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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/30408701/temuc3-a-computer-program-for-unit-cell-determination-of-crystalline-phases-in-tem-experiments
#1
X-Z Li
A new computer program, TEMUC3, has been developed for unit-cell determination of crystal phases using electron diffraction data. A graphic user-friendly interface was designed for the following operations, (i) a reciprocal lattice is reconstructed from a tilt series of electron diffraction patterns; (ii) a Niggli cell is generated from the two electron diffraction patterns in a tilt series; iii) a Niggli cell is generated from three electron diffraction patterns, in which each pair shares a common reflection vector; (iv) a conventional unit-cell is converted from a Niggli cell; (v) a unit-cell is refined from a set of reflections...
November 2, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30352362/mapping-the-brazilian-microscopy-landscape-a-bibliometric-and-network-analysis
#2
Priscila C Albuquerque, Bruna de Paula Fonseca E Fonseca, Wendell Girard-Dias, Fabio Zicker, Wanderley de Souza, Kildare Miranda
Understanding how a technology is introduced and shared in a society has a strategic value for the planning of technological development and assessing new market opportunities. Among other technologies, microscopy has had a significant role in advancing different fields of science. In Brazil, its use spans from biomedical to engineering areas. Here, we used social network analysis (SNA) to map and quantify the flow of interaction between Brazilian researchers involved in microscopy techniques. The analysis examines co-occurrence of thematic networks and scientific co-authorship in articles published in a ten years window, as retrieved from Scopus database...
October 15, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30366196/finite-element-modeling-of-living-cells-for-afm-indentation-based-biomechanical-characterization
#3
Yi Liu, Keyvan Mollaeian, Juan Ren
Mechanotransduction-the process living cells sense and respond to forces-is essential for maintenance of normal cell, tissue, and organ functioning. To promote the knowledge of mechanotransduction, atomic force microscope (AFM) force-indentation has been broadly used to quantify the mechanical properties of living cells. However, most studies treated the cells as a homogeneous elastic or viscoelastic material, which is far from the real structure of cells, and the quantified mechanical properties cannot be used to investigate the inner working mechanism of mechanotransduction, such as internal force distribution/transduction...
October 14, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30368200/effect-of-electron-beam-irradiation-in-tem-on-the-microstructure-and-composition-of-nanoprecipitates-in-al-mg-si-alloys
#4
Haonan Chen, Kai Li, Mingjun Yang, Zhuo Zhang, Yi Kong, Qiang Lu, Yong Du
The evolution of nanoprecipitates β″ to disordered precipitates under electron beam irradiation in transmission electron microscope (TEM) has been studied. High-resolution TEM images show that β″ precipitates became disordered and diminished gradually under the 200 keV or 300 keV electron beam but displayed no significant changes under 80 keV. Under the same energy, the electron beam irradiation damage to the β″ was found as a function of electron dose. Knock-on damage and diffusion rather than radiolysis or heating effect are the main reasons for the disordering of β″...
October 9, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30366195/surface-potential-and-charging-of-polymer-films-submitted-to-defocused-electron-beam-irradiation
#5
Jing Liu, Hai-Bo Zhang, Yue-Hu Ding, Zhong Yan, Ji-Sheng Tong, Ye Yuan, Qing Zhao
The surface potentials and dynamic charging properties of polymers subjected to electron irradiation in scanning electron microscopy are investigated by using a novel method applied in parallel with simulation and experimental methods. We perform our simulation through a parallel computing method with various microscopic parameters. The results show that under the given microscopic parameters, the charging time concomitantly decreases as the film thickness, electron mobility, trap cross and recombination rate increase...
October 9, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30366197/two-dimensional-mapping-of-the-electric-field-distribution-inside-vacuum-microgaps-observed-in-a-scanning-electron-microscope
#6
Guodong Meng, Chengye Dong, Xinyu Gao, Dujiao Zhang, Kejing Wang, Pengcheng Zhang, Yonghong Cheng
In this paper, we present an in-situ measurement method to directly observe the distribution of the local electric field between vacuum microgaps. The measurement was performed in-situ inside a high resolution scanning electron microscope (SEM), and the nature of the local electric field was characterized through secondary electron contrast images with the aid of Rutherford scattering theory. Based on the regular fringes in these contrast images, the distribution of the local electric field could be extracted from the contour lines of the fringes while the magnitude of the local electric field could be evaluated qualitatively by the gradient of the contour lines...
October 3, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30308332/effects-of-carvacrol-on-oocyte-development-in-semi-engorged-rhipicephalus-sanguineus-sensu-lato-females-ticks-acari-ixodidae
#7
José Ribamar Lima de Souza, Patrícia Rosa de Oliveira, Luís Adriano Anholeto, André Arnosti, Erik Daemon, Rafael Neodini Remedio, Maria Izabel Camargo-Mathias
Currently, the most commonly used method to control ticks is the use of synthetic acaricides. However, these compounds are potentially harmful to hosts and the environment, in addition to causing the selection of resistant individuals. Therefore, several studies have been conducted to find sustainable methods to control ticks, such as Rhipicephalus sanguineus sensu lato, the most important vectors of pathogens for dogs. There has been increasing research on natural products with acaricidal action, especially with regard to plant-derived compounds as carvacrol, an aromatic monoterpene with several biological properties, including repellent and acaricidal activities, besides exerting cytotoxic effects on the exposed ticks...
September 29, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30300825/ion-transmission-through-a-dielectric-hollow-tip-for-scanning-probe-microscopy
#8
B N Mironov, S A Aseyev, S V Chekalin
We present a new concept for scanning probe microscopy characterization of molecular microstructures. It is based on a thin capillary using as a sharp tip to probe and map the morphology of a surface. In our experiment a collimated ion beam is formed by tungsten ions passing through a quartz tapered capillary with a 100-nm aperture and enters a 2D position-sensitive detector. We demonstrate that such ions are capable of producing the image of a dielectric nanoaperture in the case of low-dose ion beam. Ion transmission through a nanoscale capillary opens the door to observing photodesorption of large organic molecular ions with high spatially-element resolution using the combination of a hollow-tip vacuum scanner with time-of-flight mass spectrometer...
September 29, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30321742/electron-microscopy-determination-of-crystallographic-polarity-of-aluminum-nitride-thin-films
#9
Noriyuki Kuwano, Jesbains Kaur, Siti Rahmah
Aluminum nitride (AlN) crystallizes usually in the wurtzite structure (P63 mc) and it has a crystallographic polarity. In this work, the polarity in AlN was characterized by using several methods of transmission electron microscopy (TEM) in order to examine their applicability. AlN was deposited by metalorganic vapor phase epitaxy (MOVPE), followed by annealing at 1550 °C. TEM samples were prepared by using a focused ion beam (FIB) mill. Observation was performed with microscopes of JEM-2100, JEM-ARM200 F and FEI Titan Cubed G2 at Kyushu University (Japan), and the following results were obtained...
September 28, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30296728/space-quantization-between-the-object-and-image-spaces-of-a-microscopic-stereovision-system-with-a-stereo-light-microscope
#10
Yuezong Wang, Zhizhong Zhao
Microscopic stereovision systems with a stereo light microscope have been used for quantized observations and measurements in microscopy fields. These applications depend on a quantization mathematical relationship between the object space and the image space. In this article, a novel, practical space quantization method was proposed. It was derived from two defined base cell matrices. First, it captured a series of stereo images of markers moving in various directions through the object space. The world and image coordinates of markers were acquired, stored and used to create the two base cell matrices...
September 27, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30292168/the-role-of-the-secondary-phloem-during-the-development-of-the-grapevine-berry-shrivel-ripening-disorder
#11
Sara Crespo-Martínez, Mirosław Sobczak, Elżbieta Różańska, Astrid Forneck, Michaela Griesser
Berry Shrivel (BS) is a post-veraison physiological ripening disorder of grapevine berries. Its symptoms encompass low pH, reduced content of sugars and anthocyanins, and loss of turgor leading to berries shriveling. Evidence for the primary causes of BS is still speculative and anatomical studies are scarce. So far, anatomical studies have determined necrotic cells, degraded primary phloem cells and hardening of secondary phloem cells in the rachis of BS affected grapes. The picture is far from being complete...
September 27, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30312853/investigating-chemical-and-structural-instabilities-of-lead-halide-perovskite-induced-by-electron-beam-irradiation
#12
Xuanyu Chen, Zhiwei Wang
Chemical and structural instabilities of lead halide perovskites remain a major obstacle to their large-scale applications in photovoltaic solar cells and optoelectronic devices. Herein we report on a comparative investigation of electron beam irradiation effects on (all-inorganic) CsPbI3 and (organic-inorganic hybrid) CH3 NH3 PbI3 halide perovskite thin films by transmission electron microscopy. Upon continuous electron beam irradiation with high dose rates, CsPbI3 exhibits rather rapid morphological change and compositional degradation within irradiated regions, which are attributed to the radiolysis effect and probably assisted by electron beam heating and Coulomb forces...
September 22, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30265881/structural-analysis-of-single-nanoparticles-in-liquid-by-low-dose-stem-nanodiffraction
#13
Abdelali Khelfa, Caroline Byun, Jaysen Nelayah, Guillaume Wang, Christian Ricolleau, Damien Alloyeau
Liquid-cell TEM has enabled an interdisciplinary community of scientists to carry out atomic- / nano-scale studies of solid/liquid interfaces. Nevertheless, the restricted resolution of TEM in liquid media and the necessity to reduce the electron dose to avoid harmful radiolytic effects induced by the beam have limited the use of high resolution imaging to study the atomic structure of nanomaterials in liquid. Here we show that STEM nanodiffraction can be exploited in liquid-cell TEM experiments to overcome these two limitations...
September 17, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30265880/dynamic-behavior-of-nanoscale-liquids-in-graphene-liquid-cells-revealed-by-in-situ-transmission-electron-microscopy
#14
Jiwoong Yang, Sardar B Alam, Lei Yu, Emory Chan, Haimei Zheng
Recent advances in graphene liquid cells for in situ transmission electron microscopy (TEM) have opened many opportunities for the study of materials transformations and chemical reactions in liquids with high spatial resolution. However, the behavior of thin liquids encapsulated in a graphene liquid cell has not been fully understood. Here, we report real time TEM imaging of the nanoscale dynamic behavior of liquids in graphene nanocapillaries. Our observations reveal that the interfaces between liquid and gas bubble can fluctuate, leading to the generation of liquid nanodroplets near the interfaces...
September 17, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30388437/comparison-between-a-low-voltage-benchtop-electron-microscope-and-conventional-tem-for-number-size-distribution-of-nearly-spherical-shape-constituent-particles-of-nanomaterial-powders-and-colloids
#15
C Dazon, B Maxit, O Witschger
Nanomaterial powders and colloids are already a large industry and are expected to continue to grow rapidly. In the context of risk assessment associated with nanomaterials, characterization of nanoparticle size and morphology is required. Until now, the best method giving direct access to these parameters has been electron microscopy (EM), in particular, transmission electron microscopy (TEM). Although this method is widely used, several issues are highlighted such as cost, maintenance, sample representativity and damage for sensitive materials...
September 15, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30223177/phase-transition-and-hardness-evolution-of-a-ti-5al-5mo-1fe-1cr-alloy-subjected-to-isothermal-aging
#16
Zongyu Xie, Xiaoqin Ou, Song Ni, Min Song
In this paper, a Ti-5Al-5Mo-1Fe-1Cr alloy was isothermally aged at 500 °C for different periods. Phase transformation took place during the aging treatment. The phase transformation was studied by X-ray diffraction spectroscopy and transmission electron microscopy. The as-quenched sample was composed of matrix β (body-centered cubic structure) phase, ωath (hexagonal structure) phase and minor α (hexagonal close-packed structure) phase. The results showed that aging treatment promoted the β → ω → α phase transformations in the as-quenched alloys...
September 11, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30219738/preparation-of-surfaces-of-composite-samples-for-tip-based-micro-analyses-using-ion-beam-milling
#17
Jan Pinc, Marcela Dendisová, Kateřina Kolářová, Ondrej Gedeon, Marie Švecová, David Hradil, Janka Hradilová, Vilém Bartůněk
Ion beam milling, as a method of surface design for tip analytical techniques, was explored. A sample of clay, embedded in a resin, was treated by the ion beam and allowed AFM (a typical tip technique) to be successfully applied. The method is suitable for advanced tip analyses based on AFM, like TERS or SNOM, and for samples that are not possible to prepare by standard mechanical methods. The approach can be useful for characterisation of the surfaces of many different types of materials in versatile applications such as catalysis, corrosion science or advanced material characterisation...
September 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30300824/hole-free-phase-plate-tomography-for-materials-sciences-samples
#18
Misa Hayashida, Kai Cui, Amin Morteza Najarian, Richard McCreery, Neerushana Jehanathan, Chris Pawlowicz, Sohei Motoki, Masahiro Kawasaki, Yuji Konyuba, Marek Malac
We report, for the first time, the three dimensional reconstruction (3D) of a transistor from a microprocessor chip and roughness of molecular electronic junction obtained by electron tomography with Hole Free Phase Plate (HFPP) imaging. The HFPP appears to enhance contrast between inorganic materials and also increase the visibility of interfaces between different materials. We demonstrate that the degree of enhancement varies depending on material and thickness of the samples using experimental and simulation data...
September 7, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30219739/fast-finecut-grain-boundary-detection-in-microscopic-images-considering-3d-information
#19
Boyuan Ma, Xiaojuan Ban, Ya Su, Chuni Liu, Hao Wang, Weihua Xue, Yonghong Zhi, Di Wu
The inner structure of a material is called its microstructure. It stores the genesis of a material and determines all the physical and chemical properties. However, the microstructure is highly complex and numerous image defects such as vague or missing boundaries formed during sample preparation, which makes it difficult to extract the grain boundaries precisely. In this work, we address the task of grain boundary detection in microscopic image processing and develop a graph-cut based method called Fast-FineCut to solve the problem...
September 7, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/30199746/morphology-and-ultrastructure-of-the-infrabuccal-pocket-and-its-lining-epithelium-in-workers-of-ectomomyrmex-javanus-hymenoptera-formicidae
#20
Chu Wang, Johan Billen, Xingran Pan, Hong He
This paper describes the morphology and ultrastructure of the infrabuccal pocket in workers of the carnivorous ant Ectomomyrmex javanus, using SEM and TEM. The infrabuccal pocket is a flexible bag with a diameter of 330 ± 30 μm. In its anterior part, the pocket wall reaches a thickness of 24.45 ± 3.45 μm, which is thicker than the wall lining the rest of the infrabuccal pocket, where it measures 6.87 ± 1.12 μm. The epidermal cells of the wall form a thickened epithelium with a thickness of 10...
September 5, 2018: Micron: the International Research and Review Journal for Microscopy
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