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Plant Journal: for Cell and Molecular Biology

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https://www.readbyqxmd.com/read/28715118/microrna159-targeted-slgamyb-transcription-factors-are-required-for-fruit-set-in-tomato
#1
Eder Marques da Silva, Geraldo Felipe Ferreira E Silva, Débora Brussolo Bidoia, Mariana da Silva Azevedo, Frederico Almeida de Jesus, Lilian Ellen Pino, Lázaro Eustáquio Pereira Peres, Esther Carrera, Isabel López-Díaz, Fabio Tebaldi Silveira Nogueira
The transition from flowering to fruit production, namely fruit set, is crucial to ensure successful sexual plant reproduction. Although studies have described the importance of hormones (i.e., auxin and gibberelins) in controlling fruit set after pollination and fertilization, the role of microRNA-based regulation during ovary development and fruit set is still poorly understood. Here we show that microRNA159/GAMYB1 and -2 pathway (the miR159/GAMYB1/2 module) is crucial for tomato ovule development and fruit set...
July 17, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28715115/membrane-topology-and-identification-of-key-residues-of-eadact-a-plant-mboat-with-unusual-substrate-specificity
#2
Tam N T Tran, Jennifer Shelton, Susan Brown, Timothy P Durrett
Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) catalyzes the transfer of an acetyl group from acetyl-CoA to the sn-3 position of diacylglycerol (DAG) to form 3-acetyl-1,2-diacyl-sn-glycerol (acetyl-TAG). EaDAcT belongs to a small, plant-specific subfamily of the membrane bound O-acyltransferases (MBOAT) that acylate different lipid substrates. Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the same fractions as an endoplasmic reticulum specific marker. By mapping the membrane topology of EaDAcT, we obtained an experimentally determined topology model for a plant MBOAT...
July 17, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28710770/an-arabidopsis-kinase-cascade-influences-auxin-responsive-cell-expansion
#3
Tara A Enders, Elizabeth M Frick, Lucia C Strader
Mitogen-activated protein kinase (MPK) cascades are conserved mechanisms of signal transduction across eukaryotes. Despite the importance of MPK proteins in signaling events, specific roles for many Arabidopsis MPK proteins remain unknown. Multiple studies have suggested roles for MPK signaling in a variety of auxin-related processes. To identify MPK proteins with roles in auxin response, we screened mpk insertional alleles and identified mpk1-1 as a mutant that displays hypersensitivity in auxin-responsive cell expansion assays...
July 15, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28696528/endogenous-sequence-patterns-predispose-the-repair-modes-of-crispr-cas9-induced-dna-double-strand-breaks-in-arabidopsis-thaliana
#4
Giang T H Vu, Hieu X Cao, Friedrich Fauser, Bernd Reiss, Holger Puchta, Ingo Schubert
The possibility to predict the outcome of targeted DNA double strand break (DSB) repair would be desirable for genome editing. Furthermore the consequences of mis-repair of potentially cell-lethal DSBs and the underlying pathways are not yet fully understood. Here we study the CRISPR/Cas9-induced mutation spectra at three selected endogenous loci in Arabidopsis thaliana by deep sequencing of long amplicon libraries. Notably, we found sequence-dependent genomic features impacting the DNA repair outcome. Deletions of 1-<1000 bp size and/or very short insertions, deletions >1 kpb (all due to NHEJ) and deletions combined with insertions between 5->100 bp (caused by an SDSA-like mechanism) occurred most frequently at all three loci...
July 11, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28685881/central-metabolite-and-sterol-profiling-divides-tobacco-male-gametophyte-development-and-pollen-tube-growth-into-eight-metabolic-phases
#5
Alexander Helmut Rotsch, Joachim Kopka, Ivo Feussner, Till Ischebeck
While changes in the transcriptome and proteome of developing pollen have been investigated in tobacco and other species, the metabolic consequences remain rather unclear. Here, a broad range of metabolites were investigated in a close succession of developmental stages. Thirteen stages of tobacco male gametophyte development were collected, ranging from tetrads to pollen tubes. Subsequently, the central metabolome and sterol composition were analyzed by GC-MS, monitoring 77 metabolites and 29 non-identified analytes...
July 7, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28671744/the-autonomous-cell-fate-specification-of-basal-cell-lineage-the-initial-round-of-cell-fate-specification-occurs-at-the-two-celled-proembryo-stage
#6
Liang-Huan Qu, Xuemei Zhou, Xinbo Li, Shi-Sheng Li, Jing Zhao, Peng Zhao, Yuan Liu, Meng-Xiang Sun
In angiosperms, the first zygotic division usually gives rise to two daughter cells with distinct morphologies and developmental fates, which is critical for embryo pattern formation. However, it is still unclear when and how these distinct cell fates are specified and whether the cell specification is related to cytoplasmic localization or polarity. Here, we demonstrated that, when isolated from both maternal tissues and the apical cell, a single basal cell could only develop into a typical suspensor, but never an embryo in vitro...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670834/temporal-and-spatial-transcriptomic-and-microrna-dynamics-of-cam-photosynthesis-in-pineapple
#7
Ching Man Wai, Robert VanBuren, Jisen Zhang, Lixian Huang, Wenjing Miao, Patrick P Edger, Won C Yim, Henry D Priest, Blake C Meyers, Todd Mockler, J Andrew C Smith, John C Cushman, Ray Ming
The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in up to 80% higher water-use efficiency than C3 photosynthesis plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple (Ananas comosus (L.) Merr.). The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670824/a-3d-digital-atlas-of-the-nicotiana-tabacum-root-tip-and-its-use-to-investigate-changes-in-the-root-apical-meristem-induced-by-the-agrobacterium-6b-oncogene
#8
Taras Pasternak, Thomas Haser, Thorsten Falk, Olaf Ronneberger, Klaus Palme, Léon Otten
Using the intrinsic Root Coordinate System (iRoCS) Toolbox, a digital atlas at cellular resolution has been constructed for Nicotiana tabacum roots. Mitotic cells and cells labeled for DNA replication with 5-ethynyl-2'-deoxyuridine (EdU) were mapped. The results demonstrate that iRoCS analysis can be applied to thicker roots than those of Arabidopsis thaliana without histological sectioning. A three-dimensional (3-D) analysis of the root tip showed that tobacco roots undergo several irregular periclinal and tangential divisions...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670772/the-ca-2-cam2-binding-transcription-factor-tga3-elevates-lcd-expression-and-h2-s-production-to-bolster-cr-6-tolerance-in-arabidopsis
#9
Huihui Fang, Zhiqiang Liu, Yanping Long, Yali Liang, Zhuping Jin, Liping Zhang, Danmei Liu, Hua Li, Jixian Zhai, Yanxi Pei
Heavy metals (HMs) contamination on agricultural lands not only reduces crop yield but also causes human health concerns. As a plant gasotransmitter, hydrogen sulfide (H2 S) can trigger various defense responses and help reduce HMs accumulation in plants; however, little is known about the regulatory mechanisms of H2 S signaling. Here, we provided evidence to answer the long-standing question how H2 S production is elevated in plant defense against HMs stress. During Arabidopsis' response to chromium (Cr(6+) ) stress, the transcription of L-cysteine desulfhydrase (LCD), the key enzyme for H2 S production, was enhanced through the calcium (Ca(2+) )/calmodulin2 (CaM2)-mediated pathway...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670755/production-of-low-cs-rice-plants-by-inactivation-of-the-k-transporter-oshak1-with-the-crispr-cas-system
#10
Manuel Nieves-Cordones, Sonia Mohamed, Keitaro Tanoi, Natsuko I Kobayashi, Keiko Takagi, Aurore Vernet, Emmanuel Guiderdoni, Christophe Périn, Hervé Sentenac, Anne-Aliénor Véry
Occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μM), cesium (Cs(+) ) can be taken up by crops and transported to their edible parts. Such a plant capacity to take up Cs(+) from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs(+) content in this crop species, such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side effects...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670741/structural-mutagenic-and-in-silico-studies-of-xyloglucan-fucosylation-in-arabidopsis-thaliana-suggest-a-water-mediated-mechanism
#11
Breeanna R Urbanowicz, Vivek S Bharadwaj, Markus Alahuhta, Maria J Peña, Vladimir V Lunin, Yannick J Bomble, Shuo Wang, Jeong-Yeh Yang, Sami T Tuomivaara, Michael E Himmel, Kelley W Moremen, William S York, Michael F Crowley
The mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely due to the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase-1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2-fucosyl residues to xyloglucan side chains - a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28656705/do-chemical-agents-transported-by-xylem-mass-flow-propagate-variation-potentials
#12
Matthew J Evans, Richard J Morris
Long-distance signalling is important for coordinating plant responses to the environment. Variation potentials are a type of long-distance electrical signal that are generated in plants in response to wounding or flaming. Unlike self-propagating action potentials, variation potentials can be measured beyond regions of dead or chemically treated tissue that block signal generation, suggesting a different mode of propagation. Two alternative propagation mechanisms have been proposed: movement of a chemical agent and a pressure wave through the vasculature...
June 27, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28654223/the-pomegranate-punica-granatum-l-genome-and-the-genomics-of-punicalagin-biosynthesis
#13
Gaihua Qin, Chunyan Xu, Ray Ming, Haibao Tang, Romain Guyot, Elena M Kramer, Yudong Hu, Xingkai Yi, Yongjie Qi, Xiangyang Xu, Zhenghui Gao, Haifa Pan, Jianbo Jian, Yinping Tian, Zhen Yue, Yiliu Xu
Pomegranate (Punica granatum L.) is a perennial fruit crop grown since ancient times that has been planted worldwide and is known for its functional metabolites, particularly punicalagins. We have sequenced and assembled the pomegranate genome with 328 Mb anchored into nine pseudo-chromosomes and annotated 29 229 gene models. A Myrtales lineage-specific whole-genome duplication (WGD) event was detected that occurred in the common ancestor before the divergence of pomegranate and Eucalyptus. Repetitive sequences accounted for 46...
June 27, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28650521/jmj27-an-arabidopsis-h3k9-histone%C3%A2-demethylase-modulates-defense-against-pseudomonas-syringae%C3%A2-and%C3%A2-flowering%C3%A2-time
#14
Aditya Dutta, Pratibha Choudhary, Julie Caruana, Ramesh Raina
Histone methylation is known to dynamically regulate diverse developmental and physiological processes. Histone methyl marks are written by methyltransferases and erased by demethylases, and result in modification of chromatin structure to repress or activate transcription. However, little is known about how histone methylation may regulate defense mechanisms and flowering time in plants. Here we report characterization of JmjC DOMAIN-CONTAINING PROTEIN 27 (JMJ27), an Arabidopsis JHDM2 (JmjC domain-containing histone demethylase 2) family protein, which modulates defense against pathogens and flowering time...
June 26, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28644561/the-redox-sensitive-module-of-cyclophilin-20-3-2-cysteine-peroxiredoxin-and-cysteine-synthase-integrates-sulfur-metabolism-and-oxylipin-signaling-in-the-high-light-acclimation-response
#15
Sara M Müller, Shanshan Wang, Wilena Telman, Michael Liebthal, Helena Schnitzer, Andrea Viehhauser, Carsten Sticht, Carolina Delatorre, Markus Wirtz, Rüdiger Hell, Karl-Josef Dietz
The integration of redox- and reactive oxygen species-dependent signaling and metabolic activities are fundamental to plant acclimation to biotic and abiotic stresses. Previous data suggest the existence of a dynamically interacting module in the chloroplast stroma consisting of cyclophilin 20-3 (Cyp20-3), O-acetylserine(thiol)lyase B (OASTL-B), 2-cysteine peroxiredoxins A/B (2-CysPrx) and serine acetyltransferase 2;1 (SERAT2;1). The functionality of this COPS module is influenced by redox stimuli and oxophytodienoic acid (OPDA) which is the precursor for jasmonic acid...
June 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28640939/global-transcriptome-and-co-expression-network-analyses-reveal-cultivar-specific-molecular-signatures-associated-with-seed-development-and-seed-size-weight-determination-in-chickpea
#16
Rohini Garg, Vikash K Singh, Mohan Singh Rajkumar, Vinay Kumar, Mukesh Jain
Seed development is an intricate process regulated via a complex transcriptional regulatory network. To understand the molecular mechanisms governing seed development and seed size/weight in chickpea, we performed a comprehensive analysis of transcriptome dynamics during seed development in two cultivars with contrasting seed size/weight (small seeded, Himchana 1 and large-seeded, JGK 3). Our analysis identified stage-specific expression for a significant proportion (>13%) of the genes. About one-fourth of total genes exhibited significant differential expression in JGK 3 as compared to Himchana 1...
June 22, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28636143/tetratricopeptide-repeat-protein-pyg7-is-essential-for-photosystem-i-assembly-by-interacting-with-psac-in-arabidopsis
#17
Huixia Yang, Pin Li, Aihong Zhang, Xiaogang Wen, Lixin Zhang, Congming Lu
Although progress has been made in determining the structure and understanding the function of photosystem I (PSI), the PSI assembly process remains poorly understood. PsaC is an essential subunit of PSI and participates in the transfer of electrons to ferredoxin. However, how PsaC is assembled during accumulation of the PSI complex is unknown. In the present study, we showed that Pyg7 localized to the stromal thylakoid and associated with the PSI complex. We also showed that Pyg7 interacted with PsaC. Furthermore, we found that the PSI assembly process was blocked following formation of the PsaAB heterodimer in the pyg7 mutant...
June 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28635135/the-gene-expression-landscape-of-pine-seedling-tissues
#18
Rafael A Cañas, Zhen Li, M Belén Pascual, Vanessa Castro-Rodríguez, Concepción Ávila, Lieven Sterck, Yves Van de Peer, Francisco M Cánovas
Conifers dominate vast regions of the Northern hemisphere. They are the main source of raw materials for timber industry as well as a wide range of biomaterials. Despite their inherent difficulties as experimental models for classical plant biology research, the technological advances in genomics research are enabling fundamental studies on these plants. The use of laser capture microdissection followed by transcriptomic analysis is a powerful tool for unravelling the molecular and functional organization of conifer tissues and specialized cells...
June 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28635129/micrornas-involved-in-auxin-signalling-modulate-male-sterility-under-high-temperature-stress-in-cotton-gossypium-hirsutum
#19
Yuanhao Ding, Yizan Ma, Nian Liu, Jiao Xu, Qin Hu, Yaoyao Li, Yuanlong Wu, Sai Xie, Longfu Zhu, Ling Min, Xianlong Zhang
Male sterility caused by long-term high temperature (HT) stress occurs widely in crops. MicroRNAs (miRNAs), a class of endogenous non-coding small RNAs, play an important role in the plant response to various abiotic stresses. To dissect the working principle of miRNAs in male sterility under HT stress in cotton, a total of 112 known miRNAs, 270 novel miRNAs and 347 target genes were identified from anthers of HT-insensitive (84021) and HT-sensitive (H05) cotton cultivars under normal temperature (NT) and HT conditions through small RNA and degradome sequencing...
June 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28635025/arabidopsis-wrky2-and-wrky34-transcription-factors-interact-with-vq20-protein-to-modulate-pollen-development-and-function
#20
Rihua Lei, Xiaoli Li, Zhenbing Ma, Yan Lv, Yanru Hu, Diqiu Yu
Plant male gametogenesis is tightly regulated and involves complex and precise regulations of transcriptional reprogramming. WRKY transcription factors have been demonstrated to play critical roles in plant development and stress responses. Several members of this family physically interact with VQ motif-containing proteins (VQ proteins) to mediate a plethora of programs in Arabidopsis; however, the involvement of WRKY-VQ complexes in plant male gametogenesis remains largely unknown. In this study, we found that WRKY2 and WKRY34 interact with VQ20 both in vitro and in vivo...
June 21, 2017: Plant Journal: for Cell and Molecular Biology
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