need for MSI diagnostics that are low cost, highly accurate, and generalizable across cancer types. We developed a method for targeted high-throughput sequencing of numerous microsatellite loci with pan-cancer informativity for MSI using single-molecule molecular inversion probes (smMIPs). METHODS: We designed an smMIP panel targeting 111 loci highly informative for MSI across cancers...
April 9, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29632126/development-of-immunochromatographic-assays-for-the-selective-detection-of-zika-virus-or-dengue-virus-serotypes-in-serum
#19
David S Hage
No abstract text is available yet for this article.
April 9, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29632125/full-size-cardiac-troponin-i-and-its-proteolytic-fragments-in-blood-of-patients-with-acute-myocardial-infarction-antibody-selection-for-assay-development
#20
Ivan A Katrukha, Alexander E Kogan, Alexandra V Vylegzhanina, Alexey V Kharitonov, Natalia N Tamm, Vladimir L Filatov, Anastasia V Bereznikova, Ekaterina V Koshkina, Alexey G Katrukha
BACKGROUND: In the blood of patients with acute myocardial infarction (AMI), cardiac troponin I (cTnI) presents as an intact molecule with a repertoire of proteolytic fragments. The degradation of cTnI might negatively influence its precise immunodetection. In this study we identified cTnI fragments and calculated their ratio in the blood of patients at different times after AMI to discriminate the most stable part(s) of cTnI. METHODS: Serial serum samples were collected from AMI patients within 1 to 36 h after the onset of chest pain both before and after stenting...
April 9, 2018: Clinical Chemistry
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