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Clinical Chemistry

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https://www.readbyqxmd.com/read/28724625/amplification-of-misfolded-prion-proteins-in-blood-and-cerebrospinal-fluid-for-detection-of-creutzfeldt-jakob-disease
#1
Mari L DeMarco
No abstract text is available yet for this article.
July 19, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720682/reducing-artifactual-egfr-t790m-mutations-in-dna-from-formalin-fixed-paraffin-embedded-tissue-by-use-of-thymine-dna-glycosylase
#2
Hongdo Do, Ramyar Molania, Paul L Mitchell, Rita Vaiskunaite, John D Murdoch, Alexander Dobrovic
BACKGROUND: False-positive EGFR T790M mutations have been reported in formalin-fixed lung tumors, but the cause of the false positives has not been identified. The T790M mutation results from a C>T change at the cytosine of a CpG dinucleotide. The presence or absence of methylation at this cytosine has different consequences following deamination, resulting in a thymine or uracil, respectively, both of which however result in an artifactual change. Uracil-DNA glycosylase (UDG) can be used to eliminate DNA templates with uracil residues but is not active against artifactual thymines...
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720681/the-eubivas-project-within-and-between-subject-biological-variation-data-for-serum-creatinine-using-enzymatic-and-alkaline-picrate-methods-and-implications-for-monitoring
#3
Anna Carobene, Irene Marino, Abdurrahman Coŝkun, Mustafa Serteser, Ibrahim Unsal, Elena Guerra, William A Bartlett, Sverre Sandberg, Aasne Karine Aarsand, Marit Sverresdotter Sylte, Thomas Røraas, Una Ørvim Sølvik, Pilar Fernandez-Calle, Jorge Díaz-Garzón, Francesca Tosato, Mario Plebani, Niels Jonker, Gerhard Barla, Ferruccio Ceriotti
BACKGROUND: The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) European Biological Variation Study (EuBIVAS) has been established to deliver rigorously determined biological variation (BV) indices. EuBIVAS determined BV for serum creatinine using the enzymatic and alkaline picrate measurement methods. METHOD: In total, 91 healthy individuals (38 males, 53 females; age range, 21-69 years) were bled for 10 consecutive weeks at 6 European laboratories...
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720680/quality-materials-for-quality-assurance-in-the-analysis-of-liquid-biopsy-samples
#4
EDITORIAL
Jason C H Tsang, K C Allen Chan
No abstract text is available yet for this article.
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720679/potential-for-monitoring-gut-microbiota-for-diagnosing-infections-and-graft-versus-host-disease-in-cancer-and-stem-cell-transplant-patients
#5
Andrew Y Koh
BACKGROUND: Gut microbiota, the collective community of microorganisms inhabiting the intestine, have been shown to provide many beneficial functions for the host. Recent advances in next-generation sequencing and advanced molecular biology approaches have allowed researchers to identify gut microbiota signatures associated with disease processes and, in some cases, establish causality and elucidate underlying mechanisms. CONTENT: This report reviews 3 commonly used methods for studying the gut microbiota and microbiome (the collective genomes of the gut microorganisms): 16S rRNA gene sequencing, bacterial group or species-specific quantitative polymerase chain reaction (qPCR), and metagenomic shotgun sequencing (MSS)...
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720678/standardization-of-free-thyroxine-measurements-allows-the-adoption-of-a-more-uniform-reference-interval
#6
Linde A C De Grande, Katleen Van Uytfanghe, Dries Reynders, Barnali Das, James D Faix, Finlay MacKenzie, Brigitte Decallonne, Akira Hishinuma, Bruno Lapauw, Paul Taelman, Paul Van Crombrugge, Annick Van den Bruel, Brigitte Velkeniers, Paul Williams, Linda M Thienpont
BACKGROUND: The IFCC Committee for Standardization of Thyroid Function Tests intended to standardize free thyroxine (FT4) immunoassays. We developed a Système International d'Unités traceable conventional reference measurement procedure (RMP) based on equilibrium dialysis and mass spectrometry. We describe here the latest studies intended to recalibrate against the RMP and supply a proof of concept, which should allow continued standardization efforts. METHODS: We used the RMP to target the standardization and reference interval (RI) panels, which were also measured by 13 manufacturers...
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28720677/synthetic-circulating-cell-free-dna-as-quality-control-materials-for-somatic-mutation-detection-in-liquid-biopsy-for-cancer
#7
Rui Zhang, Rongxue Peng, Ziyang Li, Peng Gao, Shiyu Jia, Xin Yang, Jiansheng Ding, Yanxi Han, Jiehong Xie, Jinming Li
BACKGROUND: Detection of somatic genomic alterations in tumor-derived cell-free DNA (cfDNA) in the plasma is challenging owing to the low concentrations of cfDNA, variable detection methods, and complex workflows. Moreover, no proper quality control materials are available currently. METHODS: We developed a set of synthetic cfDNA quality control materials (SCQCMs) containing spike-in cfDNA on the basis of micrococcal nuclease digestion carrying somatic mutations as simulated cfDNA and matched genomic DNA as genetic background to emulate paired tumor-normal samples in real clinical tests...
July 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28701316/diagnostic-performance-of-high-sensitivity-compared-with-contemporary-cardiac-troponin-i-for-the-diagnosis-of-acute-myocardial-infarction
#8
Yader Sandoval, Stephen W Smith, Sarah E Thordsen, Charles A Bruen, Michelle D Carlson, Kenneth W Dodd, Brian E Driver, Katherine Jacoby, Benjamin K Johnson, Sara A Love, Johanna C Moore, Anne Sexter, Karen Schulz, Nathaniel L Scott, Jennifer Nicholson, Fred S Apple
BACKGROUND: We examined the diagnostic performance of high-sensitivity cardiac troponin I (hs-cTnI) vs contemporary cTnI with use of the 99th percentile alone and with a normal electrocardiogram (ECG) to rule out acute myocardial infarction (MI) and serial changes (deltas) to rule in MI. METHODS: We included consecutive patients presenting to a US emergency department with serial cTnI on clinical indication (NCT02060760). Diagnostic performance for acute MI, including MI subtypes, and 30-day outcomes were examined...
July 12, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28687636/acute-graft-versus-host-disease-prognosis-are-biomarkers-ready-for-preemptive-clinical-trials
#9
Sophie Paczesny
No abstract text is available yet for this article.
July 7, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28687635/tracing-the-seeds-in-the-soil
#10
Stefan Werner, Klaus Pantel
No abstract text is available yet for this article.
July 7, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28687634/laboratory-developed-tests-a-legislative-and-regulatory-review
#11
REVIEW
Jonathan R Genzen, Jeffrey S Mohlman, Jerry L Lynch, Michael W Squires, Ronald L Weiss
BACKGROUND: Twenty-five years ago, the Food and Drug Administration (FDA) asserted in a draft document that "home brew" tests-now commonly referred to as laboratory-developed tests (LDTs)-are subject to the same regulatory oversight as other in vitro diagnostics (IVDs)(4). In 2010, the FDA began work on developing a proposed framework for future LDT oversight. Released in 2014, the draft guidance sparked an intense debate over potential LDT regulation. While the proposed guidance has not been implemented, many questions regarding LDT oversight remain unresolved...
July 7, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28687633/united-states-and-european-multicenter-prospective-study-for-the-analytical-performance-and-clinical-validation-of-a-novel-sensitive-fully-automated-immunoassay-for-calcitonin
#12
George J Kahaly, Alicia Algeciras-Schimnich, Thomas E Davis, Tanja Diana, Joachim Feldkamp, Stefan Karger, Jochem König, Mark A Lupo, Friedhelm Raue, Matthew D Ringel, Jennifer A Sipos, Juergen Kratzsch
BACKGROUND: The objective of this study is the validation and proof of clinical relevance of a novel electrochemiluminescence immunoassay (ECLIA) for the determination of serum calcitonin (CT) in patients with medullary thyroid carcinoma (MTC) and in different diseases of the thyroid and of calcium homeostasis. METHODS: This was a multicenter prospective study on basal serum CT concentrations performed in 9 US and European referral institutions. In addition, stimulated CT concentrations were measured in 50 healthy volunteers after intravenous calcium administration (2...
July 7, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28684553/challenge-to-coordinate-harmonization-activities-on-an-international-level
#13
EDITORIAL
Gary L Myers, W Greg Miller
No abstract text is available yet for this article.
July 6, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28679647/technical-stability-and-biological-variability-in-micrornas-from-dried-blood-spots-a-lung-cancer-therapy-monitoring-showcase
#14
Mustafa Kahraman, Thomas Laufer, Christina Backes, Hannah Schrörs, Tobias Fehlmann, Nicole Ludwig, Jochen Kohlhaas, Eckart Meese, Thomas Wehler, Robert Bals, Andreas Keller
BACKGROUND: Different work flows have been proposed to use miRNAs as blood-borne biomarkers. In particular, the method used for collecting blood from patients can considerably influence the diagnostic results. METHODS: We explored whether dried blood spots (DBSs)(5) facilitate stable miRNA measurements and compared its technical stability with biological variability. First, we tested the stability of DBS samples by generating from 1 person 18 whole-genome-wide miRNA profiles of DBS samples that were exposed to different temperature and humidity conditions...
July 5, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28679646/multiplexed-elimination-of-wild-type-dna-and-high-resolution-melting-prior-to-targeted-resequencing-of-liquid-biopsies
#15
Ioannis Ladas, Mariana Fitarelli-Kiehl, Chen Song, Viktor A Adalsteinsson, Heather A Parsons, Nancy U Lin, Nikhil Wagle, G Mike Makrigiorgos
BACKGROUND: The use of clinical samples and circulating cell-free DNA (cfDNA) collected from liquid biopsies for diagnostic and prognostic applications in cancer is burgeoning, and improved methods that reduce the influence of excess wild-type (WT) portion of the sample are desirable. Here we present enrichment of mutation-containing sequences using enzymatic degradation of WT DNA. Mutation enrichment is combined with high-resolution melting (HRM) performed in multiplexed closed-tube reactions as a rapid, cost-effective screening tool before targeted resequencing...
July 5, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28679645/when-gender-identity-doesn-t-equal-sex-recorded-at-birth-the-role-of-the-laboratory-in-providing-effective-healthcare-to-the-transgender-community
#16
Zil Goldstein, Trevor A Corneil, Dina N Greene
BACKGROUND: Transgender is an umbrella term used to describe individuals who identify with a gender incongruent to or variant from their sex recorded at birth. Affirming gender identity through a variety of social, medical, and surgical interventions is critical to the mental health of transgender individuals. In recent years, awareness surrounding transgender identities has increased, which has highlighted the health disparities that parallel this demographic. These disparities are reflected in the experience of transgender patients and their providers when seeking clinical laboratory services...
July 5, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28667187/impact-of-novel-psychoactive-substances-on-clinical-and-forensic-toxicology-and-global-public-health
#17
Marilyn A Huestis, Simon D Brandt, Suman Rana, Volker Auwärter, Michael H Baumann
No abstract text is available yet for this article.
June 30, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28667186/analytically-sensitive-protein-detection-in-microtiter-plates-by-proximity-ligation-with-rolling-circle-amplification
#18
Tonge Ebai, Felipe Marques Souza de Oliveira, Liza Löf, Lotta Wik, Caroline Schweiger, Anders Larsson, Ulrich Keilholtz, Johannes Haybaeck, Ulf Landegren, Masood Kamali-Moghaddam
BACKGROUND: Detecting proteins at low concentrations in plasma can be crucial for early diagnosis. Current techniques used in clinical routine, such as sandwich ELISA, provide for protein detection because of a dependence on target recognition by pairs of antibodies, but detection of low protein concentrations is often called for. Proximity ligation assay with rolling circle amplification (PLARCA) is an adaptation of the proximity ligation assay (PLA) for analytically specific and sensitive protein detection via binding of target proteins by 3 antibodies, and signal amplification via rolling circle amplification (RCA) in microtiter wells, in a manner easily adapted to instrumentation in use in hospitals...
June 30, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28667185/low-ige-is-insufficiently-sensitive-to-guide-genetic-testing-of-stat3-gain-of-function-mutations
#19
LETTER
Stuart G Tangye, Lisa R Forbes, Jennifer Leiding, Kahn Preece, Ashish R Kumar, Eleonora Gambineri, Joshua D Milner, Megan A Cooper, Mikko Seppänen
No abstract text is available yet for this article.
June 30, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28667184/identification-confirmation-and-replication-of-novel-urinary-microrna-biomarkers-in-lupus-nephritis-and-diabetic-nephropathy
#20
Mariana Cardenas-Gonzalez, Anand Srivastava, Mira Pavkovic, Vanesa Bijol, Helmut G Rennke, Isaac E Stillman, Xiaolan Zhang, Samir Parikh, Brad H Rovin, Maryam Afkarian, Ian H de Boer, Jonathan Himmelfarb, Sushrut S Waikar, Vishal S Vaidya
BACKGROUND: The prevalence of chronic kidney disease (CKD) is increasing, leading to significant morbidity and mortality. Kidney biopsy remains the gold standard for diagnosing the underlying etiology of CKD, but the procedure carries complication risks. The aim of this study was to identify novel noninvasive biomarkers correlating with kidney function and histopathology in biopsy-proven CKD patients. METHODS: We profiled 2402 urinary microRNAs (miRNAs) to identify and confirm differentially expressed miRNAs associated with kidney function and histopathology in patients with diabetic nephropathy (n = 58) or lupus nephritis (n = 89), important etiologies of CKD, compared with healthy controls (n = 93 and 119, respectively)...
June 30, 2017: Clinical Chemistry
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