journal
https://read.qxmd.com/read/38461212/establishment-of-a-hydrodynamic-delivery-system-in-ducks
#1
JOURNAL ARTICLE
Zhanji Zhao, Jiabing Zhu, Lijian Zhou, Nan Sun, Kaile Chang, Xiaoyue Hu, Yuting Hu, Mingzhi Ren, Yan Cheng, Derong Xu, Hongbo Xin, Chunbo Zhang
Chronic hepatitis B virus (HBV) poses a significant global health challenge as it can lead to acute or chronic liver disease and hepatocellular carcinoma (HCC). To establish a safety experimental model, a homolog of HBV-duck HBV (DHBV) is often used for HBV research. Hydrodynamic-based gene delivery (HGD) is an efficient method to introduce exogenous genes into the liver, making it suitable for basic research. In this study, a duck HGD system was first constructed by injecting the reporter plasmid pLIVE-SEAP via the ankle vein...
March 9, 2024: Transgenic Research
https://read.qxmd.com/read/38451380/specific-activity-and-utility-of-recombinant-cellobiohydrolase-ii-cel6a-produced-in-maize-endosperm
#2
JOURNAL ARTICLE
Enio Duque Y Duque, Milena Aguirre, Nathan C Hood, Elizabeth E Hood
Cellobiohydrolase II (CBH II) is an exo-glucanase that is part of a fungal mixture of enzymes from a wood-rot fungus, Trichoderma reesei. It is therefore difficult to purify and to establish a specific activity assay. The gene for this enzyme, driven by the rice Os glutelin promoter, was transformed into High II tissue culture competent corn, and the enzyme accumulated in the endosperm of the seed. The transgenic line recovered from tissue culture was bred into male and female elite Stine inbred corn lines, stiff stalk 16083-025 (female) and Lancaster MSO411 (male), for future production in their hybrid...
March 7, 2024: Transgenic Research
https://read.qxmd.com/read/38071732/efficiency-of-the-alpha-hairpinin-smamp-x-gene-promoter-from-stellaria-media-plant-depends-on-selection-of-transgenic-approach
#3
JOURNAL ARTICLE
Lyubov A Ivanova, Roman A Komakhin
The antimicrobial activity of the alpha-HAIRPININ ANTIMICROBIAL PEPTIDE X (SmAMP-X gene, GenBank acc. No. HG423454.1) from Stellaria media plant has been shown in vitro. Here, we isolated the SmAMP-X gene promoter and found two genomic sequences for the promoter (designated pro-SmAMP-X and pro-SmAMP-X-Ψ2) with 83% identity in their core and proximal regions. We found that the abilities of these promoters to express the uidA reporter and the nptII selectable marker differ according to the structural organization of T-DNA in the binary vector used for plant transformation...
December 10, 2023: Transgenic Research
https://read.qxmd.com/read/37851307/overexpressing-plant-ferredoxin-like-protein-enhances-photosynthetic-efficiency-and-carbohydrates-accumulation-in-phalaenopsis
#4
JOURNAL ARTICLE
Hsiang Chang, Yen-Ting Chen, Hsiang-En Huang, Mang-Jye Ger
Crassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron transport chain (ETC)...
December 2023: Transgenic Research
https://read.qxmd.com/read/37917348/abstracts-of-the-18th-transgenic-technology-meeting-tt2023-houston-texas-usa-november-12-15-2023
#5
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
November 2, 2023: Transgenic Research
https://read.qxmd.com/read/37874448/usefulness-of-current-sgrna-design-guidelines-and-in-vitro-cleavage-assays-for-plant-crispr-cas-genome-editing-a-case-targeting-the-polyphenol-oxidase-gene-family-in-eggplant-solanum-melongena-l
#6
JOURNAL ARTICLE
Mark Gabriel S Sagarbarria, John Albert M Caraan, Angelo John G Layos
The advent of genome editing platforms such as the CRISPR/Cas9 system ushers an unprecedented speed in the development of new crop varieties that can withstand the agricultural challenges of the 21st century. The CRISPR/Cas9 system depends on the specificity of engineered single guide RNAs (sgRNAs). However, sgRNA design in plants can be challenging due to the multitude of design tools to choose from, many of which use guidelines that are based on animal experiments yet allow the use of plant genomes. Upon choosing sgRNAs, it is also unclear whether an in vitro assay is needed to validate the targeting efficiency of a particular sgRNA before in vivo delivery of the CRISPR/Cas9 system...
October 24, 2023: Transgenic Research
https://read.qxmd.com/read/37851308/knockout-of-m-lp-mpv17l-a-newly-identified-atypical-pde-induces-physiological-afferent-cardiac-hypertrophy-in-mice
#7
JOURNAL ARTICLE
Reiko Iida, Misuzu Ueki, Toshihiro Yasuda
M-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricular lumen and thickening of the ventricular wall...
October 18, 2023: Transgenic Research
https://read.qxmd.com/read/37847464/generation-and-validation-of-a-myoglobin-knockout-zebrafish-model
#8
JOURNAL ARTICLE
Rasmus Hejlesen, Kasper Kjær-Sørensen, Angela Fago, Claus Oxvig
Previous studies using myoglobin (Mb) knockout mice and knockdown zebrafish have presented conflicting results about in vivo phenotypes resulting from the loss of this conserved and highly expressed protein, and therefore a new well-characterized knockout model is warranted. We here describe the generation of three distinct zebrafish mb knockout lines using the CRISPR/Cas system. None of the three lines exhibited any morphological phenotypes, changes in length, or lethality during embryonic and larval development...
October 17, 2023: Transgenic Research
https://read.qxmd.com/read/37843753/a-timely-user-friendly-and-flexible-marker-assisted-speed-congenics-method
#9
JOURNAL ARTICLE
Anne-Sophie Van Laere, Audrey Tromme, Laetitia Delaval, Frédéric Farnir, Joël Blomet, Daniel Desmecht
Mice are the most widely used mammalian animal model worldwide. Their use presents many advantages, including our ability to manipulate their genome. Unfortunately, transgenic mice often need to be introgressed to transfer the transgene of interest in a specific mouse line. This time-consuming process can be shortened using the speed congenics technique. However, the need for a panel of informative markers to evaluate the proportion of donor and receiver genomes in different individuals produced at each generation hinders the utilisation of speed congenics...
October 16, 2023: Transgenic Research
https://read.qxmd.com/read/37733197/in-vivo-crispr-lbcas12a-mediated-knock-in-and-knock-out-in-atlantic-salmon-salmo-salar-l
#10
JOURNAL ARTICLE
Mari Raudstein, Erik Kjærner-Semb, Morten Barvik, Silje Broll, Anne Hege Straume, Rolf Brudvik Edvardsen
Genome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigmentation gene solute carrier family 45 member 2 (slc45a2)...
September 21, 2023: Transgenic Research
https://read.qxmd.com/read/37707659/engineering-of-insecticidal-hybrid-gene-into-potato-chloroplast-genome-exhibits-promising-control-of-colorado-potato-beetle-leptinotarsa-decemlineata-coleoptera-chrysomelidae
#11
JOURNAL ARTICLE
Md Jakir Hossain, Allah Bakhsh, Faiz Ahmad Joyia, Emre Aksoy, Neslihan Zahide Özturk Gökçe, Muhammad Sarwar Khan
The potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85...
September 14, 2023: Transgenic Research
https://read.qxmd.com/read/37702987/efficient-transformation-and-regeneration-of-transgenic-plants-in-commercial-cultivars-of-citrus-aurantifolia-and-citrus-sinensis
#12
JOURNAL ARTICLE
Sweta Singh, Zeba Tarannum, Sunil Kokane, Dilip K Ghosh, Ashwani K Sharma, Harsh Chauhan
Citrus is one of the major horticultural crops with high economic and nutraceutical value. Despite the fact that conventional research has developed numerous improved varieties, citriculture is still susceptible to various stresses and requires innovative solutions such as genetic engineering. Among all the currently available modern approaches, Agrobacterium-mediated transformation is the most efficient method for introducing desired traits in citrus. However, being a non-host for Agrobacterium, various citrus species, including Citrus aurantifolia and Citrus sinensis, are recalcitrant to this method...
September 13, 2023: Transgenic Research
https://read.qxmd.com/read/37656262/assessment-of-co-infection-with-bnyvv-and-bsctv-on-resistance-against-rhizomania-disease-in-transgenic-sugar-beet-plants
#13
JOURNAL ARTICLE
Maryam Khoshnami, Bahar Zare, Hamideh Mardani-Mehrabad, Farshad Rakhshandehroo, Mohammad Amin Baghery, Mohammad Ali Malboobi
Sugar beet is an economically important crop and one of the major sources of sucrose around the world. Beet necrotic yellow vein virus (BNYVV) and Beet severe curly top virus (BSCTV) are two widespread viruses in sugar beet that cause severe damage to its performance. Previously, we have successfully produced resistance to BNYVV based on RNA silencing in sugar beet by introducing constructs carrying the viral coat-protein-encoding DNA sequence, CP21, in sense and anti-sense orientations. Yet, the RNA silencing-mediated resistance to a specific virus could be affected by other ones as a part of synergistic interactions...
September 1, 2023: Transgenic Research
https://read.qxmd.com/read/37615877/bioproduction-of-n-3-polyunsaturated-fatty-acids-by-nematode-fatty-acid-desaturases-and-elongase-in-drosophila-melanogaster
#14
JOURNAL ARTICLE
Mai Sato, Ryoma Ota, Satoru Kobayashi, Kimiko Yamakawa-Kobayashi, Takeshi Miura, Atsushi Ido, Yuya Ohhara
n-3 polyunsaturated fatty acids (n-3 PUFAs), including α-linolenic acid and eicosapentaenoic acid (EPA), are essential nutrients for vertebrates including humans. Vertebrates are n-3 PUFA-auxotrophic; hence, dietary intake of n-3 PUFAs is required for their normal physiology and development. Although fish meal and oil have been utilized as primary sources of n-3 PUFAs by humans and aquaculture, these traditional n-3 PUFA sources are expected to be exhausted because of the increasing consumption requirements of humans...
August 24, 2023: Transgenic Research
https://read.qxmd.com/read/37573273/diversity-of-transgenes-in-sustainable-management-of-insect-pests
#15
REVIEW
V Rakesh, Vinay K Kalia, Amalendu Ghosh
Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with the natural defenses developed by plants to counter insect attacks. Several toxin genes are also derived from spiders and scorpions for protection against insects. Bacillus thuringiensis Berliner is a microbial source of insecticidal toxins. Several methods have facilitated the large-scale production of transgenic plants...
August 12, 2023: Transgenic Research
https://read.qxmd.com/read/37540410/%C3%AE-1-3-galactosyltransferase-on-chromosome-6-is-essential-for-the-formation-of-lewis-a-structure-on-n-glycan-in-oryza-sativa
#16
JOURNAL ARTICLE
Jae-Wan Jung, Seong-Ryong Kim
β1,3-galactose is the component of outer-chain elongation of complex N-glycans that, together with α1,4-fucose, forms Lewis a  structures in plants. Previous studies have revealed that N-glycan maturation is mediated by sequential attachment of β1,3-galactose and α1,4-fucose by individual β1,3-galactosyltransferase (GalT) and α1,4-fucosyltransferase (1,4-FucT), respectively. Although GalT from several species has been studied, little information about GalT from rice is available...
August 4, 2023: Transgenic Research
https://read.qxmd.com/read/37535257/co-expression-of-four-penaeidins-in-transgenic-rice-seeds-an-alternative-strategy-for-substitute-antibiotic-agricultural-products
#17
JOURNAL ARTICLE
Xinyuan Song, Yu Qiao, Jian Ma, Xue Zhang, Jie Liu, Wen Xin, Shaochen Xing, Yunpeng Wang
The co-expression of multiple antimicrobial peptides (AMPs) in genetically modified (GM) crops can give plants a broader antibacterial spectrum and lower the pathogen risk of drug resistance. Therefore, four penaeidins (shrimp-derived AMPs) were fused and encoded in an artificial gene (PEN1234), driven by the seed-specific promoter Pzein, with the aim of co-expression in seeds of transgenic rice. The resistant rice plants, acquired via Agrobacterium-mediated transformation and glufosinate screening, were identified by PCR and the modified disk-diffusion method, and eight GM lines with high AMP content in the seeds were obtained...
August 3, 2023: Transgenic Research
https://read.qxmd.com/read/37468714/crispr-cas9-mediated-knock-in-of-masu-salmon-oncorhyncus-masou-elongase-gene-in-the-melanocortin-4-mc4r-coding-region-of-channel-catfish-ictalurus-punctatus-genome
#18
JOURNAL ARTICLE
Michael Coogan, De Xing, Baofeng Su, Veronica Alston, Andrew Johnson, Mohd Khan, Karim Khalil, Ahmed Elaswad, Shangjia Li, Jinhai Wang, Cuiyu Lu, Wenwen Wang, Darshika Hettiarachchi, Mei Shang, Tasnuba Hasin, Zhenkui Qin, Roger Cone, Ian A E Butts, Rex A Dunham
Channel catfish, Ictalurus punctatus, have limited ability to synthesize Ω-3 fatty acids. The ccβA-msElovl2 transgene containing masu salmon, Oncorhynchus masou, elongase gene driven by the common carp, Cyprinus carpio, β-actin promoter was inserted into the channel catfish melanocortin-4 receptor (mc4r) gene site using the two-hit two-oligo with plasmid (2H2OP) method. The best performing sgRNA resulted in a knockout mutation rate of 92%, a knock-in rate of 54% and a simultaneous knockout/knock-in rate of 49%...
July 19, 2023: Transgenic Research
https://read.qxmd.com/read/37415055/protein-familiarity-is-a-fundamental-but-rarely-operationalized-concept-in-the-safety-assessment-of-genetically-modified-crops-example-of-phosphomannose%C3%A2-isomerase-pmi
#19
JOURNAL ARTICLE
Anne B Carlson, Carey A Mathesius, Tim A Gunderson, Aideen Hession, Reba Bruyere, Henry P Mirsky, John Zhang, Mat Sandmann, Melissa N Fallers, Rod A Herman
Fundamental to the safety assessment of genetically modified (GM) crops is the concept of negligible risk for newly expressed proteins for which there is a history of safe use. Although this simple concept has been stated in international and regional guidance for assessing the risk of newly expressed proteins in GM crops, its full implementation by regulatory authorities has been lacking. As a result, safety studies are often repeated at a significant expenditure of resources by developers, study results are repeatedly reviewed by regulators, and animals are sacrificed needlessly to complete redundant animal toxicity studies...
July 6, 2023: Transgenic Research
https://read.qxmd.com/read/37351728/evaluation-of-cucumber-ubl5-promoter-as-a-tool-for-transgene-expression-and-genome-editing-in-plants
#20
JOURNAL ARTICLE
Kamonchanok Taway, Issariya Dachphun, Supachai Vuttipongchaikij, Anongpat Suttangkakul
Transgene expression and genome editing can help improve cucumber varieties to better respond to climate change. This study aimed to evaluate the applicability of the CsUBL5 promoter in transgene expression and genome editing in cucumber. The CsUBL5 promoter was cloned and analyzed to identify cis-elements that respond to abiotic signals, hormones, signal molecules, and nutrient treatments. 5' deletion constructs of the promoter were tested for their ability to drive GUS reporter expression in cucumber cotyledons, Arabidopsis seedlings, and tobacco leaves, and their response to various treatments including SA, light, drought, IAA, and GA was determined...
June 23, 2023: Transgenic Research
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