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Wound Repair and Regeneration

Gregory Schultz, Thomas Bjarnsholt, Garth A James, David J Leaper, Andrew J McBain, Matthew Malone, Paul Stoodley, Terry Swanson, Masahiro Tachi, Randall D Wolcott
BACKGROUND: Despite a growing consensus that biofilms contribute to a delay in the healing of chronic wounds, conflicting evidence pertaining to their identification and management can lead to uncertainty regarding treatment. This, in part, has been driven by reliance on in vitro data or animal models, which may not directly correlate to clinical evidence on the importance of biofilms. Limited data presented in human studies have further contributed to the uncertainty. Guidelines for care of chronic wounds with a focus on biofilms are needed to help aid the identification and management of biofilms, providing a clinical focus to support clinicians in improving patient care through evidence-based medicine...
September 29, 2017: Wound Repair and Regeneration
Alexander S Girich, Marina P Issaeva, Igor Yu Dolmatov
Several genes of the Wnt and Frizzled families in the holothurian Eupentacta fraudatrix are characterized, and the complete coding sequences of wntA, wnt4, wnt6, wnt16, frizzled1/2/7, frizzled4, and frizzled5/8 are obtained. The dynamics of expression of these genes during regeneration of internal organs after evisceration are studied. Evisceration and the associated damages supposedly induce the expression of wnt16 on third day after evisceration. Genes wntA, wnt4, wnt6, and frizzled1/2/7 upregulate during the period of active morphogenesis (5-7 days after evisceration) and might participate in regulation of tissue and organ formation...
September 29, 2017: Wound Repair and Regeneration
Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Aiko Suzuki, Naoyuki Takagi, Hideki Yamamoto, Keiko Ishii, Yoshimichi Imai, Ryoko Maruyama, Masahiro Tachi
The wound-healing process consists of the inflammation, proliferation, and remodeling phases. In chronic wounds, the inflammation phase is prolonged with persistent neutrophil infiltration. The inflammatory response is critically regulated by cytokines and chemokines that are secreted from various immune cells. Recently, we showed that skin wound healing was delayed and the healing process was impaired under conditions lacking invariant natural killer T (iNKT) cells, an innate immune lymphocyte with potent immuno-regulatory activity...
September 23, 2017: Wound Repair and Regeneration
Ruxandra F Sîrbulescu, Chloe K Boehm, Erin Soon, Moses Q Wilks, Iulian Ilieş, Hushan Yuan, Ben Maxner, Nicolas Chronos, Charalambos Kaittanis, Marc D Normandin, Georges El Fakhri, Dennis P Orgill, Ann E Sluder, Mark C Poznansky
Chronic wounds affect 12-15% of patients with diabetes and are associated with a drastic decrease in their quality of life. Here we demonstrate that purified mature naïve B220(+) /CD19(+) /IgM(+) /IgD(+) B cells improve healing of acute and diabetic murine wounds after a single topical application. B cell treatment significantly accelerated acute wound closure by 2-3 days in wild-type mice and 5-6 days in obese diabetic mice. The treatment led to full closure in 43% of chronic diabetic wounds, as compared to only 5% in saline-treated controls...
September 18, 2017: Wound Repair and Regeneration
Inmaculada Lupiañez-Pérez, Juan Carlos Morilla-Herrera, Shakira Kaknani-Uttumchanchandani, Yolanda Lupiañez-Perez, Magdalena Cuevas-Fernandez-Gallego, Francisco Martin-Santos, Jorge Caro-Bautista, Jose Miguel Morales-Asencio
Pressure ulcers represent a major current health problem and cause an important economic impact on the health care system. Most studies on the prevention of pressure ulcers have been carried out in hospital contexts, with respect to the use of hyperoxygenated fatty acids, and to date no studies have specifically examined the use of olive oil-based treatments. AIM: To evaluate the cost of using extra virgin olive oil, rather than hyperoxygenated fatty acids, in the prevention of pressure ulcers among persons with impaired mobility and receiving home care...
September 18, 2017: Wound Repair and Regeneration
Christina L Rettinger, John L Fletcher, Anders H Carlsson, Rodney K Chan
Split-thickness skin grafting (STSG) is the current gold standard for treatment of extensive burn and traumatic skin injuries. However, STSG is limited by donor-site morbidity and availability, and often leads to scarring and wound contracture. Furthermore, these thin grafts lack dermal elements such as nerves and adnexa which are important in recapitulating normal skin function. Methods of fractional skin replacement either as minced STSGs or microscopic skin tissue columns have been proposed, though these techniques have not been fully characterized and lack evidence of regenerated adnexal structures...
September 18, 2017: Wound Repair and Regeneration
Michael Stacey, Steven Phillips, Forough Farrokhyar, Jillian M Swaine
Area measurements of a chronic wound are the gold standard outcome measure to determine if a wound is on a healing or non-healing trajectory. The use of digital planimetry can provide increased accuracy in measuring wound area however it is important to know the reliability and measurement error of these devices when used by multiple assessors. The aim of this study is to determine the within rater, between rater, and standard error of measurement of a digital planimetry device. Wound area in 42 patients was measured weekly for 12 weeks by two different raters, with each rater measuring the wound 10 times per visit...
September 18, 2017: Wound Repair and Regeneration
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, that is, the decantation and Coleman's centrifugation techniques...
September 14, 2017: Wound Repair and Regeneration
Sik Namgoong, Seung-Kyu Han
In spite of a period of intense economic, social, and political hardship following the Korean War, the Republic of Korea has made great strides in economic growth over the past half century, and also remarkable progress in the medical field. However, wound research has been slow to take off in South Korea. Meanwhile, as is the case in many countries in Western Europe and North America, South Korea is seeing a steady increase in its aged population. The growth in the geriatric population has in turn brought on more cases of chronic wounds...
August 30, 2017: Wound Repair and Regeneration
Jihong Cui, Hanxue Zheng, Jing Zhang, Liyuan Jia, Yalong Feng, Wenjun Wang, Hongmin Li, Fulin Chen
Glycans are known to play important roles in molecular recognition, cell-cell adhesion, molecular trafficking, receptor activation, and signal transduction during development and regeneration. Despite numerous investigations of regenerating salamander limbs, global analysis of the precise variation of glycans during the limb regeneration process has received little attention. Here, we have used lectin microarrays and lectin histochemistry to analyze the alterations and distribution of glycans during the early stages leading to blastema formation during Cynops orientalis limb regeneration in response to limb amputation...
August 30, 2017: Wound Repair and Regeneration
Rachel Sommer, Matthias Augustin, Carsten Hampel-Kalthoff, Christine Blome
The Wound-QoL questionnaire has been tested in a previous study, indicating that the instrument is a reliable and valid measurement for the assessment of HRQoL in chronic wounds. The aim of this study was to examine the test-retest reliability of the Wound-QoL. Patients were asked to complete the Wound-QoL twice within 3-7 days. Next to descriptive statistics, internal consistency was determined. Test re-test reliability was calculated with the intraclass correlation coefficient. The data showed ICCs between 0...
August 30, 2017: Wound Repair and Regeneration
Min Ho Kim, Wen Hao Wu, Jee Hyun Choi, Ji Hyun Kim, Jin Hyun Jun, Yong Ko, Jong Hun Lee
Keratinocytes and fibroblasts cells play important roles in the skin-wound healing process and are the cell types activated by trauma. Activated cells participate in epithelialization, granulation, scar-tissue formation, wound remodeling, and angiogenesis via a series of cellular activities including migration and proliferation. Previous studies reported that the conditioned medium (CM) of adipose-derived stem cells (ADSCs) stimulated the migration and proliferation of cell types involved in the skin-wound healing process; however, these studies only show ADSC-CM effects that were obtained using 2-dimensional (2D) culture...
August 30, 2017: Wound Repair and Regeneration
Steven L Percival, Dieter Mayer, Anne-Marie Salisbury
The aim of this study was to evaluate the efficacy of both a nonantimicrobial and antimicrobial (1% silver sulfadiazine-SSD) surfactant-based wound dressing in the control of Pseudomonas aeruginosa, Enterococcus sp, Staphylococcus epidermidis, Staphylococcus aureus, and methicillin-resistant S. aureus (MRSA) biofilms. Anti-biofilm efficacy was evaluated in numerous adapted American Standards for Testing and Materials (ASTM) standard biofilm models and other bespoke biofilm models. The ASTM standard models employed included the Minimum biofilm eradication concentration (MBEC) biofilm model (ASTM E2799) and the Centers for Disease Control (CDC) biofilm reactor model (ASTM 2871)...
August 30, 2017: Wound Repair and Regeneration
Christine J Andrews, Roy M Kimble, Margit Kempf, Leila Cuttle
Deep dermal burn injuries require extensive medical care; however, the water temperatures and durations of exposure that result in a severe scald injury are unknown. This study used a porcine burn model to investigate the time and temperature threshold for clinically relevant deep dermal injuries for both immersion (long duration) and spill/splash (short duration) scald events. Scald wounds were created on the flanks of anaesthetized juvenile large White pigs (27 kg). Acute tissue injury evaluations performed at 1 hour and days 1, 3, and 7 postburn (16 pigs) included: wound examination, biopsies, and laser Doppler imaging...
August 30, 2017: Wound Repair and Regeneration
William J Ennis, Rachel A Hoffman, Geoffrey C Gurtner, Robert S Kirsner, Hanna M Gordon
INTRODUCTION: Chronic wounds are increasing in prevalence and are a costly problem for the US healthcare system and throughout the world. Typically outcomes studies in the field of wound care have been limited to small clinical trials, comparative effectiveness cohorts and attempts to extrapolate results from claims data-bases. As a result, outcomes in real world clinical settings may differ from these published studies. OBJECTIVES: This study presents a modified intent-to-treat framework for measuring wound outcomes and measures the consistency of population based outcomes across two distinct settings...
August 28, 2017: Wound Repair and Regeneration
Liselotte Butzelaar, Frank B Niessen, Wendy Talhout, Dennis Pm Schooneman, Magda M Ulrich, Robert Hj Beelen, Aebele B Mink van der Molen
The purpose of this study was to examine extra cellular matrix composition, vascularization and immune cell population of skin sites prone to keloid formation. Keloids remain a complex problem, posing esthetical as well as functional difficulties for those affected. These scars tend to develop at anatomic sites of preference. Mechanical properties of skin vary with anatomic location and depend largely on extra cellular matrix composition. These differences in extra cellular matrix composition, but also vascularization and resident immune cell populations might play a role in the mechanism of keloid formation...
August 28, 2017: Wound Repair and Regeneration
Gijs H J de Smet, Leonard F Kroese, Anand G Menon, Johannes Jeekel, Antoon W J van Pelt, Gert-Jan Kleinrensink, Johan F Lange
Oxygen is an important factor for wound healing. Although several different therapies investigated the use of oxygen to aid wound healing, the results of these studies are not unequivocal. This systematic review summarizes the clinical and experimental studies regarding different oxygen therapies for promoting wound healing, and evaluates the outcomes according the methodological details. A systematic literature search was conducted using Embase, Medline, Web of Science, Cochrane, PubMed publisher, and Google Scholar libraries...
August 7, 2017: Wound Repair and Regeneration
Li Zhang, Changshui Weng, Zhanbo Zhao, Xiaobing Fu
A growing number of clinical studies demonstrate that extracorporeal shock wave therapy is a feasible noninvasive method for improving chronic wound healing. This systematic review and meta-analysis aimed to assess the effectiveness of extracorporeal shock wave therapy compared with that of the standard care treatment for the healing of chronic wounds, irrespective of etiology, in clinical practice. Randomized controlled trials that investigated the effect of extracorporeal shock wave therapy on chronic wounds with different etiologies from 2000 to 2017 were included in this review...
July 31, 2017: Wound Repair and Regeneration
Jodi C McDaniel, Laura Szalacha, Michelle Sales, Sashwati Roy, Scott Chafee, Narasimham Parinandi
Sustained high levels of activated polymorphonuclear leukocytes (PMNs) and PMN-derived proteases in the microenvironment of chronic venous leg ulcers (CVLUs) are linked to chronic inflammation and delayed healing. Uncontrolled PMN activity eventually destroys newly developed tissue and degrades critical growth factors. The bioactive components of fish oil (n-3 eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA]) have strong inflammation-resolving actions and have been shown to assuage PMN activity, but have not been tested in CVLU patients...
July 31, 2017: Wound Repair and Regeneration
Marissa J Carter
Publications based on large healthcare databases that contain data pertaining to wound-related outcomes are starting to appear more frequently. However, concern exists in regard to study design adequacy, the methodology used to minimize misclassifications, bias, and confounding, and lack of full reporting. The STROBE guidelines were published to encourage fuller reporting of observational studies and have now been extended using the RECORD statement to better document routinely collected healthcare data. In this paper, elements of the RECORD statement have been used to create guidelines for study design, cohort matching, reporting criteria, and analysis frameworks in regard to analyses of populations involving comparative effectiveness research...
July 29, 2017: Wound Repair and Regeneration
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