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Cellular and Molecular Biology

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https://www.readbyqxmd.com/read/30213299/association-between-bim-polymorphism-and-lung-cancer-outcomes-a-meta-analysis
#1
Xiao-Feng Li, Li-Xin Wu, Hua-Fei Chen, You-Cai Zhu, Wen-Xian Wang, Chun-Wei Xu, Xue-Ping Lin, Dong-Fang Xie, Kai-Qi Du
Accumulating evidences have indicated that BIM expression largely decides the development of lung cancer and outcome of EGFR-mutant lung cancers after TKI treatments. BIM polymorphism is a 2,903-bp deletion in the second exon. To clarify the relationship between this BIM polymorphism and clinical outcomes of lung cancers, we conducted this meta-analysis and observed the survival and responses to TKIs. Sixteen cohort studies, covering 4393 WT and 916 BIM deletion patients were included. Overall, BIM deletion polymorphism was associated with significantly shorter progression-free survival (PFS) and slightly shorter overall survival (OS), compared to the WT group...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213298/the-influence-of-dimethyl-sulfoxide-dmso-on-metabolic-activity-and-morphology-of-melanoma-cell-line-wm-266-4
#2
Polonca Ferk, Barbara Dariš
Dimethyl sulphoxide (DMSO) is widely used as a solvent in biomedical research, regularly in concentrations up to 1%. Nevertheless, little is known about the effect of different DMSO concentration on WM-266-4 metastatic melanoma cells, which are often used in melanoma research. Due to resistance of melanoma cells high concentrations of cytotoxic substances soluble in DMSO are used in vitro tests. Consequently, total DMSO concentration often exceeds 1%. The aim of our study was to test the metabolic activity and morphology of WM-266-4 cells exposed to selected DMSO concentrations for different period of time...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213297/piperlongumine-as-anticancer-agent-the-story-so-far-about-killing-many-birds-with-one-stone
#3
Ammad Ahmad Farooqi, Rukset Attar, Ilhan Yaylim, Muhammad Zahid Qureshi, Milica Todorovska, Gökçe Şeker Karatoprak, Saeideh Najafi, Uteuliyev Yerzhan Sabitaliyevich, Tokmurziyeva Gulnara Zhenisovna, Damião Pergentino De Sousa, Xiukun Lin
Piperlongumine is a biologically and pharmacologically active constituent of the plant Piper longum. This compound is gradually gaining attention because of its ability to inhibit/prevent different cancers. Modern era of molecular oncology is incomplete without ground-breaking discoveries made in the field of cell signaling pathways. High-throughput technologies have considerably improved our understanding about wide ranging signal transduction cascades which play crucial role in cancer development and progression...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213296/ctla-4-49a-g-and-nod2-card15-n852s-polymorphisms-with-inflammatory-bowel-disease-in-turkish-patients
#4
Songül Budak Diler, Serkan Yaraş
Crohn's disease (CD) and ulcerative colitis (UC) are the major types of inflammatory bowel disease (IBD) and exhibit similar clinical features and epidemiology. The main objective of this study was to analyze the correlation between the CTLA-4 gene +49A/G polymorphism and the NOD2/CARD15 gene N852S polymorphism in Turkish patients with IBD using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. In this study, we evaluated the frequency of the CTLA-4 (+49A/G) and NOD2/CARD15 (N852S) polymorphisms in 62 patients with CD, 76 patients with UC, and 152 healthy individuals...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213295/contrast-enhanced-ultrasound-in-diagnosis-of-endometrial-carcinoma-and-endometrial-hyperplasia
#5
Qichen Su, Zhen Sun, Guorong Lv
The present study was aimed to compare application of contrast enhanced ultrasound (CEUS) in diagnosis of endometrial carcinoma (EC) and endometrial hyperplasia (EH). A total of 81 patients with EC and simple EH were selected in this study. Among all patients, 39 cases were diagnosed as EC and 42 cases were diagnosed with EH. All patients were diagnosed by CEUS examination. The diagnosis of EC and EH was also confirmed by endometrial biopsy. CEUS was conducted for all patients. Eendometrial thickness was measured and the mean arrival time, time-to-peak, enhancement time, arrival intensity, peak intensity, enhancement intensity, rising rate, washout half-time and clearance half-time were recorded...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213294/-174-g-c-polymorphism-of-interleukin-6-gene-is-not-significantly-different-in-turkish-professional-short-and-long-distance-runners
#6
Secil Arica, Huseyin Avni Solgun, Ayse Seda Demirel Dengi, Enver Bakmaz, Teoman Akçay, Sezgin Kapici, Canan Sercan, Korkut Ulucan
Interleukin-6 (IL-6) is a kind of multifunctional cytokine and involved in mediating muscle repair metabolism, and therefore athletic capacity.  Muscular and circulating IL-6 levels increase in response to physical exercise. Responsible gene coding for IL-6 has a functional polymorphism in its promoter region, -174 G/C (rs1800795). We aimed to analyze the association of G allele and GG genotype in Turkish professional athletes and compare the allelic and genotypic difference between short distance and long distance runners...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213293/effect-of-gdf11-on-proliferation-and-apoptosis-of-esophageal-cancer-cells
#7
Biao Gu, Qi Wang, Weijie Dai, Gang Ma, Yaqi Song, Qinquan Wu
This study was aimed at investigating the effect of growth differentiation factor 11 (GDF11) on the proliferation and apoptosis of esophageal cancer cells. Serum levels of GDF11 in esophageal cancer patients were determined with ELISA kits, and the correlation between serum GDF11 and pathological features of esophageal cancer were determined. The effect of recombinant GDF11 on the growth of esophageal cancer cells was measured by CCK6 method. In order to investigate the effect of recombinant GDF11 on the proliferation and apoptosis of esophageal cancer cells, the expression of apoptosis-promoting protein Bax and proliferative-associated protein Bcl-2 in esophageal cancer cells were determined using western blot...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213292/the-effects-of-apelin-on-myometrium-contractions-in-pregnant-rats
#8
Emine Kacar, Zubeyde Ercan, Ihsan Serhatlioglu, Aysegul Sumer, Haluk Kelestimur, Selim Kutlu
Apelin, which is a new hormone, is secreted especially in the brain by hypothalamus as well as by many other organs like the stomach, fat tissue, and the heart. For apelin, whose possible effects on many bodily functions like the endocrine system, cardiovascular system and metabolic activities are still under investigation, the reproductive system is also an important target area. The purpose of the present study was to investigate the effects of plasma apelin levels in rats that were in diestrus, pregnancy and lactation periods, and to examine its possible effects on myometrium contractions of pregnant rats and non-pregnant rats that were in diestrus period...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213291/expression-profiles-of-proto-oncogene-twist1-and-tumor-metastasis-suppressor-gene-lass2-in-bladder-cancer
#9
Zeynep Yegin, Oguz Aydin, Haydar Koc, Recep Buyukalpelli
Transcription factor proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 have been reported to be involved in various carcinomas but their expression profiles and prognostic significances in bladder cancer are largely unknown. We aimed to determine these genes' expression levels both at mRNA and protein level in bladder cancer. mRNA expression levels of TWIST1 and LASS2 genes were examined using real-time quantitative PCR (qPCR) in human bladder tumors and paired normal adjacent tissues obtained from 44 patients...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213290/effect-of-hesperetin-on-inflammatory-and-oxidative-status-in-trinitrobenzene-sulfonic-acid-induced-experimental-colitis-model
#10
Fatin Rustu Polat, Ihsan Karaboga, Muhammed Semih Polat, Zeynep Erboga, Ahsen Yilmaz, Savas Güzel
In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213289/mir-637-suppresses-melanoma-progression-through-directly-targeting-p-rex2a-and-inhibiting-pten-akt-signaling-pathway
#11
Jian Zhang, Wen-Li Liu, Lu Zhang, Rui Ge, Fang He, Tian-Yuan Gao, Qiong Tian, Xin Mu, Li-Hong Chen, Wei Chen, Xu Li
MicroRNAs (miRNAs) play important roles in melanoma. Although miR-637 has been suggested to be a tumor suppressor in several cancers, its function in melanoma and the molecular mechanism behind that function remain unclear. In this study, we investigated the role of miR-637 in human melanoma and explored its relevant mechanisms. We found that the expression of miR-637 is significantly downregulated in melanoma tissues and cell lines. While overexpression of miR-637 inhibited melanoma cell proliferation and cell cycle G1-S transition, and induced apoptosis...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213288/investigation-of-cochlear-hair-cells-and-the-perception-of-ultrasound-signals-in-guinea-pigs
#12
Fu-Sen Wang, Guohui Nie, Cheng-Cheng Huang, Qian Li, Tiegang Li, Yuee Zou, Shaoyan Zhang, Xinyu Ceng
We established a specific ultrasound frequency-dependent model of cochlear injury using bone conduction ultrasounds in the inner ear of guinea pigs at 50 kHz and 83 kHz, to explore the effects of bone conduction ultrasound in the cochlea. To establish a unilateral cochlear damage model, the unilateral cochlea was destroyed. The control group consisted of 50 kHz and 83 kHz bone conduction ultrasounds in unaltered guinea pigs. In each group, cerebral blood oxygenation level dependent (BOLD) effects were determined by functional magnetic resonance imaging (fMRI)...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213287/screening-for-novel-peptides-specifically-binding-to-the-surface-of-ectopic-endometrium-cells-by-phage-display
#13
Yan Linzhi, Cao Dan, Samuel Kofi Arhin, Wang Ledan, Li Wenju, Lv Jieqiang
A 7-mer phage display library was employed to isolate novel peptides that specifically bind to ectopic endometrium in vitro. Phage display technology with biopanning and rapid analysis of selective interactive ligands between ectopic and eutopic endometrium cells was utilized. After 5 rounds of biopanning, 50 phage clones were randomly selected and analyzed by enzyme-linked immunosorbent assay and DNA sequencing. A peptide-competitive inhibition assay was performed to identify the affinity of positive phages toward ectopic endometrium cells...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213286/mir-33b-inhibits-osteosarcoma-cell-proliferation-through-suppression-of-glycolysis-by-targeting-lactate-dehydrogenase-a-ldha
#14
Xin-Min Zheng, Chun-Wei Xu, Fei Wang
Osteosarcoma (OS) is one of the most common types of malignant bone tumor in adolescent. MicroRNAs (miRNAs) are widely studied regulatory molecules which play important roles in tumor development, differentiation, growth, invasion, chemosensitivity and cellular metabolism. Recently, miR-33b has been reported to act as a tumor suppressor in osteosarcoma. However, the detailed mechanism of miR-33b in regulating osteosarcoma cell proliferation remains unclear. In this study, we detected miR-33b was significantly downregulated in osteosarcoma tissues compared to their matched adjacent nontumor tissues...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213285/lentiviral-mediated-bcl2-gene-knockdown-using-comparative-microrna-adaptive-shrnas
#15
Baharak Abdolhossein Zadeh, Kamal Yavari, Mehdi Banan, Ali Fallah, Leila Nasehi, Moloud Absalan, Gholamreza Tavoosidana
B-cell lymphoma 2 (BCL2) family proteins play a critical role in tuning cell death processes. Almost in half of all human cancers, a dysregulation in BCL2 family gene expression has been shown which made it an impressive target for human gene therapy as a novel approach in cancers. In this study we will optimize lentiviral-mediated RNA interference (RNAi), recombinant lentiviruses accommodating anti-BCL2 micro adaptive short hairpin RNAs (shRNAs), to downregulate BCL2 in human embryonic kidney 293T (HEK293T) cells to produce stable cell lines...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213284/genetic-variation-of-yellow-pistachio-hard-scale-lepidosaphes-pistaciae-archangelskaya-hem-diaspididae-populations-in-kerman-province-iran-revealed-by-issr-markers
#16
Mohammad Rouhani, Hassan Ali Vahedi, Alireza Marefat
Yellow pistachio hard scale, Lepidosaphes pistaciae (Hem.: Coccoidea: Diaspididae) is one of the detrimental pests to pistachio trees. This pest is distributed throughout the pistachio producing regions of Iran. It is complex species, having distinct genetic variation. As genetically diversity awareness is essential for identification and management, the diaspidid samples selected from 10 infected region and used to test hypotheses about the genetic variability between and within its populations, during 2016...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213283/determination-of-inhibitory-activities-of-enzymes-related-to-alzheimer-s-disease-and-diabetes-mellitus-of-plane-tree-platanus-orientalis-l-extracts-and-their-antioxidant-antimicrobial-and-anticancer-activities
#17
Esra Ucar, Nuraniye Eruygur, Mehmet Atas, Merve Ergul, Mustafa Ergul, Fazli Sozmen
Plane tree (Platanus orientalis L.) leaves have been employed for centuries in various countries due to their pharmacological value. Therefore, determination of the biological activity of the leaves is of interest. The aim of the study was to evaluate the inhibitory effects against Alzheimer's disease-related enzymes Acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α-amylase. The antioxidant, anticancer, and antimicrobial activities of the leaves were also studied...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213282/insecticidal-effects-of-the-olea-europaea-subsp-laperrinei-extracts-on-the-flour-pyralid-ephestia-kuehniella
#18
Souad Lahcene, Faiza Taibi, Nadjet Mestar, Samira Ali Ahmed, Mahieddine Boumendjel, Saida Ouafi, Karim Houali
In the present study, the aerial parts of the Laperrine olive (Olea europaea subsp. Laperrinei) are subjected to acid extraction and the chemical composition of the extracts is determined by HPLC-DAD. The main compounds found in all of extracts are: hydroxytyrosol (30.45%), tyrosol (0.69%), oleuropein (32.76%), ferrulic acid (17.77%), quercetin (31.57%) and hesperetin (6.90%).The extracts obtained from the leafy stems of Laperrine olive tree are tested on the moth Ephestia kuehniella flour. Their administration by inhalation of newly exuviated chrysalises extends the duration of nymphalid development and disturbs the exuviated adults reproduction, by reducing the period in which the eggs are being laid...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30213281/the-effect-of-6-week-zinc-supplement-and-weight-training-on-the-blood-lipids-of-the-sedentaries-and-athletes
#19
Vedat Cinar, Taner Akbulut, Yakup Kilic, Mustafa Özdal, Mucahit Sarikaya
In this study, we aimed to investigate the effect of 6 weeks of zinc supplementation and weight training on blood lipids of sedentary and athletes. Research group consists of total 40 males; 20 athletes who do regularly physical exercises and 20 sedentary volunteers. The volunteers were divided into four groups. These groups were constituted in that way: the first group is a natural sedentary group which called control group as well (S); the second group is another sedentary group which is supplied with only zinc (Z+S); the third group is training group which composing of athletes and is supplied with zinc (Z+T); the fourth group is the natural training group or athletes (T)...
August 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30084812/meta-analysis-of-gene-expression-profiles-of-peripheral-blood-cells-in-systemic-lupus-erythematosus
#20
Sang-Cheol Bae, Young Ho Lee
Our purpose was to identify differentially expressed (DE) genes and biological processes associated with gene expression changes in systemic lupus erythematosus (SLE). We performed a meta-analysis using the INMEX program (integrative meta-analysis of expression data) on publicly available microarray Genetic Expression Omnibus (GEO) datasets of peripheral blood mononuclear cells (PBMCs) of SLE patients and healthy controls. We performed Gene Ontology (GO) enrichment analysis by using hypergeometric tests. In total, five comparisons (2 B cells, 2 CD4 T cells, and 1 myeloid cell) from two GEO datasets containing 51 cases and 46 controls were included in the meta-analysis...
July 30, 2018: Cellular and Molecular Biology
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