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Wound Repair and Regeneration

Emiliano Tognoli, Matte Leoni, Daniele Morelli, Elisa Sottotetti, Antonia Martinetti, Stefano Signoroni, Carlotta Galeone, Gianfranco Gallino
Unrestrained activation of the proteolytic systems in anastomotic tissue during repair has been implicated in the pathogenesis of anastomotic leakage. We hypothesized that this mechanism may promote an up-regulation of the urokinase-type plasminogen activator system and a spillover of Soluble Urokinase-type Plasminogen Activator Receptor (suPAR) into blood. In this retrospective analysis patients with anastomotic leakage were compared to a group of matched uncomplicated patients. Anastomotic leakage complicated patients had significantly higher suPAR (p=0...
October 19, 2016: Wound Repair and Regeneration
Janelle Yu, Suzanne Lu, Ann-Marie McLaren, Julie A Perry, Karen M Cross
Diabetic foot ulcers (DFUs) are a significant problem in an aging population. 15% of diabetics develop a DFU over their lifetime, which can lead to potential amputation. The 5-year survival rate after amputation is 31%, which is greater than the lifetime risk of mortality from cancer. Topical oxygen is a promising technique for the adjunctive therapy of chronic wounds including DFUs, but few controlled studies exist to support its clinical adoption. The aim of this study was to compare a portable topical oxygen delivery system in patients with non-healing DFUs to standard best practice...
October 12, 2016: Wound Repair and Regeneration
Lampros Liasis, George Malietzis, George Galyfos, Thanos Athanasiou, Harry T Papaconstantinou, Fragiska Sigala, Georgios Zografos, Konstantinos Filis
INTRODUCTION: Lower limb ischaemia in diabetic patients is a result of macro- and microcirculation dysfunction. Diabetic patients undergoing limb amputation carry high mortality and morbidity rates, and decision making concerning the level of amputation is critical. Aim of this study is to evaluate a novel microdialysis technique in order to monitor tissue microcirculation preoperatively and predict the success of limb amputation in such patients. MATERIALS AND METHODS: Overall, 165 patients with type II diabetes mellitus undergoing lower limb amputation were enrolled...
October 12, 2016: Wound Repair and Regeneration
J Rothenberger, W Petersen, H E Schaller, M Held
A universal protocol determining the number of leeches and their application time does not exist. The aim of this study, therefore, is to quantify perfusion dynamics in venous congested tissues after leech application to get more detailed information about changes due to leech-induced skin microcirculation and to evaluate the usability of the O2C device in terms of determining the appropriate number of leeches and the duration of therapy. Twelve patients with the need for leech therapy participated in the study...
October 12, 2016: Wound Repair and Regeneration
Mingke Jiao, Lin Lou, Lin Jiao, Jie Hu, Peng Zhang, Zhongming Wang, Wenjuan Xu, Xiliang Geng, Hongping Song
Plateau frostbite (PF) treatments have remained a clinical challenge because this condition injures tissues in deep layers and affected tissues exhibit unique pathological characteristics. For instance, low-frequency pulsed electromagnetic field (PEMF) can affect tissue restoration and penetrate tissues. Therefore, the effect of PEMF on PF healing should be investigated. This study aimed to evaluate the effects of low-frequency PEMF on PF healing systematically. Ninety-six Sprague-Dawley rats were randomly and equally divided into three groups: normal control, partial thickness plateau frostbite (PTPF), and PTPF with low-frequency PEMF exposure (PTPF + PEMF)...
September 29, 2016: Wound Repair and Regeneration
Ana Flávia Marçal Pessoa, Juliana Costa Florim, Hosana Gomes Rodrigues, Vinicius Andrade-Oliveira, Simone A Teixeira, Kaio Fernando Vitzel, Rui Curi, Niels Olsen Saraiva Câmara, Marcelo N Muscará, Marcelo Lazzaron Lamers, Marinilce Fagundes Santos
Oxidative stress aggravates several long-term complications in diabetes mellitus. We evaluated the effectiveness of the oral administration of antioxidants (vitamins E and C, 40 and 100 mg/kg b.w., respectively) on skin wound healing acceleration in alloxan-induced diabetic mice. Mice were wounded 30 days after the induction of diabetes. Antioxidants were effective in preventing oxidative stress, as assessed by TBARS. The enzymes catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase were increased in diabetics on the 3rd day post-wounding; catalase and glutathione peroxidase remained still augmented in diabetics after 14th day postwounding, and the treatment with vitamins restored their activities to control...
September 29, 2016: Wound Repair and Regeneration
Louise L Dunn, Sarra de Valence, Jean-Christophe Tille, Philippe Hammel, Beat H Walpoth, Roland Stocker, Beat A Imhof, Marijana Miljkovic-Licina
Three-dimensional biomimetic scaffolds resembling the native extracellular matrix (ECM) are widely used in tissue engineering, however they often lack optimal bioactive cues needed for acceleration of cell proliferation, neovascularization, and tissue regeneration. In this study, the use of the ECM-related protein Olfactomedin-like 3 (Olfml3) demonstrates the importance and feasibility of fabricating efficient bioactive scaffolds without in vitro cell seeding prior to in vivo implantation. First, in vivo proangiogenic properties of Olfml3 were shown in a murine wound healing model by accelerated wound closure and a 1...
September 29, 2016: Wound Repair and Regeneration
Dominik André-Lévigne, Ali Modarressi, Rodrigue Pignel, Marie-Luce Bochaton-Piallat, Brigitte Pittet-Cuénod
The treatment of chronic wounds remains inconsistent and empirical. Hyperbaric oxygen therapy (HBOT) is a promising method to improve wound repair but there is still a lack of understanding of its mechanisms of action and its indications are not yet clearly defined. We studied the effects of HBOT in four different wound conditions by inflicting bilateral wounds on the dorsal aspect of the feet of nonischemic or ischemic limbs in normoglycemic or hyperglycemic rats. To create an ischemic condition, arterial resection was performed unilaterally...
September 29, 2016: Wound Repair and Regeneration
Christa J Bodaan, Lyn M Wise, Kirsty A Wakelin, Gabriella S Stuart, Nicola C Real, Andrew A Mercer, Christopher B Riley, Christine Theoret
Healing is delayed in limb wounds relative to body wounds of horses, partly because of sustained inflammation and inefficient angiogenesis. In laboratory animals, proteins derived from orf virus modulate these processes and enhance healing. We aimed to compare immune cell trafficking and the inflammatory, vascular and epidermal responses in body and limb wounds of horses, and then to investigate the impact of orf virus interleukin-10 and vascular endothelial growth factor-E on these processes. Standardized excisional wounds were created on the body and forelimb of horses and their progression monitored macroscopically until healed...
September 29, 2016: Wound Repair and Regeneration
Jeremy S Honaker, Michael R Forston, Emily A Davis, Michelle M Weisner, Jennifer A Morgan, Emily Sacca
The optimal treatment for deep tissue pressure injuries has not been determined. Deep tissue pressure injuries represent a more ominous early stage pressure ulcer that may evolve into full thickness ulceration despite implementing the standard of care for pressure ulcers. A longitudinal prospective historical case control study design was used to determine the effectiveness of non-contact low frequency ultrasound plus standard of care (treatment group) in comparison to standard of care (control group) in reducing deep tissue pressure injury severity, total surface area, and final pressure injury stage...
September 27, 2016: Wound Repair and Regeneration
Vickie R Driver, Kristen A Eckert, Marissa J Carter, Michael A French
This study analyzed a cross-section of patients with severe chronic wounds and multiple comorbidities at an outpatient wound clinic, with regard to the cost-effectiveness and cost-benefit of negative pressure wound therapy (intervention) versus no negative pressure wound therapy (control) at 1 and 2 years. Medicare reimbursement charges for wound care were used to calculate costs. Amputation charges were assessed using diagnosis-related groups. Cost-benefit analysis was based on ulcer-free months and cost-effectiveness on quality-adjusted life-years...
September 27, 2016: Wound Repair and Regeneration
Ching-Yan C Yeung, David F Holmes, Helen A Thomason, Christian Stephenson, Brian Derby, Matthew J Hardman
Pressure ulcers are complex wounds caused by pressure- and shear-induced trauma to skin and underlying tissues. Pressure-reducing devices, such as dressings, have been shown to successfully reduce pressure ulcer incidence, when used in adjunct to pressure ulcer preventative care. While pressure-reducing devices are available in a range of materials, with differing mechanical properties, understanding of how a material's mechanical properties will influence clinical efficacy remains limited. The aim of this study was to establish a standardised ex vivo model to allow comparison of the cell protection potential of two gel-like pressure-reducing devices with differing mechanical properties (elastic moduli of 77 kPa v 35 kPa)...
September 26, 2016: Wound Repair and Regeneration
Joris A van Dongen, Hieronymus P Stevens, Mojtaba Parvizi, Berend van der Lei, Martin C Harmsen
Autologous adipose tissue transplantation is clinically used to reduce dermal scarring and to restore volume loss. The therapeutic benefit on tissue damage more likely depends on the stromal vascular fraction of adipose tissue than on the adipocyte fraction. This stromal vascular fraction can be obtained by dissociation of adipose tissue, either enzymatically or mechanical. Enzymatic dissociation procedures are time-consuming and expensive. Therefore, we developed a new inexpensive mechanical dissociation procedure to obtain the stromal vascular fraction from adipose tissue in a time sparing way, which is directly available for therapeutic injection...
September 26, 2016: Wound Repair and Regeneration
Kehua Zhou, Peng Jia
Depression slows wound healing in patients with chronic wounds. The prevalence of depressive symptoms differs in the literature and the current understandings of factors related to depression in patients with wounds have been limited. To investigate the prevalence of depressive symptoms and the associated factors in patients with wounds, we performed this retrospective study in which depressive symptoms were evaluated with the Patient Health Questionnaire 9-item (PHQ-9). Valid PHQ-9 scores were collected from 222 patients (112 males and 110 females; age: 64...
September 26, 2016: Wound Repair and Regeneration
Andrew Baird, Chenliang Deng, Matthew H Eliceiri, Fatima Haghi, Xitong Dang, Raul Coimbra, Todd W Costantini, Bruce E Torbett, Brian P Eliceiri
Mice engrafted with human CD34(+) hematopoietic stem and progenitor cells (CD34(+) -HSPCs) have been used to study human infection, diabetes, sepsis, and burn, suggesting that they could be highly amenable to characterizing the human inflammatory response to injury. To this end, human leukocytes infiltrating subcutaneous implants of polyvinyl alcohol (PVA) sponges were analyzed in immunodeficient NSG mice reconstituted with CD34(+) -HSPCs. It was reported that human CD45(+) (hCD45(+) ) leukocytes were present in PVA sponges 3 and 7 days postimplantation and could be localized within the sponges by immunohistochemistry...
September 24, 2016: Wound Repair and Regeneration
Kristo Nuutila, Mansher Singh, Carla Kruse, Justin Philip, Edward J Caterson, Elof Eriksson
Standardized and reproducible animal models are crucial in medical research. Rodents are commonly used in wound healing studies since, they are easily available, affordable and simple to handle and house. However, the most significant limitation of rodent models is that the wounds heal by contraction while in humans the primary mechanisms of healing are reepithelialization and granulation tissue formation. The robust contraction results in faster wound closure that complicates the reproducibility of rodent studies in clinical trials...
September 8, 2016: Wound Repair and Regeneration
Liang Liang, Rivka C Stone, Olivera Stojadinovic, Horacio Ramirez, Irena Pastar, Anna G Maione, Avi Smith, Vanessa Yanez, Aristides Veves, Robert S Kirsner, Jonathan A Garlick, Marjana Tomic-Canic
Diabetic foot ulcers (DFUs) are one of the major complications of diabetes. Its molecular pathology remains poorly understood, impeding the development of effective treatments. Although it has been established that multiple cell types, including fibroblasts, keratinocytes, macrophages, and endothelial cells, all contribute to inhibition of healing, less is known regarding contributions of individual cell type. Thus, we generated primary fibroblasts from nonhealing DFUs and evaluated their cellular and molecular properties in comparison to nondiabetic foot fibroblasts (NFFs)...
September 8, 2016: Wound Repair and Regeneration
Hye One Kim, Yong Se Cho, Sook Young Park, In Suk Kwak, Min Gyu Choi, Bo Young Chung, Chun Wook Park, Jun Young Lee
Post burn pruritus is a common distressing sequela of burn wounds. Empirical antipruritic treatment often fails to have a satisfactory outcome, as the mechanism of it has not been fully elucidated. The aim of this study was to evaluate the manifestation of transient receptor potential vanilloid 3 (TRPV3), transient receptor potential ankyrin 1 (TRPA1) and other related receptors in post burn pruritus. Sixty-five burn patients with (n = 40) or without (n = 25) pruritus were investigated, including skin biopsies...
August 19, 2016: Wound Repair and Regeneration
Yoshikiyo Akasaka
No abstract text is available yet for this article.
August 8, 2016: Wound Repair and Regeneration
Robert S Kirsner, Wolfgang Vanscheidt, David H Keast, John C Lantis, Cyaandi R Dove, Shawn M Cazzell, Mher Vartivarian, Matthias Augustin, William A Marston, Nicholas D McCoy, D Innes Cargill, Tommy D Lee, Jaime E Dickerson, Herbert B Slade
In 2012 we reported promising results from a Phase 2 clinical trial of HP802-247, a novel spray-applied investigational treatment for chronic venous leg ulcers (VLU) consisting of human, allogeneic fibroblasts and keratinocytes. We now describe Phase 3 clinical testing of HP802-247, its failure to detect efficacy, and subsequent investigation into the root causes of the failure. Two randomized, controlled trials enrolled a total of 673 adult outpatients at 96 centers in North America and Europe. The primary endpoint was the proportion of ulcers with confirmed closure at the end of 12 weeks of treatment...
August 6, 2016: Wound Repair and Regeneration
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