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In Vitro Cellular & Developmental Biology. Animal

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https://www.readbyqxmd.com/read/28808859/best-practices-in-cell-culture-an-overview
#1
John M Baust, Gertrude Case Buehring, Lia Campbell, Eugene Elmore, John W Harbell, Raymond W Nims, Paul Price, Yvonne A Reid, Frank Simione
This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells...
August 14, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28733930/best-practices-for-the-use-and-evaluation-of-animal-serum-as-a-component-of-cell-culture-medium
#2
Raymond W Nims, John W Harbell
Animal serum is a common additive for cell culture medium and is often required at 5 to 10% (v/v) for the attachment and growth of primary and continuous anchorage-dependent (monolayer) cultures. The use of animal serum in cell culture medium confers several advantages and also some risks. This article discusses the use of animal serum as a component of cell culture medium. The best practices associated with the sourcing, storage, thawing, testing, and mitigation of risk associated with the use of animal sera are among the topics described in this article...
July 21, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28726188/a-lncrna-promotes-myoblast-proliferation-by-up-regulating-gh1
#3
Yingwei Yue, Congfei Jin, Mingming Chen, Linlin Zhang, Xinfeng Liu, Wenzhi Ma, Hong Guo
Long noncoding RNAs (lncRNAs) are key regulatory factors for gene expression in a variety of biological processes; however, the role of lncRNAs in muscle formation and development is poorly understood, particularly in cattle. Here, we identified a highly expressed lncRNA in muscle, lncYYW, by high-throughput sequencing in bovine longissimus, scapular, intercostal, and gluteus muscles. The expression of lncYYW increased gradually during myoblast differentiation. Overexpression of lncYYW increased the number of cells in the DNA synthesis (S) stage of the cell cycle and upregulated the expression of two well-established myogenic markers, myogenin and myosin heavy chain...
July 19, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28726187/best-practices-for-media-selection-for-mammalian-cells
#4
REVIEW
Paul J Price
Cell culture medium is a complex mixture of nutrients and growth factors that, along with the physical environment, can either help or destroy your experiment or production run. Nutritional requirements differ with different cell types and functions, as do optimal pH and osmolality. As cell growth proceeds, different cells will utilize amino acids and other components at different rates. By controlling for ammonia, free radicals, heavy metal toxicity, pH shifts, fluctuations in osmolality, nutrient depletion, and chemical and biological contaminants, you will optimize the chances of success...
July 19, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28726186/erratum-to-prostaglandin-actions-in-established-insect-cell-lines
#5
Yao-Fa Li, Hongwei Zhang, Joseph A Ringbauer, Cynthia L Goodman, Tamra Reall Lincoln, Kaile Zhou, David Stanley
No abstract text is available yet for this article.
July 19, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28710602/eldecalcitol-ed-71-an-analog-of-1%C3%AE-25-oh-2d3-inhibits-the-growth-of-squamous-cell-carcinoma-scc-cells-in-vitro-and-in-vivo-by-down-regulating-expression-of-heparin-binding-protein-17-fibroblast-growth-factor-binding-protein-1-hbp17-fgfbp-1-and-fgf-2
#6
T Shintani, F Takatsu, S N Z Rosli, E Usui, A Hamada, K Sumi, Y Hayashido, S Toratani, Tetsuji Okamoto
Heparin-binding protein 17 (HBp17)/fibroblast growth factor-binding protein-1 (FGFBP-1) was first purified from medium conditioned by A431 cells for its capacity to bind to fibroblast growth factors 1 and 2 (FGF-1 and -2). Among FGF family members, FGF-2 is a potent mitogen for various cell types, including vascular endothelial cells, fibroblasts, and cancer cells such as oral squamous cell carcinoma (OSCC) cells. Besides being well known in bone metabolism, the active form of vitamin D3, i.e., 1α,25(OH)2D3 (1,25D3), was reported to have protective effects for heart disease and cancer...
July 14, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28707225/establishment-and-characterization-of-a-brain-cell-line-from-sea-perch-lateolabrax-japonicus
#7
Yao Le, Yunlong Li, Yilin Jin, Peng Jia, Kuntong Jia, Meisheng Yi
A continuous cell line, designated LJB, derived from the brain of sea perch (Lateolabrax japonicus) was established. LJB cells have been subcultured for more than 60 times in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum (FBS) since the initial primary culture. LJB cells exhibited maximum growth rate at 28°C in DMEM supplemented with 20% FBS. Cytogenetic analysis indicated that the modal chromosome number was 48, which was identical with the chromosome number of embryonic stem-like cells of sea perch...
July 13, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28707224/the-influence-of-simulated-microgravity-on-proliferation-and-apoptosis-in-u251-glioma-cells
#8
Jiao Zhao, He Ma, Leitao Wu, Liang Cao, Qianqian Yang, Haijun Dong, Zongren Wang, Jing Ma, Zhen Li
Several studies have indicated that microgravity can influence cellular progression, proliferation, and apoptosis in tumor cell lines. In this study, we observed that simulated microgravity inhibited proliferation and induced apoptosis in U251 malignant glioma (U251MG) cells. Furthermore, expression of the apoptosis-associated proteins, p21 and insulin-like growth factor binding protein-2 (IGFBP-2), was upregulated and downregulated, respectively, following exposure to simulated microgravity. These findings indicate that simulated microgravity inhibits proliferation while inducing apoptosis of U251MG cells...
July 13, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28707223/inhibition-of-exendin-4-induced-steatosis-by-protein-kinase-a-in-cultured-hepg2-human-hepatoma-cells
#9
Alice Y Chen-Liaw, Gabrielle Hammel, George Gomez
Nonalcoholic fatty liver is characterized by the abnormal accumulation of triglycerides within hepatocytes, resulting in a steatotic liver. Glucagon-like peptide 1 and its analog exendin-4 can ameliorate certain aspects of this syndrome by inducing weight loss and reducing hepatic triglyceride accumulation, but it is unclear whether these effects result from the effects of glucagon-like peptide 1 on the pancreas, or from direct action on the liver. This study investigated the direct action and putative cellular mechanism of exendin-4 on steatotic hepatocytes in culture...
July 13, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28702926/the-stability-evaluation-of-mesenchymal-stem-cells-differentiation-toward-endothelial-cells-by-chemical-and-mechanical-stimulation
#10
Elham Hasanzadeh, Ghassem Amoabediny, Nooshin Haghighipour, Nasim Gholami, Javad Mohammadnejad, Shahrokh Shojaei, Nasim Salehi-Nik
Adipose-derived mesenchymal stem cells (ADSCs) are adult multipotent cells able to differentiate into several cell lineages. Vascular endothelial growth factor (VEGF) and the shear stress associated with blood flow are considered as the most important chemical and mechanical cues that play major roles in endothelial differentiation. However, the stability of endothelial-specific gene expression has not been completely addressed yet. ADSCs in passage 3 were cultured inside the tubular silicon tubes and then exposed to VEGF or shear stress produced in a perfusion bioreactor...
July 12, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699140/effects-of-different-sertoli-cell-types-on-the-maintenance-of-adult-spermatogonial-stem-cells-in-vitro
#11
Maryam Baazm, Farideh Jalali Mashayekhi, Saeid Babaie, Parvindokht Bayat, Cordian Beyer, Adib Zendedel
Spermatongonial stem cells (SSCs) are unique testis cells that are able to proliferate, differentiate, and transmit genetic information to the next generation. However, the effect of different Sertoli cell types on the expression of specific SSC genes is not yet well understood. In this study, we compare the in vitro effect of adult Sertoli cells, embryonic Sertoli cells, and TM4 (a Sertoli cell line) as feeder layers on the expression of SSC genes. SSCs were isolated from the testis of adult male mice and purified by differential plating...
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699139/response-to-comments-on-effects-of-different-omega-3-sources-fish-oil-krill-oil-and-green-lipped-mussel-against-cytokine-mediated-canine-cartilage-degradation-in-vitro-cell-dev-biol-anim-2017-doi-10-1007-s11626-016-0125-y
#12
LETTER
Kittisak Buddhachat, Puntita Siengdee, Siriwadee Chomdej, Kumpanart Soontornvipart, Korakot Nganvongpanit
No abstract text is available yet for this article.
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699138/toxicity-membrane-binding-and-uptake-of-the-sclerotinia-sclerotiorum-agglutinin-ssa-in-different-insect-cell-lines
#13
Ying Shen, Kristof De Schutter, Tomasz Walski, Els J M Van Damme, Guy Smagghe
The fungal lectin purified from Sclerotinia sclerotiorum, further referred to as Sclerotinia sclerotiorum agglutinin or SSA, possesses insecticidal activity against important pest insects such as pea aphids (Acyrthosiphon pisum). This paper aims at a better understanding of its activity at cellular level. Therefore, different insect cell lines were treated with SSA. These cell lines were derived from different tissues and represent the three major orders of insects important in agriculture: CF-203 (midgut Choristoneura fumiferana, Lepidoptera), GUTAW1 (midgut, Helicoverpa zea, Lepidoptera), High5 cells (ovary, Trichoplusia ni, Lepidoptera), Sf9 (ovary cells from Spodoptera frugiperda, Lepidoptera), S2 (hemocyte, Drosophila melanogaster, Diptera), and TcA (whole body, Tribolium castaneum, Coleoptera)...
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28699137/erratum-to-hepatopancreatic-cells-of-a-stone-crab-menippe-frontalis-from-per%C3%A3%C2%BA-separation-viability-study-and-evaluation-of-lipoperoxidation-against-cadmium-contamination
#14
H A Vitorino, R Y P Alta, E C P Alta, P Ortega
No abstract text is available yet for this article.
July 11, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28646290/2017-in-vitro-biology-meeting-june-10-14-raleigh-north-carolina-late-submission-abstracts
#15
(no author information available yet)
No abstract text is available yet for this article.
June 23, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28643224/establishment-of-a-3d-cell-culture-model-of-primary-bovine-mammary-epithelial-cells-extracted-from-fresh-milk
#16
Maria Hillreiner, Nadine I Müller, Heiner M Koch, Christiane Schmautz, Bernhard Küster, Michael W Pfaffl, Heike Kliem
For the investigation of molecular processes underlying diseases of the bovine mammary gland, primary bovine mammary epithelial cells (pbMEC) are used. They are known to contribute to the innate immune system of the bovine mammary gland. The functionality of pbMEC depends on the maintenance of in vivo characteristics. So far, the optimization of pbMEC culture conditions was intended in a variety of experiments. For this purpose, most of the studies used stable cell lines or primary cells obtained from udder biopsies of slaughtered animals...
June 22, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28597334/jak-stat-signaling-regulation-of-chicken-embryonic-stem-cell-differentiation-into-male-germ-cells
#17
Yu Zhang, Lei Zhang, Qisheng Zuo, Yinjie Wang, Yani Zhang, Qi Xu, Bichun Li, Guohong Chen
The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling is crucial in chicken germ stem cell differentiation, but its role in the regulation of germ cell differentiation is unknown. To address this, cucurbitacin I or interleukin 6 was used to inhibit or activate JAK-STAT signaling during embryonic stem cells (ESCs) differentiation. The expression of downstream JAK-STAT signaling molecules was assessed by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR)...
June 8, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28593424/hepatopancreatic-cells-of-a-stone-crab-menippe-frontalis-from-per%C3%A3%C2%BA-separation-viability-study-and-evaluation-of-lipoperoxidation-against-cadmium-contamination
#18
H A Vitorino, R Y A Pastrana, E C A Pastrana, P Ortega
Crustaceans are frequently used as bioindicators, and changes in their metabolism at the hepatopancreas (HP) level are often followed in these studies. The HP is the site of digestion, absorption, nutrient storage, and toxic metal detoxification, enabling crabs to survive in metal contaminated regions. Cellular damage and high lipid peroxidation (LPO) levels have been found in crab populations under high cadmium (Cd) concentrations. The aim of this work was to separate and characterize the HP cells of the stone crab Menippe frontalis from the Pacific Ocean, Perú (5° 5' 21″ S-81° 6' 51″ W) and to measure the cellular viability and LPO after exposure to the non-essential metal Cd...
June 7, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28550621/education-silent-abstracts
#19
(no author information available yet)
No abstract text is available yet for this article.
May 26, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28550620/plant-posters
#20
(no author information available yet)
No abstract text is available yet for this article.
May 26, 2017: In Vitro Cellular & Developmental Biology. Animal
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