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European Journal of Histochemistry: EJH

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https://www.readbyqxmd.com/read/28735524/characterization-of-cytoskeleton-features-and-maturation-status-of-cultured-human-ipsc-derived-cardiomyocytes
#1
Christian Zuppinger, George Gibbons, Priyanka Dutta-Passecker, Adrian Segiser, Henriette Most, Thomas M Suter
Recent innovations in stem cell technologies and the availability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have opened new possibilities for studies and drug testing on human cardiomyocytes in vitro. Still, there are concerns about the precise nature of such 'reprogrammed' cells. We have performed an investigation using immunocytochemistry and confocal microscopy on several cellular features using commercially available hiPSC-CMs. For some selected developmentally regulated or cardiac chamber-specific proteins, we have compared the results from hiPSC-derived cardiomyocytes with freshly isolated, ventricular cardiomyocytes from adult rats...
June 21, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735523/histochemistry-and-immunolocalisation-of-glucokinin-in-antidiabetic-plants-used-in-traditional-mexican-medicine
#2
Guillermo Laguna-Hernández, Carlos A Rio-Zamorano, Itzel G Meneses-Ochoa, Alicia E Brechú-Franco
Mexico is a megadiverse country that has 3,600 to 4,000 species of medicinal plants, of which approximately 800 are used to treat conditions related to diabetes mellitus (DM). DM is a chronic degenerative disease of energy metabolism that exists as two types: type 1 (DM1) and type 2 (DM2). DM is considered a public health problem that affects 7% of the Mexican population older than 20 years. DM is clinically controlled with hypoglycaemic drugs, alpha-glucosidase inhibitors, insulin secretion stimulants or the direct application of insulin...
June 21, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735527/fibroblast-activating-protein-%C3%AE-expression-in-squamous-cell-carcinoma-of-the-esophagus-in-primary-and-irradiated-tumors-the-use-of-archival-ffpe-material-for-molecular-techniques
#3
Renata Tabola, Magdalena Zaremba-Czogalla, Dagmara Baczynska, Roberto Cirocchi, Kamila Stach, Krzysztof Grabowski, Katarzyna Augoff
There are numerous reports suggesting that fibroblast activating protein-α (FAP-α) plays an important role in invasion of various tumor types. We studied the expression pattern of FAP-α in esophageal squamous cell carcinoma (ESCC) patients who had not been treated primarily and those who had received neoadjuvant radiochemotherapy. Our goal was to establish whether readily available tissue specimens fixed in formalin and stored in paraffin blocks for years might still be a source of FAP-α RNA for PCR analysis...
June 20, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735519/expression-of-the-hpv18-e6-oncoprotein-induces-dna-damage
#4
Elva I Cortés Gutiérrez, Catalina García-Vielma, Adriana Aguilar-Lemarroy, Veronica Vallejo-Ruíz, Patricia Piña-Sánchez, Pablo Zapata-Benavides, Jaime Gosalvez
This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene...
June 15, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735528/lipids-evergreen-autofluorescent-biomarkers-for-the-liver-functional-profiling
#5
Anna Cleta Croce, Giovanni Bottiroli
Depending on their chemical nature, lipids can be classified in two main categories: hydrophilic, greatly contributing to membrane composition and subcellular organelle compartmentalization, and hydrophobic, mostly triglycerides, greatly enrolled in the storage and production of energy. In both cases, some lipid molecules can be involved as signaling agents in the regulation of metabolism and protective or damaging pathways in responses to harmful stimuli. These events could affect in particular the liver, because of its central role in the maintenance of lipid homeostasis...
June 1, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735522/characterization-of-the-role-of-rilp-in-cell-migration
#6
Azzurra Margiotta, Cinzia Progida, Oddmund Bakke, Cecilia Bucci
Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration...
May 31, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735525/oxygen-tension-independent-protection-against-hypoxic-cell-killing-in-rat-liver-by-low-sodium
#7
Andrea Ferrigno, Laura G Di Pasqua, Clarissa Berardo, Veronica Siciliano, Plinio Richelmi, Mariapia Vairetti
The role of Na+ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N2-saturated buffer containing standard (143 mM) or low (25 mM) Na+ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na+, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na+ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining...
May 30, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735520/gemst-a-taylor-made-combination-that-reverts-neuroanatomical-changes-in-stroke
#8
Arturo Mangas, Javier Yajeya, Noelia González, Isabel Ruiz, Marianny Pernìa, Michel Geffard, Rafael Coveñas
In a single transient middle cerebral artery occlusion model of stroke and using immunohistochemical techniques, the effects of a new therapeutic approach named Gemst (a member of the Poly-L-Lysine innovative therapies) have been studied in the rat brain. The expression of inflammatory (CD45, CD11b), oxidative (NO-tryptophan, NO2-tyrosine) and indoleamine 2, 3-dioxygenase pathway (kynurenic acid, 3-hydroxy anthranilic acid) markers has been evaluated in early and late phases of stroke. For this purpose, we have developed eight highly specific monoclonal antibodies directed against some of these markers...
May 25, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735521/myd88-erk-nfkb%C3%A2-pathways-and-pro-inflammatory-cytokines-release-in-periodontal-ligament-stem-cells-stimulated-by-porphyromonas%C3%A2-gingivalis
#9
Francesca Diomede, Maria Zingariello, Marcos F X B Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia De Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani
The present study was aimed at investigating whether human Periodontal Ligament Stem Cells (hPDLSCs) were capable of sensing and reacting to lipopolysaccharide from Porphyromonas gingivalis (LPS-G) which is widely recognized as a major pathogen in the development and progression of periodontitis. At this purpose hPDLCs were stimulated with 5 μg/mL LPS-G various times and the expression of toll-like receptor 4 (TLR4) was evaluated. Toll-like receptors (TLRs) play an essential role in innate immune signaling in response to microbial infections, and in particular TLR4, type-I transmembrane proteins, has been shown recognizing LPS-G...
May 24, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735518/the-possible-prognostic-role-of-histone-deacetylase-and-transforming-growth-factor-%C3%AE-smad-signaling-in-high-grade-gliomas-treated-by-radio-chemotherapy-a-preliminary-immunohistochemical-study
#10
Roberta Sferra, Simona Pompili, Claudio Festuccia, Francesco Marampon, Giovanni L Gravina, Luca Ventura, Ernesto Di Cesare, Sara Cicchinelli, Eugenio Gaudio, Antonella Vetuschi
Glioblastoma (GBM) is the most common and aggressive tumor of the central nervous system. Unfortunately, patients affected by this disease have a very poor prognosis, due to high level of invasiveness and resistance to standard therapies. Although the molecular profile of GBM has been extensively investigated, the events responsible for its pathogenesis and progression remain largely unknown. Histone Deacetylases (HDAC) dependent epigenetic modifications and transforming growth factor (TGF)-β/Smad pathway seem to play an important role in GBM tumorigenesis, resistance to common therapies and poor clinical outcome...
May 16, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735515/determination-of-cell-fate-in-skeletal-muscle-following-bmp-gene-transfer-by-in-vivo-electroporation
#11
Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura
We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood...
May 5, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735526/the-biocompatibility-of-bone-cements-progress-in-methodological-approach
#12
Carlo Dall'Oca, Tommaso Maluta, Gian Mario Micheloni, Matteo Cengarle, Giampaolo Morbioli, Paolo Bernardi, Andrea Sbarbati, Daniele Degl'Innocenti, Franco Lavini, Bruno Magnan
The ideal bone graft substitute should have certain properties and there are many studies dealing with mixture of polymethylmetacrilate (PMMA) and β-tricalciumphospate (β-TCP) presenting the best characteristics of both. Scanning Electron Microscopy (SEM), for ultra-structural data, resulted a very reliable in vivo model to better understand the bioactivity of a cement and to properly evaluate its suitability for a particular purpose. The present study aims to further improve the knowledge on osteointegration development, using both parameters obtained with the Environmental Scanning Electron Microscopy (ESEM) and focused histological examination...
May 4, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735517/metallothionein-expression-and-synthesis-in-the-testis-of-the-lizard-podarcis-sicula-under-natural-conditions-and-following-estrogenic-exposure
#13
Mariailaria Verderame, Ermelinda Limatola, Rosaria Scudiero
Metallothionein (MT) is the main protein involved in the homeostasis of metallic micronutrients and in cellular defence against heavy metals and reactive oxygen species. Found in almost all vertebrate tissues, MT presence and localization in the testis has been controversial. In the present study, by using in situ hybridization and immunohistochemical analysis we assessed the localization of both MT transcript and protein in Podarcis sicula testes during two different phases of the reproductive cycle: the autumnal resumption and the spring-early summer mating period...
May 3, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735516/dnmt1-regulates-il-6-and-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-in-prostate-epithelial-cells
#14
Hui Xu, Yanbo Chen, Qi Chen, Huan Xu, Yanxia Wang, Jiao Yu, Juan Zhou, Zhong Wang, Bing Xu
Multiple factors have been considered to play a role in the development of benign prostatic hyperplasia (BPH), including chronic inflammation, hormones, and epithelial-mesenchymal transition (EMT). Inflammation is regarded as one of the potential inducers of EMT. However, the mechanisms, modulating pro-inflammatory factors (IL-6 and TGF-β1) induce EMT features, have not yet been studied in BPH. In this study, we investigated whether DNA methyltransferases1 (DNMT1) could regulate IL-6 and TGF-β1 induce EMT...
May 3, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348420/reelin-expression-in-human-liver-of-patients-with-chronic-hepatitis-c-infection
#15
Simone Carotti, Giuseppe Perrone, Michelina Amato, Umberto Vespasiani Gentilucci, Daniela Righi, Maria Francesconi, Claudio Pellegrini, Francesca Zalfa, Maria Zingariello, Antonio Picardi, Andrea Onetti Muda, Sergio Morini
Reelin is a secreted extracellular glycoprotein that plays a critical role during brain development. Several studies have described Reelin expression in hepatic stellate cells of the human liver. In order to investigate the possible role of Reelin in the process of hepatic fibrogenesis, in this study we investigated Reelin expression in the liver tissue of patients infected with the Hepatitis C Virus (HCV). On this basis, Reelin expression was analysed by immunohistochemistry during liver biopsies of 81 patients with HCV-related chronic hepatitis...
March 17, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348424/immunohistochemistry-detected-and-localized-cannabinoid-receptor-type-2-in-bovine-fetal-pancreas-at-late-gestation
#16
Cecilia Dall'Aglio, Angela Polisca, Maria Grazia Cappai, Francesca Mercati, Alessandro Troisi, Carolina Pirino, Paola Scocco, Margherita Maranesi
At present, data on the endocannabinoid system expression and distribution in the pancreatic gland appear scarce and controversial as descriptions are limited to humans and laboratory animals. Since the bovine pancreas is very similar to the human in endocrine portion development and control, studies on the fetal gland could prove to be very interesting, as an abnormal maternal condition during late pregnancy may be a predisposing trigger for adult metabolic disorders. The present investigation studied cannabinoid receptor type 2 presence and distribution in the bovine fetal pancreas towards the end of gestation...
March 7, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348422/lectin-like-oxidized-low-density-lipoprotein-receptor-1-deficient-mice-show-resistance-to-age-related-knee-osteoarthritis
#17
Kazuhiko Hashimoto, Yutaka Oda, Fumihisa Nakamura, Ryosuke Kakinoki, Masao Akagi
The lectin-like, oxidized low-density lipoprotein (ox-LDL) receptor-1 (LOX-1)/ox-LDL system contributes to atherosclerosis and may be involved in cartilage degeneration. The purpose of this study was to determine whether the LOX-1/ox-LDL system contributes to age-related osteoarthritis (OA) in vivo, using LOX-1 knockout (LOX-1 KO) mice. Knee cartilage from 6, 12, and 18-month old (n = 10/group) C57Bl/6 wild-type (WT) and LOX-1 KO mice was evaluated by determining the Osteoarthritis Research Society International (OARSI) score of Safranin-O stained samples...
February 14, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348421/the-multifaceted-role-of-podoplanin-expression-in-hepatocellular-carcinoma
#18
Andreea Cioca, Amalia R Ceausu, Irina Marin, Marius Raica, Anca M Cimpean
The role of podoplanin in hepatocellular carcinoma (HCC) is not clear yet. The aim of our study was to evaluate the expression of podoplanin in HCC and to determine its role in hepatocarcinogenesis. We performed immunohistochemistry with monoclonal D2-40 antibody, on paraffin-embedded tissue sections of 72 patients diagnosed with HCC. Lymphatic vessels density (LVD) was increased in patients who had vascular invasion at the time of diagnosis (P=0.018) and in those with associated cirrhosis (P=0.006). Tumor cells showing podoplanin expression were correlated with histological grade (P=0...
February 13, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348423/otx1-and-otx2-as-possible-molecular-markers-of-sinonasal-carcinomas-and-olfactory-neuroblastomas
#19
Cristina Pirrone, Anna M Chiaravalli, Alessandro Marando, Andrea Conti, Alessia Rainero, Andrea Pistochini, Francesco Lo Curto, Francesco Pasquali, Paolo Castelnuovo, Carlo Capella, Giovanni Porta
OTX Homeobox genes are involved in embryonic morphogenesis and in the development of olfactory epithelium in adult. Mutations occurring in the OTX genes are reported to be associated to tumorigenisis in human. No reports correlate the expression of OTX genes and neoplasms of the nasal cavity. Thus, through immunohistochemical and Real-time PCR analysis we investigated OTX1 and OTX2 expression in the more frequent types of nasal and sinonasal tumours. Variable expression of both genes were found in normal sinonasal mucosa and in tumours...
February 9, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28348419/functional-topography-of-the-fully-grown-human-oocyte
#20
Manuela Monti, Alberto Calligaro, Barry Behr, Renee Rejo Pera, Carlo Alberto Redi, Mark Wossidlo
In vivo maturation (IVM) of human oocytes is a technique used to increase the number of usable oocytes for in vitro fertilization (IVF) and represents a necessity for women with different ovarian pathologies. During IVM the oocytes progress from the germinal vesicle stage (GV) through the metaphase II and during this journey both nuclear and cytoplasmic rearrangements must be obtained to increase the probability to get viable and healthy zygotes/embryos after IVF. As the successful clinical outcomes of this technique are a reality, we wanted to investigate the causes behind oocytes maturation arrest...
February 6, 2017: European Journal of Histochemistry: EJH
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