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Cellular and Molecular Biology

Sang-Cheol Bae, Young Ho Lee
This study explored whether the functional protein tyrosine phosphatase nonreceptor 22 (PTPN22) G788A (R263Q) polymorphism is associated with susceptibility to autoimmune diseases. A meta-analysis was conducted using 23 comparative studies with a total of 16,719 patients and 17,783 controls. The meta-analysis showed an association between the A allele of the PTPN22 G788A polymorphism and decreased risk of autoimmune diseases in all subjects (p < 0.001). Analysis after stratification by ethnicity indicated that the PTPN22 788A allele was significantly associated with autoimmune diseases in Europeans (p < 0...
April 30, 2018: Cellular and Molecular Biology
Guanglei Fu, Lijun Fu, Youli Cai, Hui Zhao, Wenjun Fu
Recent studies have evaluated the associations between polymorphisms of glucocorticoid receptor genes and asthma. However, the conclusions of these studies are conflicting. The objective of this meta-analysis was to clarify the association between all known polymorphisms of glucocorticoid receptor genetic loci and susceptibility to asthma, based on existing reports. We conducted a meta-analysis of the association between glucocorticoid receptor polymorphisms (NR3C1) and asthma risk. A systematical literature search was performed in PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure (CNKI), and Cochrane Library until January 15, 2018...
April 30, 2018: Cellular and Molecular Biology
Sajad Moradi, Salar Khaledian, Mohadese Abdoli, Mohsen Shahlaei, Danial Kahrizi
Detection and quantification of various biological and non-biological species today is one of the most important pillars of all experimental sciences, especially sciences related to human health. This may apply to a chemical in the factory wastewater or to identify a cancer cell in a person's body, it may be apply to trace a useful industrial microorganism or human or plant pathogenic microorganisms. In this regard, scientists from various sciences have always striven to design and provide tools and techniques for identifying and quantifying as accurately as possible to trace various analyte types with greater precision and specificity...
April 30, 2018: Cellular and Molecular Biology
Liu Shuaichen, Wang Guangyi
Cancer cell lines are useful tools to study cancer biology. Choosing proper cell lines based on experimental design for different experiments is vital. Relating tumors and cell lines, and recognizing their similarities and differences are thus very important for translational research. Abundant online databases with genomic and expression profile are suitable resources for conducting the assessment. Pancreatic ductal adenocarcinoma (PDAC) is a severe cancer with grim prognosis. Current effective treatments of PDAC remain limited...
April 30, 2018: Cellular and Molecular Biology
Azza S El-Demerdash, Mohamed G Aggour, Mona M El-Azzouny, Sally H Abou-Khadra
The study investigated 110 Enterobacteriaceae isolates from broiler chickens isolated from Sharkia poultry farms and analyzed the isolates antimicrobial resistance and the presence of integrons as a potential basis for this resistance. Antibiotic susceptibilities against 12 different antibiotics were determined by the disk diffusion method. Prevalences and classes of integrons were then detected in multi-drug resistant (MDR) strains using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by sequencing of the variable parts...
April 30, 2018: Cellular and Molecular Biology
Monica Cecati, Alessandra Corradetti, Davide Sartini, Valentina Pozzi, Stefano R Giannubilo, Franca Saccucci, Andrea Ciavattini, Monica Emanuelli
Pelvic organ prolapse (POP) is a common disorder in women. It is characterized by the descent of the vaginal wall with consequent drop of pelvic organs. Pregnancy, labour and childbirth seem to be important events leading to the development of POP, since they are associated with prolonged stretch and mechanical stress of muscles, ligaments and connective tissue supporting pelvic organs. In pubocervical fascia, we explored the expression level of extracellular matrix and adhesion molecules. Tissue samples were obtained from twenty patients with POP who underwent cystocele repair, and from twenty control subjects during hysterectomy surgery...
April 30, 2018: Cellular and Molecular Biology
Xiang Zheng, Shushu Wang, Dakai Chen, Xiaoming Yang
Swainsonine is an Astragalus membranaceus extract. It is indole, alkaloid, and soluble in water. Its effect on rat cardiomyocytes apoptosis, and the mechanisms underlying that effect, were investigated by inducing apoptosis in H9c2 cells. This was detected by MTT assay, Annexin V-FITC/propidium iodide double staining and western blotting. Flow cytometry and fluorescence microscopy were used to confirm swainsonine's effect on mitochondrial membrane potential and levels of reactive oxygen species, while an ATP-dependent bioluminescence assay kit served to find the ATP contents...
April 30, 2018: Cellular and Molecular Biology
Tarunkumar Hemraj Madne, Mark Edward Carl Dockrell
Alternative splicing is an important gene regulation process to distribute proteins in health and diseases. Extra Domain A+ Fibronectin (EDA+Fn) is an alternatively spliced form of fibronectin (Fn) protein, present in the extra cellular matrix (ECM) and a recognised marker of various pathologies. TGFβ1 has been shown to induce alternative splicing of EDA+Fn in many cell types. Podocytes are spectacular cell type and play a key role in filtration and synthesise ECM proteins in renal physiology and pathology...
April 30, 2018: Cellular and Molecular Biology
Manal Mohamed Elhassan Taha, Hisham Elraih Homeida, Omer Mohammed Elhassan Dafalla, Siddig Ibrahim Abdelwahab
The present study was conducted to evaluate the susceptibility, multidrug resistance and genetic characteristics of Escherichia coli (E. coli) strains, isolated from clinical samples from Jazan Hospital, Jazan, Saudi Arabia. A total of 95 samples were recruited from wound, urine, stool and blood. The isolates were assessed for their antibiotic susceptibilities and the presence of class II and III integrons was studied using PCR technique. Integron II-positive PCR products were further confirmed using partial sequencing...
April 30, 2018: Cellular and Molecular Biology
Shahrzad Soleimani, Shohreh Alizadeh Shargh, Aliasghar Keramatinia, Narjes Mehrvar, Neda Mansouri, Abbas Doosti, Seyed Abdolreza Mortazavi-Tabatabaei, Mehrdad Hashemi, Abolfazl Movafagh
Sentinel lymph node (SLN) micrometstasis detection improves outcome for breast cancer follow up procedure. The aim of the present study was to identify gene profiles that accurately predicted the outcome of breast cancer patients. Fifty tumor sample from breast cancer patients were analyzed for the expression of 3 genes using quantitative-PCR. Also clinical verification for recurrence to distant organs was performed. Three gene signature were confirmed based on tumor's stage, grade, ER status, using conditional logistic regression...
April 30, 2018: Cellular and Molecular Biology
Lai-Quan Huang, Jian-Xin Wang, Kun He, Yi-Zhi Jiang, Zhong-Ling Wei, Dong-Ping Huang, Li-Li Chu
To study the peripheral blood T-cell subsets and regulatory T-cells of multiple myeloma (MM) patients. 48 MM patients and 24 healthy controls were enrolled. Changes in peripheral blood T-cell subsets in the MM patients i.e. CD4+CD25+T cells and CD4+CD25+CD127lowT regulatory cells (CD4+CD25+CD127lowTregs) and in healthy controls were measured using flow cytometry and immunohischemistry. The total T-cells (CD3+) in peripheral blood lymphocyte and auxiliary/induced T-cells (CD3+CD4+ T cell) of the 48 MM patients showed no statistical significance when compared with those of the control group...
April 30, 2018: Cellular and Molecular Biology
Qiong-Dan Hu, Wei-Hua Wu, Yan Zeng, Ji Wen, Xiao-Jun Li, Wei Pan, Mao-Ping Zhang, Bo Hu, Chun-Yan Lei, Junming Fan
In recent years, metabolomics using high-performance liquid chromatography (UPLC) has been used to study the metabolic profiles in plasma, urine, stool and tissue in animal model of chronic kidney disease (CKD). In the previous work, we found that traditional Chinese medicine (TCM) "Kidney Flaccidity Compound" (KFC) based on "kidney flaccidity theory" can improve renal function and quality of life of patients with kidney disease. This study aimed to investigate the metabolic profiles in peripheral blood of hemodialysis patients administrated by KFC for 1...
April 30, 2018: Cellular and Molecular Biology
Neslihan Turgut Kara, Özgür Çakır, Burcu Arıkan, Şule Arı
Phenylalanine ammonia lyase (PAL) is the first enzyme of the phenylpropanoid pathway, and it is necessary to upregulate flavonoid biosynthesis in most of the plant species. In this study, we have cloned PAL gene from endemic Astragalus chrysochlorus which is a producer of phenolic nicotiflorin (kaempferol-3-O-rutinoside). The cDNA encoding PAL was cloned from A. chrysochlorus using RT-PCR (reverse transcription-polymerase chain reaction) with conserved primer pairs. Amino acid sequence alignments showed that AcPAL (2160 bp, Accession number: KM189182) has more than 95% amino acid identity with their homologues in other Astragalus species...
April 30, 2018: Cellular and Molecular Biology
Mohammad Ghozali, Tiwi Harjanti Cakranita, Adi Imam Tjahjadi, Lelani Reniarti, Reni Ghrahani, Mraa Syamsunarno, Budi Setiabudiawan, Ramdan Panigoro
Lifetime blood transfusion experienced by major β-thalassemia patients complicated with iron overload, therefore, may lead to their tissue injury. Ultimately, free toxic iron may alter immune response via dysregulation of immune cell activity producing prolonged effector reaction. Neutrophil as one of the vital innate immune cell despite serves as the first line of defense resulting acute inflammation has a pivotal role in chronic inflammation while releasing the toxic substance that interferes biological processes...
April 30, 2018: Cellular and Molecular Biology
Zahra Raziei, Danial Kahrizi, Hosein Rostami-Ahmadvandi
Due to the importance of Camelina for low expectation (water and other inputs) and as an oil crop, Soheil cultivar was cultivated in Ardebil, Hamedan, Rasht, Ilam, Kermanshah, Karaj, Mashhad, Ahvaz and Bushehr Provinces. Fatty acids were measured with MG-Mass. Results showed that morphological traits were not very dependent on the climate, but the profile of the fatty acids was dependent. ANOVA of the effects of climate on the saturated fatty acid showed that there were significant differences between climates for all studied SFAs (P<0...
April 30, 2018: Cellular and Molecular Biology
HyunKyung Lee, Hwa Jun Cha
In skin, melanocytes determine skin color using melanogenesis, which induces protective mechanism to oxidative stress and UV damage. However, when melanin is excessive produced by the various stimulus, the accumulated melanin induces hyperpigmentation disease such as melasma, freckles, Melanism ware induced. Therefore, it is implicated to finding potential agents for whitening to be used in cosmetic products. In our present study, we show that Poria cocos Wolf extracts decreased melanin synthesis in B16F10...
April 30, 2018: Cellular and Molecular Biology
Min Zhang, Qiong Li, Lu Zhang, Yunjian Wang, Linxia Wang, Qingjun Li, Tao He, Baishun Wan, Xiaoqian Wang
The Ras-association domain family (RASSF) proteins have been involved in many important biological processes. RASSF7 is recently reported to be up-regulated in several types of cancer. However, the function of RASSF7 remain unknown in human cancers. To explore the role of RASSF7 in hepatocellular carcinoma (HCC) cells proliferation and molecular mechanism. RASSF7 expression was examined using public database TCGA, qRT-PCR and Western blot. The correlation between RASSF7 and clinicopathological features was measured...
April 30, 2018: Cellular and Molecular Biology
Sibel Özdaş
The effect of deregulation of nuclear export mediated by exportin-1, with consequent cellular mislocalization of p33ING1b, a member of the tumor suppressor gene family, has not been previously investigated in head and neck squamous cell cancer (HNSCC). We evaluated the effect of reversing cytoplasmic p33ING1b localization through inhibition of exportin-1 by leptomycin B (LMB) and the effect of nuclear entrapment of p33ING1b on molecular alterations in primary and metastatic HNSCC lines. The expression and location of exportin-1 and p33ING1b were analyzed by a quantitative real‑time reverse transcription polymerase chain reaction PCR (qRT-PCR), a Western blot, and immunostaining...
April 30, 2018: Cellular and Molecular Biology
Mustafa Cengiz
The goal of this study was to determine the effects of  boric acid (B) as a boron source on blood cells and indirectly on bone marrow. Intraperitoneally (i.p.) administration of 200 mg / kg of cyclophosphamide (CP) resulted in reductions in the number of erythrocyte (20%), hemoglobin (20%), leukocytes (96%), thrombocytes (41%), and hematocrit (21%). The group given CP alone was killed 3 days after the CP administrated. For the group having CP+B (200 mg/kg i.p) treatment was started 3 days earlier than the CP administration and continued to the finish of the experiment (6 days)...
April 30, 2018: Cellular and Molecular Biology
Roheena Abdullah, Bakhtawar Javed Peracha, Kinza Nisar, Mehwish Iqtedar, Afshan Kaleem, Tehreema Iftikhar, Faiza Saleem, Shagufta Naz
Endoglucanase is one of the most important enzymes of the cellulase group.  Endoglucanase are involved in the catalytic hydrolysis of cellulose and plays a pivotal role in different sectors like pharmaceutical, textile, detergent, and food processing as well as paper and pulp industry. With consumers getting more and more aware of environmental issues, industries find enzymes as a better option over other chemical catalysts. In the current research different thermophilic fungal strains were isolated from the different sources...
April 30, 2018: Cellular and Molecular Biology
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