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Platelets

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https://www.readbyqxmd.com/read/29135309/screening-for-platelet-function-disorders-with-multiplate-and-platelet-function-analyzer
#1
Floor C J I Moenen, Minka J A Vries, Patricia J Nelemans, Katrien J M van Rooy, Jeannique R R A Vranken, Paul W M Verhezen, Rick J H Wetzels, Hugo Ten Cate, Harry C Schouten, Erik A M Beckers, Yvonne M C Henskens
Light transmission aggregation (LTA) is the gold standard for the diagnosis of platelet function disorders (PFDs), but it is time-consuming and limited to specialized laboratories. Whole-blood impedance aggregometry (Multiplate) and platelet function analyzer (PFA) may be used as rapid screening tools to exclude PFDs. The aim of this study is to assess the diagnostic performance of Multiplate and PFA for PFDs, as detected by LTA.Data from preoperative patients, patients referred to the hematologist for bleeding evaluation, and patients with a diagnosed bleeding disorder were used...
November 14, 2017: Platelets
https://www.readbyqxmd.com/read/29125379/bcl-xl-bak-interaction-and-regulation-by-mirna-let-7b-in-the-intrinsic-apoptotic-pathway-of-stored-platelets
#2
Yuzhong Yan, Rufeng Xie, Qi Zhang, Xinfang Zhu, Jia Han, Rong Xia
Bcl-2 family proteins play key roles in the intrinsic apoptosis pathway in platelets, with both pro- and antiapoptotic protein expressions regulating survival during ex vivo storage. We detected a significant decrease in antiapoptotic Bcl-xL and increase in proapoptotic Bak expression on the third day of storage and as a result the ratio of Bak:Bcl-xL also decreased. Moreover, we identified an interaction between Bcl-xL and Bak. These shifts corresponded with activation of the apoptotic pathway, suggesting these proteins might play an important role in platelet survival...
November 10, 2017: Platelets
https://www.readbyqxmd.com/read/29125377/intraflagellar-transport-proteins-are-involved-in-thrombocyte-filopodia-formation-and-secretion
#3
Uvaraj Radhakrishnan, Abdullah Alsrhani, Hemalatha Sundaramoorthi, Gauri Khandekar, Meghana Kashyap, Jannon L Fuchs, Brian D Perkins, Yoshihiro Omori, Pudur Jagadeeswaran
Intraflagellar transport (IFT) proteins are vital for the genesis and maintenance of cilia. Our identification of ift122 transcripts in zebrafish thrombocytes that lack primary cilia was unexpected. IFT proteins serve transport in cilia, whose narrow dimensions may have necessitated the evolution of IFT from vesicular transport in ancestral eukaryotes. We hypothesized that IFTs might also facilitate transport within the filopodia that form when thrombocytes are activated. To test this possibility, we knocked down ift122 expression by injecting antisense Morpholino oligonucleotides (MOs) into zebrafish embryos...
November 10, 2017: Platelets
https://www.readbyqxmd.com/read/29125375/itga2b-and-itgb3-gene-mutations-associated-with-glanzmann-thrombasthenia
#4
Alan T Nurden, Xavier Pillois
No abstract text is available yet for this article.
November 10, 2017: Platelets
https://www.readbyqxmd.com/read/29120698/platelet-microaggregation-in-sepsis-examined-by-quartz-crystal-microbalance-with-dissipation-technology
#5
Caroline M Larkin, Eamon P Breen, Krzysztof A Tomaszewski, Simon Eisele, Marek W Radomski, Thomas A Ryan, Maria-Jose Santos-Martinez
Antiplatelet therapies remain an area of potential interest for the treatment of sepsis; however, studies of platelet aggregation in sepsis have yielded conflicting results. We examined platelet aggregation patterns in patients with septic shock using quartz crystal microbalance with dissipation technology, a microfluidic device capable of measuring platelet microaggregate formation under flow conditions. Platelet aggregation was increased in the washed platelet samples of septic patients. Conversely, these same platelets aggregated less than healthy controls when examined in their plasma...
November 9, 2017: Platelets
https://www.readbyqxmd.com/read/29119855/two-novel-variants-of-uncertain-significance-in-gp9-associated-with-bernard-soulier-syndrome-are-they-true-mutations
#6
P Boisseau, C Debord, M Eveillard, A Quéméner, M Sigaud, M Giraud, P Talarmain, C Thomas, G Landeau, S Bezieau, B Pan Petesch, M C Béné, M Fouassier
Bernard-Soulier syndrome (BSS) is an autosomal recessive major thrombocytopathy, the symptoms of which are mainly marked by mucocutaneous bleeding. This rare disease, initially described in the 1970s, is the result of an abnormal formation of the glycoprotein complex Ib-IX-V (GP Ib-IX-V), a platelet receptor of von Willebrand factor. A large number of mutations, sometimes involving the GP9 gene, have been described as possibly responsible for the disease. We report here the case of a BSS patient who presented with persistent thrombocytopenia (31x10(9)/L) and decreased surface expression of GPIb-IX-V on large platelets with anisocytosis...
November 9, 2017: Platelets
https://www.readbyqxmd.com/read/29119852/platelet-related-indices-in-patients-with-lung-cancer-with-nivolumab
#7
Shosaku Nomura, Takashi Yokoi, Takayasu Kurata
No abstract text is available yet for this article.
November 9, 2017: Platelets
https://www.readbyqxmd.com/read/29119850/dopamine-induces-platelet-production-from-megakaryocytes-via-oxidative-stress-mediated-signaling-pathways
#8
Shilei Chen, Mengjia Hu, Mingqiang Shen, Yang Xu, Cheng Wang, Xinmiao Wang, Fengju Li, Dongfeng Zeng, Fang Chen, Gaomei Zhao, Mo Chen, Fengchao Wang, Tianmin Cheng, Yongping Su, Jinghong Zhao, Song Wang, Junping Wang
Dopamine (DA), a catecholamine neurotransmitter, is known to for its diverse roles on hematopoiesis, yet its function in thrombopoiesis remains poorly understood. This study shows that DA stimulation can directly induce platelet production from megakaryocytes (MKs) in the final stages of thrombopoiesis via a reactive oxygen species (ROS)-dependent pathway. The mechanism was suggested by the results that DA treatment could significantly elevate the ROS levels in MKs, and time-dependently activate oxidative stress-mediated signaling, including p38 mitogen-activated protein kinase, c-Jun NH2-terminal kinase, and caspase-3 signaling pathways, while the antioxidants N-acetylcysteine and L-glutathione could effectively inhibit the activation of these signaling pathways, as well as the ROS increase and platelet production triggered by DA...
November 9, 2017: Platelets
https://www.readbyqxmd.com/read/29090639/platelet-rich-concentrate-in-serum-free-medium-enhances-cartilage-specific-extracellular-matrix-synthesis-and-reduces-chondrocyte-hypertrophy-of-human-mesenchymal-stromal-cells-encapsulated-in-alginate
#9
Shani Samuel, Raja Elina Ahmad, Thamil Selvee Ramasamy, Puvanan Karunanithi, Sangeetha Vasudevaraj Naveen, Tunku Kamarul
Platelet-rich concentrate (PRC), used in conjunction with other chondroinductive growth factors, have been shown to induce chondrogenesis of human mesenchymal stromal cells (hMSC) in pellet culture. However, pellet culture systems promote cell hypertrophy and the presence of other chondroinductive growth factors in the culture media used in previous studies obscures accurate determination of the effect of platelet itself in inducing chondrogenic differentiation. Hence, this study aimed to investigate the effect of PRC alone in enhancing the chondrogenic differentiation potential of human mesenchymal stromal cells (hMSC) encapsulated in three-dimensional alginate constructs...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090621/post-thyroidectomy-hypothyroidism-increases-the-expression-and-activity-of-ectonucleotidases-in-platelets-possible-involvement-of-reactive-oxygen-species
#10
Jucimara Baldissarelli, Micheli M Pillat, Roberta Schmatz, Andréia M Cardoso, Fátima H Abdalla, Juliana S de Oliveira, Carla R N Polachini, Emerson Casali, Clarissa Pereira Bornemann, Henning Ulrich, Vera M Morsch, Maria R C Schetinger
Signaling mediated by purines is a widespread mechanism of cell-cell communication related to vasomotor responses and the control of platelet function in the vascular system. However, little is known about the involvement of this signaling as well as the role of reactive oxygen species (ROS) in the development of hypothyroidism. Therefore, the present study investigates changes in the purinergic system, including enzyme activities and expression in platelets, and oxidative profiles in patients with post-thyroidectomy hypothyroidism...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090618/increased-platelet-reactivity-as-measured-by-plasma-glycoprotein-vi-in-gout
#11
Richard Conway, Claire-Louise Murphy, Anne Madigan, Patricia Kavanagh, Liz Geraghty, Niamh Redmond, Laura Helbert, John J Carey, Eimear Dunne, Dermot Kenny, Geraldine M McCarthy
Patients with gout have an increased risk of cardiovascular events. The glycoprotein VI (GPVI) receptor is found exclusively on platelets and megakaryocytes, is proteolytically cleaved upon platelet activation, and detectable in plasma as soluble GPVI (sGPVI). Therefore, elevated sGPVI is a marker of platelet activation and a risk marker for cardiovascular events. The aim of this study was to assess platelet activation, as measured by plasma sGPVI level in gout. Blood samples were taken from patients with gout or osteoarthritis, and from healthy volunteers...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090616/mouse-podoplanin-supports-adhesion-and-aggregation-of-platelets-under-arterial-shear-a-novel-mechanism-of-haemostasis
#12
Stephanie E Lombard, Alice Y Pollitt, Craig E Hughes, Ying Di, Tom Mckinnon, Chris A O'callaghan, Steve P Watson
The podoplanin-CLEC-2 axis is critical in mice for prevention of hemorrhage in the cerebral vasculature during mid-gestation. This raises the question as to how platelets are captured by podoplanin on neuroepithelial cells in a high shear environment. In this study, we demonstrate that mouse platelets form stable aggregates on mouse podoplanin at arterial shear through a CLEC-2 and Src kinase-dependent pathway. Adhesion and aggregation are also dependent on the platelet glycoprotein (GP) receptors, integrin αIIbβ3 and GPIb, and the feedback agonists ADP and thromboxane A2 (TxA2)...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090612/hermansky-pudlak-syndrome-subtype-5-hps-5-novel-mutation-in-a-65-year-old-with-oculocutaneous-hypopigmentation-and-mild-bleeding-diathesis-the-importance-of-recognizing-a-subtle-phenotype
#13
Juliana Perez Botero, Dong Chen, Julie A Majerus, Lea M Coon, Rong He, Deepti M Warad, Rajiv K Pruthi, William L Nichols
Hermansky-Pudlak syndrome (HPS) - characterized by the distinct clinical phenotypes of both oculocutaneous albinism and mild bleeding diathesis-is caused by mutations in genes that have crucial roles in the assembly of cellular organelles (skin melanosomes, platelet delta [dense] granules, lung lamellar bodies, and cytotoxic T-cell lymphocyte granules). Immunodeficiency, pulmonary fibrosis and granulomatous colitis are associated with some, but not all subtypes of HPS, with varying degrees of clinical severity...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090588/decreased-turnover-aspirin-resistance-by-bidaily-aspirin-intake-and-efficient-cytoreduction-in-myeloproliferative-neoplasms
#14
Andréas Perrier-Cornet, Jean-Christophe Ianotto, Fanny Mingant, Maëla Perrot, Eric Lippert, Hubert Galinat
Essential thrombocythemia (ET) and polycythemia vera (PV) are myeloproliferative neoplasms (MPN) with an increased risk of arterial and venous thrombosis. Aspirin is recommended to reduce this risk, but resistance to antiplatelet therapy seems to hamper its efficacy in some patients. We have previously shown that multiple electrode aggregometry (MEA) was a valuable tool to assess aspirin resistance in MPN. In this study, MEA was used to assess the reduction in aspirin resistance after bi-daily (BID) aspirin intake or cytoreduction...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090587/challenges-on-the-diagnostic-approach-of-inherited-platelet-function-disorders-is-a-paradigm-change-necessary
#15
Tiago Nava, Georges-Etienne Rivard, Arnaud Bonnefoy
Inherited platelet function disorders (IPFD) have been assessed for more than 50 years by aggregation- and secretion-based tests. Several decision trees are available intending to standardize the investigation of IPFD. A large variability of approaches is still in use among the laboratories across the world. In spite of costly and lengthy laboratory evaluation, the results have been found inconclusive or negative in a significant part of patients having bleeding manifestations. Molecular investigation of newly identified IPFD has recently contributed to a better understanding of the complexity of platelet function...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29090586/diagnosis-and-treatment-of-myh9-rd-in-an-australasian-cohort-with-thrombocytopenia
#16
David J Rabbolini, Yenna Chun, Maya Latimer, Shinji Kunishima, Kathleen Fixter, Bhavia Valecha, Peter Tan, Lee Ping Chew, Benjamin T Kile, Rachel Burt, Kottayam Radhakrishnan, Robert Bird, Paul Ockelford, Sara Gabrielli, Qiang Chen, William S Stevenson, Christopher M Ward, Marie-Christine Morel-Kopp
MYH9-related disorders (MYH9-RDs) caused by mutation of the MYH9 gene which encodes non-muscle myosin heavy-chain-IIA (NMMHC-IIA), an important motor protein in hemopoietic cells, are the most commonly encountered cause of inherited macrothrombocytopenia. Despite distinguishing features including an autosomal dominant mode of inheritance, giant platelets on the peripheral blood film accompanied by leucocytes with cytoplasmic inclusion bodies (döhle-like bodies), these disorders remain generally under-recognized and often misdiagnosed as immune thrombocytopenia (ITP)...
November 1, 2017: Platelets
https://www.readbyqxmd.com/read/29072520/abstracts-of-papers-presented-at-the-1-st-italian-uk-platelet-meeting-university-of-bath-7-8-september-2017
#17
Giordano Pula, Ilaria Canobbio, Mauro Torti
No abstract text is available yet for this article.
October 26, 2017: Platelets
https://www.readbyqxmd.com/read/29022492/platelet-aggregation-impacts-thrombin-generation-assessed-by-calibrated-automated-thrombography
#18
Mélusine Didelot, Clémence Docq, Denis Wahl, Patrick Lacolley, Véronique Regnault, Jérémy Lagrange
A calibrated automated thrombogram (CAT) is performed usually with human platelet-free plasma (PFP) but may be more relevant with platelet-rich plasma (PRP). In this case, platelets are not stimulated by subendothelial molecules like collagen. Our aim was to assess the consequence of strong (collagen) or weak (ADP) induction of platelet release and aggregation on thrombin generation. Platelet aggregation in PRP was triggered with 10 µg/mL collagen or 10 µM ADP using a lumi-aggregometer. Thrombin generation curves were monitored by CAT in different conditions: PRP, PRP with activated platelets (actPRP), aggregated PRP (agPRP), aggregated platelets resuspended in autologous PFP (resPRP), PFP and PFP obtained after aggregation (agPFP)...
October 12, 2017: Platelets
https://www.readbyqxmd.com/read/29022423/combination-antiplatelet-treatment-in-coronary-artery-disease-patients-a-necessary-evil-or-an-overzealous-practice
#19
Dimitrios Alexopoulos, Konstantinos Katogiannis, Danai Sfantou, John Lekakis
In seeking to improve care in coronary artery disease patients, further platelet inhibition has been occasionally applied beyond that provided by aspirin and a P2Y12 receptor antagonist. This review aims to offer insights about the rationale, the efficacy and safety of combination antiplatelet therapy, involving three or more agents. Overall, the use of glycoprotein (GP) IIb/IIIa inhibitors did not significantly modify the treatment effect of different antiplatelet strategies, including double vs standard clopidogrel, prasugrel vs clopidogrel, ticagrelor vs clopidogrel, cangrelor vs clopidogrel, and vorapaxar vs placebo...
October 12, 2017: Platelets
https://www.readbyqxmd.com/read/29020490/impact-of-ticagrelor-on-p2y1-and-p2y12-localization-and-on-cholesterol-levels-in-platelet-plasma-membrane
#20
Vahideh Rabani, Damien Montange, Nicolas Meneveau, Siamak Davani
Ticagrelor is an antiplatelet agent that inhibits platelet activation via P2Y12 antagonism. There are several studies showing that P2Y12 needs lipid rafts to be activated, but there are few data about how ticagrelor impacts lipid raft organization. Therefore, we aimed to investigate how ticagrelor could impact the distribution of cholesterol and consequently alter the organization of lipid rafts on platelet plasma membranes. We identified cholesterol-enriched raft fractions in platelet membranes by quantification of their cholesterol levels...
October 11, 2017: Platelets
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