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Journal of AOAC International

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https://www.readbyqxmd.com/read/28644779/cellular-light-scattering-for-the-identification-of-bacteria-and-its-application-to-the-identification-of-staphylococcus
#1
David L Haavig, Kaylagh R Hollen, Abbey E Debruin, Shelbi R Lisecki, Nicole C Shoup, Mickey J H Stimac, Anthony M Treloar, Zachary N Jodoin, Margaret S Bohm, Josh S Sharp
Rapid identification of bacteria is critical in clinical and food safety applications. This paper describes anovel instrument and data analysis method for identifying bacteria based on the measurement of laser light scattering as the beam interacts with bacterial cells suspended in water. A description of the technology is followed by an identification performance study for a set of strains from the genus Staphylococcus (the inclusive target organisms) and a set of non-Staphylococcus strains (the exclusive organisms)...
June 23, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28631591/comparative-evaluation-of-veriflow-%C3%A2-listeria-species-to-usda-culture-based-method-for-the-detection-of-listeria-spp-in-food-and-environmental-samples
#2
Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Amrita Puri, Benjamin J Pascal, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Listeria species (Veriflow LS) isa molecular-based assay for the presumptive detection of Listeria spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile) and ready-to-eat (RTE) food matrixes (hot dogs and deli meat). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only a 24 h enrichment for maximum sensitivity. The Veriflow LS system eliminates the need for sample purification, gel electrophoresis, or fluorophore-based detection of target amplification and does not require complex data analysis...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28631590/comparative-evaluation-of-veriflow-%C3%A2-salmonella-species-to-usda-and-fda-culture-based-methods-for-the-detection-of-salmonella-spp-in-food-and-environmental-samples
#3
Amrita Puri, Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Salmonella species (Veriflow SS)is a molecular-based assay for the presumptive detection of Salmonella spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile), dairy (2% milk), raw meat (20% fat ground beef), chicken carcasses, and ready-to-eat (RTE) food (hot dogs). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only an 18 h enrichment for maximum sensitivity...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623662/advances-in-the-analysis-of-water-and-wastewater-samples-using-various-sensing-protocols-and-microfluidic-devices-based-on-pad-and-%C3%AE-tas-systems
#4
Krzysztof Piaskowski, Renata Świderska-Dąbrowska, Aleksandra Kaleniecka, Paweł K Zarzycki
The main goal of this review is to summarize practical approaches concerning the application of microfluidic systems for the analysis of various biomarkers and pollutants, as well as microbes, in water and wastewater matrixes. This problem involves multidisciplinary expertise combining research knowledge from various areas, including wet chemistry, biochemistry, physical chemistry, molecular biology, genetics, signal processing, microelectronics material science, and separation science. It has been documented that fairly primitive but fast and inexpensive screening methods involving paper-based analytical devices (PADs) and micro total analytical systems (μTAS) can be considered as serious alternatives to their more advanced counterparts such as GC, HPLC, and capillary electrophoresis coupled to various sophisticated detectors (e...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623661/graphene-nanolayers-as-a-new-method-for-bacterial-biofilm-prevention-preliminary-results
#5
Łucja Dybowska-Sarapuk, Andrzej Kotela, Jakub Krzemiński, Marta Wróblewska, Halina Marchel, Magdalena Romaniec, Paweł Łęgosz, Małgorzata Jakubowska
Biofilms are microbial communities of surface-attached cells embedded in a self-produced extracellular matrix. They have been found to play a role in a wide variety of infections, including catheter-related urinary tract and bloodstream infections, and, therefore remain a significant source of morbidity and mortality among the world's population. Recently, much attention has been devoted to the prevention of biofilm formation on implant surfaces. Nanomaterials such as graphene, characterized by antibacterial activity and low toxicity to human cells, are promising candidates for biomedical applications...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623660/the-method-of-coating-fe%C3%A2-o%C3%A2-with-carbon-nanoparticles-to-modify-biological-properties-of-oxide-measured-in-vitro
#6
Tomasz Niemiec, Mariusz Dudek, Natalia Dziekan, Sławomir Jaworski, Aleksandra Przewozik, Emilia Soszka, Anna Koperkiewicz, Piotr Koczoń
The coating of nanoparticles on materials for medical application [e.g., the coating of Fe₃O₄ nanopowder (IONP) with a carbon nanolayer] serves to protect and modify the selected biological, physical, and chemical properties of the coated material. Increases in chemical stability, changes in biocompatibility, and a modified surface structure are examples of the effects caused by the formation of carbon coatings. In the current study, Fe₃O₄ nanoparticles were coated with a carbon nanolayer (IONP@C) in a plasmochemical reactor (using radio-frequency plasma-enhanced chemical vapor deposition methods) under various experimental conditions...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623657/aptamer-magnetic-bead-quantum-dot-sandwich-assays-for-foodborne-pathogen-detection-pros-cons-and-lessons-learned
#7
John G Bruno, Jeffrey C Sivils, Taylor Phillips
DNA and RNA aptamers have been extensively investigated as potential competitors for antibodies for a variety of applications including food safety testing. Ultrasensitive fluorescence detection of foodborne pathogenic bacteria as low as 1-10 cells/mL has been achieved using aptamers coupled to quantum dots in clear pristine buffers for environmental sample detection. Quantum dots offer other advantages, including single UV or blue light source multiplex (multicolored) detection. However, quantum dots can exhibit decreased fluorescence in some food matrixes and even completely fail to fluoresce in some fatty matrixes, as documented in this report...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623656/quantum-dots-as-components-of-electrochemical-sensing-platforms-for-the-detection-of-environmental-and-food-pollutants-a-review
#8
María Pedrero, Susana Campuzano, José M Pingarrón
The determination of organic and inorganic environmental and food pollutants is a key matter of concern in analytical chemistry due to their effects as a serious threat to human health. Focusing on this issue, several methodologies involving the use of nanostructured electrochemical platforms have been recently reported in the literature. Among these methods, those employing the use of quantum dots (QDs) stand out because of features such as signal amplification, good reproducibility and selectivity, and the possibility for multiplexed detection, and because they preserve the outstanding characteristics of electrochemical methodologies with respect to simplicity, ease-of-use, and cost-effective instrumentation...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623655/the-thin-layer-microchromatography-%C3%AE-tlc-and-tlc-fid-technique-as-a-new-methodology-in-the-study-of-lubricating-oils
#9
Paulina Nowak, Judyta Kosińska, Marta Glinka, Marian Kamiński
This paper concerns the possibility of using TLC coupled with a flame ionization detector (FID) and micro-TLC (μTLC) asprecursors for microfluidized devices of analytical techniques to identify and determine the presence and content of the petroleum/vegetable oil base in the lubricating oils applied in cutting devices (chainsaws). This research is related to the problem of ensuring, in compliance with the requirements of environmental protection, a sufficient level of biodegradability of lubricating oils emitted to the environment during operation of equipment lubricated with these oils...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623654/value-assignment-of-vitamin-d-metabolites-in-vitamin-d-standardization-program-serum-samples
#10
Karen W Phinney, Johanna E Camara, Susan S-C Tai, Lane C Sander, Stephen A Wise, Linde A C De Grande, Linda M Thienpont, Antonio M Possolo, Blaza Toman, Christopher T Sempos, Joseph M Betz, Paul M Coates
Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25-hydroxyvitamin D [25(OH)D], the value assignment of total 25(OH)D in 50 single-donor serum samples was performed using two isotope-dilution LC with tandem MS methods. Both methods are recognized as reference measurement procedures (RMPs) by the Joint Committee for Traceability in Laboratory Medicine. These samples and their assigned values serve as the foundation for several aspects of the VDSP...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623653/miniaturized-temperature-controlled-planar-chromatography-micro-tlc-as-a-versatile-technique-for-fast-screening-of-micropollutants-and-biomarkers-derived-from-surface-water-ecosystems-and-during-technological-processes-of-wastewater-treatment
#11
Magdalena M Ślączka-Wilk, Elżbieta Włodarczyk, Aleksandra Kaleniecka, Paweł K Zarzycki
There is increasing interest in the development of simple analytical systems enabling the fast screening of target components in complex samples. A number of newly invented protocols are based on quasi separation techniques involving microfluidic paper-based analytical devices and/or micro total analysis systems. Under such conditions, the quantification of target components can be performed mainly due to selective detection. The main goal of this paper is to demonstrate that miniaturized planar chromatography has the capability to work as an efficient separation and quantification tool for the analysis of multiple targets within complex environmental samples isolated and concentrated using an optimized SPE method...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28623652/micro-thin-layer-chromatography-fingerprints-of-selected-basil-species-and-their-chemometric-analysis
#12
Mirosław A Hawrył, Ryszard Świeboda, Anna Hawrył, Małgorzata Niemiec, Monika Waksmundzka-Hajnos
The dried aerial parts of 12 plants of Ocimum spp. were extracted with the Soxhlet apparatus usingdichloromethane and methanol as solvents. A micro-TLC system with silica and a normal-phase solvent system (propan-2-ol-n-heptane-formic acid) was used for the chemometric analysis of 12 selected basil methanolic extracts. Some indices of similarity (Pearson's correlation coefficient, determination coefficient, congruence coefficient, and Euclidean, Manhattan (city-block), Chebyshev, and Hausdorff distances) were calculated on the basis of thin-layer chromatograms using ImageJ software...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28621256/detection-and-analysis-of-microbes-bioanalytes-and-micropollutants-focusing-on-food-and-environmental-samples-using-nanoparticle-based-detection-systems-microfluidic-analytical-devices-and-related-techniques
#13
https://www.readbyqxmd.com/read/28573965/characterization-of-croatian-rape-brassica-sp-honey-by-pollen-spectrum-physicochemical-characteristics-and-multielement-analysis-by-icp-oes
#14
Blanka Bilić Rajs, Ivana Flanjak, Jelena Mutić, Vesna Vukojević, Slađana Đurđić, Ljiljana Primorac
Rape (Brassica sp.) unifloral honey from Croatia was characterized by certain physicochemical parameters, micro- and macroelement content, and pollen spectrum, as determined in 21 honey samples. The Brassica sp. pollen type was predominant in the analyzed samples and ranged between 60 and 98%, with Trifolium spp., Robinia pseudoacacia, Rosaceae, Helianthus annuus, Salix spp., and Taraxacum officinale as the main accompanying pollen types. The electrical conductivity mean value was 0.22 ± 0.05 mS/cm and the glucose/fructose ratio mean value was 1...
June 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28560954/development-and-validation-of-a-gc-ms-method-for-the-analysis-of-homogentisic-acid-in-strawberry-tree-arbutus-unedo-l-honey
#15
Irena Brčić Karačonji, Karlo Jurica
To confirm the botanical origin of strawberry tree(Arbutus unedo L.) honey, a liquid-liquid extractionfollowed by GC-MS method was developed for thequantitative determination of homogentisic acid(HGA), the main phenolic compound in this honey.Different parameters affecting extraction, such asthe type and volume of extraction solvents, pH of thesolution, and amount of salt, were optimized. Themethod showed good linearity (r² = 0.9990) over thetested concentration range (50-500 mg/kg) and a lowLOD (0.3 mg/kg)...
May 31, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28550677/mineral-profile-of-children-s-fast-food-menu-samples
#16
Maria Luisa Cervera, Miguel de la Guardia, Manuela Ruiz-de-Cenzano, Arancha Rochina-Marco, Óscar López-Salazar
Children's fast food menus, including hamburgers served with french fries, dessert, and a soft drink, were analyzed to obtain the mineral profile of trace elements. The developed analytical methodology involved sample digestion under pressure inside a microwave oven with a mixture of HNO₃ and H₂O₂ and inductively coupled plasma-optical emission spectrometry. The method was validated by carrying out the analysis of certified reference materials (NIST 1570a spinach leaves, NCS ZC73016 chicken, and NIST 1568a rice flour) and using recovery experiments...
May 26, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28540845/simple-and-rapid-dual-dispersive-liquid-liquid-microextraction-as-an-innovative-extraction-method-for-uranium-in-real-water-samples-prior-to-the-determination-of-uranium-by-a-spectrophotometric-technique
#17
Naeemullah Khan, Mustafa Tuzen, Tasneem Gul Kazi
An innovative, rapid, and simple dual-dispersive liquidߛliquid microextraction (DDLL-ME) approach was used to extract uranium from real samples for the first time. The main objective of this study was to disperse extraction solvent by using an air-agitated syringe system to overcome matrix effects and avoid dispersion of hazardous dispersive organic solvents by using heat. The DDLL-ME method consisted of two dispersive liquidߛrliquid extraction steps with chloroform as the extracting solvent. Uranium formed complexes with 4-(2-thiazolylazo) resorcinol in the aqueous phase and was extracted in extracting solvent (chloroform) after the first dispersive liquidߛrliquid process...
May 25, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28534467/elemental-composition-of-different-slovenian-honeys-using-k0-instrumental-neutron-activation-analysis
#18
Urška Kropf, Vekoslava Stibilj, Radojko Jaćimović, Jasna Bertoncelj, Terezija Golob, Mojca Korošec
The botanical origin of seven types of Slovenian honey was investigated by analysis of their elemental content using k0-instrumental neutron activation analysis. A total of 28 representative samples were collected from beekeepers all over Slovenia in 2 consecutive years. Nineteen of the 37 elements measured were present in amounts above their LOD. The present study suggests that the determination of only alkali elements might be sufficient for the classification of Slovenian honeys according to their botanical origin...
May 23, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28534465/mineral-content-as-a-tool-for-the-assessment-of-honey-authenticity
#19
Milica Jovetić, Jelena Trifković, Dalibor Stanković, Dragan Manojlović, Dušanka Milojković-Opsenica
The present work aims to provide a contribution to the overall investigation of European unifloral honeys with regard to authentication according to botanical and geographical origins. The mineral content of 206 monofloral honey samples of five botanical origins from six different regions in Serbia was investigated by inductively coupled plasma optical emission spectrometry. Chemometric techniques were applied for the classification and differentiation of acacia, sunflower, and linden honey according to botanical origin, as well as acacia honey samples according to regional origin...
May 23, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28534470/development-and-validation-of-a-rapid-and-sensitive-lc-ms-ms-method-for-the-pharmacokinetic-study-of-osimertinib-in-rats
#20
Shan Xiong, Zhipeng Deng, Peilu Sun, Yanling Mu, Mingxing Xue
<p>Osimertinib is a new-generation epidermal growth factor inhibitor for the treatment of non-small cell lung cancer. In the present study, a rapid and sensitive LC with tandem MS method was developed and validated for the determination of osimertinib in rat plasma. Chromatographic separation was carried out on a C18 column using acetonitrile and water containing 0.1% formic acid. The assay was validated over a concentration range of 1.0–1000 ng/mL for osimertinib, with a lower LOQ of 1.0 ng/mL...
May 22, 2017: Journal of AOAC International
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