Read by QxMD icon Read

Experimental Dermatology

Jae-Hong Kim, Na Young Yoon, Dong Hye Kim, Minyoung Jung, Myungsoo Jun, Hwa-Young Park, Choon Hee Chung, Kyohoon Lee, Sunki Kim, Chang Seo Park, Kwang-Hyeon Liu, Eung Ho Choi
BACKGROUND: The incidence of type 2 diabetes mellitus (DM) has been increasing rapidly, and the disease has become a serious sociomedical problem. Many skin problems, such as xerosis, pruritus, skin infections, and delayed wound healing, that might be related to chronic impairment of skin barrier function decrease the quality of life in DM patients. However, the status of the permeability and antimicrobial barrier of the skin in DM remains unknown. OBJECTIVE: This study aimed to elucidate skin barrier impairment in type 2 DM patients and its patho-mechanisms using classic animal models of type 2 DM...
November 19, 2017: Experimental Dermatology
C D Sadik, J Bischof, N van Beek, A Dieterich, S Benoit, M Sárdy, M Worm, S Meller, R Gläser, D Zillikens, B Homey, J Setterfield, D Minassian, E Schmidt, J Dart, S M Ibrahim
BACKGROUND: Mucous membrane pemphigoid (MMP) is a rare, chronic, and often aggressive subepidermal autoimmune blistering disease potentially affecting several mucous membranes with blisters and secondary erosions and scars. The pathogenesis of MMP is poorly understood, and the contribution of genetic predispositions, other than HLA class II allele variants to MMP, is unknown. OBJECTIVES: The objective of this study is to identify susceptibility genes for MMP in a British cohort of MMP patients...
November 14, 2017: Experimental Dermatology
René G Feichtinger, Roland Lang, Reinhard Geilberger, Florian Rathje, Johannes A Mayr, Wolfgang Sperl, Johann W Bauer, Cornelia Hauser-Kronberger, Barbara Kofler, Michael Emberger
No abstract text is available yet for this article.
November 13, 2017: Experimental Dermatology
LiRong Tan, Shuang Zhao, Wu Zhu, Lisha Wu, Jie Li, MinXue Sheng, Li Lei, Xiang Chen, Cong Peng
Psoriasis is an immune-mediated chronic inflammatory skin disease. Although its pathogenesis is not fully understood, Th17 cells and the cytokines they produce, such as IL-17, IL-22 and IL-23, play critical roles in the pathogenesis of psoriasis. Evidence has demonstrated that psoriasis has some common features, including immune responses (due to Th17 cells) and inflammatory cytokine profiles, with systematic diseases including inflammatory bowel diseases (IBDs) and obesity. Recently, studies have demonstrated that the gut microbiota plays a crucial role in host homeostasis and immune response, particular in Th17 cells, but the role of the gut microbiota in psoriasis remains unclear...
November 11, 2017: Experimental Dermatology
Arti B Patel, Irene Tsilioni, Zuyi Weng, Theoharis C Theoharides
Psoriasis is an autoimmune skin disease characterized by keratinocyte hyper-proliferation and chronic inflammation. The pathogenesis of psoriasis involves pro-inflammatory cytokines, such as tumor necrosis factor (TNF), but the mechanism of keratinocyte activation is not well understood. Here we show that TNF (10 or 50 ng/mL) stimulates significant (p<0.0001) gene expression and secretion of pro-inflammatory IL-6, CXCL8 and VEGF from both cultured human HaCaT and normal epidermal human keratinocytes (NHEKs)...
November 4, 2017: Experimental Dermatology
Franz J Wortmann, Gabriele Wortmann
A deconvolution process has been developed for curves obtained by Differential Scanning Calorimetry in water for Merino wool and the main ethnic hair types. This enables estimation of the fractions of ortho- and para-type cell groups. The results also indicate that hair may contain a further, low-sulphur sub-group of ortho-type cells. The sizes of the major cell fractions are in line with expectations from microscopical investigations. The fractions are comparable for hair types and no consistent association between cell type fractions and hair curvature is observed...
November 4, 2017: Experimental Dermatology
Hans I-Chen Harn, Rei Ogawa, Chao-Kai Hsu, Michael W Hughes, Ming-Jer Tang, Cheng-Ming Chuong
Following skin wounding, the healing outcome can be; regeneration, repair with normal scar tissue, repair with hypertrophic scar tissue, or the formation of keloids. The role of chemical factors in wound healing has been extensively explored, and while there is evidence suggesting the role of mechanical forces, its influence is much less well defined. Here we provide a brief review on the recent progress of the role of mechanical force in skin wound healing by comparing laboratory mice, African spiny mice, fetal wound healing, and adult scar keloid formation...
November 4, 2017: Experimental Dermatology
W V J Hariton, A Galichet, T Vanden Berghe, A M Overmiller, M G Mahoney, W Declercq, E J Müller
No abstract text is available yet for this article.
November 3, 2017: Experimental Dermatology
Delphine Giusti, Sébastien Le Jan, Gregory Gatouillat, Philippe Bernard, Bach Nga Pham, Frank Antonicelli
Bullous pemphigoid (BP) is the most common autoimmune sub-epidermal blistering disease of the skin. Investigation of the BP-associated pathophysiological processes during the last decades showed that the generation of auto-antibodies directed against the hemidesmosome proteins BP180 and BP230, a hallmark of the BP-associated autoimmune response, leads to the recruitment of inflammatory immune cells at the dermal-epidermal junction, and subsequently to the release of a large amount of inflammatory molecules involved in blister formation...
November 3, 2017: Experimental Dermatology
Hanqian Zhang, Maja Ericsson, Marie Virtanen, Simone Weström, Carolina Wählby, Anders Vahlquist, Hans Törmä
Immunofluorescence (IF) and in situ proximity ligation assay (isPLA) are techniques that are used for in situ protein expression and colocalization analysis, respectively. However, an efficient quantitative method to analyze both IF and isPLA staining on skin sections is lacking. Therefore, we developed a new method for semi-automatic quantitative layer-by-layer measurement of protein expression and colocalization in skin sections using the free open-source software CellProfiler. As a proof of principle, IF and isPLA of ichthyosis-related proteins TGm-1 and SDR9C7 were examined...
November 1, 2017: Experimental Dermatology
Yuriko Yamamura, Shin Morizane, Takenobu Yamamoto, Jun Wada, Keiji Iwatsuki
Double-stranded RNA (dsRNA) sensors including TLR3, MDA5, and RIG-I are expressed in epidermal keratinocytes, and play an important immunological role by enhancing various innate and adaptive immune responses. Although the role of elevated extracellular calcium concentration in keratinocyte differentiation is well understood, the effect of high calcium on dsRNA sensors is not well studied. We investigated alterations in dsRNA sensor expression and antiviral activity induced by a high extracellular concentration of calcium in epidermal keratinocytes...
October 31, 2017: Experimental Dermatology
Xiu-Qiong Fu, Ji-Yao Chou, Ting Li, Pei-Li Zhu, Jun-Kui Li, Cheng-Le Yin, Tao Su, Hui Guo, Kin-Wah Lee, Muhammad Jahangir Hossen, Gui-Xin Chou, Zhi-Ling Yu
No abstract text is available yet for this article.
October 27, 2017: Experimental Dermatology
Kirsten Dietze-Schwonberg, Beate Lorenz, Susanna Lopez Kostka, Beatrix Schumak, André Gessner, Esther von Stebut
No abstract text is available yet for this article.
October 27, 2017: Experimental Dermatology
G C van de Glind, A N Bastidas Torres, W H Zoutman, S A J van der Zeeuw, S M Kielbasa, F R de Gruijl, C P Tensen
We studied Lgr6(+) stem cells in experimental UV carcinogenesis in hairless mice. For further characterisation through RNA-seq, these stem cells were isolated by FACS from transgenic hairless mice bearing an EGFP-Ires-CreERT2 reporter cassette inserted into exon-1 of the Lgr6 gene (purity confirmed by human ERT2 expression). Between Lgr6/EGFP(+) and Lgr6/EGFP(-) basal cells (Tg/wt) 682 RNAs were differentially expressed, indicating stemness and expression of cancer-related pathways in Lgr6/EGFP(+) cells. We discovered that suspected 'Lgr6 null' mice (Tg/Tg) expressed RNA of an Lgr6 isoform (delta-Lgr6, lacking 74 N-terminal aa) which could be functional and explain the lack of a phenotype...
October 7, 2017: Experimental Dermatology
Natalia Kunz, Eva Hauenschild, Sebastian Maass, Kai-Uwe Kalies, Matthias Klinger, Melanie Barra, Lars Hecht, Franz Helbig, Stefan Soellner, Charles C Caldwell, Ralf J Ludwig, Jürgen Westermann, Kathrin Kalies
Previous reports have demonstrated that cell-derived nanoparticles (CDNPs) composed of bovine or porcine protein complexes exerted therapeutic effects against viral infections and cancer in mice and humans. Based on these observations, we asked whether CDNPs would improve inflammatory skin disorders. To address this, we utilized two distinct mouse models of cutaneous inflammation: the autoimmune skin blistering disease epidermolysis bullosa acquisita (EBA) as an example of an autoantibody-induced cutaneous inflammation, and Leishmania major (L...
September 23, 2017: Experimental Dermatology
Eun Wook Choi, Min Koo Seo, Eun Young Woo, Suk Hyung Kim, Eun Joo Park, Sue Kim
No abstract text is available yet for this article.
September 23, 2017: Experimental Dermatology
Sarolta Kárpáti, Miklós Sárdy, Krisztián Németh, Balázs Mayer, Neil Smyth, Mats Paulsson, Heiko Traupe
Transglutaminases (TGs) are structurally and functionally related enzymes that modify the posttranslational structure and activity of proteins or peptides, and thus are able to turn on or switch off their function. Depending on location and activities, TGs are able to modify the signaling, the function and the fate of cells and extracellular connective tissues. Beside mouse models also human diseases enable us to appreciate the function of various TGs. In this paper skin diseases induced by genetic damages or autoimmune targeting of these enzymes will be discussed...
September 23, 2017: Experimental Dermatology
Yurie Shimoda-Komatsu, Yohei Sato, Yoshimi Yamazaki, Ryo Takahashi, Tetsuo Shiohara
BACKGROUND: Although atopic dry skin is believed to be caused by defects in skin genes important for maintaining skin barrier function, the role of sweat in atopic dermatitis (AD) has been apparently underestimated. Given the great capacity of sweat to maintain and increase skin hydration, defective sweating responses may be a logical place to look for changes that predispose individuals to the disease. METHODS: We investigated how disease process and sweating defects progress from early asymptomatic stages to the onset of clinically apparent disease by employing the impression mold technique, which allows an accurate quantification of individual sweat gland/duct activity in relation to skin surface topography...
September 23, 2017: Experimental Dermatology
Ali Mehdizadeh, Afsaneh Alavi, Raed Alhusayen, Boris Bauer, Falk G Bechara, Marc Bourcier, Alain Brassard, Vahid Djamei, Jan Dutz, Ralph George, Mondana Ghias, Melinda Gooderham, Iltefat Hamzavi, Lauren K Hoffman, Angela Hou, Howard Hu, Alexa Boer Kimball, Mark Kirchhof, Darya Kryzskaya, Maria Del Carmen Liy Wong, Michelle A Lowes, Charles W Lynde, Chelsea McLellen, Errol Prens, Lisette Prens, Tetyana Rogalska, R Gary Sibbald, Mia Sisic, Marcus G Tan, Dennis D Wong
The Symposium on Hidradenitis Suppurativa Advances (SHSA) took place on 22-23 October 2016 at Women's College Hospital in Toronto, Canada. This symposium was a joint meeting of the Canadian Hidradenitis Suppurativa Foundation (CHSF), and the Hidradenitis Suppurativa Foundation (HSF Inc.) founded in USA. The purpose of this meeting was to gather well-known researchers from different disciplines to discuss most recent innovations, practical challenges and potential solutions to issues related to management and care of HS patients...
September 12, 2017: Experimental Dermatology
Hiroyasu Ito, Ayumu Kanbe, Hiroyasu Sakai, Mitsuru Seishima
The process of skin wound healing involves the following three steps: inflammation, tissue formation, and tissue remodeling. These optimal steps are required for the development of normal wound healing. Recent reports demonstrated that inflammasomes are involved in the innate immune response. In the present study, we examined whether the activation of inflammasomes affects the process of skin wound repair. The skin wound repair model was established using wild-type (WT), NACHT, LRR and PYD domains-containing protein 3 (NALP3) knockout (KO) and ASC-KO mice...
September 8, 2017: Experimental Dermatology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"