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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/29190503/the-interaction-between-human-blood-neutrophil-granulocytes-and-quantum-dots
#1
Svetlana N Pleskova, Elza R Mikheeva, Ekaterina E Gornostaeva
For biomedical applications, it is important to know, which kinds of blood cells can capture quantum dots (QDs). The maximum accumulation of QDs was found for the monocyte fraction of leukocytes, the minimum binding of QDs was observed for lymphocytes. It was found that CdSe/ZnS-MPA QDs are actively absorbed by the cells and have more expressed toxicity. The classical mechanism of the phagocytosis of QDs was revealed for neutrophils, when the QDs are located in phagolysosomes. The capture of QDs by neutrophil granulocytes has resulted in a destruction of certain types of QDs...
November 23, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29179010/method-for-estimating-modulation-transfer-function-from-sample-images
#2
Rino Saiga, Akihisa Takeuchi, Kentaro Uesugi, Yasuko Terada, Yoshio Suzuki, Ryuta Mizutani
The modulation transfer function (MTF) represents the frequency domain response of imaging modalities. Here, we report a method for estimating the MTF from sample images. Test images were generated from a number of images, including those taken with an electron microscope and with an observation satellite. These original images were convolved with point spread functions (PSFs) including those of circular apertures. The resultant test images were subjected to a Fourier transformation. The logarithm of the squared norm of the Fourier transform was plotted against the squared distance from the origin...
November 22, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29179009/osteoblastic-cell-behaviour-on-modified-titanium-surfaces
#3
Magdalena Lukaszewska-Kuska, Przemysław Wirstlein, Radomir Majchrowski, Barbara Dorocka-Bobkowska
INTRODUCTION: The surfaces of endoosseous dental implants have been subjected to numerous modifications in order to create a surface which can provide rapid bone healing and fast implant loading. Each modification has involved changes to the chemical composition and topography of the surfaces which have resulted in various biological reactions to the implanted material. AIM: The aim of this study was to evaluate the surface topography and chemistry of various modified titanium surfaces: (1) machined surface (MA), (2) alumina-blasted (Al2O3), (3) alumina-blasted and acid-etched (Al2O3 DE), (4) hydroxyapatite/tricalcium phosphate grit-blasted (HA/TCP) and (5) hydroxyapatite/tricalcium phosphate grit-blasted and acid-etched (HA/TCP DE) and to analyse the effects of surface roughness, and chemical composition on human osteoblast vitality, differentiation, morphology and orientation...
November 22, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29179007/electron-microscopy-observations-on-testis-and-spermatozoa-of-leptodactylus-chaquensis-anura-leptodactylidae
#4
Lucrecia Iruzubieta Villagra, Inés Ramos, Susana Cisint, Claudia A Crespo, Silvia N Fernández
Scanning and transmission electron microscopy were used to investigate the fine structure of the testis and spermatozoa of toad Leptodactylus chaquensis. Our observations indicate that germinal compartment contains Sertoli (SC) and germ cells. The tight junctions and desmosomes among SC indicate the existence of an hematotesticular barrier in charge of maintaining the differences in the composition of the germinal and interstitial compartments. During spermatogenesis, SC acts as a structural support for germ cells...
November 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29175448/in-situ-investigation-of-bismuth-nanoparticles-formation-by-transmission-electron-microscope
#5
Liming Liu, Honghang Wang, Zichuan Yi, Quanrong Deng, Zhidong Lin, Xiaowen Zhang
Bismuth (Bi) nanoparticles are prepared by using NaBi(MoO4)2 nanosheets in the beam of electrons emitted by transmission electron microscope. The formation and growth of Bi nanoparticles are investigated in situ. The sizes of Bi nanoparticles are confined within the range of 6-10nm by controlling irradiation time. It is also observed that once the diameter of nanoparticles is larger than 10nm, the Bi particles are stable as a result of the immobility of large nanoparticles. In addition, some nanoparticles on the edges form nanorods, which are explained as the result of a coalescence process, if the irradiation period is longer than 10min...
November 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29179008/oblique-illumination-in-microscopy-a-quantitative-evaluation
#6
Carlos Sanchez, Gabriel Cristóbal, Gloria Bueno, Saul Blanco, Maria Borrego-Ramos, Adriana Olenici, Anibal Pedraza, Jesus Ruiz-Santaquiteria
Many biological objects are barely distinguished with the brightfield microscope because they appear transparent, translucent and colourless. One simple way to make such specimens visible without compromising contrast and resolution is by controlling the amount and the directionality of the illumination light. Oblique illumination is an old technique described by many scientists and microscopists that however has been largely neglected in favour of other alternative methods. Oblique lighting (OL) is created by illuminating the sample by only a portion of the light coming from the condenser...
November 16, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29169143/energy-dissipation-mapping-of-cancer-cells
#7
Diganta Dutta, Xavier-Lewis Palmer, Jinhyun Kim, Shizhi Qian, Michael Stacey
The purpose of this study is to map the energy dissipation of Jurkat cells using a single 60 nanosecond pulse electric field (NsPEF), primarily through atomic force microscopy (AFM). The phase shift is generated by the sample elements that do not have a heterogeneous surface. Monitoring and manipulating the phase shift is a powerful way for determining the dissipated energy and plotting the topography. The dissipated energy is a relative value, so the silica wafer and cover slip are given a set reference while the transmission of energy between the tip of the cantilever and cell surfaces is measured...
November 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29145009/sperm-morphology-of-predatory-pirate-bugs-amphiareus-constrictus-and-blaptostethus-pallescens-heteroptera-anthocoridae-with-phylogenetic-inferences
#8
Aline Beatriz Reis Santos, José Lino-Neto
The sperm morphology of two predatory bugs Amphiareus constrictus and Blaptostethus pallescens, representing the tribes Dufouriellini and Blasptostethini, respectively, was described using light and transmission electron microscopy. The spermatozoa of Amphiareus constrictus and Blaptostethus pallescens are fine and long, each measuring 216.6μm and 181.0μm in length, of which 37.0μm and 11.6μm, respectively, comprise the nuclei. When stained with DAPI (for DNA), the posterior half of the nucleus in B. pallescens exhibited low fluorescence, while in A...
November 11, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29145008/morphological-changes-in-erythrocytes-of-people-with-type-2-diabetes-mellitus-evaluated-with-atomic-force-microscopy-a-brief-review
#9
REVIEW
Alejandra Loyola-Leyva, Juan Pablo Loyola-Rodríguez, Marco Atzori, Francisco Javier González
Prevalence of type 2 diabetes mellitus (T2DM) has been increasing worldwide. Cardiovascular diseases are one of the main causes of death among people with T2DM. Morphological changes in erythrocytes have been associated with higher risk of cardiovascular diseases. Atomic force microscopy (AFM) is a new technique that allows non-invasive imaging of cells and the evaluation of changes in mechanical properties. AIM: To evaluate by AFM the erythrocytes morphological changes of people with T2DM METHODS: Search was conducted from in PubMed, ScienceDirect, Scielo, and Lilacs...
November 10, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29132029/reconstruction-of-atomic-force-microscopy-image-using-compressed-sensing
#10
Guoqiang Han, Bo Lin, Yuling Lin
Atomic Force Microscopy (AFM) is one of the most popular and advanced tools for ultra high-resolution imaging and nanomanipulation of nano-scale matter. But AFM imaging typically takes a long time. High-speed and high-precision AFM measurement has attracted wide attention in recent several years. In traditional AFM, simple reduction in the number of measurement points may lose essential sample topography information. To resolve such problems, an AFM image reconstruction method based on Compressed Sensing (CS) theory is applied to reduce image acquisition time without cutting down the image quality...
November 10, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29125996/characterization-of-wear-debris-released-from-alumina-on-alumina-hip-prostheses-analysis-of-retrieved-femoral-heads-and-peri-prosthetic-tissues
#11
Louis Rony, Pierre de Sainte Hermine, Vincent Steiger, Romain Mallet, Laurent Hubert, Daniel Chappard
We analyzed by SEM three alumina-on-alumina femoral heads obtained from three patients who underwent revision for an aseptic loosening of the acetabular component. In parallel, the peri-prosthetic tissues were analyzed histologically in search of wear debris coming from the ceramic. Stripe wears, abrasive streaks, craters, and areas with extensive biomaterial removal were evidenced on the three femoral heads by SEM. In the altered area, the structure of the ceramic composed of minute polyhedric grains packed together was evidenced...
November 4, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29112916/morphology-and-ultrastructure-of-dufour-s-and-venom-glands-in-the-ant-camponotus-japonicus-mayr-hymenoptera-formicidae
#12
Ying Zhou, Changyou Li, Johan Billen, Hong He
The Dufour's and venom glands are two important exocrine glands in ants. In this study, the morphology and fine structure of these two glands are described in the ant Camponotus japonicus Mayr. The Dufour glands have a characteristic bilobed shape and show a difference in size and color between the female castes (minor and major workers, alate and dealate queens). The external surface of Dufour's gland shows different features among the female castes. It appears more hypertrophied in major workers than in the other castes, indicating for a more pronounced function...
October 31, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29102797/morphology-and-ultrastructure-of-the-mandibular-gland-in-the-ant-brachyponera-sennaarensis-hymenoptera-formicidae
#13
Johan Billen, Mohammed Al-Khalifa
The 'samsum ant' Brachyponera sennaarensis is an invasive species in Saudi Arabia, where it forms a serious threat because of its painful sting. As part of a morphological survey of the exocrine system of this species, we studied the mandibular gland of males, queens and workers of this species. The gland of males is similar to the common anatomical appearance the mandibular gland has in ants in general, but is considerably different in queens and workers. In both female castes, the secretory cells are grouped in one single cluster, that is surrounded by a thick sheath of connective tissue...
October 31, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29101880/in-situ-observation-of-phase-transformation-in-iron-carbide-nanocrystals
#14
Le Thanh Cuong, Nguyen Duc Dung, Ta Quoc Tuan, Nguyen Thi Khoi, Pham Thanh Huy, Ngo Ngoc Ha
This paper reports on the in situ observation of phase transformation in an iron carbide nanocrystal encapsulated in a graphitic shell by means of high resolution transmission electron microscopy (HR-TEM). A Fe7C3 nanocrystal in orthorhombic (o-Fe7C3) structure with carbon graphitic cover is captured at the initial time of the experiment. Under the projection of a high-energy electron beam (200kV), the graphitic carbon layer evaporates gradually and structural changes in orthorhombic (o-Fe7C3) crystal manifests simultaneously...
October 31, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29112917/influence-of-minor-combined-addition-of-cr-and-pr-on-microstructure-mechanical-properties-and-corrosion-behaviors-of-an-ultrahigh-strength-al-zn-mg-cu-zr-alloy
#15
Ming Wang, Lanping Huang, Kanghua Chen, Wensheng Liu
This work focuses on controlling grain boundary structure in an ultra-high strength Al-8.6Zn-2.5Mg-2.2Cu-0.16Zr (wt.%) alloy by the combined addition of trace Cr (0.1wt.%) and Pr (0.14wt.%), and evaluating mechanical properties and localized corrosion behaviors of the alloy in the peak aged condition. The introduction of trace Cr and Pr leads to the formation of nanoscale Cr, Pr-containing Al3Zr and Zr-containing PrCr2Al20 dispersoids which can obviously inhibit the recrystallization and sub-grain growth of the super-high strength Al-Zn-Mg-Cu alloys, and retain the deformation-recovery microstructure dominated by low-angle grain boundaries...
October 26, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29080547/detection-of-secondary-and-backscattered-electrons-for-3d-imaging-with-multi-detector-method-in-vp-esem
#16
Witold Slówko, Artur Wiatrowski, Michał Krysztof
The paper considers some major problems of adapting the multi-detector method for three-dimensional (3D) imaging of wet bio-medical samples in Variable Pressure/Environmental Scanning Electron Microscope (VP/ESEM). The described method pertains to "single-view techniques", which to create the 3D surface model utilise a sequence of 2D SEM images captured from a single view point (along the electron beam axis) but illuminated from four directions. The basis of the method and requirements resulting from them are given for the detector systems of secondary (SE) and backscattered electrons (BSE), as well as designs of the systems which could work in variable conditions...
October 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29190502/nacl-changes-stem-morphology-anatomy-and-phloem-structure-in-lucerne-medicago-sativa-cv-gab%C3%A3-s-comparison-of-upper-and-lower-internodes
#17
Riheb Ben Nja, Bruno Merceron, Mireille Faucher, Pierrette Fleurat-Lessard, Emile Béré
In M. sativa cv. Gabès plants treated with 150mM NaCl, the height of the stem is decreased and the internode number, length and diameter are reduced. This depressive effect on growth, but also on photosynthetic activity and water balance, is accompanied by structural changes. In the upper internodes, NaCl treatment increases cambium development, so that the vascular ring is initiated earlier than in controls. In the lower internodes, the number of lignified phloem fibers is increased by NaCl, and their wall thickness is augmented, compared to controls; in the phloem complex, the nacreous layer is enlarged, the number of internal wall ingrowths is increased, but companion cells are damaged...
October 18, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29049927/electron-tomography-of-whole-cultured-cells-using-novel-transmission-electron-imaging-technique
#18
Taiga Okumura, Minami Shoji, Akiko Hisada, Yusuke Ominami, Sukehiro Ito, Tatsuo Ushiki, Masato Nakajima, Takashi Ohshima
Since a three-dimensional (3D) cellular ultrastructure is significant for biological functions, it has been investigated using various electron microscopic techniques. Although transmission electron microscopy (TEM)-based techniques are traditionally used, cells must be embedded in resin and sliced into ultrathin sections in sample preparation processes. Block-face observation using a scanning electron microscope (SEM) has also been recently applied to 3D observation of cellular components, but this is a destructive inspection and does not allow re-examination...
October 16, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29049928/developmental-changes-in-haemocyte-morphology-in-response-to-staphylococcus-aureus-and-latex-beads-in-the-beetle-tenebrio-molitor-l
#19
Arkadiusz Urbański, Zbigniew Adamski, Grzegorz Rosiński
The evolutionary success of insects is undoubtedly related to a well-functioning immune system. This is especially apparent during insect development by the adaptation of individuals to the changing risk of infection. In addition, current studies show that the insect immune system is characterized by some specificity in response to natural pathogens (for example, bacteria, viruses or fungi) and artificial challengers (for example, latex beads or nylon filaments). However, developmental changes and the specificity of immune system reactions simultaneously have not been analysed...
October 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29073496/experimental-study-and-numerical-simulation-on-the-structural-and-mechanical-properties-of-typha-leaves-through-multimodal-microscopy-approaches
#20
Jingjing Liu, Zhihui Zhang, Zhenglei Yu, Yunhong Liang, Xiujuan Li, Luquan Ren
The Typha leaf, with special multi-level structure, low density and excellent mechanical properties, is an ideal bionic prototype utilized for lightweight design. In order to further study the relationship between the structure and mechanical properties, the three-dimensional macroscopic morphology of Typha leaves was characterized by micro computed tomography (Micro-CT) and its internal microstructure was observed by scanning electron microscopy (SEM). The combination of experimental and computational research was carried out in this paper, to reveal and verify the effect of multi-level structure on the mechanical properties...
October 12, 2017: Micron: the International Research and Review Journal for Microscopy
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