journal
MENU ▼
Read by QxMD icon Read
search

Insect Molecular Biology

journal
https://www.readbyqxmd.com/read/28463435/transcription-factor-aryl-hydrocarbon-receptor-aryl-hydrocarbon-receptor-nuclear-translocator-is-involved-in-regulation-of-the-xenobiotic-tolerance-related-cytochrome-p450-cyp6da2-in-aphis-gossypii-glover
#1
T Peng, X Chen, Y Pan, Z Zheng, X Wei, J Xi, J Zhang, X Gao, Q Shang
The cotton aphid, Aphis gossypii, is one of the most economically important agricultural pests worldwide as it is polyphagous and resistant to many classes of insecticides. Overexpression of the cytochrome P450 monooxygenase (P450) CYP6DA2 has previously been found to be associated with gossypol and spirotetramat tolerance in the cotton aphid. In the present study, the elements located in the promoter region (-357:-343; -250:-241; -113:-104) of CYP6DA2 were shown to control promoter activity, and gossypol induction was observed...
April 30, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28452426/aerobic-respiration-by-haemocyanin-in-the-embryo-of-the-migratory-locust
#2
B Chen, R Ma, D Ding, L Wei, L Kang
It remains unresolved how insect embryos acquire sufficient oxygen to sustain high rates of respiratory metabolism during embryogenesis in the absence of a fully developed tracheal system. Our previous work showed that the two distinct subunits (Hc1 and Hc2) of haemocyanin (Hc), a copper-containing protein, display embryo-specific high expression that is essential for embryonic development and survival in the migratory locust Locusta migratoria. Here we investigated the role of haemocyanins in oxygen sensing and supply in the embryo of this locust...
April 28, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28432783/gene-structure-and-expression-characteristic-of-a-novel-odorant-receptor-gene-cluster-in-the-parasitoid-wasp-microplitis-mediator-hymenoptera-braconidae
#3
S-N Wang, S Shan, Y Zheng, Y Peng, Z-Y Lu, Y-Q Yang, R-J Li, Y-J Zhang, Y-Y Guo
Odorant receptors (ORs) expressed in the antennae of parasitoid wasps are responsible for detection of various lipophilic airborne molecules. In the present study, 107 novel OR genes were identified from Microplitis mediator antennal transcriptome data. Phylogenetic analysis of the set of OR genes from M. mediator and Microplitis demolitor revealed that M. mediator OR (MmedOR) genes can be classified into different subfamilies, and the majority of MmedORs in each subfamily shared high sequence identities and clear orthologous relationships to M...
April 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28432772/studies-on-the-chitin-chitosan-binding-properties-of-six-cuticular-proteins-analogous-to-peritrophin-3-from-bombyx-mori
#4
M Qu, Y Ren, Y Liu, Q Yang
Chitin deacetylation is required to make the cuticle rigid and compact through chitin chain crosslinking. Thus it is presumed that specialized proteins are required to bind deacetylated chitin chains together. However, deacetylated-chitin binding proteins have not ever been reported. In a previous work, six cuticular proteins analogous to peritrophin 3 (CPAP3s) were found to be abundant in the moulting fluid of Bombyx mori. In this study, these BmCPAP3s (BmCPAP3-A1, BmCPAP3-A2, BmCPAP3-B, BmCPAP3-C, BmCPAP3-D1 and BmCPAP3-D2) were cloned and expressed in Escherichia coli and purified using metal-chelating affinity chromatography...
April 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28407384/point-mutations-in-acetylcholinesterase-1-associated-with-chlorpyrifos-resistance-in-the-brown-planthopper-nilaparvata-lugens-st%C3%A3-l
#5
Y Zhang, B Yang, J Li, M Liu, Z Liu
Insecticide resistance frequently results from target-site insensitivity, such as point mutations in acetylcholinesterases (AChEs) for resistance to organophosphates and carbamates. From a field-originated population of Nilaparvata lugens, a major rice pest, a resistant population (R9) was obtained by nine-generation continuous selection with chlorpyrifos. From the same field population, a relatively susceptible population (S9) was also constructed through rearing without any insecticides. Compared to the susceptible strain, Sus [medium lethal dose (LC50 ) = 0...
April 13, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28397990/germline-transformation-of-the-western-corn-rootworm-diabrotica-virgifera-virgifera
#6
F Chu, W Klobasa, P Wu, S Pinzi, N Grubbs, S Gorski, Y Cardoza, M D Lorenzen
The western corn rootworm (WCR), a major pest of maize, is notorious for rapidly adapting biochemically, behaviourally and developmentally to a variety of control methods. Despite much effort, the genetic basis of WCR adaptation remains a mystery. Since transformation-based applications such as transposon tagging and enhancer trapping have facilitated genetic dissection of model species such as Drosophila melanogaster, we developed a germline-transformation system for WCR in an effort to gain a greater understanding of the basic biology of this economically important insect...
April 11, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28390075/a-cytochrome-p450-gene-plays-a-role-in-the-recognition-of-sex-pheromones-in-the-tobacco-cutworm-spodoptera-litura
#7
B Feng, K Zheng, C Li, Q Guo, Y Du
Cytochrome P450 (P450 or CYP) genes are involved in fundamental physiological functions, and might be also associated with the olfactory recognition of sex pheromones in beetles and moths. A P450 gene, Spodoptera litura CYP4L4 (SlituCYP4L4), was cloned for the first time from the antennae of S. litura. SlituCYP4L4 was almost exclusively expressed in the adult stage and predominantly expressed in the adult antennae. In situ hybridization showed that SlituCYP4L4 localized mainly at the base of the long sensilla trichoidea, which responds to sex pheromone components...
April 8, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28374513/identification-and-characterization-of-laodelphax-striatellus-insecta-hemiptera-delphacidae-neutral-sphingomyelinase
#8
Y Zhou, X-W Lin, M-A Begum, C-H Zhang, X-X Shi, W-J Jiao, Y-R Zhang, J-Q Yuan, H-Y Li, Q Yang, C Mao, Z-R Zhu
The neutral sphingomyelinase (nSMase) 1 homologue gene LsSMase was cloned from Laodelphax striatellus, a direct sap-sucker and virus vector of gramineous plants, and expressed via a Bac to Bac baculovirus expression system. The LsSMase-enhanced green fluorescent protein fusion protein was located in the endoplasmic reticulum in a similar manner to mammalian nSMase 1. The biochemical properties of LsSMase were determined in detail. The optimal pH and temperature for recombinant LsSMase were 8 and 37 °C, respectively...
April 4, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28370634/catalase-is-a-determinant-of-the-colonization-and-transovarial-transmission-of-rickettsia-parkeri-in-the-gulf-coast-tick-amblyomma-maculatum
#9
K Budachetri, D Kumar, S Karim
The Gulf Coast tick (Amblyomma maculatum) has evolved as a competent vector of the spotted-fever group rickettsia, Rickettsia parkeri. In this study, the functional role of catalase, an enzyme responsible for the degradation of toxic hydrogen peroxide, in the colonization of the tick vector by R. parkeri and transovarial transmission of this pathogen to the next tick generation, was investigated. Catalase gene (CAT) expression in midgut, salivary glands and ovarian tissues exhibited a 2-11-fold increase in transcription level upon R...
April 1, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28370744/novel-mutations-and-expression-changes-of-acetyl-coenzyme-a-carboxylase-are-associated-with-spirotetramat-resistance-in-aphis-gossypii-glover
#10
Y Pan, E Zhu, X Gao, R Nauen, J Xi, T Peng, X Wei, C Zheng, Q Shang
Acetyl-coenzyme A carboxylase (ACC) catalyses the carboxylation of acetyl-coenzyme A (acetyl-CoA) to produce malonyl-CoA during the de novo synthesis of fatty acids. Spirotetramat, an inhibitor of ACC, is widely used to control a range of sucking insects, including the Aphis gossypii. In the present study, Reverse transcription quantitative real-time PCR (RT-qPCR) results demonstrated that ACC was significantly overexpressed in a laboratory-selected spirotetramat-resistant strain compared with the susceptible strain...
March 31, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28370607/evidence-for-a-role-of-oestrogen-receptor-related-receptor-in-the-regulation-of-male-sexual-behaviour-in-the-moth-agrotis-ipsilon
#11
F Bozzolan, N Durand, E Demondion, T Bourgeois, E Gassias, S Debernard
The oestrogen receptor-related receptors (ERRs) are orphan nuclear receptors that were originally identified on the basis of their close homology to the oestrogen receptors. The three mammalian ERR genes participate in the regulation of vital physiological processes including reproduction, development and metabolic homeostasis. Although unique ERRs have been found in insects, data on the function and regulation of these receptors remain sparse. In the present study, a 2095-bp full-length cDNA encoding an ERR, termed AiERR, was isolated from males of the moth Agrotis ipsilon and deposited in the GenBank database under the accession number KT944662...
March 29, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28247518/three-odorant-binding-proteins-may-regulate-the-behavioural-response-of-chrysopa-pallens-to-plant-volatiles-and-the-aphid-alarm-pheromone-e-%C3%AE-farnesene
#12
REVIEW
Z-Q Li, S Zhang, X-M Cai, J-Y Luo, S-L Dong, J-J Cui, Z-M Chen
Artificial Chrysopa pallens release is a well-known method for suppressing aphids, but it is difficult to establish lacewing populations in the field. Understanding the functions of C. pallens odorant-binding proteins (CpalOBPs) and behavioural responses of C. pallens to plant volatiles and aphid alarm pheromone (E)-ß-farnesene has important implications for population establishment after lacewing release. Based on our previous study, five antennae-enriched CpalOBPs were selected. Sequence alignment and phylogenetic analysis revealed that these five CpalOBPs were Classic OBPs and separated into different clades...
March 1, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28314050/efficacy-of-rna-interference-knockdown-using-aerosolized-short-interfering-rnas-bound-to-nanoparticles-in-three-diverse-aphid-species
#13
M W Thairu, I H Skidmore, R Bansal, E Nováková, T E Hansen, H Li-Byarlay, S A Wickline, A K Hansen
RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA (siRNA)-nanoparticle complexes. By using nanoparticles as a siRNA carrier, the likelihood of cellular uptake is increased, when compared to methods previously used in insects. To determine the efficacy of this RNAi delivery system, siRNAs were aerosolized with and without nanoparticles in three aphid species: Acyrthosiphon pisum, Aphis glycines and Schizaphis graminum...
June 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28299835/multiple-atp-binding-cassette-transporters-are-involved-in-insecticide-resistance-in-the-small-brown-planthopper-laodelphax-striatellus
#14
H Sun, J Pu, F Chen, J Wang, Z Han
ATP-binding cassette (ABC) transporters are membrane-bound proteins involved in the movement of various substrates, including drugs and insecticides, across the lipid membrane. Demonstration of the role of human ABC transporters in multidrug resistance has led to speculation that they might be an important mechanism controlling the fate of insecticides in insects. However, the role of ABC transporters in insects remains largely unknown. The small brown planthopper, Laodelphax striatellus Fallén, has developed resistance to most of the insecticides used for its control...
June 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28105732/comparison-of-the-proliferation-and-excretion-of-bartonella-quintana-between-body-and-head-lice-following-oral-challenge
#15
J H Kim, D J Previte, K S Yoon, E Murenzi, J E Koehler, B R Pittendrigh, S H Lee, J M Clark
Human body and head lice are highly related haematophagous ectoparasites but only the body louse has been shown to transmit Bartonella quintana, the causative agent of trench fever. The mechanisms by which body lice became a vector for B. quintana, however, are poorly understood. Following oral challenge, green fluorescent protein-expressing B. quintana proliferated over 9 days postchallenge with the number of bacteria being significantly higher in whole body vs. head lice. The numbers of B. quintana detected in faeces from infected lice, however, were approximately the same in both lice...
June 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28251713/corrigendum
#16
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/27902874/spodoptera-litura-autophagy-related-protein-1-interacts-with-autophagy-related-protein-5-and-enhances-its-degradation
#17
N Zhang, Y Yang, H Lu, Y Xiang, X Huang, R Hu, Z Chen, W Yuan, R Peng, J Peng, H Ai, K Liu
It is known that the autophagy-related protein 1 (ATG1) plays critical roles in the regulation of autophagy in mammals and yeast, whereas the function of ATG1 in lepidopteran insects is not well elucidated. Here Spodoptera litura ATG1 (SlATG1) and its interactions with other ATG proteins were characterized. Alternative splicing of SlAtg1 produced at least four transcript variants. Over-expression and RNA interference knockdown of SlAtg1 demonstrated that SlATG1 enhanced autophagy. SlATG1A-Green fluorescent protein (GFP) tagged localized in the cytoplasm and formed some punctuate dots, which were colocalized with red fluorescent protein mCherry tagged Spodoptera exigua ATG5 (SeATG5)...
April 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28234413/expanding-the-view-of-clock-and-cycle-gene-evolution-in-diptera
#18
S Chahad-Ehlers, L P Arthur, A L A Lima, J S M Gesto, F R Torres, A A Peixoto, R A de Brito
We expanded the view of Clock (Clk) and cycle (cyc) gene evolution in Diptera by studying the fruit fly Anastrepha fraterculus (Afra), a Brachycera. Despite the high conservation of clock genes amongst insect groups, striking structural and functional differences of some clocks have appeared throughout evolution. Clk and cyc nucleotide sequences and corresponding proteins were characterized, along with their mRNA expression data, to provide an evolutionary overview in the two major groups of Diptera: Lower Diptera and Higher Brachycera...
February 24, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28224717/tissue-specific-expression-and-silencing-phenotypes-of-mitochondrial-phosphate-carrier-paralogues-in-several-insect-species
#19
R Sugahara, A Jouraku, T Nakakura, M Minaba, T Yamamoto, Y Shinohara, H Miyoshi, T Shiotsuki
The mitochondrial phosphate carrier gene (PiC) encodes a membrane protein that mediates the supply of inorganic phosphate from the cytosol into the mitochondrial matrix. This substrate-specific transport system plays an important role in efficient ATP synthesis. Mammals appear to have only one PiC with two alternative splicing variants whose functional differences remain unclear. The present study is the first to characterize the multiple genes that encode PiC in insects. Bombyx mori was found to have two PiC paralogues, one ubiquitous and one testis-specific, the latter seeming to be present only in Lepidoptera...
February 22, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28181326/single-molecule-rna-sequencing-uncovers-trans-splicing-and-improves-annotations-in-anopheles-stephensi
#20
X Jiang, A B Hall, J K Biedler, Z Tu
Single molecule real-time (SMRT) sequencing has recently been used to obtain full-length cDNA sequences that improve genome annotation and reveal RNA isoforms. Here, we used one such method called isoform sequencing from Pacific Biosciences (PacBio) to sequence a cDNA library from the Asian malaria mosquito Anopheles stephensi. More than 600 000 full-length cDNAs, referred to as reads of insert, were identified. Owing to the inherently high error rate of PacBio sequencing, we tested different approaches for error correction...
February 9, 2017: Insect Molecular Biology
journal
journal
31266
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"