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Biological Research

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https://www.readbyqxmd.com/read/30012208/protective-roles-of-autophagy-in-retinal-pigment-epithelium-under-high-glucose-condition-via-regulating-pink1-parkin-pathway-and-bnip3l
#1
Chengchi Huang, Hong Lu, Junyu Xu, Hongmin Yu, Xiaodan Wang, Xiaomei Zhang
BACKGROUND: Our study aimed to investigate the roles of autophagy against high glucose induced response in retinal pigment epithelium (ARPE-19 cells). METHODS: The morphological changes and reactive oxygen species (ROS) generation in ARPE-19 cells under high glucose treatment were respectively detected using the transmission electron microscopy and flow cytometry. The expression levels of Parkin, PINK1, BNIP3L, LC3-I and LC3-II in ARPE-19 cells received high glucose treatment were measured by western blot after pretreatment of carbonyl cyanide m-chlorophenylhydrazone (CCCP), 3-methyladenine (3-MA), N-acetyl cysteine (NAC) or cyclosporin A (CsA) followed by high glucose treatment...
July 16, 2018: Biological Research
https://www.readbyqxmd.com/read/29980225/electroacupuncture-ameliorate-learning-and-memory-by-improving-n-acetylaspartate-and-glutamate-metabolism-in-app-ps1-mice
#2
Ruhui Lin, Long Li, Yingzheng Zhang, Sheng Huang, Shangjie Chen, Jiao Shi, Peiyuan Zhuo, Hao Jin, Zuanfang Li, Weilin Liu, Zhifu Wang, Lidian Chen, Jing Tao
OBJECTIVE: To explore the precise mechanism of electroacupuncture (EA) to delay cognitive impairment in Alzheimer disease. METHODS: N-Acetylaspartate (NAA), glutamate (Glu) and myoinositol (mI) metabolism were measured by magnetic resonance spectroscopy, learning and memory of APP/PS1 mouse was evaluated by the Morris water maze test and the step-down avoidance test, neuron survival number and neuronal structure in the hippocampus were observed by Nissl staining, and BDNF and phosphorylated TrkB detected by Western blot...
July 6, 2018: Biological Research
https://www.readbyqxmd.com/read/29933757/expression-of-concern-to-knockdown-of-zfr-suppresses-cell-proliferation-and-invasion-of-human-pancreatic-cancer
#3
Xiaolan Zhao, Man Chen, Jishan Tan
Concerns have been raised about this article [1] relating to the appropriateness of the use of the shRNA (5'-GCGGAGGGTTTGAAAGAATATCTCGAGATATTCTTTCAAACCCTCCGCTTTTTT-3') as a non-targeting control and similarities in text and formatting with other published articles. This is currently under investigation and appropriate editorial action will be taken once the investigation is concluded. The authors did not respond to our correspondence regarding this expression of concern.
June 22, 2018: Biological Research
https://www.readbyqxmd.com/read/29933754/expression-of-concern-to-lentivirus-mediated-silencing-of-ubiquitin-specific-peptidase-39-inhibits-cell-proliferation-of-human-hepatocellular-carcinoma-cells-in-vitro
#4
Zeya Pan, Hao Pan, Jin Zhang, Yun Yang, Hui Liu, Yuan Yang, Gang Huang, Junsheng Ni, Jian Huang, Weiping Zhou
Concerns have been raised about this article [1] relating to the appropriateness of the use of the shRNA (5'-GCGGAGGGTTTGAAAGAATATCTCGAGATATTCTTTCAAACCCTCCGCTTTTTT-3') as a non-targeting control and similarities in text and formatting with other published articles. This is currently under investigation and appropriate editorial action will be taken once the investigation is concluded. The authors agree.
June 22, 2018: Biological Research
https://www.readbyqxmd.com/read/29898774/arsenic-trioxide-induces-cell-cycle-arrest-and-affects-trk-receptor-expression-in-human-neuroblastoma-sk-n-sh-cells
#5
Xilin Xiong, Yang Li, Ling Liu, Kai Qi, Chi Zhang, Yueqin Chen, Jianpei Fang
BACKGROUND: Arsenic trioxide (As2 O3 ), a drug that has been used in China for approximately two thousand years, induces cell death in a variety of cancer cell types, including neuroblastoma (NB). The tyrosine kinase receptor (Trk) family comprises three members, namely TrkA, TrkB and TrkC. Various studies have confirmed that TrkA and TrkC expression is associated with a good prognosis in NB, while TrkB overexpression can lead to tumor cell growth and invasive metastasis. Previous studies have shown that As2 O3 can inhibit the growth and proliferation of a human NB cell line and can also affect the N-Myc mRNA expression...
June 13, 2018: Biological Research
https://www.readbyqxmd.com/read/29891016/melatonin-ameliorates-h-2-o-2-induced-oxidative-stress-through-modulation-of-erk-akt-nfkb-pathway
#6
Mahammed Moniruzzaman, Indranath Ghosal, Debjit Das, Suman Bhusan Chakraborty
BACKGROUND: Improper control on reactive oxygen species (ROS) elimination process and formation of free radicals causes tissue dysfunction. Pineal hormone melatonin is considered a potent regulator of such oxidative damage in different vertebrates. Aim of the current communication is to evaluate the levels of oxidative stress and ROS induced damage, and amelioration of oxidative status through melatonin induced activation of signaling pathways. Hepatocytes were isolated from adult Labeo rohita and exposed to H2 O2 at three different doses (12...
June 11, 2018: Biological Research
https://www.readbyqxmd.com/read/29880026/the-effect-of-downregulation-of-stathmin-gene-on-biological-behaviors-of-u373-and-u87-mg-glioblastoma-cells
#7
Ping Liu, Junyan Yu, Xiangyang Tian, Jianlan Chang, Ying Zhang, Rong Zhang, Ningning Zhang, Ranxing Huang, Lulu Li, Xianli Qiao, Hongliang Guo
BACKGROUND: Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. However, extremely little is known about Stathmin in glioblastoma. So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of glioblastoma cells. METHOD: The lentiviral interference vector pLV3-si-Stathmin targeting Stathmin gene and the control vector pLV3-NC were established for the co-transfection of 293T cells together with the helper plasmids...
June 8, 2018: Biological Research
https://www.readbyqxmd.com/read/29866159/extracellular-matrix-and-fibroblast-injection-produces-pterygium-like-lesion-in-rabbits
#8
Judith Zavala, Julio C Hernandez-Camarena, Brenda Salvador-Gálvez, José E Pérez-Saucedo, Amin Vela-Martinez, Jorge E Valdez-García
BACKGROUND: Translational research to develop pharmaceutical and surgical treatments for pterygium requires a reliable and easy to produce animal model. Extracellular matrix and fibroblast are important components of pterygium. The aim of this study was to analyze the effect of the subconjunctival injection of fibroblast cells (NIH3T3 cell line) and exogenous extracellular matrix in rabbits in producing a pterygium-like lesion. METHODS: Six 3-month-old white New Zealand rabbits were injected with 20,000 NIH3T3 cells and 5 µL of Matrigel in the right conjunctiva, and with only 5 µL of Matrigel in the left conjunctiva...
June 5, 2018: Biological Research
https://www.readbyqxmd.com/read/29843798/the-nk1-receptor-antagonist-nkp608-inhibits-proliferation-of-human-colorectal-cancer-cells-via-wnt-signaling-pathway
#9
Xiao-Ling Niu, Jian-Feng Hou, Jing-Xiang Li
BACKGROUND: Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism. METHODS: The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry...
May 30, 2018: Biological Research
https://www.readbyqxmd.com/read/29793538/microrna-1247-inhibits-cell-proliferation-by-directly-targeting-znf346-in-childhood-neuroblastoma
#10
Tingting Wu, Yun Lin, Zhongguo Xie
BACKGROUND: Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. METHODS/RESULTS: We found miR-1247 was downregulated in NB tissues and cells using quantitative PCR analysis. Gain- and loss-of-function studies demonstrated that miR-1247 significantly suppressed cell proliferation and induced cell cycle G0/G1 phase arrest and cell apoptosis of NB cells in vitro by using MTT, colony formation assay and Flow cytometry analysis...
May 24, 2018: Biological Research
https://www.readbyqxmd.com/read/29788995/in-vitro-chemokine-c-c-motif-receptor-6-dependent-non-inflammatory-chemotaxis-during-spermatogenesis
#11
Ruiying Diao, Xueyong Cai, Lu Liu, Lihua Yang, YongGang Duan, Zhiming Cai, Yaoting Gui, Lisha Mou
BACKGROUND: Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis. METHODS AND RESULTS: CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis...
May 22, 2018: Biological Research
https://www.readbyqxmd.com/read/29661232/cmpd1-inhibited-human-gastric-cancer-cell-proliferation-by-inducing-apoptosis-and-g2-m-cell-cycle-arrest
#12
Yu Li, Depeng Zhang, Kaikai Yu, Yudong Hu, Qiong Wu, Feng Qian, Zishu Wang
BACKGROUND: Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. Clinical therapies of gastric cancer remain limited because of uncertainty of mechanisms and shortness of effective medicine. Thus, new drug candidates for gastric cancer treatment is urgently needed. RESULTS: In this study, CMPD1 as a wildly used MK2 phosphorylation inhibitor was employed to find its impact on gastric cancer cell proliferation, apoptosis and cell cycle using colony formation assay and flow cytometry analysis...
April 16, 2018: Biological Research
https://www.readbyqxmd.com/read/29653552/independent-prognostic-genes-and-mechanism-investigation-for-colon-cancer
#13
Chunsheng Li, Zhen Shen, Yangyang Zhou, Wei Yu
PROPOSE: We aimed to explore the potential molecular mechanism and independent prognostic genes for colon cancer (CC). METHODS: Microarray datasets GSE17536 and GSE39582 were downloaded from Gene Expression Omnibus. Meanwhile, the whole CC-related dataset were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed mRNA (DEMs) were identified between cancer tissue samples and para-carcinoma tissue samples in TCGA dataset, followed by the KEGG pathway and GO function analyses...
April 13, 2018: Biological Research
https://www.readbyqxmd.com/read/29604956/berberine-ameliorates-diabetic-nephropathy-by-inhibiting-tlr4-nf-%C3%AE%C2%BAb-pathway
#14
Liping Zhu, Jiakai Han, Rongrong Yuan, Lei Xue, Wuyan Pang
BACKGROUND: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure, contributing to severe morbidity and mortality in diabetic patients. Berberine (BBR) has been well characterized to exert renoprotective effects in DN progression. However, the action mechanism of BBR in DN remains to be fully understood. METHODS: The DN rat model was generated by intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight) while 30 mM high glucose (HG)-treated podocytes were used as an in vitro DN model...
March 31, 2018: Biological Research
https://www.readbyqxmd.com/read/29587857/hsp70-from-the-antarctic-sea-urchin-sterechinus-neumayeri-molecular-characterization-and-expression-in-response-to-heat-stress
#15
Marcelo González-Aravena, Camila Calfio, Luis Mercado, Byron Morales-Lange, Jorn Bethke, Julien De Lorgeril, César A Cárdenas
BACKGROUND: Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequence and the expression of Hsp70 in the Antarctic sea urchin are unknown. METHODS: We amplified and cloned a transcript sequence of 1991 bp from the Antarctic sea urchin Sterechinus neumayeri, experimentally exposed to heat stress (5  and 10 °C for 1, 24 and 48 h)...
March 27, 2018: Biological Research
https://www.readbyqxmd.com/read/29544529/transcriptome-analysis-of-phosphorus-stress-responsiveness-in-the-seedlings-of-dongxiang-wild-rice-oryza-rufipogon-griff
#16
Qian-Wen Deng, Xiang-Dong Luo, Ya-Ling Chen, Yi Zhou, Fan-Tao Zhang, Biao-Lin Hu, Jian-Kun Xie
BACKGROUND: Low phosphorus availability is a major factor restricting rice growth. Dongxiang wild rice (Oryza rufipogon Griff.) has many useful genes lacking in cultivated rice, including stress resistance to phosphorus deficiency, cold, salt and drought, which is considered to be a precious germplasm resource for rice breeding. However, the molecular mechanism of regulation of phosphorus deficiency tolerance is not clear. RESULTS: In this study, cDNA libraries were constructed from the leaf and root tissues of phosphorus stressed and untreated Dongxiang wild rice seedlings, and transcriptome sequencing was performed with the goal of elucidating the molecular mechanisms involved in phosphorus stress response...
March 15, 2018: Biological Research
https://www.readbyqxmd.com/read/29482665/study-on-the-relationship-between-expression-patterns-of-cocaine-and-amphetamine-regulated-transcript-and-hormones-secretion-in-porcine-ovarian-follicles
#17
Pengfei Li, Jinzhu Meng, Jiongjie Jing, Qingling Hao, Zhiwei Zhu, Jianbo Yao, Lihua Lyu
BACKGROUND: Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Further research found that secretion of E2 was inhibited in granulosa cells with a certain dose of CART, the effect depends on the stage of cell differentiation, suggesting that CART could play a crucial role in regulating follicle atresia...
February 26, 2018: Biological Research
https://www.readbyqxmd.com/read/29448959/impaired-t-cell-mediated-hepatitis-in-peroxisome-proliferator-activated-receptor-alpha-ppar%C3%AE-deficient-mice
#18
Ian N Hines, Michael Kremer, Sherri M Moore, Michael D Wheeler
BACKGROUND: Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). METHODS: Wild type (wt) or PPARα-deficient (PPARα-/- ) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization...
February 15, 2018: Biological Research
https://www.readbyqxmd.com/read/29338771/regulation-of-aquaporins-in-plants-under-stress
#19
REVIEW
Ranganathan Kapilan, Maryam Vaziri, Janusz J Zwiazek
Aquaporins (AQP) are channel proteins belonging to the Major Intrinsic Protein (MIP) superfamily that play an important role in plant water relations. The main role of aquaporins in plants is transport of water and other small neutral molecules across cellular biological membranes. AQPs have remarkable features to provide an efficient and often, specific water flow and enable them to transport water into and out of the cells along the water potential gradient. Plant AQPs are classified into five main subfamilies including the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26 like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and X intrinsic proteins (XIPs)...
January 16, 2018: Biological Research
https://www.readbyqxmd.com/read/29316982/the-tankyrase-inhibitor-g007-lk-inhibits-small-intestine-lgr5-stem-cell-proliferation-without-altering-tissue-morphology
#20
Jens Henrik Norum, Ellen Skarpen, Andreas Brech, Raoul Kuiper, Jo Waaler, Stefan Krauss, Therese Sørlie
BACKGROUND: The WNT pathway regulates intestinal stem cells and is frequently disrupted in intestinal adenomas. The pathway contains several potential biotargets for interference, including the poly-ADP ribosyltransferase enzymes tankyrase1 and 2. LGR5 is a known WNT pathway target gene and marker of intestinal stem cells. The LGR5+ stem cells are located in the crypt base and capable of regenerating all intestinal epithelial cell lineages. RESULTS: We treated Lgr5-EGFP-Ires-CreERT2;R26R-Confetti mice with the tankyrase inhibitor G007-LK for up to 3 weeks to assess the effect on duodenal stem cell homeostasis and on the integrity of intestinal epithelium...
January 9, 2018: Biological Research
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