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In Vitro Cellular & Developmental Biology. Animal

Akhilendra Kumar Maurya, Manjula Vinayak
Flavonoids have been chronicles of the history of a long way journey in the cure of physiological or pathophysiological conditions in various diseases including cancer. Our previous findings suggest the extensive mechanism of quercetin (QUE) mediated regression of cell survival, cell proliferation, oxidative stress, inflammation, and angiogenesis via modulating PI3K and PKC signaling in lymphoma as well as hepatocellular carcinoma. PI3K-PKC pathway is a key monitor of mammalian cells regulated by its different isoenzymes, which may exert similar or opposite cellular effects by differential coupling of signaling pathways...
November 9, 2018: In Vitro Cellular & Developmental Biology. Animal
Rieko Oyama, Fusako Kito, Zhiwei Qiao, Marimu Sakumoto, Kumiko Shiozawa, Shunichi Toki, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Dermatofibrosarcoma protuberans (DFSP) is a common type of dermal sarcoma, characterized by the presence of the unique collagen type I alpha 1 chain (COL1A1)-PDGFB translocation, which causes constitutive activation of the platelet-derived growth factor β (PDGFB) signaling pathway. Patients with DFSP exhibit frequent local recurrence, and novel therapeutic approaches are required to achieve better clinical outcomes. Patient-derived cancer cell lines are essential in the preclinical research. Here, we established novel patient-derived DFSP cell lines from two patients with DFSP and designated these cell lines NCC-DFSP1-C1 and NCC-DFSP2-C1...
November 8, 2018: In Vitro Cellular & Developmental Biology. Animal
Kazuhiro Nagayama, Yoichi Miyamoto, Kotaro Kaneko, Kentaro Yoshimura, Kiyohito Sasa, Takaaki Akaike, Shigemoto Fujii, Eri Izumida, Risa Uyama, Daichi Chikazu, Koutaro Maki, Ryutaro Kamijo
Osteocytes regulate bone remodeling, especially in response to mechanical loading and unloading of bone, with nitric oxide reported to play an important role in that process. In the present study, we found that 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP), a second messenger of nitric oxide in various types of cells, was produced by osteocytes in bone tissue as well as cultured osteocytic Ocy454 cells. The amount of 8-nitro-cGMP in Ocy454 cells increased during incubation with parathyroid hormone or prostaglandin E2 , both of which are known to upregulate receptor activator of nuclear factor-κB ligand (RANKL) mRNA expression in osteocytes...
November 5, 2018: In Vitro Cellular & Developmental Biology. Animal
Kyeong Kweon, Jin-Ok Ahn, Woo-Jin Song, Qiang Li, Bo-Yeon Lee, Hyung-Kyu Chae, Hwa-Young Youn
The present study was designed to evaluate the effect of SB injection, which is composed of extracts from the roots of Pulsatilla koreana, Panax ginseng, and Glycyrrhiza glabra, on the viability of canine osteosarcoma and melanoma cells and nonneoplastic canine cells. Cells were treated with SB injection, conventional chemotherapeutic drugs, or a combination of both at various concentrations. Cellular viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry was used to evaluate the cell cycle and apoptosis...
October 31, 2018: In Vitro Cellular & Developmental Biology. Animal
Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
Monoclonal antibodies and antibody fragments are widely used in therapeutics and diagnoses. While mammalian cells serve as the host cells for antibody production, insect cells can produce large quantities of secretory antibodies in serum-free suspension cultures. The effects of lithium on the processes of autophagy and apoptosis in mammalian cells are well chronicled. In the present study, stably transformed insect cells, which produce an engineered antibody molecule, were cultured with lithium chloride in a serum-free medium...
October 31, 2018: In Vitro Cellular & Developmental Biology. Animal
Rongqun Li, Aiwen Zhuang, Jiawei Ma, Lina Ji, Xiaoli Hou, Hongbo Chen, Xiaoping Pan, Wenhong Liu
How genomic DNA methylation and methyl CpG-binding protein 2 (MeCP2) gene expression affect the pathogenesis of systemic lupus erythematosus (SLE) remains poorly understood. Traditional Chinese medicine has a unique effect in the treatment of SLE patients. This study aimed to investigate the effect of Jieduquyuziyin prescription (JP)-treated rat serum on the gene expression of MeCP2 in Jurkat T cells and its role in the pathogenesis of SLE. Jurkat T cells were harvested, and drug-containing serum was prepared...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Hongwei Zhang, Yaofa Li, Tamra Reall, Yiyun Xu, Cynthia Goodman, Stephen Saathoff, Joseph Ringbauer, David Stanley
Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Xiaolong Yuan, Xi Deng, Xiaofeng Zhou, Ailing Zhang, Yan Xing, Zhe Zhang, Hao Zhang, Jiaqi Li
In mammalian ovaries, many studies demonstrated that the proliferation and apoptosis of granulosa cells are involved in folliculogenesis. Previous evidence suggests that miR-126-3p might get involved in the proliferation and apoptosis of granulosa cells, and tuberous sclerosis complex 1 (TSC1) gene was predicted as one target of miR-126-3p, and moreover, granulosa cell-specific TSC1 knockout stimulated folliculogenesis in mice. However, the molecular regulation of miR-126-3p on TSC1 and its effects on cell proliferation and apoptosis remain virtually unexplored in granulosa cells...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Tianyu Yu, Bin Liu, Zhigang He, Muqing Yang, Jian Song, Cheng Ma, Sunqiang Ma, Junlan Feng, Xiaodong Wang, Jiyu Li
Mast cells (MCs) are responsible for the innate immune response. Rat MCs are more suitable than mouse MCs as models of specific parasite infection processes and ovalbumin-induced asthma. Rat peritoneum-derived MCs and RBL-2H3 cells (an MC cell line) are widely used in disease studies. However, the application of rat bone marrow-derived MCs (BMMCs) are poorly documented in terms of the methodology of rat BMMC isolation. Here, we describe a relatively rapid, efficient, and simple method for the cultivation of rat BMMCs...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Rieko Oyama, Fusako Kito, Zhiwei Qiao, Marimu Sakumoto, Rei Noguchi, Mami Takahashi, Shunichi Toki, Yoshikazu Tanzawa, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of genetic alterations in relevant cellular contexts. Here, we report the establishment and characterization of a novel Ewing's sarcoma cell line following primary Ewing's sarcoma tumor tissue culture. The established cell line was authenticated by DNA microsatellite short tandem repeat analysis, characterized by in vitro assays, and named NCC-ES1-C1...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Shiv Prakash Verma, Parimal Das
Monensin is a metal ionophore used as anticancer agent in many types of cancer cells. In this study, therapeutic potential of monensin was evaluated in TFE3 translocated renal cell carcinoma (RCC) cell line UOK146. UOK146 cells were treated with different concentrations of monensin, and cell death was induced as shown by MTT assay. Autophagy was studied by LC3 western, FACS and LC3 puncta formation after monensin treatment. Mitochondrial potential was studied by staining with TMRM and FACS. Antimetastatic potential of monensin was checked by inhibition of wound closure and MMP7 expression at RNA level...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Amirhossein Jahangiri, Maryam Dadmanesh, Khodayar Ghorban
Signal transducer and activator of transcription 3 (STAT3) interacts with many gene promoters and transcription factors such as hypoxia-induced factor 1α (HIF-1α). Recent evidences proposed that STAT3 and HIF-1α together are responsible for angiogenesis and immune response suppression. The main aim of this study was to inhibit STAT3 and HIF-1α and assess their effects on the expression of immunosuppressive cytokines. S31-201 and PX-478 were used to inhibit STAT3 and HIF-1α, respectively. In both hypoxic and normoxic conditions, intracellular levels of HIF-1α were evaluated by western blotting and flow cytometry...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Ji Min Lee, Byoung-Hee Lee, Seo-Na Chang, Hanseul Oh, Bokyeong Ryu, Ukjin Kim, Jae-Hak Park
It is important to secure various biological resources in situations of diminishing wildlife genetic diversity. Cultured cells are useful bioresources because they can stably store genetic information for a long time and can be expanded efficiently. Here, we established fibroblast cell lines from Apodemus agrarius as a new living resource. A. agrarius is an important sub-predator species in ecosystem food chains and for the study of infection epidemiology. Established cell lines were characterized by chromosome and mitochondrial gene analysis, the observation of cell morphology, and their anchorage-dependent growth pattern...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Verma Urja, Kashmira Khaire, Suresh Balakrishnan, Gowri Kumari Uggini
Chick embryonic cells can be used to develop an easy and economical in vitro model for conducting studies on the disease muscle dystrophy (MD). For this, the limb myoblasts from 11th day chick embryo were isolated and cultured. To this muscle cell culture, anti-dystroglycan antibody (IIH6) was added so as to target the α-dystroglycan and disrupt the connection between the cytoskeletal proteins and the extracellular matrix (which is a characteristic feature of MD). Cells were allowed to differentiate further and the morphometrics and mRNA expression were studied...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Marilu Constantino Max, Camila Bizarro-Silva, Isabela Búfalo, Suellen Miguez González, Andressa Guidugli Lindquist, Roberta Garbelini Gomes, Thales Ricardo Rigo Barreiros, Lívia Aires Lisboa, Fábio Morotti, Marcelo Marcondes Seneda
Folliculogenesis is a process of development and maturation of the ovarian follicles, being essential for the maintenance of fertility. In in vivo conditions, 99.9% of the follicles of an ovary do not ovulate and undergo atresia. In order to minimize this loss and to clarify the existing mechanisms, a technique was developed that allows for the in vitro follicular development. The objective of this study was to evaluate the effects of different epidermal growth factor (EGF) concentrations on the in vitro culturing of equine preantral follicles...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Robert J MacDonald, Andrew Yen
CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 mRNA is reportedly expressed in neutrophils following isolation, but its role in this cellular context is unknown. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it...
December 2018: In Vitro Cellular & Developmental Biology. Animal
Gulbahar Boyuk, A Arzu Yigit, Ilkay Aydogan
Islet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining glucose homeostasis. However, this treatment requires an extended period of immunosuppression to prevent rejection and recurrent transplantation to maintain function. Thus, to enhance the properties of transplanted islet cells, we examined the effect of the co-culture of luteal cells, which secrete progesterone, on islet cell viability, functionality, and revascularization. It was found that islet viability and functionality were higher in the co-cultured group than in single cultures of islets at 48 and 96 h, in parallel with increased progesterone and vascular endothelial growth factor (VEGF) secretion from luteal cells...
October 2018: In Vitro Cellular & Developmental Biology. Animal
Azim Parandakh, Mohammad Tafazzoli-Shadpour, Abdolreza Ardeshirylajimi, Arash Khojasteh, Mohammad-Mehdi Khani
Cellular mechanical characteristics represent cell ability to produce tissue-specific metabolites. Therefore, to achieve effective cell therapy, a better understanding of the effects of chemo-mechanical stimuli on the mechanical properties of in vitro-treated cells is essential. Herein, we investigated the effects of uniaxial strain on the mechanical properties of mesenchymal stem cells (MSCs) upon transforming growth factor beta 1 (TGF-β1 ) stimulation. The MSCs were categorized into control and test groups...
October 2018: In Vitro Cellular & Developmental Biology. Animal
Payal Agarwal, Patricia DeInnocentes, R Curtis Bird
p16 is an important tumor suppressor gene encoded by the INK4A/ARF/INK4B gene locus that is conserved in humans, rodents, and canids. p16 regulates cell cycle in early G1 phase inhibiting transition out of cell cycle from G1/S phase by regulating a multi-protein control complex. p16-associated proteins, cyclin D, CDK4, and CDK6, experience expression level decreases or do not change during cell differentiation and quiescence in contrast to constant p16 expression in post-proliferative cell phases. We hypothesized that p16 has alternate binding partners, other than classical proliferation-associated proteins such as CDKs, in these post-proliferative cell phases...
October 2018: In Vitro Cellular & Developmental Biology. Animal
Masamichi Hayashi, Naoki Yamamoto, Noriko Hiramatsu, Sumito Isogai, Yusuke Gotoh, Yasuhiro Goto, Masashi Kondo, Kazuyoshi Imaizumi
In recent research on regenerative medicine, three-dimensional (3D) tissue reconstruction using the induced pluripotent stem cell (iPS cell) differentiated cells has attracted attention. In this study, mouse lungs at 1.5, 10, and 20 d old were subjected to enzyme treatment, and aggregates formed in serum-free suspension culture (3D-culture) were observed. The number of aggregates formed was the highest in 1.5 d. The cell aggregates in which the interior of the aggregate is filled and form small vacuoles and the organoid-like aggregates having a relatively large vacuole inside and forming the alveolar-like structure were observed...
October 2018: In Vitro Cellular & Developmental Biology. Animal
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