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Cellular and Molecular Biology

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https://www.readbyqxmd.com/read/29433629/effect-of-decapitation-and-exogenous-application-of-gibberellic-acid-ga3-and-cytokinin-ck-on-some-physiological-characteristics-of-stevia
#1
Mehrangiz Akbari, Mohammad Eghbal Ghobadi, Mokhtar Ghobadi, Said Jalali-Honarmand, Mohsen Saeidi
Stevia (Stevia rebaudiana B.) has auxiliary buds that often remain dormant for a long time and sometimes remain dormant until the plants change at the reproductive stage. This study was designed out to investigate whether decapitation and exogenous application of plant growth regulators enhance the productivity of stevia through breaking the apical dominance and increasing physiological characteristics. Experiment was carried out as a factorial in randomized complete block design with three replications. Factors were consisted two agricultural practices (Decapitation and No-decapitation) and eight foliar spray including without spray as control, water spray, GA3 (300, 600 and 900 µm) and CK (100, 200 and 400 µm)...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433628/callus-induction-and-somatic-embryogenesis-in-stevia-rebaudiana-bertoni-as-a-medicinal-plant
#2
Tahereh Keshvari, Abdollah Najaphy, Danial Kahrizi, Alireza Zebarjadi
Stevia rebaudiana (Bert.) from Asteraceae family is a useful medicinal plant that prevents and cures diabetes, blood pressure, weight gain and tooth decay. Due to self-incompatibility in stevia, somatic embryo investigation for artificial seed production is valuable in this plant. In order to evaluate the callus induction characteristics in stevia, a factorial experiment was laid out based on a completely randomized design with three replications. The factors included ten hormone combinations and control, two kinds of media (MS and B5) and two types of explants (leaf and internode)...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433627/the-effects-of-different-nitrogen-doses-on-antioxidant-and-antimicrobial-activity-of-stevia-stevia-rebaudiana-bert
#3
Mehmet Atas, Nuraniye Eruygur, Esra Ucar, Yasar Ozyigit, Kenan Turgut
In this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha-1) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433626/metal-oxides-as-a-biostimulator-for-upregulation-of-genes-involved-in-the-biosynthesis-of-rebaudioside-a
#4
Alireza Mirzaei, Gholamreza Kavoosi, Sara Dezhsetan, Mahdi Behnamian
Stevia rebaudiana Bertoni is a kind of perennial medicinal plant with sweetening properties which belongs to Asteraceae family. Its leaves with fundamental glycoside compounds consist of both a sugar part and a non-sugar sector. One of the glycoside compounds is Rebaudioside- A which has a greater importance in business. This experiment was conducted to evaluate the effects of Ag2O, CrO3, PbO, Fe2O3, BaO and TiO2 on the expression pattern of these genes in the Stevia rebaudiana. Rebaudioside- A biosynthesis was repeated 3 times with concentrations of 50, 100 and 200µM...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433625/effects-of-different-concentrations-of-mannitol-on-gene-expression-in-stevia-rebaudiana-bertoni
#5
Somaye Hashempoor, Matin Ghaheri, Danial Kahrizi, Nastaran Kazemi, Saare Muhammadi, Seyed Mehdi Safavi, Tayebeh Ghorbani, Elham Rahmanian, Malihe Heshmatpanaah
Stevia rebaudiana Bertoni is one of the most important herbal sweetener plants from Asteracea family that have a lot of Steviol glycosides. Among different methods, tissue culture is the best way with high efficiency that is useful for studying stress tolerance mechanisms to obtain drought tolerance of stevia. For this purpose, different concentrations of mannitol (0, 10, 20, 30, 40, 50 mg/l) were used as various treatments in the culture medium of stevia. According to the results, the highest level of UGT85C2 gene expression (1...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433624/investigation-of-different-concentrations-of-ms-media-effects-on-gene-expression-and-steviol-glycosides-accumulation-in-stevia-rebaudiana-bertoni
#6
Danial Kahrizi, Matin Ghaheri, Zahra Yari, Khirollah Yari, Sohbat Bahraminejad
Stevia rebaudiana Bertoni is one of two species that contains steviol glycosides. Among steviol glycosides that extracted from leaves, stevioside and rebaudioside A are the two major and the sweetest glycosides that are about 200-300 times sweeter than sucrose with zero calories. The best method for stevia propagation is tissue culture. So, for investigation of nutrients in medium, we studied the effect of different concentrations of MS media (MS, 0.5 MS, 0.25 MS, 0 MS) on morphological traits, UGT74G1 and UGT76G1 genes expression and accumulation of steviol glycosides in stevia leaves...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433623/effects-of-life-cycle-and-leaves-location-on-gene-expression-and-glycoside-biosynthesis-pathway-in-stevia-rebaudiana-bertoni
#7
Matin Ghaheri, Elaheh Adibrad, Seyed Mehdi Safavi, Danial Kahrizi, Ali Soroush, Saare Muhammadi, Tayebeh Ghorbani, Ali Sabzevari, Zahra Ansarypour, Elham Rahmanian
Stevia rebaudiana Bertoni is One of the most important biologically sourced and low-calorie sweeteners that known as "Sweet Weed". It contains steviol glycosides that they are about 200-300 times sweeter than sucrose. Tissue culture is the best method with high efficiency that can overcome to problems of traditional methods, and it is the most useful tools for studying stress tolerance mechanisms under in vitro conditions to obtain drought tolerance. In the present research, we investigated the impact of life cycle, leaves location and the harvesting time on expression of UGT74G1 and UGT76G1 as well as steviol glycosides accumulation...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433622/effect-of-nitrogen-sources-on-gene-expression-of-stevia-rebaudiana-bertoni-under-in-vitro-conditions
#8
Fariba Akbari, Ali Arminian, Danial Kahrizi, Arash Fazeli, Matin Ghaheri
Stevia rebaudiana (Bertoni) is a non-caloric sweetener belonging to Asteraceae family. Stevia compounds such as steviol glycosides (SGs) are 200 times sweeter than sugar. Stevioside and rebaudioside A are the two major steviol glycosides. Nitrogen is an essential element for plant growth and development. In this study the effects of nitrogen influenced by different concentrations of NH4NO3 (0, 825 and 1650 mg/l) and KNO3 (0, 950 and 1900 mg/l) is examined in MS medium. To analysis the UGT74G1 and UGT76G1 genes expression, involved in the synthesis of SGs, RT-qPCR technique was performed...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433621/effect-of-stevia-rebaudiana-bertoni-extract-on-sexual-dysfunction-in-streptozotocin-induced-diabetic-male-rats
#9
Matin Ghaheri, Shahram Miraghaee, Atefeh Babaei, Bahareh Mohammadi, Danial Kahrizi, Zahra Minoosh Saivosh Haghighi, Gholamreza Bahrami
Stevia rebaudiana Bertoni has been used locally as a non-calorie sweetener in medicine and diabetic diet which claimed to have aphrodisiac properties, although no scientific data of this function have been reported. The aim of this study was to investigate the effect of S. rebaudiana extract on sexual dysfunction, testosterone levels and number of Leydig cells in Streptozotocin (STZ)-induced diabetic male rats. A total of 28 diabetic male rats were randomly divided into 4 groups: diabetic group without any extract and 3 extract groups (5, 50 and 100 mg/kg)...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29433620/effects-of-various-glutamine-concentrations-on-gene-expression-and-steviol-glycosides-accumulation-in-stevia-rebaudiana-bertoni
#10
Fatemeh Esmaeili, Matin Ghaheri, Danial Kahrizi, Mohsen Mansouri, Seyed Mehdi Safavi, Tayebeh Ghorbani, Sarre Muhammadi, Elham Rahmanian, Siavash Vaziri
Stevia rebaudiana Bertoni is one of the most important biologically sourced and low-calorie sweeteners that contains a lots of Steviol glycosides. Tissue culture is the best method for propagation of stevia and micro nutrients can affect both morphological traits and steviol glycosides production. In the present study, we investigated the effect of different concentrations of glutamine (10, 20, 30 and 40 g/l) on expression of UGT74G1 and UGT76G1 genes and stevioside and rebaudioside A accumulation in the leaves of stevia under in vitro conditions...
February 10, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412808/circulating-prolactin-levels-and-behcet-s-disease-a-meta-analysis
#11
Gwan Gyu Song, Young Ho Lee
This study aimed to summarize the current evidence on the relationship between circulating prolactin levels and Behcet's disease (BD). We performed a meta-analysis comparing the serum/plasma prolactin levels in patients with BD with those of controls and performed a subgroup analysis based on ethnicity, disease activity, and sex. Ten articles with a total of 320 patients with BD and 259 controls were included. The prolactin levels were not significantly higher in the BD group than in the control group (SMD=0...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412807/association-between-vitamin-d-level-and-or-deficiency-and-systemic-lupus-erythematosus-a-meta-analysis
#12
Sang-Cheol Bae, Young Ho Lee
This study aimed to evaluate the relationship between the level of 25-hydroxyvitamin D [25(OH)D] and systemic lupus erythematosus (SLE). We searched the PUBMED, EMBASE, and Cochrane databases and performed a meta-analysis examining the vitamin D level and/or deficiency in patients with SLE, compared with that in healthy controls. In total, 18 studies consisting of 1,083 patients with SLE and 1,273 controls were included. Vitamin D level was significantly lower in the SLE group than in the control group (standardized mean difference [SMD] = -0...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412806/ethanol-promotes-apoptosis-in-rat-ovarian-granulosa-cells-via-the-bcl-2-family-dependent-intrinsic-apoptotic-pathway
#13
Yu Liu, Haojiang Zuo, Yao Wang, Liantian Tian, Xin Xu, Jingyuan Xiong, Xiaofang Pei
Excessive and indulgent alcohol consumption causes tremendous public health issues worldwide. Not only is ethanol associated with a broad spectrum of critical chronic diseases, ethanol is also demonstrated to exert striking reproductive toxicity for both males and females. Epidemiological investigations suggested that ethanol is closely related to fertility decrease in women. Animal studies showed that ethanol intake obstructed ovulation and reduced ovarian weight during gestation. However, cellular mechanism for this inhibitory effect of ethanol on female fertility is yet to be explored...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412805/molecular-mechanisms-underlying-the-participation-of-ribonuclease-t2-gene-into-self-incompatibility-of-citrus-grandis-var-shatianyu-hort
#14
Xin Min Qin, Yu Zhang, Yu Jie Liu, Dan Ni Guo, Hui Min Li
The RIB (ribonuclease T2) of Citrus grandis var. Shatianyu Hort involved in self-incompatibility (SI) mechanism was identified by prokaryotic expression. RT-qPCR results showed that the expression level of RIB in self pollinated stigma is significantly higher than that in cross pollinated stigma. A vector for prokaryotic expression of RIB was constructed after codon-optimization, and the recombinant protein was induced and purified. In vitro pollen germination test indicated that the RIB protein markedly inhibited pollen germination and pollen tube growth...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412804/testosterone-to-estrogen-conversion-is-not-responsible-for-the-vasodilating-effects-of-testosterone-ex-vivo
#15
Shijin Tan, Dong Yi, Wei Zhu, Rong Sun, Meng Wei
Men have a higher incidence of cardiovascular disease, but poorer vasodilatation than women. However, testosterone exerts vasodilating actions ex vivo. We aimed to determine if reactive oxygen species (ROS) produced in vivo could cause 'eNOS uncoupling' that accounts for the disparity between in vivo and ex vivo results. Ovariectomized SHR and WKY rats were divided into 3 groups: untreated, estradiol benzoate (EB) treated, and testosterone propionate (TP) treated. EB and TP rats were treated for 8 weeks, and blood pressure, serum estrogen, progesterone, and testosterone were measured...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412803/high-temperature-requirement-factor-a1-htra1-regulates-the-activation-of-latent-tgf-%C3%AE-1-in-keloid-fibroblasts
#16
Shi-Feng Jin, Yu-Xin Wang, Nan Xu, Qiang Sun, Chen-Chao Wang, Meng-Zhu Lv, Xu Sun, Shu Guo
Scar treatments are considered a major issue in the plastic surgery field. Activation of the transforming growth factor-β (TGF-β)-mediated signaling pathway plays a key role in the scar pathogeneses, and high temperature requirement factor A1 (HTRA1) inhibits TGF-β1 activation in tumor cells. Our study aims to investigate the role of HTRA1 in the pathogenesis of scars. The mRNA levels of HTRA1 was evaluated by real time PCR, HTRA1 protein expression was determined using western blot and immunohistochemistry, and a luciferase assay was applied to measure dynamic changes of TGF-β1 activity...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412802/high-fat-diet-stimulates-the-gut-pathogenic-microbiota-and-maintains-hepatic-injury-in-antibiotic-treated-rats
#17
Sooad Al-Daihan, Abir Ben Bacha, Abeer M Al-Dbass, Mona Awad Alonazi, Ramesa Shafi Bhat
The gut and the liver are closely linked to each other, as changes in the gut microbiota can play a significant role in the development of many liver diseases. Gut bacteria respond rapidly to changes in diet and thus can affect the liver through their metabolites. The impact of a high lipid diet on the liver in the presence of an altered gut flora modulated by ampicillin was investigated. The study was performed on 30 male Western albino rats randomly divided into 3 groups: control (phosphate buffered saline treated), group II (ampicillin 50 mg/kg for three weeks to induce microbiota alterations and fed on standard diet) and group III (same dose of ampicillin and fed on a lipid rich diet)...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412801/differentially-expressed-secretoglobin-1c1-gene-in-nasal-polyposis
#18
Sibel Özdaş, Talih Özdaş
Nasal polyps (NP) are the most common pathological change that occurs in the nasal mucosa and is characterized by mucosal inflammation. Although its etiology and pathogenesis have not been clearly explained, its pathophysiology is arranged by the balance between pro-inflammatory and anti-inflammatory cytokines. The Secretoglobin 1C1 gene synthesizes odor molecule binding proteins (OBPs) in the nasal mucosa and regulates some cytokines. The Secretoglobin 1C1 gene expression could be disrupted by polymorphisms that may act as a possible cause of a disruption in the regulation of the promotor of the gene...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412800/organ-transplantation-and-donation-from-the-point-of-view-of-medical-students-in-iran-ethical-aspects-and-knowledge
#19
Mahmoud Abbasi, Mehrzad Kiani, Mehdi Ahmadi, Bahare Salehi
Organ transplantation is an effective process that prolongs the lives of individuals suffering from incapacitating conditions. The aim of this study was to evaluate the view point of medical students in Iran regarding ethical aspects and knowledge on organ transplantation and donation. The participants included 165 medical students from different faculties of Shahid Beheshti University of Medical Sciences, Tehran, Iran. They were assessed using a reliable questionnaire that examined their ethical aspects and knowledge regarding organ transplantation and donation...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29412799/correlation-between-serum-leptin-and-its-gene-expression-to-the-anthropometric-measures-in-overweight-and-obese-children
#20
Maha K Eldosouky, Azza M Abdu Allah, Abeer AbdElmoneim, Najat Saaeid Al-Ahmadi
Obesity is a multifactor disorder with evidence supporting the role of the genetic factor in its etiology. The present study evaluates the relationship between leptin G2548A (rs7799039) and leptin receptors (Gln223Arg (rs1137101) genotyping and its leptin level and the risk of childhood obesity. This case-control study was conducted on 168 overweight and obese Saudi children and 126 non obese one served as control. Fasting insulin, leptin, blood glucose, lipid profile levels were measured. HOMA- IR, and BMI were assessed...
January 31, 2018: Cellular and Molecular Biology
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