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Experimental Dermatology

Marta Stawczyk-Macieja, Katarzyna Wertheim-Tysarowska, Rafał Jakubowski, Aneta Szczerkowska-Dobosz, Magdalena Krygier, Aleksandra Wilkowska, Justyna Sawicka, Wiesław Nowak, Jerzy Bal, Roman Nowicki
Epidermolysis bullosa is a group of inherited blistering skin diseases resulting in most cases from missense mutations in KRT5 and KRT14 genes encoding the basal epidermal keratins 5 and 14. Here, we present a patient diagnosed with a localized subtype of epidermolysis bullosa simplex caused by a heterozygous mutation p.Ala428Asp in the KRT5 gene, that has not been previously identified. Moreover, a bioinformatic analysis of the novel mutation was performed, showing changes in the interaction network between the proteins...
September 21, 2018: Experimental Dermatology
Naglaa Fathi Agamia, Osama Mohamed Hussein, Rania ElSaied Abdelmaksoud, Dina Mohamed Abdalla, Iman Mamdouh Talaat, Eiman Ibrahim Zaki, Amira El Tawdy, Bodo C Melnik
Oral isotretinoin is the most effective anti-acne drug with the strongest sebum-suppressive effect caused by sebocyte apoptosis. It has been hypothesized that upregulation of nuclear FoxO transcription factors and p53 mediate isotretinoin-induced sebocyte apoptosis in vivo. It is the aim of our study to analyse the distribution of the pro-apoptotic transcription factors FoxO1 and FoxO3 in the nuclear and cytoplasmic compartments of human sebocytes in vivo before and during isotretinoin treatment of acne patients...
September 21, 2018: Experimental Dermatology
Yi-Ju Chen, Hsiu J Ho, Ching-Hung Tseng, Zi-Lun Lai, Jeng-Jer Shieh, Chun-Ying Wu
OBJECTIVES: The intestinal microbiota has been known to involve in obesity and host immune response. We aimed to investigate the intestinal microbiota and potential genetic function in relation to clinical presentation in psoriasis patients. METHODS: Fecal microbiota and predicted genetic function inferred from high-throughput 16S ribosomal RNA sequencing were analyzed between psoriasis (n=32) and age-, gender- and body mass index (BMI)-matched non-psoriasis subjects (n=64), from a referral medical center...
September 20, 2018: Experimental Dermatology
Lingjie Tang, Jiaman Wang, Jingna Zhu, Yanhua Liang
SHARPIN is an important component of the linear ubiquitin chain assembly complex (LUBAC). Loss of function of SHARPIN results in eosinophilic inflammation in multiple organs including skin with Th2 -dominant cytokines and dysregulated development of lymphoid tissues in mice. The clinicpathological features are similar to atopic dermatitis (AD) in humans. In order to investigate the potential role of SHARPIN in the pathogenesis of AD, we performed genetic association study of the genotypes and haplotypes as well as SHARPIN's expression between AD cases and controls...
September 19, 2018: Experimental Dermatology
Jin Hee Kim, Hyun Cheol Bae, Jaehyung Kim, Hana Lee, Woo-In Ryu, Eui Dong Son, Tae Ryong Lee, Sang Hoon Jeong, Sang Wook Son
Hypoxia-inducible factor-1α (HIF-1α) has been reported to be up-regulated in psoriatic epidermis, resulting in increased proliferation and abnormal differentiation of human keratinocytes (KCs). However, the role of HIF-1a in psoriatic epidermis, which is mainly composed of KCs, is poorly understood. Here, we show that morphogenic protein 6 (BMP6) is downregulated when HIF-1α is upregulated in patients with psoriasis skin lesions. HIF-1α overexpression in primary human KCs promoted proliferation and inhibited terminal differentiation...
September 19, 2018: Experimental Dermatology
Peng-Yang Hsu, Hui-Ju Yang, Tao-Hsiang Yang, Che-Chun Su
Psoriasis is a chronic autoimmune disease with keratinocyte activation and lymphocyte infiltration in the skin. Our previous study found that 5-hydroxytrytophan (5(OH)Trp), a tryptophan metabolite alleviated collagen-induced arthritis and suppressed cytokine production. In this study, we evaluated the effects of 5(OH)Trp in a mouse model for psoriasiform dermatitis, induced by imiquimod (IMQ). We showed that 5(OH)Trp significantly reduced the cumulative scores, epidermal thickness, and ki-67 expression in the skin...
September 16, 2018: Experimental Dermatology
N De Carvalho, J Welzel, S Schuh, L Themstrup, M Ulrich, G B E Jemec, J Holmes, S Kaleci, J Chester, L Bigi, S Ciardo, G Pellacani
BACKGROUND: Malignant melanoma is an aggressive skin cancer, which can lead to metastasis development. Vascularization enhancement is fundamental for tumor growth, worsening the prognosis. Dynamic Optical Coherence Tomography (D-OCT) enables the in vivo evaluation of vascular patterns in skin lesions. OBJECTIVE: In vivo evaluation of the melanoma vessel morphology by means of D-OCT and correlation with Breslow index. METHODS: Retrospective analysis of histologically proven melanomas, evaluated by D-OCT at three different depths (150, 300 and 500 μm) was performed...
September 15, 2018: Experimental Dermatology
Kyle T Amber, Alex Chernyavsky, Arianna F Agnoletti, Emanuele Cozzani, Sergei A Grando
Cutaneous deposition of eosinophil degranulation proteins is a major feature of eosinophil rich cutaneous diseases including bullous pemphigoid (BP). We sought to better understand the effect of two of these proteins -eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN), on human keratinocytes using the Het-1A cell line. To evaluate expression of key cytokines and chemokines observed in BP as well as metal metalloprotease 9 (MMP9), we performed qPCR and in-cell Western assays on cells treated with either ECP or EDN...
September 15, 2018: Experimental Dermatology
Ifa Etesami, Hassan Seirafi, Narges Ghandi, Hamzeh Salmani, Maedeh Arabpour, Ali Nasrollahzadeh, Amir Teimourpour, Maryam Daneshpazhooh, Mohammad Keramatipour
Recently, ST18 polymorphism has played a role in increasing the risk of pemphigus among some populations such as Egyptian and Jewish. In addition, a variant within the ST18 promoter gene was shown to induce ST18 up-regulation and cytokine secretion leading to keratinocyte susceptibility to anti-desmoglein antibodies. Thus, the present study aimed to assess the ST18 single nucleotide polymorphisms (SNP) relationship with pemphigus, disease severity, and family history among Iranian population. A total of 111 pemphigus patients and 201 healthy controls were genotyped for three ST18 SNPs rs2304365, rs10504140, rs4074067 by using TETRA-ARMS PCR method...
September 7, 2018: Experimental Dermatology
Kayo Tanita, Taku Fujimura, Yota Sato, Chunbing Lyu, Setsuya Aiba
Minocycline/tetracycline is clinically used for the treatment of bullous pemphigoid (BP), and its clinical benefits are superior to those of prednisolone when considering adverse events. Although the clinical benefits of minocycline/tetracycline are well known, its immunosuppressive mechanisms are still unclear. In this study, we investigated the immunomodulatory effects of traditional anti-BP drugs (minocycline, nicotinic acid amide, dexamethasone, and cyclosporine) on CD163+ M2 macrophages in vitro, with special focus on the production of CCL18 and CCL22, both of which are produced by CD163+ M2 macrophages in the lesional skin of BP and are increased in the serum of BP patients...
September 7, 2018: Experimental Dermatology
Jordan Yap, William Yolland, Philipp Tschandl
While convolutional neural networks (CNNs) have successfully been applied for skin lesion classification, previous studies have generally considered only a single clinical/macroscopic image and output a binary decision. In this work we have presented a method which combines multiple imaging modalities together with patient metadata to improve the performance of automated skin lesion diagnosis. We evaluated our method on a binary classification task for comparison with previous studies as well as a 5 class classification task representative of a real world clinical scenario...
September 5, 2018: Experimental Dermatology
Simon Müller, Marilena Fischer, Simon Herger, Corina Nüesch, Christian Egloff, Peter Itin, Lucian Cajacob, Oliver Brandt, Annegret Mündermann
Mechanically induced itch is an important cofactor in many patients with chronic itch. However, studying mechanical itch in a controlled environment is challenging because it is difficult to evoke. We investigated the use of whole body vibration exercise (WBV) exercise, a training method used for musculoskeletal rehabilitation, to experimentally evoke mechanical itch. Mild to severe itch ascending from the soles to the groins was evoked in 16 of 20 healthy participants. We observed a characteristic on/off itch crescendo pattern reflecting the alternating intervals of vibration and no vibration...
August 27, 2018: Experimental Dermatology
Ge Shi, Pei-Yu Liao, Xiao-Lin Cai, Xiao-Xue Pi, Man-Feng Zhang, Shi-Jie Li, Juan-Hua Quan, Yi-Ming Fan
Forkhead box-O1 (FoxO1) is a key nutrient- and growth factor-dependent regulator of metabolism, but its functional role in human primary keratinocytes (HPKs) is less known. To investigate the role of FoxO1 in HPKs and effect of insulin-like growth factor 1 (IGF-1) and isotretinoin on FoxO1 expression. HPKs were treated with 1.2 mM calcium chloride, 1-20 ng/ml IGF-1 and 0.1-10 μM isotretinoin. Recombinant adenovirus expressing FoxO1 or FKHR shRNA lentivirus transfection was introduced to upregulate or silence FoxO1 expression...
August 25, 2018: Experimental Dermatology
Nadine A Pringle, Maryna van de Venter, Trevor C Koekemoer
Protein glycation has been implicated in skin aging and several other disease states, however the slow rate of glycation end product formation makes in vitro studies challenging and often impractical. Gelatin, a denatured form of collagen, was identified as a convenient glycation surrogate amenable to cell culture conditions. The suitability of glycated gelatin to model the effects of AGE formation was verified using RAW 264.7 macrophages which revealed a remarkable correlation to previously documented effects...
August 23, 2018: Experimental Dermatology
Supriya Bhardwaj, Alka Bhatia, Muthu Sendhil Kumaran, Davinder Parsad
Cytokines regulate immune response and inflammation and play an important role in depigmentation process of an autoimmune disease, vitiligo. We sought to determine how inflammatory cytokines influence the progression of vitiligo, and based on that, we develop a logical therapeutic intervention using primary melanocyte culture. Melanocytes were cultured and exposed to IL-17A, IL-1β, IFN-γ and TGF-β for 4 days. Melanocytes proliferation, tyrosinase assay and melanin content were measured. Real-Time PCR was used to analyse mRNA expression of genes specific for melanocytes growth and pigmentation...
August 18, 2018: Experimental Dermatology
Hui Yang, Xia Li, Feng Xue, Qunli Xia, Xin Zhao, Dixin Wang, Lihong Chen, Hua Cao, Han Xu, Xiaoyan Shen, Weiru Yuan, Xiaoqing Zhao, Ruofei Shi, Jie Zheng
Human prolactin (PRL) is a well-known hormone for pituitary of lactation and reproduction, but it also has immunostimulatory effect in some inflammatory or autoimmune diseases including psoriasis, which has not been well elucidated. This study aimed to determine the relationship between PRL and psoriasis through clinical case-control studies, and explore the function of PRL in the pathogenesis of imiquimod (IMQ)-induced psoriasis-like mouse model. Serum from patients with psoriasis vulgaris (PsV), patients with erythrodermic psoriasis, and healthy controls (HCs) were collected for PRL test...
August 18, 2018: Experimental Dermatology
Jessica Szu-Chia Chen, Anes Yang, Dédée F Murrell
BACKGROUND: Osteopenia and osteoporosis are one of the many comorbidities in patients with Epidermolysis Bullosa (EB). Current literature on the prevalence of osteoporosis in EB is scarce. OBJECTIVE: This review will analyse the current literature in the field of patients with compromised bone health in EB and any articles on the prevalence of such diseases in EB groups. METHODS: A systematic search for articles related to bone health and epidermolysis bullosa (EB) (1946-2017) was performed on seven databases: MEDLINE, Embase and EBM, PubMed, ProQuest, Scopus and Web of Science...
August 17, 2018: Experimental Dermatology
Kerstin Wolk, Andrea Braun, Sandra Franz, Evelyn Gaffal, Dennis Gerloff, Robert Sabat
No abstract text is available yet for this article.
August 14, 2018: Experimental Dermatology
Stefanie Gilles, Cezmi Akdis, Roger Lauener, Peter Schmid-Grendelmeier, Thomas Bieber, Georg Schäppi, Claudia Traidl-Hoffmann
Allergies are usually referred to as type I hypersensitivity reactions against innocuous environmental antigens, characterized by a Th2/IgE-dominated inflammation. They can manifest themselves in various organs, such as skin, gastro-intestinal and respiratory tract, and comprise diseases as diverse as allergic rhinitis and conjunctivitis, bronchial asthma, oral allergy syndrome, food allergy, urticaria and atopic eczema, but also anaphylactic shock. Within the last decades there was a significant global increase in allergy prevalence, which has been mostly attributed to changes in environment and life style...
August 12, 2018: Experimental Dermatology
Kaiyuan Ji, Junzhen Zhang, Ruiwen Fan, Shanshan Yang, Changsheng Dong
Melanoma is a highly invasive and metastatic malignant skin tumor with poor prognosis. Although several widely studied pure melanoma cell lines are available, the precise mechanism underlying transformation of melanocyte to melanoma remains unclear. Long non-coding RNAs (lncRNAs) represent a vast category of non-coding RNA molecules, and increasing evidence suggests that lncRNAs are crucial for various biological processes, including those in the skin. Herein, lncRNA sequencing was performed on an Illumina HiSeq platform to identify lncRNAs expressed differently in murine B16 melanoma cells compared to normal mouse melanocytes...
August 12, 2018: Experimental Dermatology
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