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In Vitro Cellular & Developmental Biology. Animal

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https://www.readbyqxmd.com/read/28432599/the-beneficial-effect-of-repaglinide-on-in-vitro-maturation-and-development-ability-of-immature-mouse-oocytes
#1
Eshrat Kalehoei, Mehri Azadbakht
Repaglinide is a hypoglycemic drug, causing depolarization of the cell membrane, opening the voltage-gated calcium channels, and then increasing intracellular calcium in the pancreatic B cells by inhibition of the K-ATP-sensitive channels. Oocyte in vitro maturation (IVM) is influenced by different factors such as calcium signaling. In this study, we examined the effects of repaglinide on in vitro maturation and fertilization ability of mouse oocyte. Immature oocytes were isolated from female Naval Medical Research Institute mice which are 6-8 wk old mechanically and then cultured in 30 μl droplets of T6 medium with different concentrations of repaglinide...
April 21, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28432598/animal-posters
#2
(no author information available yet)
No abstract text is available yet for this article.
April 21, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28424975/etv-2-activated-proliferation-of-endothelial-cells-and-attenuated-acute-hindlimb-ischemia-in-mice
#3
Phuc Van Pham, Ngoc Bich Vu, Hoa Trong Nguyen, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, Oanh Thi-Kieu Nguyen, Ngoc Kim Phan
Ischemia is the reduction of blood flow to tissues by injury of blood vessels. Depending on the sites of tissues and grade of ischemia, ischemia can cause many serious complications. This study aimed to evaluate the effects of the E-twenty six (ETS) factor Ets variant 2 (ETV2) gene expression in angiogenesis and the effect of ETV2 gene therapy in a mouse model of hindlimb ischemia. The role of ETV2 on endothelial cell proliferation was evaluated in vitro. Knockdown of ETV2 expression was done using short hairpin RNA (shRNA) lentiviral viral particles...
April 19, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28374170/invitromatics-invitrome-and-invitroomics-introduction-of-three-new-terms-for-in-vitro-biology-and-illustration-of-their-use-with-the-cell-lines-from-rainbow-trout
#4
REVIEW
Niels C Bols, Phuc H Pham, Vivian R Dayeh, Lucy E J Lee
The literature on cell lines that have been developed from rainbow trout (RT) (Oncorhynchus mykiss) is reviewed to illustrate three new terms: invitromatics, invitrome, and invitroomics. Invitromatics is defined as the history, development, characterization, engineering, storage, and sharing of cell lines. RT invitromatics differs from invitromatics for humans and other mammals in several ways. Nearly all the RT cell lines have developed through spontaneous immortalization. No RT cell line undergoes senescence and can be described as being finite, whereas many human cell lines undergo senescence and are finite...
April 3, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28364348/analysis-of-soluble-factors-in-conditioned-media-derived-from-primary-cultures-of-cirrhotic-liver-of-biliary-atresia
#5
Taisuke Yamazaki, Mariko Wakai, Shin Enosawa, Takayoshi Tokiwa
Biliary atresia (BA) is a rare and serious liver disease in newborn infants. Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of BA may contain hepatic stem/progenitor cells in primary culture of NPC fractions. In this study, NPC fractions were subjected to primary or passage culture and found that clusters of hepatocyte-like cells appear even without adding hepatocyte growth factor (HGF) to the culture medium, but not in their passage culture used as a control. Based on these findings, conditioned media (CMs) were collected and soluble factors in the CMs were analyzed in order to elucidate the mechanism of the appearance of hepatocyte-like cells or their clusters...
March 31, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28364347/cyp1a1-based-on-metabolism-of-xenobiotics-by-cytochrome-p450-regulates-chicken-male-germ-cell-differentiation
#6
Dong Li, Man Wang, Shaoze Cheng, Chen Zhang, Yilin Wang, Wenhui Zhang, Ruifeng Zhao, Changhua Sun, Yani Zhang, Bichun Li
This study aimed to explore the regulatory mechanism of metabolism of xenobiotics by cytochrome P450 during the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs) and consummate the induction differentiation system of chicken embryonic stem cells (cESCs) into SSCs in vitro. We performed RNA-Seq in highly purified male ESCs, male primordial germ cells (PGCs), and SSCs that are associated with the male germ cell differentiation. Thereinto, the metabolism of xenobiotics by cytochrome P450 was selected and analyzed with Venny among male ESC vs male PGC, male PGC vs SSC, and male ESC vs SSC groups and several candidates differentially expressed genes (DEGs) were excavated...
March 31, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28342024/towards-optimization-of-odonto-osteogenic-bioengineering-in-vitro-comparison-of-simvastatin-sodium-fluoride-melanocyte-stimulating-hormone
#7
Vahid Zijah, Roya Salehi, Marziyeh Aghazadeh, Mohammad Samiei, Effat Alizadeh, Soodabeh Davaran
Tissue engineering has emerged as a potential therapeutic option for dental problems in recent years. One of the policies in tissue engineering is to use both scaffolds and additive factors for enhancing cell responses. This study aims to evaluate and compare the effect of three types of biofactors on poly-caprolactone-poly-ethylene glycol-poly caprolactone (PCL-PEG-PCL) nanofibrous scaffold on human dental pulp stem cell (hDPSCs) engineering. The PCL-PEG-PCL copolymer was synthesized with ring opening polymerization method, and its nanofiber scaffold was prepared by electrospinning method...
March 25, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28342023/biogenic-synthesis-of-marsilea-quadrifolia-gold-nanoparticles-a-study-of-improved-glucose-utilization-efficiency-on-3t3-l1-adipocytes
#8
Anindita Chowdhury, Selvaraj Kunjiappan, Chiranjib Bhattacharjee, Balasubramanian Somasundaram, Theivendren Panneerselvam
This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morphological characteristics were analyzed by XRD, TG-DTA, SEM-EDS, HRTEM, and SAED. The results of characterization studies were used to assess the properties of GNPs...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28342022/identifying-connexin-expression-and-determining-gap-junction-intercellular-communication-in-rainbow-trout-cells
#9
Joshua Hooper, Sarah J Poynter, Stephanie J DeWitte-Orr
Gap junctions are groups of membrane-bound channels that allow the passage of small molecules and ions between cells, permitting cell-cell communication. Because of their importance in cell homeostasis, gap junction presence and function were characterized in three commonly studied rainbow trout cell lines, namely RTgill-W1, RTgutGC, and RTG-2. Firstly, gap junction presence was determined by screening for gap junction protein alpha 7 and alpha 1 (GJA7 and GJA1) presence at the transcript level and GJA7 at the protein level...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28342021/establishment-of-immortalized-mouse-intestinal-epithelial-cells-line-and-study-of-effects-of-arg-arg-on-inflammatory-response
#10
Kang Zhan, Maocheng Jiang, Yannan Sui, Kang Yan, Miao Lin, Guoqi Zhao
Primary mouse intestinal epithelial cells (MIEs) are not ideal models for long-term culture in vitro and a limited amount of approximate three generations. In addition, the mechanism that arginine-arginine dipeptide (Arg-Arg) regulates mouse intestinal inflammatory response remains unknown. Therefore, the aim of this study was to establish immortal MIEs and study the effects of Arg-Arg on inflammatory response after challenging the MIEs with lipopolysaccharide (LPS) or staphylococcal enterotoxin C (rSEC). Our data showed that immortalized MIEs could be cultured over 100 generations...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28283876/purification-of-serine-protease-from-polychaeta-lumbrineris-nipponica-and-assessment-of-its-fibrinolytic-activity
#11
Seung Ju Yeon, Goo Yong Chung, Jae Sang Hong, Jin Ha Hwang, Hwa Sung Shin
Ischemic stroke and cardiovascular disease can occur from blockage of blood vessels by fibrin clots formed naturally in the body. Therapeutic drugs of anticoagulant or thrombolytic agents have been studied; however, various problems have been reported such as side effects and low efficacy. Thus, development of new candidates that are more effective and safe is necessary. The objective of this study is to evaluate fibrinolytic activity, anti-coagulation, and characterization of serine protease purified from Lumbrineris nipponica, polychaeta, for new thrombolytic agents...
March 10, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28233141/gfp-labeling-kinetics-of-triple-negative-human-breast-cancer-by-a-killer-reporter-adenovirus-in-3d-gelfoam%C3%A2-histoculture
#12
LETTER
Shuya Yano, Kiyoto Takehara, Shinji Miwa, Hiroyuki Kishimoto, Hiroshi Tazawa, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M Hoffman
No abstract text is available yet for this article.
February 23, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28229336/relevant-principal-factors-affecting-the-reproducibility-of-insect-primary-culture
#13
Norichika Ogata, Kikuo Iwabuchi
The primary culture of insect cells often suffers from problems with poor reproducibility in the quality of the final cell preparations. The cellular composition of the explants (cell number and cell types), surgical methods (surgical duration and surgical isolation), and physiological and genetic differences between donors may be critical factors affecting the reproducibility of culture. However, little is known about where biological variation (interindividual differences between donors) ends and technical variation (variance in replication of culture conditions) begins...
February 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205142/stepwise-morphological-changes-and-cytoskeletal-reorganization-of-human-mesenchymal-stem-cells-treated-by-short-time-cyclic-uniaxial-stretch
#14
Azim Parandakh, Mohammad Tafazzoli-Shadpour, Mohammad-Mehdi Khani
This study aimed to investigate stepwise remodeling of human mesenchymal stem cells (hMSCs) in response to cyclic stretch through rearrangement and alignment of cells and cytoskeleton regulation toward smooth muscle cell (SMC) fate in different time spans. Image analysis techniques were utilized to calculate morphological parameters. Cytoskeletal reorganization was observed by investigating F-actin filaments using immunofluorescence staining, and expression level of contractile SMC markers was followed by a quantitative polymerase chain reaction method...
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205141/cell-lines-derived-from-the-squash-bug-anasa-tristis-coreidae-hemiptera
#15
Cynthia L Goodman, Joseph A Ringbauer, Yao-Fa Li, Tamra Reall Lincoln, David Stanley
The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15)...
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28205140/erratum-to-mesenchymal-stem-cells-attenuate-hydrogen-peroxide-induced-oxidative-stress-and-enhance-neuroprotective-effects-in-retinal-ganglion-cells
#16
Yi Cui, Nuo Xu, Wei Xu, Guoxing Xu
No abstract text is available yet for this article.
February 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28181104/apigenin-attenuates-streptozotocin-induced-pancreatic-%C3%AE-cell-damage-by-its-protective-effects-on-cellular-antioxidant-defense
#17
Ning Wang, Wen Jing Yi, Lu Tan, Jia Hui Zhang, Jiamin Xu, Yi Chen, Mengting Qin, Shuang Yu, Jing Guan, Rui Zhang
Pancreatic beta cells are very sensitive to oxidative stress, which is one of the major causes of cell damages in diabetes. Growing interest has focused on the development of effective therapeutics to protect pancreatic cells from oxidative stress and searching for potentially protective antioxidants for treating diabetes. Apigenin, a plant-derived flavonoid, was investigated to determine whether it could protect rat insulinoma cell lines (RINm5F pancreatic beta cells) against streptozotocin (STZ)-induced oxidative damages and the mechanisms implicated...
February 8, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28160207/impact-of-prolonged-oocyte-incubation-time-before-vitrification-on-oocyte-survival-embryo-formation-and-embryo-quality-in-mice
#18
Azade Karami, Mitra Bakhtiari, Mehri Azadbakht, Rostam Ghorbani, Mozafar Khazaei, Mansour Rezaei
Oocyte incubation time before freezing is one of the factors affecting oocyte vitrification. In the assisted reproductive technology (ART) clinics, it is sometimes decided to perform oocyte vitrification after a long period of incubation time due to various conditions, such as inability to collect semen samples, unsuccessful urological interventions (PESA, TESE, etc.), or unexpected conditions. A time factor of up to 6 h has been studied in the available reports. Therefore, this study was designed to evaluate oocyte incubation time before freezing at 0, 6, 12, 18, and 24 h after retrieval...
February 3, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28155130/rapid-isolation-of-murine-primary-hepatocytes-for-chromosomal-analysis
#19
Kentaro Ariyoshi, Yohei Fujishima, Tomisato Miura, Yi Shang, Shizuko Kakinuma, Shimada Yoshiya, Kosuke Kasai, Akifumi Nakata, Akira Tachibana, Mitsuaki A Yoshida
Primary hepatocyte culture is a crucial tool for investigations of liver function and for evaluating the toxic effects of drugs. In addition, chromosomal analysis of hepatocytes could also prove useful for understanding the mechanisms of hepatocarcinogenesis. However, cultivation of primary hepatocytes for chromosome analysis has been hampered by the specific equipment and skill required to perform the in situ perfusion step necessary for isolation of primary hepatocytes. In the present study, we aimed to establish a simple and efficient method of isolating hepatocytes suitable for chromosome analysis...
February 2, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28138860/preferential-pattern-of-mouse-neutrophil-cell-death-in-response-to-various-stimulants
#20
Nuttira Luehong, Juthamart Khaowmek, Kanruethai Wongsawan, Phongsakorn Chuammitri
Neutrophils undergo cell death processes once their physiological function has been fulfilled. Apoptosis, necrosis, pyroptosis, or NETosis, a unique form of cell death, could occur, depending on the type of stimulant or inhibitory intervention. We investigated whether phorbol myristate acetate (PMA) and Klebsiella pneumoniae (KP), serving as stimulants, or whether an inhibitor (cytochalasin B, CytB) could alter the morphology and gene expression pattern associated with mouse neutrophil cell death. Fluorescence microscopy, flow cytometry, and real-time PCR approaches were used to identify morphological changes, percentages of cell death, and gene expression patterns, respectively...
January 30, 2017: In Vitro Cellular & Developmental Biology. Animal
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