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Journal of AOAC International

Ardeshir Shokrollahi, Foroogh Ebrahimi
In the present work, a simple, efficient, and environmentally friendly method was proposed for the simultaneous preconcentration and determination of minute quantities of copper and cobalt in water samples and serum by a supramolecular-based ultrasonic-assisted dispersion solidification liquid–liquid microextraction (DSLLME) procedure coupled with flame atomic absorption spectrometry. The procedure is based on the DSLLME of copper and cobalt with decanoic acid–tetrahydrofuran supramolecular solvent (SUPRAS) in the presence of sodium diethyldithiocarbamate, as the complexing agent...
August 15, 2017: Journal of AOAC International
Abiramasundari Arumugam, Amita Joshi, Kamala K Vasu
The present work focused on the application of design of experiment (DoE) principles to the development and optimization of a stability-indicating method (SIM) for the drug imidapril hydrochloride and its degradation products (DPs). The resolution of peaks for the DPs and their drug in a SIM can be influenced by many factors. The factors studied here were pH, gradient time, organic modifier, flow rate, molar concentration of the buffer, and wavelength, with the aid of a Plackett–Burman design. Results from the Plackett–Burman study conspicuously showed influence of two factors, pH and gradient time, on the analyzed response, particularly, the resolution of the closely eluting DPs (DP-5 and DP-6) and the retention time of the last peak...
August 14, 2017: Journal of AOAC International
Hajime Teramura, Gail Betts
MC-Media Pad SA (formerly known as Sanita-kun SA) is a dry rehydratable film medium for the enumeration of Staphylococcus aureus. The performance of the method in a variety of foods was compared with that of ISO 6888-1:1999, Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and Other Species) - Part 1: Technique Using Baird-Parker Agar Medium. The validated matrixes included pastrami, a sliced cooked chicken roll, cooked prawns, cold-smoked salmon, pasta salad, sandwich spread, fresh uncooked pasta, infant cereal, custard, and raw-milk Brie cheese...
August 10, 2017: Journal of AOAC International
Rouhollah Khani, Zeinab Rostami, Ghodsieh Bagherzade, Vahid Khojeh
In this study, for the monitoring and quantification of p-coumaric acid (p-CA) in vinegar, carrot juice, and seed extract from the plant species Silybum marianum (L.) Gaertn, an efficient and low-cost analytical method has been applied. For this purpose, a dispersive liquid-liquid microextraction (DLLME) method, followed by UV-Vis spectrophotometric detection, was used. To form a cloudy solution, a binary mixture containing ethanol as a disperser solvent and chloroform as an extraction solvent was rapidly injected by syringe into a sample solution containing p-CA...
August 10, 2017: Journal of AOAC International
Nathan P Stern, Jatinder Rana, Amitabh Chandra, John Balles
A quantitative ultra-performance LC (UPLC) method was developed and validated to successfully separate, identify, and quantitate the major polyphenolic compounds present in different varieties of sorghum (Sorghum bicolor) feedstock. The method was linear from 3.2 to 320 ppm, with an r² of 0.99999 when using luteolinidin chloride as the external standard. Method accuracy was determined to be 99.5%, and precision of replicate preparations was less than 1% RSD. Characterization by UPLC-MS determined that the predominant polyphenolic components of the sorghum varietals were 3-deoxyanthocyanidins (3-DXAs)...
August 10, 2017: Journal of AOAC International
Karen Phinney, Christopher Sempos, Susan Tai, Johanna Camara, Stephen Wise, John Eckfeldt, Andrew Hoofnagle, Graham Carter, Julia Jones, Gary Myers, Ramon Durazo-Arvizu, W Greg Miller, Lorin Bachmann, Ian Young, Juanita Pettit, Andrew Liu, Stephen Brooks, Kurtis Sarafin, Michael Thamm, Gert Mensink, Markus Busch, Martina Rabenberg, Kevin Cashman, Mairead Kiely, Karen Galvin, Joy Zhang, Michael Kinsella, Kyungwon Oh, Sun-Wha Lee, Chae Jung, Lorna Cox, Gail Goldberg, Kate Guberg, Sarah Meadows, Ann Prentice, Lu Tian, Patsy Brannon, Robyn Lucas, Peter Crump, Etienne Cavalier, Joyce Merkel, Joseph Betz, Grahame Caldwell
The Vitamin D Standardization Program (VDSP) coordinated a study in 2012 to assess the commutability of reference materials and proficiency testing/external quality assurance materials for total 25-hydroxyvitamin D [25(OH)D] in human serum, the primary indicator of vitamin D status. A set of 50 single-donor serum samples as well as 17 reference and proficiency testing/external quality assessment materials were analyzed by participating laboratories that used either immunoassay or LC-MS methods for total 25(OH)D...
August 10, 2017: Journal of AOAC International
Alison R Turnbull, Jessica Y C Tan, Sarah C Ugalde, Gustaaf M Hallegraeff, Katrina Campbell, D Tim Harwood, Juan José Dorantes-Aranda
Detection of paralytic shellfish toxins (PSTs) in bivalve shellfish by analytical methods is complicated and costly, requiring specific expertise and equipment. Following extensive blooms of Alexandrium tamarense Group 1 in Tasmania, Australia, an investigation was made into commercially available screening test kits suitable for use with the toxin profiles found in affected bivalves. The qualitative Neogen rapid test kit, with a modified protocol to convert gonyautoxins GTX1&4 and GTX2&3 into neosaxitoxin and saxitoxin (STX), respectively, with higher cross-reactivities, was the best fit-for-purpose...
August 10, 2017: Journal of AOAC International
Jennifer M Hait, Reginald W Bennett, Steven R Monday
Novel staphylococcal enterotoxins (SEs) expressed by Staphylococcus aureus strains have been described throughout the years, among these being the SE protein SER. To further characterize this toxin, this research used 13 S. aureus strains previously determined to contain the SE type R (ser) gene. These S. aureus isolates were evaluated using serological assays for identification of SEA–SEE and PCR for the detection of newly described SE and SE-like enterotoxin genes seg–seu. PCR-based cloning was performed such that the ser gene could be ligated into the pTrc99A plasmid expression vector...
August 8, 2017: Journal of AOAC International
Bixia Yang, Lian Wang, Chunying Luo, Xixi Wang, Chengjun Sun
An analytical method was developed for the simultaneous determination of 11 aminoglycoside (AG) antibiotics, including amikacin, paromomycin, dihydrostreptomycin, gentamicin C1a, hygromycin, kanamycin, netilmicin, spectinomycin, sisomicin, streptomycin, and tobramycin in honey, milk, and pork samples by LC with tandem MS and molecularly imprinted polymer (MIP) SPE. The AG antibiotics in milk and homogenated meat samples were extracted with a solution composed of 10 mmol/L potassium dihydrogen phosphate, 0.4 mmol/L EDTA-Na₂, and 2% trichloroacetic acid...
August 8, 2017: Journal of AOAC International
Brendon D Gill, Harvey E Indyk
A multilaboratory testing study was conducted on AOAC First Action Method 2016.05 “Analysis of Vitamin D2and Vitamin D3in Fortified Milk Powders, Infant Formulas, and Adult/Pediatric Nutritional Formulas by Liquid Chromatography-Tandem Mass Spectrometry.” Nine laboratories participated in the analysis of duplicate samples of 20 nutritional products. The samples were saponified at high temperature with lipid-soluble components extracted into isooctane; an aliquot was washed and vitamin D derivatized with 4-phenyl-1,2,4-triazoline-3,5-dione to form a high-molecular mass, easily ionizable adduct, extracted into acetonitrile and analyzed by reversed-phase LC-tandem MS...
August 8, 2017: Journal of AOAC International
Melissa Phillips, Håkan Emteborg
No abstract text is available yet for this article.
August 4, 2017: Journal of AOAC International
Danielly da Fonte Carvalho Martins, Lorena Coimbra Florindo, Anna Karolina Mouzer da Silva Machado, Vítor Todeschini, Maximiliano da Silva Sangoi
This study presents the development and validation of UV spectrophotometric methods for the determination of pinaverium bromide (PB) in tablet assay and dissolution studies. The methods were satisfactorily validated according to International Conference on Harmonization guidelines. The response was linear (r² > 0.99) in the concentration ranges of 2-14 μg/mL at 213 nm and 10-70 μg/mL at 243 nm. The LOD and LOQ were 0.39 and 1.31 μg/mL, respectively, at 213 nm. For the 243 nm method, the LOD and LOQ were 2...
August 4, 2017: Journal of AOAC International
Stephen Brooks, Linda Greene-Finestone, Susan Whiting, Vitali Fioletov, Patrick Laffey, Nicholas Petronella
Vitamin D status was assessed in 19–79 year old whites (8351 participants of European ancestry) and non-whites (1840 participants encompassing all other ancestries) from cycles 1 to 3 (years 2007–2013) of the Canadian Health Measures Survey. Status was assessed using the U.S. Institute of Medicine (IOM) 25-hydroxyvitamin D [25(OH)D] cut point values of 30 and 40 nmol/L. Overall, median 25(OH)D concentrations were significantly higher in whites [58.9 (28.6, 100.1) nmol/L; 5th and 95th percentile] compared with non-whites [43...
August 4, 2017: Journal of AOAC International
Nuket Kartal Temel, Ramazan Gürkan
In this study, a new ultrasonic-assisted cloud point extraction method coupled with spectrophotometry was developed to extract from and determine bisphenol A (BPA) in a sample matrix. The method is based on charge transfer-sensitive ion-pairing complex formation between BPA and Safranin T in the presence of nitrate in a micellar interface at pH 5.0. The variables affecting extraction efficiency were optimized. A good linear relationship with a significant sensitivity difference was obtained in the ranges of 2-40 and 2-120 µg/L with LODs of 0...
August 2, 2017: Journal of AOAC International
Guillermo Montero, Germán Günther, Karina Valdés, Francisco Arriagada, Javier Morales
In this work, we developed an HPLC method to simultaneously quantify and hence evaluate the stability, distribution, and antioxidant capacity of six isoflavones: genistein, genistin, daidzein, daidzin, glycitin, and biochanin A. Isoflavones have been described as having an important estrogenic activity to treat menopausal symptoms and can reduce postmenopausal bone loss and also participate in the prevention of cardiovascular diseases. These beneficial properties are believed derived from their capacity to act as free-radical scavengers...
August 2, 2017: Journal of AOAC International
Ahmed S Saad, Ibrahim A Naguib, Mohammed E Draz, Hala E Zaazaa, Adel S Lashien
Quantitative multicomponent analysis is considered an analytical goal to save time and cost in analysis. Hence, this work aimed to provide sensitive and selective UV-spectrophotometric, chemometric manipulation, and ultra-performance LC (UPLC) methods for the determination of well-known coformulated antiemetics used in pregnancy, namely pyridoxine HCl (PYR), meclozine HCl, and cyclizine. The developed UV-spectrophotometric methods are dual wavelength in ratio spectra and first derivative of the ratio spectra with which PYR was determined selectively at 290...
August 1, 2017: Journal of AOAC International
Camila Prado Cenciani de Souza, Cleide Aparecida de Abreu, Aline Renée Coscione, Cristiano Alberto de Andrade, Luiz Antonio Junqueira Teixeira, Flavia Consolini
Rapid, accurate, and low-cost alternative analytical methods for micronutrient quantification in fertilizers are fundamental in QC. The purpose of this study was to evaluate whether zinc (Zn) and copper (Cu) content in mineral fertilizers and industrial by-products determined by the alternative methods USEPA 3051a, 10% HCl, and 10% H₂SO₄ are statistically equivalent to the standard method, consisting of hot-plate digestion using concentrated HCl. The commercially marketed Zn and Cu sources in Brazil consisted of oxides, carbonate, and sulfate fertilizers and by-products consisting of galvanizing ash, galvanizing sludge, brass ash, and brass or scrap slag...
August 1, 2017: Journal of AOAC International
Duc Doan Nguyen, Francesco Busetti, Stuart Keith Johnson, Vicky Ann Solah
This study investigated beta-casomorphin 7 (BCM7) in yogurt by means of LC-tandem MS (MS/MS) and enzyme-linkedimmunosorbent assay (ELISA) and use LC-MS/MS as the "gold standard" method to evaluate the applicability of a commercial ELISA. The level of BCM7 in milk obtained from ELISA analysis was much lower than that obtained by LC-MS/MS analysis and trended to increase during fermentation and storage of yogurt. Meanwhile, the results obtained from LC-MS/MS showed that BCM7 degraded during stages of yogurt processing, and its degradation may have been caused by X-prolyl dipeptidyl aminopeptidase activity...
August 1, 2017: Journal of AOAC International
Silvia Tampucci, Eleonora Terreni, Susi Burgalassi, Patrizia Chetoni, Daniela Monti
A selective and rapid reversed-phase HPLC-UV method was developed and validated to quantify tavaborole (TAV; AN2690) in biological samples, i.e., in receiving phase and in bovine hoof membrane extract derived from in vitro transungual permeation studies. A simple solid-liquid extraction procedure was used to recover the drug from the bovine hoof slices. TAV chromatographic separation was achieved on a Luna PFP column (150 × 4.6 mm, 5 μm) using a mobile phase consisting of a 70% phosphoric acid solution (10 mM, pH 2...
August 1, 2017: Journal of AOAC International
Azza A Moustafa, Maha A Hegazy, Dalia Mohamed, Omnia Ali
The presence of coloring matters in syrups usually interferes with the spectrophotometric determination of active pharmaceutical ingredients. A novel approach was introduced to eliminate the interference of sunset yellow (coloring matter) in Cyrinol syrup. Smart, simple, accurate, and selective spectrophotometric methods were developed and validated for the simultaneous determination of a ternary mixture of carbinoxamine maleate, pholcodine, and ephedrine hydrochloride in syrup. Four of the applied methods used ratio spectra: successive derivative subtraction coupled with constant multiplication, successive derivative of ratio spectra, ratio subtraction coupled with ratio difference, and ratio spectra continuous wavelet transforms zero-crossing...
August 1, 2017: Journal of AOAC International
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