journal
MENU ▼
Read by QxMD icon Read
search

Journal of Eukaryotic Microbiology

journal
https://www.readbyqxmd.com/read/28618210/the-non-canonical-substrates-of-trypanosoma-cruzi-tyrosine-and-aspartate-aminotransferases-branched-chain-amino-acids
#1
N C Manchola, A M Silber, C Nowicki
Trypanosoma cruzi, the etiological agent of Chagas disease, lacks genes that encode canonical branched-chain aminotransferases. However, early studies showed that when epimastigotes were grown in the presence of (14) C1 -DL-leucine, the label was incorporated into various intermediates. More recently, our studies provided evidence that T. cruzi epimastigotes display a single ATP-dependent and saturable transport system that enables epimastigotes to uptake branched-chain amino acids (BCAAs) from the culture media...
June 15, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28605078/morphology-and-phylogenetic-analyses-of-three-novel-naegleria-isolated-from-freshwaters-on-jeju-island-korea-during-the-winter-period
#2
Woo Jun Khwon, Jong Soo Park
The genus Naegleria is one of the best known heterolobosean groups, and is the causative agent of primary amoebic meningoencephalitis. This group is rarely studied in temperate regions during winter. Here, three novel Naegleria were isolated from freshwaters on Jeju Island, Korea, during winter. Two isolates were amoeboflagellates, and one of the three amoebae did not undergo enflagellation. All amoebae had eruptive pseudopodia, and the layer of refractile granules around a large nucleus. They formed a cyst with ~2 pores in the cyst stage...
June 12, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28544317/fish-parasite-dinoflagellates-haidadinium-ichthyophilum-and-piscinoodinium-share-a-recent-common-ancestor
#3
Elisabeth Hehenberger, Erick R James, Javier Del Campo, John A Buckland-Nicks, Thomas E Reimchen, Patrick J Keeling
The dinoflagellate Haidadinium ichthyophilum Buckland-Nicks, Reimchen and Garbary 1997 is an ectoparasite of the spine-deficient, three-spine stickleback Gasterosteus aculeatus L. Reimchen 1984, a fish endemic to Rouge Lake, Haida Gwaii. Haidadinium ichthyophilum proved difficult to assign taxonomically, because its morphology and complex life cycle exhibited defining characteristics of both autotrophic and heterotrophic dinoflagellates, and was tentatively assigned to the Phytodiniales. Here, we characterized a 492bp-fragment of the small subunit ribosomal RNA (SSU rRNA) from preserved H...
May 25, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28544006/morphology-and-phylogeny-of-two-novel-ciliates-arcanisutura-chongmingiensis-n-gen-n-sp-and-naxella-paralucida-n-sp-from-shanghai-china
#4
Wanying Liao, Xinpeng Fan, Qianqian Zhang, Yuan Xu, Fukang Gu
The morphologies of two novel ciliates, Arcanisutura chongmingiensis n. gen., n. sp. and Naxella paralucida n. sp., collected from Shanghai, China, have been investigated using live observation and silver staining methods. Arcanisutura n. gen. can be easily distinguished from related genera by its inconspicuous, oblique anterior suture. Arcanisutura chongmingiensis n. sp. is mainly recognized by its elongated body with a tail-like posterior end, 25-33 somatic kineties, and 4-11 excretory pores. Naxella paralucida n...
May 25, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28544395/morphology-and-molecular-phylogeny-of-a-new-hypotrich-ciliate-pseudourostyla-guizhouensis-sp-nov-from-southern-china-with-notes-on-a-chinese-population-of-hemicycliostyla-franzi-foissner-1987-paiva-et%C3%A2-al-2012-ciliophora-hypotricha
#5
REVIEW
Yanbo Li, Zhao Lyu, Alan Warren, Kexin Zhou, Fengchao Li, Xumiao Chen
The morphology and molecular phylogeny of a soil hypotrich ciliate, Pseudourostyla guizhouensis sp. nov., collected from southern China, were investigated. Pseudourostyla guizhouensis sp. nov. has an elongate elliptical body measuring 180-310 × 65-85 μm in vivo; invariably two right and three or four left marginal rows; six or seven dorsal kineties; adoral zone consisting of 57-70 membranelles; 12-16 frontal cirri, one buccal cirrus, 13-20 midventral pairs, two frontoterminal cirri, two pre-transverse ventral cirri, and five to seven transverse cirri...
May 22, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28509379/microbial-eukaryotes-that-lack-sterols
#6
Kiyotaka Takishita, Yoshito Chikaraishi, Goro Tanifuji, Naohiko Ohkouchi, Tetsuo Hashimoto, Katsunori Fujikura, Andrew J Roger
It is widely held that sterols are key cyclic triterpenoid lipids in eukaryotic cell membranes and are synthesized through oxygen-dependent multienzyme pathways. However, there are known exceptions-ciliated protozoans, such as Tetrahymena, along with diverse low-oxygen-adapted eukaryotes produce, instead of sterols, the cyclic triterpenoid lipid tetrahymanol that does not require molecular oxygen for its biosynthesis. Here, we report that a number of anaerobic microbial eukaryotes (protists) utilize neither sterols nor tetrahymanol in their membranes...
May 16, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28508432/exploring-cryptic-diversity-and-distribution-patterns-in-the-mallomonas-kalinae-rasilis-species-complex-with-a-description-of-a-new-taxon-mallomonas-furtiva-sp-nov
#7
Evgeniy S Gusev, Dora Čertnerová, Magda Škaloudová, Pavel Škaloud
A complex of closely related Mallomonas taxa belonging to the section Papillosae, M. kalinae Řezáčová and M. rasilis Dürrschmidt, have been studied in detail by molecular and morphometric methods. Our investigations uncovered the existence of a new species found in water bodies in Vietnam, which we describe here as Mallomonas furtiva sp. nov. This taxon is morphologically very similar to M. kalinae, from which it differs by minute, but statistically significant morphological differences on the structure of silica scales...
May 16, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28499076/single-cell-analysis-linking-ribosomal-r-dna-and-rrna-copy-numbers-to-cell-size-and-growth-rate-provides-insights-into-molecular-protistan-ecology
#8
Rao Fu, Jun Gong
Ribosomal (r)RNA and rDNA have been golden molecular markers in microbial ecology. However, it remains poorly understood how ribotype copy number (CN)-based characteristics are linked with diversity, abundance and activity of protist populations and communities observed at organismal levels. Here, we applied a single-cell approach to quantify ribotype CNs in two ciliate species reared at different temperatures. We found that in actively growing cells, the per-cell rDNA and rRNA CNs scaled with cell volume (CV) to 0...
May 12, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28493395/in-memoriam-michael-levandowsky-1935-2016
#9
Cyrus J Bacchi, Thomas Gorrell, George McManus, Nigel Yarlett
No abstract text is available yet for this article.
May 11, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28477402/tfvps32-regulates-cell-division-in-the-parasite-tritrichomonas-foetus
#10
Lucrecia S Iriarte, Victor Midlej, Lorena S Frontera, Daniel Moros Duarte, Claudio G Barbeito, Wanderley de Souza, Marlene Benchimol, Natalia de Miguel, Veronica M Coceres
The flagellated protist Tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. Cell division has been described as a key player in controlling cell survival in other cells, including parasites but there is no information on the regulation of this process in T. foetus. The regulation of cytokinetic abscission, the final stage of cell division, is mediated by members of the ESCRT (endosomal sorting complex required for transport) machinery. VPS32 is a subunit within the ESCRTIII complex and here, we report that TfVPS32 is localized on cytoplasmic vesicles and a redistribution of the protein to the midbody is observed during the cellular division...
May 6, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28474836/the-ciliary-protein-ift57-in-the-macronucleus-of-paramecium
#11
Lei Shi, France Koll, Olivier Arnaiz, Jean Cohen
The intraflagellar transport IFT57 protein is essential for ciliary growth and maintenance. Also known as HIPPI, human IFT57 can be translocated to the nucleus via a molecular partner of the Huntingtin, Hip1, inducing gene expression changes. In Paramecium tetraurelia, we identified four IFT57 genes forming two subfamilies IFT57A/B and IFT57C/D arising from whole genome duplications. The depletion of proteins of the two subfamilies induced ciliary defects and IFT57A and IFT57C localized in basal bodies and cilia...
May 5, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28460157/nuclear-features-of-the-heterotrich-ciliate-blepharisma-americanum-genomic-amplification-life-cycle-and-nuclear-inclusion
#12
Megan M Wancura, Ying Yan, Laura A Katz, Xyrus X Maurer-Alcalá
Blepharisma americanum, a member of the understudied ciliate class Heterotrichea, has a moniliform somatic macronucleus that resembles beads on a string. Blepharisma americanum is distinguishable by its pink coloration derived from the autofluorescent pigment blepharismin and tends to have a single somatic macronucleus with 3-6 nodes and multiple germline micronuclei. We used fluorescence confocal microscopy to explore the DNA content and amplification between the somatic and germline nuclei of B. americanum through its life cycle...
April 29, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28459502/morphology-and-molecular-phylogeny-of-two-new-ciliates-holostichides-heterotypicus-n-sp-and-holosticha-muuiensis-n-sp-ciliophora-urostylida
#13
Kang-San Kim, Jae-Ho Jung, Gi-Sik Min
Two new urostylid species, Holostichides heterotypicus n. sp. and Holosticha muuiensis n. sp., were discovered in South Korea. Morphological and phylogenetic analyses were carried out to confirm that these species are new to science. Holostichides heterotypicus is mainly characterized by the following combination of features: 110-205 μm long in vivo; 5-10 frontoterminal cirri; 6-8 midventral pairs with 2-3 midventral cirral rows; cortical granules present; four bipolar dorsal kineties; and 6-9 caudal cirri...
April 29, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28444795/systematics-and-molecular-phylogeny-of-the-ciliate-genus-pseudokeronopsis-ciliophora-hypotrichia
#14
Ju Li, Zifeng Zhan, Kuidong Xu
A still challenging study on ciliate systematics is to clarify the taxonomic and phylogenetic confusions of the fascinating pigmented Pseudokeronopsis species because of their high interspecific similarities. We evaluated the identities and internal relationships within Pseudokeronopsis based on morphological descriptions in combination with the 18S and ITS-5.8S rDNA genetic distances, ITS2 secondary structures and phylogenetic analyses. Results showed that (1) Pseudokeronopsis pararubra, a species ever synonymized as Pseudokeronopsis carnea, is a valid species; (2) most Chinese populations identified as Pseudokeronopsis rubra represent a new species namely Pseudokeronopsis songi sp...
April 26, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28432811/yu-et%C3%A2-al-host-cell-mutants-resistant-to-cryptosporidium-parvum-characterization-of-host-cell-mutants-significantly-resistant-to-cryptosporidium-parvum-infection
#15
Xue Yu, Haili Zhang, Guan Zhu
Cryptosporidium parvum is a parasitic protist and a causative agent of mild to severe diarrheal diseases in humans and animals. Despite its globally recognized importance, knowledge on the mechanism of parasite invasion and molecular interactions between host cells and the parasite is limited. Here we report the establishment of 43 mutant cell lines derived from HCT-8 cells by UV-induced mutagenesis and the characterization of three mutants with significantly reduced susceptibility to cryptosporidial infection...
April 22, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28397389/cyst-theca-relationship-and-phylogenetic-position-of-impagidinium-caspienense-incubated-from-caspian-sea-surface-sediments-relation-to-gonyaulax-baltica-and-evidence-for-heterospory-within-gonyaulacoid-dinoflagellates
#16
Kenneth N Mertens, Yoshihito Takano, Haifeng Gu, Siamak Bagheri, Vera Pospelova, Anna J Pieńkowski, Suzanne Leroy, Kazumi Matsuoka
We investigate the cyst-theca relationship of Impagidinium caspienense. Through an incubation experiment, we succeeded in examining the motile stage. Additional molecular analysis of single-cyst PCR (LSU and SSU rDNA) reveal that the cyst is related to the species Gonyaulax baltica Ellegaard et al. (). The ability of this species to belong to two types of cyst-based genera (spiniferate and impagidinioid) suggests that environmental (particularly salinity) and not genetic factors explain the formation of both morphotypes by G...
April 11, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28337822/unieuk-time-to-speak-a-common-language-in-protistology
#17
REVIEW
Cédric Berney, Andreea Ciuprina, Sara Bender, Juliet Brodie, Virginia Edgcomb, Eunsoo Kim, Jeena Rajan, Laura Wegener Parfrey, Sina Adl, Stéphane Audic, David Bass, David A Caron, Guy Cochrane, Lucas Czech, Micah Dunthorn, Stefan Geisen, Frank Oliver Glöckner, Frédéric Mahé, Christian Quast, Jonathan Z Kaye, Alastair G B Simpson, Alexandros Stamatakis, Javier Del Campo, Pelin Yilmaz, Colomban de Vargas
Universal taxonomic frameworks have been critical tools to structure the fields of botany, zoology, mycology, and bacteriology as well as their large research communities. Animals, plants, and fungi have relatively solid, stable morpho-taxonomies built over the last three centuries, while bacteria have been classified for the last three decades under a coherent molecular taxonomic framework. By contrast, no such common language exists for microbial eukaryotes, even though environmental '-omics' surveys suggest that protists make up most of the organismal and genetic complexity of our planet's ecosystems! With the current deluge of eukaryotic meta-omics data, we urgently need to build up a universal eukaryotic taxonomy bridging the protist -omics age to the fragile, centuries-old body of classical knowledge that has effectively linked protist taxa to morphological, physiological, and ecological information...
May 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28370779/comparison-of-recombinant-proteins-of-kinesin-39-heat-shock-protein-70-heat-shock-protein-83-and-glycoprotein-63-for-antibody-detection-of-leishmania-martiniquensis-infection
#18
Suradej Siripattanapipong, Hirotomo Kato, Peerapan Tan-Ariya, Mathirut Mungthin, Saovanee Leelayoova
Leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (CL) and visceral leishmaniasis (VL) among humans and animals. This organism, firstly reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases of L. martiniquensis infection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission...
March 28, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28337817/the-cytotoxic-effects-of-camptothecin-and-mastoparan-on-the-unicellular-green-alga-chlamydomonas-reinhardtii
#19
Jürgen Voigt, Markus Morawski, Johannes Wöstemeyer
We have recently reported that protease inhibitors affecting the activity of the proteasome cause necrotic cell death in Chlamydomonas reinhardtii instead of inducing apoptosis as shown for some mammalian cell lines. Therefore, we have studied other well-known inducers of apoptosis in mammalian cells for their effects on C. reinhardtii cells. Mastoparan caused rapid cell death without a prominent lag-phase under all growth conditions, whereas the cytotoxic effect of the topoisomerase I inhibitor camptothecin exclusively occurred during the cell-division phase...
March 24, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28321967/comparative-analysis-of-the-proteins-with-tandem-repeats-from-8-microsporidia-and-characterization-of-a-novel-endospore-wall-protein-colocalizing-with-polar-tube-from-nosema-bombycis
#20
Ying Wang, Huixia Geng, Xiaoqun Dang, Heng Xiang, Tian Li, Guoqing Pan, Zeyang Zhou
As a common feature of eukaryotic proteins, tandem amino acid repeat has been studied extensively in both animal and plant proteins. Here, a comparative analysis focusing on the proteins having tandem repeats was conducted in eight microsporidia, including four mammal-infecting microsporidia (Encephalitozoon cuniculi, Encephalitozoon intestinalis, Encephalitozoon hellem and Encephalitozoon bieneusi) and four insect-infecting microsporidia (Nosema apis, Nosema ceranae, Vavraia culicis and Nosema bombycis). We found that the proteins with tandem repeats were abundant in these species...
March 21, 2017: Journal of Eukaryotic Microbiology
journal
journal
31323
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"