journal
MENU ▼
Read by QxMD icon Read
search

Transgenic Research

journal
https://www.readbyqxmd.com/read/30003470/constitutive-expression-of-antimicrobial-peptide-pr-39-in-transgenic-mice-significantly-enhances-resistance-to-bacterial-infection-and-promotes-growth
#1
Fang Zeng, Rui Dong, Chengcheng Zhao, Dewu Liu, Enqin Zheng, Changxu Song, Zhenfang Wu, Zicong Li
Use of huge amounts of antibiotics in farm animal production has promoted the prevalence of antibiotic-resistant bacteria, which poses a serious threat to public health. Therefore, alternative approaches are needed to reduce or replace antibiotic usage in the food animal industry. PR-39 is a pig-derived proline-rich antimicrobial peptide that has a broad spectrum of antibacterial activity and a low propensity for development of resistance by microorganisms. To test whether ubiquitous expression of PR-39 in transgenic (TG) mice can increase resistance against bacterial infection, we generated TG mice that ubiquitously express a pig-derived antimicrobial peptide PR-39 and analyzed their growth and resistance to infection of the highly pathogenic Actinobacillus pleuropneumoniae (APP) isolated from swine...
July 12, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29987710/gene-editing-the-phytoene-desaturase-alleles-of-cavendish-banana-using-crispr-cas9
#2
Fatima Naim, Benjamin Dugdale, Jennifer Kleidon, Anthony Brinin, Kylie Shand, Peter Waterhouse, James Dale
Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used the CRISPR/Cas9 gene editing system to deliver a self-cleaving polycistronic guide RNA (gRNA) designed to target exon 1 of the Phytoene desaturase (PDS) gene in the Cavendish cultivar "Williams"...
July 9, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29926349/production-of-functional-human-cuzn-sod-and-ec-sod-in-bitransgenic-cloned-goat-milk
#3
Rui Lu, Ting Zhang, Daijin Wu, Zhengyi He, Lei Jiang, Minya Zhou, Yong Cheng
Human copper/zinc superoxide dismutase (CuZn-SOD) and extracellular superoxide dismutase (EC-SOD) are two superoxide dismutases that scavenge reactive oxygen species (ROS). Their biological role of eliminating oxidative stress caused by excessive ROS levels in living organisms has been utilized in medical treatment, preventing skin photoaging and food preservation. In this study, we employed two sequences that encode human CuZn-SOD and EC-SOD, along with goat beta-casein 5' and 3' regulatory elements, to construct mammary gland-specific expression vectors...
June 20, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29876789/expression-of-disease-resistance-in-genetically-modified-grapevines-correlates-with-the-contents-of-viral-sequences-in-the-t-dna-and-global-genome-methylation
#4
Daniela Dal Bosco, Iraci Sinski, Patrícia S Ritschel, Umberto A Camargo, Thor V M Fajardo, Ricardo Harakava, Vera Quecini
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant's overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium-mediated transformation of somatic embryos and shoot apical meristems...
June 6, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29855762/unexpected-mutations-were-expected-and-unrelated-to-crispr-cas9-activity
#5
LETTER
Lluis Montoliu, C Bruce A Whitelaw
The scientific journal Nature Methods have just retracted a publication that reported numerous unexpected mutations after a CRISPR-Cas9 experiment based on collecting whole genome sequencing information from one control and two experimental genome edited mice. In the intervening 10 months since publication the data presented have been strongly contested and criticized by the scientific and biotech communities, through publications, open science channels and social networks. The criticism focused on the animal used as control, which was derived from the same mouse strain as the experimental individuals but from an unrelated sub-colony, hence control and experimental mice were genetically divergent...
May 31, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29797189/crispr-cas9-editing-of-carotenoid-genes-in-tomato
#6
Caterina D'Ambrosio, Adriana Lucia Stigliani, Giovanni Giorio
CRISPR/Cas9 technology is rapidly spreading as genome editing system in crop breeding. The efficacy of CRISPR/Cas9 in tomato was tested on Psy1 and CrtR-b2, two key genes of carotenoid biosynthesis. Carotenoids are plant secondary metabolites that must be present in the diet of higher animals because they exert irreplaceable functions in important physiological processes. Psy1 and CrtR-b2 were chosen because their impairment is easily detectable as a change of fruit or flower color. Two CRISPR/Cas9 constructs were designed to target neighboring sequences on the first exon of each gene...
May 24, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29777502/oxi1-kinase-plays-a-key-role-in-resistance-of-arabidopsis-towards-aphids-myzus-persicae
#7
Tahsin Shoala, Martin G Edwards, Marc R Knight, Angharad M R Gatehouse
Plants have co-evolved with a diverse array of pathogens and insect herbivores and so have evolved an extensive repertoire of constitutive and induced defence mechanisms activated through complex signalling pathways. OXI1 kinase is required for activation of mitogen-activated protein kinases (MAPKs) and is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses. Furthermore, changes in the levels of OXI1 appear to be crucial for appropriate signalling...
May 17, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29777501/expression-of-endogenous-proteins-in-maize-hybrids-in-a-multi-location-field-trial-in-india
#8
Linga R Gutha, Divakar Purushottam, Aruna Veeramachaneni, Sarita Tigulla, Vikas Kodappully, Chandana Enjala, Hitendrasinh Rajput, Jennifer Anderson, Bonnie Hong, Jean Schmidt, Shveta Bagga
Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference or housekeeping proteins are expected to maintain a relatively stable expression pattern in healthy plants given their role in cellular functions...
May 17, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29752697/biofortification-of-safflower-an-oil-seed-crop-engineered-for-ala-targeting-better-sustainability-and-plant-based-omega-3-fatty-acids
#9
Arti Rani, Asha Panwar, Manjary Sathe, Karunakara Alageri Chandrashekhara, Anil Kush
Alpha-linolenic acid (ALA) deficiency and a skewed n6:n3 fatty acid ratio in the diet is a major explanation for the prevalence of cardiovascular diseases and inflammatory/autoimmune diseases. There is mounting evidence of the health benefits associated with omega-3 long chain polyunsaturated fatty acids (LC PUFA's). Although present in abundance in fish, a number of factors limit our consumption of fish based omega-3 PUFA's. To name a few, overexploitation of wild fish stocks has reduced their sustainability due to increased demand of aquaculture for fish oil and meal; the pollution of marine food webs has raised concerns over the ingestion of toxic substances such as heavy metals and dioxins; vegetarians do not consider fish-based sources for supplemental nutrition...
May 11, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29728956/the-assessment-of-field-trials-in-gmo-research-around-the-world-and-their-possible-integration-in-field-trials-for-variety-registration
#10
REVIEW
M M Slot, C C M van de Wiel, G A Kleter, R G F Visser, E J Kok
Most regulations worldwide stipulate that a new genetically modified (GM) crop event has to be compared to its closest non-GM counterpart as a corner stone of the pre-market risk assessment. To this end the GM crop and its comparator should be grown in field trials for a phenotypic comparison as well as for subsequent detailed analysis of the composition of the two crop varieties. A more in-depth globally harmonised approach for the conduct of these field trials is lacking. Only a few countries have formulated detailed protocols for the set-up of GM field trials...
May 4, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29728958/comparative-compositional-analysis-of-transgenic-potato-resistant-to-potato-tuber-moth-ptm-and-its-non-transformed-counterpart
#11
Hassan Rahnama, Amir Bahram Moradi, Seyed Hamid Mirrokni, Foad Moradi, Mohammad Reza Shams, Mohammad Hossein Fotokian
In this study, the compositions of transgenic potatoes (TPs) resistant to potato tuber moth (Phthorimaea operculella) were compared with those of its non-transgenic (NTP) counterparts. The light inducible promoter, phosphoenolpyruvate carboxylase led to the expression of Cry1Ab only in the leaves and light-treated tubers of the TPs. No significant differences were found in the moisture, ash, dry weight, total soluble protein, carbohydrate, starch, fiber, ascorbate, cations, anions, fatty acids, and glycoalkaloids contents of TP and NTP...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29728957/over-expression-of-the-pseudomonas-syringae-harpin-encoding-gene-hrpzm-confers-enhanced-tolerance-to-phytophthora-root-and-stem-rot-in-transgenic-soybean
#12
Qian Du, Xiangdong Yang, Jinhua Zhang, Xiaofang Zhong, Kyung Seok Kim, Jing Yang, Guojie Xing, Xiaoyu Li, Zhaoyuan Jiang, Qiyun Li, Yingshan Dong, Hongyu Pan
Phytophthora root and stem rot (PRR) caused by Phytophthora sojae is one of the most devastating diseases reducing soybean (Glycine max) production all over the world. Harpin proteins in many plant pathogenic bacteria were confirmed to enhance disease and insect resistance in crop plants. Here, a harpin protein-encoding gene hrpZpsta from the P. syringae pv. tabaci strain Psta218 was codon-optimized (renamed hrpZm) and introduced into soybean cultivars Williams 82 and Shennong 9 by Agrobacterium-mediated transformation...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29691708/generation-of-insulin-deficient-piglets-by-disrupting-ins-gene-using-crispr-cas9-system
#13
Bumrae Cho, Su Jin Kim, Eun-Jin Lee, Sun Mi Ahn, Jin Seok Lee, Dal-Young Ji, Kiho Lee, Jung-Taek Kang
Diabetes mellitus is a chronic disease with accompanying severe complications. Various animal models, mostly rodents due to availability of genetically modified lines, have been used to investigate the pathophysiology of diabetes. Using pigs for diabetic research can be beneficial because of their similarity in size, pathogenesis pathway, physiology, and metabolism with human. However, the use of pigs for diabetes research has been hampered due to only few pig models presenting diabetes symptoms. In this study, we have successfully generated insulin-deficient pigs by generating the indels of the porcine INS gene in somatic cells using CRISPR/Cas9 system followed by somatic cell nuclear transfer...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29663254/novel-insights-into-the-genetic-background-of-genetically-modified-mice
#14
Peter Dobrowolski, Melina Fischer, Ronald Naumann
Unclear or misclassified genetic background of laboratory rodents or a lack of strain awareness causes a number of difficulties in performing or reproducing scientific experiments. Until now, genetic differentiation between strains and substrains of inbred mice has been a challenge. We have developed a screening method for analyzing inbred strains regarding their genetic background. It is based on 240 highly informative short tandem repeat (STR) markers covering the 19 autosomes as well as X and Y chromosomes...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29651659/current-achievements-and-future-directions-in-genetic-engineering-of-european-plum-prunus-domestica-l
#15
REVIEW
Cesar Petri, Nuria Alburquerque, Mohamed Faize, Ralph Scorza, Chris Dardick
In most woody fruit species, transformation and regeneration are difficult. However, European plum (Prunus domestica) has been shown to be amenable to genetic improvement technologies from classical hybridization, to genetic engineering, to rapid cycle crop breeding ('FasTrack' breeding). Since the first report on European plum transformation with marker genes in the early 90 s, numerous manuscripts have been published reporting the generation of new clones with agronomically interesting traits, such as pests, diseases and/or abiotic stress resistance, shorter juvenile period, dwarfing, continuous flowering, etc...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29594927/differences-between-immunodeficient-mice-generated-by-classical-gene-targeting-and-crispr-cas9-mediated-gene-knockout
#16
Jae Hoon Lee, Jong-Hyung Park, Tae-Wook Nam, Sun-Min Seo, Jun-Young Kim, Han-Kyul Lee, Jong Hyun Han, Song Yi Park, Yang-Kyu Choi, Han-Woong Lee
Immunodeficient mice are widely used for pre-clinical studies to understand various human diseases. Here, we report the generation of four immunodeficient mouse models using CRISPR/Cas9 system without inserting any foreign gene sequences such as NeoR cassettes and their characterization. By eliminating any possible effects of adding a NeoR cassette, our mouse models may allow us to better elucidate the in vivo functions of each gene. Our FVB-Rag2-/- , B6-Rag2-/- , and BALB/c-Prkdc-/- mice showed phenotypes similar to those of the earlier immunodeficient mouse models, including a lack of mature B cells and T cells and an increase in the number of CD45+ DX-5+ natural killer cells...
June 2018: Transgenic Research
https://www.readbyqxmd.com/read/29574513/insights-into-phytase-containing-transgenic-lemna-minor-l-as-a-novel-feed-additive
#17
Mrinmoy Ghosh, Neelesh Sharma, Meeta Gera, Nameun Kim, Do Huynh, Jiaojiao Zhang, Taesun Min, Simrinder Singh Sodhi, Min Bae Kim, V P B Rekha, Sukmin Ko, Dong Kee Jeong
This study assessed the effect of supplementation of novel transgenic phytase on growth performance and bone mineralization in Korean native broiler chickens. The experiment was designed using four dietary groups: those with a diet supplemented with (A) recombinant phytase, (B) transgenic phytase from the plant Lemna minor, (C) or wild-type L. minor as well as (D) a control group that was supplemented with commercially available feed. Three hundred 1-day-old Korean native broiler chicks were used and divided into these four dietary treatment groups having three replicates of 25 birds each (n = 75)...
April 2018: Transgenic Research
https://www.readbyqxmd.com/read/29546522/novel-reporter-and-deleter-mouse-strains-generated-using-vcre-vloxp-and-scre-sloxp-systems-and-their-system-specificity-in-mice
#18
Yuki Yoshimura, Miyuki Ida-Tanaka, Tsuyoshi Hiramaki, Motohito Goto, Tsutomu Kamisako, Tomoo Eto, Mika Yagoto, Kenji Kawai, Takeshi Takahashi, Manabu Nakayama, Mamoru Ito
DNA site-specific recombination by Cre/loxP is a powerful tool for gene manipulation in experimental animals. VCre/VloxP and SCre/SloxP are novel site-specific recombination systems, consisting of a recombinase and its specific recognition sequences, which function in a manner similar to Cre/loxP. Previous reports using Escherichia coli and Oryzias latipes demonstrated the existence of stringent specificity between each recombinase and its target sites; VCre/VloxP, SCre/SloxP, and Cre/loxP have no cross-reactivity with each other...
April 2018: Transgenic Research
https://www.readbyqxmd.com/read/29516259/zygote-injection-of-rna-encoding-cre-recombinase-results-in-efficient-removal-of-loxp-flanked-neomycin-cassettes-in-pigs
#19
Kristin M Whitworth, Raissa Cecil, Joshua A Benne, Bethany K Redel, Lee D Spate, Melissa S Samuel, Randall S Prather, Kevin D Wells
Genetically engineered pigs are often created with a targeting vector that contains a loxP flanked selectable marker like neomycin. The Cre-loxP recombinase system can be used to remove the selectable marker gene from the resulting offspring or cell line. Here is described a new method to remove a loxP flanked neomycin cassette by direct zygote injection of an mRNA encoding Cre recombinase. The optimal concentration of mRNA was determined to be 10 ng/μL when compared to 2 and 100 ng/μL (P < 0.0001)...
April 2018: Transgenic Research
https://www.readbyqxmd.com/read/29492792/a-virus-derived-short-hairpin-rna-confers-resistance-against-sugarcane-mosaic-virus-in-transgenic-sugarcane
#20
Usman Aslam, Bushra Tabassum, Idrees Ahmad Nasir, Anwar Khan, Tayyab Husnain
RNA interference (RNAi) is commonly used to produce virus tolerant transgenic plants. The objective of the current study was to generate transgenic sugarcane plants expressing a short hairpin RNAs (shRNA) targeting the coat protein (CP) gene of sugarcane mosaic virus (SCMV). Based on multiple sequence alignment, including genomic sequences of four SCMV strains, a conserved region of ~ 456 bp coat protein (CP) gene was selected as target gene and amplified through polymerase chain reaction (PCR). Subsequently, siRNAs2 and siRNA4 were engineered as stable short hairpin (shRNA) transgenes of 110 bp with stem and loop sequences derived from microRNA (sof-MIR168a; an active regulatory miRNA in sugarcane)...
April 2018: Transgenic Research
journal
journal
31061
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"