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Experimental Dermatology

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https://www.readbyqxmd.com/read/28898476/proceeding-report-of-the-symposium-on-hidradenitis-suppurativa-advances-shsa
#1
Ali Mehdizadeh, Afsaneh Alavi, Raed Alhusayen, Boris Bauer, Falk G Bechara, Marc Bourcier, Alain Brassard, Vahid Djamei, Jan Dutz, Ralph George, Mondana Ghias, Melinda Gooderham, Iltefat Hamzavi, Lauren K Hoffman, Angela Hou, Howard Hu, Alexa Boer Kimball, Mark Kirchhof, Darya Kryzskaya, Maria Del Carmen Liy Wong, Michelle A Lowes, Charles W Lynde, Chelsea McLellen, Errol Prens, Lisette Prens, Tetyana Rogalska, R Gary Sibbald, Mia Sisic, Marcus G Tan, Dennis D Wong
The Symposium on Hidradenitis Suppurativa Advances (SHSA) took place on 22-23 October 2016 at Women's College Hospital in Toronto, Canada. This symposium was a joint meeting of the Canadian Hidradenitis Suppurativa Foundation (CHSF), and the Hidradenitis Suppurativa Foundation (HSF Inc.) founded in USA. The purpose of this meeting was to gather well-known researchers from different disciplines to discuss most recent innovations, practical challenges and potential solutions to issues related to management and care of HS patients...
September 12, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887870/activation-of-nlrp3-signaling-accelerates-skin-wound-healing
#2
Hiroyasu Ito, Ayumu Kanbe, Hiroyasu Sakai, Mitsuru Seishima
The process of skin wound healing involves the following three steps: inflammation, tissue formation, and tissue remodeling. These optimal steps are required for the development of normal wound healing. Recent reports demonstrated that inflammasomes are involved in the innate immune response. In the present study, we examined whether the activation of inflammasomes affects the process of skin wound repair. The skin wound repair model was established using wild-type (WT), NACHT, LRR and PYD domains-containing protein 3 (NALP3) knockout (KO) and ASC-KO mice...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887854/human-tissue-inhibitor-of-metalloproteinases-1-improved-wound-healing-in-diabetes-through-its-anti-apoptotic-effect
#3
Guojuan Lao, Meng Ren, Xiaoyi Wang, Jinglu Zhang, Yanrui Huang, Dan Liu, Hengcong Luo, Chuan Yang, Li Yan
Impaired wound healing accompanies severe cell apoptosis in diabetic patients. Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) was known to have effects on promoting growth and anti-apoptosis for cells. we aimed to determine the actual levels of TIMP-1 and cell apoptosis in: 1) the biopsies of diabetic and non-diabetic foot tissue; 2) the human fibroblasts with or without treatments of Advanced Glycation End Products (AGEs). Next, in both human fibroblasts and the animal model of diabetic rats, to determine the improved levels of cell apoptosis and wound healing after the treatments of either active protein of TIMP-1 or in vivo expression of gene therapy vector-mediated TIMP-1...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887852/cd8-lineage-dendritic-cells-determine-adaptive-immune-responses-to-inflammasome-activation-upon-sterile-skin-injury
#4
Rituparna Chakraborty, Janin Chandra, Shuai Cui, Lynn Tolley, Matthew A Cooper, Mark Kendall, Ian H Frazer
The molecular links between sterile inflammation and induction of adaptive immunity have not been fully identified. Here, we examine how damage associated molecular patterns (DAMPs), as opposed to pathogen associated molecules (PAMPs), regulates the immune response to non-self-antigens presented at the site of the physical injury. Heat applied briefly to the skin invokes sterile inflammation, characterised by local cell death and caspase-1 activation without demonstrably disrupting skin integrity. Co-delivery of ovalbumin (OVA) with heat injury induces OVA-specific CD8(+) T cell responses and this is dependent on caspase-1 activation and MyD88 signalling...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887844/house-dust-mite-allergens-der-f-and-der-p-induce-il-31-production-by-blood-derived-t-cells-from-atopic-dermatitis-patients
#5
Krisztina Szegedi, Amanda van Lier, Pieter C Res, Saskia Chielie, Jan D Bos, Sanja Kezic, Maritza A Middelkamp-Hup, Rosalie M Luiten
No abstract text is available yet for this article.
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887841/evidence-for-a-contributory-role-of-a-xenogeneic-immune-response-in-experimental-epidermolysis-bullosa-acquisita
#6
Markus Niebuhr, Michael Kasperkiewicz, Sebastian Maass, Eva Hauenschild, Katja Bieber, Ralf J Ludwig, Jürgen Westermann, Kathrin Kalies
Autoimmune diseases affect a large fraction of the population in Western countries. To elucidate the underlying causes, autoantibody transfer-induced mouse models have been established that greatly contributed to the understanding of the pathophysiology of these diseases. However, the role of a potentially co-occurring murine xenogeneic immune response to commonly utilized rabbit anti-mouse IgG remains poorly understood. Using the established rabbit anti-mouse type VII collagen (COL7) IgG-induced mouse model of the mucocutaneous blistering disorder epidermolysis bullosa acquisita (EBA), we found in this study a profound T and B cell response along with an altered cytokine expression profile in draining lymph nodes of mice injected with the xenogeneic IgG...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887839/anti-inflammatory-effect-of-astaxanthin-in-phthalic-anhydride-induced-atopic-dermatitis-animal-model
#7
Ju Ho Park, In Jun Yeo, Ji Hye Han, Jeong Won Suh, Hee Pom Lee, Jin Tae Hong
In this study, we investigated anti-dermatitic effects of Astaxanthin (AST) in phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. AD-like lesion was induced by the topical application of 5% PA to the dorsal skin or ear of Hos:HR-1 mouse. After AD induction, 100 μl of 1 mg/ml and 2 mg/ml of AST (10 μg or 20 μg/cm(2) ) was spread on the dorsum of ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-κB (NF-κB) activity...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887824/structural-proteins-of-the-dermal-epidermal-junction-targeted-by-autoantibodies-in-pemphigoid-diseases
#8
REVIEW
Stephanie Goletz, Detlef Zillikens, Enno Schmidt
The dermal-epidermal junction consists of a network of several interacting structural proteins which strengthen adhesion and mediate signaling events. This structural network consists of hemidesmosomal-anchoring filament complexes connecting the basal keratinocytes to the basement membrane. The anchoring filaments in turn interact with the anchoring fibrils to attach the basement membrane to the underlying dermis. Several of these structural proteins are recognized by autoantibodies in pemphigoid diseases, a heterogeneous group of clinically and immunopathologically diverse entities...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887823/variation-of-the-epidermal-expression-of-glucocorticoid-receptor-beta-as-potential-predictive-marker-of-bullous-pemphigoid-outcome
#9
Adrien Brulefert, Sébastien Le Jan, Julie Plée, Anne Durlach, Philippe Bernard, Frank Antonicelli, Aurélie Trussardi-Régnier
Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering disease in Western countries. Although topical and/or systemic glucocorticoids treatment efficacy is widely recognized, up to 30% of patients with BP may undergo a relapse during the first year of treatment. We investigated the protein expression of the total glucocorticoid receptor and GRβ isoform in the skin biopsy specimens from patients with BP, and wondered whether such investigation at baseline provided a tool to predict disease outcome...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887822/paracrine-crosstalk-between-endothelial-cells-and-melanocytes-through-clusterin-to-inhibit-pigmentation
#10
LETTER
Misun Kim, Jiun Lee, Tae Jun Park, Hee Young Kang
No abstract text is available yet for this article.
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28887818/monitoring-uv-induced-signaling-pathways-in-an-ex-vivo-skin-organ-culture-model-using-phospho-antibody-array
#11
C Lenain, B Gamboa, A Perrin, A Séraïdaris, B Bertino, Y Rival, M Bernardi, D Piwnica, B Méhul
We investigated UV-induced signaling in an ex vivo skin organ culture model using phospho-antibody array. Phosphorylation modulations were analyzed in time-course experiments following exposure to solar-simulated UV and validated by western blot analyses. We found that UV induced P-p38 and its substrates, P-ERK1/2 and P-AKT which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho-antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signaling events following perturbations...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28857383/a-human-serum-enriched-medium-formulation-supports-high-viability-and-marker-expression-in-primary-melanocyte-cultures-from-the-outer-root-sheath-and-epidermis
#12
Marie Schneider, Andrea Lohrenz, Michael Cross, Michael C Hacker, Jan C Simon, Vuk Savkovic
Formulating clinically relevant melanocyte cultivation media that maintain the balance between proliferation and maturation to functional melanocytes is a major experimental and regulatory challenge. Within the translation of human melanocytes from the outer root sheath of human hair follicle (HUMORS), we developed a melanocyte medium free of chemical mitogens, chemical melanogenesis enhancers and bovine products, enabling proliferation as well as melanotic differentiation. The formulation involved the replacement of bovine pituitary extract (BPE) and bovine serum (FBS) with human serum (HS) combined with ascorbic acid, CaCl2, epinephrine, L glutamine, insulin and fibroblast growth factor...
August 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28857285/cd11b-deficient-mice-exhibit-an-increased-severity-in-the-late-phase-of-antibody-transfer-induced-experimental-epidermolysis-bullosa-acquisita
#13
LETTER
Fengyuan Deng, Yan Chen, Junfeng Zheng, Qiaoniang Huang, Xuetao Cao, Detlef Zillikens, Frank Petersen, Xinhua Yu
No abstract text is available yet for this article.
August 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833620/eosinophils-as-putative-therapeutic-targets-in-bullous-pemphigoid
#14
REVIEW
Dagmar Simon, Luca Borradori, Hans-Uwe Simon
Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering skin disease and is characterized by the presence of autoantibodies directed against the hemidesmosomal proteins BP180 and BP230 that can be detected in the skin and serum of BP patients. Histologically, the dermal infiltration of eosinophils is obvious. The objective of this review is to present evidence that eosinophils play a key role in the pathogenesis of BP. Eosinophils, together with cytokines and chemokines regulating their production, recruitment and activation, are abundantly present in lesional skin, in blisters and in peripheral blood of patients with BP...
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833576/towards-the-development-of-a-rnai-based-topical-treatment-for-psoriasis-proof-of-concept-in-a-3d-psoriasis-skin-model
#15
Desmet Eline, Mireille Van Gele, Lynda Grine, Katrien Remaut, Jo Lambert
RNA interference has emerged as a powerful tool for therapeutic gene silencing, as it offers the possibility to silence virtually any known pathology-causing gene. However, in vivo delivery of RNAi molecules is hampered by their unfavorable physicochemical characteristics and susceptibility to degradation by endogenous enzymes. To overcome these limitations, we recently developed an elastic liposomal formulation, called DDC642, as topical delivery system of therapeutic RNAi molecules for skin disorders. In this study, we validated the therapeutic efficacy of DDC642-encapsulated RNAi molecules in the treatment of psoriasis using three different in vitro models: a standardized keratinocyte monolayer culture, psoriasis-induced keratinocytes and a psoriasis reconstructed skin model...
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833569/in-vitro-and-in-vivo-models-to-investigate-the-pathomechanisms-and-novel-treatments-for-pemphigoid-diseases
#16
Katja Bieber, Hiroshi Koga, Wataru Nishie
Pemphigoid diseases (PD) are a subgroup of rare acute or chronic autoimmune skin disorders characterized and caused by autoantibodies directed against distinct structural components of the dermal-epidermal junction. Binding of autoantibodies to their targets leads to the formation of blisters and erosions in patients. PDs comprise eight disorders for which the molecular target antigens have been identified. First, we review the available in vitro and ex vivo models for analysis of distinct aspects of the pathogenesis of PDs...
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833566/high-expression-of-b-lymphocyte-stimulator-blys-in-lesional-keratinocytes-of-patients-with-cutaneous-lupus-erythematosus
#17
Joerg Wenzel, Aysche Landmann, Gero Vorwerk, Annegret Kuhn
No abstract text is available yet for this article.
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833556/over-expression-of-p53-explains-isotretinoin-s-teratogenicity
#18
Bodo C Melnik
The precise molecular basis of retinoid embryopathy is yet unknown. This hypothesis predicts that isotretinoin (13-cis retinoic acid), the prodrug of all-trans retinoic acid (ATRA), exaggerates neural crest cell (NCC) apoptosis via upregulation of the pro-apoptotic transcription factor p53, the guardian of the genome. Increased p53 signaling is associated with Treacher Collins-, CHARGE-, and fetal alcohol syndrome, which exhibit dysmorphic craniofacial features resembling retinoid embryopathy. In addition, developmental studies of NCC homeostasis in the zebrafish support the pivotal role of p53...
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833549/regression-of-diffuse-b-cell-lymphoma-of-the-leg-with-intralesional-gentian-violet
#19
Shikha Rao, Robert Morris, Zakiya P Rice, Jack L Arbiser
In this case report, a patient of Primary Cutaneous Diffuse B-cell lymphoma, leg type was treated with intralesional gentian violet as she was judged to be too medically fragile for conventional chemotherapy due to advanced age and multiple serious comorbidities. Gentian violet (Crystal violet/ hexamethyl pararosaniline) is a triphenylmethane dye. It has been shown to have an inhibitory effect on NADPH oxidase, an enzyme family which is found in abundance in reactive oxygen driven tumors such as melanoma and lymphoma...
August 20, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28833504/cytotoxic-anti-melanoma-drugs-suppress-the-activation-of-m2-macrophages
#20
Taku Fujimura, Aya Kakizaki, Yumi Kambayashi, Yota Sato, Kayo Tanita, Chunbing Lyu, Sadanori Furudate, Setsuya Aiba
Together with regulatory T cells (Tregs), tumor-associated macrophages (TAMs) play roles in maintaining the tumor microenvironment. Though cytotoxic anti-melanoma drugs such as dacarbazine (DTIC), nimustine hydrochloride (ACNU), and vincristine (VCR) have been used for the treatment of malignant melanoma as adjuvant therapy in Japan, the detailed mechanisms of their immunomodulatory effects are not fully understood. Since the majority of TAMs are alternatively activated M2 macrophages that favor tumor development, the aim of this study was to elucidate the immunomodulatory effects of these reagents on human monocyte-derived M2 macrophages...
August 20, 2017: Experimental Dermatology
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