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Plant Journal: for Cell and Molecular Biology

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https://www.readbyqxmd.com/read/29779236/arabidopsis-inositol-phosphate-kinases-ipk1-and-itpk1-constitute-a-metabolic-pathway-in-maintaining-phosphate-homeostasis
#1
Hui-Fen Kuo, Yu-Ying Hsu, Wei-Chi Lin, Kai-Yu Chen, Teun Munnik, Charles A Brearley, Tzyy-Jen Chiou
Emerging studies have implicated a close link between inositol phosphate (InsP) metabolism and cellular phosphate (Pi ) homeostasis in eukaryotes; however, whether a common InsP species is deployed as an evolutionarily conserved metabolic messenger to mediate Pi signaling remains unknown. Here, using genetics and InsP profiling combined with Pi starvation response (PSR) analysis in Arabidopsis thaliana, we showed that the kinase activity of inositol pentakisphosphate 2-kinase (IPK1), an enzyme required for phytate (inositol hexakisphosphates; InsP6 ) synthesis, is indispensable for maintaining Pi homeostasis under Pi -replete conditions, and inositol 1,3,4-trisphosphate 5/6-kinase 1 (ITPK1) plays an equivalent role...
May 19, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29779229/codeinone-reductase-isoforms-with-differential-stability-efficiency-and-product-selectivity-in-opium-poppy
#2
Mehran Dastmalchi, Limei Chang, Miguel A Torres, Kenneth K S Ng, Peter J Facchini
Codeinone reductase (COR) catalyzes the reversible NADPH-dependent reduction of codeinone to codeine as the penultimate step of morphine biosynthesis in opium poppy (Papaver somniferum). COR also irreversibly reduces neopinone, which forms by spontaneous isomerization in aqueous solution from codeinone, to neopine. In a parallel pathway involving 3-O-desmethylated analogs, COR converts morphinone to morphine, and neomorphinone to neomorphine. Similar to neopine, neomorphine formation by COR is irreversible...
May 19, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29775508/lfr-is-functionally-associated-with-as2-to-mediate-leaf-development-in-arabidopsis
#3
Xiaowei Lin, Dandan Gu, Hongtao Zhao, Yue Peng, Guofang Zhang, Mengge Li, Zhijuan Wang, Xiutang Wang, Sujuan Cui
Leaves are essential organs for plants. We previously identified a functional gene possibly encoding a component of the SWI/SNF complex named LFR (Leaf and Flower Related) in Arabidopsis thaliana. Loss-of-function mutants of LFR displayed obvious defects in leaf morphogenesis, indicating its vital role in leaf development. Here an allelic null mutant of ASYMMETRIC LEAVES2 (AS2), as2-6, was isolated as an enhancer of lfr-1 in petiole length, vasculature pattern and leaf margin development. The lfr as2 double mutants showed enhanced ectopic expression of BP than each of the single mutant, which is consistent with their synergistic genetic enhancement in multiple BP-dependent development processes...
May 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29775500/endosperm-sugar-accumulation-caused-by-mutation-of-phs8-isa1-leads-to-pre-harvest-sprouting-in-rice
#4
Lin Du, Fan Xu, Jun Fang, Shaopei Gao, Jiuyou Tang, Shuang Fang, Hongru Wang, Hongning Tong, Fengxia Zhang, Jinfang Chu, Guodong Wang, Chengcai Chu
Pre-harvest sprouting (PHS) is an unfavorable trait in cereal crops which could seriously decrease grain yield and quality. Although some PHS-associated quantitative trait loci (QTL) or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map-based cloning revealed that PHS8 encodes a starch debranching enzyme named isoamylase1. Mutation in PHS8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm...
May 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29775494/osa-mir164a-targets-osnac60-and-negatively-regulates-rice-immunity-against-the-blast-fungus-magnaporthe-oryzae
#5
Zhaoyun Wang, Yeqiang Xia, Siyuan Lin, Yanru Wang, Baohuan Guo, Xiaoning Song, Shaochen Ding, Liyu Zheng, Ruiying Feng, Shulin Chen, Yalin Bao, Cong Sheng, Xin Zhang, Jianguo Wu, Dongdong Niu, Hailing Jin, Hongwei Zhao
Exploring the regulatory mechanism played by endogenous rice miRNAs in defense responses against the blast disease is of great significance in both resistant variety breeding and disease control management. We identified rice defense-related miRNAs by comparing rice miRNA expression patterns before and after Magnaporthe oryzae strain Guy11 infection. We discovered that osa-miR164a expression reduced upon Guy11 infection at both early and late stages, which was perfectly associated with the induced expression of its target gene, OsNAC60...
May 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29775492/a-mitogen-activated-protein-kinase-phosphatase-influences-grain-size-and-weight-in-rice
#6
Ran Xu, Haiyue Yu, Junmin Wang, Penggen Duan, Baolan Zhang, Jing Li, Yu Li, Jinsong Xu, Jia Lyu, Na Li, Tuanyao Chai, Yunhai Li
Grain size and weight are directly associated with grain yield in crops. However, the molecular mechanisms that set final grain size and weight remain largely unknown. Here, we characterize two large grain mutants, large grain8-1 (large8-1) and large grain8-2 (large8-2). LARGE8 encodes the mitogen-activated protein kinase phosphatase1 (OsMKP1). Loss of function mutations in OsMKP1 results in large grains, while overexpression of OsMKP1 leads to small grains. OsMKP1 determines grain size by restricting cell proliferation in grain hulls...
May 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29772090/lcm-seq-reveals-the-crucial-role-of-lssoc1-in-heat-promoted-bolting-of-lettuce-lactuca-sativa-l
#7
Zijing Chen, Wensheng Zhao, Danfeng Ge, Yingyan Han, Kang Ning, Chen Luo, Shenglin Wang, Renyi Liu, Xiaolan Zhang, Qian Wang
Lettuce (Lactuca sativa L.) is one of the most economically important vegetables. The floral transition in lettuce is accelerated under high temperatures, which can significantly decrease yields. However, the molecular mechanism underlying the floral tranition in lettuce is poorly known. Using laser capture microdissection coupled with RNA sequencing, we isolated shoot apical meristem cells from the bolting-sensitive lettuce line S39 at four critical stages of development. Subsequently, we screened specifically for the flowering-related gene LsSOC1 during the floral transition through comparative transcriptomic analysis...
May 17, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29771466/arabidopsis-vq18-and-vq26-proteins-interact-with-abi5-transcription-factor-to-negatively-modulate-aba-response-during-seed-germination
#8
Jinjing Pan, Houping Wang, Yanru Hu, Diqiu Yu
Seed germination and early seedling establishment, critical developmental stages in the life cycle of seed plants, are modulated by diverse endogenous hormones and the surrounding environment. Arabidopsis ABSCISIC ACID-INSENSITIVE5 (ABI5) is a central transcription factor of abscisic acid (ABA) signaling that represses those processes. ABI5 is precisely modulated at post-translational level; however, whether it interacts with other crucial transcriptional regulators remains to be investigated. In this study, VQ18 and VQ26, two members of recently-identified VQ family, were found to interact with ABI5 in vitro and in vivo...
May 17, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29770515/structural-variation-and-rates-of-genome-evolution-in-the-grass-family-seen-through-comparison-of-sequences-of-genomes-greatly-differing-in-size
#9
Jan Dvorak, Le Wang, Tingting Zhu, Chad M Jorgensen, Karin R Deal, Xiongtao Dai, Matthew W Dawson, Hans-Georg Müller, Ming-Cheng Luo, Ramesh K Ramasamy, Hamid Dehghani, Yong Q Gu, Bikram S Gill, Assaf Distelfeld, Katrien M Devos, Peng Qi, Frank M You, Patrick J Gulick, Patrick E McGuire
Homology was searched with genes annotated in the Aegilops tauschii pseudomolecules against genes annotated in the pseudomolecules of tetraploid wild emmer wheat, Brachypodium distachyon, sorghum, and rice. Similar searches were initiated with genes annotated in the rice pseudomolecules. Matrices of colinear genes and rearrangements in their order were constructed. Optical Bionano genome maps were constructed and used to validate rearrangements unique to the wild emmer and Ae. tauschii genomes. Most common rearrangements were short paracentric inversions and short intrachromosomal translocations...
May 16, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29770510/scf-sniper-4-controls-the-turnover-of-two-redundant-traf-proteins-in-plant-immunity
#10
Jianhua Huang, Chipan Zhu, Xin Li
In mammals, tumor necrosis factor receptor associated factors (TRAFs) are signaling adaptors that regulate diverse physiological processes, including immunity and stress responses. In Arabidopsis, MUSE13 and MUSE14 are redundant TRAF proteins serving as adaptors in the SCFCRP 1 complex to facilitate the turnover of nucleotide-binding domain and leucine-rich repeats (NLR) immune receptors. Degradation of MUSE13 is inhibited by proteasome inhibitor, suggesting that the MUSE13 stability is controlled by the 26S proteasome...
May 16, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29763523/role-of-basic-pentacysteiene-transcription-factors-in-a-sub-set-of-cytokinin-signaling-responses
#11
Carly M Shanks, Andreas Hecker, Chia-Yi Cheng, Luise Brand, Silvio Collani, Markus Schmid, G Eric Schaller, Dierk Wanke, Klaus Harter, Joseph J Kieber
Cytokinin plays diverse roles in plant growth and development, generally acting by modulating gene transcription in target tissues. The type-B ARR transcription factors have emerged as primary targets of cytokinin signaling and are required for essentially all cytokinin-mediated changes in gene expression. The diversity of cytokinin function is likely imparted by the activity of various transcription factors working with the type-B ARRs to alter specific sets of target genes. One potential set of co-regulators modulating the cytokinin response are the BARLEY B-RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC) family of plant-specific transcription factors...
May 15, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29763520/the-arabidopsis-crrlk1l-protein-kinases-bups1-and-bups2-are-required-for-normal-growth-of-pollen-tubes-in-the-pistil
#12
Lei Zhu, Liang-Cui Chu, Yan Liang, Xue-Qin Zhang, Li-Qun Chen, De Ye
In flowering plants, the interaction of pollen tubes with female tissues is important for the accomplishment of double fertilization. Little is known about the mechanisms that underlie signalling between pollen tubes and female tissues. In this study, two Arabidopsis pollen tube-expressed CrRLK1L protein kinases, Buddha's Paper Seal 1 (BUPS1) and BUPS2, were identified as being required for normal tip growth of pollen tubes in the pistil. They are expressed prolifically in pollen and pollen tubes and are localized on the plasma membrane of the pollen tube tip region...
May 15, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29761864/high-throughput-detection-and-screening-of-plantsmodified-by-geneediting-using-quantitative-real-time-pcr
#13
Cheng Peng, Hua Wang, Xiaoli Xu, Xiaofu Wang, Xiaoyun Chen, Wei Wei, Yongmin Lai, Guoquan Liu, Godwin Ian, Jieqin Li, Ling Zhang, Junfeng Xu
Gene editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been developed to detect gene-edited organisms, these techniques are time- and labour-intensive. Meanwhile, few studies have investigated high-throughput detection and screening strategies for plants modified by gene editing. In this study, we developed a simple, sensitive and high-throughput quantitative real-time(qPCR)-based method. The qPCR-based methodexploits two differently labelled probes that are placed within one amplicon at the gene editing target site to simultaneously detect the wild type and a gene-edited mutant...
May 15, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29752751/intact-arabidopsis-rpb1-functions-in-stem-cell-niches-maintenance-and-cell-cycling-control
#14
Qian-Qian Zhang, Li Ying, Zhao-Ying Fu, Xun-Biao Liu, Kai Yuan, Ying Fang, Yan Liu, Gang Li, Xian-Sheng Zhang, Kang Chong, Lei Ge
Plant meristem activity depends on accurate execution of transcriptional networks required for establishing optimum functioning of stem cell niches. An Arabidopsis mutant card1-1 (constitutive auxin response with DR5:GFP) that encodes a truncated RPB1 (RNA Polymerase II's largest subunit) with shortened C-terminal domain (CTD) was identified. Phosphorylation of the CTD repeats of RPB1 is coupled to transcription in eukaryotes. Here we uncover that the truncated CTD of RPB1 disturbed cell cycling and enlarged the size of shoot and root meristem...
May 12, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29752764/analysis-of-gli-d2-locus-identifies-a-genetic-target-for-simultaneously-improving-the-breadmaking-and-health-related-traits-of-common-wheat
#15
Da Li, Huaibing Jin, Kunpu Zhang, Zhaojun Wang, Faming Wang, Yue Zhao, Naxin Huo, Xin Liu, Yong Q Gu, Daowen Wang, Lingli Dong
Gliadins are a major component of wheat seed proteins. However, the complex homoeologous Gli-2 loci (Gli-A2, -B2 and -D2) encoding the α-gliadins in commercial wheat are still poorly understood. Here we analyzed the Gli-D2 locus of Xiaoyan 81 (Xy81), a winter wheat cultivar. A total of 421.091 kb of Gli-D2 sequence was assembled from sequencing multiple bacterial artificial clones, and 10 α-gliadin genes were annotated. Comparative genomic analysis showed that Xy81 carried only eight of the α-gliadin genes of the D genome donor Ae...
May 11, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29752761/spatial-analysis-of-lipid-metabolites-and-expressed-genes-reveals-tissue-specific-heterogeneity-of-lipid-metabolism-in-high-and-low-oil-brassica-napus-l-seeds
#16
Shaoping Lu, Drew Sturtevant, Mina Aziz, Cheng Jin, Qing Li, Kent D Chapman, Liang Guo
Despite the importance of oilseeds to worldwide human nutrition, and more recently to the production of bio-based diesel fuels, the detailed mechanisms regulating seed oil biosynthesis remain partly understood, especially from a tissue-specific perspective. Here, we investigated the spatial distributions of lipid metabolites and transcripts involved in oil biosynthesis from seeds of two low-erucic acid genotypes of Brassica napus with high and low seed oil content. Integrated results from matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) of lipids in situ, lipidome profiling of extracts from seed tissues, and tissue-specific transcriptome analysis revealed complex spatial distribution patterns of lipids and transcripts...
May 11, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29752755/jasmonic-acid-ethylene-signaling-coordinates-hydroxycinnamic-acid-amides-biosynthesis-through-ora59-transcription-factor
#17
Jinbo Li, Kaixuan Zhang, Yu Meng, Jianping Hu, Mengqi Ding, Jiahui Bian, Mingli Yan, Jianming Han, Meiliang Zhou
Hydroxycinnamic acid amides (HCAAs) are a class of antimicrobial metabolites involved in plant defense against necrotrophic pathogens, including Alternaria brassicicola and Botrytis cinerea. The agmatine coumaroyl transferase (AtACT) is the key enzyme to catalyze the last reaction in the biosynthesis of HCAAs, including p-coumaroylagmatine (CouAgm) and feruloylagmatine in Arabidopsis thaliana. However, it is currently unknown about the regulatory mechanism of the AtACT gene expression. Yeast one-hybrid screening using the AtACT promoter as bait isolated the key positive regulator ORA59 that is involved in jasmonic acid/ethylene (JA/ET) mediated plant defense responses...
May 11, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29752744/distinct-heat-shock-factors-and-chromatin-modifications-mediate-the-organ-autonomous-transcriptional-memory-of-heat-stress
#18
Hsiang-Chin Liu, Jörn Lämke, Siou-Ying Lin, Meng-Ju Hung, Kuan-Ming Liu, Yee-Yung Charng, Isabel Bäurle
Plants can be primed by a stress cue to mount a faster or stronger activation of defense mechanisms upon a subsequent stress. A crucial component of such stress priming is the modified reactivation of genes upon recurring stress; however, the underlying mechanisms are poorly understood. Here, we report that dozens of Arabidopsis thaliana genes display transcriptional memory, i.e. stronger upregulation after a recurring heat stress, that lasts for at least three days. We define a set of transcription factors involved in this memory response and show that the transcriptional memory results in enhanced transcriptional activation within minutes after the onset of a heat stress cue...
May 11, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29750477/apple-fruit-acidity-is-genetically-diversified-by-natural-variations-in-three-hierarchical-epistatic-genes-mdsaur37-mdpp2ch-and-mdalmtii
#19
Dongjie Jia, Fei Shen, Yi Wang, Ting Wu, Xuefeng Xu, Xinzhong Zhang, Zhenhai Han
Many efforts have been made to map quantitative trait loci (QTLs) to facilitate practical marker-assisted selection (MAS) in plants. In the present study, we identified four genome-wide major QTLs responsible for apple fruit acidity by MapQTL and BSA-seq analyses using two independent pedigree-based populations. Candidate genes were screened in major QTL regions, and three functional gene markers, including a non-synonymous A/G single nucleotide polymorphism (SNP) in the coding region of MdPP2CH, a 36-bp insertion in the promoter of MdSAUR37, and a previously reported SNP in MdALMTII, were validated to influence the malate content of apple fruits...
May 11, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29744943/the-floral-homeotic-protein-sepallata3-recognizes-target-dna-sequences-by-shape-readout-involving-a-conserved-arginine-residue-in-the-mads-domain
#20
Sandra Käppel, Rainer Melzer, Florian Rümpler, Christian Gafert, Günter Theißen
SEPALLATA3 of Arabidopsis thaliana is a MADS-domain transcription factor and a key regulator of flower development. MADS-domain proteins bind to sequences termed 'CArG-boxes' (consensus 5'-CC(A/T)6 GG-3'). Since only a fraction of the CArG-boxes in the Arabidopsis genome are bound by SEPALLATA3, more elaborate principles have to be discovered to better understand which features turn CArG-boxes into genuine recognition sites. Here, we investigate to which extent the shape of the DNA is involved in a 'shape readout' that contributes to the binding of SEPALLATA3...
May 9, 2018: Plant Journal: for Cell and Molecular Biology
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