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Micron: the International Research and Review Journal for Microscopy

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https://www.readbyqxmd.com/read/28514702/nanoscale-morphological-analysis-of-soft-matter-aggregates-with-fractal-dimension-ranging-from-1-to-3
#1
REVIEW
Francesco Valle, Marco Brucale, Stefano Chiodini, Eva Bystrenova, Cristiano Albonetti
While the widespread emergence of nanoscience and nanotechnology can be dated back to the early eighties, the last decade has witnessed a true coming of age of this research field, with novel nanomaterials constantly finding their way into marketed products. The performance of nanomaterials being dominated by their nanoscale morphology, their quantitative characterization with respect to a number of properties is often crucial. In this context, those imaging techniques able to resolve nanometer scale details are clearly key players...
May 4, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28494437/nanosecond-pulsed-electric-field-induced-changes-in-cell-surface-charge-density
#2
Diganta Dutta, Xavier-Lewis Palmer, Anthony Asmar, Michael Stacey, Shizhi Qian
This study reports that the surface charge density changes in Jurkat cells with the application of single 60 nanosecond pulse electric fields, using atomic force microscopy. Using an atomic force microscope tip and Jurkat cells on silica in a 0.01M KCl ionic concentration, we were able to measure the interfacial forces, while also predicting surface charge densities of both Jurkat cell and silica surfaces. The most important finding is that the pulsing conditions varyingly reduced the cells' surface charge density...
May 2, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28500930/micro-computed-tomography-scanning-electron-microscopy-and-energy-x-ray-spectroscopy-studies-of-facet-joint-degeneration-a-comparison-to-clinical-imaging
#3
Felix Goehre, Christopher Ludtka, Melanie Hamperl, Andrea Friedmann, Anja Straube, Thomas Mendel, Andreas Heilmann, Hans Jörg Meisel, Stefan Schwan
Segmental degeneration in the human lumbar spine affects both the intervertebral discs and facet joints. Facet joint degeneration not only affects the cartilage surface, but also alters the cellular properties of the cartilage tissue and the structure of the subchondral bone. The primary focus of this study is the investigation of these microstructural changes that are caused by facet joint degeneration. Microstructural analyses of degenerated facet joint samples, obtained from patients following operative lumbar interbody fusion, have not previously been extensively investigated...
May 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28482176/exosomes-ultrastructural-evidence-in-epithelial-cells-of-malpighian-tubules
#4
Anita Giglio, Ida Daniela Perrotta, Pietro Brandmayr
Exosomes in Malpighian tubules of Calathus fuscipes (Coleoptera: Carabidae) were investigated under transmission electron microscopy. Ultrastructural analyses showed a wide number of multivesicular bodies localized in the apical portion of epithelial cells. Each multivesicular body encloses from 15 to 80 intraluminal vesicles (about 50nm in diameter), originating through inward budding of late endosomes that package molecules into luminal membrane-bound structures. Subsequently they are released as exosomes through exocytosis of multivesicular bodies into the extracellular space after fusion with plasma membrane...
April 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28527398/quantitative-comparison-between-real-space-and-bloch-wave-methods-in-image-simulation
#5
Yi Yang, Qibin Yang, Jianyu Huang, Canying Cai, Jianguo Lin
Both Bloch wave (BW) and real space multislice (RS-MS) methods are based on the Schrödinger equation. The BW method is considered to be the most accurate and popular simulation method used in various simulations, and it has solved many crystallographic problems. In this paper, we verified that the aforementioned two methods are similar to each other with minor difference. First, the RS-MS method is implemented in the real space, while the BW method is carried out in the reciprocal space. Furthermore, by quantitatively calculating exit wavefunctions of four crystals, i...
April 27, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28486138/the-effects-of-titanium-dioxide-nanoparticles-on-ultrastructure-of-zebrafish-testis-danio-rerio
#6
Tuğba Kotil, Cansu Akbulut, Nazan Deniz Yön
INTRODUCTION: Nanotechnology investigates materials at nanoscale level (0.1-100nm in diameter). There are many commercially nanoproducts such as silver, silicon, titanium, zinc, and gold. They are used in a variety of applications and released to the environment. Titanium dioxide (TiO2) is one of the most commonly used nanoparticles (NP). In this study, the ultrastructural effects of TiO2-NP on zebrafish testis tissue were evaluated. MATERIAL AND METHOD: Zebrafish were divided into four groups (N=60) as one control and 3 experimental groups (1mg/L, 2mg/L and 4mg/L TiO2)...
April 26, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28477556/giant-vesicles-gv-in-colloidal-system-under-the-optical-polarization-microscope-opm
#7
Khalisanni Khalid, Muhammad Azri Mohd Noh, M Niyaz Khan, Ruzaina Ishak, Esther Penney, Zaira Zaman Chowdhury, Mohammad Hafiz Hamzah, Maizatulnisa Othman
This paper discusses the unprecedented microscopic findings of micellar growth in colloidal system (CS) of catalyzed piperidinolysis of ionized phenyl salicylate (PS(-)). The giant vesicles (GV) was observed under the optical polarization microscope (OPM) at [NaX]=0.1M where X=3-isopropC6H4O(-). The conditions were rationalized from pseudo-first-order rate constant, kobs of PS(-) of micellar phase at 31.1×10(-3)s(-1) reported in previous publication. The overall diameter of GV (57.6μm) in CS (CTABr/NaX/H2O)-catalyzed piperidinolysis (where X=3-isopropC6H4O) of ionized phenyl salicylate were found as giant unilamellar vesicles (GUV) and giant multilamellar vesicles (GMV)...
April 26, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28463750/visualization-of-macro-immune-complexes-in-the-antiphospholipid-syndrome-by-multi-modal-microscopy-imaging
#8
Douglas J Taatjes, Nicole Bouffard, Michele von Turkovich, Anthony S Quinn, Xiao-Xuan Wu, Ljiljana V Vasovic, Jacob H Rand
The antiphospholipid syndrome (APS) is an autoimmune thrombotic condition that is marked by autoantibodies against phospholipid-binding proteins. The mechanism(s) of thrombogenesis has (have) resisted elucidation since its description over thirty years ago. Nevertheless, a defining aspect of the disorder is positivity for clinical laboratory tests that confirm antibody binding to anionic phospholipids. It is remarkable that, to our knowledge, the binding of proteins from plasmas of APS patients to phospholipid has not been previously imaged...
April 24, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28448830/biogenesis-of-secondary-mycogenic-minerals-related-to-wall-paintings-deterioration-process
#9
Nikola Unković, Suzana Erić, Kristina Šarić, Miloš Stupar, Željko Savković, Slaviša Stanković, Olja Stanojević, Ivica Dimkić, Jelena Vukojević, Milica Ljaljević Grbić
Present study addresses potential of fungal strains, isolated from deteriorated mural paintings and surrounding air environment of the Church of the Holy Ascension in Veliki Krčimir (Serbia), to precipitate mycogenic minerals, when cultivated on agarized B4 medium. Utilizing culture-based isolation methods, 38 filamentous fungi were obtained in total, 23 from mural paintings and 15 from air, respectively, mainly ascomycetes, while Bjerkandera adusta and Thanatephorus cucumeris were only basidiomycetes. A total of 31 of 38 fungal isolates, more than 80%, were able to form minerals of different morphologies and variable size, determined via SEM-EDS and XRPD, to be either calcite or calcite and weddellite association...
April 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28448831/computer-simulations-analysis-for-determining-the-polarity-of-charge-generated-by-high-energy-electron-irradiation-of-a-thin-film
#10
Marek Malac, Simon Hettler, Misa Hayashida, Masahiro Kawasaki, Yuji Konyuba, Yoshi Okura, Hirofumi Iijima, Isamu Ishikawa, Marco Beleggia
Detailed simulations are necessary to correctly interpret the charge polarity of electron beam irradiated thin film patch. Relying on systematic simulations we provide guidelines and movies to interpret experimentally the polarity of the charged area, to be understood as the sign of the electrostatic potential developed under the beam with reference to a ground electrode. We discuss the two methods most frequently used to assess charge polarity: Fresnel imaging of the irradiated area and Thon rings analysis...
April 3, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28371728/pore-size-distributions-convolution-for-microtomographic-images-applied-to-shark-bay-s-oolite
#11
Paulo J Dos Reis, Rodrigo Nagata, Carlos R Appoloni
In the study of pore size distributions within microtomographic images, there is no consensus in how to group pore size distributions obtained at different spatial resolutions in a single distribution, which represents the properties of the sample in a better way. This work presents a proposition that allows to group pore size distributions obtained by microtomography images in different spatial resolutions. A Shark's Bay oolite was imaged by X-ray microtomography at 4.4μm and 1.1μm spatial resolutions. The pore size distribution and average porosity were determined for each resolution...
March 25, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28342371/contrast-enhancement-with-uranyl-acetate-allows-quantitative-analysis-of-the-articular-cartilage-by-microct-application-to-mandibular-condyles-in-the-btx-rat-model-of-disuse
#12
Jean-Daniel Kün-Darbois, Florence Manero, Louis Rony, Daniel Chappard
Microcomputed tomography (microCT) is well adapted to quantitative analysis of calcified tissues but soft tissues (such as cartilage) are radiolucent and need a contrast enhancement procedure for microCT. We developed a "staining" method allowing microCT imaging of articular cartilage using uranyl acetate (UA). The method was used to see whether adult rats with a botulinum toxin (BTX) injection in masticatory muscles present a change at the condylar cartilage of the mandible in association with a localized trabecular bone loss...
March 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28343096/sd-sem-sparse-dense-correspondence-for-3d-reconstruction-of-microscopic-samples
#13
Ahmadreza Baghaie, Ahmad P Tafti, Heather A Owen, Roshan M D'Souza, Zeyun Yu
Scanning electron microscopy (SEM) imaging has been a principal component of many studies in biomedical, mechanical, and materials sciences since its emergence. Despite the high resolution of captured images, they remain two-dimensional (2D). In this work, a novel framework using sparse-dense correspondence is introduced and investigated for 3D reconstruction of stereo SEM images. SEM micrographs from microscopic samples are captured by tilting the specimen stage by a known angle. The pair of SEM micrographs is then rectified using sparse scale invariant feature transform (SIFT) features/descriptors and a contrario RANSAC for matching outlier removal to ensure a gross horizontal displacement between corresponding points...
March 20, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28364625/study-of-temperature-effects-on-the-electrical-behavior-of-polypropylene-clay-nanocomposites-submitted-to-electron-beam-irradiation-in-a-sem
#14
Latifa Ben Ammar, Slim Fakhfakh, Omar Jbara, Sebastien Rondot, Aomar Hadjadj
Charge transport and electron emission properties in polypropylene and its nanocomposites filled with nanoclay particles submitted to an electron irradiation, in a Scanning Electron Microscope (SEM), are investigated using induced displacement and leakage currents. The measurements have been performed at various temperatures ranging from 20°C to 75°C at a primary beam energy of 20keV and a primary beam current of 1nA with the aim to highlight the effect of temperature and nanoclay content on these properties...
March 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28359958/atom-probe-study-of-b2-order-and-a2-disorder-of-the-feco-matrix-in-an-fe-co-mo-alloy
#15
C Turk, H Leitner, I Schemmel, H Clemens, S Primig
The physical and mechanical properties of intermetallic alloys can be tailored by controlling the degree of order of the solid solution by means of heat treatments. FeCo alloys with an appropriate composition exhibit an A2-disorder↔B2-order transition during continuous cooling from the disordered bcc region. The study of atomic order in intermetallic alloys by diffraction and its influence on the material properties is well established, however, investigating magnetic FeCo-based alloys by conventional methods such as X-ray diffraction is quite challenging...
March 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28359957/structure-and-components-of-the-globular-and-filamentous-viroplasms-induced-by-rice-black-streaked-dwarf-virus
#16
Li Xie, Ming-Fang Lv, Xi-Jiao Song, Jian Yang, Jing Li, Zong-Tao Sun, Ying-Yi Zhang, Jian-Ping Chen, Heng-Mu Zhang
Viroplasms of members of the family Reoviridae are considered to be viral factories for genome replication and virion assembly. Globular and filamentous phenotypes have different components and probably have different functions. We used transmission electron microscopy and electron tomography to examine the structure and components of the two viroplasm phenotypes induced by Rice black-streaked dwarf virus (RBSDV). Immuno-gold labeling was used to localize each of the 13 RBSDV encoded proteins as well as double-stranded RNA, host cytoskeleton actin-11 and α-tubulin...
March 16, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28359956/ab-initio-study-of-the-energy-loss-near-sulfur-k-and-l2-3-edges-of-layered-ms2-m-ta-nb-and-v-in-trigonal-prismatic-and-octahedral-structures
#17
Mehrdad Dadsetani, Tahereh Nouri, Hajar Nejatipour
This study set out to calculate the full-potential linearized augmented plane wave (FPLAPW)-based energy loss near sulfur K and L2,3 edge structures of group V layered transition metal disulfides MS2 (M=Ta, Nb, and V) in octahedral (1T) as well as trigonal prismatic (2H) structures. The calculations showed that, consistent with other calculations, all the studied materials were metallic due to the partially filled d bands in their configurations. Furthermore, the calculated ELNES spectra revealed a good agreement with the available experimental XANES analogues...
March 14, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28342370/fabrication-and-tribological-properties-of-nanogrids-on-cvd-grown-graphene
#18
Yan Jiang, Yu Sun, Juan Song
We have used atomic force microscopy (AFM) combined with an external sourcemeter to make patterns on CVD-grown graphene, and regular nanogrids have been fabricated on graphene by AFM tip-induced local oxidation. The friction and adhesion properties of graphene with the nanogrids have been characterized by the lateral mode of AFM and the results show that the friction force on the borders of nanogrid is higher than that on the normal area of graphene, yet the adhesion force on the nanogrids decrease slightly due to the change of surface roughness...
March 12, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28371643/self-interference-of-split-holz-line-sis-holz-for-z-dependent-atomic-displacement-measurement-theoretical-discussion
#19
Mana Norouzpour, Ramtin Rakhsha, Rodney Herring
A characteristic of the majority of semiconductors is the presence of lattice strain varying with the nanometer scale. Strain originates from the lattice mismatch between layers of different composition deposited during epitaxial growth. Strain can increase the mobility of the charge carriers by the band gap reduction. So, measuring atomic displacement inside crystals is an important field of interest in semiconductor industry. Among all available transmission electron microscopy techniques offering nano-scale resolution measurements, convergent beam electron diffraction (CBED) patterns show the highest sensitivity to the atomic displacement...
March 10, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28346884/automatic-measurement-of-melanoma-depth-of-invasion-in-skin-histopathological-images
#20
Hongming Xu, Richard Berendt, Naresh Jha, Mrinal Mandal
Measurement of melanoma depth of invasion (DoI) in skin tissues is of great significance in grading the severity of skin disease and planning patient's treatment. However, accurate and automatic measurement of melanocytic tumor depth is a challenging problem mainly due to the difficulty of skin granular identification and melanoma detection. In this paper, we propose a technique for measuring melanoma DoI in microscopic images digitized from MART1 (i.e., meleanoma-associated antigen recognized by T cells) stained skin histopathological sections...
March 10, 2017: Micron: the International Research and Review Journal for Microscopy
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