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Clinical Chemistry

Ramy Arnaout
No abstract text is available yet for this article.
September 20, 2018: Clinical Chemistry
Jean-Paul Salameh, Matthew D F McInnes, David Moher, Brett D Thombs, Trevor A McGrath, Robert Frank, Anahita Dehmoobad Sharifabadi, Noémie Kraaijpoel, Brooke Levis, Patrick M Bossuyt
BACKGROUND: We evaluated the completeness of reporting of diagnostic test accuracy (DTA) systematic reviews using the recently developed Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA)-DTA guidelines. METHODS: MEDLINE® was searched for DTA systematic reviews published October 2017 to January 2018. The search time span was modulated to reach the desired sample size of 100 systematic reviews. Reporting on a per-item basis using PRISMA-DTA was evaluated...
September 20, 2018: Clinical Chemistry
Dragana Milosevic, John R Mills, Michael B Campion, Noemi Vidal Folch, Jesse S Voss, Kevin C Halling, W Edward Highsmith, Minetta C Liu, Benjamin R Kipp, Stefan K G Grebe
BACKGROUND: Droplet digital PCR (ddPCR) is an emerging technology for quantitative cell-free DNA oncology applications. However, assay performance criteria must be established in a standardized manner to harness this potential. We reasoned that standard protocols used in clinical chemistry assay validation should be able to fill this need. METHODS: We validated KRAS , EGFR , and BRAF quantitative ddPCR assays based on the Clinical Laboratory Improvement Act regulations for laboratory-developed tests in clinical chemistry and the matching Clinical and Laboratory Standards Institute guidelines...
September 20, 2018: Clinical Chemistry
Yingyu Liu, Karen Ka-Wing Wong, Elaine Yee-Ling Ko, Xiaoyan Chen, Jin Huang, Stephen Kwok-Wing Tsui, Tin Chiu Li, Stephen Siu-Chung Chim
BACKGROUND: A recent study has reported that the microbiota in endometrial fluid of patients receiving in vitro fertilization and embryo transfer (IVF-ET) may predict implantation and pregnancy rates. However, studies are lacking that simultaneously compare the microbiota between endometrial fluid and tissue samples. Whether the microbiota composition in endometrial fluid reflects that in the endometrial tissue remains unclear. METHODS: We systematically profiled the microbiota in endometrial fluid and tissue samples of IVF-ET patients using massively parallel sequencing...
September 20, 2018: Clinical Chemistry
Philip D Adamson, Nicholas L Mills
No abstract text is available yet for this article.
September 20, 2018: Clinical Chemistry
Nasrien E Ibrahim, Rajat Gupta, Asya Lyass, Yiwei Li, Shreya Shrestha, Cian P McCarthy, Hanna K Gaggin, Roland R J van Kimmenade, Joseph M Massaro, Ralph B D'Agostino, James L Januzzi
BACKGROUND: Endothelin-1 (ET-1) is a vasoconstrictor produced by vascular endothelial cells and may play a role in risk for development of coronary artery disease (CAD) and heart failure (HF). In a cohort of 1084 patients referred for coronary angiography, we investigated cross-sectional associations between ET-1 concentrations and prevalent CAD, as well as value of ET-1 for prognostication of future cardiovascular events. METHODS: Associations between ET-1 and presence/ severity of CAD were assessed...
September 13, 2018: Clinical Chemistry
Lucy A Parker, Elisa Chilet-Rosell, Ildefonso Hernández-Aguado, María Pastor-Valero, Sonia Gea, Blanca Lumbreras
BACKGROUND: Despite considerable research investment, moving from biomarker discovery to clinical application has presented unique challenges. We aimed to evaluate progress toward clinical application of a sample of molecular- and "omics"-based diagnostic tests over a 10-year period. METHODS: We used Scopus to locate studies, published before the December 31, 2016, citing 107 original-research articles published in 2006 that assessed the diagnostic value of a molecular- or "omics"-based test...
September 13, 2018: Clinical Chemistry
Melanie L Yarbrough, Carey-Ann D Burnham, Neil W Anderson, Ritu Banerjee, Christine C Ginocchio, Kimberly E Hanson, Timothy M Uyeki
No abstract text is available yet for this article.
September 12, 2018: Clinical Chemistry
Rongrong Huang, Sara Cathey, Laura Pollard, Tim Wood
BACKGROUND: The glycoproteinoses are a subgroup of lysosomal storage diseases (LSDs) resulting from impaired degradation of N-linked oligosaccharide side chains of glycoproteins, which are commonly screened by detecting the accumulated free oligosaccharides (FOSs) in urine via thin layer chromatography (TLC). The traditional TLC method suffers from limited analytical sensitivity and specificity and lacks quantification capability. Therefore, we developed an analytically sensitive and relatively specific assay using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for urinary FOS analysis and validated its use for urine screening of glycoproteinoses and other LSDs...
September 10, 2018: Clinical Chemistry
Glenn E Palomaki, James N Martin, S Ananth Karumanchi, Liona C Poon
No abstract text is available yet for this article.
September 10, 2018: Clinical Chemistry
Graeme Eisenhofer, Jacques W M Lenders
No abstract text is available yet for this article.
September 10, 2018: Clinical Chemistry
Ann M Gronowski
No abstract text is available yet for this article.
August 27, 2018: Clinical Chemistry
Sonia Mansukhani, Louise J Barber, Dimitrios Kleftogiannis, Sing Yu Moorcraft, Michael Davidson, Andrew Woolston, Paula Zuzanna Proszek, Beatrice Griffiths, Kerry Fenwick, Bram Herman, Nik Matthews, Ben O'Leary, Sanna Hulkki, David Gonzalez De Castro, Anisha Patel, Andrew Wotherspoon, Aleruchi Okachi, Isma Rana, Ruwaida Begum, Matthew N Davies, Thomas Powles, Katharina von Loga, Michael Hubank, Nick Turner, David Watkins, Ian Chau, David Cunningham, Stefano Lise, Naureen Starling, Marco Gerlinger
BACKGROUND: Circulating free DNA sequencing (cfDNA-Seq) can portray cancer genome landscapes, but highly sensitive and specific technologies are necessary to accurately detect mutations with often low variant frequencies. METHODS: We developed a customizable hybrid-capture cfDNA-Seq technology using off-the-shelf molecular barcodes and a novel duplex DNA molecule identification tool for enhanced error correction. RESULTS: Modeling based on cfDNA yields from 58 patients showed that this technology, requiring 25 ng of cfDNA, could be applied to >95% of patients with metastatic colorectal cancer (mCRC)...
August 27, 2018: Clinical Chemistry
Erin McElvania
No abstract text is available yet for this article.
August 20, 2018: Clinical Chemistry
Paul Welsh, David Preiss, Anoop S V Shah, David McAllister, Andrew Briggs, Charles Boachie, Alex McConnachie, Caroline Hayward, Sandosh Padmanabhan, Claire Welsh, Mark Woodward, Archie Campbell, David Porteous, Nicholas L Mills, Naveed Sattar
BACKGROUND: Few data compare cardiac troponin T (cTnT) and cardiac troponin I (cTnI) in a general population. We sought to evaluate the distribution and association between cTnT, cTnI, and cardiovascular risk factors in a large general population cohort. METHODS: High-sensitivity cTnT and cTnI were measured in serum from 19501 individuals in the Generation Scotland Scottish Family Health Study. Associations with cardiovascular risk factors were compared using age- and sex-adjusted regression...
August 20, 2018: Clinical Chemistry
Hans C Beck, Lisette O Jensen, Charlotte Gils, Albertine M M Ilondo, Martin Frydland, Christian Hassager, Ole K Møller-Helgestad, Jacob E Møller, Lars M Rasmussen
BACKGROUND: Several plasma proteins have been suggested as markers for a variety of cardiovascular conditions but fail to qualify in independent patient cohorts. This may relate to interference of medication on plasma protein concentrations. We used proteomics to identify plasma proteins that changed in concentration with heparin administration and therefore potentially may confound their evaluation as biomarkers in situations in which heparin is used. METHODS: We used a proteomic approach based on isobaric tagging and nano-LC-MS/MS analysis to quantify several hundred proteins in a discovery study in which individual plasma samples from 9 patients at intravascular ultrasound follow-up 12 months after an acute myocardial infarction before heparin administration and 2, 15, and 60 min after heparin administration; we validated our findings in 500 individual plasma samples obtained at admission from patients with suspected ST segment elevation myocardial infarction (STEMI), of whom 363 were treated with heparin before admission...
August 16, 2018: Clinical Chemistry
Edmund H Wilkes, Gill Rumsby, Gary M Woodward
BACKGROUND: Urine steroid profiles are used in clinical practice for the diagnosis and monitoring of disorders of steroidogenesis and adrenal pathologies. Machine learning (ML) algorithms are powerful computational tools used extensively for the recognition of patterns in large data sets. Here, we investigated the utility of various ML algorithms for the automated biochemical interpretation of urine steroid profiles to support current clinical practices. METHODS: Data from 4619 urine steroid profiles processed between June 2012 and October 2016 were retrospectively collected...
August 10, 2018: Clinical Chemistry
Graeme Eisenhofer, Aleksander Prejbisz, Mirko Peitzsch, Christina Pamporaki, Jimmy Masjkur, Natalie Rogowski-Lehmann, Katharina Langton, Elena Tsourdi, Mariola Pęczkowska, Stephanie Fliedner, Timo Deutschbein, Felix Megerle, Henri J L M Timmers, Richard Sinnott, Felix Beuschlein, Martin Fassnacht, Andrzej Januszewicz, Jacques W M Lenders
BACKGROUND: Measurements of plasma or urinary metanephrines are recommended for diagnosis of pheochromocytoma and paraganglioma (PPGL). What test offers optimal diagnostic accuracy for patients at high and low risk of disease, whether urinary free metanephrines offer advantages over deconjugated metanephrines, and what advantages are offered by including methoxytyramine in panels all remain unclear. METHODS: A population of 2056 patients with suspected PPGLs underwent prospective screening for disease using mass spectrometric-based measurements of plasma free, urinary deconjugated, and urinary free metanephrines and methoxytyramine...
August 10, 2018: Clinical Chemistry
Feng Li, Janice M Yoshizawa, Kyoung-Mee Kim, Julie Kanjanapangka, Tristan R Grogan, Xiaoyan Wang, David E Elashoff, Shigeo Ishikawa, David Chia, Wei Liao, David Akin, Xinmin Yan, Min-Sun Lee, Rayun Choi, Su-Mi Kim, So-Young Kang, Jae-Moon Bae, Tae-Sung Sohn, Jun-Ho Lee, Min-Gew Choi, Byung-Hoon Min, Jun-Haeng Lee, Jae J Kim, Yong Kim, Sung Kim, David T W Wong
BACKGROUND: Biomarkers are needed for noninvasive early detection of gastric cancer (GC). We investigated salivary extracellular RNA (exRNA) biomarkers as potential clinical evaluation tools for GC. METHODS: Unstimulated whole saliva samples were prospectively collected from 294 individuals (163 GC and 131 non-GC patients) who underwent endoscopic evaluation at the Samsung Medical Center in Korea. Salivary transcriptomes of 63 GC and 31 non-GC patients were profiled, and mRNA biomarker candidates were verified with reverse transcription quantitative real-time PCR (RT-qPCR)...
August 10, 2018: Clinical Chemistry
Deborah Mueller, Christian Puelacher, Ursina Honegger, Joan E Walter, Patrick Badertscher, Nicolas Schaerli, Ivo Strebel, Raphael Twerenbold, Jasper Boeddinghaus, Thomas Nestelberger, Christina Hollenstein, Jeanne du Fay de Lavallaz, Raban Jeger, Christoph Kaiser, Damian Wild, Katharina Rentsch, Andreas Buser, Michael Zellweger, Tobias Reichlin, Christian Mueller
BCKGROUND: We aimed to directly compare high-sensitivity cardiac troponin I (hs-cTnI) and high-sensitivity cardiac troponin T (hs-cTnT) in the detection of functionally relevant coronary artery disease (fCAD). METHODS: Consecutive patients referred with clinical suspicion of fCAD and no structural heart disease other than coronary artery disease were included. The presence of fCAD was based on rest/stress myocardial perfusion single-photon emission computed tomography/computed tomography and coronary angiography...
August 10, 2018: Clinical Chemistry
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