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In Vitro Cellular & Developmental Biology. Animal

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https://www.readbyqxmd.com/read/29785536/wnt3a-promotes-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells-into-cementoblast-like-cells
#1
Yusuke Aida, Hidemi Kurihara, Koichi Kato
Cementum is a calcified, avascular connective tissue that laminates the root of a tooth and plays a pivotal role in the development, homeostasis, and regeneration of a periodontal tissue. As a potential treatment for periodontal tissue defects in the patient with chronic periodontitis, much attention has been paid to tissue engineering combined with mesenchymal stem cells for regenerating periodontal tissues including cementum. However, limited information is available for the molecular factors that have impacts on the differentiation of mesenchymal stem cells into cementoblasts...
May 21, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29785535/effects-of-trypsinization-and-of-a-combined-trypsin-collagenase-and-dnase-digestion-on-liberation-and-in-vitro-function-of-satellite-cells-isolated-from-juvenile-porcine-muscles
#2
Claudia Miersch, Katja Stange, Monika Röntgen
Muscle stem cells, termed satellite cells (SC), and SC-derived myogenic progenitor cells (MPC) are involved in postnatal muscle growth, regeneration, and muscle adaptability. They can be released from their natural environment by mechanical disruption and tissue digestion. The literature contains several isolation protocols for porcine SC/MPC including various digestion procedures, but comparative studies are missing. In this report, classic trypsinization and a more complex trypsin, collagenase, and DNase (TCD) digestion were performed with skeletal muscle tissue from 4- to 5-d-old piglets...
May 21, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29766357/enhancement-of-calcification-by-osteoblasts-cultured-on-hydroxyapatite-surfaces-with-adsorbed-inorganic-polyphosphate
#3
Kan Kato, Koji Morita, Isao Hirata, Kazuya Doi, Takayasu Kubo, Koichi Kato, Kazuhiro Tsuga
Inorganic polyphosphate has been expected to accelerate bone regeneration. However, there are limited evidences to prove that polyphosphate adsorbed on the surface of a hydroxyapatite plate enhances calcification of cultured osteoblasts. In this study, we examined the effect of polyphosphate adsorbed onto the surface of a hydroxyapatite plate on the attachment, proliferation, differentiation, and calcification of osteoblasts. After hydroxyapatite plates were soaked in solutions of polyphosphate, the plate surfaces were analyzed by scanning electron microscopy and toluidine blue staining to confirm adsorption of polyphosphate...
May 15, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29748909/the-effect-of-storage-temperature-on-the-biological-activity-of-extracellular-vesicles-for-the-complement-system
#4
Sang June Park, Hyungtaek Jeon, Seung-Min Yoo, Myung-Shin Lee
Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions...
May 10, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29728912/effect-of-cryopreservation-on-the-appearance-and-liver-function-of-hepatocyte-like-cells-in-cultures-of-cirrhotic-liver-of-biliary-atresia
#5
Taisuke Yamazaki, Shin Enosawa, Takayoshi Tokiwa
Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of biliary atresia (BA) may contain stem/progenitor cells, and clusters of hepatocyte-like cells appear via hepatocyte growth factor/c-Met signaling in primary cultures of NPCs. BA is a rare and serious liver disease, and procurement of BA cells is difficult. Therefore, cryopreservation of BA liver cells is an unavoidable challenge. In this study, we examined the appearance and liver function of hepatocyte-like cells in cultures of BA liver-derived NPC fractions after cryopreservation for 1 or 6 mo using a chemically defined cryopreservation solution, STEM-CELLBANKER...
May 4, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29725883/production-of-progenitor-cells-from-primary-human-epithelial-cell-monolayer-cultures
#6
Atsuko Miyazawa, Shiuhyang Kuo, Stephen E Feinberg
Primary keratinocytes derived from human epidermis are widely used in tissue engineering and regenerative medicine. An important aspect in clinical applications is the preservation of human skin keratinocyte stem cells. However, it is difficult to expand the number of human skin keratinocyte stem cells, which are undifferentiated and highly proliferative in culture by using standard cell culture methods. It is even more difficult to identify them, since universal specific markers for human skin keratinocyte stem cells have not been identified...
May 3, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700744/animal-symposia-and-workshops
#7
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700743/plant-symposia-and-workshops
#8
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700742/plenary-symposia
#9
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700740/plant-contributed-papers
#10
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700739/keynote-symposium
#11
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700738/plant-posters
#12
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700737/education-silent-abstracts
#13
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700736/education-symposia-and-workshops
#14
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700735/animal-posters
#15
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29700734/animal-contributed-papers
#16
(no author information available yet)
No abstract text is available yet for this article.
April 26, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29654403/antitumor-activity-of-chitosan-from-mayfly-with-comparison-to-commercially-available-low-medium-and-high-molecular-weight-chitosans
#17
G Tan, M Kaya, A Tevlek, I Sargin, T Baran
Insects' cuticles have a potential to be evaluated as a chitin source. Especially adults of aquatic insects like mayflies (order Ephemeroptera) swarm in enormous numbers in artificially lit areas while mating in spring and then die by leaving huge amounts of dead insects' bodies. Here in this study, mayfly corpses were harvested and used for production of low MW chitosan. Dried mayfly bodies had 10.21% chitin content; mayfly chitin was converted into chitosan with efficiency rate of 78.43% (deacetylation degree, 84...
April 13, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29633080/culturing-conditions-highly-affect-dna-methylation-and-gene-expression-levels-in-mcf7-breast-cancer-cell-line
#18
Lama Hamadneh, May Al-Majawleh, Yazun Jarrar, Sawsan Shraim, Mariam Hasan, Basha'er Abu-Irmaileh
The levels of DNA methylation and their role in gene expression are key factors that could affect diagnosis, prognosis, and treatment options of different diseases. In this study, the methylation levels of 22 genes that are mostly correlated to breast cancer were determined using EpiTect methyl II PCR array. This analysis was performed to determine the effect of cells' passage number and the use of antibiotics in the culturing media on gene methylation levels in MCF7 cell line. DNA methylation levels of PTGS2, ADAM23, HIC1, and PYCARD were found to be significantly different among different passages...
April 9, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29626279/activation-of-adenosine-a2b-receptor-attenuates-high-glucose-induced-apoptosis-in-h9c2-cells-via-pi3k-akt-signaling
#19
Yi Shen, Gang Tang, Pan Gao, Bin Zhang, Hang Xiao, Liang-Yi Si
High glucose plays a vital role in apoptosis in H9C2 cells. However, the exact molecular mechanism remains unclear. In this study, we aimed to evaluate the cardio-protective role of A2b receptor in high glucose-induced cardiomyocyte apoptosis via PI3K/Akt pathway. Adenosine A2b receptor agonist (Bay506583), antagonist (MRS1754), and Akt inhibitor (LY294002) were applied respectively to H9C2 cells before exposed to high glucose for 12 h. Apoptosis of H9C2 cells was determined by TUNEL assay and the apoptosis rate by flow cytometry...
April 6, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29626278/establishment-and-proteomic-characterization-of-a-novel-synovial-sarcoma-cell-line-ncc-ss2-c1
#20
Rieko Oyama, Fusako Kito, Marimu Sakumoto, Kumiko Shiozawa, Shunichi Toki, Makoto Endo, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18-SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18-SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay...
April 6, 2018: In Vitro Cellular & Developmental Biology. Animal
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