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Journal of AOAC International

Andrew Denhartigh, Lindsay Reynolds, Katherine Palmer, Frank Klein, Jennifer Rice, John J Rejman
A validation study was conducted for an immunochromatographic method (BetaStar® Advanced for Beta-lactams) for the detection of beta-lactam residues in raw, commingled bovine milk. The assay detected amoxicillin, ampicillin, cloxacillin, penicillin, cephapirin, and ceftiofur below the U.S. Food and Drug Administration tolerance levels but above the maximum sensitivity thresholds established by the National Conference on Interstate Milk Shipments. The results of internal and independent laboratory dose-response studies employing spiked samples were in agreement...
May 18, 2018: Journal of AOAC International
Camila Tavares Rebouças, Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Background: A green analytical chemistry method was developed for quantification of enrofloxacin in tablets. The drug, a second-generation fluoroquinolone, was first introduced in veterinary medicine for the treatment of various bacterial species. Objective: This study proposed to develop, validate, and apply a reliable, low-cost, fast, and simple IR spectroscopy method for quantitative routine determination of enrofloxacin in tablets. Methods: The method was completely validated according to the International Conference on Harmonisation guidelines, showing accuracy, precision, selectivity, robustness, and linearity...
May 18, 2018: Journal of AOAC International
Eglal R Souaya, Mona A Khorshed, Mahmoud M Ghuniem
Background: There is increasing concern about the health effects on humans due to continuous consumption of fruit juices contaminated with metals. Objective: Quadrupole inductively coupled mass spectrometry (Q-ICP-MS) was optimized, validated, and applied in routine analysis for determination of As, Pb, Cd, Sb, Hg, Cu, Zn, Fe, Al, Cr, Sn, Co, Mn, and Ni in fruit juice beverages. Methods: Several validation parameters, such as detection limits (DLs), quantification limits, linearity, accuracy, and measurement uncertainty (MU), were studied under optimized Q-ICP-MS conditions...
May 18, 2018: Journal of AOAC International
Florina-Maria Copaciu, Dorina Simedru, Maria-Virginia Coman
In the present work, a procedure to determine three textile azo dyes, chromium-complexes [Nylosan Dark Brown (NDB), Lanasyn Dark Brown (LDB), and Lanasyn Red (LR)], from wastewater using solid-phase extraction (SPE) followed by LC-electrospray ionization negative mode tandem mass spectrometry (LC-ESI(-)-MS/MS) has been developed. The extraction/concentration and recovery degree of these dyes from liquid matrices were done on Strata WAX/NH₂ cartridges. The chromatographic separation was performed using a Luna C18 (2) 100Å column by isocratic elution with a methanol-acetonitrile-water (0...
May 18, 2018: Journal of AOAC International
Wendy C Andersen, Christine R Casey, Tara J Nickel, Susan L Young, Sherri B Turnipseed
Background : Triphenylmethane dyes and metabolites are known or suspected mutagens and areprohibited in animals intended for human consumption. Despite toxicity, triphenylmethane dyes are used illegally as inexpensive treatments for fungal and parasite infections in aquatic animals. Objective: AOAC INTERNTIONAL Official Method ℠ 2012.25 for the LC-MS/MS determination of malachite green, crystal violet, brilliant green, and metabolites leucomalachite green and leucocrystal violet in seafood products was previously validated for finfish (trout, salmon, catfish, and tilapia) and shrimp, but had not been fully validated for other types of aquacultured products such as eel, molluscan shellfish, or frog or for processed seafoods...
May 18, 2018: Journal of AOAC International
Shan Zhang, Shuai Zhao, Sai Wang, Jiahui Liu, Yiyang Dong
Lateral flow immunochromatographic strips based on colorants of aptamer-functionalized nanogold particles weredeveloped for the detection of micropollutants aflatoxin B1 (AFB1) and chloramphenicol (CAP). The lateral flow immunochromatographic strip was based on a competitive reaction of thiolated-aptamer between micropollutants and bio-DNA probe-streptavidin as capture material immobilized at the test line. General crucial parameters that might influence the sensitivity have been systematically investigated...
May 9, 2018: Journal of AOAC International
David Ankrapp, Benjamin Schaus, Lauren Clements, Frank Klein, Jennifer Rice, John Rejman
A validation study was conducted for an immunochromatographic method (BetaStar® Advanced for Tetracyclines) for detection of tetracycline antibiotic residues in raw, commingled bovine milk. The assay was demonstrated to detect tetracycline, chlortetracycline, and oxytetracycline at levels below the FDA tolerance levels but above the maximum sensitivity thresholds established by the National Conference on Interstate Milk Shipments. Results of internal and independent laboratory dose-response studies employing spiked samples were in agreement...
May 9, 2018: Journal of AOAC International
Mihail Simion Beldean-Galea, Florina-Maria Copaciu, Maria-Virginia Coman
The textile industry uses many raw materials (natural and synthetic dyes and fibers) and different dyeing techniques that can be considered important pollutants with a negative impact on the environment (toxic working conditions, discharged wastewater, and contamination). Although synthetic dyes are intensively used, offer a wide range of colors and hues and properties of adhesion, longevity, and resistance to sunshine and chemical processes, and are cost-effective, they have begun to be restricted by many textile producers because they are nonbiodegradable and have toxic, carcinogenic, and mutagenic effects that generate some imbalances in plant, animal, and human life...
May 9, 2018: Journal of AOAC International
Ľubica Piknová, Veronika Janská, Tomáš Kuchta, Peter Siekel
Background : Hazelnuts, being a frequent agent of allergenic reactions, need to be detected in food products. Thus, it is necessary to develop and further investigate appropriate methods for detection. Objective : The aim of the study was to compare the analysis of nut pastes (peanut paste spiked with different amounts of hazelnut paste) as a model of contamination of confectionery. Methods : Real-time PCR and sandwich ELISA (RidaScreen Hazelnut Fast Kit) were used. Results : For real-time PCR, LOQ of 2 mg/kg and a quantification range from 2 to 10 000 mg/kg were determined...
May 9, 2018: Journal of AOAC International
Jie Li, Ji Zhang, Zhitian Zuo, Hengyu Huang, Yuanzhong Wang
Background : Swertia nervosa (Wall. ex G. Don) C. B. Clarke, a promising traditional herbal medicine for the treatment of liver disorders, is endangered due to its extensive collection and unsustainable harvesting practices. Objective : The aim of this study is to discuss the diversity of metabolites (loganic acid, sweroside, swertiamarin, and gentiopicroside) at different growth stages and organs of Swertia nervosa using the ultra-high-performance LC (UPLC)/UV coupled with chemometric method. Methods : UPLC data, UV data, and data fusion were treated separately to find more useful information by partial least-squares discriminant analysis (PLS-DA)...
May 9, 2018: Journal of AOAC International
Anli Gao, Jennifer Fishcher-Jenssen, Colin Cooper, Honghong Li, Jiping Li, Shu Chen, Perry Martos
Background : Ready-to-eat (RTE) meats, fruits, and vegetables contaminated by Shiga toxinproducing Escherichia coli (STEC) raise serious concerns because they are often consumed directly without further processing. Objective: To evaluate a multiplex PCR for the detection of STEC across food categories. Methods: Samples (25 g) from seven RTE meat and nine fruit and vegetable matrices were inoculated with each of seven STEC (O157:H7, O26, O121, O145, O45, O103, O111) strains targeting 10 CFU/25 g, enriched in 225 mL of modified tryptone soya broth (mTSB), and tested by a multiplex real-time PCR for stx and eae genes, following U...
May 3, 2018: Journal of AOAC International
Medhat A El-Naggar, Aisha M Alrajhi, Moustafa M Fouda, Eman M Abdelkareem, Tamer M Thabit, Nahla A Bouqellah
Background: Deoxynivalenol (DON) is one of the most important fungal mycotoxins excretedby different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems. Objective: This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains. Methods: Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA...
May 3, 2018: Journal of AOAC International
Kamila Rybczyńska-Tkaczyk, Teresa Korniłłowicz-Kowalska
The aim of this study was the evaluation of activities of versatile peroxidase (VP) in cultures of Clonostachys sp. ( Gliocladium sp.) strains during biotransformation of 0.2% alkali lignin (AL). The principal component analysis (PCA) method was applied to determine the main factors and correlation between biotransformation of 0.2% AL, activity of VPs, pH value, and Clonostachys sp. strains. The biotransformation of 0.2% AL in cultures of microscopic fungi included decolorization (medium lightening) and colorization (darkening of the medium)...
May 3, 2018: Journal of AOAC International
Dominika B Matuszek, Krystian Wojtkiewicz
Background: This paper describes the use of fluorescence induced by UV radiation to evaluate the share of tracer in feed mixture. Methods: For the purpose of this study, three substances were used. They are as follows: Tinopal, Rhodamine B, and Uranine. Tracer in the form of maize or kardi was added to chicken feed before the mixing process. Grains used in the process were grinded in the mill sieve with a mesh size of 4 and 6 mm. The drawn samples of the mixture were illuminated with UV radiation to make grain tracer light, and then the photo was taken with a digital camera...
May 3, 2018: Journal of AOAC International
Jorge C Pereira, Jorge M C Marques, Elżbieta Włodarczyk, Bożena Fenert, Paweł K Zarzycki
Planar chromatography and related techniques [micro-planar chromatography, micro-TLC, or paper-based microfluidic devices (μPADs)] present several advantages in analytical applications, such as simplicity, low cost of analysis, and the ability to work with raw complex samples without the involvement of time-consuming prepurification steps. By using commonly applied planar chromatographic systems and μPADs devices, stationary phases (silica and cellulose based), different solvent mixtures (methanol-water and dichloromethane-methanol), and proportions varying from 0 to 100% (v/v), micro-TLC migration profiles of several dyes described in terms of characteristic of chromatographic parameters (retardation factor, peak base width, and asymmetry factor) were investigated...
May 3, 2018: Journal of AOAC International
Tam L Mai, Shao-Lei Sung, Anna Shapovalova, Harish K Janagama, Long Vuong, Alex Friedrich, Dylan Johnson, Lourdes M Nadala, Van Nguyen, Elpidio Cesar Nadala, Mansour Samadpour
The Microfilm™ Test System is intended for quantitative microbiology and consists of three types of Microfilmsfor aerobic plate count (Microfilm APC), total coliform and Escherichia coli count (Microfilm TCEc), and yeast and mold count (Microfilm YMC). This study evaluated the performance of the Microfilm Test System against International Organization for Standardization (ISO) methods on 20 food matrixes and 2 environmental surfaces. Ruggedness, robustness, and stability were also determined, while inclusivity and exclusivity studies were performed on Microfilm TCEc and YMC...
May 3, 2018: Journal of AOAC International
Renata Świderska-Dąbrowska, Krzysztof Piaskowski, Paweł K Zarzycki
There is great interest in the search for multifunctional waste-based materials that may be applied as environmentally friendly adsorbents. Iron-rich sludge from ground drinking-water treatment plants may be considered a potential adsorbent for various water contaminants. This material is generated during ground water purification because of the excess of metal ions in water (Fe, Mn). In practice, this sludge is frequently disposed of as waste material and, so far, is not commonly applied as the adsorption base...
May 3, 2018: Journal of AOAC International
Shuai Zhao, Shan Zhang, Sai Wang, Jiahui Liu, Yiyang Dong
A methodology of lateral flow immunochromatographic strip based on aptamer was developed for on-site detectionof the small molecule micropollutants. In the present study, we try for the first time to investigate the feasibility of developing a strip assay for the analysis of micropollutants as methodological prototypes by combining the high selectivity and affinity of aptamers with the unique optical properties of nanogolds. This quantitative method was based on the competition for the aptamer between targets and DNA probes...
May 3, 2018: Journal of AOAC International
Haisheng Zhang, Jing Xue, Huanxia Zhao, Xinshuai Zhao, Huanhuan Xue, Yuhan Sun, Wanrui Xue
Background : The composition and sequence of amino acids have a prominent influence on theantioxidant activities of peptides. Objective : A series of isolation and purification experiments was conducted to explore the amino acid sequence of antioxidant peptides, which led to its antioxidation causes. Methods : The degreased apricot seed kernels were hydrolyzed by compound proteases of alkaline protease and flavor protease (3:2, u/u) to prepare apricot seed kernel hydrolysates (ASKH). ASKH were separated into ASKH-A and ASKH-B by dialysis bag...
May 3, 2018: Journal of AOAC International
Jian-Xun Li, Xiao-Ying Li, Qiao-Ying Chang, Yan Li, Ling-He Jin, Guo-Fang Pang, Chun-Lin Fan
Because of its unique characteristics of accurate mass full-spectrum acquisition, high resolution, and fast acquisition rates, GC-quadrupole-time-of-flight MS (GC-Q-TOF/MS) has become a powerful tool for pesticide residue analysis. In this study, a TOF accurate mass database and Q-TOF spectrum library of 439 pesticides were established, and the parameters of the TOF database were optimized. Through solid-phase extraction (SPE), whereby pesticides are extracted from fruit and vegetable substrates by using 40 mL 1% acetic acid in acetonitrile (v/v), purified by the Carbon/NH₂ SPE cartridge, and finally detected by GC-Q-TOF/MS, the rapid analysis of 439 pesticides in fruits and vegetables can be achieved...
May 3, 2018: Journal of AOAC International
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