journal
MENU ▼
Read by QxMD icon Read
search

Insect Molecular Biology

journal
https://www.readbyqxmd.com/read/29235691/rna-interference-mediated-functional-characterization-of-aquaporin-genes-in-tribolium-castaneum
#1
X-X Yao, Q-W Meng, G-Q Li
An obvious challenge faced by most terrestrial insects is maintaining water homeostasis in an arid environment. Current research suggests aquaporins may be evolved to meet the challenge. However, up to now, this suggestion has not been verified in any insect that feeds upon solid food with mandibulate mouthparts. In the present paper, nine putative aquaporin genes [Tribolium castaneum big brain, T. castaneum Drosophila integral protein (TcDrip), T. castaneum Pyrocoelia rufa integral protein (TcPrip), T. castaneum aquaporin 12-like, T...
December 13, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29226991/vitellogenin-and-its-receptor-play-essential-roles-in-the-development-and-reproduction-of-the-brown-citrus-aphid-aphis-toxoptera-citricidus
#2
F Shang, J-Z Niu, B-Y Ding, Q Zhang, C Ye, W Zhang, G Smagghe, J-J Wang
Vitellogenin (Vg) and its receptor (VgR) play a key role in the reproductive process and development of insects. Aphids are a group of high-fecundity insect species with pseudoplacental viviparity, but the roles of their Vg and VgR genes have not been investigated yet. The brown citrus aphid, Aphis (Toxoptera) citricidus, is a major insect pest of citrus and the main vector of Citrus tristeza closterovirus. In this study, we identified and characterized these two genes, designated as AcVg and AcVgR, from the brown citrus aphid...
December 11, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29226989/transcriptome-characterization-of-male-accessory-glands-in-ants-to-identify-molecules-involved-in-their-reproductive-success
#3
A Gotoh, S Shigenobu, K Yamaguchi, S Kobayashi, F Ito, K Tsuji
In insects, seminal fluid proteins that are produced by male accessory glands and transferred to females during mating have key functions in sperm competition and sperm physiology that lead to male reproductive success. In ants, male reproductive success also depends on the longevity of sperm stored in the queen's spermatheca because their sexual offspring are usually produced only after a prolonged storage period. We identified genes that were up-regulated in the male accessory glands relative to the bodies of Crematogaster osakensis to characterize the reproductive molecules associated with male reproductive success in ants...
December 11, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29205612/homeodomain-interacting-protein-kinase-phosphorylates-the-drosophila-paired-box-protein-6-pax6-homologues-twin-of-eyeless-and-eyeless
#4
E L Steinmetz, D N Dewald, U Walldorf
Homeodomain-interacting protein kinase (Hipk), the Drosophila homologue of mammalian HIPK2, plays several important roles in regulating differentiation, proliferation, apoptosis, and stress responses and acts as a mediator for signals of diverse pathways, such as Notch or Wingless signalling. The Paired box protein 6 (Pax6) has two Drosophila homologues, Twin of eyeless (Toy) and Eyeless (Ey). Both stand atop the retinal determination gene network (RDGN), which is essential for proper eye development in Drosophila...
December 4, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29193387/the-nuclear-hormone-receptor-e75a-regulates-vitellogenin-gene-al-vg-expression-in-the-mirid-bug-apolygus-lucorum
#5
Y-A Tan, X-D Zhao, Y Sun, D-J Hao, J Zhao, Y-P Jiang, L-X Bai, L-B Xiao
Apolygus lucorum is the predominant pest of Bacillus thuringiensis (Bt) cotton in China. 20-hydroxyecdysone (20E) plays a key role in the reproduction of this insect. To better understand the mechanism underlying 20E-regulated reproduction, the nuclear hormone receptor E75 isoform-A of Ap. lucorum (Al-E75A) was cloned and its expression analysed. A 2241-bp sequence of Al-E75A cDNA encoded an open reading frame of a polypeptide with a predicted molecular mass of 69.04 kDa. Al-E75A mRNA was detected in female adult stages of Ap...
November 29, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29164729/identification-and-characterization-of-a-histamine-binding-lipocalin-like-molecule-from-the-relapsing-fever-tick-ornithodoros-turicata
#6
G Neelakanta, H Sultana, D E Sonenshine, J F Andersen
Lipocalins are low molecular weight membrane transporters that are abundantly expressed in the salivary glands and other tissues of ticks. In this study, we identified a lipocalin-like molecule, designated as otlip, from the soft ticks Ornithodoros turicata, the vector for the relapsing fever causing spirochete Borrelia turicatae. We noted that the expression of otlip was developmentally regulated, with adult ticks expressing significantly higher levels in comparison to the larvae or nymphal ticks. Expression of otlip was evident in both fed and unfed O...
November 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29143394/bactericera-cockerelli-vitellogenin-6-like-a-vitellogenin-without-a-direct-reproductive-function
#7
F Ibanez, X Tang, C Tamborindeguy
Vitellogenin-like proteins are members of the large lipid transfer proteins, a family of proteins involved in reproduction, lipid circulation and immune defences. In this study, we identified a new Bactericera cockerelli vitellogenin-like (Vg-like) transcript, and named it BcVg6-like based on its similarity to Acyrthosiphon pisum Vg6. In silico analyses predicted different conserved domains in BcVg6-like compared with the conventional Ba. cockerelli vitellogenin, BcVg1-like, previously described by our research group...
November 16, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29131435/transgenic-characterization-of-two-silkworm-tissue-specific-promoters-in-the-haemocyte-plasmatocyte-cells
#8
K Zhang, C Li, X Weng, J Su, L Shen, G Pan, D Long, A Zhao, H Cui
Haemocytes play crucial roles in insect metabolism, metamorphosis, and innate immunity. As a model of lepidopteran insects, the silkworm is a useful model to study the functions of both haematopoiesis and haemocytes. Tissue-specific promoters are excellent tools for genetic manipulation and are widely used in fundamental biological research. Herein, two haemocyte-specific genes, Integrin β2 and Integrin β3, were confirmed. Promoter activities of Integrin β2 and Integrin β3 were evaluated by genetic manipulation...
November 13, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29096051/identification-and-initial-characterization-of-novel-neural-immediate-early-genes-possibly-differentially-contributing-to-foraging-related-learning-and-memory-processes-in-the-honeybee
#9
A Ugajin, H Uchiyama, T Miyata, T Sasaki, S Yajima, M Ono
Despite possessing a limited number of neurones compared to vertebrates, honeybees show remarkable learning and memory performance, an example being 'dance communication'. In this phenomenon, foraging honeybees learn the location of a newly discovered food source and transmit the information to nestmates by symbolic abdomen vibrating behaviour, leading to navigation of nestmates to the new food source. As an initial step toward understanding the detailed molecular mechanisms underlying the sophisticated learning and memory performance of the honeybee, we focused on the neural immediate early genes (IEGs), which are specific genes quickly transcribed after neural activity without de novo protein synthesis...
November 2, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29068552/the-anopheles-gambiae-atp-binding-cassette-transporter-family-phylogenetic-analysis-and-tissue-localization-provide-clues-on-function-and-role-in-insecticide-resistance
#10
P Pignatelli, V A Ingham, V Balabanidou, J Vontas, G Lycett, H Ranson
The role of ATP-binding cassette (ABC) transporters in conferring insecticide resistance has received much attention recently. Here we identify ABC transporters differentially expressed in insecticide-resistant populations of the malaria vector, Anopheles gambiae. Although we found little evidence that the orthologues of the multidrug resistance proteins described in other species are associated with resistance in An. gambiae we did identify a subset of ABC proteins consistently differentially expressed in pyrethroid-resistant populations from across Africa...
October 25, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29030999/molecular-characterizations-of-dna-methyltransferase-3-and-its-roles-in-temperature-tolerance-in-the-whitefly-bemisia-tabaci-mediterranean
#11
T-M Dai, Z-C Lü, Y-S Wang, W-X Liu, X-Y Hong, F-H Wan
The Bemisia tabaci Mediterranean (MED) cryptic species is an invasive pest, distributed worldwide, with high ecological adaptability and thermotolerance. DNA methylation (a reversible chromatin modification) is one possible change that may occur within an organism subjected to environmental stress. To assess the effects of temperature stress on DNA methyltransferase 3 (Dnmt3) in MED, we cloned and sequenced BtDnmt3 and identified its functions in response to high and low temperatures. The full-length cDNA of BtDnmt3 was 3913 bp, with an open reading frame of 1962 bp, encoding a 73...
October 14, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29030997/rna-interference-and-functional-characterization-of-a-tergal-gland-alpha-amylase-in-the-german-cockroach-blattella-germanica-l
#12
A J Myers, A D Gondhalekar, M Fardisi, K D Pluchar, K D Saltzmann, G W Bennett, M E Scharf
German cockroach males possess tergal glands that secrete a combination of oligosaccharides, lipids and proteins. Four major proteins occur in the secretion, with one being the 63 kDa alpha-amylase Blattella germanica Tergal Gland protein-1 (BGTG-1). Denaturing and starch gel electrophoresis coupled with peptide sequencing verified amylase activity for the BGTG-1 protein. BGTG-1 gene expression profiles were determined by using quantitative real-time PCR to compare messenger RNA abundance among isolated tissues of males, females and gravid females...
October 14, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29030993/maternal-provision-of-non-sex-specific-transformer-messenger-rna-in-sex-determination-of-the-wasp-asobara-tabida
#13
E Geuverink, E C Verhulst, M van Leussen, L van de Zande, L W Beukeboom
In many insect species maternal provision of sex-specifically spliced messenger RNA (mRNA) of sex determination genes is an essential component of the sex determination mechanism. In haplodiploid Hymenoptera, maternal provision in combination with genomic imprinting has been shown for the parasitoid Nasonia vitripennis, known as maternal effect genomic imprinting sex determination (MEGISD). Here, we characterize the sex determination cascade of Asobara tabida, another hymenopteran parasitoid. We show the presence of the conserved sex determination genes doublesex (dsx), transformer (tra) and transformer-2 (tra2) orthologues in As...
October 14, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28987007/honey-bee-foraging-induces-upregulation-of-early-growth-response-protein-1-hormone-receptor-38-and-candidate-downstream-genes-of-the-ecdysteroid-signalling-pathway
#14
A S Singh, A Shah, A Brockmann
In honey bees, continuous foraging at an artificial feeder induced a sustained upregulation of the immediate early genes early growth response protein 1 (Egr-1) and hormone receptor 38 (Hr38). This gene expression response was accompanied by an upregulation of several Egr-1 candidate downstream genes: ecdysone receptor (EcR), dopamine/ecdysteroid receptor (DopEcR), dopamine decarboxylase and dopamine receptor 2. Hr38, EcR and DopEcR are components of the ecdysteroid signalling pathway, which is highly probably involved in learning and memory processes in honey bees and other insects...
October 7, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28960749/detoxification-of-ivermectin-by-atp-binding-cassette-transporter-c4-and-cytochrome-p450-monooxygenase-6cj1-in-the-human-body-louse-pediculus-humanus-humanus
#15
J H Kim, K J Gellatly, B Lueke, M Kohler, R Nauen, E Murenzi, K S Yoon, J M Clark
We previously observed that ivermectin-induced detoxification genes, including ATP binding cassette transporter C4 (PhABCC4) and cytochrome P450 6CJ1 (CYP6CJ1) were identified from body lice following a brief exposure to a sublethal dose of ivermectin using a non-invasive induction assay. In this current study, the functional properties of PhABCC4 and CYP6CJ1 were investigated after expression in either X. laevis oocytes or using a baculovirus expression system, respectively. Efflux of [(3) H]-9-(2-phosphonomethoxyethyl) adenine ([(3) H]-PMEA), a known ABCC4 substrate in humans, was detected from PhABCC4 cRNA-injected oocytes by liquid scintillation spectrophotometric analysis and PhABCC4 expression in oocytes was confirmed using ABC transporter inhibitors...
September 27, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28940938/downregulation-of-chilo-suppressalis-alkaline-phosphatase-genes-associated-with-resistance-to-three-transgenic-bacillus-thuringiensis-rice-lines
#16
L Qiu, P Wang, T Wu, B Li, X Wang, C Lei, Y Lin, J Zhao, W Ma
Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis are highly toxic to lepidopteran pests. Strains of transgenic rice expressing cry genes have been developed that are resistant to rice pests. Understanding the mode of action of Cry toxins in rice pests will improve our ability to use them effectively as insecticides. In this study, we tested the hypothesis that alkaline phosphatases (ALPs) are involved in Cry1A, Cry2Aa and Cry1Ca toxicity in Chilo suppressalis, an important insect pest of rice crops in China...
September 21, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28776797/micrornas-are-differentially-abundant-during-aedes-albopictus-diapause-maintenance-but-not-diapause-induction
#17
Z A Batz, A C Goff, P A Armbruster
Diapause is a programmed dormancy that allows organisms to tolerate predictable periods of unfavourable conditions by temporarily halting development and reducing metabolism. Diapause is widespread amongst insects and is crucial for allowing organisms to coordinate their growth and reproduction with favourable environmental conditions. Although the adaptive significance of diapause is well understood, the molecular mechanisms underpinning diapause remain unresolved. We performed high-throughput sequencing to investigate the role of microRNAs (miRNAs) in the diapause of the Asian tiger mosquito, Aedes albopictus...
December 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28677913/identification-of-the-prothoracicotropic-hormone-ptth-coding-gene-and-localization-of-its-site-of-expression-in-the-pea-aphid-acyrthosiphon-pisum
#18
M Barberà, D Martínez-Torres
Insect hormones control essential aspects of physiology, behaviour and development in insects. The majority of insect hormones are peptide hormones that perform a highly diverse catalogue of functions. Prothoracicotropic hormone (PTTH) is a brain neuropeptide hormone whose main function is to stimulate the secretion of ecdysone (the moulting hormone) by the prothoracic glands in insect larvae thus playing a key role in the control of moulting and metamorphosis. Moreover, both PTTH release or blockade have been reported to act as a switch to terminate or initiate larval and pupal diapauses...
October 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28654199/imidacloprid-is-hydroxylated-by-laodelphax-striatellus-cyp6ay3v2
#19
R Wang, Y Zhu, L Deng, H Zhang, Q Wang, M Yin, P Song, M E A Elzaki, Z Han, M Wu
Laodelphax striatellus (Fallén) is one of the most destructive pests of rice, and has developed high resistance to imidacloprid. Our previous work indicated a strong association between imidacloprid resistance and the overexpression of a cytochrome P450 gene CYP6AY3v2 in a L. striatellus imidacloprid resistant strain (Imid-R). In this study, a transgenic Drosophila melanogaster line that overexpressed the L. striatellus CYP6AY3v2 gene was established and was found to confer increased levels of imidacloprid resistance...
October 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28640534/dynamics-of-transcriptomic-response-to-infection-by-the-nematode-heterorhabditis-bacteriophora-and-its-bacterial-symbiont-photorhabdus-temperata-in-heliothis-virescens-larvae
#20
R An, K S Suri, J L Jurat-Fuentes, P S Grewal
Entomopathogenic nematodes in the Heterorhabditis genus and their symbiotic Photorhabdus bacteria are important biocontrol agents of insect pests and models for the study of microbe-host interactions. In this work, we used larvae of the tobacco budworm (Heliothis virescens) as a model to study its defensive mechanisms against Heterorhabditis bacteriophora nematodes carrying symbiotic Photorhabdus temperata. We first determined time points of initial nematode entry and release of bacteria into the haemolymph to perform transcriptional analysis of insect gene expression during these steps in the infective process...
October 2017: Insect Molecular Biology
journal
journal
31266
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"