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In Vitro Cellular & Developmental Biology. Animal

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https://www.readbyqxmd.com/read/28078501/the-tissue-culture-laboratory-of-dr-george-otto-gey-60%C3%A2-yrs-ago-as-recalled-by-a-former-student
#1
Charles T Ambrose
George Otto Gey was a pioneer in tissue culture, having introduced the roller drum, the HeLa cell line, and the use of human fetal cord serum and beef embryo extract. During his career (1920s-1960s), the field of tissue culture was in its infancy and not yet dependent upon commercial biological supply houses. While the early techniques of cell culture have been greatly improved upon, of historical interest may be personal observations of the Gey Tissue Culture Laboratory, Johns Hopkins Medical School, as recalled by a medical student working there in the 1950s...
January 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28078500/effects-of-different-omega-3-sources-fish-oil-krill-oil-and-green-lipped-mussel-against-cytokine-mediated-canine-cartilage-degradation
#2
Kittisak Buddhachat, Puntita Siengdee, Siriwadee Chomdej, Kumpanart Soontornvipart, Korakot Nganvongpanit
Our purpose was to evaluate the protective effect of three marine omega-3 sources, fish oil (FO), krill oil (KO), and green-lipped mussel (GLM) against cartilage degradation. Canine cartilage explants were stimulated with either 10 ng/mL interleukin-1β (IL-1β) or IL-1β/oncostatin M (10 ng/mL each) and then treated with various concentrations of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA; 3 and 30 μg/mL), FO, KO, or GLM (250, 500, and 1000 μg/mL) for 28 days. Gene expression was then investigated in primary canine chondrocytes...
January 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28039621/establishment-and-characterization-of-caspian-horse-fibroblast-cell-bank-in-iran
#3
Abdolreza Daneshvar Amoli, Nazanin Mohebali, Parvaneh Farzaneh, Seyed Abolhassan Shahzadeh Fazeli, Laleh Nikfarjam, Sepideh Ashouri Movasagh, Zahra Moradmand, Meysam Ganjibakhsh, Ahmad Nasimian, Mehrnaz Izadpanah, Faezeh Vakhshiteh, Neda Sadat Gohari, Najmeh Sadat Masoudi, Maryam Farghadan, Shiva Mohamadi Moghanjoghi, Masoud Khalili, Kourosh J Khaledi
Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0...
December 30, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28039620/primordial-germ-cell-differentiation-of-nuclear-transfer-embryonic-stem-cells-using-surface-modified-electroconductive-scaffolds
#4
Tarlan Eslami-Arshaghi, Saeid Vakilian, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi, Masoud Soleimani, Mohammad Salehi
A combination of nanotopographical cues and surface modification of collagen and fibronectin is a potential platform in primordial germ cells (PGCs) differentiation. In the present study, the synergistic effect of nanotopography and surface modification on differentiation of nuclear transfer embryonic stem cells (nt-ESCs) toward PGC lineage was investigated. In order to achieve this goal, poly-anyline (PANi) was mix within poly-L-lactic acid (PLLA). Afterward, the random composite mats were fabricated using PLLA and PANi mix solution...
December 30, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28039619/labeling-of-adipose-derived-stem-cells-with-quantum-dots-provides-stable-and-long-term-fluorescent-signal-for-ex-vivo-cell-tracking
#5
Clautina R M Costa, Matheus L T Feitosa, Dayseanny O Bezerra, Yulla K P Carvalho, Rodrigo F G Olivindo, Pablo B Fernando, Gustavo C Silva, Mirna L G Silva, Carlos E Ambrósio, Airton M Conde Júnior, Napoleão M Argolo Neto, Laís M Costa Silva, Maria A M Carvalho
Stem cells derived from adipose tissue (ADSC) have been used in cell therapy as an alternative to treat chronic and degenerative diseases. Using biomedical and image trials to track the cells when infused in the target tissue is essential to control cell migration and adhesion. The objective of the present study was to label and assess the adhesion of goat adipose tissue-derived stem cells (g-ADSC) after cell infusion in animal models by tracking luminescent intracytoplasmatic nanocrystals. The cells were labeled by using Qdots...
December 30, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27981423/establishment-and-characterization-of-a-mid-kidney-cell-line-derived-from-golden-pompano-trachinotus-ovatus-a-new-cell-model-for-virus-pathogenesis-and-toxicology-studies
#6
Lingli Zhou, Pengfei Li, Jiaxin Liu, Songwei Ni, Yepin Yu, Min Yang, Shina Wei, Qiwei Qin
Golden pompano Trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. However, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in T. ovatus aquaculture recently. In our research, we established a new mid-kidney cell line, designated as TOK, from golden pompano, T. ovatus. The optimized growth temperature and working concentration of fetal bovine serum (FBS) were 28°C and 10-20%, respectively...
December 15, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27924445/growth-arrest-of-vascular-smooth-muscle-cells-in-suspension-culture-using-low-acyl-gellan-gum
#7
Tomomi Natori, Masachika Fujiyoshi, Masashi Uchida, Natsuki Abe, Tatsuro Kanaki, Yasunori Fukumoto, Itsuko Ishii
The proliferation of vascular smooth muscle cells (SMCs) causes restenosis in biomaterial vascular grafts. The purposes of this study were to establish a suspension culture system for SMCs by using a novel substrate, low-acyl gellan gum (GG) and to maintain SMCs in a state of growth inhibition. When SMCs were cultured in suspension with GG, their proliferation was inhibited. Their viability was 70% at day 2, which was maintained at more than 50% until day 5. In contrast, the viability of cells cultured in suspension without GG was 5...
December 6, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27864663/mesenchymal-stem-cells-attenuate-hydrogen-peroxide-induced-oxidative-stress-and-enhance-neuroprotective-effects-in-retinal-ganglion-cells
#8
Yi Cui, Nuo Xu, Wei Xu, Guoxing Xu
The apoptosis of retinal ganglion cells leads to visual impairment and blindness in ocular neurodegenerative diseases, especially in diabetic retinopathy (DR). Mounting evidence suggests that oxidative stress contributes to the pathogenesis of DR. In the present study, we investigated whether bone mesenchymal stem cells (BMSCs) have protective ability to relieve hydrogen peroxide (H2O2)-induced injury on retinal ganglion cells in vitro. An immortalized retinal ganglion cells, RGC-5 cells, were exposed to an indicated concentration of H2O2 for 24 h...
November 18, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27864662/erratum-to-plant-posters
#9
(no author information available yet)
No abstract text is available yet for this article.
November 18, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27844419/incorrect-strain-information-for-mouse-cell-lines-sequential-influence-of-misidentification-on-sublines
#10
Kozue Uchio-Yamada, Fumio Kasai, Midori Ozawa, Arihiro Kohara
Misidentification or cross-contamination of cell lines can cause serious issues. Human cell lines have been authenticated by short tandem repeat profiling; however, mouse cell lines have not been adequately assessed. In this study, mouse cell lines registered with the JCRB cell bank were examined by simple sequence length polymorphism (SSLP) analysis to identify their strains. Based on comparisons with 7 major inbred strains, our results revealed their strains in 80 of 90 cell lines. However, 12 of the 80 cell lines (15%) were found to differ from registered information...
November 14, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27844418/erratum-to-down-regulation-of-mir-181a-can-reduce-heat-stress-damage-in-pbmcs-of-holstein-cows
#11
Kun-Lin Chen, Yuan-Yuan Fu, Min-Yan Shi, Hui-Xia Li
No abstract text is available yet for this article.
November 14, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27834044/the-influence-of-acute-hypoxia-on-the-functional-and-morphological-state-of-the-black-scorpionfish-red-blood-cells
#12
Aleksandra Y Andreyeva, Aleksander A Soldatov, Vladimir S Mukhanov
The investigation of the mechanisms of red blood cell steadiness to the oxygen lack in tolerant teleosts is of current scientific interest. Black scorpionfish, Scorpaena porcus L., is a widespread benthal species in the Black Sea and is highly resistant to hypoxic influence. The morphological state of black scorpionfish red blood cells under acute hypoxia was assessed using DNA-binding dye SYBR Green I and fluorescent microscopy. Changes in membrane potential of mitochondria and functional activity of cells were determined by rhodamine 123 (R123) and fluorescein diacetate (FDA) fluorescence...
November 10, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27826797/the-cohesion-stabilizer-sororin-favors-dna-repair-and-chromosome-segregation-during-mouse-oocyte-meiosis
#13
Chun-Jie Huang, Yi-Feng Yuan, Di Wu, Faheem Ahmed Khan, Xiao-Fei Jiao, Li-Jun Huo
Maintenance and timely termination of cohesion on chromosomes ensures accurate chromosome segregation to guard against aneuploidy in mammalian oocytes and subsequent chromosomally abnormal pregnancies. Sororin, a cohesion stabilizer whose relevance in antagonizing the anti-cohesive property of Wings-apart like protein (Wapl), has been characterized in mitosis; however, the role of Sororin remains unclear during mammalian oocyte meiosis. Here, we show that Sororin is required for DNA damage repair and cohesion maintenance on chromosomes, and consequently, for mouse oocyte meiotic program...
November 8, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27822884/lncrna-ak015322-promotes-proliferation-of-spermatogonial-stem-cell-c18-4-by-acting-as-a-decoy-for-microrna-19b-3p
#14
Ke Hu, Jing Zhang, Meng Liang
Long noncoding RNAs (lncRNAs) have been reported to play important roles in male reproduction. In our previous research, we studied the expression profile of lncRNAs in mouse male germ cells including spermatogonial stem cell, type A spermatogonia, pachytene spermatocyte, and round spermatid by microarray method, which showed that testis-enriched lncRNA AK015322 is highly expressed in spermatogonial stem cell. In this study, we found that AK015322 promotes proliferation of mouse spermatogonial stem cell line C18-4 in vitro...
November 7, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27815660/mitochondrial-phosphatase-pgam5-regulates-keap1-mediated-bcl-xl-degradation-and-controls-cardiomyocyte-apoptosis-driven-by-myocardial-ischemia-reperfusion-injury
#15
Chen Yang, Xiaojuan Liu, Fangfang Yang, Weixun Zhang, Zihao Chen, Daliang Yan, Qingsheng You, Xiang Wu
Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial membrane protein that plays crucial roles in necroptosis and apoptosis. Though PGAM5 is known to be required for inducing intrinsic apoptosis through interacting with BCL2 associated X protein (Bax) and dynamin-related protein 1 (Drp1), the expression and role of PGAM5 in cardiomyocyte apoptosis driven by myocardial ischemia/reperfusion injury(MIRI) has not been studied. The present study shows that PGAM5 expression decreased after MIRI in vivo, positively correlated with Bcl-xL expression, negatively correlated with Kelch-ECH associating protein 1 (Keap1) expression...
November 4, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27800570/mir-143-regulates-proliferation-and-differentiation-of-bovine-skeletal-muscle-satellite-cells-by-targeting-igfbp5
#16
Wei Ran Zhang, Hui Na Zhang, Yi Min Wang, Yang Dai, Xin Feng Liu, Xin Li, Xiang Bin Ding, Hong Guo
Development of skeletal muscle is a complicated biological process regulated by various regulation factors and signal pathways. MicroRNAs (miRNAs) are novel gene regulators that control muscle cell development. microRNA-143 (miR-143) is highly expressed in skeletal muscle, and we found that miR-143 level is significantly increased during bovine skeletal muscle satellite cells (MSCs) differentiation process through microarray analysis and qRT-PCR detection. However, the function of miR-143 in bovine muscle development remained unclear...
October 31, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27796640/erratum-to-reformation-of-tissue-balls-from-tentacle-explants-of-coral-goniopora-lobata-self-organization-process-and-response-to-environmental-stresses
#17
Qiongxuan Lu, Tao Liu, Xianming Tang, Bo Dong, Huarong Guo
No abstract text is available yet for this article.
October 28, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27778229/production-of-endothelial-progenitor-cells-from-skin-fibroblasts-by-direct-reprogramming-for-clinical-usages
#18
Phuc Van Pham, Ngoc Bich Vu, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, Ngoc Kim Phan
Endothelial progenitor cells (EPCs) play an important role in angiogenesis. However, they exist in limited numbers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F12 medium supplemented with 5% activated platelet-rich plasma and 1% antibiotic-antimycotic solution. They were then transfected with mRNA ETV2 and incubated in culture medium under hypoxia (5% oxygen) for 14 d...
October 24, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27778228/comparison-of-in-vitro-invasiveness-of-high-and-low-metastatic-triple-negative-human-breast-cancer-visualized-by-color-coded-imaging
#19
Shuya Yano, Kiyoto Takehara, Hiroshi Tazawa, Hiroyuki Kishimoto, Shunsuke Kagawa, Toshiyoshi Fujiwara, Robert M Hoffman
No abstract text is available yet for this article.
October 24, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27743338/erratum-to-dimethylfumarate-induces-cell-cycle-arrest-and-apoptosis-via-regulating-intracellular-redox-systems-in-hela-cells
#20
Guocan Han, Qiang Zhou
No abstract text is available yet for this article.
December 2016: In Vitro Cellular & Developmental Biology. Animal
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