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Gene Therapy

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https://www.readbyqxmd.com/read/30013187/characterization-of-a-replicating-expanded-tropism-oncolytic-reovirus-carrying-the-adenovirus-e4orf4-gene
#1
Vera Kemp, Iris J C Dautzenberg, Steve J Cramer, Rob C Hoeben, Diana J M van den Wollenberg
While the mammalian orthoreovirus type 3 dearing (reovirus T3D) infects many different tumour cells, various cell lines resist the induction of reovirus-mediated cell death. In an effort to increase the oncolytic potency, we introduced transgenes into the S1 segment of reovirus T3D. The adenovirus E4orf4 gene was selected as transgene since the encoded E4orf4 protein induces cell death in transformed cells. The induction of cell death by E4orf4 depends in part on its binding to phosphatase 2A (PP2A). In addition to the S1-E4orf4 reovirus, two other reoviruses were employed in our studies...
July 16, 2018: Gene Therapy
https://www.readbyqxmd.com/read/30013186/more-expansive-gene-transfer-to-the-rat-cns-aav-php-eb-vector-dose-response-and-comparison-to-aav-php-b
#2
Robert D Dayton, Mychal S Grames, Ronald L Klein
Engineered recombinant adeno-associated virus (AAV) vectors have advanced the transduction of neurons in the CNS on an expansive, wide-scale basis since the papers first using AAV9 for this purpose. Wide-scale CNS expression is relevant to gene therapy as well as indispensable for basic studies such as disease modeling. For example, the wide-scale gene transfer approach could expedite hypothesis testing in vivo relative to the generation of germ-line transgenic mice for all of the genes of interest. Wide-scale gene transfer is more efficient in neonates than in adults, so improving gene transfer efficiency in adults is an important goal...
July 16, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29973656/downreguation-of-foxm1-by-mir-214-inhibits-proliferation-and-migration-in-hepatocellular-carcinoma
#3
Chuan Tian, Haixia Wu, Chan Li, Xia Tian, Yong Sun, Enqiang Liu, Xiuyong Liao, Wei Song
The FoxM1 transcription factor plays an important role in the progression of HCC. Therefore, it is necessary to study cell regulation of FoxM1. In this study, we determined the expression of miR-214 and it was inversely associated with FoxM1 protein level in HCC; and suppression of FoxM1 translation by miR-214 mimics. We found that miR-214 targeted the 3'untranslated region of FoxM1 mRNA. In addition, the study found that DLX1 was the direct target of FoxM1 in HCC. Downregulation of FoxM1 inhibits the proliferation, migration, and invasion of HCC cells by miR-214...
July 4, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29955127/nanosphere-mediated-co-delivery-of-vegf-a-and-pdgf-b-genes-for-accelerating-diabetic-foot-ulcers-healing-in-rats
#4
Rongfeng Shi, Weishuai Lian, Shilong Han, Chuanwu Cao, Yinpeng Jin, Yifeng Yuan, Hui Zhao, Maoquan Li
Diabetic ischemic ulcer is an intractable diabetic complication. Angiogenesis is a critical factor for wound healing in patients with diabetic foot wounds. Sustained gene delivery could be notably necessary in modulating gene expression in chronic ulcer healing and might be a promising approach for diabetic foot ulcers. In the present study, Sprague-Dawley rats were used to establish diabetic foot ulcer models by streptozotocin and skin biopsy punch. The plasmids expressing VEGF-A and PDGF-B were prepared and then incorporated with polylactic-co-glycolic acid (PLGA) nanospheres to upregulate genes expression...
June 28, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29941984/additive-effects-of-the-combined-expression-of-soluble-forms-of-gas1-and-pten-inhibiting-glioblastoma-growth
#5
Laura Sánchez-Hernández, Justino Hernández-Soto, Paula Vergara, Rosa O González, José Segovia
The overexpression of GAS1 (Growth Arrest Specific 1) in glioma cells induces cell cycle arrest and apoptosis. We previously demonstrated that the apoptotic process set off by GAS1 is caused by its capacity to inhibit the Glial cell-derived neurotrophic factor (GDNF)-mediated intracellular survival signaling pathway. Whereas on the other hand, PTEN is a tumor suppressor, inactive in many tumors, and both GAS1 and PTEN inhibit the PI3K/AKT pathway. Therefore, it is relevant to investigate the potential additive effect of the overexpression of GAS1 and PTEN on tumor growth...
June 25, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29930343/stable-and-reproducible-transgene-expression-independent-of-proliferative-or-differentiated-state-using-bac-tg-embed
#6
Pankaj Chaturvedi, Binhui Zhao, David L Zimmerman, Andrew S Belmont
Reproducible and stable transgene expression is an important goal in both basic research and biotechnology, with each application demanding a range of transgene expression. Problems in achieving stable transgene expression include multi-copy transgene silencing, chromosome-position effects, and loss of expression during long-term culture, induced cell quiescence, and/or cell differentiation. Previously, we described the "BAC TG-EMBED" method for copy-number dependent, chromosome position-independent expression of embedded transgenes within a BAC containing ~170 kb of the mouse Dhfr locus...
June 21, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29925853/lncrna-ftx-sponges-mir-215-and-inhibits-phosphorylation-of-vimentin-for-promoting-colorectal-cancer-progression
#7
Yi Yang, Jinpei Zhang, Xi Chen, Xin Xu, Gang Cao, Hua Li, Tao Wu
Recent researches have reported that long noncoding RNA (lncRNA) five prime to Xist (FTX) plays a crucial role in the initiation and progression of cancers. In the current study, the clinical significance and functional roles of lncRNA FTX in colorectal cancer (CRC) progression were investigated. A significant increase of lncRNA FTX expression in CRC tissue and cell lines was observed. Overexpression of lncRNA FTX was significantly associated with the bigger tumor diameter, the advanced TNM stage, the lymph node, and distant metastasis, and also predicted poor prognosis of patients with CRC...
June 20, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29907877/electroporation-mediated-delivery-of-fer-gene-enhances-innate-immune-response-and-improves-survival-in-a-murine-model-of-pneumonia
#8
Vladislav Dolgachev, Sreehari Panicker, Sanjay Balijepalli, Lane Kelly McCandless, Yue Yin, Samantha Swamy, M V Suresh, Matthew J Delano, Mark R Hemmila, Krishnan Raghavendran, David Machado-Aranda
Previously, we reported that electroporation-mediated (EP) delivery of the FER gene improved survival in a combined trauma-pneumonia model. The mechanism of this protective effect is unknown. In this paper, we performed a pneumonia model in C57/BL6 mice with 500 CFU of Klebsiella pneumoniae. After inoculation, a plasmid encoding human FER was delivered by EP into the lung (PNA/pFER-EP). Survival of FER-treated vs. controls (PNA; PNA/EP-pcDNA) was recorded. In parallel cohorts, bronchial alveolar lavage (BAL) and lung were harvested at 24 and 72 h with markers of infection measured...
June 15, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29907876/in-vitro-evaluation-of-a-lentiviral-two-step-transcriptional-amplification-system-using-gal4ff-transactivator-for-gene-therapy-applications-in-bone-repair
#9
Sofia Bougioukli, Osamu Sugiyama, Ram K Alluri, Robert Yoho, Daniel A Oakes, Jay R Lieberman
In this study, we developed a lentiviral two-step transcriptional amplification (TSTA) system expressing bone morphogenetic protein-2 (BMP-2) under the control of a GAL4FF transactivator to enhance gene expression and limit toxicity for bone repair applications. To this end human MSCs, isolated from bone marrow or adipose tissue, were transduced overnight with a LV-TSTA system (GAL4FF or GAL4vp16) expressing BMP-2 or GFP and evaluated in vitro for transduction efficiency, mean fluorescence intensity, cell viability, and BMP-2 production...
June 15, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29880908/driving-cars-to-the-clinic-for-solid-tumors
#10
LETTER
Mauro Castellarin, Keisuke Watanabe, Carl H June, Christopher C Kloss, Avery D Posey
FDA approval of chimeric antigen receptor T cells (CART cells) is the culmination of several decades of technology development and interrogation of the properties of these gene therapies. CART cells exist as personalized "living drugs" and have demonstrated astounding anti-tumor efficacy in patients with leukemia and lymphoma. However, the future promise of CART efficacy for solid tumors, the greatest unmet burden, is met with a number of challenges that must be surmounted for effective immune responses...
June 7, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29802374/effect-of-pak1-gene-silencing-on-proliferation-and-apoptosis-in-hepatocellular-carcinoma-cell-lines-mhcc97-h-and-hepg2-and-cells-in-xenograft-tumor
#11
Zhi-Lei Zhang, Guang-Chao Liu, Li Peng, Chong Zhang, Yu-Ming Jia, Wu-Han Yang, Lei Mao
This study intends to explore the effect of the PAK1 gene silencing on apoptosis and proliferation of hepatocellular carcinoma (HCC) MHCC97-H and HepG2 cells and cells in xenograft tumor. MHCC97-H and HepG2 cells and mice with xenograft tumor in vivo were randomly divided into control, empty vector and PAK1 shRNA groups. Morphology and the expression of green fluorescent protein of MHCC97-H and HepG2 cells and cells in xenograft tumor were observed. MTT assay and flow cytometry were used to detect proliferation, cell cycle and apoptosis of MHCC97-H and HepG2 cells and cells in xenograft tumor...
May 25, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29789638/human-carbonic-anhydrase-8-aav8-gene-therapy-inhibits-nerve-growth-factor-signaling-producing-prolonged-analgesia-and-anti-hyperalgesia-in-mice
#12
Gerald Z Zhuang, Udita Upadhyay, Xiaoying Tong, Yuan Kang, Diana M Erasso, Eugene S Fu, Konstantinos D Sarantopoulos, Eden R Martin, Tim Wiltshire, Luda Diatchenko, Shad B Smith, William Maixner, Roy C Levitt
Carbonic anhydrase-8 (Car8; murine gene symbol) is an allosteric inhibitor of inositol trisphosphate receptor-1 (ITPR1), which regulates neuronal intracellular calcium release. We previously reported that wild-type Car8 overexpression corrects the baseline allodynia and hyperalgesia associated with calcium dysregulation in the waddle (wdl) mouse due to a 19 bp deletion in exon 8 of the Car8 gene. In this report, we provide preliminary evidence that overexpression of the human wild-type ortholog of Car8 (CA8 WT ), but not the reported CA8 S100P loss-of-function mutation (CA8 MT ), inhibits nerve growth factor (NGF)-induced phosphorylation of ITPR1, TrkA (NGF high-affinity receptor), and ITPR1-mediated cytoplasmic free calcium release in vitro...
April 24, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29686331/car-t-trailblazing-the-path-from-clinical-development-to-the-clinic
#13
EDITORIAL
Samuele Butera
No abstract text is available yet for this article.
April 23, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29961080/car-t-in-the-clinic-drive-with-care
#14
EDITORIAL
Rafael J Yáñez-Muñoz, Stephan A Grupp
No abstract text is available yet for this article.
June 2018: Gene Therapy
https://www.readbyqxmd.com/read/29789639/fludarabine-and-neurotoxicity-in-engineered-t-cell-therapy
#15
Kate L Lowe, Crystal L Mackall, Elliot Norry, Rafael Amado, Bent K Jakobsen, Gwendolyn Binder
Adoptive T-cell therapy, incorporating engineered T cell receptors (TCRs) or chimeric antigen receptors (CARs), target tumor antigens with high affinity and specificity. To increase the potency of adoptively transferred T cells, patients are conditioned with lymphodepleting chemotherapy regimens prior to adoptive T-cell transfer (ACT), and data suggest that fludarabine is an important component of an effective regimen. In a recent clinical trial using CAR-T cells engineered to target the CD19 B-cell antigen to treat acute lymphoblastic leukemia, JCAR-015 (NCT02535364), two patient deaths due to cerebral edema led to trial suspension...
June 2018: Gene Therapy
https://www.readbyqxmd.com/read/29785047/rationally-designed-aav2-and-aavrh8r-capsids-provide-improved-transduction-in-the-retina-and-brain
#16
Jennifer A Sullivan, Lisa M Stanek, Michael J Lukason, Jie Bu, Shayla R Osmond, Elizabeth A Barry, Catherine R O'Riordan, Lamya S Shihabuddin, Seng H Cheng, Abraham Scaria
The successful application of adeno-associated virus (AAV) gene delivery vectors as a therapeutic paradigm will require efficient gene delivery to the appropriate cells in affected organs. In this study, we utilized a rational design approach to introduce modifications to the AAV2 and AAVrh8R capsids and the resulting variants were evaluated for transduction activity in the retina and brain. The modifications disrupted either capsid/receptor binding or altered capsid surface charge. Specifically, we mutated AAV2 amino acids R585A and R588A, which are required for binding to its receptor, heparan sulfate proteoglycans, to generate a variant referred to as AAV2-HBKO...
June 2018: Gene Therapy
https://www.readbyqxmd.com/read/29670247/effects-of-microrna-292-5p-on-myocardial-ischemia-reperfusion-injury-through-the-peroxisome-proliferator-activated-receptor-%C3%AE-%C3%AE-signaling-pathway
#17
Zhen-Dong Zhu, Ji-Yun Ye, Hua Niu, Yu-Mei Ma, Xue-Mei Fu, Zhong-Hua Xia, Xuan Zhang
Ischemia-reperfusion injury (IRI) is a major cause of cardiac damage following various pathological processes, such as free radical damage and cell apoptosis. This study aims to investigate whether microRNA-292-5p (miR-292-5p) protects against myocardial ischemia-reperfusion injury (IRI) via the peroxisome proliferator-activated receptor (PPAR)-α/-γ signaling pathway in myocardial IRI mice models. Mouse models of myocardial IRI were established. Adult male C57BL/6 mice were divided into different groups. The hemodynamic indexes, levels of related inflammatory factors and serum myocardial enzymes, and malondialdehyde (MDA) content and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected...
April 18, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29654247/the-revving-up-of-cars
#18
Muhammad Bilal Abid
No abstract text is available yet for this article.
April 13, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29588497/targeted-in-vivo-knock-in-of-human-alpha-1-antitrypsin-cdna-using-adenoviral-delivery-of-crispr-cas9
#19
Calvin J Stephens, Elena Kashentseva, William Everett, Lyudmila Kaliberova, David T Curiel
Serum deficiency diseases such as alpha-1-antitrypsin deficiency are characterized by reduced function of serum proteins, caused by deleterious genetic mutations. These diseases are promising targets for genetic interventions. Gene therapies using viral vectors have been used to introduce correct copies of the disease-causing gene in preclinical and clinical studies. However, these studies highlighted that disease-alleviating gene expression is lost over time. Integration into a specific chromosomal site could provide lasting therapeutic expression to overcome this major limitation...
April 2018: Gene Therapy
https://www.readbyqxmd.com/read/29567950/understanding-the-molecular-biology-of-intervertebral-disc-degeneration-and-potential-gene-therapy-strategies-for-regeneration-a-review
#20
LETTER
Prasanthi Sampara, Rajkiran Reddy Banala, Satish Kumar Vemuri, Gurava Reddy Av, Subbaiah Gpv
Intervertebral disc degeneration (IVDD) is a multi-factorial process characterized by phenotypic and genotypic changes, which leads to low back pain and disability. Prolonged imbalance between anabolism and catabolism in discs alters their composition resulting in progressive loss of proteoglycans and hydration leading to IVDD. The current managements for IVDD are only able to relieve the symptoms but do not address the underlying pathology of degeneration. Researchers have tried to find out differences between the aging and degeneration of the disc...
April 2018: Gene Therapy
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