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Transgenic Research

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https://www.readbyqxmd.com/read/28144770/genome-editing-and-the-future-of-farming-meeting-report
#1
Monica Hoyos-Flight, Emilie Brady, Helen Sang, Bruce Whitelaw
No abstract text is available yet for this article.
January 31, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28105543/a-simple-dna-recombination-screening-method-by-rt-pcr-as-an-alternative-to-southern-blot
#2
LETTER
Eliene Albers, Mauro Sbroggiò, Javier Martin-Gonzalez, Alexandra Avram, Stephanie Munk, Andres J Lopez-Contreras
The generation of genetically engineered mouse models (GEMMs), including knock-out (KO) and knock-in (KI) models, often requires genomic screening of many mouse ES cell (mESC) clones by Southern blot. The use of large targeting constructs facilitates the recombination of exogenous DNA in a specific genomic locus, but limits the detection of its correct genomic integration by standard PCR methods. Genomic Long Range PCR (LR-PCR), using primers adjacent to the homology arms, has been used as an alternative to radioactive-based Southern blot screenings...
January 19, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28070852/functional-characterization-of-the-powdery-mildew-susceptibility-gene-smmlo1-in-eggplant-solanum-melongena-l
#3
Valentina Bracuto, Michela Appiano, Luigi Ricciardi, Deniz Göl, Richard G F Visser, Yuling Bai, Stefano Pavan
Eggplant (Solanum melongena L.) is one of the most important vegetables among the Solanaceae and can be a host to fungal species causing powdery mildew (PM) disease. Specific homologs of the plant Mildew Locus O (MLO) gene family are PM susceptibility factors, as their loss of function results in a recessive form of resistance known as mlo resistance. In a previous work, we isolated the eggplant MLO homolog SmMLO1. SmMLO1 is closely related to MLO susceptibility genes characterized in other plant species. However, it displays a peculiar non-synonymous substitution that leads to a T → M amino acid change at protein position 422, in correspondence of the MLO calmodulin-binding domain...
January 9, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28054169/constitutive-expression-of-a-plant-ferredoxin-like-protein-pflp-enhances-capacity-of-photosynthetic-carbon-assimilation-in-rice-oryza-sativa
#4
Hsiang Chang, Hsiang-En Huang, Chin-Fu Cheng, Mei-Hsuan Ho, Mang-Jye Ger
The plant ferredoxin-like protein (PFLP) gene, cloned from sweet peppers predicted as an electron carrier in photosynthesis, shows high homology to the Fd-I sequence of Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Spinacia oleracea. Most of pflp related studies focused on anti-pathogenic effects, while less understanding for the effects in photosynthesis with physiological aspects, such as photosynthesis rate, and levels of carbohydrate metabolites. This project focuses on the effects of pflp overexpression on photosynthesis by physiological evaluations of carbon assimilation with significant higher levels of carbohydrates with higher photosynthesis efficiency...
January 4, 2017: Transgenic Research
https://www.readbyqxmd.com/read/27943082/direct-introduction-of-gene-constructs-into-the-pronucleus-like-structure-of-cloned-embryos-a-new-strategy-for-the-generation-of-genetically-modified-pigs
#5
Mayuko Kurome, Simon Leuchs, Barbara Kessler, Elisabeth Kemter, Eva-Maria Jemiller, Beatrix Foerster, Nikolai Klymiuk, Valeri Zakhartchenko, Eckhard Wolf
Due to a rising demand of porcine models with complex genetic modifications for biomedical research, the approaches for their generation need to be adapted. In this study we describe the direct introduction of a gene construct into the pronucleus (PN)-like structure of cloned embryos as a novel strategy for the generation of genetically modified pigs, termed "nuclear injection". To evaluate the reliability of this new strategy, the developmental ability of embryos in vitro and in vivo as well as the integration and expression efficiency of a transgene carrying green fluorescence protein (GFP) were examined...
December 10, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27905063/efficient-gene-targeting-in-mouse-zygotes-mediated-by-crispr-cas9-protein
#6
Chris J Jung, Junli Zhang, Elizabeth Trenchard, Kent C Lloyd, David B West, Barry Rosen, Pieter J de Jong
The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We characterized several parameters, including single guide RNA cleavage activity and the length and symmetry of homology arms in the construct, and we compared the targeting efficiency between Cas9, Cas9nickase, and dCas9-FokI...
November 30, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27900537/rnai-mediated-resistance-to-rice-black-streaked-dwarf-virus-in-transgenic-rice
#7
Mohamed M S Ahmed, Shiquan Bian, Muyue Wang, Jing Zhao, Bingwei Zhang, Qiaoquan Liu, Changquan Zhang, Shuzhu Tang, Minghong Gu, Hengxiu Yu
Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to RBSDV have not yet been found. One of the most promising methods to confer resistance against RBSDV is the use of RNA interference (RNAi) technology. RBSDV non-structural protein P7-2, encoded by S7-2 gene, is a potential F-box protein and involved in the plant-virus interaction through the ubiquitination pathway...
November 30, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27896535/generation-of-gene-edited-birds-in-one-generation-using-sperm-transfection-assisted-gene-editing-stage
#8
Caitlin A Cooper, Arjun Challagulla, Kristie A Jenkins, Terry G Wise, Terri E O'Neil, Kirsten R Morris, Mark L Tizard, Timothy J Doran
Generating transgenic and gene edited mammals involves in vitro manipulation of oocytes or single cell embryos. Due to the comparative inaccessibility of avian oocytes and single cell embryos, novel protocols have been developed to produce transgenic and gene edited birds. While these protocols are relatively efficient, they involve two generation intervals before reaching complete somatic and germline expressing transgenic or gene edited birds. Most of this work has been done with chickens, and many protocols require in vitro culturing of primordial germ cells (PGCs)...
November 28, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27891561/citrus-psorosis-virus-coat-protein-derived-hairpin-construct-confers-stable-transgenic-resistance-in-citrus-against-psorosis-a-and-b-syndromes
#9
A De Francesco, N Costa, M L García
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation...
November 28, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27888434/a-novel-heavy-metal-atpase-peptide-from-prosopis-juliflora-is-involved-in-metal-uptake-in-yeast-and-tobacco
#10
Nisha S Keeran, G Ganesan, Ajay K Parida
Heavy metal pollution of agricultural soils is one of the most severe ecological problems in the world. Prosopis juliflora, a phreatophytic tree species, grows well in heavy metal laden industrial sites and is known to accumulate heavy metals. Heavy Metal ATPases (HMAs) are ATP driven heavy metal pumps that translocate heavy metals across biological membranes thus helping the plant in heavy metal tolerance and phytoremediation. In the present study we have isolated and characterized a novel 28.9 kDa heavy metal ATPase peptide (PjHMT) from P...
November 25, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27995503/improvement-of-anti-nutritional-effect-resulting-from-%C3%AE-glucanase-specific-expression-in-the-parotid-gland-of-transgenic-pigs
#11
Li-Zeng Guan, Jin-Shun Cai, Shuai Zhao, Yu-Ping Sun, Jing-Lan Wang, Yong Jiang, Gang Shu, Qing-Yan Jiang, Zhen-Fang Wu, Qian-Yun Xi, Yong-Liang Zhang
β-Glucan is the predominant anti-nutritional factors in monogastric animal feed. Although β-glucanase supplementation in diet can help to eliminate the adverse effects, enzyme stability is substantially modified during the feed manufacturing process. To determine whether the expression of endogenous β-glucanase gene (GLU) in vivo can improve digestibility of dietary β-glucan and absorption of nutrients, we successfully produced transgenic pigs via nuclear transfer which express the GLU from Paenibacillus polymyxa CP7 in the parotid gland...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27900536/t%C3%AE-4-overexpression-based-on-the-piggybac-transposon-system-in-cashmere-goats-alters-hair-fiber-characteristics
#12
Bingbo Shi, Qiang Ding, Xiaolin He, Haijing Zhu, Yiyuan Niu, Bei Cai, Jiao Cai, Anming Lei, Danju Kang, Hailong Yan, Baohua Ma, Xiaolong Wang, Lei Qu, Yulin Chen
Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (Tβ4) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (KAP6.1) promoter...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27787698/the-transgenic-expression-of-human-follistatin-344-increases-skeletal-muscle-mass-in-pigs
#13
Fei Chang, Rui Fang, Meng Wang, Xin Zhao, Wen Chang, Zaihu Zhang, Ning Li, Qingyong Meng
Follistatin (FST), which was first found in the follicles of cattle and pigs, has been shown to be an essential regulator for muscle development. Mice that were genetically engineered to overexpress Fst specifically in muscle had at least twice the amount of skeletal muscle mass as controls; these findings are similar to earlier results obtained in myostatin-knockout mice. However, the role of follistatin in skeletal muscle development has yet to be clarified in livestock. Here, we describe transgenic Duroc pigs that exogenously express Fst specifically in muscle tissue...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27778153/phenotypic-characterization-of-a-novel-ho-1-depletion-model-in-the-rat
#14
Vassilios Atsaves, Maria G Detsika, Elpida Poulaki, Hara Gakiopoulou, Elias A Lianos
Although the protective role of HO-1 induction in various forms of kidney disease is well established, mechanisms other than heme catabolism to biliverdin, bilirubin and carbon monoxide have recently been identified. Unraveling these mechanisms requires the generation of appropriate animal models. The present study describes the generation of a HO-1 deficient Hmox1 (-/-) rat model and characterizes its renal and extrarenal phenotype. Hmox1 (-/-) rats had growth retardation and splenomegaly compared to their Hmox1 (+/+) littermates...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27771867/plant-characterization-of-genetically-modified-maize-hybrids-mon-89%C3%A3-34-3%C3%A2-%C3%A3-%C3%A2-mon-88%C3%A3-17-3-mon-89%C3%A3-34-3%C3%A2-%C3%A3-%C3%A2-mon-%C3%A3-%C3%A3-6%C3%A3-3-6-and-mon-%C3%A3-%C3%A3-6%C3%A3-3-6-alternatives-for-maize-production-in-mexico
#15
Oscar Heredia Díaz, José Luis Aldaba Meza, Baltazar M Baltazar, Germán Bojórquez Bojórquez, Luciano Castro Espinoza, José Luis Corrales Madrid, Juan Manuel de la Fuente Martínez, Héctor Abel Durán Pompa, José Alonso Escobedo, Armando Espinoza Banda, José Antonio Garzón Tiznado, Juvencio González García, José Luis Guzmán Rodríguez, Jesús Ignacio Madueño Martínez, José Luis Martínez Carrillo, Chen Meng, Francisco Javier Quiñones Pando, Enrique Rosales Robles, Ignacio Ruiz Hernández, José Elías Treviño Ramírez, Hugo Raúl Uribe Montes, Francisco Zavala García
Environmental risk assessment (ERA) of genetically modified (GM) crops is a process to evaluate whether the biotechnology trait(s) in a GM crop may result in increased pest potential or harm to the environment. In this analysis, two GM insect-resistant (IR) herbicide-tolerant maize hybrids (MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and one herbicide-tolerant GM hybrid (MON-ØØ6Ø3-6) were compared with conventional maize hybrids of similar genetic backgrounds. Two sets of studies, Experimental Phase and Pilot Phase, were conducted across five ecological regions (ecoregions) in Mexico during 2009-2013, and data were subject to meta-analysis...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27744533/zygote-injection-of-crispr-cas9-rna-successfully-modifies-the-target-gene-without-delaying-blastocyst-development-or-altering-the-sex-ratio-in-pigs
#16
Kristin M Whitworth, Joshua A Benne, Lee D Spate, Stephanie L Murphy, Melissa S Samuel, Clifton N Murphy, Jürgen A Richt, Eric Walters, Randall S Prather, Kevin D Wells
The CRISPR/Cas9 genome editing tool has increased the efficiency of creating genetically modified pigs for use as biomedical or agricultural models. The objectives were to determine if DNA editing resulted in a delay in development to the blastocyst stage or in a skewing of the sex ratio. Six DNA templates (gBlocks) that were designed to express guide RNAs that target the transmembrane protease, serine S1, member 2 (TMPRSS2) gene were in vitro transcribed. Pairs of CRISPR guide RNAs that flanked the start codon and polyadenylated Cas9 were co-injected into the cytoplasm of zygotes and cultured in vitro to the blastocyst stage...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27743222/reproductive-parameters-of-double-transgenic-zebrafish-danio-rerio-males-overexpressing-both-the-growth-hormone-gh-and-its-receptor-ghr
#17
Ana Cecilia Gomes Silva, Daniela Volcan Almeida, Bruna Felix Nornberg, Jessica Ribeiro Pereira, Diego Martins Pires, Carine Dahl Corcini, Antonio Sergio Varela Junior, Luis Fernando Marins
Growth hormone (GH) transgenesis presents a high potential application in aquaculture. However, excess GH may have serious consequences due to pleiotropic actions. In order to study these effects in zebrafish (Danio rerio), two transgenic lines were developed. The first expresses GH ubiquitously and constitutively (F0104 line), while the second expresses the GH receptor in a muscle-specific manner (Myo-GHR line). Results from the F0104 line showed accelerated growth but increased reproductive difficulties, while Myo-GHR did not show the expected increase in muscle mass...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27730498/tamoxifen-dosing-for-cre-mediated-recombination-in-experimental-bronchopulmonary-dysplasia
#18
Jordi Ruiz-Camp, José Alberto Rodríguez-Castillo, Susanne Herold, Konstantin Mayer, István Vadász, Michelle D Tallquist, Werner Seeger, Katrin Ahlbrecht, Rory E Morty
Bronchopulmonary dysplasia (BPD) is the most common complication of preterm birth characterized by blunted post-natal lung development. BPD can be modelled in mice by exposure of newborn mouse pups to elevated oxygen levels. Little is known about the mechanisms of perturbed lung development associated with BPD. The advent of transgenic mice, where genetic rearrangements can be induced in particular cell-types at particular time-points during organogenesis, have great potential to explore the pathogenic mechanisms at play during arrested lung development...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27687031/transgenic-expression-of-phytase-in-wheat-endosperm-increases-bioavailability-of-iron-and-zinc-in-grains
#19
Nabeela Abid, Asia Khatoon, Asma Maqbool, Muhammad Irfan, Aftab Bashir, Irsa Asif, Muhammad Shahid, Asma Saeed, Henrik Brinch-Pedersen, Kauser A Malik
Phytate is a major constituent of wheat seeds and chelates metal ions, thus reducing their bioavailability and so the nutritional value of grains. Transgenic plants expressing heterologous phytase are expected to enhance degradation of phytic acid stored in seeds and are proposed to increase the in vitro bioavailability of mineral nutrients. Wheat transgenic plants expressing Aspergillus japonicus phytase gene (phyA) in wheat endosperm were developed till T3 generation. The transgenic lines exhibited 18-99 % increase in phytase activity and 12-76 % reduction of phytic acid content in seeds...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27582300/expression-of-apoplast-targeted-plant-defensin-mtdef4-2-confers-resistance-to-leaf-rust-pathogen-puccinia-triticina-but-does-not-affect-mycorrhizal-symbiosis-in-transgenic-wheat
#20
Jagdeep Kaur, John Fellers, Alok Adholeya, Siva L S Velivelli, Kaoutar El-Mounadi, Natalya Nersesian, Thomas Clemente, Dilip Shah
Rust fungi of the order Pucciniales are destructive pathogens of wheat worldwide. Leaf rust caused by the obligate, biotrophic basidiomycete fungus Puccinia triticina (Pt) is an economically important disease capable of causing up to 50 % yield losses. Historically, resistant wheat cultivars have been used to control leaf rust, but genetic resistance is ephemeral and breaks down with the emergence of new virulent Pt races. There is a need to develop alternative measures for control of leaf rust in wheat. Development of transgenic wheat expressing an antifungal defensin offers a promising approach to complement the endogenous resistance genes within the wheat germplasm for durable resistance to Pt...
February 2017: Transgenic Research
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