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In Vitro Cellular & Developmental Biology. Animal

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https://www.readbyqxmd.com/read/28283876/purification-of-serine-protease-from-polychaeta-lumbrineris-nipponica-and-assessment-of-its-fibrinolytic-activity
#1
Seung Ju Yeon, Goo Yong Chung, Jae Sang Hong, Jin Ha Hwang, Hwa Sung Shin
Ischemic stroke and cardiovascular disease can occur from blockage of blood vessels by fibrin clots formed naturally in the body. Therapeutic drugs of anticoagulant or thrombolytic agents have been studied; however, various problems have been reported such as side effects and low efficacy. Thus, development of new candidates that are more effective and safe is necessary. The objective of this study is to evaluate fibrinolytic activity, anti-coagulation, and characterization of serine protease purified from Lumbrineris nipponica, polychaeta, for new thrombolytic agents...
March 10, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28233141/gfp-labeling-kinetics-of-triple-negative-human-breast-cancer-by-a-killer-reporter-adenovirus-in-3d-gelfoam%C3%A2-histoculture
#2
LETTER
Shuya Yano, Kiyoto Takehara, Shinji Miwa, Hiroyuki Kishimoto, Hiroshi Tazawa, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M Hoffman
No abstract text is available yet for this article.
February 23, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28229336/relevant-principal-factors-affecting-the-reproducibility-of-insect-primary-culture
#3
Norichika Ogata, Kikuo Iwabuchi
The primary culture of insect cells often suffers from problems with poor reproducibility in the quality of the final cell preparations. The cellular composition of the explants (cell number and cell types), surgical methods (surgical duration and surgical isolation), and physiological and genetic differences between donors may be critical factors affecting the reproducibility of culture. However, little is known about where biological variation (interindividual differences between donors) ends and technical variation (variance in replication of culture conditions) begins...
February 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205142/stepwise-morphological-changes-and-cytoskeletal-reorganization-of-human-mesenchymal-stem-cells-treated-by-short-time-cyclic-uniaxial-stretch
#4
Azim Parandakh, Mohammad Tafazzoli-Shadpour, Mohammad-Mehdi Khani
This study aimed to investigate stepwise remodeling of human mesenchymal stem cells (hMSCs) in response to cyclic stretch through rearrangement and alignment of cells and cytoskeleton regulation toward smooth muscle cell (SMC) fate in different time spans. Image analysis techniques were utilized to calculate morphological parameters. Cytoskeletal reorganization was observed by investigating F-actin filaments using immunofluorescence staining, and expression level of contractile SMC markers was followed by a quantitative polymerase chain reaction method...
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205141/cell-lines-derived-from-the-squash-bug-anasa-tristis-coreidae-hemiptera
#5
Cynthia L Goodman, Joseph A Ringbauer, Yao-Fa Li, Tamra Reall Lincoln, David Stanley
The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15)...
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205140/erratum-to-mesenchymal-stem-cells-attenuate-hydrogen-peroxide-induced-oxidative-stress-and-enhance-neuroprotective-effects-in-retinal-ganglion-cells
#6
Yi Cui, Nuo Xu, Wei Xu, Guoxing Xu
No abstract text is available yet for this article.
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28181104/apigenin-attenuates-streptozotocin-induced-pancreatic-%C3%AE-cell-damage-by-its-protective-effects-on-cellular-antioxidant-defense
#7
Ning Wang, Wen Jing Yi, Lu Tan, Jia Hui Zhang, Jiamin Xu, Yi Chen, Mengting Qin, Shuang Yu, Jing Guan, Rui Zhang
Pancreatic beta cells are very sensitive to oxidative stress, which is one of the major causes of cell damages in diabetes. Growing interest has focused on the development of effective therapeutics to protect pancreatic cells from oxidative stress and searching for potentially protective antioxidants for treating diabetes. Apigenin, a plant-derived flavonoid, was investigated to determine whether it could protect rat insulinoma cell lines (RINm5F pancreatic beta cells) against streptozotocin (STZ)-induced oxidative damages and the mechanisms implicated...
February 8, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28160207/impact-of-prolonged-oocyte-incubation-time-before-vitrification-on-oocyte-survival-embryo-formation-and-embryo-quality-in-mice
#8
Azade Karami, Mitra Bakhtiari, Mehri Azadbakht, Rostam Ghorbani, Mozafar Khazaei, Mansour Rezaei
Oocyte incubation time before freezing is one of the factors affecting oocyte vitrification. In the assisted reproductive technology (ART) clinics, it is sometimes decided to perform oocyte vitrification after a long period of incubation time due to various conditions, such as inability to collect semen samples, unsuccessful urological interventions (PESA, TESE, etc.), or unexpected conditions. A time factor of up to 6 h has been studied in the available reports. Therefore, this study was designed to evaluate oocyte incubation time before freezing at 0, 6, 12, 18, and 24 h after retrieval...
February 3, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28155130/rapid-isolation-of-murine-primary-hepatocytes-for-chromosomal-analysis
#9
Kentaro Ariyoshi, Yohei Fujishima, Tomisato Miura, Yi Shang, Shizuko Kakinuma, Shimada Yoshiya, Kosuke Kasai, Akifumi Nakata, Akira Tachibana, Mitsuaki A Yoshida
Primary hepatocyte culture is a crucial tool for investigations of liver function and for evaluating the toxic effects of drugs. In addition, chromosomal analysis of hepatocytes could also prove useful for understanding the mechanisms of hepatocarcinogenesis. However, cultivation of primary hepatocytes for chromosome analysis has been hampered by the specific equipment and skill required to perform the in situ perfusion step necessary for isolation of primary hepatocytes. In the present study, we aimed to establish a simple and efficient method of isolating hepatocytes suitable for chromosome analysis...
February 2, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28138860/preferential-pattern-of-mouse-neutrophil-cell-death-in-response-to-various-stimulants
#10
Nuttira Luehong, Juthamart Khaowmek, Kanruethai Wongsawan, Phongsakorn Chuammitri
Neutrophils undergo cell death processes once their physiological function has been fulfilled. Apoptosis, necrosis, pyroptosis, or NETosis, a unique form of cell death, could occur, depending on the type of stimulant or inhibitory intervention. We investigated whether phorbol myristate acetate (PMA) and Klebsiella pneumoniae (KP), serving as stimulants, or whether an inhibitor (cytochalasin B, CytB) could alter the morphology and gene expression pattern associated with mouse neutrophil cell death. Fluorescence microscopy, flow cytometry, and real-time PCR approaches were used to identify morphological changes, percentages of cell death, and gene expression patterns, respectively...
January 30, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28130754/characterization-of-smad3-knockout-mouse-derived-skin-cells
#11
Ke Liu, Zhen Gao, Guangdong Zhou, Wenjie Zhang, Xiaoli Wu, Wei Liu
TGF-β plays an important role in skin wound healing process, in which Smad3 acts as a signaling molecule. Smad3 knockout mice exhibit enhanced wound healing and less inflammatory process, but the intrinsic properties of the mouse derived skin cells are generally unexplored. The purpose of this study is to characterize the biological behavior of skin cells derived from Smad3 knockout mice and thus to define the mechanism of this particular wound healing process. Keratinocytes and dermal fibroblasts were harvested from the skin of Smad3 knockout (Smad3 KO) and wild-type (WT) mice and in vitro cultured for one and two passages for various experiments...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28130753/fluctuation-of-ros-regulates-proliferation-and-mediates-inhibition-of-migration-by-reducing-the-interaction-between-dlc1-and-cav-1-in-breast-cancer-cells
#12
Bingwu Yang, Wenzhen Zhu, Zhaodi Zheng, Rongfei Chai, Shuhua Ji, Guanghui Ren, Tingting Liu, Zhaojun Liu, Taiyu Song, Fenglin Li, Shan Liu, Guorong Li
The aim of our present study was to elucidate the effects of up-regulation and down-regulation of intracellular reactive oxygen species (ROS) level on proliferation, migration, and related molecular mechanism. Breast cancer cells were treated by catalase or H2O2. MTT, colony formation assay, and Hoechst/PI staining were used to evaluate proliferation and apoptosis. The level of intracellular ROS was measured by dichlorodihydrofluorescein diacetate probes. The ability of migration was detected by wound healing...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127704/effect-of-potential-role-of-p53-on-embryo-development-arrest-induced-by-h2o2-in-mouse
#13
De-Bao Hu, Zhong-Shu Li, Ihsan Ali, Li-Jie Xu, Nan-Zhu Fang
During mammalian embryo development in vitro, mechanism of embryonic development arrest caused by oxidative stress has not been clear so far. The tumor suppressor protein p53 controls cell cycle and programmed cell death by regulating relevant signal pathway. Recent researches revealed that the concentration and distribution of p53 are closely related with reactive oxygen species (ROS). The main objective of this experiment was to explore the role of p53 on embryonic development arrest caused by oxidative stress...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127703/are-your-results-valid-cellular-authentication-a-need-from-the-past-an-emergency-on-the-present
#14
Bruno Cosme, Priscila Falagan-Lotsch, Mariana Ribeiro, Kely Napoleão, José Mauro Granjeiro, Rodrigo Moura-Neto
The cultures of immortalized cells have been established in the 50s and become popular as a biological model for in vitro assays. The success and popularization brought side effects. Still, in the 60 years emerge the first cases of misidentification/contamination of cell line. Because of that, the scientific community has been oriented to authenticate their lines before performing assays. The use of cells with incorrect identification or contamination has been identified as responsible for an increasing number of unmatched results and a waste of resources...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127702/effect-of-sodium-butyrate-on-cell-proliferation-and-cell-cycle-in-porcine-intestinal-epithelial-ipec-j2-cells
#15
Yueqin Qiu, Xianyong Ma, Xuefen Yang, Li Wang, Zongyong Jiang
Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127701/high-metastatic-triple-negative-breast-cancer-variants-selected-in-vivo-become-chemoresistant-in-vitro
#16
LETTER
Shuya Yano, Kiyoto Takehara, Hiroyuki Kishimoto, Hiroshi Tazawa, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M Hoffman
No abstract text is available yet for this article.
January 26, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127700/identification-of-newly-established-spodoptera-littoralis-cell-lines-by-two-dna-barcoding-markers
#17
I Ahmed, H Huebner, Y I Mamoori, R Buchholz
Cell line authentication is crucial in determining the identity of cell lines and detecting any cross-contamination. The identity of three newly established Spodoptera littoralis cell lines (Spli-C, Spli-B, and Spli-S) was confirmed by DNA fingerprinting. In this study, we used two universal primers sets to amplify two DNA fragments in different positions in the mitochondrial cytochrome C oxidase 1 gene (COI). The PCR reaction succeeded in amplifying two target DNA amplicons. The first amplicon had ~650 bp, while the second had ~410 bp...
January 26, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28120247/heterogeneity-in-cancer-cells-variation-in-drug-response-in-different-primary-and-secondary-colorectal-cancer-cell-lines-in-vitro
#18
Melanie Arul, April Camilla Roslani, Swee Hung Cheah
Tumor heterogeneity may give rise to differential responses to chemotherapy drugs. Therefore, unraveling tumor heterogeneity has an implication for biomarker discovery and cancer therapeutics. To test this phenomenon, we investigated the differential responses of three secondary colorectal cancer cell lines of different origins (HCT116, HT29, and SW620 cells) and four novel primary cell lines obtained from different colorectal cancer patients to 5-fluorouracil (5-FU) and oxaliplatin (L-OHP) and explored the differences in gene expression among the primary cell lines in response to exposure to cytotoxic drugs...
January 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28078501/the-tissue-culture-laboratory-of-dr-george-otto-gey-60%C3%A2-yrs-ago-as-recalled-by-a-former-student
#19
Charles T Ambrose
George Otto Gey was a pioneer in tissue culture, having introduced the roller drum, the HeLa cell line, and the use of human fetal cord serum and beef embryo extract. During his career (1920s-1960s), the field of tissue culture was in its infancy and not yet dependent upon commercial biological supply houses. While the early techniques of cell culture have been greatly improved upon, of historical interest may be personal observations of the Gey Tissue Culture Laboratory, Johns Hopkins Medical School, as recalled by a medical student working there in the 1950s...
January 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28078500/effects-of-different-omega-3-sources-fish-oil-krill-oil-and-green-lipped-mussel-against-cytokine-mediated-canine-cartilage-degradation
#20
Kittisak Buddhachat, Puntita Siengdee, Siriwadee Chomdej, Kumpanart Soontornvipart, Korakot Nganvongpanit
Our purpose was to evaluate the protective effect of three marine omega-3 sources, fish oil (FO), krill oil (KO), and green-lipped mussel (GLM) against cartilage degradation. Canine cartilage explants were stimulated with either 10 ng/mL interleukin-1β (IL-1β) or IL-1β/oncostatin M (10 ng/mL each) and then treated with various concentrations of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA; 3 and 30 μg/mL), FO, KO, or GLM (250, 500, and 1000 μg/mL) for 28 days. Gene expression was then investigated in primary canine chondrocytes...
January 11, 2017: In Vitro Cellular & Developmental Biology. Animal
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