journal
MENU ▼
Read by QxMD icon Read
search

Biological Research

journal
https://www.readbyqxmd.com/read/28629444/microrna-98-inhibits-the-cell-proliferation-of-human-hypertrophic-scar-fibroblasts-via-targeting-col1a1
#1
Sheng Bi, Linlin Chai, Xi Yuan, Chuan Cao, Shirong Li
BACKGROUND: Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS. METHODS: MicroRNA-98 was selected as the key miRNA comprised in HS. The mRNA levels of miR-98 in HS tissues and the matched normal skin tissues were determined by qRT-PCR...
June 19, 2017: Biological Research
https://www.readbyqxmd.com/read/28601089/rapid-culture-based-detection-of-living-mycobacteria-using-microchannel-electrical-impedance-spectroscopy-m-eis
#2
Roli Kargupta, Sachidevi Puttaswamy, Aiden J Lee, Timothy E Butler, Zhongyu Li, Sounak Chakraborty, Shramik Sengupta
BACKGROUND: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection" (TTD). Hence, a method that retains the reliability and low-cost of the MGIT system, while reducing TTD would be highly desirable. METHODS: Living bacterial cells possess a membrane potential, on account of which they store charge when subjected to an AC-field...
June 10, 2017: Biological Research
https://www.readbyqxmd.com/read/28578707/meristem-culture-and-subsequent-micropropagation-of-chilean-strawberry-fragaria-chiloensis-l-duch
#3
Karla A Quiroz, Miguel Berríos, Basilio Carrasco, Jorge B Retamales, Peter D S Caligari, Rolando García-Gonzáles
BACKGROUND: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality...
June 2, 2017: Biological Research
https://www.readbyqxmd.com/read/28545522/brd4-inhibition-suppresses-cell-growth-migration-and-invasion-of-salivary-adenoid-cystic-carcinoma
#4
Limei Wang, Xiuyin Wu, Ruolin Wang, Chengzhe Yang, Zhi Li, Cunwei Wang, Fenghe Zhang, Pishan Yang
BACKGROUND: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). METHODS: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation...
May 25, 2017: Biological Research
https://www.readbyqxmd.com/read/28532517/expression-of-cocaine-and-amphetamine-regulated-transcript-cart-in-hen-ovary
#5
Pengfei Li, Xuejing Yu, Jianshan Xie, Xiaolei Yao, Wenzhong Liu, Jianbo Yao, Zhiwei Zhu, Lihua Lyu
BACKGROUND: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown...
May 22, 2017: Biological Research
https://www.readbyqxmd.com/read/28532516/properties-of-realgar-bioleaching-using-an-extremely-acidophilic-bacterium-and-its-antitumor-mechanism-as-an-anticancer-agent
#6
REVIEW
Peng Chen, Ruixiang Xu, Lei Yan, Zhengrong Wu, Yan Wei, Wenbin Zhao, Xin Wang, Qinjian Xie, Hongyu Li
Realgar is a naturally occurring arsenic sulfide (or Xionghuang, in Chinese). It contains over 90% tetra-arsenic tetra-sulfide (As4S4). Currently, realgar has been confirmed the antitumor activities, both in vitro and in vivo, of realgar extracted using Acidithiobacillus ferrooxidans (A. ferrooxidans). Bioleaching, a new technology to greatly improve the use rate of arsenic extraction from realgar using bacteria, is a novel methodology that addressed a limitation of the traditional method for realgar preparation...
May 22, 2017: Biological Research
https://www.readbyqxmd.com/read/28526061/photosynthesis-at-the-far-red-region-of-the-spectrum-in-acaryochloris-marina
#7
REVIEW
Syed Lal Badshah, Yahia Mabkhot, Salim S Al-Showiman
Acaryochloris marina is an oxygenic cyanobacterium that utilizes far-red light for photosynthesis. It has an expanded genome, which helps in its adaptability to the environment, where it can survive on low energy photons. Its major light absorbing pigment is chlorophyll d and it has α-carotene as a major carotenoid. Light harvesting antenna includes the external phycobilin binding proteins, which are hexameric rods made of phycocyanin and allophycocyanins, while the small integral membrane bound chlorophyll binding proteins are also present...
May 19, 2017: Biological Research
https://www.readbyqxmd.com/read/28403900/knockdown-of-ubiquitin-specific-peptidase-39-inhibited-the-growth-of-osteosarcoma-cells-and-induced-apoptosis-in-vitro
#8
Zhihua Gan, Kun Han, Shuchen Lin, Haiyan Hu, Zan Shen, Daliu Min
BACKGROUND: Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. METHODS: The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database...
April 12, 2017: Biological Research
https://www.readbyqxmd.com/read/28376862/morphological-molecular-and-ftir-spectroscopic-analysis-during-the-differentiation-of-kidney-cells-from-pluripotent-stem-cells
#9
Monica Maribel Mata-Miranda, Gustavo Jesus Vazquez-Zapien, Marlon Rojas-Lopez, Virginia Sanchez-Monroy, David Guillermo Perez-Ishiwara, Raul Jacobo Delgado-Macuil
BACKGROUND: Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. RESULTS: In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs...
April 4, 2017: Biological Research
https://www.readbyqxmd.com/read/28347317/erratum-to-metabotropic-glutamate-receptor-5-may-be-involved-in-macrophage-plasticity
#10
Lali Shanshiashvili, Elene Tsitsilashvili, Nino Dabrundashvili, Irine Kalandadze, David Mikeladze
No abstract text is available yet for this article.
March 27, 2017: Biological Research
https://www.readbyqxmd.com/read/28302167/proton-nmr-characterization-of-intact-primary-and-metastatic-melanoma-cells-in-2d-3d-cultures
#11
Gokula Krishnan Ramachandran, Chen Hua Yeow
OBJECTIVE: To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures. METHODS: Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed. RESULTS: In monolayer culture (2D), we observed one qualitative chemical shift marker for primary melanoma cells...
March 16, 2017: Biological Research
https://www.readbyqxmd.com/read/28288692/does-cimicifuga-racemosa-have-the-effects-like-estrogen-on-the-sublingual-gland-in-ovariectomized-rats
#12
Yun-Meng Da, Kai-Yu Niu, Shu-Ya Liu, Ke Wang, Wen-Juan Wang, Jing Jia, Li-Hua Qin, Wen-Pei Bai
BACKGROUND: Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been reported whether C. racemosa has any impact on the sublingual gland, which may relate to dry mouth. In an attempt to determine this, we have compared the effects of estrogen and C. racemosa on the sublingual gland of ovariectomized rats...
March 14, 2017: Biological Research
https://www.readbyqxmd.com/read/28259185/musk-gland-seasonal-development-and-musk-secretion-are-regulated-by-the-testis-in-muskrat-ondatra-zibethicus
#13
Tianxiang Zhang, Dong Peng, Lei Qi, Weixuan Li, Mengyuan Fan, Jiachen Shen, Liangliang Yang, Yihua Wang, Wenxia Wang, Xiaolong Hu, Ruibo Cai, Ran Zhou, Yuting Wei, Juntong Zhou, Shuang Yang, Defu Hu, Shuqiang Liu
BACKGROUND: The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process. METHODS: The musk gland, testis and blood samples of musk rats are collected in both breeding and non-breeding seasons. Some part of the samples are kept in liquid nitrogen for transcriptome analysis and Western blotting test...
March 4, 2017: Biological Research
https://www.readbyqxmd.com/read/28249617/alpinia-oxyphylla-miq-extract-changes-mirna-expression-profiles-in-db-db-mouse-kidney
#14
Guankui Du, Man Xiao, Xuezi Zhang, Maoyu Wen, Chi Pang, Shangfei Jiang, Shenggang Sang, Yiqiang Xie
BACKGROUND: A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-). RESULTS: Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine...
March 1, 2017: Biological Research
https://www.readbyqxmd.com/read/28241881/bj-1108-a-6-amino-2-4-5-trimethylpyridin-3-ol-analogue-regulates-differentiation-of-th1-and-th17-cells-to-ameliorate-experimental-autoimmune-encephalomyelitis
#15
Youra Kang, Maheshwor Timilshina, Tae-Gyu Nam, Byeong-Seon Jeong, Jae-Hoon Chang
BACKGROUND: CD4(+) T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-γ (IFN-γ)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases...
February 28, 2017: Biological Research
https://www.readbyqxmd.com/read/28241869/de-novo-transcriptome-assembly-functional-annotation-and-differential-gene-expression-analysis-of-juvenile-and-adult-e-fetida-a-model-oligochaete-used-in-ecotoxicological-studies
#16
Michelle Thunders, Jo Cavanagh, Yinsheng Li
BACKGROUND: Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. RESULTS: This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool...
February 27, 2017: Biological Research
https://www.readbyqxmd.com/read/28231844/analysis-of-gene-expression-changes-associated-with-human-carcinoma-associated-fibroblasts-in-non-small-cell-lung-carcinoma
#17
Xiaofen Wu, Lei Ruan, Yi Yang, Qi Mei
BACKGROUND: This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC). METHODS: We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R...
February 23, 2017: Biological Research
https://www.readbyqxmd.com/read/28202086/differential-roles-for-pathogenicity-islands-spi-13-and-spi-8-in-the-interaction-of-salmonella-enteritidis-and-salmonella-typhi-with-murine-and-human-macrophages
#18
Rodrigo A Espinoza, Cecilia A Silva-Valenzuela, Fernando A Amaya, Ítalo M Urrutia, Inés Contreras, Carlos A Santiviago
BACKGROUND: Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264...
February 15, 2017: Biological Research
https://www.readbyqxmd.com/read/28196513/metabotropic-glutamate-receptor-5-may-be-involved-in-macrophage-plasticity
#19
Lali Shanshiashvili, Elene Tsitsilashvili, Nino Dabrundashvili, Irine Kalandadze, David Mikeladze
BACKGROUND: Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264.7 cells. RESULTS: Comparative analysis of modified (RAW-mGluR5 macrophages) and non-modified macrophages (RAW-macrophages) has shown that the RAW-mGluR5 macrophages absorbed more glutamate than control cells and the amount of intracellular glutamate correlated with the expression of excitatory amino acid transporters -2 (EAAT-2)...
February 14, 2017: Biological Research
https://www.readbyqxmd.com/read/28193284/selective-intra-dinucleotide-interactions-and-periodicities-of-bases-separated-by-k-sites-a-new-vision-and-tool-for-phylogeny-analyses
#20
REVIEW
Carlos Y Valenzuela
Direct tests of the random or non-random distribution of nucleotides on genomes have been devised to test the hypothesis of neutral, nearly-neutral or selective evolution. These tests are based on the direct base distribution and are independent of the functional (coding or non-coding) or structural (repeated or unique sequences) properties of the DNA. The first approach described the longitudinal distribution of bases in tandem repeats under the Bose-Einstein statistics. A huge deviation from randomness was found...
February 13, 2017: Biological Research
journal
journal
31356
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"