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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/29028519/tem-observation-on-phase-separation-and-interfaces-of-laser-surface-alloyed-high-entropy-alloy-coating
#1
Zhaobing Cai, Xiufang Cui, Guo Jin, Zhe Liu, Yang Li, Meiling Dong
Phase separation is a common phenomenon in traditional alloys. Under the condition of appropriate undercooling, the segregation phenomenon can be also found in blue-chip high-entropy alloys (HEAs). In this work, the phase separation behavior and interfacial investigation of laser surface alloyed HEA coating with high content Ti were studied principally by transmission electron microscopy. The results show that crystal structure and elementary composition on both sides of the interface of coating/substrate are quite different, and the interfaces between different phases are incoherent or semi-coherent boundarys, resolved by high resolution transmission electron microscopy...
October 5, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28992458/quantitative-analysis-of-actin-filament-assembly-in-yeast-and-plant-by-live-cell-fluorescence-microscopy
#2
Joseph Jun Dao Tan, Zhiming Ma, Ying Xie, Liang Yang, Yansong Miao
Eukaryotic cells depend on a dynamic actin cytoskeleton to regulate many conserved intracellular events such as endocytosis, morphogenesis, polarized cell growth, and cytokinesis (Engqvist-Goldstein and Drubin, 2003; Salbreux et al., 2012; Pruyne et al., 2004; Pollard, 2010). These activities depend on a precise and well-organized spatiotemporal actin assembly that involves many conserved processes found in eukaryotic cells ranging from a unicellular organism, such as yeast, to multicellular organisms, such as plants and human...
September 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28992457/a-parameter-for-the-assessment-of-the-segmentation-of-tem-tomography-reconstructed-volumes-based-on-mutual-information
#3
Ana Okariz, Teresa Guraya, Maider Iturrondobeitia, Julen Ibarretxe
A method is proposed and verified for selecting the optimum segmentation of a TEM reconstruction among the results of several segmentation algorithms. The selection criterion is the accuracy of the segmentation. To do this selection, a parameter for the comparison of the accuracies of the different segmentations has been defined. It consists of the mutual information value between the acquired TEM images of the sample and the Radon projections of the segmented volumes. In this work, it has been proved that this new mutual information parameter and the Jaccard coefficient between the segmented volume and the ideal one are correlated...
September 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28968548/photosynthetic-and-ultrastructural-responses-of-ulva-australis-to-zn-stress
#4
REVIEW
D R Farias, E Schmidt, C Simioni, Z L Bouzon, C L Hurd, R S Eriksen, C K Macleod
This research evaluated the effect of zinc (Zn) on the ultrastructure and the photosynthetic efficiency of a common green alga. Ulva australis was grown in the laboratory for 7days under a range of different Zn concentrations (0, 25, 50 and 100μgL(-1)). Growth rate (Gr), photosynthetic efficiency (Fv/Fm and ETRmax), photosynthetic pigments, and metal accumulation were measured. Samples of 1mm length were taken to analyse the effect of Zn on the ultrastructure using transmission electron microscopy (TEM) and cytochemical responses (TB-O and PAS) were evaluated by light microscopy (LM)...
September 28, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28972922/transmission-kikuchi-diffraction-versus-electron-back-scattering-diffraction-a-case-study-on-an-electron-transparent-cross-section-of-twip-steel
#5
Azdiar A Gazder, Khalil I Elkhodary, Mitchell J B Nancarrow, Ahmed A Saleh
The present case study compares transmission Kikuchi diffraction (TKD) with electron back-scattering diffraction (EBSD) on the same area of an electron transparent cross-section of a twinning induced plasticity steel. While TKD expectedly provides better clarity of internal defect substructures in the band contrast map, EBSD returns orientation data that approaches the quality of the TKD map. This was rationalised by Monte Carlo simulations of the electron energy spreads, which showed that due to the geometry-based compromises associated with adapting a conventional EBSD detector (which is off-axis with respect to the incident electron beam) to TKD, a broadening in the electron energy distribution of the forward-scattered electrons collected on the detector phosphor screen, is unavoidable...
September 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28946025/effects-of-thermal-and-deformation-on-martensitic-transformation-and-magnetic-properties-in-fe-17-mn-4-5-x-x-co-and-mo-alloys
#6
REVIEW
Osman Armağan, Uğur Sarı, Çağrı Yücel, Talip Kırındı
In this study, the Co and Mo addition to Fe-Mn based alloys was investigated to observe its effect on martensitic phase transformation. Two types of martensite structure occurred from the surface observations made by SEM for Co-added and Mo-added alloys morphologically in austenite grain. With the help of TEM studies, it was understood that these martensite structures are ε (h.c.p.) and α' (b.c.c.). TEM investigations showed that the orientation relationship between γ (f.c.c.) and ε phases corresponds to Shoji-Nishiyama type and between γ and α' phases corresponds to Kurdjumov-Sachs (K-S) type...
September 20, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29031165/characterization-and-morphological-analysis-of-individual-aerosol-of-pm10-in-urban-area-of-lucknow-india
#7
Sushil Kumar Bharti, Dhananjay Kumar, Sangeeta Anand, Poonam, Shymal Chandra Barman, Narendra Kumar
Airborne particulate matters were collected during the period of October 2015 to September 2016 in Lucknow at different sampling sites. The annual mean concentration of particulate matter was found to be relatively higher than the limits prescribed by National ambient air quality standards (NAAQS), United State Environmental Protection Agency (USEPA) and World Health Organization (WHO). Particulate matters were studied for morphological analysis, elemental composition and functional group variability with the help of Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM-EDS) followed by Fourier Transform Infrared spectroscopy (FTIR)...
September 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28942370/carbides-and-possible-hydrogen-irreversible-trapping-sites-in-ultrahigh-strength-round-steel
#8
X Y Cheng, H Li, X B Cheng
The carbides in ultrahigh strength round steel have been investigated by using laser-assisted atom probe tomography (APT) and high resolution transmission electron microscopy (HRTEM) in this paper. Two kinds of carbides are found and one is iron carbide M6C, where carbide formation elements Cr, Mn and Mo replace partial Fe, while the other is niobium carbide MC, where M includes V and Mo besides Nb. These two carbides, due to their different evaporation field, have various densities in reconstructed image of APT...
September 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28946024/automatic-system-for-electron-tomography-data-collection-in-the-ultra-high-voltage-electron-microscope
#9
Meng Cao, Ryuji Nishi, Fang Wang
In this study, we report an automatic system for collection of tilt series for electron tomography based on the ultra-HVEM in Osaka University. By remotely controlling the microscope and reading the observation image, the system can track the field of view and do focus in each tilt angle. The automatic tracking is carried out with an image matching technique based on normalized correlation coefficient. Auto focus is realized by the optimization of image sharpness. A toolkit that can expand the field of view with technique of image stitching is also developed...
September 12, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28915413/transmission-electron-microscopy-studies-and-modeling-of-3d-reciprocal-space-of-%C3%AF-forming-alloy
#10
Alphy George, Vinayak Sharma, R Divakar, M Sabeena, E Mohandas
Initial stage of ω phase formation and associated anomalous features that appear in diffraction patterns of a metastable β transition metal alloy have been investigated in this study with the aid of transmission electron microscopy, simulation and modeling. The paper explores discrete features that emerge in selected area diffraction patterns of quenched Ti-15wt%Mo alloy and analyzes the correlation between ω reflections and diffuse arcs by considering all variants of ω phase as per the formation kinetics of ω phase in β matrix while quenching...
September 12, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28942369/slanted-support-window-based-stereo-matching-for-surface-reconstruction-of-microscopic-samples
#11
Ahmadreza Baghaie, Chi Zhang, Ali Bakhshinejad, Heather A Owen, Hongyang Chao, Roshan M D'Souza, Zeyun Yu
This work is to address the limitations of 2D Scanning Electron Microscopy (SEM) micrographs in providing 3D topographical information necessary for various types of analysis in biological and biomedical sciences as well as mechanical and material engineering by investigating modern stereo vision methodologies for 3D surface reconstruction of microscopic samples. To achieve this, micrograph pairs of the microscopic samples are acquired by utilizing an SEM equipped with motor controlled specimen stage capable of precise translational, rotational movements and tilting of the specimen stage...
September 8, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28917581/an-engineering-insight-into-the-relationship-of-selective-cytoskeletal-impairment-and-biomechanics-of-hela-cells
#12
Daniele Borin, Luca Puzzi, Valentina Martinelli, Matteo Cibinel, Romano Lapasin, Orfeo Sbaizero
It is widely accepted that the pathological state of cells is characterized by a modification of mechanical properties, affecting cellular shape and viscoelasticity as well as adhesion behaviour and motility. Thus, assessing these parameters could represent an interesting tool to monitor disease development and progression, but also the effects of drug treatments. Since biomechanical properties of cells are strongly related to cytoskeletal architecture, in this work we extensively studied the effects of selective impairments of actin microfilaments and microtubules on HeLa cells through force-deformation curves and stress relaxation tests with atomic force microscopy...
September 8, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28922627/distribution-patterns-and-morphology-of-sensilla-on-the-antennae-of-plutella-xylostella-l-a-scanning-and-transmission-electron-microscopic-study
#13
Xi-Zhong Yan, Cai-Ping Deng, Jiao-Xin Xie, Lan-Jun Wu, Xue-Jun Sun, Chi Hao
The antennal morphology, types of antennal sensilla, fine structures and distributions of the sensilla in Plutella xylostella L. (Lepidoptera: Plutellidae) were studied by scanning (SEM) and transmission (TEM) electron microscopy. The antenna, scape, pedicel and flagellum were all longer in males than in females. A total of seventeen types of sensilla were identified on the antennae: trichodea (two subtypes), basiconica, coeloconica (three subtypes), Böhm's bristles (two subtypes), styloconica (two subtypes), squamiformia, auricillica, furcatea (three subtypes), cupuliform organs and terminal sensory pegs...
September 6, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28892779/a-new-method-for-measuring-the-contact-angles-from-digital-images-of-liquid-drops
#14
M Mirzaei
The drop hitting a solid surface may be symmetric or asymmetric, which depends on the surface texture and external force orientations. The accurate measurement of the contact angle is of fundamental importance for the purpose of scientific research, while having a substantial role in a wide range of practical applications. This paper presents a new image processing based method, as a computational scheme to measure the inclination angle of apparent edge curves in digital images. The main concept of the scheme is the emulation of a moving goniometer mask coupled with a Gaussian weighted function, which does not require edge fitting with analytic curves for the angle calculation...
September 5, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28888130/chiral-supramolecular-order-revealed-during-the-formation-of-calf-thymus-and-phage-dna-crystals
#15
Benedicto de Campos Vidal, Maria Luiza S Mello
The control of DNA packaging has been reported to be dependent on an ordered liquid-crystalline state. However, the textural characteristics that are typical of crystals and that resemble mesophases have not been reported for highly polymerized or even shorter types of DNA filaments under in vitro conditions that favor crystallization. Because DNA crystals are expected to exhibit particular textural optical anisotropies, pure and highly polymerized calf thymus DNA and simpler λ phage DNA were crystallized from solution drops and were analyzed using high-performance polarization microscopy with and without differential interference contrast (DIC) optics...
September 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28869874/effects-of-micro-structure-on-aerodynamics-of-coccinella-septempunctata-elytra-ladybird-in-forward-flight-as-assessed-via-electron-microscopy
#16
Jinwu Xiang, Kai Liu, Daochun Li, Jianxun Du
The effects of micro-structure on aerodynamics of Coccinella septempunctata (Coleoptera: Coccinellidae) elytra in forward flight were investigated. The micro-structure was examined by a scanning electron microscope and a digital microscope. Based on the experimental results, five elytron models were constructed to separately investigate the effects of the camber and the local corrugation in both leading edge and trailing edge on aerodynamics. Computational fluid dynamic simulations of five elytron models were conducted by solving the Reynolds-Averaged Navier-Stokes equations with the Reynolds number of 245...
September 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28889072/the-haemocytes-of-the-colonial-aplousobranch-ascidian-diplosoma-listerianum-structural-cytochemical-and-functional-analyses
#17
Francesca Cima, Anna Peronato, Loriano Ballarin
Diplosoma listerianum is a colonial aplousobranch ascidian of the family Didemnidae that is native to the northeast Atlantic and exhibits a cosmopolitan distribution in temperate waters. It lacks a shared colonial circulation crossing the tunic, and the zooids are connected only by the common tunic. In the present study, the haemocytes of this ascidian were analysed via light and electron microscopy. Their phagocytic and enzymatic activities, staining and immunostaining properties, and lectin affinity were examined with various classical methods reconsidered and modified for small marine invertebrates...
August 30, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28869875/ultrastructure-and-morphometric-features-of-epididymal-epithelium-in-desmodus-rotundus
#18
Mariana Moraes de Castro, Wagner Gonzaga Gonçalves, Stéphanie Asséf Millen Valente Teixeira, Maria do Carmo Queiroz Fialho, Felipe Couto Santos, Jerusa Maria Oliveira, José Eduardo Serrão, Mariana Machado-Neves
The blood-feeding behavior of Desmodus rotundus made this bat a potential vector of rabies virus and a public health issue. Consequently, the better understanding of its reproductive biology becomes valuable for the development of methods to control its population. In this study, we described morphological aspects of epithelial cells in D. rotundus' epididymis using light and transmission electron microscopy methods. The duct compartment was the main component of initial segment (83%), caput (90%), corpus (88%) and cauda (80%) regions...
August 26, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28858637/compensation-of-the-magnetic-force-imaging-by-scanning-directions
#19
Jinyun Liu, Miao Yu, Yingmin Qu, Wenxiao Zhang, Yinxue Fan, Zhengxun Song, Renxi Qiu, Dayou Li, Zuobin Wang
It was found that the results of magnetic force microscope (MFM) imaging were different with the probe scanning directions. This paper studied the effect of scanning directions on the MFM imaging, and a method for the distortion compensation was proposed to reduce the errors. In the study, three different scanning directions with the angles of 0°, 45° and 90° were used to measure the magnetic domain structures distributions of magnetic sample. The experimental results have shown that the scanning direction parallel to the magnetic domain structure will cause a minimum phase shift difference and lead to a structure distortion...
August 26, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28830057/histopathological-and-ultrastructural-changes-experimentally-induced-by-bee-venom-in-seminiferous-epithelium-via-structural-functional-alteration-of-sertoli-cells
#20
Adrian Florea, Constantin Puică, Sami Hamed, Mariana Tilinca, Horea Matei
We tested here the ability of bee venom (BV) to interfere with spermatogenesis in rats in two experimental conditions. The histopathological changes were assessed with brightfield microscopy using a novel staining technique, based on methylene blue, orange G and ponceau xylidine. Transmission electron microscopy was also used to identify fine subcellular changes. BV injection for 30days in daily doses of 700μg BV/kg resulted in reducing testicular weight, along with significant larger diameters of seminiferous tubules and reduced number of Sertoli cells (SCs)...
August 16, 2017: Micron: the International Research and Review Journal for Microscopy
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