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Biological Research

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https://www.readbyqxmd.com/read/29448959/impaired-t-cell-mediated-hepatitis-in-peroxisome-proliferator-activated-receptor-alpha-ppar%C3%AE-deficient-mice
#1
Ian N Hines, Michael Kremer, Sherri M Moore, Michael D Wheeler
BACKGROUND: Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). METHODS: Wild type (wt) or PPARα-deficient (PPARα -/- ) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization...
February 15, 2018: Biological Research
https://www.readbyqxmd.com/read/29338771/regulation-of-aquaporins-in-plants-under-stress
#2
REVIEW
Ranganathan Kapilan, Maryam Vaziri, Janusz J Zwiazek
Aquaporins (AQP) are channel proteins belonging to the Major Intrinsic Protein (MIP) superfamily that play an important role in plant water relations. The main role of aquaporins in plants is transport of water and other small neutral molecules across cellular biological membranes. AQPs have remarkable features to provide an efficient and often, specific water flow and enable them to transport water into and out of the cells along the water potential gradient. Plant AQPs are classified into five main subfamilies including the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26 like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and X intrinsic proteins (XIPs)...
January 16, 2018: Biological Research
https://www.readbyqxmd.com/read/29316982/the-tankyrase-inhibitor-g007-lk-inhibits-small-intestine-lgr5-stem-cell-proliferation-without-altering-tissue-morphology
#3
Jens Henrik Norum, Ellen Skarpen, Andreas Brech, Raoul Kuiper, Jo Waaler, Stefan Krauss, Therese Sørlie
BACKGROUND: The WNT pathway regulates intestinal stem cells and is frequently disrupted in intestinal adenomas. The pathway contains several potential biotargets for interference, including the poly-ADP ribosyltransferase enzymes tankyrase1 and 2. LGR5 is a known WNT pathway target gene and marker of intestinal stem cells. The LGR5+ stem cells are located in the crypt base and capable of regenerating all intestinal epithelial cell lineages. RESULTS: We treated Lgr5-EGFP-Ires-CreERT2;R26R-Confetti mice with the tankyrase inhibitor G007-LK for up to 3 weeks to assess the effect on duodenal stem cell homeostasis and on the integrity of intestinal epithelium...
January 9, 2018: Biological Research
https://www.readbyqxmd.com/read/29316975/inducible-t-cell-co-stimulators-regulate-the-proliferation-and-invasion-of-human-hepatocellular-carcinoma-hepg2-cells
#4
Yaning Wei, Yanan Wang, Aimin Zang, Yanhong Shang, Zizheng Song, Zhiyu Wang, Yangyang Wang, Hua Yang
BACKGROUND: This study determined the regulatory effects of inducible T-cell co-stimulators (ICOS) in human hepatocellular carcinoma HepG2 cells using a RNA interference (RNAi) technique. METHODS: A RNAi technique was used to knockdown the expression of ICOS. ICOS expression after knockdown was detected as mRNA and protein levels by RT-PCR and Western blot, respectively. A MTT colorimetric assay was used to detect cell proliferation, and the Transwell assay was used to detect cell invasion...
January 9, 2018: Biological Research
https://www.readbyqxmd.com/read/29298720/ccat2-is-an-oncogenic-long-non-coding-rna-in-pancreatic-ductal-adenocarcinoma
#5
Yi Cai, Xiaomei Li, Peng Shen, Dong Zhang
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with poor prognosis. Long non-coding RNAs (lncRNAs), a group of non-coding RNAs, play important roles in the progression of PDAC. This study aimed to investigate the potential involvement of lncRNA CCAT2 in PDAC tumorigenesis. METHODS: Expression of CCAT2 was detected by quantitative real-time PCR (qRT-PCR) in 80 human PDAC tissues and three PDAC cell lines. The effects of CCAT2 silencing in PANC-1 cells on cell proliferation and invasion were studied using MTT assay and transwell assay, respectively...
January 3, 2018: Biological Research
https://www.readbyqxmd.com/read/29279050/expression-regulation-and-functional-analysis-of-rgs2-and-rgs4-in-adipogenic-and-osteogenic-differentiation-of-human-mesenchymal-stem-cells
#6
Alma Madrigal, Lun Tan, Yuanxiang Zhao
BACKGROUND: Understanding the molecular basis underlying the formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into the cause of disorders originating in stem/progenitor cells and develop therapeutic treatments for bone- or adipose-related diseases. In this study, the role of RGS2 and RGS4, two members of the regulators of G protein signaling (RGS) family, was investigated during adipogenenic and osteogenenic differentiation of human mesenchymal stem cells (hMSCs)...
December 26, 2017: Biological Research
https://www.readbyqxmd.com/read/29268779/effect-of-fresh-frozen-plasma-on-the-in-vitro-activation-of-u937-monocytes-a-potential-role-for-the-age-of-blood-donors-and-their-underlying-cytokine-profile
#7
Mariana Patlán, Fausto Sánchez-Muñoz, Luis M Amezcua-Guerra, Adriana Granados, Araceli Páez, Felipe Massó, Ana M Mejía, Angeles Soster, Rafael Bojalil, Lenin Pavón, Luis A Jiménez-Zamudio, Ricardo Márquez-Velasco
BACKGROUND: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors. METHODS: FFP donors were stratified into groups of 19-30 years, 31-40 years or 41-50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide-LPS) for 24 h...
December 21, 2017: Biological Research
https://www.readbyqxmd.com/read/29233192/methanolic-extract-of-euchelus-asper-exhibits-in-ovo-anti-angiogenic-and-in-vitro-anti-proliferative-activities
#8
Sweta Agrawal, Sachin Chaugule, Shashank More, Gargi Rane, Madhavi Indap
BACKGROUND: The marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. The present study was focused on evaluating the anti-angiogenic and anti- proliferative activities of methanolic extract of Euchelus asper (EAME). METHODS: The anti-angiogenic activity of EAME (50-800 μg/mL) was assessed by chick chorio-allantoic membrane (CAM) model wherein multiple parameters in the CAM blood vessels were analysed through morphometric and histological investigations...
December 12, 2017: Biological Research
https://www.readbyqxmd.com/read/29228993/programmed-cell-death-5-transgenic-mice-attenuates-adjuvant-induced-arthritis-by-2-modifying-the-t-lymphocytes-balance
#9
Feng Yuan, Junfeng Wang, Keshi Zhang, Zhao Li, Zhenpeng Guan
BACKGROUND: Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice. RESULTS: In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice...
December 11, 2017: Biological Research
https://www.readbyqxmd.com/read/29221464/molecular-and-functional-characterization-of-ferredoxin-nadp-h-oxidoreductase-from-gracilaria-chilensis-and-its-complex-with-ferredoxin
#10
María Alejandra Vorphal, Carola Bruna, Traudy Wandersleben, Jorge Dagnino-Leone, Francisco Lobos-González, Elena Uribe, José Martínez-Oyanedel, Marta Bunster
BACKGROUD: Ferredoxin NADP(H) oxidoreductases (EC 1.18.1.2) (FNR) are flavoenzymes present in photosynthetic organisms; they are relevant for the production of reduced donors to redox reactions, i.e. in photosynthesis, the reduction of NADP+ to NADPH using the electrons provided by Ferredoxin (Fd), a small FeS soluble protein acceptor of electrons from PSI in chloroplasts. In rhodophyta no information about this system has been reported, this work is a contribution to the molecular and functional characterization of FNR from Gracilaria chilensis, also providing a structural analysis of the complex FNR/Fd...
December 8, 2017: Biological Research
https://www.readbyqxmd.com/read/29169375/synaptic-configuration-of-quadrivalents-and-their-association-with-the-xy-bivalent-in-spermatocytes-of-robertsonian-heterozygotes-of-mus-domesticus
#11
Soledad Berríos, Raúl Fernández-Donoso, Eliana Ayarza
BACKGROUND: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains and consequently is prone to modification by chromosomal rearrangements. We have shown that nuclear architecture is modified in spermatocytes of Robertsonian (Rb) homozygotes of Mus domesticus. In this study we analyse the synaptic configuration of the quadrivalents formed in the meiotic prophase of spermatocytes of mice double heterozygotes for the dependent Rb chromosomes: Rbs 11...
November 23, 2017: Biological Research
https://www.readbyqxmd.com/read/29162157/role-of-regenerating-gene-ia-expression-on-local-invasion-and-survival-in-nasopharyngeal-carcinoma
#12
Haijie Xing, Xiangdong Chen, Yaofeng Han
BACKGROUND: Regenerating gene IA (REGIA) plays an important role in tissue regeneration and tumors prognosis of epithelium origin. However, the role of REGIA in nasopharyngeal carcinoma (NPC) is unclear. This study aims to investigate the expression and function of REG1A in NPC. RESULTS: We have found that there was 63 patients with REGIA positive expression of 155 patients in this study (40.65%). The positive expression rate of REGIA was 30.50, 44.44 and 47.83% in stage T2, T3 and T4 patients, respectively...
November 21, 2017: Biological Research
https://www.readbyqxmd.com/read/29041990/piceatannol-induced-apoptosis-through-up-regulation-of-microrna-181a-in-melanoma-cells
#13
Maotao Du, Zhong Zhang, Tao Gao
BACKGROUND: Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells...
October 17, 2017: Biological Research
https://www.readbyqxmd.com/read/28985766/mutations-in-brca1-brca2-and-other-breast-and-ovarian-cancer-susceptibility-genes-in-central-and-south-american-populations
#14
REVIEW
Lilian Jara, Sebastian Morales, Tomas de Mayo, Patricio Gonzalez-Hormazabal, Valentina Carrasco, Raul Godoy
Breast cancer (BC) is the most common malignancy among women worldwide. A major advance in the understanding of the genetic etiology of BC was the discovery of BRCA1 and BRCA2 (BRCA1/2) genes, which are considered high-penetrance BC genes. In non-carriers of BRCA1/2 mutations, disease susceptibility may be explained of a small number of mutations in BRCA1/2 and a much higher proportion of mutations in ethnicity-specific moderate- and/or low-penetrance genes. In Central and South American populations, studied have focused on analyzing the distribution and prevalence of BRCA1/2 mutations and other susceptibility genes that are scarce in Latin America as compared to North America, Europe, Australia, and Israel...
October 6, 2017: Biological Research
https://www.readbyqxmd.com/read/28982393/the-comparative-mitogenomics-and-phylogenetics-of-the-two-grouse-grasshoppers-insecta-orthoptera-tetrigoidea
#15
Yufang Sun, Dianfeng Liu, Bo Xiao, Guofang Jiang
OBJECTIVE: This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species. METHODS: The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively...
October 5, 2017: Biological Research
https://www.readbyqxmd.com/read/28969709/awareness-and-current-knowledge-of-breast-cancer
#16
REVIEW
Muhammad Akram, Mehwish Iqbal, Muhammad Daniyal, Asmat Ullah Khan
Breast cancer remains a worldwide public health dilemma and is currently the most common tumour in the globe. Awareness of breast cancer, public attentiveness, and advancement in breast imaging has made a positive impact on recognition and screening of breast cancer. Breast cancer is life-threatening disease in females and the leading cause of mortality among women population. For the previous two decades, studies related to the breast cancer has guided to astonishing advancement in our understanding of the breast cancer, resulting in further proficient treatments...
October 2, 2017: Biological Research
https://www.readbyqxmd.com/read/28969673/upregulation-of-lncrna-bancr-associated-with-the-lymph-node-metastasis-and-poor-prognosis-in-colorectal-cancer
#17
Xiaogang Shen, Yifeng Bai, Bin Luo, Xiaogang Zhou
BACKGROUND: Growing evidence has supported that long non-coding RNAs (lncRNAs) could play vital roles in the development, progression, and prognosis of colorectal cancer (CRC). However, little is known about the clinical significance of BRAF-activated non-coding RNA (BANCR) in CRC. The aim of this study is to explore the clinical value of lncRNA BANCR in CRC patients. METHODS: The expression of lncRNA BANCR was measured in 106 CRC tissues and 65 adjacent normal tissues using the quantitative real-time PCR...
October 2, 2017: Biological Research
https://www.readbyqxmd.com/read/28934984/a-simple-and-rapid-identification-method-for-newly-emerged-porcine-deltacoronavirus-with-loop-mediated-isothermal-amplification
#18
Fanfan Zhang, Yu Ye, Deping Song, Nannan Guo, Qi Peng, Anqi Li, Xingrong Zhou, Yanjun Chen, Min Zhang, Dongyan Huang, Yuxin Tang
BACKGROUND: Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. RESULTS: In this study, we developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay specific for nucleocapsid gene to diagnose and monitor PDCoV infections...
September 21, 2017: Biological Research
https://www.readbyqxmd.com/read/28934982/mir-320-inhibits-the-growth-of-glioma-cells-through-downregulating-pbx3
#19
Cuicui Pan, Hua Gao, Ni Zheng, Qi Gao, Yuanquan Si, Yueran Zhao
BACKGROUND: MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. METHODS: Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays...
September 21, 2017: Biological Research
https://www.readbyqxmd.com/read/28927446/neurochemical-and-behavioral-characterization-of-neuronal-glutamate-transporter-eaat3-heterozygous-mice
#20
Luis F González, Francisca Henríquez-Belmar, Claudia Delgado-Acevedo, Marisol Cisternas-Olmedo, Gloria Arriagada, Ramón Sotomayor-Zárate, Dennis L Murphy, Pablo R Moya
BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine...
September 19, 2017: Biological Research
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