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Colloids and Surfaces. B, Biointerfaces

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https://www.readbyqxmd.com/read/30419476/polydopamine-reinforced-hemostasis-of-a-graphene-oxide-sponge-via-enhanced-platelet-stimulation
#1
Guofeng Li, Yuping Liang, Congcong Xu, Hui Sun, Lei Tao, Yen Wei, Xing Wang
Graphene oxide (GO) is a promising hemostatic material because of its platelet stimulatory activity. However, our previous studies on cross-linked graphene sponges demonstrated that those sponges lost the GO function of platelet stimulation due to the pristine GO was reduced under the harsh reaction conditions. Accordingly, a mild cross-linking strategy is expected to preserve the oxygen-containing groups to further increase the hemostatic performance of the sponges. Here, we present a polydopamine (PDA) cross-linked GO sponge (DCGO) by using mild and facile wet chemistry...
November 2, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30412864/antibiofilm-efficacy-of-curcumin-in-combination-with-2-aminobenzimidazole-against-single-and-mixed-species-biofilms-of-candida-albicans-and-staphylococcus-aureus
#2
Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
Mixed fungal and bacterial biofilm associated infections of implants have been a huge challenge in health care because of the increased resistance to antimicrobials and the critical biological differences between fungi and bacteria. In this study, we evaluated the 2-aminobenzimidazole (2ABI) and curcumin (CUR) alone to inhibit planktonic cell growth, adhesion as well as single and mixed species biofilms of Candida albicans and Staphylococcus aureus on silicone. The combined effects between 2ABI and CUR on mixed species biofilm formation and pre-formed biofilm were assessed...
November 2, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30408675/the-effect-of-strontium-incorporation-into-sol-gel-biomaterials-on-their-protein-adsorption-and-cell-interactions
#3
F Romero-Gavilán, N Araújo-Gomes, I García-Arnáez, C Martínez-Ramos, F Elortza, M Azkargorta, I Iloro, M Gurruchaga, J Suay, I Goñi
It is known strontium can both inhibit the osteoclast formation and stimulate the osteoblast maturation, so biomaterials containing this element can favour bone structure stabilisation. The addition of Sr to biomaterials could affect their interactions with proteins and cells. Here, a silica-hybrid sol-gel network doped with different amounts of SrCl2 and applied as coatings on titanium discs was examined. in vitro analysis was performed to determine the potential effect of Sr in the coatings, showing enhanced gene expression of osteogenic markers (alkaline phosphatase and transforming growth factor-β) in MC3T3-E1 incubated with Sr-doped biomaterials...
November 1, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30399475/amphiphilic-quaternary-ammonium-chitosans-self-assemble-onto-bacterial-and-fungal-biofilms-and-kill-adherent-microorganisms
#4
Joonhoo Jung, Jianchun Wen, Yuyu Sun
Formation of biofilms on solid surfaces is a long-standing challenge to multiple medical and health-related applications. Once formed, biofilms are very difficult to destroy, and microorganisms in biofilms are much more resistant to antimicrobial agents than their free-floating counterparts. The current antimicrobial agents/disinfectants often have low residence time on biofilms, leading to low antimicrobial effect on biofilm microorganisms. We designed and synthesized an amphiphilic quaternary ammonium chitosan (CS612) as biocompatible antimicrobial agents that bind onto preformed biofilms to kill adherent microorganisms...
November 1, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30408674/reversing-multi-drug-tumor-resistance-to-paclitaxel-by-well-defined-ph-sensitive-amphiphilic-polypeptide-block-copolymers-via-induction-of-lysosomal-membrane-permeabilization
#5
Hassan Mostoufi, Gholamhossein Yousefi, Ali-Mohammad Tamaddon, Omidreza Firuzi
A series of hybrid di-block copolymers of poly(l- glutamic acid-b-l- leucine) (PGA-PLeu), methoxy poly (ethylene glycol)-b-poly(l-leucine) (PEG-PLeu), methoxy poly(ethylene glycol)-b-poly(γ-benzyl-l-glutamic acid) (PEG-PBLG) and tri-block copolymers of poly(ethylene glycol)-b-poly(l-glutamic acid-co-null- leucine) (PEG-PGA-PLeu) were synthesized through sequential HMDS-mediated ring-opening polymerization (ROP). Chemical structure of copolymers was studied by FTIR and 1 H-NMR and their molecular weight was determined by 1 H-NMR and gel permeation chromatography (GPC)...
October 26, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384272/synergistic-effects-of-metal-induced-aggregation-of-human-serum-albumin
#6
Yolanda S Hedberg, Illia Dobryden, Himanshu Chaudhary, Zheng Wei, Per M Claesson, Christofer Lendel
Exposure to cobalt (Co), chromium (Cr), and nickel (Ni) occurs often via skin contact and from different dental and orthopedic implants. The metal ions bind to proteins, which may induce structural changes and aggregation, with different medical consequences. We investigated human serum albumin (HSA) aggregation in the presence of CoII , CrIII , and/or NiII ions and/or their nanoparticle precipitates by using scattering, spectroscopic, and imaging techniques, at simulated physiological conditions (phosphate buffered saline - PBS, pH 7...
October 23, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384274/superactive-%C3%AE-galactosidase-inclusion-bodies
#7
Sandra S Flores, Verónica Nolan, María A Perillo, Julieta M Sánchez
Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific structures of unfolded proteins aggregates. Subsequent studies demonstrated that IBs contained an important quantity of active protein. In this work, we proved that recombinant β-galactosidase inclusion bodies (IBβ-Gal ) are functional aggregates. Moreover, they exhibit particular features distinct to the soluble version of the enzyme...
October 22, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384273/division-of-roles-of-modified-chains-in-organo-magnetic-nanoparticles-using-organo-modified-agents-having-hydrophilic-reactive-polar-groups-at-both-ends-formation-of-high-density-single-particle-layers-and-bioconjugation
#8
Takeru Yunoki, Yusuke Kimura, Atsuhiro Fujimori
The behavior of an organo-modified chain that changes its role depending on the environment has been investigated. Long-chain carboxylic acids having a hydrophilic reactive polar group at both ends were allowed to act on magnetic nanoparticles to produce organo-modified particles. These amphiphilic organo-magnetic nanoparticles are synthesized by the reaction of a hydroxyl terminal group on the outermost surface of the magnetic particle and a carboxylic acid. With spreading at the air/water interface, an extremely condensed single-particle layer on the water surface was formed...
October 22, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384265/fabrication-of-high-strength-mecobalamin-loaded-aligned-silk-fibroin-scaffolds-for-guiding-neuronal-orientation
#9
Luzhong Zhang, Li Xu, Guicai Li, Yumin Yang
Suitable micro/nanofibers orientation in tissue engineered scaffold could guide cells outgrowth and migration during tissue regeneration. However, it is still a challenge to prepare a simple, high-strength and aligned fibers scaffold. Herein, we report a facile strategy to construct high-strength silk fibroin (SF) scaffolds with aligned fibers orientation by crosslinking aligned SF fibers with regenerated SF solution for guiding neuronal growth direction. The fibers could be easily controlled within the same orientation...
October 22, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384264/antibacterial-ability-cytocompatibility-and-hemocompatibility-of-fluorinated-graphene
#10
Hao Geng, Tianbo Wang, Huiliang Cao, Hongqin Zhu, Zengfeng Di, Xuanyong Liu
Graphene halides are promising two-dimensional systems which have interesting physical and chemical properties. In particular, high quality fluorinated graphene offers a great potential in modulating variable properties by regulating its surface microstructure. Moreover, the fluorine introduction and carbon-fluorine bonding characters will enable some interesting biological response. Here, the biological responses to bacteria and cells of fluorinated graphene were studied. Present work revealed that partially fluorinated graphene behaved satisfactory antibacterial ability...
October 21, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384276/dual-triggered-drug-release-vehicles-for-synergistic-cancer-therapy
#11
Ting-Yu Tu, Shu-Jyuan Yang, Ming-Hsien Tsai, Chung-Hao Wang, Shin-Yu Lee, Tai-Horng Young, Ming-Jium Shieh
Cancer is a complex and tenacious disease. Drug-delivery systems in combination with multimodal therapy strategies are very promising candidates for cancer theranostic applications. In this study, a new drug-delivery vehicle that combine human serum albumin (HSA)- and poly(sodium 4-styrenesulfonate) (PSS)-coated gold nanorod nanoparticles(GNR/PSS/HSA NPs) was developed for synergistic cancer therapy. Doxorubicin (DOX) was loaded onto GNR/PSS/HSA NPs, by electrostatic and hydrophobic forces, to create multimodal DOX@GNR/PSS/HSA NPs...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384275/cefuroxime-conjugated-chitosan-hydrogel-for-treatment-of-wound-infections
#12
Vaishali Pawar, Mukesh Dhanka, Rohit Srivastava
Hydrogels are excellent drug delivery systems for the treatment of chronic wound infections. However, the problem of high burst release still remains a challenge that needs to be tackled. In terms of antibiotic release from the hydrogels, as the drug payload depletes it could act as a substrate for bacterial seeding which can create a life-threatening condition. Therefore, to provide the sustained effect of an antibiotic at the localized site via hydrogel matrix, we prepared a chitosan (CS) hydrogel system in which cefuroxime (CEF) is covalently conjugated with chitosan polymer via an ester linkage...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384268/structure-and-antibacterial-properties-of-ag-doped-micropattern-surfaces-produced-by-photolithography-method
#13
V A Ponomarev, N V Shvindina, E S Permyakova, P V Slukin, S G Ignatov, B Sirota, A A Voevodin, D V Shtansky
Photolithography methods offer ample opportunities for creating biological surface patterns over large areas. Herein, samples with patterned surface having the same Ag total coverage area and content, but different surface topography made of periodically spaced Ag/Si pillars with a diameter of 10 and 50 μm and a height of 3, 1, and 0.2 μm were produced by photolithography technique and studied to uncover the dependences of bactericide ion release on surface topography and antibacterial effect on Ag+ ion concentration...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384266/dendrimer-based-theranostic-nanostructures-for-combined-chemo-and-photothermal-therapy-of-liver-cancer-cells-in-vitro
#14
Artur Jędrzak, Bartosz F Grześkowiak, Emerson Coy, Jacek Wojnarowicz, Kosma Szutkowski, Stefan Jurga, Teofil Jesionowski, Radosław Mrówczyński
Here we report the synthesis of multifunctional nanocarriers based on PAMAM dendrimers generation (G) 4.0, 5.0 and 6.0 fixed to polydopamine (PDA) coated magnetite nanoparticles (Fe3 O4 ). Synthesized nanoplatforms were characterized by transmission electron microscopy (TEM), the electrokinetic (zeta) potential, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and magnetic resonance imaging (MRI). Further, we show as a proof of concept that nanocarriers functionalized with G 5...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384263/the-influence-of-the-essential-oil-extracted-from-hops-on-monolayers-and-bilayers-imitating-plant-pathogen-bacteria-membranes
#15
Karolina Połeć, Beata Barnaś, Magdalena Kowalska, Michał Dymek, Rafał Rachwalik, Elżbieta Sikora, Artur Biela, Michał Kobiałka, Kinga Wójcik, Katarzyna Hąc-Wydro
Many plant-derived compounds possess antimicrobial, antioxidant and even anticancer activities. Therefore, they are considered as substances that can be used instead of synthetic compounds in various applications. In this work, the essential oil from hop cones was extracted and analyzed, and then its effects on model bacteria membranes were studied to verify whether the hop essential oils could be used as ecological pesticides. The experiments involved surface pressure-area measurements, penetration studies and Brewster angle microscopy (BAM) imaging of lipid monolayers as well as hydrodynamic diameter, zeta potential, steady-state fluorescence anisotropy and Cryo-Transmission Electron Microscopy (cryo-TEM) measurements of liposomes...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30368212/green-solid-state-based-curcumin-mediated-rhamnolipids-stabilized-silver-nanoparticles-interaction-of-silver-nanoparticles-with-cystine-and-albumins-towards-fluorescence-sensing
#16
Dina Sadeq Al-Namil, Digambara Patra
Curcumin mediated rhamnolipids stabilized Ag Nanoparticles (NPs) have been successfully synthesized in a green solid-state procedure and in a solvent free environment. Depending on growth time, the synthesis process offers Ag NPs of different sizes and spherical shapes as reflected in the scanning electron microscope (SEM) images. The absorbance of rhamnolipids stabilized Ag NPs consistently increases with increase in growth time in rhamnolipids medium reconfirming growth in size of the particles. Even after 7 days of growth time in the rhamnolipids medium, the size slightly increases but the shapes remain spherical unlike the case observed in ethanolic environment...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30368211/investigation-on-adhesion-of-sulfobacillus-thermosulfidooxidans-via-atomic-force-microscopy-equipped-with-mineral-probes
#17
Qian Li, Thomas Becker, Ruiyong Zhang, Tangfu Xiao, Wolfgang Sand
Bacterial adhesion is a key step to prevent environmental problems called acid mine drainage or to improve leaching efficiency in industry, since it initiates and enhances bioleaching. Thus, to analyze bacterial adhesion and to understand this process is crucial. In this study atomic force microscopy equipped with a pyrite or chalcopyrite tip was applied to study the adhesion of Sulfobacillus thermosulfidooxidans. The results illustrate that planktonic cells of both pyrite- and sulfur-grown cells of S. thermosulfidooxidans show more affinity to pyrite than to chalcopyrite (adhesion forces 2 nN versus 0...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30368209/different-brij97-colloid-systems-as-potential-enhancers-of-acyclovir-skin-permeation-and-depot
#18
Elisabetta Mazzotta, Cesare Oliviero Rossi, Rita Muzzalupo
The low efficacy of Acyclovir topical therapy is due to its physicochemical properties that limit the permeation across the stratum corneum. The goal of this research was to evaluate the ability of biodegradable surfactant, Brij97, to self-assembly in different types of colloid systems which can improve the Acyclovir permeation and accumulation at the target site (the basal epidermis). New Acyclovir formulation based on Brij97 have been analyzed in order to investigate the effect of drug encapsulation on the structure...
October 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384269/box-behnken-design-of-sirna-loaded-liposomes-for-the-treatment-of-a-murine-model-of-ocular-keratitis-caused-by-acanthamoeba
#19
Giovanni K Zorzi, Roselena S Schuh, Vinicius J Maschio, Nathalya T Brazil, Marilise B Rott, Helder F Teixeira
Acanthamoeba keratitis is an ophthalmic disease with no specific treatment that specially affects contact lens users. The silencing of serine phosphatase (SP) and glycogen phosphorylase (GP) proteins produced by Acanthamoeba has been shown to significantly reduce the cytopathic effect, although no vehicle was proposed yet to deliver the siRNA sequences to the trophozoites. In this study, PEGylated cationic liposomes were proposed and optimized using Box-Behnken design. The influence of DOTAP:DOPE ratio, DSPE-PEG concentration, and siRNA/DOTAP charge ratio were evaluated over both biological response and physicochemical properties of liposomes...
October 18, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30384267/dual-effect-of-non-ionic-detergent-triton-x-100-on-insulin-amyloid-formation
#20
Katarina Siposova, Erik Sedlak, Tibor Kozar, Michal Nemergut, Andrey Musatov
Atomic force microscopy, Thioflavin T (ThT) fluorescence assay, circular dichroism spectroscopy, differential scanning calorimetry, and molecular modeling techniques have been employed to investigate the amyloid aggregation of insulin in the presence of non-ionic detergent, Triton X-100 (TX-100). In contrast to recently described inhibition of lysozyme amyloid formation by non-ionic detergents (Siposova, 2017), the amyloid aggregation of insulin in the presence of sub-micellar TX-100 concentration exhibits two dissimilar phases...
October 18, 2018: Colloids and Surfaces. B, Biointerfaces
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