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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/29772475/impact-of-cu-ii-doping-on-the-vulnerability-of-escherichia-coli-atcc-10536-revealed-by-atomic-force-microscopy
#1
Wasia Rasheed, Samina Perveen, Ghulam Mustafa, Muhammad Raza Shah, Shakil Ahmed, Sami Uzzaman
E. coli strain is a gram-negative bacterium known to induce both extra-intestinal infections and intestinal infections. For survival of microbes, metal intake and accessibility should be according to their physiological requirements. Peculiarly, copper homeostasis is critical for E. coli survival and growth. Therefore in this study, an extensive work is conducted to investigate the impact of Cu(II)-doping on the susceptibility of Escherichia coli ATCC 10536 against Cu(II)-selective Cefaclor-silver nanoconjugates (i...
May 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29753176/in-situ-mechanical-test-of-dragonfly-wing-veins-and-their-crack-arrest-behavior
#2
Zhihui Zhang, Lan Zhang, Zhenglei Yu, Jingjing Liu, Xiujuan Li, Yunhong Liang
In natural biological systems, many insects in complex environments exhibit exemplary mechanical properties. Dragonfly wings are light and strong enough to withstand wind loading. Their rigid veins play supporting and strengthening roles to enhance resistance to fatigue. To explore the effect of veins on arresting cracking in the wing, the costa, subcosta, radius R1, and two areas of dragonfly hind wings were samples for in situ tensile tests. The fracture process of the samples was observed with a high-speed camera and a scanning electron microscope...
May 5, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29738917/analysis-and-comparison-of-focused-ion-beam-milling-and-vibratory-polishing-sample-surface-preparation-methods-for-porosity-study-of-u-mo-plate-fuel-for-research-and-test-reactors
#3
Bjorn Westman, Brandon Miller, Jan-Fong Jue, Assel Aitkaliyeva, Dennis Keiser, James Madden, Julie D Tucker
Uranium-Molybdenum (U-Mo) low enriched uranium (LEU) fuels are a promising candidate for the replacement of high enriched uranium (HEU) fuels currently in use in a high power research and test reactors around the world. Contemporary U-Mo fuel sample preparation uses focused ion beam (FIB) methods for analysis of fission gas porosity. However, FIB possess several drawbacks, including reduced area of analysis, curtaining effects, and increased FIB operation time and cost. Vibratory polishing is a well understood method for preparing large sample surfaces with very high surface quality...
May 2, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29734020/ultrastructural-and-mechanical-changes-in-tubular-epithelial-cells-by-angiotensin-ii-and-aldosterone-as-observed-with-atomic-force-microscopy
#4
Fu-Shi Quan, Kyung Hwan Jeong, Gi-Ja Lee
Tubular epithelial cells (TECs) play an important pathophysiological role in the promotion of renal fibrosis. Quantitative analysis of the mechanical changes in TECs may be helpful in evaluating novel pharmacological strategies. Atomic force microscopy (AFM) is a common nanotechnology tool used for imaging and measuring interaction forces in biological systems. In this study, we used AFM to study ultrastructural and mechanical changes in TECs mediated by the renin-angiotensin-aldosterone system. We quantitatively analyzed changes in the mechanical properties of TECs using three extrinsic factors, namely, chemical fixation, angiotensin II (AT II), and aldosterone (AD)...
May 2, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29772476/fate-of-nuclear-material-during-subsequent-steps-of-the-kinetin-induced-pcd-in-apical-parts-of-vicia-faba-ssp-minor-seedling-roots
#5
Andrzej Kaźmierczak, Kamila Soboska
In animals during apoptosis, the best examined type of programmed cell death (PCD), three main phases are distinguished: (i) specification (signaling), (ii) killing and (iii) execution one. It has bean postulated that plant PCD also involves three subsequent phases: (i) transmission of death signals to cells (signaling), (ii) initiation of killing processes and (iii) destruction of cells. One of the most important hallmarks of animal and plant PCD are those regarding nucleus, not thoroughly studied in plants so far...
April 30, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29730507/temperature-distribution-of-wedge-shaped-specimen-in-tem
#6
Lu Chen, Yong Wang, Ze Zhang
Understanding the electron irradiation effects, especially the heating effect, is very important to the in-situ TEM experiments. A model is established to describe the temperature distribution of FIB prepared wedge-shaped specimen induced by electron irradiation, and a Cu2 Se specimen is presented to estimate the temperature distribution inside the observed area under different dose rates in an in-situ TEM heating experiments. The result indicates that in normal situations, the electron irradiation caused temperature inhomogeneity inside the observed area is ignorable...
April 25, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29727775/spectral-method-as-a-tool-to-examine-microtextures-of-quartz-sand-sized-grains
#7
REVIEW
Sylwia Szerakowska, Barbara Woronko, Maria J Sulewska, Edward Oczeretko
Well-rounded grains extracted from aeolian and subaqueous environments were analysed to determine a quantitative parameter describing the microtopographic surface properties of sand-sized quartz grains, expressed as a degree of smoothness or as an inverse roughness. To this end, the spectral method was used to calculate their fractal dimension values. Fractal characteristics and spectral fractal dimension (DS ) were determined for a scanning electron microscope (SEM) image (560 × 560 pixels) obtained for each study grain...
April 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29715620/the-innovation-of-cryo-sem-freeze-fracturing-methodology-demonstrated-on-high-pressure-frozen-biofilm
#8
Kamila Hrubanova, Jana Nebesarova, Filip Ruzicka, Vladislav Krzyzanek
In this study we present an innovative method for the preparation of fully hydrated samples of microbial biofilms of cultures Staphylococcus epidermidis, Candida parapsilosis and Candida albicans. Cryo-scanning electron microscopy (cryo-SEM) and high-pressure freezing (HPF) rank among cutting edge techniques in the electron microscopy of hydrated samples such as biofilms. However, the combination of these techniques is not always easily applicable. Therefore, we present a method of combining high-pressure freezing using EM PACT2 (Leica Microsystems), which fixes hydrated samples on small sapphire discs, with a high resolution SEM equipped with the widely used cryo-preparation system ALTO 2500 (Gatan)...
April 22, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29705338/correcting-sample-drift-using-fourier-harmonics
#9
G Bárcena-González, M P Guerrero-Lebrero, E Guerrero, D F Reyes, V Braza, A Yañez, B Nuñez-Moraleda, D González, P L Galindo
During image acquisition of crystalline materials by high-resolution scanning transmission electron microscopy, the sample drift could lead to distortions and shears that hinder their quantitative analysis and characterization. In order to measure and correct this effect, several authors have proposed different methodologies making use of series of images. In this work, we introduce a methodology to determine the drift angle via Fourier analysis by using a single image based on the measurements between the angles of the second Fourier harmonics in different quadrants...
April 21, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29689433/new-airtight-transfer-box-for-sem-experiments-application-to-lithium-and-sodium-metals-observation-and-analyses
#10
Nicolas Stephant, Rabeb Grissa, Fanch Guillou, Mickaël Bretaudeau, Yann Borjon-Piron, Jacques Guillet, Philippe Moreau
The surface of some materials reacts very quickly on contact with air, either because it is oxidized or because it gets humidity from the air. For the sake of original surface observation by scanning electron microscopy (SEM), we conceived an airtight transfer box to keep the samples under vacuum from the place of manufacturing to the SEM chamber. This object is designed to fit in all the models of SEM including those provided with an airlock chamber. The design is voluntarily simplified to allow the manufacturing of the object by a standard mechanical workshop...
April 18, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29689432/a-fluorescence-in-situ-staining-method-for-investigating-spores-and-vegetative-cells-of-clostridia-by-confocal-laser-scanning-microscopy-and-structured-illuminated-microscopy
#11
P D'Incecco, L Ong, S Gras, L Pellegrino
Non-pathogenic spore-forming Clostridia are of increasing interest due to their application in biogas production and their capability to spoil different food products. The life cycle for Clostridium includes a spore stage that can assist in survival under environmentally stressful conditions, such as extremes of temperature or pH. Due to their size, spores can be investigated by a range of microscopic techniques, many of which involve sample pre-treatment. We have developed a quick, simple and non-destructive fluorescent staining procedure that allows a clear differentiation between spores and vegetative cells and effectively stains spores, allowing recovery and tracking in subsequent experiments...
April 18, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29665457/three-dimensional-geometrical-and-topological-characteristics-of-grains-in-conventional-and-grain-boundary-engineered-316l-stainless-steel
#12
Tingguang Liu, Shuang Xia, Bangxin Zhou, Qin Bai, Gregory S Rohrer
The three-dimensional microstructures of a conventional 316L stainless steel and the same material after grain boundary (GB) engineering have been measured by serial sectioning coupled with electron backscatter diffraction mapping. While it is well known that GB engineered materials are differentiated from conventional materials because of the proportion of coincidence site lattice boundaries, the size of their twin-related domains, and their reduced random boundary connectivity, this work provides a quantitative comparison of the geometrical and topological characteristics of grains in 316L stainless steel before and after GB engineering...
April 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29649773/optimization-study-of-direct-morphology-observation-by-cold-field-emission-sem-without-gold-coating
#13
Dan He, Cheng Fu, Zhigang Xue
Gold coating is a general operation that is generally applied on non-conductive or low conductive materials, during which the morphology of the materials can be examined by scanning electron microscopy (SEM). However, fatal deficiencies in the materials can result in irreversible distortion and damage. The present study directly characterized different low conductive materials such as hydroxyapatite, modified poly(vinylidene fluoride) (PVDF) fiber, and zinc oxide nanopillar by cold field emission scanning electron microscopy (FE-SEM) without a gold coating...
April 6, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29635074/stem-and-apt-characterization-of-scale-formation-on-a-la-hf-ti-doped-nicral-model-alloy
#14
Kinga A Unocic, Yimeng Chen, Dongwon Shin, Bruce A Pint, Emmanuelle A Marquis
A thermally grown scale formed on a cast NiCrAl model alloy doped with lanthanum, hafnium, and titanium was examined after isothermal exposure at 1100 °C for 100 h in dry flowing O2 to understand the dopant segregation along scale grain boundaries. The complex scale formed on the alloy surface was composed of two types of substrates: phase-dependent, thin (<250 nm) outer layers and a columnar-grained ∼3.5 μm inner alumina layer. Two types of oxides formed between the inner and outer scale layers: small (3-15 nm) La2 O3 and larger (≤50 nm) HfO2 oxide precipitates...
April 6, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29614427/petrographic-characterization-to-build-an-accurate-rock-model-using-micro-ct-case-study-on-low-permeable-to-tight-turbidite-sandstone-from-eocene-shahejie-formation
#15
Muhammad Jawad Munawar, Chengyan Lin, Veerle Cnudde, Tom Bultreys, Chunmei Dong, Xianguo Zhang, Wesley De Boever, Muhammad Aleem Zahid, Yuqi Wu
Pore scale flow simulations heavily depend on petrographic characterizing and modeling of reservoir rocks. Mineral phase segmentation and pore network modeling are crucial stages in micro-CT based rock modeling. The success of the pore network model (PNM) to predict petrophysical properties relies on image segmentation, image resolution and most importantly nature of rock (homogenous, complex or microporous). The pore network modeling has experienced extensive research and development during last decade, however the application of these models to a variety of naturally heterogenous reservoir rock is still a challenge...
March 26, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29604549/challenges-in-tem-sample-preparation-of-solvothermally-grown-cuins-2-films
#16
Anna Frank, Rasa Changizi, Christina Scheu
Transmission electron microscopy (TEM) is a widely used tool to characterize materials. The required samples need to be electron transparent which should be achieved without changing the microstructure. This work describes different TEM sample preparation techniques of nanostructured CuInS2 thin films on fluorine-doped tin oxide substrates, synthesized solvothermally using l-cysteine as sulfur source. Focused ion beam lamellae, conventional cross section samples and scratch samples have been prepared and investigated...
March 23, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29614428/involvement-of-autophagy-and-apoptosis-and-lipid-accumulation-in-sclerotial-morphogenesis-of-morchella-importuna
#17
Peixin He, Ke Wang, Yingli Cai, Xiaolong Hu, Yan Zheng, Junjie Zhang, Wei Liu
Sclerotial formation is a key phase of the morel life cycle and lipids have been recorded as the main cytoplasmic reserves in sclerotia of Morchella fungi without any experimental verification. In this study, the ultrastructural features of the undifferentiated mycelia stage (MS) and three main sclerotial differentiation states (sclerotial initial [SI], sclerotial development [SD] and sclerotial maturation [SM]) were compared by transmission electron microscopy. The nature of the energy-rich substance in hypha and sclerotium of Morchella importuna was qualitatively investigated by confocal laser scanning microscopy and quantitatively studied by extraction of lipids...
March 21, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29609093/histological-and-electron-microscopy-observations-on-the-testis-and-spermatogenesis-of-the-butterfly-dione-juno-cramer-1779-and-agraulis-vanillae-linnaeus-1758-lepidoptera-nymphalidae
#18
Isabelle Pereira Mari, Adriana Aparecida Sinópolis Gigliolli, Satiko Nanya, Ana Luiza de Brito Portela-Castro
Lepidopteran species present an interesting case of sperm polymorphism and testicular fusion. The study of these features are of great importance in understanding the reproductive biology of these insects, especially in the case of those considered pests. Dione juno and Agraulis vanillae stand out as the most important pests of passion fruit (Passiflora sp.) crops in Brazil. Therefore, the objective of the present study was to characterize the testes and germ cells of Dione juno and Agraulis vanillae at different life stages, using light microscopy and scanning and transmission electron microscopy, to understand the maturation mechanisms of the male gametes in these species...
March 20, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29550673/high-speed-low-dose-analytical-electron-microscopy-with-dynamic-sampling
#19
Karl A Hujsak, Eric W Roth, William Kellogg, Yue Li, Vinayak P Dravid
Technological advances in electron microscopy, particularly improved detectors and aberration correctors, have led to higher throughput and less invasive imaging of materials and biological structures by enhancing signal-to-noise ratios at lower electron exposures. Analytical methods, such as electron energy loss spectroscopy (EELS) and energy dispersive x-ray spectrometry (EDS), have also benefitted and offer a rich set of local elemental and bonding information with nano-or atomic resolution. However, spatially resolved spectrum imaging with EELS and EDS continue to be difficult to scale due to limited detector collection angles or high signal background, requiring hours or even days for full maps...
March 10, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29549780/one-pot-single-step-to-label-microtubule-with-mpa-capped-cdte-quantum-dots
#20
Daniel Oliveira, Fernando Menegatti de Melo, Henrique E Toma
The kinesin-microtubule pair has attracted much attention in recent years due to their promising use in the development of synthetic nanotransport machines. One of the challenges to study such system is how to observe the motility of either kinesin or microtubule. The usual technique for observation of the molecular machinery pair is by fluorescence microscopy, where fluorescent probes are bound to one, or both species, through a biotin-avidin linker. Here, the use of mercaptopropionic acid (MPA)-capped cadmium telluride (CdTe) quantum dots (QDs) as a direct fluorescent labeling agent to microtubule biomolecules is reported...
March 10, 2018: Micron: the International Research and Review Journal for Microscopy
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