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Plant Cell

Nancy Rosenbaum Hofmann
No abstract text is available yet for this article.
July 18, 2018: Plant Cell
Kiril Mishev, Qing Lu, Bram Denoo, François Peurois, Wim Dejonghe, Jan Hullaert, Riet Maria De Rycke, Sjef Boeren, Marine Bretou, Steven De Munck, Isha Sharma Sharma, Kaija Goodman, Kamila Kalinowska, Véronique Storme, Long Nguyen, Andrzej Drozdzecki, Sara Martins, Wim Nerinckx, Dominique Audenaert, Grégory Vert, Annemieke Madder, Marisa S Otegui, Erika Isono, Savvas Savvides, Wim Annaert, Sacco C de Vries, Jacqueline Cherfils, Johan Winne, Eugenia Russinova
Small GTP-binding proteins from the ADP-ribosylation factor (ARF) family are important regulators of vesicle formation and cellular trafficking in all eukaryotes. ARF activation is accomplished by a protein family of guanine nucleotide exchange factors (GEFs) that contain a conserved catalytic Sec7 domain. Here, we identified and characterized Secdin, a small-molecule inhibitor of Arabidopsis thaliana ARF GEFs. Secdin application caused aberrant retention of plasma membrane (PM) proteins in late endosomal compartments, enhanced vacuolar degradation, impaired protein recycling, and delayed secretion and endocytosis...
July 17, 2018: Plant Cell
Urszula Kania, Tomasz Nodzynski, Qing Lu, Glenn R Hicks, Wim Nerinckx, Kiril Mishev, François Peurois, Jacqueline Cherfils, Riet Maria De Rycke, Peter Grones, Stephanie Robert, Eugenia Russinova, Jiří Friml
The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the polar subcellular localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here, using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin 4 (ES4), an inhibitor of eukaryotic ARF-GEFs...
July 17, 2018: Plant Cell
Lin Zhang, Hua Pu, Zhikun Duan, Yonghong Li, Bei Liu, Qiqi Zhang, Wenjing Li, Jean-David Rochaix, Lin Liu, Lianwei Peng
F-type ATP synthases produce nearly all of the ATP found in cells. The catalytic module, F1, commonly comprises an α3β3 hexamer surrounding a γ/ε stalk. However, it is unclear how these subunits assemble to form a catalytic motor. In this work, we identified and characterized a chloroplast protein that interacts with the CF1β, γ, and ε subunits of the chloroplast ATP synthase and is required for assembly of its F1 module. We have named this protein BFA1 (BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 1) and determined its crystal structure at 2...
July 16, 2018: Plant Cell
Jianhong Hu, Alon Israeli, Naomi Ori, Tai-Ping Sun
Fruit initiation following fertilization in angiosperms is strictly regulated by phytohormones. In tomato (Solanum lycopersicum), auxin and gibberellin (GA) play central roles in promoting fruit initiation. Without fertilization, elevated GA or auxin signaling can induce parthenocarpy (seedless fruit production). The GA-signaling repressor SlDELLA and auxin-signaling components SlIAA9 and SlARF7 repress parthenocarpy, but the underlying mechanism is unknown. Here, we show that SlDELLA and the SlARF7/SlIAA9 complex mediate crosstalk between GA and auxin pathways to regulate fruit initiation...
July 15, 2018: Plant Cell
Fantao Kong, Adrien Burlacot, Yuanxue Liang, Bertrand Legeret, Saleh Alseekh, Yariv Brotman, Alisdair R Fernie, Anja Krieger-Liszkay, Fred Beisson, Gilles Peltier, Yonghua Li-Beisson
Plants and algae must tightly coordinate photosynthetic electron transport and metabolic activities given that they often face fluctuating light and nutrient conditions. The exchange of metabolites and signaling molecules between organelles is thought to be central to this regulation but evidence for this is still fragmentary. Here we show that knocking out the peroxisome-located MALATE DEHYDROGENASE 2 (MDH2) of Chlamydomonas reinhardtii results in dramatic alterations not only in peroxisomal fatty acid breakdown but also in chloroplast starch metabolism and photosynthesis...
July 11, 2018: Plant Cell
Karen-Beth G Scholthof, Sonia Irigoyen, Pilar Catalan, Kranthi Mandadi
The genus Brachypodium represents a model system that is advancing our knowledge of the biology of grasses, including small grains, in the post-genomics era. The most widely used species, Brachypodium distachyon, is a C3 plant that is distributed worldwide. Brachypodium distachyon has a small genome, short lifecycle, and small stature and is amenable to genetic transformation. Due to the intensive and thoughtful development of this grass as a model organism, it is well-suited for laboratory and field experimentation...
July 11, 2018: Plant Cell
Lynn Gl Richardson, Eliana L Small, Hitoshi Inoue, Danny J Schnell
Chloroplast protein import is directed by the interaction of the targeting signal (transit peptide) of nucleus-encoded preproteins with translocons at the outer (TOC) and inner (TIC) chloroplast envelope membranes. Studies of the energetics and determinants of transit peptide binding have led to the hypothesis that import occurs through sequential recognition of transit peptides by components of TOC and TIC to during protein import. To test this hypothesis, we employed a site-specific crosslinking approach to map transit peptide topology in relation to TOC-TIC components at specific stages of import in Arabidopsis thaliana and pea (Pisum sativum)...
July 10, 2018: Plant Cell
Christina Franck, Jens Westermann, Simon Bürssner, Roswitha Lentz, Dmytro Sergiiovych Lituiev, Aurélien Boisson-Dernier
Fast tip-growing plant cells such as pollen tubes (PTs) and root hairs (RHs) require a robust coordination between their internal growth machinery and modifications of their extracellular rigid, yet extensible cell wall (CW). Part of this essential coordination is governed by members of the Catharanthus roseus receptor-like kinase1-like (CrRLK1L) subfamily of RLKs with FERONIA (FER) and its closest homologues, ANXUR1 (ANX1) and ANX2, controlling CW integrity during RH and PT growth, respectively. Recently, four Leucin-Rich-Repeat Extensins 8 to 11 (LRX8-11) were also shown to be important for CW integrity in PTs...
July 10, 2018: Plant Cell
Yi Liao, Xuemei Zhang, Bo Li, Tieyan Liu, Jinfeng Chen, Zetao Bai, Meijiao Wang, Jinfeng Shi, Jason G Walling, Rod A Wing, Jiming Jiang, Mingsheng Chen
Centromeres are dynamic chromosomal regions and the genetic and epigenetic environment of the centromere is often regarded as oppressive to protein-coding genes. Here we used comparative genomic and phylogenomic approaches to study the evolution of centromeres and centromere-linked genes in the genus Oryza. We report a 12.4 Mb high-quality BAC-based pericentromeric assembly for Oryza brachyantha, which diverged from cultivated rice (Oryza sativa) ~15 Mya (million years ago). The synteny analyses reveal seven medium (>50 kb) pericentric inversions in O...
July 2, 2018: Plant Cell
Sandra Irmisch, Seohyun Jo, Christopher R Roach, Sharon Jancsik, Macaire M S Yuen, Lina Madilao, Mark Mark O'Neil-Johnson, Russell Williams, Stephen G Withers, Joerg Bohlmann
Plant specialized metabolism serves as a rich resource of biologically active molecules for drug discovery. The acylated flavonol glycoside montbretin A (MbA) and its precursor mini-MbA are potent inhibitors of human pancreatic α-amylase and are being developed as drug candidates for the treatment of type-2 diabetes. MbA occurs in corms of the ornamental plant montbretia (Crocosmia x crocosmiiflora), but a system or process to obtain large quantities of MbA is not currently available. Improved MbA production thus requires knowledge of its biosynthesis from the flavonol myricetin...
July 2, 2018: Plant Cell
Qiuyue Chen, Yingjia Han, Haijun Liu, Xufeng Wang, Jiamin Sun, Binghao Zhao, Weiya Li, Jinge Tian, Yameng Liang, Jianbing Yan, Xiaohong Yang, Feng Tian
Alternative splicing (AS) enhances transcriptome diversity and plays important roles in regulating plant processes. Although widespread natural variation in AS has been observed in plants, how they are regulated and contribute to phenotypic variation is poorly understood. Here, we report a population-level transcriptome assembly and genome-wide association study to identify splicing quantitative trait loci (sQTLs) in developing maize kernels from 368 inbred lines. We detected 19,554 unique sQTLs for 6,570 genes...
July 2, 2018: Plant Cell
Li Chen, Bing Sun, Wei Gao, Qi-Yang Zhang, Huan Yuan, Min Zhang
Chloroplasts replicate by binary fission, a process driven by ring-like dynamic division machinery at mid-chloroplast. In Arabidopsis thaliana, the first molecular assembly of this machinery, the Z-ring, forms via the association of FtsZ1 and FtsZ2 heteropolymers with the inner envelope membrane through the membrane-tethering protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6 (ARC6). Spatial control of Z-ring assembly ensures the correct placement of the division machinery, and therefore, symmetric chloroplast division...
July 2, 2018: Plant Cell
Minna M Koskela, Annika Brünje, Aiste Ivanauskaite, Magda Grabsztunowicz, Ines Lassowskat, Ulla Neumann, Trinh V Dinh, Julia Sindlinger, Dirk Schwarzer, Markus Wirtz, Esa Tyystjarvi, Iris Finkemeier, Paula Mulo
The amount of light energy received by the photosynthetic reaction centers photosystem II (PSII) and photosystem I (PSI) is balanced through state transitions. Reversible phosphorylation of a light harvesting antenna trimer (L-LHCII) orchestrates the association between L-LHCII and the photosystems thus adjusting the amount of excitation energy received by the reaction centers. In the present study, we identified the enzyme NUCLEAR SHUTTLE INTERACTING (NSI; AT1G32070) as an active lysine acetyltransferase in the chloroplasts of Arabidopsis (Arabidopsis thaliana)...
July 2, 2018: Plant Cell
Robert C Augustine
No abstract text is available yet for this article.
July 2, 2018: Plant Cell
Yasuhiro Higashi, Yozo Okazaki, Kouji Takano, Fumiyoshi Myouga, Kazuo Shinozaki, Eva Knoch, Atsushi Fukushima, Kazuki Saito
Under heat stress, polyunsaturated acyl groups such as α-linolenate (18:3) and hexadecatrienoate (16:3) are removed from chloroplastic glycerolipids in various plant species. Here, we showed that a lipase designated HEAT INDUCIBLE LIPASE1 (HIL1) induces the catabolism of monogalactosyldiacylglycerol (MGDG) under heat stress in Arabidopsis thaliana leaves. Using thermotolerance tests, a T-DNA insertion mutant with disrupted HIL1 was shown to have a heat stress-sensitive phenotype. Lipidomic analysis indicated that the decrease of 34:6-MGDG under heat stress was partially impaired in the hil1 mutant...
July 2, 2018: Plant Cell
Steven M Smith
No abstract text is available yet for this article.
June 22, 2018: Plant Cell
Tina B Schreier, Cléry Antoine, Michael Schläfli, Florian Galbier, Martha Stadler, Emilie Demarsy, Daniele Albertini, Benjamin A Maier, Felix Kessler, Stefan Hörtensteiner, Samuel C Zeeman, Oliver Kötting
Malate dehydrogenases (MDH) convert malate to oxaloacetate using NAD(H) or NADP(H) as a cofactor. Arabidopsis thaliana mutants lacking plastidial NAD-dependent MDH (pdnad-mdh) are embryo-lethal, and constitutive silencing (miR-mdh-1) causes a pale, dwarfed phenotype. The reason for these severe phenotypes is unknown. Here, we rescued the embryo lethality of pdnad-mdh via embryo-specific expression of pdNAD-MDH. Rescued seedlings developed white leaves with aberrant chloroplasts and failed to reproduce. Inducible silencing of pdNAD-MDH at the rosette stage also resulted in white newly emerging leaves...
June 22, 2018: Plant Cell
Ning Zhang, Hong Yu, Hao Yu, Yueyue Cai, Linzhou Huang, Cao Xu, Guosheng Xiong, Xiangbing Meng, Jiyao Wang, Haofeng Chen, Guifu Liu, Yanhui Jing, Yundong Yuan, Yan Liang, Shujia Li, Steven M Smith, Jiayang Li, Yonghong Wang
Tiller angle in cereals is a key shoot architecture trait that strongly influences grain yield. Studies in rice (Oryza sativa L.) have implicated shoot gravitropism in the regulation of tiller angle. However, the functional link between shoot gravitropism and tiller angle is unknown. Here, we conducted a large-scale transcriptome analysis of rice shoots in response to gravistimulation and identified two new nodes of a shoot gravitropism regulatory gene network that also controls rice tiller angle. We demonstrate that HEAT STRESS TRANSCRIPTION FACTOR 2D (HSFA2D) is an upstream positive regulator of the LAZY1-mediated asymmetric auxin distribution pathway...
June 18, 2018: Plant Cell
Eduardo Mateo-Bonmatí, David Esteve-Bruna, Lucía Juan-Vicente, Riad Nadi, Hector Candela, Francisca María Lozano, Maria Rosa Ponce, Jose Manuel Perez-Perez, Jose Luis Micol
All critical developmental and physiological events in a plant's life cycle depend on the proper activation and repression of specific gene sets, and this often involves epigenetic mechanisms. Some Arabidopsis thaliana mutants with disorders of the epigenetic machinery exhibit pleiotropic defects, including incurved leaves and early flowering, due to the ectopic and heterochronic derepression of developmental regulators. Here, we studied one such mutant class, the incurvata11 (icu11) loss-of-function mutants...
June 18, 2018: Plant Cell
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