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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/30223177/phase-transition-and-hardness-evolution-of-a-ti-5al-5mo-1fe-1cr-alloy-subjected-to-isothermal-aging
#1
Zongyu Xie, Xiaoqin Ou, Song Ni, Min Song
In this paper, a Ti-5Al-5Mo-1Fe-1Cr alloy was isothermally aged at 500 °C for different periods. Phase transformation took place during the aging treatment. The phase transformation was studied by X-ray diffraction spectroscopy and transmission electron microscopy. The as-quenched sample was composed of matrix β (body-centered cubic structure) phase, ωath (hexagonal structure) phase and minor α (hexagonal close-packed structure) phase. The results showed that aging treatment promoted the β → ω → α phase transformations in the as-quenched alloys...
September 11, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30219738/preparation-of-surfaces-of-composite-samples-for-tip-based-micro-analyses-using-ion-beam-milling
#2
Jan Pinc, Marcela Dendisová, Kateřina Kolářová, Ondrej Gedeon, Marie Švecová, David Hradil, Janka Hradilová, Vilém Bartůněk
Ion beam milling, as a method of surface design for tip analytical techniques, was explored. A sample of clay, embedded in a resin, was treated by the ion beam and allowed AFM (a typical tip technique) to be successfully applied. The method is suitable for advanced tip analyses based on AFM, like TERS or SNOM, and for samples that are not possible to prepare by standard mechanical methods. The approach can be useful for characterisation of the surfaces of many different types of materials in versatile applications such as catalysis, corrosion science or advanced material characterisation...
September 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30219739/fast-finecut-grain-boundary-detection-in-microscopic-images-considering-3d-information
#3
Boyuan Ma, Xiaojuan Ban, Ya Su, Chuni Liu, Hao Wang, Weihua Xue, Yonghong Zhi, Di Wu
The inner structure of a material is called its microstructure. It stores the genesis of a material and determines all the physical and chemical properties. However, the microstructure is highly complex and numerous image defects such as vague or missing boundaries formed during sample preparation, which makes it difficult to extract the grain boundaries precisely. In this work, we address the task of grain boundary detection in microscopic image processing and develop a graph-cut based method called Fast-FineCut to solve the problem...
September 7, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30199746/morphology-and-ultrastructure-of-the-infrabuccal-pocket-and-its-lining-epithelium-in-workers-of-ectomomyrmex-javanus-hymenoptera-formicidae
#4
Chu Wang, Johan Billen, Xingran Pan, Hong He
This paper describes the morphology and ultrastructure of the infrabuccal pocket in workers of the carnivorous ant Ectomomyrmex javanus, using SEM and TEM. The infrabuccal pocket is a flexible bag with a diameter of 330 ± 30 μm. In its anterior part, the pocket wall reaches a thickness of 24.45 ± 3.45 μm, which is thicker than the wall lining the rest of the infrabuccal pocket, where it measures 6.87 ± 1.12 μm. The epidermal cells of the wall form a thickened epithelium with a thickness of 10...
September 5, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30212712/in-situ-oxidation-and-reduction-of-cerium-dioxide-nanoparticles-studied-by-scanning-transmission-electron-microscopy
#5
Aaron C Johnston-Peck, Wei-Chang D Yang, Jonathan P Winterstein, Renu Sharma, Andrew A Herzing
Cerium dioxide nanocubes and truncated octahedra were reduced and oxidized in the scanning transmission electron microscope. The reduction process was stimulated by the electron beam and oxidation was supported by background gases in the microscope environment. High-angle annular dark field imaging is sensitive to local lattice distortions that arise as oxygen vacancies are created and cerium cations reduce enabling high spatial resolution characterization of this process with temporal resolution on the order of seconds...
August 30, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30173001/a-consistent-path-for-phase-determination-based-on-transmission-electron-microscopy-techniques-and-supporting-simulations
#6
Lukas Konrad, Haishuang Zhao, Christian Gspan, John Rehr, Ute Kolb, Martina Lattemann, Gerald Kothleitner
This work addresses aspects for the analysis of industrial relevant materials via transmission electron microscopy (TEM). The complex phase chemistry and structural diversity of these materials require several characterization techniques to be employed simultaneously; unfortunately, different characterization techniques often lack connection to yield a complete and consistent picture. This paper describes a continuous path, starting with the acquisition of 3D diffraction data - alongside classical high-resolution imaging techniques - and linking the structural characterization of hard metal industrial samples with energy-loss fine-structure simulations, quantitative electron energy-loss (EEL) and energy-dispersive X-ray (EDX) spectroscopy...
August 24, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30153644/fine-structure-of-the-gametes-and-spermiogenesis-in-spiophanes-uschakowi-annelida-spionidae-from-the-sea-of-japan-with-comments-on-fertilization-biology-in-broadcast-spawning-spionids
#7
Vasily I Radashevsky, Olga V Yurchenko, Yana N Alexandrova
Spiophanes uschakowi is a common polychaete living in tubes in sandy sediments in shallow waters of the Sea of Japan. Females and males release their gametes into the water where fertilization and holopelagic, planktotrophic larval development occur. In females, oogenesis is intraovarian: vitellogenesis occurs when the oocytes grow in paired ovaries attached to genital blood vessels in fertile segments. The developed oocytes are accumulated in the coelomic cavity prior to spawning. The newly released oocytes are lentiform, 185-200 μm in diameter, with honey-combed envelopes 5-7 μm thick...
August 18, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30149294/nanoscale-investigation-by-tem-and-stem-eels-of-the-laser-induced-yellowing
#8
Marie Godet, Véronique Vergès-Belmin, Nicolas Gauquelin, Mandana Saheb, Judith Monnier, Eric Leroy, Julie Bourgon, Johan Verbeeck, Christine Andraud
Nd-YAG QS laser cleaning of soiled stone at 1064 nm can sometimes result in a more yellow appearance compared to other cleaning techniques. Especially in France, this yellowing effect is still considered as a major aesthetic issue by the architects and conservators. One explanation states that the yellowing is linked to the formation of iron-rich nanophase(s) through the laser beam interaction with black crusts that would re-deposit on the cleaned substrate after irradiation. To characterize these nanophases, a model crust containing hematite was elaborated and laser irradiated using a Nd-YAG QS laser...
August 18, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30142494/micro-and-nanostructural-analysis-of-a-human-tooth-using-correlated-focused-ion-beam-fib-and-transmission-electron-microscopy-tem-investigations
#9
Meltem Sezen, Cleva Ow-Yang, Özlem Karahan, Burak Kıtıki
In this study, natural molar human tooth specimens were investigated for determining their micro- and nanoscale structural morphology, chemistry and crystallinity. The differences were tracked comparatively for both enamel and dentin layers and at their interfaces. Although dental material structures are hard and tough and the cross-sectioning of these materials using mechanical methods is challenging, FIB-SEM dual-beam instruments serve for preparing ultra-thin homogenous lamella sections. In this work, both FIB-SEM and TEM based advanced characterization methods were applied to reveal different morphological characteristics of dental tissue via complementary imaging and diffraction analysis...
August 16, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30138769/does-magnification-of-sem-image-influence-quantification-of-particulate-matters-deposited-on-vegetation-foliage
#10
Lin Lin, Jingli Yan, Guojian Chen, Rongli Tang, Le Bao, Keming Ma, Weiqi Zhou, Xiu Yuan, Zhe Yin, Shuwen Zhou
The metrics used to quantify the particles on the leaf surface can serve as indicators for the quality of the atmospheric environment and is employed to evaluate the ecosystem services that plants can provide. Magnification of an SEM image may affect the recognition of particles in various aspects, yet little research has examined the impact. In this study, images were obtained at magnifications of 500×, 1000×, 2000×, and 5000× for a widely planted vegetation fence species in Beijing: Euonymus japonicas...
August 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30130739/transmission-electron-microscopy-investigations-of-the-microstructures-in-rapidly-solidified-mg-sn-ribbons
#11
Li Ye, Yuanlin Zhuang, Dongshan Zhao, Shuangfeng Jia, Jiaping Zhou, Jianian Gui, Jianbo Wang
Ribbons of an Mg-9.76 wt.%Sn alloy have been fabricated using rapid solidification technology. X-ray diffraction and transmission electron microscopy confirm that the rapidly solidified ribbons consist of α-Mg, β-Mg2 Sn and β″ -Mg3 Sn phases. The α-Mg grains are refined in the ribbons. The predominant fraction of the β-Mg2 Sn phase distributes at the grain boundaries of the α-Mg grains. The minor fraction of the β-Mg2 Sn phase reveals a spherical morphology with a typical grain size of 170 nm. The orientation relationship between the β″ -Mg3 Sn particles and α -Mg matrix is identified in the ribbons and an atomic structure model of the β″ -Mg3 Sn phase is proposed...
August 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30096632/a-study-about-color-normalization-methods-for-histopathology-images
#12
REVIEW
Santanu Roy, Alok Kumar Jain, Shyam Lal, Jyoti Kini
Histopathology images are used for the diagnosis of the cancerous disease by the examination of tissue with the help of Whole Slide Imaging (WSI) scanner. A decision support system works well by the analysis of the histopathology images but a lot of problems arise in its decision. Color variation in the histopathology images is occurring due to use of the different scanner, use of various equipments, different stain coloring and reactivity from a different manufacturer. In this paper, detailed study and performance evaluation of color normalization methods on histopathology image datasets are presented...
August 1, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30103076/a-three-dimensional-geometric-quantification-of-human-cortical-canals-using-an-innovative-method-with-micro-computed-tomographic-data
#13
X Roothaer, R Delille, H Morvan, B Bennani, E Markiewicz, C Fontaine
The complex architecture of bone has been investigated for several decades. Some pioneer works proved an existing link between microstructure and external mechanical loading applied on bone. Due to sinuous network of canals and limitations of experimental acquisition technique, there has been little quantitative analysis of three-dimensional description of cortical network. The aim of this study is to provide an algorithmic process, using Python 3.5, in order to identify 3D geometrical characteristics of voids considered as canals...
July 25, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30056255/multi-scale-characterization-and-biological-evaluation-of-composite-surface-layers-produced-under-glow-discharge-conditions-on-niti-shape-memory-alloy-for-potential-cardiological-application
#14
Adrian Chlanda, Justyna Witkowska, Jerzy Morgiel, Katarzyna Nowińska, Emilia Choińska, Wojciech Swieszkowski, Tadeusz Wierzchoń
NiTi shape memory alloys are characterized by relatively good biocompatibility primarily thanks to their ability to self-passivate. However, before they can be used as medical implants for long term use, they need to undergo treatment aimed at producing layers on their surface that are superior to spontaneously formed oxide layers and that would increase their resistance to corrosion, limit nickel ion release from the surface (metallosis) and have the capability to shape their biological properties depending on the application...
July 24, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30075415/atomic-scale-3d-reconstruction-of-antiphase-boundaries-in-gap-on-001-silicon-by-stem
#15
Jürgen Belz, Andreas Beyer, Kerstin Volz
In order to overcome the limitations of silicon-based electronics, the integration of optically active III-V compounds is a promising approach. Nonetheless, their integration is far from trivial and control as well as understanding of corresponding growth kinetics, and in particular the occurrence and termination of antiphase defects, is of great relevance. In this work, we focus on the three-dimensional reconstruction of such boundaries in gallium phosphide from single scanning transmission electron microscopy images...
July 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30053717/a-fast-imaging-method-of-scanning-ion-conductance-microscopy
#16
Gongxin Li, Xing Fang
Scanning ion conductance microscopy (SICM) has attracted considerable attention in the biological field as a noninvasive, high-resolution and non-force contact imaging technology. However, the development of improvement to the SICM imaging rate remains a great challenge for applications of rapid or dynamic imaging. In this paper, a fast SICM imaging method is proposed to improve the imaging efficiency via the design of a compressive sampling strategy and a reduction in the reconstruction time of sparse signals using the 2D normalized iterative hard thresholding (2D-NIHT) algorithm...
July 20, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30028972/nets-detection-and-quantification-in-paraffin-embedded-samples-using-confocal-microscopy
#17
A Santos, P Martín, A Blasco, J Solano, B Cózar, D García, J Goicolea, C Bellas, M J Coronado
Detection and quantification of Neutrophil Extracellular Traps (NETs) in tissue samples has become a topic of great interest to understand their pathological role in various diseases. We describe a semi-automatic method of visualization and quantification of NETs in paraffin-embedded intracoronary thrombus aspirate samples. This study is based on colocalization of myeloperoxidase (MPO) and citrullinated histone 3 (H3Cit) as hallmark of the presence of NETs. For the analysis we used the confocal immunofluorescence microscopy technology to quantify the number of fields and the total area (in μm2 ) containing NETs in each thrombus sample...
July 17, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30059830/exit-wave-phase-retrieval-from-a-single-high-resolution-transmission-electron-microscopy-image-of-a-weak-phase-object
#18
F Lin, X B Ren, W P Zhou, L Y Zhang, Y Xiao, Q Zhang, H T Xu, H Li, C H Jin
We propose a novel algorithm to numerically retrieve the phase of the exit-wave function from a high-resolution transmission electron microscopy (HRTEM) image of a weak-phase object material, e.g., graphene and hexagonal boron nitride monolayers. It theoretically only requires a single HRTEM image to retrieve the phase under the assumption of a weak-phase object. In addition, it can remove the effects of geometrical aberrations up to fifth order, and also improve the degraded information due to the finite temporal and spatial coherence...
July 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30119824/erratum-to-basic-properties-of-atp-induced-myosin-head-movement-in-hydrated-myosin-filaments-studied-using-the-gas-environmental-chamber-micron-113-2018-48-60
#19
H Sugi, T Akimoto, S Chaen
No abstract text is available yet for this article.
October 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30015016/multiple-ellipse-fitting-method-to-precisely-measure-the-positions-of-atomic-columns-in-a-transmission-electron-microscope-image
#20
Q Zhang, C H Jin, H T Xu, L Y Zhang, X B Ren, Y Ouyang, X J Wang, X J Yue, F Lin
In this paper, we propose a multiple-ellipse fitting method to accurately determine the atomic column positions in transmission electron microscopy (TEM) images. The column is enclosed by a series of ellipses fitted from contour lines at equidistant intensity levels, and each atomic column is shaped by an averaged elliptical shape to obtain its positions. In particular, the intensity profile of the atomic column can be obtained by an elliptically rotational average based on its shape; therefore, the intensities of the neighbouring atomic column can be subtracted for each atomic column during subsequent position refinement...
October 2018: Micron: the International Research and Review Journal for Microscopy
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