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Plant Journal: for Cell and Molecular Biology

David B Medeiros, Leonardo Perez de Souza, Werner C Antunes, Wagner L Araújo, Danilo M Daloso, Alisdair R Fernie
Sucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that the role of sucrose in this process is primarily energetic. Here we used a combination of stomatal aperture assays coupled with kinetic [U-13 C]-sucrose isotope labelling experiments to confirm that sucrose is degraded during light-induced stomatal opening and to define the fate of the C released from sucrose breakdown. We additionally show that addition of sucrose in the medium did not enhance light-induced stomatal opening...
March 15, 2018: Plant Journal: for Cell and Molecular Biology
Elsa Desnoues, Michel Génard, Bénédicte Quilot-Turion, Valentina Baldazzi
The concentrations of sugars in fruit vary with fruit development, environment, and genotype. In general, there were weak correlations between the variations in sugar concentrations and the activities of enzymes directly related with the synthesis or degradation of sugars. This finding suggests that the relationships between enzyme activities and metabolites are often non-linear and are difficult to assess. To simulate the concentrations of sucrose, glucose, fructose and sorbitol during the development of peach fruits, a kinetic model of sugar metabolism was developed by taking advantage of recent profiling data...
March 15, 2018: Plant Journal: for Cell and Molecular Biology
Min Hu, Shuwei Lv, Wenguang Wu, Yongcai Fu, Fengxia Liu, Bingbing Wang, Weiguo Li, Ping Gu, Hongwei Cai, Chuanqing Sun, Zuofeng Zhu
Plant architecture is a key agronomical factor determining crop yield, which has been a major target of cereal crop domestication. The plant architecture transition from the prostrate tiller of typical African wild rice (O. barthii) to the erect tiller of African cultivated rice (Oryza glaberrima) was a key step during African rice domestication. Here we show that PROG7 (PROSTRATE GROWTH 7), a zinc-finger transcription factor gene on chromosome 7, was required for the prostrate growth of African wild rice. Mutations in the promoter region of prog7 reduced the level of gene expression in the tiller base, leading to erect growth in African cultivated rice...
March 14, 2018: Plant Journal: for Cell and Molecular Biology
Tomonori Shinya, Shigetaka Yasuda, Kiwamu Hyodo, Rena Tani, Yuko Hojo, Yuka Fujiwara, Kei Hiruma, Takuma Ishizaki, Yasunari Fujita, Yusuke Saijo, Ivan Galis
Plant defense against herbivores is modulated by herbivore-associated molecular patterns (HAMPs) from oral secretions (OS) and/or saliva of insects. Furthermore, feeding wounds initiate plant's self-damage responses modulated by danger-associated molecular patterns (DAMP) such as immune defense-promoting plant elicitor peptides (Peps). While temporal and spatial co-existence of both patterns during herbivory implies a possibility of their close interactions, molecular mechanisms remain undetermined. Here, we report that exogenous application of rice (Oryza sativa) peptides (OsPeps) can elicit multiple defense responses in rice cell cultures...
March 7, 2018: Plant Journal: for Cell and Molecular Biology
Paja Sijacic, Marko Bajic, Elizabeth C McKinney, Richard B Meagher, Roger B Deal
Cell differentiation is driven by changes in transcription factor (TF) activity and subsequent alterations in transcription. To study this process, differences in TF binding between cell types can be deduced by probing chromatin accessibility. We used cell type-specific nuclei purification followed by the Assay for Transposase Accessible Chromatin (ATAC-seq) to delineate differences in chromatin accessibility and TF regulatory networks between stem cells of the shoot apical meristem (SAM) and differentiated leaf mesophyll cells in Arabidopsis thaliana...
March 7, 2018: Plant Journal: for Cell and Molecular Biology
Adam M Saffer, Vivian F Irish
Rhamnose is required in Arabidopsis thaliana for synthesizing pectic polysaccharides and glycosylating flavonols. RHAMNOSE BIOSYNTHESIS1 (RHM1) encodes a UDP-l-rhamnose synthase, and rhm1 mutants exhibit many developmental defects, including short root hairs, hyponastic cotyledons, and left-handed helically twisted petals and roots. It has been proposed that the hyponastic cotyledons observed in rhm1 mutants are a consequence of abnormal flavonol glycosylation, while the root hair defect is flavonol-independent...
March 5, 2018: Plant Journal: for Cell and Molecular Biology
Ayaka Ito, Chieko Sugita, Mizuho Ichinose, Yoshinobu Kato, Hiroshi Yamamoto, Toshiharu Shikanai, Mamoru Sugita
Pentatricopeptide repeat (PPR) proteins are known to play important roles in posttranscriptional regulation in plant organelles. However, the function of the majority of PPR proteins remains unknown. To examine their functions, Physcomitrella patens PpPPR_66 knockout (KO) mutants were generated and characterized. KO mosses exhibited a wild type-like growth phenotype but showed aberrant chlorophyll fluorescence due to defects in chloroplast NADH dehydrogenase-like (NDH) activity. Immuno-blot analysis suggested that disruption of PpPPR_66 led to a complete loss of the chloroplast NDH complex...
March 5, 2018: Plant Journal: for Cell and Molecular Biology
Neng-Hui Ye, Feng-Zhu Wang, Lu Shi, Mo-Xian Chen, Yun-Ying Cao, Fu-Yuan Zhu, Yi-Zhen Wu, Li-Juan Xie, Tie-Yuan Liu, Ze-Zhuo Su, Shi Xiao, Hao Zhang, Jianchang Yang, Hai-Yong Gu, Xuan-Xuan Hou, Qi-Juan Hu, Hui-Juan Yi, Chang-Xiang Zhu, Jianhua Zhang, Ying-Gao Liu
Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress. In this study, two rice cultivars, upland (Up221, flooding-intolerant) and lowland (Low88, flooding-tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding-tolerant type (T-type) or a flooding-intolerant type (I-type)...
March 1, 2018: Plant Journal: for Cell and Molecular Biology
Suryatapa Ghosh Jha, Emily R Larson, Jordan Humble, David S Domozych, David S Barrington, Mary L Tierney
The large retromer complex participates in diverse endosomal trafficking pathways and is essential for plant developmental programs including cell polarity, programmed cell death, and shoot gravitropism in Arabidopsis. Here we demonstrate that an evolutionarily conserved VPS26 protein (VPS26C; At1G48550) functions in a complex with VPS35A and VPS29 necessary for root hair growth in Arabidopsis. Bimolecular Fluorescence Complementation showed that VPS26C forms a complex with VPS35A in the presence of VPS29 and this is supported by genetic studies showing that vps29 and vps35a mutants exhibit altered root hair growth...
March 1, 2018: Plant Journal: for Cell and Molecular Biology
Valéria Nagy, André Vidal-Meireles, Anna Podmaniczki, Klára Szentmihályi, Gábor Rákhely, Laura Zsigmond, László Kovács, Szilvia Z Tóth
Sulphur limitation may restrain cell growth and viability. In the green alga, Chlamydomonas reinhardtii, sulphur limitation may induce H2 production lasting for several days, to be exploited as a renewable energy source. Sulphur limitation causes a large number of physiological changes, including the inactivation of photosystem II (PSII), leading to the establishment of hypoxia, essential for the increase in hydrogenase expression and activity. The inactivation of PSII has been long assumed to be caused by the sulphur-limited turnover of its reaction center protein, PsbA...
February 23, 2018: Plant Journal: for Cell and Molecular Biology
Xiaofeng Liu, Kang Ning, Gen Che, Shuangshuang Yan, Lijie Han, Ran Gu, Zheng Li, Yiqun Weng, Xiaolan Zhang
Anther and ovule genesis preconditions crop fertilization and fruit production. However, coordinative regulation of anther and ovule development and underlying molecular pathways remain largely elusive. Here, we found that SPOROCYTELESS (SPL)/NOZZLE (NZZ) expression was nearly abolished in a cucumber mutant with severely defective anther and ovule development. CsSPL was expressed specifically in the developing anthers and ovules. Knockdown of CsSPL reduced male and female fertility with malformed pollen and suppressed ovule development...
February 23, 2018: Plant Journal: for Cell and Molecular Biology
Lee Sweetlove
No abstract text is available yet for this article.
February 23, 2018: Plant Journal: for Cell and Molecular Biology
Mohammad Pourkheirandish, Hiroyuki Kanamori, Jianzhong Wu, Shun Sakuma, Frank R Blattner, Takao Komatsuda
Wild barley forms a two-rowed spike with a brittle rachis, whereas domestic barley has two- or six-rowed spikes with a tough rachis. As does domestic barley, "agriocrithon" forms a six-rowed spike; however the spike is brittle the same as wild barley, which makes the origin of agriocrithon obscure. Haplotype analysis of the Six-rowed spike 1 (vrs1) and the Non-brittle rachis 1 (btr1) and 2 (btr2) genes was conducted to infer the origin of agriocrithon barley. Some agriocrithon barley accessions (eu-agriocrithon) carried Btr1 and Btr2 haplotypes that are not found in any cultivars, implying that they are directly derived from wild barley through a mutation at the vrs1 locus...
February 22, 2018: Plant Journal: for Cell and Molecular Biology
Tzu-Yin Liu, Wen-Chun Chou, Wei-Yuan Chen, Ching-Yi Chu, Chen-Yi Dai, Pei-Yu Wu
Despite a great interest of identification of protein-protein interactions (PPIs) in biological systems, only few attempts have been made for a large-scale PPI screen in planta. Unlike biochemical assays, bimolecular fluorescence complementation (BiFC) allows visualization of the transient and weak PPIs in vivo at the subcellular resolution. However, when the non-fluorescent fragments are highly expressed, the spontaneous and irreversible self-assembly of the split halves can easily generate false positives...
February 16, 2018: Plant Journal: for Cell and Molecular Biology
Ran Li, Daqi Fu, Benzhong Zhu, Yunbo Luo, Hongliang Zhu
With the development of high throughput sequencing, plenty of long non-coding RNAs (lncRNAs) have been discovered to play important roles in diverse biological processes. However, the biological functions of most plant lncRNAs are still unknown. Previously, we had discovered a tomato ripening-related lncRNA, lncRNA1459. Here, we cloned the full-length lncRNA1459, which obtained two transcript isoforms. In addition, lncRNA1459 exhibited specific location in the nucleus. Furthermore, in order to fully identify the function of lncRNA1459 in tomato ripening, loss-function mutants of lncRNA1459 were developed using clusterd regularly interspaced short palindromic repeats (CRISPR)/-associated protein 9 (Cas9)-induced genome editing technology...
February 15, 2018: Plant Journal: for Cell and Molecular Biology
Daniela Ben-Tov, Anat Idan-Molakandov, Anat Hugger, Ilan Ben-Shlush, Markus Günl, Bo Yang, Björn Usadel, Smadar Harpaz-Saad
The production of hydrophilic mucilage along the course of seed coat epidermal cell differentiation is a common adaptation in angiosperms. Previous studies identified COBRA-LIKE 2 (COBL2), a member of the COBRA-LIKE gene family, as a novel component required for crystalline cellulose deposition in seed coat epidermal cells. In recent years, Arabidopsis seed coat epidermal cells (SCEs), also called mucilage secretory cells, have emerged as a powerful model system for the study of plant cell wall components biosynthesis, secretion, assembly and de-muro modification...
February 15, 2018: Plant Journal: for Cell and Molecular Biology
Josephine Herbst, Annabel Girke, Mohammad Reza Hajirezaei, Guy Hanke, Bernhard Grimm
Chlorophyll is synthesized from activated glutamate in the tetrapyrrole biosynthesis pathway through at least 20 different enzymatic reactions. Among these, the MgProto monomethylester (MgProtoME) cyclase catalyzes the formation of a fifth isocyclic ring to tetrapyrroles to form protochlorophyllide. The enzyme consists of two proteins. The CHL27 protein is proposed to be the catalytic component, while LCAA/YCF54 likely acts as a scaffolding factor. In comparison to other reactions of chlorophyll biosynthesis, this enzymatic step lacks clear elucidation and it is hardly understood, how electrons are delivered for the NADPH-dependent cyclization reaction...
February 14, 2018: Plant Journal: for Cell and Molecular Biology
Sousuke Imamura, Yuhta Nomura, Tokiaki Takemura, Imran Pancha, Keiko Taki, Kazuki Toguchi, Yuzuru Tozawa, Kan Tanaka
Chloroplasts are plant organelles that carry out oxygenic photosynthesis. Chloroplast biogenesis depends upon chloroplast ribosomes and their translational activity. However, regulation of chloroplast ribosome biogenesis remains an important unanswered question. In this study, we found that inhibition of target of rapamycin (TOR), a general eukaryotic checkpoint kinase, results in a decline in chloroplast ribosomal RNA (rRNA) transcription in the unicellular red alga, Cyanidioschyzon merolae. Upon TOR inhibition, transcriptomics and other analyses revealed increased expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) gene (CmRSH4b), which encodes a homolog of the guanosine 3'-diphosphate 5'-diphosphate (ppGpp) synthetases that modulate rRNA synthesis in bacteria...
February 14, 2018: Plant Journal: for Cell and Molecular Biology
Inês Carqueijeiro, Thomas Dugé de Bernonville, Arnaud Lanoue, Thu-Thuy Dang, Christiana Teijaro, Christian Paetz, Kevin Billet, Angela Mosquera, Audrey Oudin, Sébastien Besseau, Nicolas Papon, Gaëlle Glévarec, Lucia Atehortùa, Marc Clastre, Nathalie Giglioli-Guivarc'h, Bernd Schneider, Benoit St-Pierre, Rodrigo B Andrade, Sarah E O'Connor, Vincent Courdavault
While the characterization of the biosynthetic pathway of monoterpene indole alkaloids (MIAs) in leaves of Catharanthus roseus is now reaching completion, only two enzymes from the root counterpart dedicated to tabersonine metabolism have been identified to date, namely tabersonine 19-hydroxylase (T19H) and minovincine 19-O-acetyltransferase (MAT). Albeit the recombinant MAT catalyzes MIA acetylation at low efficiency in vitro, we demonstrated that MAT was inactive when expressed in yeast and in planta, suggesting an alternative function for this enzyme...
February 13, 2018: Plant Journal: for Cell and Molecular Biology
Yuan Su, Maoyin Li, Liang Guo, Xuemin Wang
Phosphate (Pi) deficiency in soils is a major limiting factor for plant growth. In response to Pi deprivation, one prominent metabolic adaptation in plants is the decrease in membrane phospholipids that consume approximately one third cellular Pi. The level of two phospholipid-hydrolyzing enzymes, phospholipase Dζ2 (PLDζ2) and nonspecific phospholipase C4 (NPC4), is highly induced in Pi-deprived Arabidopsis. To determine the role of PLDζ2 and NPC4 in plant growth under Pi limitation, Arabidopsis plants deficient in both PLDζ2 and NPC4 (npc4pldζ2) were generated and characterized...
February 13, 2018: Plant Journal: for Cell and Molecular Biology
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