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In Vitro Cellular & Developmental Biology. Animal

Hao Xu, Na Wang, Wenyue Cao, Liang Huang, Jianfeng Zhou, Lingshuang Sheng
Nowadays, adoptive T cell immunotherapy is emerging as a novel and potent treatment for cancer. To prepare enough effective T cells for treatment use, their rapid expansion is favorable. Our study compared 6 commonly used cultural media for human T cells, including serum-containing media and serum-free media, namely RPMI 1640, IMDM, Gibco OpTmizer CTS T Cell Expansion SFM, Gibco AIM-V Medium CTS, LONZA X-VIVO 15, and StemSpan SFEM with or without Dynabeads Human T-Activator CD3/CD28, on in vitro T cell expansion, apoptosis, and immune phenotype...
July 12, 2018: In Vitro Cellular & Developmental Biology. Animal
Minh Nguyen Tuyet Le, Mika Takahi, Kenshiro Maruyama, Akira Kurisaki, Kiyoshi Ohnuma
A high density of human-induced pluripotent stem cells (hiPSCs) improves the efficiency of cardiac differentiation, suggesting the existence of indispensable cell-cell interaction signals. The complexity of interactions among cells at high density hinders the understanding of the roles of cell signals. In this study, we determined the minimum cell density that can initiate differentiation to facilitate cell-cell interaction studies. First, we co-induced cardiac differentiation in the presence of the glycogen synthase kinase-3β inhibitor CHIR99021 and activin A at various cell densities...
July 2, 2018: In Vitro Cellular & Developmental Biology. Animal
Fusako Kito, Rieko Oyama, Marimu Sakumoto, Mami Takahashi, Kumiko Shiozawa, Zhiwei Qiao, Hiromi Sakamoto, Takeshi Hirose, Nokitaka Setsu, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes...
June 25, 2018: In Vitro Cellular & Developmental Biology. Animal
Xinxin Li, Xudong Ao, Li Bai, Dongfang Li, Xuefei Liu, Zhuying Wei, Shorgan Bou, Guangpeng Li
SCNT technology has been successfully used to clone a variety of mammals, but the cloning efficiency is very low. This low efficiency is likely due to the incomplete reprogramming of SCNT embryos. Histone modification and DNA methylation may participate in these events. Thus, it would be interesting to attempt to improve the efficiency of SCNT by using a HDACi VPA. In order to guarantee the effect of VPA and reduce its cytotoxicity, a comprehensive analysis of the cell proliferation and histone modification was performed...
June 25, 2018: In Vitro Cellular & Developmental Biology. Animal
(no author information available yet)
No abstract text is available yet for this article.
June 19, 2018: In Vitro Cellular & Developmental Biology. Animal
Luiza Bento de Queiroz Neta, Gabriela Pereira de Oliveira Lira, Alana Azevedo Borges, Maria Valéria de Oliveira Santos, Maria Bárbara Silva, Lhara Ricarliany Medeiros de Oliveira, Alexandre Rodrigues Silva, Moacir Franco de Oliveira, Alexsandra Fernandes Pereira
Animal cloning is a promising technology for biodiversity conservation, and its success depends on the recovery of nucleus donor cells. Specifically for collared peccaries, found sometimes in regions that are difficult to access, the storage at 4-6°C of skin tissues would be an alternative for the conservation of genetic material. Therefore, we aimed to evaluate different storage periods and the presence of a nutrient medium at 4-6°C on the recovery of somatic cells from the skin of collared peccaries. To analyze cell recovery rates, ear explants were distributed in non-refrigerated samples and samples refrigerated for 10, 30, and 50 d in the absence or presence of nutrient medium...
June 19, 2018: In Vitro Cellular & Developmental Biology. Animal
Vidya Jayasankar, Srinivasa Raghavan Vasudevan, Suja C Poulose, Indira Divipala
Mantle tissue from the black-lip pearl oyster, Pinctada margaritifera, was cultured in vitro using sterilized seawater supplemented with 0.1% yeast extract as the culture medium. Granular and agranular epithelial cells, hyalinocytes, and fibroblast-like cells were observed in the initial stages of culture. Epithelial cells later formed pseudopodial cell networks containing clusters of granulated cells, which upon maturation released their colored granules. These granules induced formation of nacre crystal deposits on the bottom of the culture plate...
June 12, 2018: In Vitro Cellular & Developmental Biology. Animal
Michihiro Toritsuka, Manabu Makinodan, Takahira Yamauchi, Yasunori Yamashita, Daisuke Ikawa, Takashi Komori, Sohei Kimoto, Kaori Hamano-Iwasa, Hideo Matsuzaki, Toshifumi Kishimoto
Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable...
June 12, 2018: In Vitro Cellular & Developmental Biology. Animal
Jooyeon Lee, Jeong-Ran Park, Hanbyeol Lee, Soojin Jang, Se-Min Ryu, Hyejin Kim, Donguk Kim, Aera Jang, Se-Ran Yang
L-carnosine, a dipeptide of the amino acids β-alanine and histidine, is found in various tissues, such as the central nervous system and skeletal muscles. Recently, L-carnosine has been reported to possess anti-tumor activity; however, the molecular mechanism underlying its activity in colorectal cancer is still unknown. Therefore, we investigated the effect of L-carnosine using a human colorectal cancer cell line, HCT116. Treatment with L-carnosine (0, 100, or 200 mM) for 24 h gradually reduced cellular proliferation according to immunochemistry and 7-aminoactinomycin D (7-AAD) analyses and induced G0/G1 phase arrest...
June 4, 2018: In Vitro Cellular & Developmental Biology. Animal
Kan Kato, Koji Morita, Isao Hirata, Kazuya Doi, Takayasu Kubo, Koichi Kato, Kazuhiro Tsuga
Inorganic polyphosphate has been expected to accelerate bone regeneration. However, there are limited evidences to prove that polyphosphate adsorbed on the surface of a hydroxyapatite plate enhances calcification of cultured osteoblasts. In this study, we examined the effect of polyphosphate adsorbed onto the surface of a hydroxyapatite plate on the attachment, proliferation, differentiation, and calcification of osteoblasts. After hydroxyapatite plates were soaked in solutions of polyphosphate, the plate surfaces were analyzed by scanning electron microscopy and toluidine blue staining to confirm adsorption of polyphosphate...
May 15, 2018: In Vitro Cellular & Developmental Biology. Animal
Baojin Yao, Mei Zhang, Xiangyang Leng, Meixin Liu, Yuxin Liu, Yaozhong Hu, Daqing Zhao, Yu Zhao
The Sika deer antler is well known for its unique ability to regenerate repeatedly and grow rapidly. Furthermore, it is a precious traditional Chinese medicine and has been widely used for more than 20 centuries. The major bioactive components within the antlers are water-soluble proteins, polypeptides, and free amino acids. Many studies have shown that water-soluble antler extracts play pivotal roles in wound healing, immune system modulation, anti-oxidation, and anti-inflammation. However, the exact effects on chondrocytes are still largely unknown...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Rieko Oyama, Mami Takahashi, Fusako Kito, Marimu Sakumoto, Kumiko Shiozawa, Zhiwei Qiao, Akihiko Yoshida, Makoto Endo, Akira Kawai, Tadashi Kondo
Primary leiomyosarcoma (LMS) of bone is a rare and aggressive mesenchymal malignancy that differentiates toward smooth muscle. Complete resection is the only curable treatment, and novel therapeutic approaches for primary LMS of bone have long been desired. Patient-derived xenografts (PDXs) and cell lines are invaluable tools for preclinical studies. Here, we established PDXs from a patient with primary LMS of bone and a cell line from an established PDX. Bone primary LMS tissue was subcutaneously implanted into highly immune-deficient mice...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Yang Wang, Meiling Zhang, Zi-Jiang Chen, Yanzhi Du
Vitrification of oocytes is closely associated with the lower embryonic developmental potential, which involves the cryopreservation injury occurred during vitrification. It indicates that vitrification may need to be further optimized. Therefore, we studied the effects of resveratrol, an antioxidant, on the developmental potential of vitrified mouse oocytes after in vitro fertilization. After adding a series of concentrations of resveratrol (0, 1, 10, 25, and 50 μM) into vitrification, warming, and post-warming mediums, we found that 25 and 50 μM resveratrol increased the blastocyst formation rate of vitrified oocytes...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Yusuke Aida, Hidemi Kurihara, Koichi Kato
Cementum is a calcified, avascular connective tissue that laminates the root of a tooth and plays a pivotal role in the development, homeostasis, and regeneration of a periodontal tissue. As a potential treatment for periodontal tissue defects in the patient with chronic periodontitis, much attention has been paid to tissue engineering combined with mesenchymal stem cells for regenerating periodontal tissues including cementum. However, limited information is available for the molecular factors that have impacts on the differentiation of mesenchymal stem cells into cementoblasts...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Claudia Miersch, Katja Stange, Monika Röntgen
Muscle stem cells, termed satellite cells (SC), and SC-derived myogenic progenitor cells (MPC) are involved in postnatal muscle growth, regeneration, and muscle adaptability. They can be released from their natural environment by mechanical disruption and tissue digestion. The literature contains several isolation protocols for porcine SC/MPC including various digestion procedures, but comparative studies are missing. In this report, classic trypsinization and a more complex trypsin, collagenase, and DNase (TCD) digestion were performed with skeletal muscle tissue from 4- to 5-d-old piglets...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Sang June Park, Hyungtaek Jeon, Seung-Min Yoo, Myung-Shin Lee
Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Taisuke Yamazaki, Shin Enosawa, Takayoshi Tokiwa
Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of biliary atresia (BA) may contain stem/progenitor cells, and clusters of hepatocyte-like cells appear via hepatocyte growth factor/c-Met signaling in primary cultures of NPCs. BA is a rare and serious liver disease, and procurement of BA cells is difficult. Therefore, cryopreservation of BA liver cells is an unavoidable challenge. In this study, we examined the appearance and liver function of hepatocyte-like cells in cultures of BA liver-derived NPC fractions after cryopreservation for 1 or 6 mo using a chemically defined cryopreservation solution, STEM-CELLBANKER...
June 2018: In Vitro Cellular & Developmental Biology. Animal
Atsuko Miyazawa, Shiuhyang Kuo, Stephen E Feinberg
Primary keratinocytes derived from human epidermis are widely used in tissue engineering and regenerative medicine. An important aspect in clinical applications is the preservation of human skin keratinocyte stem cells. However, it is difficult to expand the number of human skin keratinocyte stem cells, which are undifferentiated and highly proliferative in culture by using standard cell culture methods. It is even more difficult to identify them, since universal specific markers for human skin keratinocyte stem cells have not been identified...
June 2018: In Vitro Cellular & Developmental Biology. Animal
(no author information available yet)
No abstract text is available yet for this article.
June 2018: In Vitro Cellular & Developmental Biology. Animal
(no author information available yet)
No abstract text is available yet for this article.
June 2018: In Vitro Cellular & Developmental Biology. Animal
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