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Biological & Pharmaceutical Bulletin

Takayuki Ishii, Kana Miyauchi, Yoshiharu Nitta, Kazuhiro Kaneko, Takuro Maruyama, Takeshi Sato
Upon differentiation of cells, remarkable changes in the structures of glycans linked to lipids on cell surface have been observed. Lactosylceramide (Lac-Cer) serves as a common precursor for a series of glycosphingolipids with diverse structures. In the present study, we examined the underlying mechanism for the biosynthesis of Lac-Cer upon differentiation of 3T3-L1 mouse preadipocytes to adipocytes. TLC analysis showed that the amounts of Lac-Cer decrease in 3T3-L1 adipocytes compared to 3T3-L1 preadipocytes...
July 7, 2018: Biological & Pharmaceutical Bulletin
Yue-Hua Jiang, Ling-Yu Jiang, Sai Wu, Wen-Jun Jiang, Lifang Xie, Wei Li, Chuan-Hua Yang
Tribulus terrestris L (Zygophyllaceae) (TT) is usually used as a cardiotonic, diuretic, and aphrodisiac, as well as for herbal post-stroke rehabilitation in traditional Chinese medicine. However, little is known about the renoprotective effects of TT on obesity-related glomerulopathy (ORG). In this study, 340 monomeric compounds were identified from TT extracts obtain with ethyl acetate combined with 50% methanol. In vitro, IC50 of TT was 912.01 mg/L, and the appropriate concentration of TT against ox-LDL induced human renal glomerular endothelial cells (HRGECs) was 4 mg/L...
July 6, 2018: Biological & Pharmaceutical Bulletin
Dalin Sun, Weihang Dong, Baofang Jin, Guanghui Chen, Bin Cai, Weimin Deng, Yugui Cui, Yihan Jin
The present study aims to investigate the roles of StAR in Yangjing Capsule (YC) induced anti-apoptotic effects on Leydig cells and the related mechanism. Leydig tumor cells (MLTC-1) were cultured and treated with YC, and immunofluorescence assay was performed to examine the expression of StAR; furthermore, luciferase reporter assay was conducted to evaluate the impact of YC on StAR promoter; next, MLTC-1 cells were treated with StAR siRNA, and flow cytometry was carried out to examine the effect of StAR siRNA on the apoptosis of the cells; furthermore, qRT-PCR and western blot methods was used to determine the expression of StAR and apoptosis related molecules Bcl-2, Bax and Caspase-3 on both mRNA and protein levels in different groups; finally the secretion of testosterone in different groups was examined by radioimmunoassay...
July 6, 2018: Biological & Pharmaceutical Bulletin
Takako Kato, Yoko Idota, Kazuyo Shiragami, Mariko Koike, Fumiya Nishibori, Miho Tomokane, Takahiro Seki, Keiko Itabashi, Keiko Hakoda, Hitoe Takahashi, Kentaro Yano, Tsutomu Kobayashi, Nobuyuki Obara, Takuo Ogihara
We conducted a prospective, randomized, double-blind, 3-group, 3-phase crossover study to evaluate the effect of calcium alginate (Ca-Alg) on the postprandial increase of blood glucose in 15 healthy adult subjects who were given udon noodles containing or not containing Ca-Alg (5% or 8%). The value of ΔCmax (difference between the maximum (Cmax ) and pre-feeding (C0 ) blood glucose levels) was significantly reduced in both Ca-Alg groups, and the area under the blood glucose level-time curve over 120 min (ΔAUC, with C0 as the baseline) was also significantly reduced...
July 4, 2018: Biological & Pharmaceutical Bulletin
Yoko Idota, Takako Kato, Kazuyo Shiragami, Mariko Koike, Akari Yokoyama, Hitoe Takahashi, Kentaro Yano, Takuo Ogihara
Calcium alginate (Ca-Alg) is known to suppress the postprandial increase of blood glucose, and therefore may be helpful for preventing lifestyle-related diseases such as diabetes. In this work, we examined the mechanism of this effect. As α-amylase activity and α-glucosidase activity are involved in the digestion of starch, we examined the in vitro inhibitory effect of Ca-Alg on these enzymes. Ca-Alg showed little inhibition of α-amylase, but markedly inhibited α-glucosidase activity. The direct binding affinity of glucose for Ca-Alg was low...
July 4, 2018: Biological & Pharmaceutical Bulletin
Wang Yan-Yan, Li Yan-Yan, Li Lin, Yang Dong-Li, Zhou Kun, Li Yu-Hong
The aim of the present study was to investigate the protective effects of Shenfu injection (SFI) against myocardial ischemia/reperfusion injury (MIRI) in model rats and to explore its mechanism of action. Sprague-Dawley (SD) rats were pretreated with SFI and L-NAME via tail vein injection and then rats were subjected to ischemia by occlusion of the left anterior descending coronary artery for 30min followed by reperfusion for 120min. Left ventricular function was evaluated by echocardiography. Hemodynamic was measured by the Millar pressure - volume system; serum creatine kinase (CK), lactate dehydrogenase (LDH) and serum troponin (TNNI3) levels were determined...
June 16, 2018: Biological & Pharmaceutical Bulletin
Lu Xu, Jieyi Cai, Aofei Tian, Kai Qian, Renan Qin, Shaoyun Qi, Xupeng Tan, Yuqin Qiu, Mengjuan Gong, Bin Han, Xuguang Hu
The intestinal barrier dysfunction is a critical pathological change in irritable bowel syndrome (IBS). The objective of this study was to evaluate the effect of Prim-O-glucosylcimifugin (POG) on intestinal barrier dysfunction and reveal possible molecular mechanisms. Caco-2 cell monolayers induced by tryptase(TRYP) were used to establish an intestinal barrier dysfunction model. Caco-2 cell monolayers from both functional and dysfunctional samples were treated with POG (30, 60 and 120 µg/mL) for 2, 8, 24, 36, 48 and 72h...
June 16, 2018: Biological & Pharmaceutical Bulletin
Jianhui Liu, Honggang Wang, Yi Li, Peiliang Shi, Jianfeng Gong, Lili Gu, Weiming Zhu, Jieshou Li
Recent studies suggested that excessive Th1/17 cells concomitant with regulatory T cell deficiency might play important roles in Crohn's disease. Anti-CD52 mAb, which aims on CD52 antigen on mature immunocytes, has both T cell depletion and immunosuppressive activities. In this study, we evaluated the therapeutic effects and possible mechanisms of anti-CD52 treatment on interleukin-10 deficient mouse. Anti-mouse CD52 mAb was administered to C3H.IL-10-/- mice intraperitoneally 20μg per week for 2 weeks. The disease activity index, body weight, the histological grading of colitis, and levels of TNF-α, IFN-γ, IL-17 and IL-6 in colon were quantified after treatment...
June 13, 2018: Biological & Pharmaceutical Bulletin
Xiong Zhang, Qi Ni, Ying Wang, Hong-Wei Fan, Yingbin Li
Temozolomide (TMZ) is currently the first-line drug used for clinical postoperative or non-surgical chemotherapy for glioma, but acquired and intrinsic resistance to TMZ limits its application. The anti-proliferative effect of formononetin on human glioma cells had been confirmed. To improve therapeutic effects of TMZ, we studied the effect of formononetin in combination with TMZ on C6 glioma cells. The anti-proliferative effect of C6 cells was tested by MTT assay, The synergy was evaluated by Chou-Talalay method...
May 29, 2018: Biological & Pharmaceutical Bulletin
Jeong-Min Hong, Jun-Kyu Shin, Jeom-Yong Kim, Min-Jung Jang, Sun-Kyu Park, Jong-Hoon Lee, Jung-Hyo Choi, Sun-Mee Lee
Chrysanthemum zawadskii var. latilobum (CZ) has been used as a traditional medicine in Asian countries for the treatment of inflammatory diseases. Recently, CZ extract was shown to inhibit differentiation of osteoclasts and provide protection against rheumatoid arthritis. The aim of this study was to investigate the molecular mechanisms of BST106, the ethanol extract of CZ, for cartilage protection in monosodium iodoacetate (MIA)-induced osteoarthritis (OA), particularly focusing on apoptosis and autophagy...
May 23, 2018: Biological & Pharmaceutical Bulletin
Chikako Shimizu, Yutaka Mitani, Youichi Tsuchiya, Toshitaka Nabeshima
Ethanol (EtOH) dosage, frequency, and paired associative learning affect the risk of alcoholism. Recently, Spanagel et al. reported that acamprosate calcium (Acam Ca) prescribed for alcoholism exerts an anti-relapse effect via Ca. Ca is contained in foods, sometimes consumed with alcohol. Therefore, we investigated the association among oral Ca ingestion, EtOH-induced locomotor sensitization, and plasma Ca levels on how to consume Ca for moderate drinking. We used DBA/2 CrSlc mice, and CaCl2 as water-soluble Ca salts...
May 16, 2018: Biological & Pharmaceutical Bulletin
Takehiro Yamada, Shungo Imai, Yasuyuki Koshizuka, Yuki Tazawa, Keisuke Kagami, Naoki Tomiyama, Ryosuke Sugawara, Akira Yamagami, Tsuyoshi Shimamura, Ken Iseki
Therapeutic drug monitoring for voriconazole, an antifungal agent, is essential for maximizing efficacy and preventing toxicity. The aim of this study was to elucidate the optimal maintenance dose of voriconazole in patients with severe liver cirrhosis (Child-Pugh class C) by reviewing the plasma trough concentrations obtained by therapeutic drug monitoring and daily doses of voriconazole. We retrospectively evaluated 6 patients with Child-Pugh class C cirrhosis who received oral voriconazole treatment and were liver transplant recipients or were awaiting liver transplantation...
May 11, 2018: Biological & Pharmaceutical Bulletin
Masayuki Saito, Masayoshi Ajioka, Takahiro Iwao, Tadashi Suzuki
The pharmacokinetics and pharmacodynamics of warfarin remained unaffected by tolvaptan during clinical trials. However, tolvaptan prolonged the prothrombin time-international normalized ratio (PT-INR) level of patients with cardiovascular disease taking warfarin. Tolvaptan was prescribed to 576 patients from December 2010 to December 2015. Of these patients, 37 underwent anticoagulant therapy. We investigated PT-INR fluctuation immediately before tolvaptan therapy was initiated. PT-INR remained unchanged in the control group and in groups administered with less than 7...
May 11, 2018: Biological & Pharmaceutical Bulletin
Min Liang, Yuru Du, Wenjing Li, Xi Yin, Ni Yang, Anran Qie, Tyler W LeBaron, Jiayu Zhang, Haiying Chen, Haishui Shi
SuHeXiang (SHX) has been used to treat a wide range of diseases, including those related to the central nervous system. However, the effects of SHX on mood disorders are still elusive. This study aimed to investigate the effects of SHX essential oil on stress-induced depression of mice. In an acute stress-induced depression model, mice inhaled vehicle (1% Tween80) for 10 min or 10% SHX for 10 or 30 min once daily for 12 continuous days. In the chronic mild stress (CMS)-induced depression model, mice were exposed to a 28-d CMS treatment...
May 9, 2018: Biological & Pharmaceutical Bulletin
Qi Wang, Shudong Liu, Aihua Zhai, Bai Zhang, Guizhen Tian
AMP-activated protein kinase (AMPK) is a metabolic sensor in mammals that is activated when ATP levels in the cell decrease. AMPK is a heterotrimeric protein that comprises 3 subunits, each of which has multiple phosphorylation sites that play critical roles in the regulation of either anabolism or catabolism by directly phosphorylating proteins or modulating gene transcription in multiple pathways, such as synthesis, oxidation and lipolysis of lipid. Research focused on the phosphorylation sites that are involved in lipid metabolism will lead to a better recognition of the role of AMPK in therapeutics for several common diseases...
April 28, 2018: Biological & Pharmaceutical Bulletin
Shun Guo, Song Zhang, Linna Liu, Peng Yang, Xueliang Dang, Huamei Wei, Na Hu, Lei Shi, Yan Zhang
Pinelliae Rhizoma Praeparatum (PRP) as traditional Chinese medicine had been used for hepatic diseases in combinative forms. However, the effect of PRP was not clear when used alone. So to explore the hepatoprotective/hepatotoxin of PRP is necessary. The activities of PRP were investigated in acetaminophen-induced hepatic injury mice. Liver function markers, hepatic oxidative stress markers were evaluated. Bile acids metabolic transports and nuclear factor erythroid 2-related factor 2 (Nrf2) were detected. As a drug for the treatment of liver diseases, PRP slightly restored the parameters towards normal in model mice only in low dosage, and also had no antioxidant activity and regulate Nrf2...
June 1, 2018: Biological & Pharmaceutical Bulletin
Zhen-Zhen Yuan, You-Rui Suo, Xue-Yan Hao, Shu-Lin Wang, Gang Li, Hong-Lun Wang
Six triterpenic acids were separated and purified from the ethyl acetate extractive fraction of ethanol extracts of Potentilla parvifolia FISCH. using a variety of chromatographic methods. The neuroprotective effects of these triterpenoids were investigated in the present study, in which the okadaic acid induced neurotoxicity in human neuroblastoma SH-SY5Y cells were used as an Alzheimer's disease cell model in vitro. The cell model was established with all trans-retinoic acid (5 µmol/L, 4 d) and okadaic acid (40 nmol/L, 6 h) treatments to induce tau phosphorylation and synaptic atrophy...
June 1, 2018: Biological & Pharmaceutical Bulletin
Airi Ohsaki, Yuki Miyano, Rei Tanaka, Sei-Ichi Tanuma, Shuji Kojima, Mitsutoshi Tsukimoto
Skin inflammation is caused by excessive production of cytokines and chemokines in response to an external stimulus, such as radiation, but the mechanisms involved are not completely understood. Here, we report a novel mechanism of γ-irradiation-induced interleukin-6 (IL-6) production mediated by P2Y11 receptors in epidermal cells. After irradiation of HaCaT cells derived from human epidermal keratinocytes with 5 Gy of γ-rays (137 Cs: 0.78 Gy/min), IL-6 production was unchanged at 24 h after γ-irradiation, but was increased at 48 h...
June 1, 2018: Biological & Pharmaceutical Bulletin
Mai Hazekawa, Kazuhiko Ono, Takuya Nishinakagawa, Tomoyo Kawakubo-Yasukochi, Manabu Nakashima
Sodium 4-phenylbutyrate (PBA), which exerts a wide range of anti-inflammatory effects, is rapidly cleared from the body (approximately 98%) by urinary excretion by 24 h after oral treatment in humans. PBA was almost entirely excreted to urine as phenylacetyl glutamine (PAGln). However, no data describe the potential anti-inflammatory effects of PAGln. The purpose of this study was to evaluate the anti-inflammatory effects of PAGln on mouse spleen cells and peritoneal cavity cells, and explore the potential mechanism underlying this effect...
June 1, 2018: Biological & Pharmaceutical Bulletin
Tetsusuke Yoshimoto, Emi Ryu, Shiro Tomiyasu, Minoru Hojo, Hideya Kokubun, Motohiro Matoba
Pure oxycodone injection became increasingly necessary after oral oxycodone was launched in Japan in 2003. However, trials clarifying the efficacy and safety of injection are rare. Therefore, a multicenter open study on injection was designed and carried out in 2010, resulting in the launch of injection therapy in 2012. As published domestic case reports on efficacy already show widespread prescription, this study aimed to provide useful information for cancer pain relief in Japan and other countries. Our oxycodone injection study consisted of two trials, one of intravenous (S#9131) and the other of subcutaneous (S#9132) administration...
June 1, 2018: Biological & Pharmaceutical Bulletin
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