journal
MENU ▼
Read by QxMD icon Read
search

Transgenic Research

journal
https://www.readbyqxmd.com/read/28432545/genome-edited-animals-learning-from-gm-crops
#1
Ann Bruce
Genome editing of livestock is poised to become commercial reality, yet questions remain as to appropriate regulation, potential impact on the industry sector and public acceptability of products. This paper looks at how genome editing of livestock has attempted to learn some of the lessons from commercialisation of GM crops, and takes a systemic approach to explore some of the complexity and ambiguity in incorporating genome edited animals in a food production system. Current applications of genome editing are considered, viewed from the perspective of past technological applications...
April 21, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28432544/expression-of-cphb-and-cphe-type-cyanophycinases-in-cyanophycin-producing-tobacco-and-comparison-of-their-ability-to-degrade-cyanophycin-in-plant-and-plant-extracts
#2
Daniel Ponndorf, Inge Broer, Henrik Nausch
Increasing the arginine (Arg) content in plants used as feed or food is of interest, since the supplementation of food with conditionally essential Arg has been shown to have nutritional benefits. An increase was achieved by the expression of the Arg-rich bacterial storage component, cyanophycin (CGP), in the chloroplast of transgenic plants. CGP is stable in plants and its degradation into β-aspartic acid (Asp)-Arg dipeptides, is solely catalyzed by bacterial cyanophycinases (CGPase). Dipeptides can be absorbed by animals even more efficiently than free amino acids (Matthews and Adibi 1976; Wenzel et al...
April 21, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28417275/rna-silencing-in-the-life-cycle-of-soybean-multiple-restriction-systems-and-spatiotemporal-variation-associated-with-plant-architecture
#3
Ayumi Mori, Hiroshi Sato, Megumi Kasai, Tetsuya Yamada, Akira Kanazawa
The expression of transgenes introduced into a plant genome is sometimes suppressed by RNA silencing. Although local and systemic spread of RNA silencing have been studied, little is known about the mechanisms underlying spatial and temporal variation in transgene silencing between individual plants or between plants of different generations, which occurs seemingly stochastically. Here, we analyzed the occurrence, spread, and transmission of RNA silencing of the green fluorescent protein (GFP) gene over multiple generations of the progeny of a single soybean transformant...
April 17, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28409408/spontaneous-recombinase-activity-of-cre-ert2-in-vivo
#4
Jasmin Kristianto, Michael G Johnson, Ryley K Zastrow, Abigail B Radcliff, Robert D Blank
Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre-ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT. The putative mechanism of selective Cre-mediated recombination is Cre sequestration in the cytoplasm in the basal state with translocation to the nucleus only in the presence of tamoxifen...
April 13, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28349287/growth-modulation-effects-of-cbm2a-under-the-control-of-atexp4-and-camv35s-promoters-in-arabidopsis-thaliana-nicotiana-tabacum-and-eucalyptus-camaldulensis
#5
Pornthep Keadtidumrongkul, Anongpat Suttangkakul, Phitsanu Pinmanee, Kanokwan Pattana, Chokchai Kittiwongwattana, Somsak Apisitwanich, Supachai Vuttipongchaikij
The expression of cell-wall-targeted Carbohydrate Binding Modules (CBMs) can alter cell wall properties and modulate growth and development in plants such as tobacco and potato. CBM2a identified in xylanase 10A from Cellulomonas fimi is of particular interest for its ability to bind crystalline cellulose. However, its potential for promoting plant growth has not been explored. In this work, we tested the ability of CBM2a to promote growth when expressed using both CaMV35S and a vascular tissue-specific promoter derived from Arabidopsis expansin4 (AtEXP4) in three plant species: Arabidopsis, Nicotiana tabacum and Eucalyptus camaldulensis...
March 27, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28332009/n-glycosylation-of-an-igg-antibody-secreted-by-nicotiana-tabacum-by-2-cells-can-be-modulated-through-co-expression-of-human-%C3%AE-1-4-galactosyltransferase
#6
Catherine Navarre, Nicolas Smargiasso, Laurent Duvivier, Joseph Nader, Johann Far, Edwin De Pauw, Marc Boutry
Nicotiana tabacum BY-2 suspension cells have several advantages that make them suitable for the production of full-size monoclonal antibodies which can be purified directly from the culture medium. Carbohydrate characterization of an antibody (Lo-BM2) expressed in N. tabacum BY-2 cells showed that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (>70%) of the complex GnGnXF glycoform. The stable co-expression of a human β-1,4-galactosyltransferase targeted to different Golgi sub-compartments altered Lo-BM2N-glycosylation and resulted in the production of an antibody that exhibited either hybrid structures containing a low abundance of the plant epitopes (α-1,3-fucose and β-1,2-xylose), or a large amount of galactose-extended N-glycan structures...
March 22, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28326506/influence-of-calcareous-soil-on-cry3bb1-expression-and-efficacy-in-the-field
#7
David S Wangila, Arnubio Valencia J, Haichuan Wang, Blair D Siegfried, Lance J Meinke
Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of unexpected injury, experiments were conducted during 2013 and 2014 to ascertain whether the calcareous soil conditions and associated poor maize growth negatively affect the expression of Cry3Bb1...
March 22, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28314980/ex-post-assessment-of-genetically-modified-low-level-presence-in-canadian-flax
#8
Helen M Booker, Eric G Lamb, Stuart J Smyth
Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax industry in Canada undertook major changes to ensure the removal of transgenic flax from the supply chain. To demonstrate compliance, Canada adopted a protocol involving testing grain samples (post-harvest) using an RT-PCR test for the construct found in CDC Triffid...
March 17, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28243835/a-lifetime-aging-study-of-human-cd19-transgenic-mice
#9
W Iverson, E Straley, S Oldham, J Rojko, S Turman, Y Wang
Mice transgenic for human CD19 have been an important animal model to help understand the role of this molecule in B lymphocyte function. Previously, no lifetime studies had been performed to understand the effects of this CD19 over expression on the survival or spontaneous pathology within the C57BL/6J background strain. We conducted a lifetime study with interim sacrifices to understand the transgenic effects on clinical signs, body weight, survival, and spontaneous pathology. Blood and urine samples were collected from select animals at various time points during the study for measurement of clinical pathology parameters and groups of animals were euthanized and examined at predetermined intervals...
February 27, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28220277/establishment-of-a-heteroplasmic-mouse-strain-with-interspecific-mitochondrial-dna-haplotypes-and-improvement-of-a-pcr-rflp-based-measurement-system-for-estimation-of-mitochondrial-dna-heteroplasmy
#10
Hiroshi Shitara, Liqin Cao, Midori Yamaguchi, Hiromichi Yonekawa, Choji Taya
Mitochondrial DNA segregation is one of the characteristic modes of mitochondrial inheritance in which the heteroplasmic state of mitochondrial DNA is transmitted to the next generation in variable proportions. To analyze mitochondrial DNA segregation, we produced a heteroplasmic mouse strain with interspecific mitochondrial DNA haplotypes, which contains two types of mitochondrial DNA derived from C57BL/6J and Mus spretus strains. The strain was produced on a C57BL/6J nuclear genomic background by microinjection of donor cytoplasm into fertilized eggs...
February 20, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28144770/genome-editing-and-the-future-of-farming-meeting-report
#11
Monica Hoyos-Flight, Emilie Brady, Helen Sang, Bruce Whitelaw
No abstract text is available yet for this article.
April 2017: Transgenic Research
https://www.readbyqxmd.com/read/27905063/efficient-gene-targeting-in-mouse-zygotes-mediated-by-crispr-cas9-protein
#12
Chris J Jung, Junli Zhang, Elizabeth Trenchard, Kent C Lloyd, David B West, Barry Rosen, Pieter J de Jong
The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We characterized several parameters, including single guide RNA cleavage activity and the length and symmetry of homology arms in the construct, and we compared the targeting efficiency between Cas9, Cas9nickase, and dCas9-FokI...
April 2017: Transgenic Research
https://www.readbyqxmd.com/read/27873161/fgf-treatment-of-host-embryos-injected-with-es-cells-increases-rates-of-chimaerism
#13
Cathérine Dupont, Friedemann Loos, John Kong-A-San, Joost Gribnau
In spite of the emergence of genome editing tools, ES cell mediated transgenesis remains the most controllable way of creating genetically modified animals. Although tetraploid (4N) complementation of 4N host embryos and ES cells, is the only method guaranteeing that offspring are entirely ES cell derived, this technique is challenging, not always successful and difficult to implement in some laboratory settings. The current study shows that pretreatment of host blastocysts with FGF4 prior to ES cell injection can provide an alternative method for the generation of animals displaying high rates of chimaerism...
April 2017: Transgenic Research
https://www.readbyqxmd.com/read/27838898/generation-of-mouse-lines-with-conditionally-or-constitutively-inactivated-snca-gene-and-rosa26-stop-lacz-reporter-located-in-cis-on-the-mouse-chromosome-6
#14
Andrei Yu Roman, Galina Limorenko, Alexey A Ustyugov, Tatiana V Tarasova, Ekaterina A Lysikova, Vladimir L Buchman, Natalia Ninkina
α-Synuclein is involved in many important molecular processes in neuronal cells and their synapses, and its malfunction has been linked to the development of Parkinson's and certain other neurodegenerative diseases. Animal models allowing tightly monitored conditional inactivation of the encoding gene, Snca, are indispensible for studies aimed at understanding normal function of α-synuclein in various neuronal populations and its role in pathogenesis of neurodegenerative diseases. We have recently reported the production of several novel mouse lines for manipulating expression of the endogenous Snca gene, including a line for Cre-recombinase-driven conditional inactivation of the gene (mice with floxed Snca) and a new line with a constitutive knockout of α-synuclein...
April 2017: Transgenic Research
https://www.readbyqxmd.com/read/27832434/monitoring-of-venus-transgenic-cell-migration-during-pregnancy-in-non-transgenic-rabbits
#15
N Lipták, O I Hoffmann, A Kerekes, G Iski, D Ernszt, K Kvell, L Hiripi, Z Bősze
Cell transfer between mother and fetus were demonstrated previously in several species which possess haemochorial placenta (e.g. in humans, mice, rats, etc.). Here we report the assessment of fetal and maternal microchimerism in non-transgenic (non-TG) New Zealand white rabbits which were pregnant with transgenic (TG) fetuses and in non-TG newborns of TG does. The TG construct, including the Venus fluorophore cDNA driven by a ubiquitous cytomegalovirus enhancer, chicken ß-actin promoter (CAGGS), was previously integrated into the rabbit genome by Sleeping Beauty transposon system...
April 2017: Transgenic Research
https://www.readbyqxmd.com/read/27995503/improvement-of-anti-nutritional-effect-resulting-from-%C3%AE-glucanase-specific-expression-in-the-parotid-gland-of-transgenic-pigs
#16
Li-Zeng Guan, Jin-Shun Cai, Shuai Zhao, Yu-Ping Sun, Jing-Lan Wang, Yong Jiang, Gang Shu, Qing-Yan Jiang, Zhen-Fang Wu, Qian-Yun Xi, Yong-Liang Zhang
β-Glucan is the predominant anti-nutritional factors in monogastric animal feed. Although β-glucanase supplementation in diet can help to eliminate the adverse effects, enzyme stability is substantially modified during the feed manufacturing process. To determine whether the expression of endogenous β-glucanase gene (GLU) in vivo can improve digestibility of dietary β-glucan and absorption of nutrients, we successfully produced transgenic pigs via nuclear transfer which express the GLU from Paenibacillus polymyxa CP7 in the parotid gland...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27900536/t%C3%AE-4-overexpression-based-on-the-piggybac-transposon-system-in-cashmere-goats-alters-hair-fiber-characteristics
#17
Bingbo Shi, Qiang Ding, Xiaolin He, Haijing Zhu, Yiyuan Niu, Bei Cai, Jiao Cai, Anming Lei, Danju Kang, Hailong Yan, Baohua Ma, Xiaolong Wang, Lei Qu, Yulin Chen
Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (Tβ4) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (KAP6.1) promoter...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27787698/the-transgenic-expression-of-human-follistatin-344-increases-skeletal-muscle-mass-in-pigs
#18
Fei Chang, Rui Fang, Meng Wang, Xin Zhao, Wen Chang, Zaihu Zhang, Ning Li, Qingyong Meng
Follistatin (FST), which was first found in the follicles of cattle and pigs, has been shown to be an essential regulator for muscle development. Mice that were genetically engineered to overexpress Fst specifically in muscle had at least twice the amount of skeletal muscle mass as controls; these findings are similar to earlier results obtained in myostatin-knockout mice. However, the role of follistatin in skeletal muscle development has yet to be clarified in livestock. Here, we describe transgenic Duroc pigs that exogenously express Fst specifically in muscle tissue...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27778153/phenotypic-characterization-of-a-novel-ho-1-depletion-model-in-the-rat
#19
Vassilios Atsaves, Maria G Detsika, Elpida Poulaki, Hara Gakiopoulou, Elias A Lianos
Although the protective role of HO-1 induction in various forms of kidney disease is well established, mechanisms other than heme catabolism to biliverdin, bilirubin and carbon monoxide have recently been identified. Unraveling these mechanisms requires the generation of appropriate animal models. The present study describes the generation of a HO-1 deficient Hmox1 (-/-) rat model and characterizes its renal and extrarenal phenotype. Hmox1 (-/-) rats had growth retardation and splenomegaly compared to their Hmox1 (+/+) littermates...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27771867/plant-characterization-of-genetically-modified-maize-hybrids-mon-89%C3%A3-34-3%C3%A2-%C3%A3-%C3%A2-mon-88%C3%A3-17-3-mon-89%C3%A3-34-3%C3%A2-%C3%A3-%C3%A2-mon-%C3%A3-%C3%A3-6%C3%A3-3-6-and-mon-%C3%A3-%C3%A3-6%C3%A3-3-6-alternatives-for-maize-production-in-mexico
#20
Oscar Heredia Díaz, José Luis Aldaba Meza, Baltazar M Baltazar, Germán Bojórquez Bojórquez, Luciano Castro Espinoza, José Luis Corrales Madrid, Juan Manuel de la Fuente Martínez, Héctor Abel Durán Pompa, José Alonso Escobedo, Armando Espinoza Banda, José Antonio Garzón Tiznado, Juvencio González García, José Luis Guzmán Rodríguez, Jesús Ignacio Madueño Martínez, José Luis Martínez Carrillo, Chen Meng, Francisco Javier Quiñones Pando, Enrique Rosales Robles, Ignacio Ruiz Hernández, José Elías Treviño Ramírez, Hugo Raúl Uribe Montes, Francisco Zavala García
Environmental risk assessment (ERA) of genetically modified (GM) crops is a process to evaluate whether the biotechnology trait(s) in a GM crop may result in increased pest potential or harm to the environment. In this analysis, two GM insect-resistant (IR) herbicide-tolerant maize hybrids (MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and one herbicide-tolerant GM hybrid (MON-ØØ6Ø3-6) were compared with conventional maize hybrids of similar genetic backgrounds. Two sets of studies, Experimental Phase and Pilot Phase, were conducted across five ecological regions (ecoregions) in Mexico during 2009-2013, and data were subject to meta-analysis...
February 2017: Transgenic Research
journal
journal
31061
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"