Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham
Channel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%...
July 19, 2023: Transgenic Research