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Transplant Immunology

Jianxin Yang, Frans H J Claas, Michael Eikmans
Since the discovery of the human leukocyte antigen (HLA) system, the role of HLA molecules in the field of transplantation has been appreciated: better matching leads to better graft function. Since then, the association of other genetic polymorphisms with clinical outcome has been investigated in many studies. Genome-wide association studies (GWAS) represent a powerful tool to identify causal genetic variants, by simultaneously analyzing millions of single nucleotide polymorphisms scattered across the genome...
April 25, 2018: Transplant Immunology
Li Zhu, Mostafa Aly, Haihao Wang, Hristos Karakizlis, Rolf Weimer, Christian Morath, Ruben Jeremias Kuon, Bettina Toth, Naruemol Ekpoom, Gerhard Opelz, Volker Daniel
BACKGROUND: There is evidence that NK cells with low cytotoxicity but strong immunoregulatory characteristics contribute to good graft outcome. We attempted to investigate which NK cell subsets increase post-transplant and might affect graft function. METHOD: Lymphocyte and NK cell subsets were determined in whole blood using eight-colour-fluorescence flow cytometry in patients pre-transplant and post-transplant. In total, 31 transplant recipients were studied. RESULTS: When cell numbers were compared in 9 patients pre- and 6 months post-transplant, post-transplant CD56dimCD16+ (p = 0...
April 24, 2018: Transplant Immunology
P L J van der Heiden, H M van Egmond, S A J Veld, M van de Meent, M Eefting, L C de Wreede, C J M Halkes, J H F Falkenburg, W A F Marijt, I Jedema
BACKGROUND: Cytomegalovirus (CMV)-specific T-cells are crucial to prevent CMV disease. CMV seropositive recipients transplanted with stem cells from a CMV seronegative allogeneic donor (R+ D- ) may be at risk for CMV disease due to absence of donor CMV-specific memory T-cells in the graft. METHODS: We analyzed the duration of CMV reactivations and the incidence of CMV disease in R+ D- and R+ D+ patients after alemtuzumab-based T-cell depleted allogeneic stem cell transplantation (TCD alloSCT)...
April 18, 2018: Transplant Immunology
Xiaoming Zhang, Tongyi Men, Haitao Liu, Xianduo Li, Jianning Wang, Jiaju Lv
BACKGROUND: Post-transplantation diabetes mellitus (PTDM) is a serious metabolic complication after kidney transplantation. The aim of this study was to explore the association of clinical variables and five selected single nucleotide polymorphisms (SNPs) with PTDM in Chinese Han renal allograft recipients taking tacrolimus (TAC). METHODS: A total of 129 non-diabetic, primary, Chinese Han renal allograft recipients treated with TAC were enrolled. Five SNPs (CYP3A5 rs776741, rs776746, rs15524, CYP24A1 rs2296241, and PPARG rs1801282) were genotyped and analyzed...
April 14, 2018: Transplant Immunology
Annika Gocht, Jörg H W Distler, Bernd Spriewald, Martina Ramsperger-Gleixner, Michael Weyand, Stephan M Ensminger, Christian Heim
BACKGROUND: Cardiac allograft vasculopathy (CAV) is the main obstacle for long-term survival after heart transplantation. Alloimmune mediated chronic vascular rejection results in several mechanisms like platelet activation, immigration of inflammatory cells through the endothelial layer and proliferation and migration of smooth muscle cells (SMCs). Serotonin (5-HT) promotes these processes via activation of 5-HT2 receptors. We hypothesized that inhibiting 5-HT2 receptors ameliorates the development of CAV...
April 9, 2018: Transplant Immunology
Zhiqiang Yang, Yujian Liu, Xiaolei Zhou
RNA interference (RNAi) plays a potential role in organ transplantation. Small hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn that can be used to silence the expression of a target gene. We constructed shRNA targeting on the cluster of differentiation 80 (CD80, B7-1) and the cluster of differentiation 86 (CD86, B7-2) and transfected it into dendritic cells (DCs). Fluorescence real-time PCR and flow cytometry confirmed the gene-silencing effect. Interleukin-2 (IL-2) mRNA expression level decreased in T cells that were cocultured with pB7-shRNA-transfected DCs...
March 26, 2018: Transplant Immunology
Helena B Cazarote, Silvia Shimakura, Joana S Valdameri, Fabiana L C Contieri, Cristina Q C von Glehn, Carlos M Aita, Michelle F Susin, Vanessa Santos Sotomaior, Renata Glehn-Ponsirenas
Detection of donor-specific antibodies (DSA) has improved the risk classification and post-transplant evaluation of kidney recipients. Moreover, assessment of DSA C1q-binding ability has been shown to improve the individual risk classification of transplant patients for allograft loss, especially when detected after transplantation. The aim of this study was to evaluate the additional clinical impact of C1q-binding DSA detection in a population that was extensively monitored for DSA and MFI alterations. Forty-two kidney allograft recipients were followed-up at multiple time points for up to 5 years after transplantation for the presence of anti-HLA DSA-IgG total...
March 26, 2018: Transplant Immunology
J Salman, K Jansson, Th Siemeni, W Sommer, A-K Knoefel, L Ahrens, T Nakagiri, F Ius, I Tudorache, B Kruse, S Thissen, D Jonigk, M Strüber, A Haverich, G Warnecke, M Avsar
No abstract text is available yet for this article.
March 26, 2018: Transplant Immunology
Sandra A Carey, Kristen M Tecson, Aayla K Jamil, Joost Felius, Theresa K Wolf-Doty, Shelley A Hall
BACKGROUND: Serial gene expression profiling (GEP) may reduce the need for endomyocardial biopsies for detecting acute cellular rejection (ACR) after transplantation, but its performance in dual organ transplant recipients is currently unknown. METHODS: We analyzed 18 months of follow-up in a national cohort of 27 dual organ recipients (18 heart-kidney, 8 heart-liver, 1 heart-lung) matched to 54 heart-only recipients for gender, age, and time to first GEP (AlloMap®) test...
March 24, 2018: Transplant Immunology
Beatriz Chamun Gil, Adriane Stefani Silva Kulzer, Priscila de Moraes, Realdete Toresan, Alessandra da Rosa Vicari, Iara Dos Santos Fagundes, Jóice Merzoni, Gisele Menezes Ewald, Jacqueline Moraes Cardone, Fernanda Gamio Silva, Roberto Ceratti Manfro, Luiz Fernando Jobim
Preformed anti-human leukocyte antigen (HLA) antibodies may be present in the blood of kidney transplant candidates. The production of these antibodies may occur in the post-transplant period, with the possible development of donor-specific antibodies (DSA). Luminex-based tests, such as the single antigen (SA) assay and the Luminex crossmatch (Xm-DSA) assay are the most commonly used tools to detect anti-HLA antibodies, due to their high sensitivity and specificity. This cross-sectional study aimed to compare the findings of two methods for the detection of DSAs after kidney transplant: SA and Xm-DSA...
March 22, 2018: Transplant Immunology
Arianne D Pieterse, Volkert A L Huurman, Beerend P Hierck, Marlies E J Reinders
No abstract text is available yet for this article.
March 18, 2018: Transplant Immunology
Geisiane Custódio, Patrícia Schwarz, Daisy Crispim, Rafael B Moraes, Mauro Czepielewski, Cristiane B Leitão, Tatiana H Rech
BACKGROUND: Vitamin D insufficiency is linked to several common inflammatory disorders. Brain death (BD) causes a massive catecholamine release, leading to intense inflammatory activity. We aimed to evaluate vitamin D serum levels in brain-dead individuals in comparison to critically ill patients without BD to assess the correlation between vitamin D and cytokine levels. METHODS: Sixteen brain-dead patients and 32 critically ill controls were prospectively enrolled...
February 28, 2018: Transplant Immunology
Nozomi Aibara, Kaname Ohyama, Masaaki Hidaka, Naoya Kishikawa, Yasuyoshi Miyata, Mitsuhisa Takatsuki, Susumu Eguchi, Naotaka Kuroda
Liver transplantation is a life-saving procedure for many end-stage liver diseases; however, rejection after transplantation is still occurs in some recipients. The most common form of rejection is T cell-related acute cellular rejection (ACR). To understand the mechanism of rejection, it is necessary to identify immune targets. Since the development of B cell immunity depends upon concordant T cell immunity, we hypothesized that rejection-specific antigens in circulating immune complexes (CICs) may be present in the sera of recipients experiencing rejection, and as such, may be useful as diagnostic biomarkers for ACR...
February 27, 2018: Transplant Immunology
Yihang Jiang, Sujuan Feng, Jiawei Ji, Zhemin Lin, Xiaodong Zhang
INTRODUCTION: Post-infectious immunosuppression is disadvantageous to patients' long-term outcomes, especially in transplant recipients receiving large doses of immunosuppressants. A growing body of evidence indicates the immunoregulatory ability of myeloid-derived suppressor cells (MDSCs). We herein investigate the characteristics of monocytic-MDSCs (M-MDSCs) in a cohort of renal transplant recipients with/without infection to clarify the potential involvement in post-infectious immunosuppression...
February 22, 2018: Transplant Immunology
Yan Hong, Xiang-Yu Zhao, Xing-Xing Yu, Zhi-Lei Bian, Ying-Jun Chang, Yu Wang, Xiao-Hui Zhang, Lan-Ping Xu, Xiao-Jun Huang, Xiao-Su Zhao
INTRODUCTION: Invariant natural killer T cells (iNKTs) are a rare but vital subset of immunomodulatory T cells and play an important role in allogeneic hematopoietic stem cell trans-plantation (HSCT). The association of donor characteristics with the number and frequency of the iNKTs subsets in allografts remains poorly understood. In this paper, we prospectively investigate the association of donor characteristics with iNKTs dose and frequency in granulocyte-colony-stimulating factor (G-CSF) mobilized marrow and peripheral blood harvests...
February 21, 2018: Transplant Immunology
Benjamin Coiffard, Davide Piloni, Mohamed Boucekine, Monica Morosini, Federica Meloni, Romain Kessler, Martine Reynaud-Gaubert
No abstract text is available yet for this article.
February 21, 2018: Transplant Immunology
Jianxin Yang, Malou L H Snijders, Geert W Haasnoot, Cees van Kooten, Marko Mallat, Johan W de Fijter, Marian C Clahsen-van Groningen, Frans H J Claas, Michael Eikmans
BACKGROUND: Molecules of the innate immune response are increasingly recognized as important mediators in allograft injury during and after kidney transplantation. We therefore aimed to establish the relationship between the expression of these genes at implantation, during an acute rejection (AR) and on graft outcome. METHODS: A total of 19 genes, including Toll like receptors (TLRs), complement components and regulators, and apoptosis-related genes were analyzed at the mRNA level by qPCR in 123 biopsies with acute rejection and paired pre-transplantation tissue (n = 75)...
February 20, 2018: Transplant Immunology
Shang Zhang, Haitao Wu, Changlin Liu
It has been demonstrated that mesenchymal stem cells (MSCs) have potent immunosuppressive capacities. But it is controversial whether differentiated mature stromal cells (SCs) share the immunosuppressive capacities. A previous study examined the ability of SCs from different human tissue sites to inhibit the proliferation of lymphocytes. The results are all positive but the mechanism isn't clear, and few mouse data have been published on this topic. Using an efficient mixed cell culture assay, our in vitro data show that the anti-proliferative ability of murine MSCs on lymphocytes is shared by mature murine SCs, i...
April 2018: Transplant Immunology
Senthil Kumar, Nihar Mohapatra, Deeplaxmi Purushottam Borle, Ashok Choudhury, Shashwat Sarin, Ekta Gupta
No abstract text is available yet for this article.
April 2018: Transplant Immunology
Patmika Jiaravuthisan, Akira Maeda, Chihiro Takakura, Han-Tang Wang, Rieko Sakai, Afifah Mohd Shabri, Pei-Chi Lo, Rei Matsuura, Tasuku Kodama, Hiroshi Eguchi, Hiroomi Okuyama, Shuji Miyagawa
OBJECTIVE: Surfactant protein D (SP-D), which is secreted mainly in the lung, is an oligometric C type lectin that promotes phagocytosis by binding to carbohydrates on microbial surfaces. SP-D can also bind SIRPα, leading to a decrease in cytokine production by monocytes/macrophages. In the present study, we examined the possibility that SP-D suppresses macrophage-mediated xenogeneic cytotoxicity, by creating a membrane-type SP-D. METHODS: The cDNA for the carbohydrate recognition domain (CRD) of human SP-D was switched to that of a membrane-type protein, collectin placenta 1 (CL-P1), with a Flag-tag...
April 2018: Transplant Immunology
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