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Journal of AOAC International

Ahmed Mostafa, Heba Shaaban, Mishal Almousa, Mishal Al Sheqawi, Muntdher Almousa
Background : Considering the environmental impact of analytical procedures necessitates replacing the polluting analytical methods with green alternatives. Objective : This study aims to develop and validate a multivariate curve resolution-alternating least-squares (MCR-ALS) method with correlation constraint for the simultaneous determination of theophylline, ambroxol, and guaifenesin as target analytes in the presence of methylparaben and propylparaben as interfering components. In addition, a partial least-squares regression (PLSR) method was also developed and optimized...
September 21, 2018: Journal of AOAC International
Marwa A A Ragab, Mohamed H Abdel-Hay, Hytham M Ahmed, Sara M Mohyeldin
Background: A validated method based on capillary zone electrophoresis coupled with a diode array detector (CZE-DAD) was investigated for analyzing binary mixture of ibuprofen (IBU) and phenylephrine (PHE) in their bulk and combined dosage form. Objective: This binary mixture is a challenging one as IBU is acidic and PHE is alkaline, which may affect their simultaneous analysis using CZE. The literature lacks any CZE report for IBU and PHE simultaneous analysis. Methods: Fused silica capillary (85 cm × 75 μm id) was used, and the electrolyte was a 50 mM borate buffer adjusted to pH 11 with 0...
September 19, 2018: Journal of AOAC International
Mamdouh Allawzi, Hussein Allaboun, Atheer Almasri
Background: Experimental investigation of supercritical fluid extraction (SFE) of active ingredients from rosemary herb has been performed. Carbon dioxide (CO₂) was used as a solvent with ethanol as a trapping agent. This work showed that the SFE can be an exceptional alternative to the use of chemical solvents. Objective: The effect of temperature and pressure on the extraction process was investigated to increase the yield of the extracted essential components. Methods: The types of extracted compounds from rosemary were specified and analyzed using GC-MS...
September 19, 2018: Journal of AOAC International
Naoto Hieda, Yoshihiro Ikeuchi, Ichirou Matsuno
Background: Reliable measurements of iodine are essential for ensuring the quality of infant formula. The AOAC Official Method 2012.15 for iodine tends to produce higher results in the presence of carbon remaining in the final test solution after digestion with alkaline dissolution. This is partly because of the lack of countermeasures for signal enhancement induced by coexisting carbon in Method 2012.15. Objective: To obtain more reliable values for infant formulas, we undertook an experiment. Methods: We modified the protocol by adding carbon in the form of methanol to both the standard solutions and the final test solutions...
September 18, 2018: Journal of AOAC International
Ying Liu, Michael Chan, Ebersole Blake, Hong Sy, Paula N Brown
Background: Kombucha is a nonalcoholic, fermented tea beverage that has recently received negative attention because of documented concentrations of ethanol in excess of allowable limits of ≥0.05% alcohol by volume (ABV). Objective: Our previously reported headspace GC with flame-ionization detection (GC-FID) method was adopted by the AOAC Expert Review Panel as First Action Official Method SM 2016.12 in September 2016 based on published single-laboratory validation study results. This paper describes the corresponding multilaboratory study using this method to further validate its performance parameters...
September 18, 2018: Journal of AOAC International
Yuangui Yang, Yanli Zhao, Zhitian Zuo, Yuanzhong Wang
Background: Paris polyphylla var. yunnanensis (PPY) is used in the clinical treatment of tumors, hemorrhages, and anthelmintic. Objective: The aim of this study is to determine total flavonoids of PPY in the Yunnan and Guizhou Provinces, China. Methods: In this study, total flavonoids were determined by UV spectrophotometry at first. Then, Fourier transform mid-infrared (FT-IR) based on various pretreatments include standard normal variate (SNV), first derivative (FD), second derivative (SD), Savitzky-Golay (SG), and orthogonal signal correction (OSC) were investigated...
September 18, 2018: Journal of AOAC International
Cortlandt P Thienes, Jongkit Masiri, Lora A Benoit, Brianda Barrios-Lopez, Santosh A Samuel, Richard A Krebs, David P Cox, Anatoly P Dobritsa, Cesar Nadala, Mansour Samadpour
Background: Concerns about the contamination of meat products with undeclared meats, and new regulations for the declaration of meat adulterants have established the need for a rapid test to detect beef adulteration to 0.1% sensitivity. Objective: To address this need, Microbiologique, Inc. has developed an ELISA that can quantify the presence of beef down to 0.1% (w/w) in cooked pork, horse, chicken, goat, and sheep meat. Results: The beef-authentication ELISA has an analytical sensitivity of 0.00022 and 0...
September 18, 2018: Journal of AOAC International
Bertil Nshime, James Koedam, Brian Stanton, Quynh Tran, Prescott Chen
Background: The determination of cyanocobalamin (vitamin B12) and biotin has always been challenging because of the lack of a chromophore for biotin and trace level input for vitamin B12 in supplements. Microbiological assay methods are currently used for quantitation. However, these methods are time consuming, may lack specificity, and have high imprecision. Objective: Our laboratory developed and validated an LC method for the simultaneous quantitation of vitamin B12 and biotin. Methods: This LC method uses a single quadruple mass analyzer to detect biotin and vitamin B12 at m/z 245...
September 18, 2018: Journal of AOAC International
Zhibang Chen, Lina Huang, Lezhou Yi, Xiaoxia Mai, Hanke Li, Qiong Li, Tongmei Ma
Background: Bath-type ultrasonic-assisted extraction (UAE) has been developed as one of the most important sample pretreatment methods, especially for batch-sample pretreatment. So far, however, requirements for the performance of bath-type UAE equipment have not been standardized, nor has a suitable evaluation method that can be used to judge the feasibility of ultrasonic equipment for extraction been presented in the available regulations or standards. Objective: A simple and efficient method that can be used to evaluate the performance of bath-type UAE equipment is necessary to be proposed and established...
September 11, 2018: Journal of AOAC International
Dawn Qian Liu, Mitsuharu Sato, Wei Ling Tan, Grace Pei Wen Khoo, Kiel Fisher, Hannah Meibers, Damian Guang Wei Foo, Helen Choon Sen Phang, Patrick Bird, M Joseph Benzinger, Erin Crowley, James Agin, David Goins, Paul King Tiong Chiew, Rosemary Sok Pin Tan
VereBeef™ Detection Kit, incorporating both multiplex PCR and microarray technologies on a lab-on-chip platform, is intended for qualitative detection and differentiation of Escherichia coli O157:H7, E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121, E. coli O145, Shiga toxin-producing E. coli (STEC) virulence factors ( stx1A, stx2A, eae ), and Salmonella species in one test using raw beef trim samples. This product underwent extensive evaluations, including inclusivity-exclusivity, method comparison, robustness, lot-to-lot variability, and stability studies...
September 11, 2018: Journal of AOAC International
Susan Alles, Brooke Roman, Quynh-Nhi Le, Edan Hosking, Wesley Colangelo, Eric Tovar, Preetha Biswas, Mark Mozola, Robert Donofrio
Background: The ANSR method is based on isothermal nucleic acid amplification technology. The modifications to assay components improve sensitivity of the assay and robustness of the internal positive control. Objective: A Performance Tested Method SM validation study was conducted to assess performance of a modified version of the ANSR® for Escherichia coli O157:H7 method. Methods: The validation study included inclusivity/exclusivity, matrix, robustness, accelerated stability, and independent laboratory testing...
September 11, 2018: Journal of AOAC International
Eri Matsumoto-Tanibuchi, Toshiaki Sugimoto, Toshiyuki Kawaguchi, Naoki Sakakibara, Michiaki Yamashita
Background: Seaweed and seafood often contain both inorganic and organic arsenic compounds showing distinct toxicities. Speciation must be taken into account when determining the concentrations of arsenic compounds and how they relate to overall toxicity. Objective: An analytical method for the quantitation of inorganic arsenic was validated in seaweed and seafood. Methods: Food samples were heated at 100°C in 0.3 mol/L nitric acid. Arsenic speciation was quantitatively determined by LC-inductively coupled plasma-MS (LC-ICP-MS) using an octadecilsilane (ODS) column with a mobile phase containing an ion-pair reagent...
September 11, 2018: Journal of AOAC International
Sandra Salleres Alonso, Irune González, Naiara Linaza, Erlantz Ramos, Mónica Fernández, Roberto González, Sonia Maza, Arrate Jaureguibeitia
Background: Sulfites are some of the oldest and most widespread preservatives in our food supply. They are food additives that have antioxidant properties, but they are also recorded as allergens by the main international regulatory bodies on food safety because of their adverse health effect. Hence, sulfites maximum concentration in foodstuff is regulated and they must be ensured by the agro-food processing industries. The most widely used technique for the quantification of sulfites is the Modified Monier-Williams (AOAC Official Method <bold>990...
August 29, 2018: Journal of AOAC International
Markus Roggen
In an industry that has only recently and slowly moved out of the shadows of illegality, regulations and guidelines for cannabis product safety are just catching up to the standards of other industries. Often, our industry has had to introduce best practices and self-regulation to ensure the safety of our products and customers, as well as to help any regulating body in building such frameworks. This article will focus on how OutCo has implemented Hazard Analysis and Critical Control Point (HACCP) protocols to ensure the safety of every aspect of the production pipeline...
August 29, 2018: Journal of AOAC International
Linda E Klumpers, David L Thacker
Cannabis has been used as a medicinal plant for thousands of years. As a result of centuries of breeding and selection, there are now over 700 varieties of cannabis that contain hundreds of compounds, including cannabinoids and terpenes. Cannabinoids are fatty compounds that are the main biological active constituents of cannabis. Terpenes are volatile compounds that occur in many plants and have distinct odors. Cannabinoids exert their effect on the body by binding to receptors, specifically cannabinoid receptors types 1 and 2...
August 23, 2018: Journal of AOAC International
Md A Ali, Wang Hui, Guangen Lin, Fang F Dang, Shuang Wu, Chaoxin Man, Yujun Jiang
Background: Bifidobacterium animalis subsp. lactis HN019 is widely used as a probiotic in various dairy preparations. To ensure optimal health benefits, an adequate number of probiotics should be viable in products, but the culture-based methods require longer periods to account for the strain. Objective: Develop a standard curve to account viable load of B. animalis subsp. lactis HN019 by real-time PCR. Methods: The growth curve was developed according to plate count method, and cycle threshold (CT ) curve was prepared using CT values...
August 22, 2018: Journal of AOAC International
Linda Beck, Scott G Coates, Jay Gee, Ted Hadfield, Paul Jackson, Paul Keim, Luther Lindler, Vicki Olson, Eileen Ostlund, Francisco Roberto, James Samuel, Shashi Sharma, Sandra Tallent, David M Wagner
There has been a proliferation in the developmentof biological threat agent detection technologies foruse in the field by first responders and private-sectorend-users as well as in Department of Defense(DoD) applications in which active combat maybe occurring and in other parts of the world. Incontrast to the proliferation of detection methodology,there has been a lack of standards defining therequired performance of these technologies. Standardsare necessary to demonstrate the performanceand limitations of the tools, providing confidencein the data to allow appropriate response actions byend-users and responders...
August 22, 2018: Journal of AOAC International
Harish K Janagama, Tam Mai, Sukkhyun Han, Lourdes Nadala, Cesar Nadala, Mansour Samadpour
Background: The presence of microbial contaminants such as Brettanomyces in wine can lead to undesirable wine. Therefore, monitoring for the presence of these spoilage organisms is critical for winemakers to ensure the quality of their end product. Objective: To address this problem, Molecular Epidemiology, Inc. (MEI, Seattle, WA) has developed a wine-spoilage organism detection kit consisting of a multiplex PCR DNA dipstick that simultaneously detects these organisms. Methods: Wine samples obtained from local wineries that tested negative by routine microbiological culture were spiked with the target microorganisms, while samples that were designated as spoiled by the wineries were used as-is without spiking for assessing the performance characteristics of the DNA dipstick assay...
August 20, 2018: Journal of AOAC International
Barry V McCleary
A method for measurement of total dietary fiber (TDF) has been validated. This method is applicable to plant materials, foods, and food ingredients as consumed, consistent with the 2009 CODEX definition (ALINORM 09/32/REP), and measures insoluble dietary fiber (IDF) and soluble dietary fiber (SDF), comprising SDF that precipitates in the presence of 78% ethanol (SDFP) and SDF that remains soluble in the presence of 78% ethanol (SDFS). The method is an update of AOAC Method 2009.01 and addresses each of the issues identified by analysts in using that method over the past 8 years...
August 14, 2018: Journal of AOAC International
Javad Tashkhourian, Hamed Valizadeh, Abdolkarim Abbaspour
Background: Metal-organic frameworks (MOFs) are classified as microporous materials. They have large surface areas, low framework densities, accessible cages, and tunnels with modifiable pores. Objective: The MOF MIL-101(Cr) with a large pore volume was synthesized hydrothermally and used in the electrochemical reactions as an electrocatalyst for the determination of ascorbic acid (AA). The synthesized MOF was characterized by scanning electron microscopy and X-ray powder diffraction. Methods: The electrocatalytic behavior of a carbon-paste electrode modified with MIL-101(Cr) was studied through electro-oxidation of AA by cyclic voltammetry and square wave voltammetry...
August 13, 2018: Journal of AOAC International
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