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Transgenic Research

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https://www.readbyqxmd.com/read/28916981/expression-of-a-novel-bi-directional-brassica-napus-promoter-in-soybean
#1
Siva Chennareddy, Toby Cicak, Lauren Clark, Sean Russell, Michiyo Skokut, Jeffrey Beringer, Xiaozeng Yang, Yi Jia, Manju Gupta
The expression profile of a natural bi-directional promoter, derived from the Brassica napus EPSPS-A gene, was studied in transgenic soybean (Glycine max C.V. Maverick) lines. Two constructs, pDAB100331 and pDAB100333, were assembled to test the bi-directionality of the promoter. Two reporter genes, gfp and gusA, were employed and they were interchangeably placed in both constructs, one on each end of the promoter such that both proteins expressed divergently in each construct. In the T0 generation, GUS expression was more uniform throughout the leaf of pDAB100333 transgenic plants, where the gusA gene was expressed from the downstream or EPSPS-A end of the bi-directional promoter...
September 15, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28840434/robust-rnai-mediated-resistance-to-infection-of-seven-potyvirids-in-soybean-expressing-an-intron-hairpin-nib-rna
#2
Xiangdong Yang, Lu Niu, Wei Zhang, Hongli He, Jing Yang, Guojie Xing, Dongquan Guo, Qian Du, Xueyan Qian, Yao Yao, Qiyun Li, Yingshan Dong
Viral pathogens, such as soybean mosaic virus (SMV), are a major constraint in soybean production and often cause significant yield loss and quality deterioration. Engineering resistance by RNAi-mediated gene silencing is a powerful strategy for controlling viral diseases. In this study, a 248-bp inverted repeat of the replicase (nuclear inclusion b, NIb) gene was isolated from the SMV SC3 strain, driven by the leaf-specific rbcS2 promoter from Phaseolus vulgaris, and introduced into soybean. The transgenic lines had significantly lower average disease indices (ranging from 2...
August 24, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28819706/generation-of-a-functional-humanized-delta-like-ligand-4-transgenic-mouse-model
#3
John Wiseman, Pernilla Gregersson, Johan Johansson, Kerstin Magnell, Fernanda Pilataxi, Chris Morehouse, Philip Brohawn, Nicholas Holoweckyj, Patrick Strout, Song Cho
Humanized mouse models are important tools in many areas of biological drug development including, within oncology research, the development of antagonistic antibodies that have the potential to block tumor growth by controlling vascularization and are key to the generation of in vivo proof-of-concept efficacy data. However, due to cross reactivity between human antibodies and mouse target such studies regularly require mouse models expressing only the human version of the target molecule. Such humanized knock-in/knock-out, KIKO, models are dependent upon the generation of homozygous mice expressing only the human molecule, compensating for loss of the mouse form...
August 17, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28801873/transgenic-silkworms-secrete-the-recombinant-glycosylated-mrjp1-protein-of-chinese-honeybee-apis-cerana-cerana
#4
Zhengying You, Qiujie Qian, Yiran Wang, Jiaqian Che, Lupeng Ye, Lirong Shen, Boxiong Zhong
Major royal jelly protein-1 (MRJP1) is the most abundant glycoprotein of royal jelly (RJ) and is considered a potential component of functional foods. In this study, we used silkworm transgenic technology to obtain five transgenic silkworm lineages expressing the exogenous recombinant Chinese honeybee, Apis cerana cerana, protein-1 (rAccMRJP1) under the control of a fibroin light chain (Fib-L) promoter in the posterior silk glands. The protein was successfully secreted into cocoons; specifically, the highest rAccMRJP1 protein content was 0...
August 11, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28795260/clock-genes-expression-and-locomotor-activity-are-altered-along-the-light-dark-cycle-in-transgenic-zebrafish-overexpressing-growth-hormone
#5
B P Cruz, L F Brongar, P Popiolek, B S B Gonçalvez, M A Figueiredo, I P G Amaral, V S Da Rosa, L E M Nery, L F Marins
In the present work it was demonstrated that transgenic Danio rerio overexpressing growth hormone (GH-transgenic) present either altered gene expression at a determined time point, or different expression pattern along the LD cycle, when compared with non-transgenic (NT) animals, in the positive and negative loops of the circadian system. Gene expression of clock paralogs was reduced in GH fish at the beginning of the dark phase, leading to diminished expression amplitude along the LD cycle. Furthermore, although no differences were observed between NT and GH animals for bmal1a and cry2b expression at each time point, only GH fish presented amplitude along the LD cycle...
August 9, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28780744/advances-in-transgenic-animal-models-and-techniques
#6
Séverine Ménoret, Laurent Tesson, Séverine Remy, Claire Usal, Laure-Hélène Ouisse, Lucas Brusselle, Vanessa Chenouard, Ignacio Anegon
On May 11th and 12th 2017 was held in Nantes, France, the international meeting "Advances in transgenic animal models and techniques" ( http://www.trm.univ-nantes.fr/ ). This biennial meeting is the fifth one of its kind to be organized by the Transgenic Rats ImmunoPhenomic (TRIP) Nantes facility ( http://www.tgr.nantes.inserm.fr/ ). The meeting was supported by private companies (SONIDEL, Scionics computer innovation, New England Biolabs, MERCK, genOway, Journal Disease Models and Mechanisms) and by public institutions (International Society for Transgenic Technology, University of Nantes, INSERM UMR 1064, SFR François Bonamy, CNRS, Région Pays de la Loire, Biogenouest, TEFOR infrastructure, ITUN, IHU-CESTI and DHU-Oncogeffe and Labex IGO)...
August 5, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28779475/molecular-analysis-of-the-expression-of-a-crtb-transgene-and-the-endogenous-psy2-y-1-and-psy2-y-2-genes-of-cassava-and-their-effect-on-root-carotenoid-content
#7
Paul Chavarriaga-Aguirre, Mónica Prías, Danilo López, Darwin Ortiz, Nelson Toro-Perea, Joe Tohme
A conventional breeding program was established to transfer the bacterial phytoene synthase transgene-crtB-from a transgenic, white-rooted cassava to yellow-rooted cassava plants carrying the endogenous phytoene synthase alleles named psy2-y 1 and/or psy2-y 2. Combining endogenous phytoene synthase enzymes (PSYs) with CRTB in a single cassava plant would allow the molecular dissection of individual allele contributions to carotenoid synthesis and/or accumulation in cassava roots. The simultaneous expression of the crtB transgene and psy2-y 2 in individuals planted in the field coincided with higher total, HPLC-quantified carotenoid content in roots, although the variability among replications (plants) precluded the detection of statistically significant differences...
August 4, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28748301/the-aflatoxin-detoxifizyme-specific-expression-in-the-parotid-gland-of-transgenic-pigs
#8
An-Gang Lou, Jin-Shun Cai, Xue-Mei Zhang, Cheng-Dou Cui, Ying-Shu Piao, Li-Zeng Guan
Producing aflatoxin-detoxifizyme (ADTZ) in pigs to control the AFT contamination of pig feed is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing ADTZ gene in the parotid gland were successfully produced by somatic cell nuclear transfer technology. The ADTZ activity in saliva of 6 transgenic pigs was found to be 7.11 ± 2.63 U/mL. The feeding trial with aflatoxin (AFT) results showed that there were significant difference about the serum biochemical index such as total protein (TP), albumin (ALB), globulin (GLB) contents and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity and AFT residues in serum and liver between the pigs in the test treatment (transgenic pigs) producing ADTZ and those in the positive control (P < 0...
July 26, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28730514/fostering-responsible-research-with-genome-editing-technologies-a-european-perspective
#9
LETTER
Hervé Chneiweiss, François Hirsch, Lluis Montoliu, Albrecht M Müller, Solveig Fenet, Marion Abecassis, Jennifer Merchant, Bernard Baertschi, Mylène Botbol-Baum, James A Houghton, Mihalis Kritikos, Janet Mifsud, Ewa Bartnik, Johannes Rath, Christiane Druml, Bärbel Friedrich, Ana Sofia Carvalho, Dirk Lanzerath, Agnès Saint-Raymond
In this consensus paper resulting from a meeting that involved representatives from more than 20 European partners, we recommend the foundation of an expert group (European Steering Committee) to assess the potential benefits and draw-backs of genome editing (off-targets, mosaicisms, etc.), and to design risk matrices and scenarios for a responsible use of this promising technology. In addition, this European steering committee will contribute in promoting an open debate on societal aspects prior to a translation into national and international legislation...
July 20, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28712067/rnai-mediated-resistance-to-whitefly-bemisia-tabaci-in-genetically-engineered-lettuce-lactuca-sativa
#10
Abdulrazak B Ibrahim, Tatiane R Monteiro, Glaucia B Cabral, Francisco J L Aragão
RNA interference (RNAi)-based transgenic technologies have evolved as potent biochemical tools for silencing specific genes of plant pathogens and pests. The approach has been demonstrated to be useful in silencing genes in insect species. Here, we report on the successful construction of RNAi-based plasmid containing an interfering cassette designed to generate dsRNAs that target a novel v-ATPase transcript in whitefly (Bemisia tabaci), an important agricultural pest in tropical and sub-tropical regions. The presence of the transgene was confirmed in T0 and T1 generations of transgenic lettuce lines, segregating in a Mendelian fashion...
July 15, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28710545/comparative-muscle-proteomics-phosphoproteomics-analysis-provides-new-insight-for-the-biosafety-evaluation-of-fat-1-transgenic-cattle
#11
Xiangbo Xin, Xinfeng Liu, Xin Li, Xiangbin Ding, Shuping Yang, Congfei Jin, Guangpeng Li, Hong Guo
The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO2 enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows...
July 14, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28681201/gene-editing-tools-state-of-the-art-and-the-road-ahead-for-the-model-and-non-model-fishes
#12
REVIEW
Hirak Kumar Barman, Kiran Dashrath Rasal, Vemulawada Chakrapani, A S Ninawe, Doyil T Vengayil, Syed Asrafuzzaman, Jitendra K Sundaray, Pallipuram Jayasankar
Advancements in the DNA sequencing technologies and computational biology have revolutionized genome/transcriptome sequencing of non-model fishes at an affordable cost. This has led to a paradigm shift with regard to our heightened understandings of structure-functional relationships of genes at a global level, from model animals/fishes to non-model large animals/fishes. Whole genome/transcriptome sequencing technologies were supplemented with the series of discoveries in gene editing tools, which are being used to modify genes at pre-determined positions using programmable nucleases to explore their respective in vivo functions...
July 5, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28664456/pancreas-specific-expression-of-oncogenes-in-a-porcine-model
#13
Martin F Berthelsen, Morten M Callesen, Tanja S Østergaard, Ying Liu, Rong Li, Henrik Callesen, Frederik Dagnæs-Hansen, Stephen Hamilton-Dutoit, Jannik E Jakobsen, Martin K Thomsen
Pancreatic cancer is the fourth leading course of cancer death and early detection of the disease is crucial for successful treatment. However, pancreatic cancer is difficult to detect in its earliest stages and once symptoms appear, the cancer has often progressed beyond possibility for curing. Research into the disease has been hampered by the lack of good models. We have generated a porcine model of pancreatic cancer with use of transgenic overexpression of an oncogene cassette containing MYC, KRAS (G12D) and SV40 LT...
June 29, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28864935/program-and-abstracts-of-the-14th-transgenic-technology-meeting-tt2017-snowbird-resort-salt-lake-city-utah-usa-1-4-october-2017
#14
(no author information available yet)
No abstract text is available yet for this article.
September 2017: Transgenic Research
https://www.readbyqxmd.com/read/28631185/the-increased-expression-of-follicle-stimulating-hormone-leads-to-a-decrease-of-fecundity-in-transgenic-large-white-female-pigs
#15
Kai Jiang, Pan Xu, Wanbo Li, Qiang Yang, Longyun Li, Chuanmin Qiao, Huanfa Gong, Hao Zheng, Zhimin Zhou, Hao Fu, Qiuyan Li, Yuyun Xing, Jun Ren
Follicle-stimulating hormone (FSH) is a pituitary gonadotropin regulating reproduction in mammals. Overexpression of the exogenous FSHα/β genes from Chinese Erhualian pigs improved female fecundity of transgenic (TG) mice and male spermatogenesis ability of Large White TG boars. Here, we investigated the impact of the exogenous FSHα/β genes on female reproductive performance of Large White TG pigs. First, we identified the integration site of the exogenous FSHα/β genes at 140,646,456 bp on chromosome 9 in these TG pigs using whole-genome sequencing...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28608322/long-evans-rat-spermatogonial-lines-are-effective-germline-vectors-for-transgenic-rat-production
#16
F Kent Hamra, Christopher T Richie, Brandon K Harvey
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long Evans rat spermatogonial lines were genetically modified by transposon transduction to express a germline, tdTomato reporter gene...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28553699/generation-of-cmahko-gtko-shtnfri-fc-ho-1-quadruple-gene-modified-pigs
#17
Geon A Kim, Eun Mi Lee, Jun-Xue Jin, Sanghoon Lee, Anukul Taweechaipaisankul, Jong Ik Hwang, Zahid Alam, Curie Ahn, Byeong Chun Lee
As an alternative source of organs for transplantation into humans, attention has been directed to pigs due to their similarities in biological features and organ size. However, severe immune rejection has prevented successful xenotransplantation using pig organs and tissues. To overcome immune rejection, recently developed genetic engineering systems such as TALEN coupled with somatic cell nuclear transfer (SCNT) to make embryos could be used to produce pigs compatible with xenotransplantation. We used the TALEN system to target the non-Gal antigen cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene in pigs that is naturally deleted in humans...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28547343/msmir156-affects-global-gene-expression-and-promotes-root-regenerative-capacity-and-nitrogen-fixation-activity-in-alfalfa
#18
Banyar Aung, Ruimin Gao, Margaret Y Gruber, Ze-Chun Yuan, Mark Sumarah, Abdelali Hannoufa
MicroRNA156 (miR156) regulates a network of downstream genes to affect plant growth and development. We previously generated alfalfa (Medicago sativa) plants that overexpress homologous miR156 (MsmiR156OE), and identified three of its SPL target genes. These plants exhibited increased vegetative yield, delayed flowering and longer roots. In this study, we aimed to elucidate the effect of miR156 on the root system, including effect on nodulation and nitrogen fixation. We found that MsmiR156 overexpression increases root regeneration capacity in alfalfa, but with little effect on root biomass at the early stages of root development...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28534229/production-of-a-gonadotropin-releasing-hormone-2-receptor-knockdown-gnrhr2-kd-swine-line
#19
A T Desaulniers, R A Cederberg, G A Mills, C A Lents, B R White
Swine are the only livestock species that produce both the second mammalian isoform of gonadotropin-releasing hormone (GNRH2) and its receptor (GNRHR2). Previously, we reported that GNRH2 and GNRHR2 mediate LH-independent testosterone secretion from porcine testes. To further explore this ligand-receptor complex, a pig model with reduced GNRHR2 expression was developed. Small hairpin RNA sequences targeting porcine GNRHR2 were subcloned into a lentiviral-based vector, lentiviral particles were generated and microinjected into the perivitelline space of zygotes, and embryos were transferred into a recipient...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28526984/reduced-weed-seed-shattering-by-silencing-a-cultivated-rice-gene-strategic-mitigation-for-escaped-transgenes
#20
Huanxin Yan, Lei Li, Ping Liu, Xiaoqi Jiang, Lei Wang, Jia Fang, Zhimin Lin, Feng Wang, Jun Su, Bao-Rong Lu
Transgene flow form a genetically engineered (GE) crop to its wild relatives may result in unwanted environmental consequences. Mitigating transgenes via introducing a gene that is disadvantageous to wild relatives but beneficial to crops, and is tightly-linked with the target transgenes, may provide a promising solution to limit the spread of transgenes in wild/weedy populations. Here we demonstrate a novel system with significantly reduced seed shattering in crop-weed hybrid descendants by partially silenced expression of the seed-shattering gene SH4 in cultivated rice, using artificial microRNA and antisense RNA techniques...
August 2017: Transgenic Research
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