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JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology

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https://www.readbyqxmd.com/read/28817943/-maldi-tof-ms-comparison-of-blood-culture-positive-bottle-pretreatment-kit
#1
Takenori Yamashita
Identification of bacteria of sepsis or bacteremia is a useful result for treatment policy. In recent years, bacterial identification has become possible from blood culture bottles by MALDI‒TOF, but it is not as accurate as bacterial identification from agar colonies. Blood culture pretreatment kit (MALDI Sepcityper Kit) is currently on sale from Bruker. However, the current situation has not reached good accuracy. This time, a new blood culture pretreatment kit appeared, so I studied. Up to now, the blood culture pretreatment kit was only MALDI Sepcityper Kit using enzyme digestion method...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28817942/-the-current-status-of-diagnostic-tools-for-leptospirosis
#2
Mitsumasa Saito, Yasuhiko Nikaido, Masahiro Matsumoto, Midori Ogawa, Sharon Y A M Villanueva
Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. The severity of leptospirosis vary from mild, flu-like disease to a more severe form, Weil's disease causing jaundice, hemorrhage, renal failure, and even death. Every year, 300,000‒500,000 cases of severe leptospirosis are reported around the world, with the case fatality rate being 10‒30%. The usual diagnostic tools for leptospirosis are 1) direct observation of leptospires in blood and urine under dark-field microscope, 2) isolation of leptospires from blood, cerebrospinal fluid (CSF), or urine samples by culture, 3) microscopic agglutination test (MAT) to detect anti-Leptospira antibodies in serum, and 4) PCR to detect Leptospira DNA...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28817941/-a-case-of-severe-legionella-longbeachae-pneumonia-and-usefulness-of-lamp-assay
#3
Kumiko Matsushita, Kohei Hijikuro, Shohei Arita, Yu Kaneko, Masahiro Isozaki
Urinary antigen test is frequently used as a routine laboratory test for early diagnosis of Legionella infection, which is especially suitable for ordinary Legionella pneumophila serogroup 1, but not for other types of Legionella. We report a case of severe pneumonia caused by Legionella longbeachae, where a method of loop-mediated isothermal amplification (LAMP) assay contributed an important role for the early detection. This case involved an 83-year-old man who developed fever, dyspnea, and productive cough...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28817940/-confirming-the-utility-of-raisus-antifungal-susceptibility-testing-by-new-software
#4
Tomoko Ono, Hiroyuki Suematsu, Haruki Sawamura, Yuka Yamagishi, Hiroshige Mikamo
Clinical and Laboratory Standards Institute (CLSI) methods for susceptibility tests of yeast are used in Japan. On the other hand, the methods have some disadvantage; 1) reading at 24 and 48 h, 2) using unclear scale, approximately 50% inhibition, to determine MICs, 3) calculating trailing growth and paradoxical effects. These makes it difficult to test the susuceptibility for yeasts. Old software of RAISUS, Ver. 6.0 series, resolved problem 1) and 2) but did not resolve problem 3). Recently, new software of RAISUS, Ver...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28274129/-utility-of-the-rapid-staining-with-the-use-of-microwave-for-detection-of-genus-mycobacterium
#5
Yumiko Funashima, Kyohei Kato, Taeko Narita, Hiroki Hanaiwa, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Kazuyuki Sugahara, Hiroshi Miyamoto
Recently, many laboratories use fluorescence microscopy for rapid screening of clinical specimens for detection of Genus Mycobacterium. The success of the stain depends on the staining temperature at which the fluorescent dye could uniformly penetrate the cell wall through waxy lipid barrier of the mycobacterial organism. Therefore, this process requires a precise heating control. In this study, to control the temperature during fluorescent auramine- rhodamine staining, we explored the potential use of microwave...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28274128/-evaluation-of-a-new-immunochromatographic-kit-for-enhanced-detection-of-influenza-b-virus
#6
Yasushi Ashikawa, Yoshio Takasaki, Shizuo Shindo, Yuji Yamashita, Keigo Shibao, Takashi Yokoyama, Takato Yokoyama, Minako Iwaya, Yumi Kiyomatsu, Hiroshi Miyamoto
We have evaluated a new immunochromatographic kit, "KBM LineCheck Flu AB", which had been developed for enhanced detection of influenza B viruses. Five strains of influenza A and B viruses were tested for reactivity and detection limits of the kit. Compared with the detection limits of commercially available kit of QuickNavi-Flu, "KBM LineCheck Flu AB" showed a nearly equal reactivity to influenza A viruses, but quadruple reactivity to 2 influenza B viruses. Also, "KBM LineCheck Flu AB" exhibited high specificity when tested in 130 influenza-negative culture specimens derived from 24 adult volunteers...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28274127/-development-and-evaluation-of-a-new-selective-culture-medium-kbm-anaero-rs-gnr-for-detection-of-anaerobic-gram-negative-rods
#7
Taeko Narita, Kyohei Kato, Hiroki Hanaiwa, Tetsuhiro Harada, Yumiko Funashima, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Tsukuru Umemura
The laboratory culture methods for isolating drug-resistant pathogens has been the gold standard in medical microbiology, and play pivotal roles in the overall management of infectious diseases. Recently, several reports have emphasized the development of antibiotics-resistance among anaerobic gram-negative rods, especially Genus Bacteroides and Prevotella. Therefore, a selective culture method to detect these pathogens is needed. We developed here the new selective culture medium, termed "KBM Anaero RS-GNR," for detecting anaerobic Gram-negative rods...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28274126/-selection-of-laboratory-procedures-to-detect-toxigenic-by-the-2-step-method
#8
Yoko Tanino, Mai Kodama, Hiroomi Daicho, Yoshito Miyauchi, Towa Yasumoto, Yukiji Yamada, Noriko Kyotani, Satoko Kurahashi, Masaji Ushiyama, Takeshi Kimura, Toshiaki Komori, Yumiko Fujitomo, Masaki Nakanishi, Naohisa Fujita
The 2-step method is an algorithm to detect toxigenic Clostridium difficile. We herein compared the sensitivities and specificities of an enzyme immunoassay (toxin A/B-EIA), toxigenic culture (TC-EIA), Loop-Mediated Isothermal Amplification assay (LAMP), and Xpert C. difficile (Xpert) with the detection of the toxin B gene by a polymerase chain reaction (PCR). The results obtained showed that the sensitivities and specificities of toxin A/B-EIA, Xpert, TC-EIA, and LAMP were 30 and 100%, 87.2 and 100%, 97.5 and 89...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26437475/-abstracts-of-the-27th-annual-meeting-of-the-japanese-society-of-clinical-toxicology-july-4-2015-kanazawa-japan
#9
(no author information available yet)
No abstract text is available yet for this article.
July 2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26790486/retraction-aeromonas-species-infection-with-severe-clinical-manifestation-in-okinawa-japan-association-with-gas-gangrene
#10
(no author information available yet)
Following a strict, extensive, and judicious review, it has become clear that the paper by Nakasone et al deviates from the ethical standard of authorship. Therefore, the Editor-in-Chief, Hiroyuki Nishiyama, has agreed to fully retract this paper from publication and from the published record.
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26635004/-rapid-identification-method-positive-blood-culture-bottles-with-maldi-biotyper
#11
Mina Fukuoka, Hitoshi Miyamoto, Shinobu Murakami, Yuri Tanaka, Takuya Kondo, Tatsuya Nishimiya, Haruhiko Osawa
Identification method positive blood culture bottles with MALDI Biotyper is the most important test on precisely and rapidly, for detamination the bacterial name in sepsis and bacteremia is very significant for decision a cure. This time, we devised a new method "blend" to identify the mixture hypostasis that were come into being by centrifuging blood culture broths and 70% formic acid with MALDI Biotyper (Bruker). This time, we identified 65 samples rapidly with MALDI biotyper by "on plate" and "blend," and verified their effectivity...
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26635003/-evaluating-the-stability-of-loop-mediated-isothermal-amplification-reagents-at-irregular-storage-temperatures-for-on-site-diagnosis
#12
Yasuo Inoshima, Naotaka Ishiguro
Temperature-stability of loop-mediated isothermal amplification (LAMP) reagents was determined for their use in on-site diagnosis, such as in farms/pastures. Bst and Csa DNA polymerases and the reagents that were stored at different temperatures (4 or 25°C) for 1, 2, or 4 days were used for the LAMP assay to detect orf virus DNA as a model. After storage at 4 and 25°C for 2 days, the enzymes and reagents were found to retain sufficient activity to carry out successful DNA amplification. Visual diagnosis was also possible with the reagents (Loopamp Fluorescent Detection Reagent or hydroxy naphthol blue, as well as DNA amplification checker, D-Quick) that were stored for 2 days at different temperatures...
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26635002/-a-case-of-pneumococcal-pneumonia-early-diagnosed-by-detection-kit-rapirun%C3%A2-in-a-dialysis-patient
#13
Mai Kodama, Reina Takemoto, Yumiko Fujitomo, Masaki Nakanishi, Hirotaka Tatsukawa
A 65-year-old woman developed fever and dyspnea and visited the emergency department of our hospital. On arrival she was hypoxic, and chest CT showed bilateral centrilobular nodules, infiltrative shadows and bronchial wall thickening. Gram staining wasn't performed on admission, and the urinary antigen-detection test for Streptococcus pneumoniae couldn't be performed because the patient has suffered from anuria caused by renal failure. Therefore, we conducted S. pneumoniae antigen test (RAPIRUN® Streptococcus pneumoniae) using sputum sample, then rapidly diagnosed pneumococcal pneumonia due to the positive result of the rapid test...
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26302528/-abstracts-of-the-26th-annual-meeting-of-the-association-for-rapid-method-and-automation-in-microbiology-july-26-2014-okayama-japan
#14
(no author information available yet)
No abstract text is available yet for this article.
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/26233961/-an-immunochromatographic-device-for-rapid-detection-of-ribosomal-protein-l7-l12-as-a-diagnostic-test-for-mycoplasma-pneumonia
#15
Hiroshi Itoh
No abstract text is available yet for this article.
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/25537179/-investigation-of-the-procalcitonin-and-microbiology-test-and-antibiotics-situation-from-our-hospital
#16
Kotaro Fujii, Kuniko Yamada, Junko Sano, Dai Mashima, Hiroko Takeda
Procalcitonin (PCT) was first described as a sepsis-associated protein in 1993. PCT is increased in the blood at the time of infection by bacteria. Therefore, it is used as an auxiliary indicator of sepsis diagnosis. In addition, PCT is reduced quickly by antibiotics. And use as a stop or change marker is also expected. We have investigated the antimicrobial use and microbial testing of measurement patient and PCT running performance. Number of requests was 3,387 cases (inpatient 2,649 and outpatient 742 cases) for one year...
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/25537178/-clinical-utility-of-pourmedia-gbs-agar-on-screening-for-vaginal-colonization-of-group-b-streptococcus
#17
Mitsunori Kaneda, Hiromi Nagasaki, Megumi Tasaki, Kiyoshi Kamiyama
Group B Streptococcus (GBS) are normal flora of the vagina and intestinal, but if the pregnant woman was infected with GBS in the vagina, miscarriage or premature would occur or the newborn would be developed to severe GBS infection. It is recommended that the inspection of GBS on all pregnant women by Japan Society of Obstetrics and Gynecology (JSOG) and Center for Disease Control and Prevention (CDC). We examined the comparison of detection rate between Pourmedia GBS agar (Eiken Chemical Co., Ltd.) and Nissui Separated Plate Sheep Blood Agar/BTB Lactose Agar medium (Nissui Pharmaceutical Co...
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/25073199/-evaluation-of-the-sensitivity-of-a-densitometry-system-in-judging-the-result-of-influenza-virus-antigen-detection-kit-using-immunochromatography
#18
COMPARATIVE STUDY
Ikuo Yamaguchi, Tomoe Aoyama, Masaru Yamamoto, Keiko Kinosita, Yumi Ito
Immunochromatography viral antigen-detection kits have become popular in clinical settings in Japan. Influenza virus detection kit is one of them. It is sometimes used in early phase of the disease, combined with the early treatment with anti-influenza drugs. Most of them are invented to visually read the test line on their kits. However, we should be careful about their reliability of them. Sometimes human errors occur at the visual tests, and they have different sensitivities among the kits from different companies...
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/25073198/-rapid-diagnosis-and-viral-infections-topics-interpretation-of-the-results-and-characteristics-of-the-virus-detection
#19
Keiji Iida
No abstract text is available yet for this article.
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/25073197/-contribution-of-rapid-diagnostic-test-in-the-treatment-of-infectious-diseases
#20
Ai Suzaki
According to aging of population and medical progress, the risk and mortality rate of pneumonia have been increased. In the causative organism inspection, smear-culture examination of sputum is the gold standard. When it is difficult to obtain high quality sputum and to culture the causative organism, or when antibiotics are previously administered, we have no choice but to start the treatment with pathogenic bacteria unknown. In these cases, it is necessary to select an appropriate test method for expecting pathogen...
2014: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
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