journal
MENU ▼
Read by QxMD icon Read
search

European Journal of Histochemistry: EJH

journal
https://www.readbyqxmd.com/read/28076934/the-injury-of-serotonin-on-intestinal-epithelium-cell-renewal-of-weaned-diarrhoea-mice
#1
Y Dong, C Yang, Z Wang, Z Qin, J Cao, Y Chen
Diarrhoea is a common cause of death in children and weaned animals. Recent research has found that serotonin (5-HT) in the gastrointestinal tract plays an important role in regulating growth and the maintenance of mucosa, which protect against diarrhoea. To determine the influence of 5-HT on intestinal epithelium cell renewal under weaned stress diarrhoea, a weaned-stress diarrhoea mouse model was established with senna infusion (15 mL/Kg) via intragastric administration and stress restraint (SR). Mice with an increase in 5-HT were induced by intraperitoneal injection with citalopram hydrobromide (CH, 10 mg/Kg)...
December 28, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076939/is-there-still-room-for-novelty-in-histochemical-papers
#2
REVIEW
Carlo Pellicciari
Histochemistry continues to be widely applied in biomedical research, being nowadays mostly addressed to detect and locate single molecules or molecular complexes inside cells and tissues, and to relate structural organization and function at the high resolution of the more advanced microscopical techniques. In the attempt to see whether histochemical novelties may be found in the recent literature, the articles published in the European Journal of Histochemistry in the period 2014-2016 have been reviewed. In the majority of the published papers, standardized methods have been preferred by scientists to make their results reliably comparable with the data in the literature, but  many papers (approximately one fourth of the published articles) described novel histochemical methods and procedures...
December 16, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076932/aortic-dissection-is-associated-with-reduced-polycystin-1-expression-an-abnormality-that-leads-to-increased-erk-phosphorylation-in-vascular-smooth-muscle-cells
#3
J Feng, S Ge, L Zhang, H Che, C Liang
The vascular smooth muscle cell (VSMC) phenotypic switch is a key pathophysiological change in various cardiovascular diseases, such as aortic dissection (AD), with a high morbidity. Polycystin-1 (PC1) is significantly downregulated in the VSMCs of AD patients. PC1 is an integral membrane glycoprotein and kinase that regulates different biological processes, including cell proliferation, apoptosis, and cell polarity. However, the role of PC1 in intracellular signaling pathways remains poorly understood. In this study, PC1 downregulation in VSMCs promoted the expression of SM22α, ACTA2 and calponin 1 (CNN1) proteins...
December 16, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076938/transmission-electron-microscopy-for-nanomedicine-novel-applications-for-long-established-techniques
#4
REVIEW
Manuela Malatesta
During the last twenty years, the research in nanoscience and nanotechnology has dramatically increased and, in the last decade, the interest has progressively been oriented towards biomedical applications, giving rise to a new field termed nanomedicine. Transmis - sion electron microscopy is a valuable technique not only for the thorough physico-chemical characterization of newly synthesized nanoparticulates, but especially to explore the effects of nanocomposites on biological systems, providing essential information for the development of efficient therapeutic and diagnostic strategies...
December 9, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076931/varying-effects-of-egf-hgf-and-tgf%C3%AE-on-formation-of-invadopodia-and-invasiveness-of-melanoma-cell-lines-of-different-origin
#5
A Makowiecka, A Simiczyjew, D Nowak, A J Mazur
The understanding of melanoma malignancy mechanisms is essential for patient survival, because melanoma is responsible for ca. 75% of deaths related to skin cancers. Enhanced formation of invadopodia and extracellular matrix (ECM) degradation are two important drivers of cell invasion, and actin dynamics facilitate protrusive activity by providing a driving force to push through the ECM. We focused on the influence of epidermal growth factor (EGF), hepatocyte growth factor (HGF) and transforming growth factor β (TGFβ) on melanoma cell invasiveness, since they are observed in the melanoma microenvironment...
December 9, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076936/histochemical-and-morphological-aspects-of-fresh-frozen-bone-a-preliminary-study
#6
F S De Ponte, G Cutroneo, R Falzea, G Rizzo, L Catalfamo, A Favaloro, G Vermiglio, M Runci, A Centofanti, G Anastasi
Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor...
December 6, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076933/3-hydroxi-anthranilic-acid-is-early-expressed-in-stroke
#7
A Mangas, J Yajeya, N González, I Ruiz, M Geffard, R Coveñas
Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized...
December 2, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076937/in-vivo-imaging-techniques-a-new-era-for-histochemical-analysis
#8
REVIEW
A Busato, P Fumene Feruglio, P P Parnigotto, P Marzola, A Sbarbati
In vivo imaging techniques can be integrated with classical histochemistry to create an actual histochemistry of water. In particular, Magnetic Resonance Imaging (MRI), an imaging technique primarily used as diagnostic tool in clinical/preclinical research, has excellent anatomical resolution, unlimited penetration depth and intrinsic soft tissue contrast. Thanks to the technological development, MRI is not only capable to provide morphological information but also and more interestingly functional, biophysical and molecular...
November 28, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076935/high-temperature-requirement-a1-and-fibronectin-two-possible-players-in-placental-tissue-remodelling
#9
G Tossetta, C Avellini, C Licini, S R Giannubilo, M Castellucci, D Marzioni
High temperature requirement A1 (HtrA1) is a secreted protease involved in placental development. Fibronectin (FN) is involved in important process such as wound healing, cell adhesion and spreading, growth, migration, and differentiation. The purpose of this study was to analyse the expression patterns of HtrA1 in relationship to FN and to the key growth zones of placenta such as mesenchymal villi as well as cell islands and cell columns. We demonstrated that FN and HtrA1 are localized in the placental key growth zones suggesting a pivotal role in maintaining the balance among the molecules involved in the placental development and differentiation...
November 21, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076930/hormone-receptor-expression-in-human-fascial-tissue
#10
C Fede, G Albertin, L Petrelli, M M Sfriso, C Biz, R De Caro, C Stecco
Many epidemiologic, clinical, and experimental findings point to sex differences in myofascial pain in view of the fact that adult women tend to have more myofascial problems with respect to men. It is possible that one of the stimuli to sensitization of fascial nociceptors could come from hormonal factors such as estrogen and relaxin, that are involved in extracellular matrix and collagen remodeling and thus contribute to functions of myofascial tissue. Immunohistochemical and molecular investigations (real-time PCR analysis) of relaxin receptor 1 (RXFP1) and estrogen receptor-alpha (ERα) localization were carried out on sample of human fascia collected from 8 volunteers patients during orthopedic surgery (all females, between 42 and 70 yrs, divided into pre- and post-menopausal groups), and in fibroblasts isolated from deep fascia, to examine both protein and RNA expression levels...
November 2, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076929/non-invasive-imaging-demonstrates-clinical-features-of-ankylosing-spondylitis-in-a-rat-adjuvant-model-a-case-study
#11
N Accart, J Dawson, F Kolbinger, I Kramer, N Beckmann
Ankylosing spondylitis is a common rheumatic disease involving both inflammatory erosive osteopenia and bony overgrowth. Main disease features are recapitulated in small rodents challenged with complete Freund's adjuvant. MRI was used to follow longitudinally in vivo changes induced in the rat spine and micro-CT as terminal assessment of bone damage. Histochemistry methods were used to validate these imaging modalities in view of preclinical drug testing and translational applications of spine imaging. Animals were examined using a 3D fat-suppressed gradient-echo sequence, following the injection of gadolinium...
November 2, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28076928/rxr%C3%AE-is-upregulated-in-first-trimester-endometrial-glands-of-spontaneous-abortions-unlike-lxr-and-ppar%C3%AE
#12
J Knabl, A Vattai, R Hüttenbrenner, S Hutter, M Karsten, U Jeschke
Nuclear receptors are necessary for uterine invasion of the trophoblast and therefore important for maintaining a viable pregnancy. The aim of this study was to investigate the expression pattern and frequency of LXR, PPARγ and RXRα under physiological circumstances and in spontaneous abortions in endometrial glands and decidual tissue cells. A total of 28 (14 physiologic pregnancies/14 spontaneous abortion) human pregnancies in first trimester were analysed for expression of the nuclear receptors LXR, RXRα and PPARγ...
November 2, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734998/lorenzo-gotte-1926-1991-a-pioneer-of-elastin
#13
M Onisto, S Garbisa, M Spina
Lorenzo Gotte (1926-1991) was an outstanding histologist at the School of Medicine of Padua. This year marks the 25th anniversary of his passing away - commemorated during the recent congress of the Italian Society for the Connective Tissue (SISC), held in Padua (September 30 - October 1, 2016). This brief note recalls this outstanding figure: indeed, forthose who knew him, Lorenzo Gotte was an exceptional scientist and at the same time, an unparalleled teacher - and, for many, a great friend. It is still difficult to separate these aspects of his personality, so intertwined in his life: studying elastin and elastic tissue was a passion central to Gotte's life...
September 30, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734992/identification-of-5-hydroxytryptamine-producing-cells-by-detection-of-fluorescence-in-paraffin-embedded-tissue-sections
#14
Y Kaneko, N Onda, Y Watanabe, M Shibutani
5-Hydroxytryptamine (5-HT) produced by enterochromaffin (EC) cells is an important enteric mucosal signaling ligand and has been implicated in several gastrointestinal diseases, including inflammatory bowel disease and functional disorders such as irritable bowel syndrome. The present study reports a new, simple and rapid visualization method of 5-HT-producing EC cells utilizing detection of autofluorescence in paraffin-embedded tissue sections after formalin fixation. In human samples, there was a high incidence of autofluorescence+ cells in the 5-HT+ cells in the pyloric, small intestinal and colonic glands, while co-localization was lacking between autofluorescence+ and gastrin+ cells in the pyloric and small intestinal glands...
September 29, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734990/interindividual-variability-in-the-expression-of-surfactant-protein-a-and-b-in-the-human-lung-during-development
#15
F Cau, E Pisu, C Gerosa, G Senes, F Ronchi, C Botta, E Di Felice, F Uda, V Marinelli, G Faa, V Fanos, C Moretti, D Fanni
The surfactant complex, thanks to its multiple actions including decrease of surface- tension and antimicrobial activity, plays a fundamental role in newborn survival, lowering the risk of respiratory distress syndrome. The aim of this work was to determine if the synthesis of two surfactant proteins (SP), SPA and pro-SPB, shows some inter-individual variability during lung development in the intrauterine life. Immunoreactivity for SPA and pro-SPB was investigated in the lungs of  40 subjects, including 15 fetuses, ranging from 14 to 22 weeks of gestation, and 25 neonates, from 24 to 41 weeks...
September 26, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734994/no-tryptophan-a-new-small-molecule-located-in-the-rat-brain
#16
A Mangas, J Yajeya, N González, S Duleu, M Geffard, R Coveñas
A highly specific monoclonal antibody directed against nitric oxide-tryptophan (NO-W) with good affinity (10-9 M) and specificity was developed. In the rat brain, using an indirect immunoperoxidase technique, cell bodies containing NO-W were exclusively found in the intermediate and dorsal parts of the lateral septal nucleus. No immunoreactive fibres were found in the rat brain. This work reports the first visualization and the morphological characteristics of cell bodies containing NO-W in the mammalian brain...
September 22, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734996/stem-progenitor-cells-in-the-developing-human-cerebellum-an-immunohistochemical-study
#17
V Pibiri, A Ravarino, C Gerosa, M C Pintus, V Fanos, G Faa
The aim of this study was to analyze, by immunohistochemistry, the occurrence of stem/progenitor cells localized in the different niches of the developing human cerebellum. To this end, cerebellar samples were obtained from 3 fetuses and 3 newborns ranging, respectively, from 11 to 24 and from 30 to 38 weeks of gestation. Specimens were 10% formalin-fixed, routinely processed and paraffin-embedded; 3 μm-tick sections were immunostained with anti-SOX2 and PAX6 antibodies. Our study evidenced SOX2 and PAX6 immunoreactivity in precursors cells in all six developing human cerebella...
September 19, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734993/cartilage-canals-in-newborn-dogs-histochemical-and-immunohistochemical-findings
#18
A Di Giancamillo, M E Andreis, P Taini, M C Veronesi, M Di Giancamillo, S C Modina
Cartilage canals (CCs) are microscopic structures involved in secondary ossification centers (SOCs) development. The features of CCs were investigated in the humeral and femoral proximal epiphyses of small-sized newborn dogs (from premature to 28 days after birth) with histochemical and immunohistochemical approaches. Masson's Trichrome revealed a ring-shaped area around CCs, which changes in colour from green (immature collagen) to red (mature collagen) as ossification progresses; perichondrium staining always matched the ring colour...
September 15, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734991/neurochemistry-study-of-spinal-cord-in-non-human-primate-sapajus-spp
#19
K R Torres-da-Silva, A V Da Silva, N O Barioni, G W L Tessarin, J A De Oliveira, E Ervolino, J A C Horta-Junior, C A Casatti
The spinal cord is involved in local, ascending and descending neural pathways. Few studies analyzed the distribution of neuromediators in the laminae of non-human primates along all segments. The present study described the classic neuromediators in the spinal cord of the non-human primate Sapajus spp. through histochemical and immunohistochemical methods. Nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d) method showed neuronal somata in the intermediolateral column (IML), central cervical nucleus (CCN), laminae I, II, III, IV, V, VI, VII, VIII and X, besides dense presence of nerve fibers in laminae II and IX...
September 15, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734997/gamete-and-embryo-fetal-origins-of-adult-diseases
#20
Manuela Monti
By putting together the most advanced evidences supporting the 'gamete and embryo-fetal origins of adult diseases' the two editors, Prof. He-Feng Huang and Prof. Jian-Zhong Sheng (Hangzhou, People's Republic of China) did a great workk.....
August 10, 2016: European Journal of Histochemistry: EJH
journal
journal
31031
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"