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Clinical Chemistry

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https://www.readbyqxmd.com/read/28428361/single-tube-dodecaplex-pcr-panel-of-polymorphic-microsatellite-markers-closely-linked-to-the-dmpk-ctg-repeat-for-preimplantation-genetic-diagnosis-of-myotonic-dystrophy-type-1
#1
Mulias Lian, Mingjue Zhao, Caroline G Lee, Samuel S Chong
BACKGROUND: Preimplantation genetic diagnosis (PGD) of myotonic dystrophy type 1 (DM1) currently uses conventional PCR to detect nonexpanded dystrophia myotonica protein kinase (DMPK) alleles or triplet-primed PCR to detect the CTG-expanded alleles, coupled with analysis of linked microsatellite markers to increase diagnostic accuracy. We aimed to simplify the process of identification and selection of informative linked markers for application to DM1 PGD. METHODS: An in silico search was performed to identify all markers within 1-1...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428360/cardiac-troponins-molecules-of-many-surprises
#2
EDITORIAL
Geza S Bodor
No abstract text is available yet for this article.
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428359/in-reply
#3
LETTER
Fred S Apple, Yader Sandoval, Allan S Jaffe
No abstract text is available yet for this article.
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428358/effect-of-repeat-measurements-of-high-sensitivity-cardiac-troponin-on-the-same-sample-using-the-european-society-of-cardiology-0-hour-1-hour-or-2-hour-algorithms-for-early-rule-out-and-rule-in-for-myocardial-infarction
#4
LETTER
https://www.readbyqxmd.com/read/28428357/a-critical-appraisal-of-the-recent-ifcc-statements-on-cardiac-troponin-assays
#5
LETTER
Evangelos Giannitsis, Matthias Mueller-Hennessen
No abstract text is available yet for this article.
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428356/biological-variation-estimates-obtained-from-91-healthy-study-participants-for-9-enzymes-in-serum
#6
Anna Carobene, Thomas Røraas, Una Ørvim Sølvik, Marit Sverresdotter Sylte, Sverre Sandberg, Elena Guerra, Irene Marino, Niels Jonker, Gerhard Barla, William A Bartlett, Pilar Fernandez-Calle, Jorge Díaz-Garzón, Francesca Tosato, Mario Plebani, Abdurrahman Coŝkun, Mustafa Serteser, Ibrahim Unsal, Ferruccio Ceriotti
BACKGROUND: We sought to develop estimates of biological variation (BV) for 9 enzymes in blood serum as part of the European Biological Variation Study. METHODS: Ninety-one healthy study participants (38 male and 53 female, 21-69 years old) were phlebotomized in each of 10 consecutive weeks at 6 European laboratories. The same preanalytical sample-handling protocol was followed at each center before transport to San Raffaele Hospital, Milan, Italy, for analysis...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428355/plasma-urate-cancer-incidence-and-all-cause-mortality-a-mendelian-randomization-study
#7
Camilla J Kobylecki, Shoaib Afzal, Børge G Nordestgaard
BACKGROUND: Observationally, high plasma urate is associated with high risk of cancer. We using a Mendelian randomization design to test the hypothesis that high concentrations of plasma urate are associated with high cancer incidence and all-cause mortality observationally and genetically. METHODS: We performed observational and genetic analyses using plasma urate and the urate solute carrier family 2 member 9 (SLC2A9) rs7442295 genotype in 86210 individuals from the Copenhagen General Population Study...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428354/multiplex-tandem-mass-spectrometry-enzymatic-activity-assay-for-newborn-screening-of-the-mucopolysaccharidoses-and-type-2-neuronal-ceroid-lipofuscinosis
#8
Yang Liu, Fan Yi, Arun Babu Kumar, Naveen Kumar Chennamaneni, Xinying Hong, C Ronald Scott, Michael H Gelb, Frantisek Turecek
BACKGROUND: We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL). METHODS: New substrates were prepared and char-acterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB)...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428353/development-of-a-bnp1-32-immunoassay-that-does-not-cross-react-with-probnp
#9
Lynley K Lewis, Sara D Raudsepp, Tim G Yandle, Timothy C Prickett, A Mark Richards
BACKGROUND: Plasma B-type natriuretic peptide (BNP) concentration reflects cardiac dysfunction and assists in determining the diagnosis and prognosis of heart failure (HF). Current BNP assays overestimate circulating bioactive BNP1-32 concentrations as they also detect less active BNP metabolites and proBNP. A specific BNP1-32 assay with negligible cross-reactivity to proBNP and/or BNP metabolites may be advantageous. METHODS: We developed a Luminex-based specific BNP1-32 immunoassay and compared results obtained from 3 other BNP assays (a Luminex-based total-BNP assay, our BNP RIA, and the commercially available Abbott Architect BNP assay) in plasma from 42 patients with HF and 22 healthy controls...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28428352/thrombin-mediated-degradation-of-human-cardiac-troponin-t
#10
Ivan A Katrukha, Alexander E Kogan, Alexandra V Vylegzhanina, Marina V Serebryakova, Ekaterina V Koshkina, Anastasia V Bereznikova, Alexey G Katrukha
BACKGROUND: Cardiac troponin T (cTnT) is an acknowledged biomarker of acute myocardial infarction (AMI) that is known to be prone to proteolytic degradation in serum. Such degradation is usually explained by the action of μ -calpain, although there could be other candidates for that role. In the current study, we explored the hypothesis that thrombin-mediated cTnT cleavage occurs as a result of the serum sample preparation. METHODS: cTnT degradation was studied by using immunoblotting and mass spectrometry (MS) analysis...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28396562/nonanalytic-laboratory-automation-a-quarter-century-of-progress
#11
Charles D Hawker
No abstract text is available yet for this article.
April 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28396561/analysis-of-biological-interactions-by-affinity-chromatography-clinical-and-pharmaceutical-applications
#12
REVIEW
David S Hage
BACKGROUND: The interactions between biochemical and chemical agents in the body are important in many clinical processes. Affinity chromatography and high-performance affinity chromatography (HPAC), in which a column contains an immobilized biologically related binding agent, are 2 methods that can be used to study these interactions. CONTENT: This review presents various approaches that can be used in affinity chromatography and HPAC to characterize the strength or rate of a biological interaction, the number and types of sites that are involved in this process, and the interactions between multiple solutes for the same binding agent...
April 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28381407/estimating-glomerular-filtration-rate-using-serum-creatinine
#13
Andrew S Levey, John H Eckfeldt
No abstract text is available yet for this article.
April 5, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28377413/quantifying-the-release-of-biomarkers-of-myocardial-necrosis-from-cardiac-myocytes-and-intact-myocardium
#14
Jack Marjot, Thomas E Kaier, Eva D Martin, Shiney S Reji, O'Neal Copeland, Mohammed Iqbal, Bob Goodson, Sarah Hamren, Sian E Harding, Michael S Marber
BACKGROUND: Myocardial infarction is diagnosed when biomarkers of cardiac necrosis exceed the 99th centile, although guidelines advocate even lower concentrations for early rule-out. We examined how many myocytes and how much myocardium these concentrations represent. We also examined if dietary troponin can confound the rule-out algorithm. METHODS: Individual rat cardiac myocytes, rat myocardium, ovine myocardium, or human myocardium were spiked into 400-μ L aliquots of human serum...
April 4, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28348074/prognostic-value-of-inflammatory-and-cardiovascular-biomarkers-for-prediction-of-90-day-all-cause-mortality-after-acute-ischemic-stroke-results-from-the-linz-stroke-unit-study
#15
Benjamin Dieplinger, Christof Bocksrucker, Margot Egger, Christian Eggers, Meinhard Haltmayer, Thomas Mueller
BACKGROUND: Early outcome prediction after acute ischemic stroke is of great interest. The aim of our study was to evaluate the prognostic value of blood biomarkers in patients with acute ischemic stroke. METHODS: We measured interleukin-6 (IL-6), d-dimer, amino-terminal pro-B-type natriuretic peptide (NT-proBNP), high-sensitivity cardiac troponin T, and soluble ST2 plasma concentrations within 24 h after admission to our stroke unit in 721 consecutive acute ischemic stroke patients...
March 27, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28325814/total-analytic-error-for-low-cardiac-troponin-concentrations-%C3%A2-10-ng-l-by-use-of-a-high-sensitivity-cardiac-troponin-assay
#16
LETTER
Peter A Kavsak, Allan S Jaffe, Dina N Greene, Robert H Christenson, Fred S Apple, Alan H B Wu
No abstract text is available yet for this article.
March 21, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28320764/in-reply
#17
LETTER
Toru Suzuki, Liam M Heaney, Donald J L Jones, Leong L Ng
No abstract text is available yet for this article.
March 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28320763/predictive-value-of-nt-probnp-in-patients-with-acute-myocardial-infarction
#18
LETTER
Aldo Clerico, Claudio Passino
No abstract text is available yet for this article.
March 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28320762/fifty-shades-of-yellow
#19
EDITORIAL
Dennis J Dietzen
No abstract text is available yet for this article.
March 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28320761/postprandial-plasma-concentrations-of-proanp-in-patients-with-type-2-diabetes-and-healthy-controls
#20
LETTER
David P Sonne, Dijana Terzic, Filip K Knop, Jens Peter Goetze
No abstract text is available yet for this article.
March 20, 2017: Clinical Chemistry
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