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Cellular and Molecular Biology

Y-L Qu, C-M Wu, L-X Zhang, B-L Wen, X Zhang, C Ma, X-Y Yan, H-B Cui, D-M Wang, Y-R Ji
Some studies investigated the association between ADD1 rs4963 polymorphism and hypertension risk. However, the results remained inconclusive. Thus, we performed a meta-analysis. Published reports were searched in PubMed and Google Scholar. The strength of association was assessed by calculating odds ratios (OR) and 95% confidence interval (CI). Twelve studies with 5097 cases and 5937 controls were conducted in this study. Individuals with ADD1 rs4963 polymorphism showed an increased hypertension risk (OR = 1...
November 30, 2016: Cellular and Molecular Biology
B Hajebrahimi, A Kiamanesh, A A Asgharnejad Farid, G Asadikaram
Mental disorders including depression and anxiety are the prevalent human diseases which are associated with several metabolic and non-metabolic diseases. Recent investigations demonstrated that the mental disorders can be considered as the potential inducers/stimulators of chronic inflammation. Based on the fact that chronic inflammation is a main cause of human diseases, hence, a hypothesis has been raised to explore the interaction between mental disorders and inflammation related metabolic diseases. Type 2 diabetes (T2D), as a complicated metabolic disorder, is associated with inflammation and also mental disorders...
November 30, 2016: Cellular and Molecular Biology
J Li, W Yi, P Jiang, R Sun, T Li
Our previous preliminary study revealed a synergistic effect of ambroxol hydrochloride with chemotherapeutic agents such as paclitaxel and carboplatin in lung cancer. However, the optimal conditions such as administration time and drug concentration of ambroxol hydrochloride to achieve the maximum synergistic effect remained unclear. Therefore, concentration changes of the chemotherapy drugs paclitaxel and carboplatin in the sputum were observed after ambroxol hydrochloride administration at different times in order to determine the most effective time frame of ambroxol hydrochloride administration...
November 30, 2016: Cellular and Molecular Biology
D Kirac, F Eraydin, T Avcilar, K Ulucan, F Özdemir, A I Guney, E Ç Kaspar, E Keshi, T Isbir
ooth agenesis, affecting up to 20% of human population, is one of the most common congenital disorder. The most frequent form of tooth agenesis is known as hypodontia, which is characterized by the absence of one to five permanent teeth excluding third molars. It was considered that hypodontia is especially related with gene mutations which play role in tooth formation. Additionally mutations in PAX9 and/or MSX1 have been identified as the defects responsible for missing permanent molars and second premolars...
November 30, 2016: Cellular and Molecular Biology
H-F Wang, L Chen, J Luo, H-X He
The interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. Thus, the present study aimed to identification novel regulation factors of IFITM genes. Our data showed up-regulation of IFITM1, IFITM2, and IFITM3 genes in A549 by H5N1 virus infection. However, IFITM1 was not affected, IFITM2 and IFITM3 increases expression following different concentration H5N1 virus infection in HEK293T cells. This is the first time to find differences of IFITM1 transcription in different cells with the H5N1 virus infection...
November 30, 2016: Cellular and Molecular Biology
L Chen, E Liu, D Cao, S Li, C Xiao, M Xiong, Q Kou
The present study investigated the relationship between uncoupling of endothelial nitric oxide synthase (eNOS) and vascular endothelial cell (VEC) oxidative stress (OS) during sepsis and the role of eNOS glutathionylation in eNOS uncoupling of septic VECs. Human umbilical vein endothelial cells (HUVECs) cultured in vitro (EA.hy269 cell line) were incubated with Dulbecco's modified Eagle's medium (DMEM) (normal control group), lipopolysaccharide (LPS) (sepsis group), 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) (glutathionylation group), and LPS+ dithiothreitol (DTT) (deglutathionylation sepsis group)...
November 30, 2016: Cellular and Molecular Biology
M E Yonar, K Koprucu
In this study, it was examined the effects of dietary omega-3 fatty acids on the haematocrit (Ht), haemoglobin (Hb), mean corpuscular haemoglobin (MCH), total protein (TP) and total immunoglobulin (TI) values, erythrocyte (RBC) and leucocyte (WBC) counts, mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), phagocytic index (PI) and nitroblue tetrazolium (NBT) activity in rainbow trout (Oncorhynchus mykiss) milked regularly. For this aim, one control and two experimental diets were prepared as isonitrogenous and isocaloric...
November 30, 2016: Cellular and Molecular Biology
W-D Wang, Y-E Sun
1% of tea polyphenol, chitosan solution and potassium sorbate were used as film-forming materials to coat chilled mutton. Total coliforms, TVB-N value and pH value were determined and used as the mutton fresh-keeping indexes. The results showed that after 12th day at the end of the storage, mutton coated with tea polyphenol had best effects comparing chitosan solution and potassium sorbate. pH value of mutton coated by tea polyphenol was 6.0, TVB-N and the total coliforms were both significantly lower than the meat coated by chitosan solution and potassium sorbate...
November 30, 2016: Cellular and Molecular Biology
O Barim-Oz, H Şahin
This study was conducted to determine the effect of starvation (78 days) and refeeding (33 days) on oxidative stress (malondialdehyde (MDA)),  antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px)), arginase (AR) and nitric oxide (NO) in the hepatopancreas, muscle and gills tissues of freshwater crayfish (Astacus leptodactylus). Crayfish were maintained at three experimental groups: control (fed), starved (not fed) crayfish for 78 days and refeeding crayfish for 33 days after 78 days starvation...
November 30, 2016: Cellular and Molecular Biology
W Song, W H Zhang, H Zhang, Y Li, Y Zhang, W Yin, Q Yang
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become a frequently used strategy in gene expression studies. The relative quantification method is an important and commonly used method for the evaluation of RT-qPCR data. The key of this method is to identify an applicable internal control gene because the usage of different internal control genes may lead to distinct conclusions. Herein, we report the validation of 12 common housekeeping genes for RT-qPCR for gene expression analysis in the Oral squamous cell carcinoma (OSCC) cell line (KB and Tca-8113) treated by 5 kinds of Chemotherapy Drugs...
November 30, 2016: Cellular and Molecular Biology
A El-Seedy, M C Pasquet, H Shafiek, T Morsi, A Kitzis, V Ladevèze
Cystic fibrosis (CF) occurrence in Arab populations is not common and still remains underidentified. Furthermore, the lack of disease awareness and diagnosis facilities have mislead the identification of cystic fibrosis for decades. The knowledge about cystic fibrosis (CF) in Egypt is very limited, and a few reports have drawn attention to the existence of CF or CFTR-related disorders (CFTR-RDs) in the Egyptian population. Therefore a comprehensive genetic analysis of the CFTR gene was realized in patients of North Egypt...
November 30, 2016: Cellular and Molecular Biology
R Ghiasi, M Mohammadi, M Majidinia, B Yousefi, A Ghiasi, R Badalzadeh
Reperfusion of the heart after an ischemic insult may lead to potentially lethal arrhythmias and cardimyocyte cell death via apoptosis and necrosis. In addition, previous studies showed that calcium channel blockers may have a protective role in the myocardium against arrhythmia and irreversible tissue injury. Therefore, this study was aimed to investigate the effects of mebudipine on myocardial arrhythmias and tissue injury induced by ischemia/reperfusion injury in isolated rat hearts. Male Wistar rats (250‑300g) were randomly divided to Sham group (without ischemia), control group (ischemia without drug), drug group (ischemia with mebudipine 0...
November 30, 2016: Cellular and Molecular Biology
B-X Li, X-F Cao, C-Y Weng, X-S Fang, H-B Mao, M-M Guan, G-L Liu
To investigate the effects of autophagy activator and autophagy inhibitor on the CNE2 radiation sensitivity of nasopharyngeal carcinoma cells. RNA interference technology was used to silence the atg5 gene and autophagy inhibition cell model was constructed. Rapamycin and chloroquine were treated respectively on cells with X-ray 5Gy irradiation. Cells' growth status were observed for 8 days and control group was set. The cell viability was detected by MTT assay and colony formation assay, and the cell cycle was analyzed by flow cytometry...
November 30, 2016: Cellular and Molecular Biology
R Gul Guven, N Aslan, K Guven, F Matpan Bekler, O Acer
In this study, polyphenol oxidase (PPO) from corn tassel  was extracted and partially purified through  (NH4)2SO4 precipitation and gel filtration chromatography. Optimal temperatures for subsrates catechol and 4-methyl catechol were 40 °C and 30 °C, respectively. The optimal pH values were 8.0 for catechol and 6.0 for 4-methyl catechol. Catechol was the most suitible substrate (Km: 3.48 mM, Vmax: 1.0 Abs./ min.). The moleculer mass of PPO was determined as 158 kDa. In this work, sodium azide, ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS) were found to inhibit the enzyme activity as 26...
November 30, 2016: Cellular and Molecular Biology
S Köprücü, M Yaman
In this study was to examined the morphology, histology and histochemically the digestive tract of European catfish, Silurus glanis. Two-four age S. glanis obtained from Çelik Lake (Gölbaşı-Adıyaman, Turkey). Six S. glanis, 59-71 cm length, 1.5-3.2 kg weight used for in the investigation. The body cavity was opened and samples of digestive tract (esophagus, stomach and intestine) were fixed in neutral buffered 10% formalin and embedded in paraffin. Dewaxed section (5-6 µm) were deparaffinized and stained with haematoxylin and eosin (H&E), Crossman triple, periodic acid-shiff (PAS), alcian blue (AB, pH 1...
November 30, 2016: Cellular and Molecular Biology
Y H Lee, S-C Bae
The aim of this study was to determine whether intercellular adhesion molecule-1 (ICAM-1) polymorphisms are associated with susceptibility to vasculitis or rheumatoid arthritis (RA). Meta-analyses were performed to assess the associations between K469E and G241R polymorphisms of ICAM-1 and vasculitis or RA. A total of 12 studies on 1,368 patients and 1,922 controls, which comprised 8 vasculitis studies and 4 RA studies, were included in the meta-analysis. We found no significant association between vasculitis and K469E E allele among the various subjects (OR = 1...
October 31, 2016: Cellular and Molecular Biology
P Wang, P Dong, X Yang
Some studies investigated the association of antisense non-coding RNA in the INK4 locus (ANRIL) rs2383207 polymorphism with coronary artery disease (CAD) risk. However, the result was still inconsistent. The aim of this study was to investigate whether there is an association between the ANRIL rs2383207 polymorphism and CAD risk. We carried out a PubMed (Medline), EMBASE database search covering all published articles. The strength of association between ANRIL rs2383207 polymorphism and CAD risk was assessed by calculating OR with 95% CI...
October 31, 2016: Cellular and Molecular Biology
X Lin, M Z Qureshi, R Attar, S Khalid, F Tahir, A Yaqub, A Aslam, I Yaylim, L K De Carlos Back, A A Farooqi, M Ismail
In 1960 researchers reported that balanced translocation between chromosomes 22 and 9 resulted in the generation of Philadelphia chromosome. This breakthrough revolutionized our knowledge related to leukemia biology and contemporary studies revealed that chromosomal translocation resulted in the fusion between the 5' segment of BCR gene and 3' segment of the ABL gene to form BCR/ABL fusion gene. Research over the years has progressively and systematically improved our understanding of the genetic and proteomic basis of Leukemia...
October 31, 2016: Cellular and Molecular Biology
N Iranshahi, P Zafari, K H Yari, E Alizadeh
Breast cancer, with a lifelong risk of one in nine, is the most common cancer among women. In Iran, breast cancer is one of the growing and important women's health problems. Several environmental, genetic and epigenetics factors have been suggested to have a role in breast cancer development. Epigenetics alterations are heritable changes in gene expression that occur without causing any change in DNA sequence. DNA methylation as a main epigenetics modification in human cancer is found as a promising biomarker in early detection of breast cancer...
October 31, 2016: Cellular and Molecular Biology
M Gholami, S H Hafezian, G Rahimi, A Farhadi, Z Rahimi, D Kahrizi, S Kiani, H Karim, S Vaziri, S Muhammadi, F Veisi, K Ghadiri, H Shetabi, J Zargooshi
There are two allelic forms of A1 and A2 of β-casein gene in dairy cattle. Proteolytic digestion of bovine β-casein A1 type produces bioactive peptide of β-casomorphin-7 known as milk devil. β-casomorphin-7 causes many diseases, including type 1 diabetes, cardiovascular disease syndrome, sudden death and madness. The aim of the present study was to determine the different allelic forms of β-casein gene in Iranian Holstein, Simmental and native cattle in order to identify A1 and A2 variants. The blood samples were collected randomly and DNA was extracted using modified salting out method...
October 31, 2016: Cellular and Molecular Biology
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