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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/30597428/wavelet-analysis-of-higher-harmonics-in-tapping-mode-atomic-force-microscopy
#1
Zhenyu Wang, Jianqiang Qian, Yingzi Li, Yingxu Zhang, Zihang Song, Zhipeng Dou, Rui Lin
Higher harmonics have been widely used to characterize nanomechanical properties of the sample surface in tapping mode atomic force microscopy. They are usually analyzed by the Fourier transform method which provides time-averaged amplitude and phase information. In this paper, we apply the analytic wavelet transform to analyze higher harmonics. The intuitive descriptions of higher harmonics are obtained by the time-frequency analysis of the tip motion signal. The temporal evolutions of the higher harmonics are analyzed...
December 21, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30594435/preface-to-the-special-issue-on-liquid-phase-electron-microscopy
#2
EDITORIAL
Patricia Abellan, Niels de Jonge
No abstract text is available yet for this article.
December 21, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30590254/study-of-the-porosity-of-calcified-chicken-eggshell-using-atomic-force-microscopy-and-image-processing
#3
I Arzate-Vázquez, J V Méndez-Méndez, E A Flores-Johnson, J Nicolás-Bermúdez, J J Chanona-Pérez, E Santiago-Cortés
In this work, the porosity of the layers of calcified chicken eggshell (vertical crystal layer VCL, palisade layer PL and mammillary layer ML) was evaluated using atomic force microscopy (AFM) and image processing (IP). AFM topographic images were obtained from different locations for each layer and along the cross-section of calcified eggshell. Roughness parameters, surface area values, pore size and shape, surface porosity, area occupied by pores and pore density were obtained from AFM and IP. It was observed that the thickest layer (PL) exhibited the highest degree of porosity (surface porosity = 2...
December 21, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30572189/recent-advances-in-the-use-of-computed-tomography-in-concrete-technology-and-other-engineering-fields
#4
Miguel A Vicente, Dorys C González, Jesús Mínguez
Over the past two decades, immense research efforts at a global level have extended CT-Scan technology across several engineering fields. The state-of-the-art of the most relevant research related to the use of CT-Scanning is presented in this paper, which explores microstructural studies of materials used in various fields of engineering, with especial emphasis on concrete technology. Its main aim is to present the range of new applications, in addition to the conventional uses of CT-Scan technology. Based on X-ray absorption, CT generates a visual display of the internal microstructure of a material at micro-range resolutions...
December 12, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30583220/examination-of-focused-ion-beam-induced-damage-during-platinum-deposition-in-the-near-surface-region-of-an-aerospace-aluminum-alloy
#5
Adam W Thompson, Zachary D Harris, James T Burns
It is well known that damage induced by impinging Ga+ ions during focused ion beam (FIB) milling of transmission electron microscopy (TEM) specimens can obfuscate subsequent TEM characterization, especially in the near-surface region of the TEM foil. Numerous strategies for minimizing this damage have been invoked, with the most common being the deposition of a Pt 'strap' at the area of interest. However, damage can still occur in the near-surface region during this Pt deposition step and the variation in the character and extent of this damage with applied Pt deposition parameter, especially in complex structural alloys, is not well characterized...
December 11, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30550820/best-practices-for-preparing-radioactive-specimens-for-ebsd-analysis
#6
Tanvi Ajantiwalay, Tammy Trowbridge, Alexander Winston, Cheng Sun, Kumar Sridharan, Assel Aitkaliyeva
A wide variety of specimen preparation techniques are available for ensuring that specimen surface finish has the acceptable quality for electron backscatter diffraction (EBSD) analysis. These techniques include but are not limited to vibratory polishing, broad, and focused ion beam milling. They have been widely implemented in the field of nuclear materials science with a varying degree of success. However, a systematic investigation of the effectiveness of each technique for preparation of highly radioactive specimens has not been conducted to date but would be beneficial during selection of the specimen preparation methodology...
December 7, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30572190/addressing-some-of-the-technical-challenges-associated-with-liquid-phase-s-tem-studies-of-particle-nucleation-growth-and-assembly
#7
Guomin Zhu, Holger Reiner, Helmut Cölfen, James J De Yoreo
In situ liquid phase scanning/ transmission electron microscopy (LP-S/TEM), enables direct observation of particle formation and evolution in the hydrated liquid state. Though powerful, this technique has significant technical limitations, which need to be carefully addressed in order to obtain reliable quantitative data. In this paper, we highlight several common challenges seen in LP-TEM, including electron-beam induced particle dissolution and motion, particle-membrane adhesion, contamination, and triggering of reactions...
December 6, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30502653/electron-tomography-on-latex-particles-suspended-in-water-using-environmental-scanning-electron-microscopy
#8
X Jiao, L Roiban, G Foray, K Masenelli-Varlot
Tilted image series are acquired on liquid suspensions of latex particles supported by a carbon membrane. To do this, a home-made stage fitting in an Environmental Scanning Electron Microscope (ESEM) is optimized to minimize cold losses and thus improve both sample stability and spatial resolution in the images. As latex particles are electron-sensitive materials, sample degradation and stability are taken into account in the choice of the parameters for tilted image series acquisition. Despite the limited number of images, conventional algorithms are used to reconstruct the volume and study the three-dimensional arrangement of latex particles...
November 24, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30553186/primary-cultured-neuronal-networks-and-type-2-diabetes-model-mouse-fatty-liver-tissues-in-aqueous-liquid-observed-by-atmospheric-sem-asem-staining-preferences-of-metal-solutions
#9
Chikara Sato, Toshiko Yamazawa, Akiko Ohtani, Yuusuke Maruyama, Nassirhadjy Memtily, Mari Sato, Yuri Hatano, Takashi Shiga, Tatsuhiko Ebihara
Neural networking, including axon targeting and synapse formation, is the basis of various brain functions, including memory and learning. Diabetes-mellitus affects peripheral nerves and is known to cause fatty liver disease. Electron microscopy (EM) provides the resolution required to observe changes in fine subcellular structures caused by such physiological and pathological processes, but samples are observed in vacuum. Environmental capsule EM can directly observe cells in a more natural aqueous environment, but the size-limited capsules restrict cell culturability...
November 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30504080/dynamics-of-gold-nanoparticle-clusters-observed-with-liquid-phase-electron-microscopy
#10
Elisa Cepeda-Pérez, Niels de Jonge
The dynamics of processes of nanoparticles such as diffusion, attraction and repulsion, and self-assembly of structures of nanoparticles at the solid-liquid interfaces differ significantly from those occurring for bulk conditions and their fundamental physical rules are still unknown. Here, we used liquid phase scanning transmission electron microscopy (LP-STEM) to study several aspects of nanoparticle dynamics of colloidal chitosan coated gold nanoparticle (TCHIT-AuNP) clusters in a liquid layer enclosed between two SiN membranes...
November 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30472499/human-skeletal-muscle-cell-atlas-unraveling-cellular-secrets-utilizing-muscle-on-a-chip-differential-expansion-microscopy-mass-spectrometry-nanothermometry-and-machine-learning
#11
Bhanu P Jena, Domenico L Gatti, Suzan Arslanturk, Sebastian Pernal, Douglas J Taatjes
The 'Human Cell Atlas' project has been launched to obtain a comprehensive understanding of all cell types, the fundamental living units that constitute the human body. This is a global partnership and effort involving experts from many disciplines, from computer science, engineering to medicine, and is supported by several private and public organizations, among them, the Chan Zuckerberg Foundation, the National Institutes of Health, and Google, that will greatly benefit humanity. Nearly 37 trillion cells of various shapes, sizes, and composition, are precisely organized to constitute the human body...
November 16, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30472498/single-shot-real-time-sub-nanosecond-electron-imaging-aided-by-compressed-sensing-analytical-modeling-and-simulation
#12
Xianglei Liu, Shian Zhang, Aycan Yurtsever, Jinyang Liang
Bringing ultrafast (nanosecond and below) temporal resolution to transmission electron microscopy (TEM) has historically been challenging. Despite significant recent progress in this direction, it remains difficult to achieve sub-nanosecond temporal resolution with a single electron pulse, in real-time (i.e., duration in which the event occurs) imaging. To address this limitation, here, we propose a methodology that combines laser-assisted TEM with computational imaging methodologies based on compressed sensing (CS)...
November 16, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30468967/attachment-of-iron-oxide-nanoparticles-to-carbon-nanofibers-studied-by-in-situ-liquid-phase-transmission-electron-microscopy
#13
Nynke A Krans, N Ahmad, D Alloyeau, K P de Jong, J Zečević
By using liquid phase transmission electron microscopy (LP-TEM), the dynamics of iron oxide nanoparticle (Fe-NP) attachment to carbon nanofibers (CNFs) and oxygen functionalized CNFs (CNF-Ox) were studied in-situ. The beam effect on the stability of the sample in various liquids was examined, and it was found that toluene provided the highest stability and resolution to image both CNF supports and Fe-NPs. Flowing particles dispersed in toluene through the liquid cell allowed direct monitoring of the attachment process at ambient temperature...
November 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30458300/palaeopermeability-anisotropy-and-geometrical-properties-of-sealed-microfractures-from-micro-ct-analyses-an-open-source-implementation
#14
Rodrigo Gomila, Gloria Arancibia, Domingo Mery, Mathias Nehler, Rolf Bracke, Diego Morata
Fault zone permeability and the real 3D-spatial distribution of the fault-related fracture networks are critical in the assessment of fault zones behavior for fluids. The study of the real 3D-spatial distribution of the microfracture network, using X-ray micro-computed tomography, is a crucial factor to unravel the real structural permeability conditions of a fault-zone. Despite the availability of several commercial software for rock properties estimation from X-ray micro-computed tomography scanning, their high cost and lack of programmability encourage the use of open-source data treatment...
November 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30408701/temuc3-a-computer-program-for-unit-cell-determination-of-crystalline-phases-in-tem-experiments
#15
X-Z Li
A new computer program, TEMUC3, has been developed for unit-cell determination of crystal phases using electron diffraction data. A graphic user-friendly interface was designed for the following operations, (i) a reciprocal lattice is reconstructed from a tilt series of electron diffraction patterns; (ii) a Niggli cell is generated from the two electron diffraction patterns in a tilt series; iii) a Niggli cell is generated from three electron diffraction patterns, in which each pair shares a common reflection vector; (iv) a conventional unit-cell is converted from a Niggli cell; (v) a unit-cell is refined from a set of reflections...
November 2, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30419433/considerations-for-imaging-thick-low-contrast-and-beam-sensitive-samples-with-liquid-cell-transmission-electron-microscopy
#16
Trevor H Moser, Tolou Shokuhfar, James E Evans
Transmission electron microscopy of whole cells is hindered by the inherently large thickness and low atomic contrast intrinsic of cellular material. Liquid cell transmission electron microscopy allows samples to remain in their native hydrated state and may permit visualizing cellular dynamics in-situ. However, imaging biological cells with this approach remains challenging and identifying an optimal imaging regime using empirical data would help foster new advancements in the field. Recent questions about the role of the electron beam inducing morphological changes or damaging cellular structure and function necessitates further investigation of electron beam-cell interactions, but such comparisons are complicated by variability in imaging techniques used across various studies currently present in literature...
November 1, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30419432/formation-of-gold-nanoparticles-in-a-free-standing-ionic-liquid-triggered-by-heat-and-electron-irradiation
#17
Debora Keller, Trond R Henninen, Rolf Erni
Ionic liquids (ILs) feature negligibly low vapor pressures and can thus be freely introduced into the high vacuum of a transmission electron microscope. With this extraordinary property, the ILs offer a powerful tool for in situ transmission electron microscopy (TEM) in window-free liquid media at very high resolution. In this work, we use the IL 1-butyl-3-methylimidazolium chloride in order to study nucleation and growth of gold nanoparticles (NPs) in free-standing liquid droplets by scanning TEM (STEM). The results confirm that the used IL allows for generating Au NPs in situ, triggered by electron irradiation and heat...
November 1, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30447491/tomographic-analysis-of-siliceous-particulates-in-australian-turpentine-wood-syncarpia-glomulifera-through-x-ray-micro-computed-tomography
#18
Tim Koddenberg, Kim Christian Krause, Andreas Krause
X-ray micro-computed tomography (XμCT) allows a non-destructive and three-dimensional (3D) study of otherwise complex and opaque wood tissues. In wood research, XμCT datasets are highly useful for the qualitative and quantitative examination of wood structures. In this study, XμCT was introduced and tested for examining X-ray dense silica particles in the Australian turpentine wood (Syncarpia glomulifera). It was possible to three-dimensionally visualize and numerically quantify silica particles. Numerical analysis was performed to scrutinize the size and content of silica particles...
October 28, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30253318/micro-and-nanoscale-characterization-of-poly-dl-lactic-co-glycolic-acid-films-subjected-to-the-l929-cells-and-the-cyclic-mechanical-load
#19
Marcin K Heljak, Maryla Moczulska-Heljak, Emilia Choińska, Adrian Chlanda, Alicja Kosik-Kozioł, Tomasz Jaroszewicz, Jakub Jaroszewicz, Wojciech Swieszkowski
In this paper, the effect of the presence of L929 fibroblast cells and a cyclic load application on the kinetics of the degradation of amorphous PLGA films was examined. Complex micro and nano morphological, mechanical and physico-chemical studies were performed to assess the degradation of the tested material. For this purpose, molecular weight, glass transition temperature, specimen morphology (SEM, μCT) and topography (AFM) as well as the stiffness of the material were measured. The study showed that the presence of living cells along with a mechanical load accelerates the PLGA degradation in comparison to the degradation occurring in acellular media: PBS and DMEM...
December 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30142494/micro-and-nanostructural-analysis-of-a-human-tooth-using-correlated-focused-ion-beam-fib-and-transmission-electron-microscopy-tem-investigations
#20
Meltem Sezen, Cleva Ow-Yang, Özlem Karahan, Burak Kıtıki
In this study, natural molar human tooth specimens were investigated for determining their micro- and nanoscale structural morphology, chemistry and crystallinity. The differences were tracked comparatively for both enamel and dentin layers and at their interfaces. Although dental material structures are hard and tough and the cross-sectioning of these materials using mechanical methods is challenging, FIB-SEM dual-beam instruments serve for preparing ultra-thin homogenous lamella sections. In this work, both FIB-SEM and TEM based advanced characterization methods were applied to reveal different morphological characteristics of dental tissue via complementary imaging and diffraction analysis...
December 2018: Micron: the International Research and Review Journal for Microscopy
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