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Transgenic Research

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https://www.readbyqxmd.com/read/29143178/transgenic-cotton-co-expressing-chimeric-vip3acaa-and-cry1ac-confers-effective-protection-against-cry1ac-resistant-cotton-bollworm
#1
Wen-Bo Chen, Guo-Qing Lu, Hong-Mei Cheng, Chen-Xi Liu, Yu-Tao Xiao, Chao Xu, Zhi-Cheng Shen, Mario Soberón, Alejandra Bravo, Kong-Ming Wu
Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (Helicoverpa armigera) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by H. armigera remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three H...
November 15, 2017: Transgenic Research
https://www.readbyqxmd.com/read/29094286/genome-editing-in-livestock-are-we-ready-for-a-revolution-in-animal-breeding-industry
#2
REVIEW
Jinxue Ruan, Jie Xu, Ruby Yanru Chen-Tsai, Kui Li
Genome editing is a powerful technology that can efficiently alter the genome of organisms to achieve targeted modification of endogenous genes and targeted integration of exogenous genes. Current genome-editing tools mainly include ZFN, TALEN and CRISPR/Cas9, which have been successfully applied to all species tested including zebrafish, humans, mice, rats, monkeys, pigs, cattle, sheep, goats and others. The application of genome editing has quickly swept through the entire biomedical field, including livestock breeding...
November 1, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28993973/crispr-cas9-mediated-knockout-of-myostatin-in-chinese-indigenous-erhualian-pigs
#3
Kankan Wang, Xiaochun Tang, Zicong Xie, Xiaodong Zou, Mengjing Li, Hongming Yuan, Nannan Guo, Hongsheng Ouyang, Huping Jiao, Daxin Pang
CRISPR/Cas9 has emerged as one of the most popular genome editing tools due to its simple design and high efficiency in multiple species. Myostatin (MSTN) negatively regulates skeletal muscle growth and mutations in myostatin cause double-muscled phenotype in various animals. Here, we generated myostatin mutation in Erhualian pigs using a combination of CRISPR/Cas9 and somatic cell nuclear transfer. The protein level of myostatin precursor decreased dramatically in mutant cloned piglets. Unlike myostatin knockout Landrace, which often encountered health issues and died shortly after birth, Erhualian pigs harboring homozygous mutations were viable...
October 9, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28986672/high-accumulation-in-tobacco-seeds-of-hemagglutinin-antigen-from-avian-h5n1-influenza
#4
Yanaysi Ceballo, Kenia Tiel, Alina López, Gleysin Cabrera, Marlene Pérez, Osmany Ramos, Yamilka Rosabal, Carlos Montero, Rima Menassa, Ann Depicker, Abel Hernández
Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes...
October 6, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28952064/effects-of-transgenic-overexpression-of-diapause-hormone-and-diapause-hormone-receptor-genes-on-non-diapause-silkworm
#5
Chunying Gong, Wenhui Zeng, Tianyang Zhang, Rongpeng Liu, Yao Ou, Junwen Ai, Zhonghuai Xiang, Hanfu Xu
Diapause is a state of developmental arrest that is most often observed in arthropods, especially insects. The domesticated silkworm, Bombyx mori, is a typical insect that enters diapause at an early embryonic stage. Previous studies have revealed that the diapause hormone (DH) signaling molecules, especially the core members DH and DH receptor 1 (DHR1), are crucial for the determination of embryonic diapause in diapause silkworm strains. However, whether they function in non-diapause silkworm strains remains largely unknown...
September 26, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28940087/expression-of-a-fungal-laccase-fused-with-a-bacterial-cellulose-binding-module-improves-the-enzymatic-saccharification-efficiency-of-lignocellulose-biomass-in-transgenic-arabidopsis-thaliana
#6
Ryota Iiyoshi, Taichi Oguchi, Toru Furukawa, Yosuke Iimura, Yukihiro Ito, Tomonori Sonoki
Delignification is effective for improving the saccharification efficiency of lignocellulosic biomass materials. We previously identified that the expression of a fungal laccase (Lac) fused with a bacterial cellulose-binding module domain (CBD) improved the enzymatic saccharification efficiency of rice plants. In this work, to evaluate the ability of the Lac-CBD fused chimeric enzyme to improve saccharification efficiency in a dicot plant, we introduced the chimeric gene into a dicot model plant, Arabidopsis thaliana...
September 22, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28916981/expression-of-a-novel-bi-directional-brassica-napus-promoter-in-soybean
#7
Siva Chennareddy, Toby Cicak, Lauren Clark, Sean Russell, Michiyo Skokut, Jeffrey Beringer, Xiaozeng Yang, Yi Jia, Manju Gupta
The expression profile of a natural bi-directional promoter, derived from the Brassica napus EPSPS-A gene, was studied in transgenic soybean (Glycine max C.V. Maverick) lines. Two constructs, pDAB100331 and pDAB100333, were assembled to test the bi-directionality of the promoter. Two reporter genes, gfp and gusA, were employed and they were interchangeably placed in both constructs, one on each end of the promoter such that both proteins expressed divergently in each construct. In the T0 generation, GUS expression was more uniform throughout the leaf of pDAB100333 transgenic plants, where the gusA gene was expressed from the downstream or EPSPS-A end of the bi-directional promoter...
September 15, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28840434/robust-rnai-mediated-resistance-to-infection-of-seven-potyvirids-in-soybean-expressing-an-intron-hairpin-nib-rna
#8
Xiangdong Yang, Lu Niu, Wei Zhang, Hongli He, Jing Yang, Guojie Xing, Dongquan Guo, Qian Du, Xueyan Qian, Yao Yao, Qiyun Li, Yingshan Dong
Viral pathogens, such as soybean mosaic virus (SMV), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. Engineering resistance by RNAi-mediated gene silencing is a powerful strategy for controlling viral diseases. In this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, NIb) gene was isolated from the SMV SC3 strain, driven by the leaf-specific rbcS2 promoter from Phaseolus vulgaris, and introduced into soybean. The transgenic lines had significantly lower average disease indices (ranging from 2...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28801873/transgenic-silkworms-secrete-the-recombinant-glycosylated-mrjp1-protein-of-chinese-honeybee-apis-cerana-cerana
#9
Zhengying You, Qiujie Qian, Yiran Wang, Jiaqian Che, Lupeng Ye, Lirong Shen, Boxiong Zhong
Major royal jelly protein-1 (MRJP1) is the most abundant glycoprotein of royal jelly (RJ) and is considered a potential component of functional foods. In this study, we used silkworm transgenic technology to obtain five transgenic silkworm lineages expressing the exogenous recombinant Chinese honeybee, Apis cerana cerana, protein-1 (rAccMRJP1) under the control of a fibroin light chain (Fib-L) promoter in the posterior silk glands. The protein was successfully secreted into cocoons; specifically, the highest rAccMRJP1 protein content was 0...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28780744/advances-in-transgenic-animal-models-and-techniques
#10
Séverine Ménoret, Laurent Tesson, Séverine Remy, Claire Usal, Laure-Hélène Ouisse, Lucas Brusselle, Vanessa Chenouard, Ignacio Anegon
On May 11th and 12th 2017 was held in Nantes, France, the international meeting "Advances in transgenic animal models and techniques" ( http://www.trm.univ-nantes.fr/ ). This biennial meeting is the fifth one of its kind to be organized by the Transgenic Rats ImmunoPhenomic (TRIP) Nantes facility ( http://www.tgr.nantes.inserm.fr/ ). The meeting was supported by private companies (SONIDEL, Scionics computer innovation, New England Biolabs, MERCK, genOway, Journal Disease Models and Mechanisms) and by public institutions (International Society for Transgenic Technology, University of Nantes, INSERM UMR 1064, SFR François Bonamy, CNRS, Région Pays de la Loire, Biogenouest, TEFOR infrastructure, ITUN, IHU-CESTI and DHU-Oncogeffe and Labex IGO)...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28779475/molecular-analysis-of-the-expression-of-a-crtb-transgene-and-the-endogenous-psy2-y-1-and-psy2-y-2-genes-of-cassava-and-their-effect-on-root-carotenoid-content
#11
Paul Chavarriaga-Aguirre, Mónica Prías, Danilo López, Darwin Ortiz, Nelson Toro-Perea, Joe Tohme
A conventional breeding program was established to transfer the bacterial phytoene synthase transgene-crtB-from a transgenic, white-rooted cassava to yellow-rooted cassava plants carrying the endogenous phytoene synthase alleles named psy2-y 1 and/or psy2-y 2. Combining endogenous phytoene synthase enzymes (PSYs) with CRTB in a single cassava plant would allow the molecular dissection of individual allele contributions to carotenoid synthesis and/or accumulation in cassava roots. The simultaneous expression of the crtB transgene and psy2-y 2 in individuals planted in the field coincided with higher total, HPLC-quantified carotenoid content in roots, although the variability among replications (plants) precluded the detection of statistically significant differences...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28748301/the-aflatoxin-detoxifizyme-specific-expression-in-the-parotid-gland-of-transgenic-pigs
#12
An-Gang Lou, Jin-Shun Cai, Xue-Mei Zhang, Cheng-Dou Cui, Ying-Shu Piao, Li-Zeng Guan
Producing aflatoxin-detoxifizyme (ADTZ) in pigs to control the AFT contamination of pig feed is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing ADTZ gene in the parotid gland were successfully produced by somatic cell nuclear transfer technology. The ADTZ activity in saliva of 6 transgenic pigs was found to be 7.11 ± 2.63 U/mL. The feeding trial with aflatoxin (AFT) results showed that there were significant difference about the serum biochemical index such as total protein (TP), albumin (ALB), globulin (GLB) contents and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity and AFT residues in serum and liver between the pigs in the test treatment (transgenic pigs) producing ADTZ and those in the positive control (P < 0...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28730514/fostering-responsible-research-with-genome-editing-technologies-a-european-perspective
#13
LETTER
Hervé Chneiweiss, François Hirsch, Lluis Montoliu, Albrecht M Müller, Solveig Fenet, Marion Abecassis, Jennifer Merchant, Bernard Baertschi, Mylène Botbol-Baum, James A Houghton, Mihalis Kritikos, Janet Mifsud, Ewa Bartnik, Johannes Rath, Christiane Druml, Bärbel Friedrich, Ana Sofia Carvalho, Dirk Lanzerath, Agnès Saint-Raymond
In this consensus paper resulting from a meeting that involved representatives from more than 20 European partners, we recommend the foundation of an expert group (European Steering Committee) to assess the potential benefits and draw-backs of genome editing (off-targets, mosaicisms, etc.), and to design risk matrices and scenarios for a responsible use of this promising technology. In addition, this European steering committee will contribute in promoting an open debate on societal aspects prior to a translation into national and international legislation...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28712067/rnai-mediated-resistance-to-whitefly-bemisia-tabaci-in-genetically-engineered-lettuce-lactuca-sativa
#14
Abdulrazak B Ibrahim, Tatiane R Monteiro, Glaucia B Cabral, Francisco J L Aragão
RNA interference (RNAi)-based transgenic technologies have evolved as potent biochemical tools for silencing specific genes of plant pathogens and pests. The approach has been demonstrated to be useful in silencing genes in insect species. Here, we report on the successful construction of RNAi-based plasmid containing an interfering cassette designed to generate dsRNAs that target a novel v-ATPase transcript in whitefly (Bemisia tabaci), an important agricultural pest in tropical and sub-tropical regions. The presence of the transgene was confirmed in T0 and T1 generations of transgenic lettuce lines, segregating in a Mendelian fashion...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28710545/comparative-muscle-proteomics-phosphoproteomics-analysis-provides-new-insight-for-the-biosafety-evaluation-of-fat-1-transgenic-cattle
#15
Xiangbo Xin, Xinfeng Liu, Xin Li, Xiangbin Ding, Shuping Yang, Congfei Jin, Guangpeng Li, Hong Guo
The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO2 enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28681201/gene-editing-tools-state-of-the-art-and-the-road-ahead-for-the-model-and-non-model-fishes
#16
REVIEW
Hirak Kumar Barman, Kiran Dashrath Rasal, Vemulawada Chakrapani, A S Ninawe, Doyil T Vengayil, Syed Asrafuzzaman, Jitendra K Sundaray, Pallipuram Jayasankar
Advancements in the DNA sequencing technologies and computational biology have revolutionized genome/transcriptome sequencing of non-model fishes at an affordable cost. This has led to a paradigm shift with regard to our heightened understandings of structure-functional relationships of genes at a global level, from model animals/fishes to non-model large animals/fishes. Whole genome/transcriptome sequencing technologies were supplemented with the series of discoveries in gene editing tools, which are being used to modify genes at pre-determined positions using programmable nucleases to explore their respective in vivo functions...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28664456/pancreas-specific-expression-of-oncogenes-in-a-porcine-model
#17
Martin F Berthelsen, Morten M Callesen, Tanja S Østergaard, Ying Liu, Rong Li, Henrik Callesen, Frederik Dagnæs-Hansen, Stephen Hamilton-Dutoit, Jannik E Jakobsen, Martin K Thomsen
Pancreatic cancer is the fourth leading course of cancer death and early detection of the disease is crucial for successful treatment. However, pancreatic cancer is difficult to detect in its earliest stages and once symptoms appear, the cancer has often progressed beyond possibility for curing. Research into the disease has been hampered by the lack of good models. We have generated a porcine model of pancreatic cancer with use of transgenic overexpression of an oncogene cassette containing MYC, KRAS (G12D) and SV40 LT...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28646243/provitamin-a-carotenoids-from-an-engineered-high-carotenoid-maize-are-bioavailable-and-zeaxanthin-does-not-compromise-%C3%AE-carotene-absorption-in-poultry
#18
Joana Díaz-Gómez, Jose A Moreno, Eduardo Angulo, Gerhard Sandmann, Changfu Zhu, Teresa Capell, Carmina Nogareda
High-carotenoid (HC) maize, a biofortified staple crop which accumulates β-carotene, β-cryptoxanthin, lutein and zeaxanthin, was used as a feed component in a chicken feeding trial to assess the bioavailability of provitamin A (PVA) carotenoids in the kernel matrix compared to the synthetic and natural color additives routinely used in the poultry industry. We found that the PVA carotenoids in HC maize were not metabolized in the same manner: β-carotene was preferentially converted into retinol in the intestine whereas β-cryptoxanthin accumulated in the liver...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28634819/establishment-of-a-tagged-variant-of-lgr4-receptor-suitable-for-functional-and-expression-studies-in-the-mouse
#19
Vitezslav Kriz, Michaela Krausova, Petra Buresova, Jan Dobes, Dusan Hrckulak, Olga Babosova, Jiri Svec, Vladimir Korinek
Leucine-rich repeat-containing G-protein-coupled receptor 4 (LGR4) is produced in a broad spectrum of mouse embryonic and adult tissues and its deficiency results in embryonal or perinatal lethality. The LGR4 function was mainly related to its potentiation of canonical Wnt signaling; however, several recent studies associate LGR4 with additional signaling pathways. To obtain a suitable tool for studying the signaling properties of Lgr4, we generated a tagged variant of the Lgr4 receptor using gene targeting in the mouse oocyte...
October 2017: Transgenic Research
https://www.readbyqxmd.com/read/28864935/program-and-abstracts-of-the-14th-transgenic-technology-meeting-tt2017-snowbird-resort-salt-lake-city-utah-usa-1-4-october-2017
#20
(no author information available yet)
No abstract text is available yet for this article.
September 2017: Transgenic Research
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