journal
MENU ▼
Read by QxMD icon Read
search

In Vitro Cellular & Developmental Biology. Animal

journal
https://www.readbyqxmd.com/read/28986713/best-practices-for-naming-receiving-and-managing-cells-in-culture
#1
Yvonne A Reid
One of the first considerations in using an existing cell line or establishing a new a cell line is the detailed proactive planning of all phases of the cell line management. It is necessary to have a well-trained practitioner in best practices in cell culture who has experience in receiving a new cell line into the laboratory, the correct and appropriate use of a cell line name, the preparation of cell banks, microscopic observation of cells in culture, growth optimization, cell count, cell subcultivation, as well as detailed protocols on how to expand and store cells...
October 6, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28940132/formation-of-multiple-oocyte-follicles-in-culture
#2
Alice P Christensen, Emeline Peyrache, Heidy Kaune, Suzannah A Williams
Basement membranes are found in every organ of the body. They provide structure and a selective filter for molecules. The ovary is no different with the follicular basal lamina (FBL) separating the granulosa and theca cells, facilitating regulation of the changing follicular environment providing appropriate conditions for the developing oocyte. The FBL is modified in C1galt1 Mutant mice (C1galt1 (FF):ZP3Cre) resulting from oocyte-specific deletion of C1galt1. Changes in the FBL lead to follicles joining to generate multiple-oocyte follicles (MOFs); where two or more oocytes are contained within a single follicle...
September 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28940125/responses-of-an-american-eel-brain-endothelial-like-cell-line-to-selenium-deprivation-and-to-selenite-selenate-and-selenomethionine-additions-in-different-exposure-media
#3
Sophia R Bloch, John J Kim, Phuc H Pham, Peter V Hodson, Lucy E J Lee, Niels C Bols
The effect of selenium deprivation and addition on the American eel brain endothelial cell line (eelB) was studied in three exposure media: complete growth medium (L15/FBS), serum-free medium (L15), and minimal medium (L15/ex). L15/ex contains only galactose and pyruvate and allowed the deprivation of selenium on cells to be studied. In L15/ex, without any obvious source of selenium, eelB cells survived for at least 7 d, formed capillary-like structures (CLS) on Matrigel, and migrated to heal wounds. Three selenium compounds were added to cultures: selenite, selenate, and selenomethionine (SeMet)...
September 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28916966/evaluation-of-in-vitro-efficacy-of-docetaxel-loaded-calcium-carbonate-aragonite-nanoparticles-dtx-caco3np-on-4t1-mouse-breast-cancer-cell-line
#4
Nahidah Ibrahim Hammadi, Yusuf Abba, Mohd Noor Mohd Hezmee, Intan Shameha Abdul Razak, Aminu Umar Kura, Zuki Abu Bakar Zakaria
Cockle shell-derived calcium carbonate nanoparticles have shown promising potentials as slow drug-releasing compounds in cancer chemotherapy. In this study, we evaluated the in vitro efficacy of docetaxel (DTX)-loaded CaCO3NP on 4T1 cell line. This was achieved by evaluating the following: cytotoxicity using MTT assay, fluorescence imaging, apoptosis with Annexin V assay, cell cycle analysis, scanning (SEM) and transmission electron microscopy (TEM), and scratch assay. Based on the results, DTX-CaCO3NP with a DTX concentration of 0...
September 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28916942/comment-on-effects-of-different-omega-3-sources-fish-oil-krill-oil-and-green-lipped-mussel-against-cytokine-mediated-canine-cartilage-degradation-in-vitro-cell-dev-biol-anim-2017-doi-10-1007-s11626-016-0125-y
#5
LETTER
Stephen P Myers, Chris Oliver
No abstract text is available yet for this article.
September 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28842848/dead-box-helicase-6-ddx6-is-a-new-negative-regulator-for-milk-synthesis-and-proliferation-of-bovine-mammary-epithelial-cells
#6
Zhen Zhen, Minghui Zhang, Xiaohan Yuan, Bo Qu, Yanbo Yu, Xuejun Gao, Youwen Qiu
Milk synthesis of bovine mammary gland is a complex biological process that is regulated by hormones and nutrients, but the mechanism of these regulations still needs further research. DEAD-box helicase 6 (DDX6) is an important member of the RNA helicase family, involved in the regulation of mRNA storage and translation in different systems, but its physiological role and mechanism are largely unclear. In this study, we describe DDX6 as a potentially novel negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells (BMECs)...
August 25, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28842809/%C3%AE-catenin-stabilization-promotes-proliferation-and-increase-in-cardiomyocyte-number-in-chick-embryonic-epicardial-explant-culture
#7
Anisha Polley, Puja Sen, Arunima Sengupta, Santanu Chakraborty
Cardiomyocyte (CM) differentiation from proepicardial organ- (PEO) and embryonic epicardium (eEpi)-derived cells or EPDCs in a developing heart emerges as a wide interest in purview of cardiac repair and regenerative medicine. eEpi originates from the precursor PEO and EPDCs, which contribute to several cardiac cell types including smooth muscle cells, fibroblasts, endothelial cells, and CMs during cardiogenesis. Here in this report, we have analyzed several cardiac lineage-specific marker gene expressions between PEO and eEpi cells...
August 25, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28808859/best-practices-in-cell-culture-an-overview
#8
John M Baust, Gertrude Case Buehring, Lia Campbell, Eugene Elmore, John W Harbell, Raymond W Nims, Paul Price, Yvonne A Reid, Frank Simione
This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells...
August 14, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28936806/simultaneous-application-of-cisplatin-and-static-magnetic-field-enhances-oxidative-stress-in-hela-cell-line
#9
Samaneh Kamalipooya, Parviz Abdolmaleki, Zahra Salemi, Fatemeh Javani Jouni, Jaber Zafari, Homa Soleimani
In this study, we reported the effects of simultaneous application of static magnetic field (SMF) and cisplatin as an anticancer drug on the oxidative stress in human cervical cancer (HeLa) cell line and normal skin fibroblast cells (Hu02). The cells were exposed to different SMF intensities (7, 10, and 15 mT) for 24 and 48 h. IC50 concentrations of cisplatin were obtained by MTT assay. The cytotoxic effects of combined treatment were studied by measuring the intracellular reactive oxygen species content using flow cytometric method and estimation of membrane lipid peroxidation by spectrophotometry...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28849348/thalidomide-induces-apoptosis-in-undifferentiated-human-induced-pluripotent-stem-cells
#10
Saoko Tachikawa, Toshinobu Nishimura, Hiromitsu Nakauchi, Kiyoshi Ohnuma
Thalidomide, which was formerly available commercially to control the symptoms of morning sickness, is a strong teratogen that causes fetal abnormalities. However, the mechanism of thalidomide teratogenicity is not fully understood; thalidomide toxicity is not apparent in rodents, and the use of human embryos is ethically and technically untenable. In this study, we designed an experimental system featuring human-induced pluripotent stem cells (hiPSCs) to investigate the effects of thalidomide. These cells exhibit the same characteristics as those of epiblasts originating from implanted fertilized ova, which give rise to the fetus...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28842839/six2-is-involved-in-gata1-mediated-cell-apoptosis-in-mouse-embryonic-kidney-derived-cell-lines
#11
Hua Xia, Xin Yan, Yamin Liu, Pan Ju, Jianing Liu, Dongsheng Ni, Yuping Gu, Qin Zhou, Yajun Xie
Six2 (Sine oculis homeobox 2), a homeodomain transcription factor, plays a crucial role in the regulation of mammalian nephrogenesis. It is also implicated in numerous biological functions, such as cell proliferation, apoptosis, and migration. However, the underlying regulatory mechanisms of Six2 remain largely unknown. In this study, we predicted that CRX, GATA1, HOXD8, and POU2F2 might target, binding to the promoter region of Six2 (~2000 bp) by bioinformatics analysis. Among the four genes, the predicted binding sequence of GATA1 is most highly conserved across species...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28840528/erratum-to-abstract-p-1003-an-approach-towards-generating-selectable-marker-free-transgenic-insecticidal-chickpea-by-means-of-plumular-meristem-transformation
#12
Gourab Ghosh, Shreeparna Ganguly
No abstract text is available yet for this article.
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28840527/erratum-to-establishment-of-immortalized-murine-mesothelial-cells-and-a-novel-mesothelioma-cell-line
#13
Walter Blum, László Pecze, Emanuela Felley-Bosco, Janine Worthmüller-Rodriguez, Licun Wu, Bart Vrugt, Marc de Perrot, Beat Schwaller
No abstract text is available yet for this article.
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28840512/sub-micromolar-concentrations-of-retinoic-acid-induce-morphological-and-functional-neuronal-phenotypes-in-sk-n-sh-neuroblastoma-cells
#14
Emily Harasym, Nicole McAndrew, George Gomez
Neuroblastoma cells are neural crest derivatives that can differentiate into neuron-like cells in response to exogenous agents, and are known to be particularly sensitive to retinoic acid. The spectrum of neuroblastoma responses, ranging from proliferation, migration, differentiation, or apoptosis, is difficult to predict due to the heterogeneity of these tumors and to the broad effective range of retinoic acid. Our study focused on the effects of nanomolar concentrations of retinoic acid on neuroblastoma differentiation in two cell lines cells: SK-N-SH (HTB-11) and IMR-32...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28710602/eldecalcitol-ed-71-an-analog-of-1%C3%AE-25-oh-2d3-inhibits-the-growth-of-squamous-cell-carcinoma-scc-cells-in-vitro-and-in-vivo-by-down-regulating-expression-of-heparin-binding-protein-17-fibroblast-growth-factor-binding-protein-1-hbp17-fgfbp-1-and-fgf-2
#15
T Shintani, F Takatsu, S N Z Rosli, E Usui, A Hamada, K Sumi, Y Hayashido, S Toratani, Tetsuji Okamoto
Heparin-binding protein 17 (HBp17)/fibroblast growth factor-binding protein-1 (FGFBP-1) was first purified from medium conditioned by A431 cells for its capacity to bind to fibroblast growth factors 1 and 2 (FGF-1 and -2). Among FGF family members, FGF-2 is a potent mitogen for various cell types, including vascular endothelial cells, fibroblasts, and cancer cells such as oral squamous cell carcinoma (OSCC) cells. Besides being well known in bone metabolism, the active form of vitamin D3, i.e., 1α,25(OH)2D3 (1,25D3), was reported to have protective effects for heart disease and cancer...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28707225/establishment-and-characterization-of-a-brain-cell-line-from-sea-perch-lateolabrax-japonicus
#16
Yao Le, Yunlong Li, Yilin Jin, Peng Jia, Kuntong Jia, Meisheng Yi
A continuous cell line, designated LJB, derived from the brain of sea perch (Lateolabrax japonicus) was established. LJB cells have been subcultured for more than 60 times in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum (FBS) since the initial primary culture. LJB cells exhibited maximum growth rate at 28°C in DMEM supplemented with 20% FBS. Cytogenetic analysis indicated that the modal chromosome number was 48, which was identical with the chromosome number of embryonic stem-like cells of sea perch...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28702926/the-stability-evaluation-of-mesenchymal-stem-cells-differentiation-toward-endothelial-cells-by-chemical-and-mechanical-stimulation
#17
Elham Hasanzadeh, Ghassem Amoabediny, Nooshin Haghighipour, Nasim Gholami, Javad Mohammadnejad, Shahrokh Shojaei, Nasim Salehi-Nik
Adipose-derived mesenchymal stem cells (ADSCs) are adult multipotent cells able to differentiate into several cell lineages. Vascular endothelial growth factor (VEGF) and the shear stress associated with blood flow are considered as the most important chemical and mechanical cues that play major roles in endothelial differentiation. However, the stability of endothelial-specific gene expression has not been completely addressed yet. ADSCs in passage 3 were cultured inside the tubular silicon tubes and then exposed to VEGF or shear stress produced in a perfusion bioreactor...
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699139/response-to-comments-on-effects-of-different-omega-3-sources-fish-oil-krill-oil-and-green-lipped-mussel-against-cytokine-mediated-canine-cartilage-degradation-in-vitro-cell-dev-biol-anim-2017-doi-10-1007-s11626-016-0125-y
#18
LETTER
Kittisak Buddhachat, Puntita Siengdee, Siriwadee Chomdej, Kumpanart Soontornvipart, Korakot Nganvongpanit
No abstract text is available yet for this article.
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699137/erratum-to-hepatopancreatic-cells-of-a-stone-crab-menippe-frontalis-from-per%C3%A3%C2%BA-separation-viability-study-and-evaluation-of-lipoperoxidation-against-cadmium-contamination
#19
H A Vitorino, R Y P Alta, E C P Alta, P Ortega
No abstract text is available yet for this article.
October 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28593424/hepatopancreatic-cells-of-a-stone-crab-menippe-frontalis-from-per%C3%A3%C2%BA-separation-viability-study-and-evaluation-of-lipoperoxidation-against-cadmium-contamination
#20
H A Vitorino, R Y P Alta, E C P Alta, P Ortega
Crustaceans are frequently used as bioindicators, and changes in their metabolism at the hepatopancreas (HP) level are often followed in these studies. The HP is the site of digestion, absorption, nutrient storage, and toxic metal detoxification, enabling crabs to survive in metal contaminated regions. Cellular damage and high lipid peroxidation (LPO) levels have been found in crab populations under high cadmium (Cd) concentrations. The aim of this work was to separate and characterize the HP cells of the stone crab Menippe frontalis from the Pacific Ocean, Perú (5° 5' 21″ S-81° 6' 51″ W) and to measure the cellular viability and LPO after exposure to the non-essential metal Cd...
October 2017: In Vitro Cellular & Developmental Biology. Animal
journal
journal
31577
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"