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British Journal of Biomedical Science

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https://www.readbyqxmd.com/read/28521643/a-magnetic-nanoparticle-labeled-immunoassay-with-europium-and-samarium-for-simultaneous-quantification-of-serum-pepsinogen-i-and-ii
#1
J Fan, H Xiao, J Zhang, B Zhou, L Deng, Y Zhang, B Huang
OBJECTIVE: To develop a novel immunoassay for the simultaneous determination of serum pepsinogen I (PG I) and pepsinogen II (PG II) by combining established methods of time-resolved fluoroimmunoassay (TRFIA) and magnetic nanoparticles separation. MATERIALS AND METHODS: This new immunoassay method was characterised by immobilising primary antibodies on the surface of magnetic particles and labelled with stable fluorescent chelates of europium and samarium. RESULTS: Using magnetic nanoparticles, the TRFIA immunoassay exhibited broad dynamic assay ranges for PG I with detection limit of 0...
May 19, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28513273/the-clinical-value-of-vav3-in-peripheral-blood-for-predicting-lymphatic-metastasis-of-gastric-cancer
#2
B Tan, Y Li, C Wang, M Tan, L Fan, Q Zhao, D Wang, N Jia
BACKGROUND: Overexpression of Vav3, a gene involved in signal transduction, promotes invasion and inhibits apoptosis in several cancers. The clinical value of the protein product of this gene, Vav3, in the peripheral blood of gastric cancer patients is unknown. We hypothesised increased serum Vav3 that related to tissue levels and lymph node metastases. In addition, we further explored its clinical value in respect of linked molecules Rac-1, MMP-7 and ICAM-1 Methods: 120 gastric cancer patients who had radical surgery were enrolled...
May 17, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28504002/development-and-evaluation-of-a-novel-score-for-prediction-of-large-oesophageal-varices-in-patients-with-hepatitis-c-virus-induced-liver-cirrhosis
#3
K Farid, M M Omran, R E Farag, M M Arafa, T M Emran
OBJECTIVE: Variceal bleeding is one of the most common life-threatening complications of liver cirrhosis. This study aimed to develop and evaluate a predictive score, named Platelet count, Alpha fetoprotein (AFP) and Prothrombin-INR (PAP) for the prediction of large oesophageal varices and to compare PAP score with eight common liver fibrosis scores (AAR, APRI, GUCI, BRC score, Fibro-Alfa, FIB4, Lok and Fibro-Q) in patients with hepatitis C virus (HCV) induced liver cirrhosis. METHODS: A total of 277 patients with HCV-induced liver cirrhosis were evaluated by upper gastrointestinal endoscopy for presence of varices...
May 15, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28493763/fluorescent-pcr-detection-of-meca-in-drug-resistant-mrsa-a-methodological-study
#4
Y Cao, Y Hao, D Yang, B Pang, L Wang
No abstract text is available yet for this article.
May 11, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28367740/mir-199a-3p-downregulation-in-thyroid-tissues-is-associated-with-invasion-and-metastasis-of-papillary-thyroid-carcinoma
#5
Chengbiao Liu, Meiling Xing, Liping Wang, Kejun Zhang
BACKGROUND AND AIMS: miR-199a-3p may play an important role in tumour inhibition. The aim of the present study was to determine any link between miR-199a-3p expression in thyroid tissues and clinicopathologic features, as well as to assess potential usefulness of miR-199a-3p in prediction for invasion and metastasis of papillary thyroid carcinoma (PTC). METHODS: A total of 188 tissue samples (136 PTCs, 52 normal thyroid tissue) were collected. We measured the levels of miR-199a-3p with quantitative reverse-transcriptase PCR (RT-qPCR) in all subjects...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28367739/towards-understanding-clinical-campylobacter-infection-and-its-transmission-time-for-a-different-approach
#6
E Casey, E Fitzgerald, B Lucey
Campylobacter spp. are among the most commonly diagnosed causes of human infection. Methods for detection of the 29 campylobacter species have mainly focused on cultivation of the thermophilic species. More than 99% of clinical campylobacter isolates notified in the UK in the recent past have been from faecal samples and associated with gastroenteritis. Campylobacter enteritis notifications in temperate zones show a seasonal increase during the summer months with a sharp decrease in the winter months, a pattern which remains incompletely understood...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28367738/comparison-of-the-fast-track-diagnostics-respiratory-21-and-seegene-allplex-multiplex-polymerase-chain-reaction-assays-for-the-detection-of-respiratory-viruses
#7
Kevin Barratt, Trevor P Anderson, Jennifer A Fahey, Lance C Jennings, Anja M Werno, David R Murdoch
BACKGROUND: Real-time multiplex PCR assays are increasingly used for respiratory virus detection, and offer automated analysis in a closed tube system, but they have the disadvantage of low-throughput due to multiplexing limitations. In this study, the established fast-track respiratory 21 assay (FTD) (fast-track diagnostics, Junglinster Luxembourg) was compared to the new Seegene Allplex assay (Seegene) (Seegene Inc. Seoul, Korea) which offers greater multiplexing as multiple targets can be detected in each fluorescence channel...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28367737/circulating-levels-of-collagen-iii-and-mmp-1-in-patients-with-chronic-hepatitis-c-co-infected-with-hepatitis-b-virus
#8
Abdelfattah M Attallah, Mohamed El-Far, Mohamed F Ghaly, Mohamed M Omran, Mohamed S Albannan, Ahmed A Attallah, Tarek M Shoghey, Mohamed M Atrees, Mohamed S Elbendary, Khaled Farid
BACKGROUND: There is controversial data in the literature about the characteristics and features of dual hepatitis B and hepatitis C infection. This work is concerned with estimating the extent to which HBV could influence circulating levels of hepatitis C viral nonstructural-4 (HCV-NS4) in addition to some direct fibrosis markers in chronic hepatitis C. METHODS: Thirty-eight HCV mono-infected and 87 HCV/HBV co-infected patients constituted this study. Western-blot and ELISA were used for identifying HCV-NS4, hepatitis B surface antigen (HBsAg), collagen III and matrixmetalloproteinase-1 (MMP-1) in patients' sera...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28367736/expression-of-wt1-and-pax8-in-the-epithelial-tumours-of-malaysian-women-with-ovarian-cancer
#9
Anthony Rhodes, Narayanan Vallikkannu, Pailoor Jayalakshmi
BACKGROUND: Ovarian cancer is particularly lethal due to late stage at presentation. The subtypes behave differently with respect to their biology and response to treatment. Two recent markers reported to be useful in assisting in the diagnosis are WT1 and PAX8. Malaysia, with its multi-ethnic population provides an opportunity to study the expression of these biomarkers in ovarian cancer in the three most populous ethnicities in Asia and ascertain their usefulness in the diagnosis of ovarian carcinoma...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28281934/integron-occurrence-is-linked-to-reduced-biocide-susceptibility-in-multidrug-resistant-pseudomonas-aeruginosa
#10
Ashraf A Kadry, Fathy M Serry, Amira M El-Ganiny, Ahmed M El-Baz
OBJECTIVE: Integrons are gene acquisition systems commonly found in bacterial genomes that play a major role in the dissemination of resistance to antibiotics. This work aimed to study the relationship between the presence of integrons and the reduced susceptibility of multidrug-resistant (MDR) Pseudomonas aeruginosa isolates towards different groups of biocides. METHODS: The antimicrobial susceptibility patterns of 104 clinical isolates were determined against different antibiotics by the disk diffusion method...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28102104/fasting-urinary-calcium-to-creatinine-ratio-for-the-evaluation-of-calcium-nephrolithiasis-in-adults
#11
Lijing Sun, Zhiyong Guo, Jianping Shan, Gengru Jiang
No abstract text is available yet for this article.
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27718888/platelet-rich-plasma-for-tissue-regeneration-can-be-stored-at-room-temperature-for-at-least-five-days
#12
Gary W Moore, James C Maloney, Robert A Archer, Kerri L Brown, Katarzyna Mayger, Elaine S Bromidge, Mitra F Najafi
BACKGROUND: Autologous platelet-rich plasma (PRP) is gaining increasing use as a wound healing promoter in a variety of clinical settings, including dentistry. Fresh PRP is often used, necessitating daily draws. The present study investigates the possibility of using stored PRP without having to freeze it by storing PRP under variable conditions and assessing growth factor release as a surrogate marker of continued viability. METHODS: Freshly drawn PRP was stored in oxygen permeable and non-oxygen permeable containers under conditions of constant agitation with or without added prostaglandin, intermittent agitation and no agitation, over an 8-day period...
April 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28074676/potential-route-of-th17-treg-cell-dynamics-in-targeting-type-1-diabetes-and-rheumatoid-arthritis-an-autoimmune-disorder-perspective
#13
Suresh Kumar Karri, A Sheela
Cytokines, small secreted proteins, have a specific effect on the interactions and communications between cells. They play a pivotal role in the pathogenesis of autoimmune diseases. Factors in the breakdown of self-tolerance and the subsequent events leading to the induction of pathogenic responses remain unclear for most of the autoimmune diseases. Large numbers of studies have revealed a general scheme in which pro-inflammatory cytokines contribute to the initiation and propagation of autoimmune inflammation, whereas anti-inflammatory cytokines facilitate the regression of inflammation and thereby recovery from the disease...
January 11, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28206854/the-relationship-between-multidrug-resistance-protein-1-rs1045642-and-cholesterol-24-hydroxylase-rs754203-genes-polymorphism-with-type-2-diabetes-mellitus
#14
Saliha Rizvi, Syed Tasleem Raza, S Riyaz Mehdi, Zeba Siddiqi, Ale Eba, Farzana Mahdi
BACKGROUND: The involvement of genetic factors like gene polymorphisms has been found to contribute significantly to the development and progression of type 2 diabetes (T2DM). Thousands of single nucleotide polymorphisms in various genes have been found to be associated with risk of T2DM. The present study was aimed to investigate association of Multidrug resistance 1 (MDR1) (rs1045642) and CYP46A1 (rs754203) genes polymorphism with T2DM. SUBJECTS & METHODS: Study includes 333 subjects, 183 T2DM cases and 150 healthy controls...
January 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28206853/diagnostic-virology-and-patient-care-from-vaguely-interesting-to-vitally-important
#15
Sarah J Pitt, D Ian M Phillips
The existence of pathogenic viruses was inferred by experiments at the turn of the twentieth century. Key developments in detection of viruses, including electron microscopy and monolayer cell culture, were made in the middle of that century. However, in terms of patient care, the results from the virology laboratory often arrived the patient was 'better or dead'. The advent of molecular techniques, particularly polymerase chain reaction and more recently whole genome sequencing made timely and accurate diagnosis of viral infections feasible...
January 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28206852/british-journal-of-biomedical-science-in-2016-what-have-we-learned
#16
Andrew Blann
In 2016, the British Journal of Biomedical Science published 36 reports outlining specific advances in each of the various disciplines within biomedical science. These were one review, 25 original articles, 9 'In Brief' reports and one letter to the Editor. Of these, the majority were in blood science (5 in biochemistry, 7 in haematology and 2 in immunology) and infection science (8 in microbiology, 2 in virology) with a smaller number in cellular sciences (6 in cellular pathology and 2 in cytopathology). Three reports considered both biochemistry and immunology, while another reported an advance in the identification of chromosomal abnormalities...
January 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27477890/hypophosphataemia-and-parenteral-nutrition-biochemical-monitoring-incidence-and-outcomes
#17
En C Fung, Sasala R Wickramasinghe, Julia V Panteli, Martin A Crook
No abstract text is available yet for this article.
January 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27996693/assessment-of-semi-automated-nucleic-acid-testing-programme-in-a-regional-blood-transfusion-centre
#18
Kanjaksha Ghosh, Kanchan Kumar Mishra, Apeksha Trivedi, Sheetal Sosa, Krima Patel
BACKGROUND: Detection of human immunodeficiency virus type-1 (HIV-1), hepatitis-C (HCV) and hepatitis-B virus (HBV) in the blood donors is crucial. An efficient form of detection is nucleic acid testing (NAT) in blood screening. We assessed the suitability of commercial NAT testing in a developing country, focusing on the Altona RealStar assay and the method of Sacace Biotechnologies. METHODS: We have standardised and validated commercially available NAT kits with a semi-automated system for detection of HBV, HCV and HIV-1 in blood donations...
December 20, 2016: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27976989/a-breath-ammonia-analyser-for-monitoring-patients-with-end-stage-renal-disease-on-haemodialysis
#19
J Limeres, J F Garcez, J S Marinho, A Loureiro, M Diniz, P Diz
OBJECTIVE: Breath ammonia measurement has attracted increasing interest for clinical diagnosis and metabolic status monitoring. This pilot study aims to evaluate a simple detection method to measure breath ammonia levels in haemodialysis patients. MATERIALS AND METHODS: The study group comprised 44 adults undergoing haemodialysis and a control group of 44 age- and sex-matched individuals with a glomerular filtration rate >90 mL/min. To measure breath ammonia concentration, we designed a device based on that used to monitor atmospheric air, which uses a specific colorimetric tube...
December 15, 2016: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/27719329/abcb1-polymorphisms-and-steroid-treatment-in-children-with-idiopathic-nephrotic-syndrome
#20
Manal A Safan, Nesreen G Elhelbawy, Dina A Midan, Heba F Khader
BACKGROUND: The most common cause of nephrotic syndrome (NS) is idiopathic nephrotic syndrome (INS), also called nephrosis. Although most patients respond to steroid therapy, there is unequal response to treatment suggesting the involvement of genetic factors. The current study was conducted to evaluate the influence of two single nucleotide polymorphisms (SNPs) in ABCB1 (C3435T and C1236T) on the steroid treatment response in INS children. MATERIALS AND METHODS: Genotyping of ABCB1 C3435T and C1236T polymorphisms by real time PCR were conducted on 120 INS children, 80 steroid sensitive (SS) and 40 steroid resistant (SR)...
October 8, 2016: British Journal of Biomedical Science
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