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Journal of Eukaryotic Microbiology

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https://www.readbyqxmd.com/read/28321967/comparative-analysis-of-the-proteins-with-tandem-repeats-from-8-microsporidia-and-characterization-of-a-novel-endospore-wall-protein-colocalizing-with-polar-tube-from-nosema-bombycis
#1
Ying Wang, Huixia Geng, Xiaoqun Dang, Heng Xiang, Tian Li, Guoqing Pan, Zeyang Zhou
As a common feature of eukaryotic proteins, tandem amino acid repeat has been studied extensively in both animal and plant proteins. Here, a comparative analysis focusing on the proteins having tandem repeats was conducted in 8 microsporidia, including four mammal-infecting microsporidia (Encephalitozoon cuniculi, Encephalitozoon intestinalis, Encephalitozoon hellem and Encephalitozoon bieneusi) and four insect-infecting microsporidia (Nosema apis, Nosema ceranae, Vavraia culicis and Nosema bombycis). We found that the proteins with tandem repeats were abundant in these species...
March 21, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28303622/morphology-and-phylogeny-of-the-soil-ciliate-metopus-yantaiensis-n-sp-ciliophora-metopida-with-identification-of-the-intracellular-bacteria
#2
Atef Omar, Qianqian Zhang, Songbao Zou, Jun Gong
The morphology and infraciliature of a new ciliate, Metopus yantaiensis n. sp., discovered in coastal soil of northern China, were investigated. It is distinguished from its congeners by a combination of the following features: nuclear apparatus situated in the preoral dome; 18-21 somatic ciliary rows, of which three extend onto the preoral dome (dome kineties); three to five distinctly elongated caudal cilia, and 21-29 adoral polykinetids. The 18S rRNA genes of this new species and two congeners, M. contortus and M...
March 17, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28277606/a-novel-spore-wall-protein-from-antonospora-locustae-microsporidia-nosematidae-contributes-to-sporulation
#3
Longxin Chen, Runting Li, Yinwei You, Kun Zhang, Long Zhang
Microsporidia are obligate intracellular parasites, existing in a wide variety of animal hosts. Here, we reported AlocSWP2, a novel protein identified from the spore wall of Antonospora locustae (formerly, Nosema locustae, and synonym, Paranosema locustae), containing four cysteines that are conserved among the homologues of several Microspodian pathogens in insects and mammals. AlocSWP2 was detected in the wall of mature spores via indirect immunofluorescence assay. In addition, immunocytochemistry localization experiments showed that the protein was observed in the wall of sporoblasts, sporonts, and meronts during sporulation within the host body, also in the wall of mature spores...
March 9, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28277604/a-new-heterolobosean-amoeboflagellate-tetramitus-dokdoensis-n-sp-isolated-from-a-freshwater-pond-on-dokdo-island-in-the-east-sea-korea
#4
Jong Soo Park
The genus Tetramitus is a representative amoeboflagellate group within the Heterolobosea, and currently contains over a dozen species. Here, a new heterolobosean amoeboflagellate was isolated from a freshwater pond on Dokdo Island, Korea. The amoebae have eruptive pseudopodia, no uroidal filament, and a nucleus with a central nucleolus. The length and width of the amoebae are 15.5-28.0 μm and 5.4-12.6 μm, respectively. The flagellates are conical, with 4 flagella of equal length (~10 μm). There is a discrete rostrum in the subapical region of the flagellate form...
March 9, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28258655/use-of-highly-specific-molecular-markers-reveals-positive-correlation-between-abundances-of-mesodinium-cf-major-and-its-preferred-prey-teleaulax-amphioxeia-during-red-water-blooms-in-the-columbia-river-estuary
#5
Lydie Herfort, Katie Maxey, Ian Vorhees, Holly M Simon, Kolette Grobler, Tawnya D Peterson, Peter Zuber
In a previous study, Teleaulax amphioxeia-the preferred prey of Mesodinium in the Columbia River estuary-were undetectable within intense annual blooms, suggesting blooms are prey-limited or prey are acquired outside of bloom patches. We used a novel molecular approach specifically targeting the prey (i.e. Unique Sequence Element [USE] within the ribosomal RNA 28S D2 regions of Teleaulax amphioxeia nucleus and nucleomorph) in estuarine water samples acquired autonomously with an Environmental Sample Processor integrated within a monitoring network (ESP-SATURN)...
March 4, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28258618/differences-in-the-detection-of-brdu-edu-incorporation-assays-alter-the-calculation-for-g1-s-and-g2-phases-of-the-cell-cycle-in-trypanosomatids
#6
Marcelo Santos da Silva, Paula Andrea Marin Muñoz, Hugo Aguirre Armelin, Maria Carolina Elias
Trypanosomatids are the etiologic agents of various infectious diseases in humans. They diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary and comparative studies. Here, we show a meticulous study comparing the incorporation and detection of the thymidine analogs BrdU and EdU in Leishmania amazonensis, Trypanosoma brucei, and Trypanosoma cruzi to monitor their DNA replication. We used BrdU- and EdU-incorporated parasites with the respective standard detection approaches: Indirect Immunofluorescence to detect BrdU after standard denaturation (2 M HCl) and "click" chemistry to detect EdU...
March 4, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28245076/in-memoriam-norman-e-williams-1928-2016-pioneer-of-ciliate-architecture
#7
Joseph Frankel, Howard E Buhse
No abstract text is available yet for this article.
February 28, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28231613/genetic-determinism-vs-phenotypic-plasticity-in-protist-morphology
#8
Matthieu Mulot, Katarzyna Marcisz, Lara Grandgirard, Enrique Lara, Anush Kosakyan, Bjorn J M Robroek, Mariusz Lamentowicz, Richard J Payne, Edward A D Mitchell
Untangling the relationships between morphology and phylogeny is key to building a reliable taxonomy, but is especially challenging for protists, where the existence of cryptic or pseudocryptic species makes finding relevant discriminant traits difficult. Here we use Hyalosphenia papilio (a testate amoeba) as a model species to investigate the contribution of phylogeny and phenotypic plasticity in its morphology. We study the response of Hyalosphenia papilio morphology (shape and pores number) to environmental variables in 1) a manipulative experiment with controlled conditions (water level), 2) an observational study of a withinsite natural ecological gradient (water level), and 3) an observational study across 37 European peatlands (climate)...
February 23, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28211202/role-of-modular-polyketide-synthases-in-the-production-of-polyether-ladder-compounds-in-ciguatoxin-producing-gambierdiscus-polynesiensis-and-g-excentricus-dinophyceae
#9
Gurjeet S Kohli, Katrina Campbell, Uwe John, Kirsty F Smith, Santiago Fraga, Lesley L Rhodes, Shauna A Murray
Gambierdiscus, a benthic dinoflagellate, produces ciguatoxins that cause the human illness Ciguatera. Ciguatoxins are polyether ladder compounds that have a polyketide origin, indicating that polyketide synthases (PKS) are involved in their production. We sequenced transcriptomes of Gambierdiscus excentricus and Gambierdiscus polynesiensis and found 264 contigs encoding single domain ketoacyl synthases (KS; G. excentricus: 106, G. polynesiensis: 143) and ketoreductases (KR; G. excentricus: 7, G. polynesiensis: 8) with sequence similarity to type I PKSs, as reported in other dinoflagellates...
February 17, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28211199/description-and-molecular-phylogeny-of-a-novel-hypotrich-ciliate-from-the-soil-of-marche-region-italy-including-notes-on-the-mosyss-project
#10
Daizy Bharti, Santosh Kumar, Antonietta La Terza
The morphology and morphogenesis during cell division of a new stylonychine hypotrich, Rigidocortex quadrinucleatus n. sp., were investigated using live observation and protargol staining. The new species was isolated from soil samples collected from an organic farm in the Marche Region, Italy, in framework of the MOSYSS project. Rigidocortex quadrinucleatus is characterized as follows: cell size about 180 × 80 μm in vivo; four ellipsoidal macronuclear nodules; 44 adoral membranelles: 18 frontal-ventral-transverse cirri consisting of three frontal, four frontoventral, one buccal, three ventral, two pretransverse and five transverse cirri; dorsal kinety 3 with multiple fragmentation; resting cyst with hyaline ridges...
February 17, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28207967/the-synonymy-of-the-toxic-dinoflagellates-prorocentrum-mexicanum-and-p-rhathymum-and-the-description-of-p-steidingerae-sp-nov-prorocentrales-dinophyceae
#11
Fernando Gómez, Dajun Qiu, Senjie Lin
Prorocentrum mexicanum and P. rhathymum are toxicologically important dinoflagellates, but their relationship is not well defined. We investigated strains from Puerto Rico and Brazil by light and scanning electron microscopies. We provide molecular data from a strain isolated near the type locality of P. rhathymum, and the first morphological and molecular data from the South Atlantic Ocean. The rRNA gene (rDNA) sequences of the Puerto Rican and Brazilian strains were identical, and their morphologies fit the description of P...
February 16, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28199765/survey-for-zoonotic-microsporidian-pathogens-in-wild-living-urban-rooks-corvus-frugilegus
#12
Agnieszka Perec-Matysiak, Maria Wesołowska, Kinga Leśniańska, Katarzyna Buńkowska-Gawlik, Joanna Hildebrand, Marta Kicia
Microsporidia are opportunistic pathogens in nature infecting all animal phyla. There is a potential risk of microsporidian spores transmission from urban rooks inhabiting some metropolitan cities to people through casual interactions. The aim of this study was to identify microsporidia species in the droppings of rooks in Wroclaw, Poland. A total of 15 collective sets of droppings were examined using nested-PCR method. Amplification of ITS rRNA gene revealed the presence of Enterocytozoon bieneusi D, Peru 6 and Encephalitozoon hellem 1A genotypes...
February 15, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28187245/molecular-phylogeny-and-ultrastructure-of-aphelidium-desmodesmi-a-new-species-in-aphelida-opisthosporidia
#13
Peter M Letcher, Martha J Powell, Philip A Lee, Salvador Lopez, Micheal Burnett
Aphelids are a diverse group of intracellular parasitoids of algae and diatoms, and are sister to true fungi. Included in four genera, the 14 described species utilize phagocytosis as their mode of nutrition, and the life cycles of these taxa are remarkably similar. However, their putative specificity of host, morphological and ultrastructural features, and genetic divergence have been considered in taxon delineation. Here we examine the host specificity, morphology, ultrastructure, and molecular 18S gene sequence of a new species in Aphelida, Aphelidium desmodesmi sp...
February 10, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28171698/differential-gene-expression-in-five-isolates-of-the-clam-pathogen-quahog-parasite-unknown-qpx
#14
Ewelina Rubin, Arnaud Tanguy, Emmanuelle Pales Espinosa, Bassem Allam
Quahog parasite unknown (QPX) is a thraustochytrid protist that infects the hard clam, Mercenaria mercenaria, causing significant economic losses along the northeastern coasts of North America. Previous investigations noted differences in growth dynamics and virulence in QPX cells from different geographic locations. In order to probe the molecular determinants for these variations, we investigated the transcriptomic profiles of five geographically-distinct QPX isolates using custom 15K 60-mer oligonucleotide arrays...
February 7, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28166378/occurrence-and-genetic-characteristics-of-cryptosporidium-hominis-and-cryptosporidium-andersoni-in-horses-from-southwestern-china
#15
Lei Deng, Wei Li, Zhijun Zhong, Chao Gong, Xuefeng Cao, Yuan Song, Wuyou Wang, Xiangming Huang, Xuehan Liu, Yanchun Hu, Hualin Fu, Min He, Ya Wang, Yue Zhang, Kongju Wu, Guangneng Peng
A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptosporidium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes. Subtyping analysis of the 60-kDa glycoprotein (GP60) gene sequence of C. hominis revealed a new rare subtype Id, named IdA15; only three Id isolates have been reported in humans to date...
February 6, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28166376/morphology-and-morphogenesis-of-a-novel-saline-soil-hypotrichous-ciliate-gonostomum-sinicum-nov-spec-ciliophora-hypotrichia-gonostomatidae-including-a-report-on-the-small-subunit-rdna-sequence
#16
Xiaoteng Lu, Jie Huang, Chen Shao, Saleh A Al-Farraj, Shan Gao
Morphology, cirral pattern, and morphogenesis of the new saline soil hypotrich, Gonostomum sinicum nov. spec. collected from Longfeng Wetland in Daqing, north China, were studied, using detailed live observations and protargol-stained specimens. The new species is characterized as follows: (i) a size in vivo of 100-125 × 30-40 μm, (ii) colorless cortical granules, 0.5 μm across, arranged in short rows, (iii) an adoral zone composed of 28-33 membranelles, (iv) three or four frontoventral rows, one of which extends onto the postoral area, (v) left and right marginal rows composed of 18-27 and 21-35, cirri, respectively, and (vi) usually two transverse cirri...
February 6, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28166371/lineage-specific-and-highly-derived-gene-sequences-among-amoebozoa-revealed-by-the-comparative-analysis-of-transcriptomes-from-twelve-amoebozoan-species
#17
Natalya I Bondarenko, Anton S Bondarenko, Alexey V Smirnov
Amoebozoa represent a difficult group for traditional morphology-based taxonomy. Molecular approaches, such as gene sequencing and DNA barcoding have greatly enhanced our knowledge on the diversity of these organisms. However, metagenomic studies of Amoebozoa still did not provide as impressive results as they did among some other groups of protists. In environmental DNA surveys done on fragments of SSU rDNA gene and other traditional DNA barcodes, Amoebozoa genes normally constitute a minor part of the total gene diversity and represent only the most abundant lineages...
February 6, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28150358/diversity-and-evolution-of-paramoeba-spp-and-their-kinetoplastid-endosymbionts
#18
Shannon J Sibbald, Ugo Cenci, Morgan Colp, Yana Eglit, Charles J O'Kelly, John M Archibald
Members of the genus Paramoeba (including Neoparamoeba) (Amoebozoa) are single-celled eukaryotes of economic and ecological importance because of their association with disease in a variety of marine animals including fish, sea urchins, and lobster. Interestingly, they harbor a eukaryotic endosymbiont of kinetoplastid ancestry, Perkinsela sp. To investigate the complex relationship between Paramoeba spp. and Perkinsela sp., as well as the relationships between different Paramoeba species, molecular data was obtained for four novel isolates...
February 1, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28150355/evaluation-of-systematic-position-of-helicoprorodontids-and-chaeneids-ciliophora-litostomatea-an-attempt-to-break-long-branches-in-18s-rrna-gene-phylogenies
#19
Peter Vďačný, Matej Rataj
Phylogenetic position of some free-living litostomatean taxa has not been correctly determined because of long-branch artifacts in 18S rRNA gene trees. The main aim of this study was to test the effectiveness of various masking algorithms, tree-building techniques, binarization of DNA data as well as combining morphological and molecular data to eliminate long-branch attraction of two problematic groups, helicoprorodontids and chaeneids. Guidance and SlowFaster masking in a combination with PhyloBayesian tree construction erased the artifactual positions of helicoprorodontids and chaeneids...
February 1, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28120360/expanding-the-symbiodinium-dinophyceae-suessiales-toolkit-through-protoplast-technology
#20
Rachel A Levin, David J Suggett, Matthew R Nitschke, Madeleine J H van Oppen, Peter D Steinberg
Dinoflagellates within the genus Symbiodinium are photosymbionts of many tropical reef invertebrates, including corals, making them central to the health of coral reefs. Symbiodinium have therefore gained significant research attention, though studies have been constrained by technical limitations. In particular, the generation of viable cells with their cell walls removed (termed protoplasts) has enabled a wide range of experimental techniques for bacteria, fungi, plants, and algae such as ultrastructure studies, virus infection studies, patch clamping, genetic transformation, and protoplast fusion...
January 25, 2017: Journal of Eukaryotic Microbiology
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