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Plant Journal: for Cell and Molecular Biology

Cristina Ruberti, YaShiuan Lai, Federica Brandizzi
The unfolded protein response (UPR) is an ancient signaling pathway that commits to life-or-death outcomes in response to proteotoxic stress in the endoplasmic reticulum (ER). In plants, the membrane-tethered transcription factor bZIP28 and the ribonuclease-kinase IRE1 along with its splicing target, bZIP60, govern the two cytoprotective UPR signaling pathways known to date. The conserved ER membrane-associated BAX inhibitor1 (BI1) modulates ER stress-induced programmed cell death through yet-unknown mechanisms...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
Alex B Brohammer, Thomas Jy Kono, Nathan M Springer, Suzanne E McGaugh, Candice N Hirsch
Maize is a diverse paleotetraploid species with considerable presence/absence variation and copy number variation. One mechanism through which presence/absence variation can arise is differential fractionation. Fractionation refers to the loss of duplicate gene pairs from one of the maize subgenomes during diploidization. Differential fractionation refers to non-shared gene loss events between individuals following a whole genome duplication event. We investigated the prevalence of presence/absence variation resulting from differential fractionation in the syntenic portion of the genome using two whole genome de novo assemblies of the inbred lines B73 and PH207...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
Zhenbing Zheng, Shan Gao, Li Huan, Guang-Ce Wang
Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
Lenka Havlickova, Zhesi He, Lihong Wang, Swen Langer, Andrea L Harper, Harjeevan Kaur, Martin R Broadley, Vasilis Gegas, Ian Bancroft
An updated platform was developed to underpin association genetics studies in the polyploid crop species Brassica napus. Based on 1.92 x 10(12) bases of leaf mRNAseq data, functional genotypes, comprising 355,536 single nucleotide polymorphism markers and transcript abundance were scored across a 383-accession genetic diversity panel using a transcriptome reference comprising 116,098 ordered CDS gene models. The use of the platform for Associative Transcriptomics was first tested by analysing the genetic architecture of variation for seed erucic acid content, as high erucic rapeseed oil is highly valued for a variety of applications in industry...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
Xiaojing Li, Juanying Ye, Hong Ma, Pingli Lu
Protein lysine acetylation (KAC) is a dynamic and reversible post-translational modification, playing important biological roles in many organisms. Although KAC was shown to affect reproductive development and meiosis in yeast and animals, similar studies are largely lacking in flowering plants, especially in a proteome-scale investigation for a certain reproductive stage. Here, we reported results from a proteomic investigation to detect KAC status of the developing rice anthers near the time of meiosis (RAM), providing strong biochemical evidence for roles of many KAC-affected proteins during rice anther development and meiosis...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
Purva Kulkarni, Mina Dost, Özgül Demir Bulut, Alexander Welle, Sebastian Böcker, Wilhelm Boland, Aleš Svatoš
Spatially resolved analysis of a multitude of compound classes has become feasible with the rapid advancement in mass spectrometry imaging strategies. In this study, we present a protocol that combines high lateral resolution TOF-SIMS imaging with a multivariate data analysis (MVA) approach to probe the complex leaf surface chemistry of Populus trichocarpa. Here, epicuticular waxes (EWs) found on the adaxial leaf surface of Populus trichocarpa were blotted on silicon wafers and imaged using time-of-flight secondary ion mass spectrometry (TOF-SIMS) at 10 μm and 1 μm lateral resolution...
November 8, 2017: Plant Journal: for Cell and Molecular Biology
Juan de Dios Barajas-Lopez, Jose Ramon Moreno, Francisco M Gamez-Arjona, Jose M Pardo, Matleena Punkkinen, Jian-Kang Zhu, Francisco J Quintero, Hiroaki Fujii
Sucrose-Non-Fermenting1-related protein kinases (SnRKs) are important for plant growth and stress responses. This family has three clades: SnRK1, SnRK2, and SnRK3. Although plant SnRKs are thought to be activated by upstream kinases, the overall mechanism remains obscure. Geminivirus Rep-Interacting Kinase (GRIK)1 and GRIK2 phosphorylate SnRK1s, which are involved in sugar/energy sensing, and the grik1-1 grik2-1 double mutant shows growth retardation under regular growth conditions. In this study, we established another Arabidopsis mutant line harbouring a different allele of gene GRIK1 (grik1-2 grik2-1) that grows similarly to the wild type, enabling us to evaluate the function of GRIKs under stress conditions...
November 1, 2017: Plant Journal: for Cell and Molecular Biology
Hyunjoo Eom, Su Jung Park, Min Kyung Kim, Hoyeun Kim, Hunseung Kang, Ilha Lee
TATA-binding protein-associated factors (TAFs) are general transcription factors within the TFIID complex, which recognizes the core promoter of genes. In addition to their biochemical function, it is known that several TAFs are involved in the regulation of developmental processes. In this study, we found that TAF15b affects flowering time, especially through the autonomous pathway (AP) in Arabidopsis. The mutant taf15b shows late flowering compared to the wild type during both long and short days, and vernalization accelerates the flowering time of taf15b...
October 31, 2017: Plant Journal: for Cell and Molecular Biology
Ping Su, Hongyu Guan, Yujun Zhao, Yuru Tong, Meimei Xu, Yifeng Zhang, Tianyuan Hu, Jian Yang, Qiqing Cheng, Linhui Gao, Yujia Liu, Jiawei Zhou, Reuben J Peters, Luqi Huang, Wei Gao
Tripterygium wilfordii has long been used as a medicinal plant, and exhibits impressive and effective anti-inflammatory, immunosuppressive and anti-tumor activities. The main active ingredients are diterpenoids and triterpenoids, such as triptolide and celastrol, respectively. A major challenge to harnessing these natural products is that they are found in very low amounts in planta. Access has been further limited by the lack of knowledge regarding the underlying biosynthetic pathways, particularly for the abeo-abietane tri-epoxide lactone triptolide...
October 31, 2017: Plant Journal: for Cell and Molecular Biology
Hiroko Yamaya-Ito, Yoshikazu Shimoda, Tsuneo Hakoyama, Shusei Sato, Takakazu Kaneko, Md Shakhawat Hossain, Satoshi Shibata, Masayoshi Kawaguchi, Makoto Hayashi, Hiroshi Kouchi, Yosuke Umehara
The nitrogen-fixing symbiosis of legumes and Rhizobium bacteria is established by complex interactions between the two symbiotic partners. Legume Fix(-) mutants form apparently normal nodules with endosymbiotic rhizobia but fail to induce rhizobial nitrogen fixation. These mutants are useful for identifying the legume genes involved in the interactions essential for symbiotic nitrogen fixation. We describe here a Fix(-) mutant of Lotus japonicus, apn1, which showed a very specific symbiotic phenotype. It formed ineffective nodules when inoculated with the Mesorhizobium loti strain TONO...
October 31, 2017: Plant Journal: for Cell and Molecular Biology
Junbo Gou, Fuhua Hao, Chongyang Huang, Moonhyuk Kwon, Fangfang Chen, Changfu Li, Chaoyang Liu, Dae-Kyun Ro, Huiru Tang, Yansheng Zhang
Sesquiterpene lactones (STLs) are C15 terpenoid natural products with α-methylene γ-lactone moiety. A large proportion of STLs in Asteraceae species is derived from the central precursor germacrene A acid (GAA). Formation of the lactone rings depends on the regio- (C6 or C8) and stereo-selective (α- or β-) hydroxylations of GAA, producing STLs with four distinct stereo-configurations (12,6α-, 12,6β-, 12,8α-, and 12,8β-olide derivatives of GAA) in nature. Curiously, two configurations of STLs (C12,8α and C12,8β) are simultaneously present in the Chinese medicinal plant, Inula hupehensis...
October 31, 2017: Plant Journal: for Cell and Molecular Biology
Ning Zhang, Zhixue Wang, Zhilong Bao, Leiyun Yang, Dianxing Wu, Xiaoli Shu, Jian Hua
Emerging evidence indicates a close connection between cell cycle progression and plant immune responses. In Arabidopsis, MODIFIER OF snc1-1 (MOS1) modulates a number of processes including endoreduplication and plant disease resistance, but the molecular mechanism underlying this modulation was not fully understood. Here, we provide biochemical and genetic evidence that TEOSINTE BRANCHED 1, CYCLOIDEA, PCF1 (TCP) transcription factors TCP15 and its homologs are mediators of MOS1 function in immune response and likely cell cycle control as well...
October 31, 2017: Plant Journal: for Cell and Molecular Biology
Hugo Mélida, Sara Sopeña-Torres, Laura Bacete, María Garrido-Arandia, Lucía Jordá, Gemma López, Antonio Muñoz, Luis F Pacios, Antonio Molina
Fungal cell walls, essential for environmental adaptation and host colonization, have been evolutionarily selected by plants and animals as a source of Microbe-Associated Molecular Patterns (MAMPs) that upon recognition by host Pattern Recognition Receptors (PRRs) trigger immune responses conferring disease resistance. Chito-oligosaccharides (β-1,4-N-acetylglucosamine oligomers, (GlcNAc)n ) are the only glycosidic structures from fungal walls well-shown to function as MAMPs in plants. Perception of (GlcNAc)4-8 by Arabidopsis involves CERK1, LYK4 and LYK5, three of the eight members of LysM PRR family...
October 30, 2017: Plant Journal: for Cell and Molecular Biology
Michal Pyc, Yingqi Cai, Satinder K Gidda, Olga Yurchenko, Sunjung Park, Franziska K Kretschmar, Till Ischebeck, Oliver Valerius, Gerhard H Braus, Kent D Chapman, John M Dyer, Robert T Mullen
Cytoplasmic lipid droplets (LDs) are found in all types of plant cells where they are derived from the endoplasmic reticulum and function as a repository for neutral lipids, as well as serving in lipid remodelling and signalling. However, the mechanisms underlying the formation, steady-state maintenance and turnover of plant LDs, particularly in non-seed tissues, are relatively unknown. Previously, we showed that the LD-Associated Proteins (LDAPs) are a family of plant-specific, LD surface-associated coat proteins that are required for proper LD biogenesis and neutral lipid homeostasis in vegetative tissues...
October 30, 2017: Plant Journal: for Cell and Molecular Biology
Dolores Córdoba-Cañero, Valérie Cognat, Rafael R Ariza, Teresa Roldán Arjona, Jean Molinier
DNA methylation contributes to key regulatory processes during plant development by controlling gene expression. Genomic methylation patterns are dynamic and must be properly maintained and/or re-established upon DNA replication and active removal, therefore requiring sophisticated control mechanisms. Here, we identified a direct interplay between the DNA repair factor DNA DAMAGE BINDING protein 2 (DDB2) and the ROS1-mediated active DNA demethylation pathway in Arabidopsis thaliana. We showed that DDB2 forms a complex with ROS1 and AGO4 and that they act at ROS1 locus to modulate DNA methylation levels and therefore ROS1 expression...
October 27, 2017: Plant Journal: for Cell and Molecular Biology
Kim Osman, Jianhua Yang, Elisabeth Roitinger, Christophe Lambing, Stefan Heckmann, Elaine Howell, Maria Cuacos, Richard Imre, Gerhard Dürnberger, Karl Mechtler, Sue Armstrong, F Chris H Franklin
During meiosis, formation of crossovers (COs) generates genetic variation and provides physical links essential for accurate chromosome segregation. COs occur in the context of a proteinaceous chromosome axis. The transcriptomes and proteomes of anthers and meiocytes comprise several thousand genes and proteins but due to the level of complexity relatively few have been functionally characterised. Understanding of the physical and functional interactions between meiotic proteins is also limited. Here we use affinity proteomics to analyse the proteins that are associated with the meiotic chromosome axis protein, ASY1, in Brassica oleracea anthers and meiocytes...
October 27, 2017: Plant Journal: for Cell and Molecular Biology
Wenwei Xiong, Chunlei Wang, Xiangbo Zhang, Qinghua Yang, Ruixin Shao, Jinsheng Lai, Chunguang Du
The complex interactions between transcription factors (TFs) and their target genes in a spatially and temporally specific manner are crucial to all cellular processes. Reconstruction of gene regulatory networks (GRNs) from gene expression profiles can help to decipher TF-gene regulations in a variety of contexts. However, the inevitable prediction errors of GRNs hinder optimal data mining of RNA-Seq transcriptome profiles. Here we perform an integrative study of maize seed development in order to identify key genes in a complex developmental process...
October 26, 2017: Plant Journal: for Cell and Molecular Biology
Jitao Liu, Youwei Fan, Jing Zou, Yiqun Fang, Linghao Wang, Meng Wang, Xinqiang Jiang, Yiqing Liu, Junping Gao, Changqing Zhang
Plants often develop the capacity to tolerate moderate and reversible environmental stresses, such as drought, and to re-establish normal development once the stress has been removed. An example of this phenomenon is provided by cut rose (Rosa hybrida) flowers, which experience typical reversible dehydration stresses during the postharvest handling after harvesting at the bud stages. However, the molecular mechanisms involved in rose flower dehydration tolerance are not known. Here, we characterized a dehydration- and abscisic acid (ABA)-induced ferritin gene (RhFer1)...
October 26, 2017: Plant Journal: for Cell and Molecular Biology
Timothée Vincent, Audrey Vingadassalon, Elodie Ubrig, Kevin Azeredo, Ola Srour, Valérie Cognat, Stéfanie Graindorge, Thalia Salinas, Laurence Maréchal-Drouard, Anne-Marie Duchêne
Intracellular sorting of mRNAs is an essential process to regulate gene expression and protein localization. Most of mitochondrial proteins are nuclear-encoded and imported into mitochondria, through post-translational or co-translational processes. In the latter case, mRNAs are found enriched in the vicinity of mitochondria. A genome-scale analysis of mRNAs associated with mitochondria has been performed to determine plant cytosolic mRNAs targeted to the mitochondrial surface. Many messengers encoding mitochondrial proteins were found associated with mitochondria...
October 17, 2017: Plant Journal: for Cell and Molecular Biology
Yasuhito Sakuraba, Selin Bülbül, Weilan Piao, Giltsu Choi, Nam-Chon Paek
Arabidopsis EARLY FLOWERING3 (ELF3) functions in modulating light input to the circadian clock, as a component of ELF3-ELF4-LUX ARRHYTHMO (LUX) evening complex. However, the role of ELF3 in stress responses remains largely unknown. In this study, we show that ELF3 enhances plants' resilience to salt stress: ELF3-overexpressing (ELF3-OX) plants are salt-tolerant while elf3 mutants are more sensitive to salt stress. Expression of many salt stress- and senescence-associated genes are altered in elf3-1 and ELF3-OX plants compared to wild type...
October 15, 2017: Plant Journal: for Cell and Molecular Biology
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