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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/28076759/quantitative-in-situ-tem-nanotensile-testing-of-single-crystal-ni-facilitated-by-a-new-sample-preparation-approach
#1
Vahid Samaeeaghmiyoni, Hosni Idrissi, Jonas Groten, Ruth Schwaiger, Dominique Schryvers
Twin-jet electro-polishing and Focused Ion Beam (FIB) were combined to produce small size Nickel single crystal specimens for quantitative in-situ nanotensile experiments in the transmission electron microscope. The combination of these techniques allows producing samples with nearly defect-free zones in the centre in contrast to conventional FIB-prepared samples. Since TEM investigations can be performed on the electro-polished samples prior to in-situ TEM straining, specimens with desired crystallographic orientation and initial microstructure can be prepared...
December 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28063337/laser-assisted-atom-probe-tomography-of-ti-tin-films-deposited-on-si
#2
N A Sanford, P T Blanchard, R White, M R Vissers, D R Diercks, A V Davydov, D P Pappas
Laser-assisted atom probe tomography (L-APT) was used to examine superconducting TiN/Ti/TiN trilayer films with nominal respective thicknesses of 5/5/5 (nm). Such materials are of interest for applications that require large arrays of microwave kinetic inductance detectors. The trilayers were deposited on Si substrates by reactive sputtering. Electron energy loss microscopy performed in a scanning transmission electron microscope (STEM/EELS) was used to corroborate the L-APT results and establish the overall thicknesses of the trilayers...
December 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28049098/growth-morphology-and-special-diffraction-characteristics-of-multifaceted-gold-nanoparticles
#3
Manish Kumar Singh, Bratindranath Mukherjee, Rajiv Kumar Mandal
This paper deals with morphology of synthesized nano gold particles through seed based growth. Various types of morphologies have been observed. They primarily relate to decahedral and truncated tetrahedral shapes. Their relative abundance is dependent on temporal evolution of nanoparticles. These shapes are understood to have evolved from a seed having symmetry that is a supergroup of the point groups possessed by these nanoparticles. The usual pentagonal twinned morphologies observed under High Resolution Transmission Electron Microscopy have displayed two distinct types of interfaces...
December 18, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28027486/differential-expression-of-the-nucleolar-protein-fibrillarin-during-mammalian-spermatogenesis-and-its-probable-association-with-chromatoid-body-components
#4
Larissa Fiamengui de Pauli, Elisa Gomes Santos, Fernanda Pazotti Daher Arcangelo, Wilson Aparecido Orcini, Rita Luiza Peruquetti
Chromatoid body (CB) is a cytoplasmic structure of male germ cells that has been indicated as having a role in the RNA and protein storage for the final differentiation of spermatozoa. Recent studies have indicated that some of these macromolecular complex components have nucleolar origin. The aims of the present study were to monitor the expression of fibrillarin nucleolar protein in mammalian seminiferous tubules at different stages of the spermatogenic cycle; to check fibrillarin distribution during the CB assembly; and also its interaction with two well-known CB markers (MIWI and HSP70)...
December 14, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28011347/ebsd-and-electron-channeling-study-of-anomalous-slip-in-oligocrystals-of-high-chromium-ferritic-stainless-steel
#5
Zheng-Wen Hsiao, Ting-Yi Wu, Delphic Chen, Jui-Chao Kuo, Dong-Yih Lin
In the present study, electron backscatter diffraction (EBSD) and electron channeling contrast imaging (ECCI) techniques were applied to investigate the deformation pattern of coarse ferrite grains after being subjected to 3%, 6%, and 10% tensile deformation. Oligocrystals of Crofer(®) 22H ferritic steel were obtained as experimental material at 1075°C for 22min annealing. Using kernel average misorientation (KAM) mapping obtained from EBSD, possible slip planes are (110), (101), (12-1) and (32-1) in grain A; (0-11), (-101), (-112), (1-21) in grain B; and (0-11), (1-21) and (11-2) in grain C...
December 12, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28013144/effect-of-temperature-on-beam-damage-of-asbestos-fibers-in-the-transmission-electron-microscope-tem-at-100kv
#6
Joannie Martin, Martin Beauparlant, Sébastien Sauvé, Gilles L'Espérance
Damage to asbestos fibers by the transmission electron microscope (TEM) electron beam is a known limitation of this powerful method of analysis. Although it is often considered only in terms of loss of crystallinity, recent studies have shown that the damage may also change the elemental composition of fibers, thus causing significant identification errors. In this study, the main objective was to assess whether temperature is a factor influencing damage to asbestos fibers and, if so, how it can be used to minimize damage...
November 24, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27960108/a-technique-for-evaluating-bone-ingrowth-into-3d-printed-porous-ti6al4v-implants-accurately-using-x-ray-micro-computed-tomography-and-histomorphometry
#7
Anders Palmquist, Furqan A Shah, Lena Emanuelsson, Omar Omar, Felicia Suska
This paper investigates the application of X-ray micro-computed tomography (micro-CT) to accurately evaluate bone formation within 3D printed, porous Ti6Al4V implants manufactured using Electron Beam Melting (EBM), retrieved after six months of healing in sheep femur and tibia. All samples were scanned twice (i.e., before and after resin embedding), using fast, low-resolution scans (Skyscan 1172; Bruker micro-CT, Kontich, Belgium), and were analysed by 2D and 3D morphometry. The main questions posed were: (i) Can low resolution, fast scans provide morphometric data of bone formed inside (and around) metal implants with a complex, open-pore architecture?, (ii) Can micro-CT be used to accurately quantify both the bone area (BA) and bone-implant contact (BIC)?, (iii) What degree of error is introduced in the quantitative data by varying the threshold values?, and (iv) Does resin embedding influence the accuracy of the analysis? To validate the accuracy of micro-CT measurements, each data set was correlated with a corresponding centrally cut histological section...
November 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27974253/v-shaped-pits-in-hvpe-grown-gan-associated-with-columnar-inversion-domains-originating-from-foreign-particles-of-%C3%AE-si3n4-and-graphitic-carbon
#8
Tohoru Matsubara, Yusho Denpo, Narihito Okada, Kazuyuki Tadatomo
The v-shaped pits (so-called V-pits) observed in hydride-vapor-phase-epitaxy-grown GaN and associated with the columnar inversion domains originating from foreign particles were investigated. The inversion domains on the front and back surfaces of the test sample were recognized after chemical mechanical polishing. It was found that the V-pits originate from the columnar inversion domains. The inversion domains, in turn, arise from the particles that exist on a low-temperature GaN buffer layer on sapphire substrate...
November 18, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27866100/tem-illumination-settings-study-for-optimum-spatial-resolution-and-indexing-reliability-in-crystal-orientation-mappings
#9
A Valery, A Pofelski, L Clément, F Lorut, E F Rauch
The spatial resolution and the indexing quality obtained with an automated orientation and phase mapping tool are analyzed for different Transmission Electron Microscope (TEM) illumination settings. The electron probe size and convergence angle are studied for two TEM configuration modes referred as microprobe and nanoprobe modes. Using a 10μmC2 aperture in a FEI Tecnai F20 (S)TEM, the nanoprobe mode is used to get a small convergent electron beam while the microprobe mode provides a nearly parallel illumination at the cost of a larger probe size...
November 9, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27866099/how-to-image-cell-adhesion-on-soft-polymers
#10
Silke Seyock, Vanessa Maybeck, Andreas Offenhäusser
Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex(®)) and prepared for electron microscopy. The samples were treated with osmium tetroxide (OsO4) and uranylacetate (UrAc). Best results can be achieved when the polymers were coated with a thin iridium layer before the cell culture. This was done to emphasize the usage of soft polymers as supports for implant electrodes...
November 9, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27855318/a-method-for-measuring-the-three-dimensional-orientation-of-cortical-canals-with-implications-for-comparative-analysis-of-bone-microstructure-in-vertebrates
#11
Isaac Vorster Pratt, David Michael Lane Cooper
The orientation of vascular canals in cortical bone can reveal information about the growth rate and loading environment of a bone. For example, in birds it has been proposed that a high proportion of circumferential canals (a laminar cortex) is related to fast growth or torsional loading related to active flight. In this paper we present a method to measure the three dimensional (3D) orientation of vascular canals. Image data are obtained from micro-CT and two angles are measured: phi, determining how longitudinal a canal is; and theta, determining whether a canal is radial or circumferential...
November 2, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27846432/functionalized-nanoparticles-enable-tracking-the-rapid-entry-and-release-of-doxorubicin-in-human-pancreatic-cancer-cells
#12
Maheshika P Arachchige, Suvra S Laha, Akshata R Naik, Kenneth T Lewis, Ratna Naik, Bhanu P Jena
Efficient drug delivery is critical to therapy. Using electron microscopy, X-ray, and light microscopy, we have characterized functionalized superparamagnetic iron oxide (SPIO) nanoparticles, and determined their ability for rapid entry and release of the cancer drug doxorubicin in human pancreatic cancer cells. Dextran-coated SPIO nanoparticle ferrofluid, functionalized with the red-autofluorescing doxorubicin and the green-fluorescent dye fluorescein isothiocyanate as a reporter, enables tracking the intracellular nanoparticle transport and drug release...
October 29, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27816743/in-situ-tracking-the-reversible-spinel-rocksalt-structural-transformation-between-mn3o4-and-mno
#13
Xingyuan San, Bo Zhang, Jing Wang, Bo Wu, Xiuliang Ma
Electron beam irradiation is well known to induce damage in materials. The structural transformation involved in the damage is usually believed to be an irreversible solid state chemical reaction. Here we use in situ transmission electron microscopy (TEM) combined electron-energy loss spectroscopy (EELS) technique in an aberration-corrected TEM to track the structural transformation in spinel Mn3O4 induced by electron beam irradiation. It is clarified that spinel Mn3O4 is transformed to rocksalt structured MnO by irradiation and the reversed recovering transition from rocksalt MnO to spinel Mn3O4 can occur by aging in the gentle electron beam circumstance...
October 27, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27923156/improvement-of-effective-solid-angle-using-virtual-pivot-holder-and-large-eds-detector
#14
Shogo Koshiya, Koji Kimoto
This paper describes the effective solid angle improvement achieved using a large-area silicon drift detector together with a virtual-pivot double-tilt specimen holder. The virtual-pivot mechanism enables various designs of specimen-retaining and can reduce the shadowing effect. Energy-dispersive X-ray spectra were measured and converted into effective solid angles using different types of specimen holders and specimens. The investigated shadowing-free mechanical system yielded effective solid angles approaching the nominal solid angle of 0...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27918893/spectrum-image-analysis-tool-a-flexible-matlab-solution-to-analyze-eel-and-cl-spectrum-images
#15
Franz-Philipp Schmidt, Ferdinand Hofer, Joachim R Krenn
Spectrum imaging techniques, gaining simultaneously structural (image) and spectroscopic data, require appropriate and careful processing to extract information of the dataset. In this article we introduce a MATLAB based software that uses three dimensional data (EEL/CL spectrum image in dm3 format (Gatan Inc.'s DigitalMicrograph(®))) as input. A graphical user interface enables a fast and easy mapping of spectral dependent images and position dependent spectra. First, data processing such as background subtraction, deconvolution and denoising, second, multiple display options including an EEL/CL moviemaker and, third, the applicability on a large amount of data sets with a small work load makes this program an interesting tool to visualize otherwise hidden details...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27912140/characterization-of-the-cleaved-edge-cross-section-of-the-heterostructures-with-gamnas-layer-by-the-confocal-micro-raman-spectroscopy
#16
A V Kudrin, S M Plankina, O V Vikhrova, A V Nezhdanov, A I Mashin, Yu N Drozdov, A V Shvetsov
Confocal micro-Raman spectroscopy was used to measure cross-section linescans of the cleaved edge of heterostructures involving a GaMnAs layer. The investigations revealed a shift of the TO mode in the compressed GaMnAs layer to high frequencies relative to the TO GaAs mode in the substrate and to low frequencies in the tensile GaMnAs layers. These results are in agreement with the different manifestations of the anomalous Hall effect in the GaMnAs layers, with either compressive or tensile strains. It is shown that Raman spectroscopy is an appropriate method for the investigation of cross-sectional semiconductor heterostructures whose total thickness is comparable to the size of the analyzing laser spot...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27912139/repair-of-calvarial-bone-defects-in-mice-using-electrospun-polystyrene-scaffolds-combined-with-%C3%AE-tcp-or-gold-nanoparticles
#17
Lisa Terranova, Diana Maria Dragusin, Romain Mallet, Eugeniu Vasile, Izabela-Cristina Stancu, Catherine Behets, Daniel Chappard
Non-biodegradable porous polystyrene (PS) scaffolds, composed of microfibers, have been prepared by electrospinning for the reconstruction of large bone defects. PS microfibers were prepared by incorporating β-TCP grains inside the polymer or grafting gold nanoparticles surface functionalized with mercaptosuccinic acid. Cytocompatibility of the three types of scaffolds (PS, β-TCP-PS and Au-PS) was studied by seeding human mesenchymal stem cells. Biocompatibility was evaluated by implanting β-TCP-PS and Au-PS scaffolds into a critical size (4mm) calvarial defect in mice...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27883991/a-comparative-morphological-study-of-the-epiandrous-apparatus-in-mygalomorph-spiders-araneae-mygalomorphae
#18
N Ferretti, G Pompozzi, S Copperi, A Wehitt, E Galíndez, A González, F Pérez-Miles
Many adult male spiders have silk glands which are not associated with the spinnerets. They occur on the anterior margin of the genital furrow and are used during the building of the sperm web and sperm droplet induction. These epiandrous glands exit the body through ducts which lead to specialized spigots. In the taxon Mygalomorphae, the presence of epiandrous spigots is just reported for a couple of species but their morphology has not been investigated. In this paper we provide a detailed study of the ultrastructure morphology of eighteen species belonging to eight families using light and scanning electron microscopy (SEM)...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27883990/force-distance-curves-on-lubricant-films-an-approach-to-the-characterization-of-the-shape-of-the-afm-tip
#19
Brunero Cappella
Force-distance curves have been acquired with an atomic force microscope on perfluorpolyether films. It is shown that curves acquired on films of different thickness, at different rates and with different dwell times can be overlapped by rescaling the time or distance axis. When the time or distance axis is rescaled, the force depends only on the surface tension of perfluorpolyether and on geometrical properties of the tip (aperture and perimeter of the tip at a given distance from the apex). Hence, curves acquired with the same tip overlap...
February 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27883989/recombinant-anthrax-protective-antigen-observation-of-aggregation-phenomena-by-tem-reveals-specific-effects-of-sterols
#20
J Robin Harris, Andrei Soliakov, Allan Watkinson, Jeremy H Lakey
Negatively stained transmission electron microscope images are presented that depict the aggregation of recombinant anthrax protective antigen (rPA83 monomer and the PA63 prepore oligomer) under varying in vitro biochemical conditions. Heat treatment (50°C) of rPA83 produced clumped fibrils, but following heating the PA63 prepore formed disordered aggregates. Freeze-thaw treatment of the PA63 prepore generated linear flexuous aggregates of the heptameric oligomers. Aqueous suspensions of cholesterol microcrystals were shown to bind small rPA83 aggregates at the edges of the planar bilayers...
February 2017: Micron: the International Research and Review Journal for Microscopy
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