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Plant Journal: for Cell and Molecular Biology

Lin Zhao, Minmin Li, Chongjing Xu, Xue Yang, Dongmei Li, Xue Zhao, Kuo Wang, Yinghua Li, Xiaoming Zhang, Lixue Liu, Fuquan Ding, Hailong Du, Chunsheng Wang, Jingzhe Sun, Wenbin Li
The present study screened for polymorphisms in coding and non-coding regions of the GmGBP1 gene in 278 soybean accessions with variable maturity and growth habit characteristics under natural field conditions in three different latitudes in China. The results showed that the promoter region was highly diversified compared to the coding sequence of GmGBP1. Five polymorphisms and four haplotypes were closely related to soybean flowering time and maturity through association and linkage disequilibrium analyses...
July 13, 2018: Plant Journal: for Cell and Molecular Biology
Kristian Mark P Caldo, Wei Shen, Yang Xu, Linda Hanley-Bowdoin, Guanqun Chen, Randall Weselake, M Joanne Lemieux
Diacylglycerol acyltransferase 1 catalyzes the final and committed step in the Kennedy pathway for triacylglycerol (TAG) biosynthesis, and as such, elucidating its mode of regulation is critical to understand the fundamental aspects of carbon metabolism in oleaginous crops. In this study, purified Brassica napus diacylglycerol acyltransferase 1 (BnaDGAT1) in n-dodecyl-β-D-maltopyranoside micelles was lipidated to form mixed micelles and subjected to detailed biochemical analysis. The degree of mixed micelle fluidity appeared to influence acyltransferase activity...
July 13, 2018: Plant Journal: for Cell and Molecular Biology
Fernando Pérez-Martín, Fernando J Yuste-Lisbona, Benito Pineda, Begoña García-Sogo, Iván Del Olmo, Juan de Dios Alché, Isabel Egea, Francisco B Flores, Manuel Piñeiro, José A Jarillo, Trinidad Angosto, Juan Capel, Vicente Moreno, Rafael Lozano
Pollen development is a crucial step in higher plants which not only makes possible plant fertilization and seed formation, but also determine fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther development and the lack of viable pollen formation, which led to the production of parthenocarpic fruits. Genomic analyses and the characterization of silencing lines proved that pod1 mutant phenotype relies on the tomato SlMED18 gene encoding the subunit 18 of Mediator multi-protein complex involved in RNA polymerase II transcription machinery...
July 12, 2018: Plant Journal: for Cell and Molecular Biology
Pawel Jurkiewicz, Su Melser, Mickaël Maucourt, Haitham Ayeb, Vasko Veljanovski, Lilly Maneta-Peyret, Mark Hooks, Dominique Rolin, Patrick Moreau, Henri Batoko
Translocator proteins (TSPO) are conserved membrane proteins extensively studied in mammals, but their function is still unclear. Angiosperm TSPO are transiently induced by abiotic stresses in vegetative tissues. We showed previously that constitutive expression of the Arabidopsis TSPO (AtTSPO) could be detrimental to the cell. Degradation of AtTSPO requires an active autophagy pathway. We show here that genetic modifications of TSPO expression in plant and yeast cells reduces the levels of cytoplasmic lipid droplets (LD)...
July 12, 2018: Plant Journal: for Cell and Molecular Biology
Cheng Zhang, Xiao-Dong Teng, Quan-Quan Zheng, Yan-Yun Zhao, Jie-Yang Lu, Yichuan Wang, Hongwei Guo, Zhong-Nan Yang
ETHYLENE INSENSITIVE 3 (EIN3) is a key regulator of ethylene signaling, and EIN3-BINDING F-BOX1 (EBF1) and EBF2 are responsible for EIN3 degradation. Previous report showed that the ebf1 ebf2 double homozygous mutant cannot be identified. In this work, the genetic analysis revealed that the ebf1 ebf2 female gametophyte is defective. The pollination experiment showed that ebf1 ebf2 ovules failed to attract pollen tubes. In female gametophyte/ovule, the synergid cell is responsible for pollen tube attraction...
July 12, 2018: Plant Journal: for Cell and Molecular Biology
Ana Ruiz-Gayosso, Rogelio Rodríguez-Sotres, Eleazar Martínez-Barajas, Patricia Coello
SnRK1 is a protein kinase complex that is involved in several aspects of plant growth and development. There is published data indicative of a participation of SnRK1 in the regulation of the synthesis and degradation of starch, although the molecular mechanism is not known. In this work, we performed electron microscopy to explore the in vivo localization of the regulatory and catalytic subunits that constitute the SnRK1 complex. The results indicate that all the subunits are present in the chloroplast and, in particular, the SnRK1 βγ and SnRK1 β3 subunits are associated with starch...
July 12, 2018: Plant Journal: for Cell and Molecular Biology
Feng Sun, Xiaoyan Zhang, Yun Shen, Hongchun Wang, Rui Liu, Xiaomin Wang, Dahai Gao, Yan-Zhuo Yang, Yiwei Liu, Bao-Cai Tan
Splicing of plant organellar group II introns is under accurate nuclear control that employs many nucleus-encoded protein cofactors from various families. For mitochondrial introns, only a few splicing factors have been characterized because disruption of their functions often causes embryo lethality. Here, we report the function of Empty Pericarp8 (Emp8) in the splicing of three group II introns in mitochondria, complex I biogenesis and seed development in maize. Emp8 encodes a P-subfamily pentatricopeptide repeat (PPR) protein that localizes in mitochondria...
July 12, 2018: Plant Journal: for Cell and Molecular Biology
Xin Zhang, Dan He, Yanxiang Zhao, Xilan Cheng, Wensheng Zhao, Ian A Taylor, Jun Yang, Junfeng Liu, You-Liang Peng
Fungal avirulence effectors, a key weapon utilized by pathogens to promote their infection, are recognized by immune receptors to boost host R gene-mediated resistance. Many avirulence effectors share sparse sequence homology to proteins with known functions, and their molecular and biochemical functions together with the evolutionary relationship among different members remain largely unknown. Here, crystal structure of AvrPib, an avirulence effector from Magnaporthe oryzae, were determined and showed a high degree of similarity to the M...
July 10, 2018: Plant Journal: for Cell and Molecular Biology
D Marc Jones, Rachel Wells, Nick Pullen, Martin Trick, Judith A Irwin, Richard J Morris
Polyploidy is a recurrent feature of eukaryotic evolution and has been linked to increases in complexity, adaptive radiation, and speciation. Within angiosperms, such events have occurred repeatedly in many plant lineages. Here we investigate the retention and spatio-temporal expression dynamics of duplicated genes predicted to regulate the floral transition in Brassica napus (oilseed rape, OSR). We show that flowering time genes are preferentially retained relative to other genes in the OSR genome. Using a transcriptome time series in two tissues (leaf and shoot apex) across development, we show that 67% of these retained flowering time genes are expressed...
July 10, 2018: Plant Journal: for Cell and Molecular Biology
Clara Chicois, Hélène Scheer, Shahïnez Garcia, Hélène Zuber, Jérôme Mutterer, Johana Chicher, Philippe Hammann, Dominique Gagliardi, Damien Garcia
The RNA-helicase UPF1 is a key factor of Nonsense-Mediated Decay (NMD), an mRNA decay pathway involved in RNA quality control and in the fine-tuning of gene expression. UPF1 recruits UPF2 and UPF3 to constitute the NMD core complex, which is conserved across eukaryotes. No other components of UPF1-containing RNPs are known in plants, despite its key role in regulating gene expression. Here, we report the identification of a large set of proteins that copurify with the Arabidopsis UPF1, either in a RNA-dependent or RNA-independent manner...
July 7, 2018: Plant Journal: for Cell and Molecular Biology
Jiaren Yao, Kiyoshi Mashiguchi, Adrian Scaffidi, Tomoki Akatsu, Kim T Melville, Ryo Morita, Yu Morimoto, Steven M Smith, Yoshiya Seto, Gavin R Flematti, Shinjiro Yamaguchi, Mark T Waters
Karrikins are butenolide compounds present in post-fire environments that can stimulate seed germination in many species, including Arabidopsis thaliana. Plants also produce endogenous butenolide compounds that serve as hormones, namely strigolactones (SLs). The receptor for karrikins (KARRIKIN INSENSITIVE2; KAI2) and the receptor for SLs (DWARF14; D14) are homologous proteins that share many similarities. The mode of action of D14 as a dual enzyme-receptor protein is well established, but the nature of KAI2-dependent signalling and its function as a receptor are not fully understood...
July 7, 2018: Plant Journal: for Cell and Molecular Biology
Masakazu Iwai, Melissa S Roth, Krishna K Niyogi
The chloroplast is the chlorophyll-containing organelle that produces energy through photosynthesis. Within the chloroplast is an intricate network of thylakoid membranes containing photosynthetic membrane proteins that mediate electron transport and generate chemical energy. Historically, electron microscopy has been a powerful tool to visualize the macromolecular structure and organization of thylakoid membranes. However, understanding thylakoid membrane dynamics remains elusive because electron microscopy requires fixation and sectioning...
July 7, 2018: Plant Journal: for Cell and Molecular Biology
Jiang Chang, Ting Yu, Qihong Yang, Changxing Li, Cheng Xiong, Shenghua Gao, Qingmin Xie, Fangyan Zheng, Hanxia Li, Zhendong Tian, Changxian Yang, Zhibiao Ye
Trichomes originate from epidermal cells of nearly all terrestrial plants, which are specialized unicellular or multicellular structures. Although the molecular mechanism regulating unicellular trichome formation has been extensively characterized, most of genes essential for multicellular trichome formation still remain unknown. In this study, we identified an associated locus on the long arm of chromosome 10 using genome-wide association study (GWAS) on type I trichomes of 180 diverse tomato accessions...
July 6, 2018: Plant Journal: for Cell and Molecular Biology
Agnieszka A Golicz, Prem L Bhalla, Mohan B Singh
Rice is an important cereal crop, providing staple food for over a half of the world's population and sexual reproduction resulting in grain formation underpins global food security. However, despite considerable research efforts, many of the genes, especially long intergenic non-coding RNA (lincRNA) genes involved in rice sexual reproduction remain uncharacterized. With increasing number of public resources becoming available, information from different sources can be combined to perform gene functional annotation...
July 6, 2018: Plant Journal: for Cell and Molecular Biology
Oren Tzfadia, Samuel Bocobza, Jonas Defoort, Efrat Almekias-Siegl, Sayantan Panda, Matan Levy, Veronique Storme, Stephane Rombauts, Diego Adhemar Jaitin, Hadas Keren-Shaul, Yves Van de Peer, Asaph Aharoni
High-throughput RNA sequencing has proven invaluable not only to explore gene expression, but also for both gene prediction and genome annotation. However, RNA sequencing, carried out on tens or even hundreds of samples, requires easy and cost-effective sample preparation methods using minute RNA amounts. Here, we present TranSeq, a high-throughput 3'-end sequencing procedure that requires 10- to 20-fold fewer sequence reads than the current transcriptomics procedures. TranSeq significantly reduces costs and allows a great increase in size of sample sets analyzed in a single experiment...
July 6, 2018: Plant Journal: for Cell and Molecular Biology
Ryo Mameda, Toshiyuki Waki, Yosuke Kawai, Seiji Takahashi, Toru Nakayama
Soybean (Glycine max) 5-deoxyisoflavonoids (daidzein and its conjugates) are precursors of glyceollin phytoalexins. They are also converted to equol by microbes in the human intestine, resulting in health benefits. 5-Deoxyisoflavonoids accumulate in the roots (93% mol/mol of the total root isoflavonoids) and seeds of unstressed soybean plants. Chalcone reductase (CHR) is a key enzyme mediating 5-deoxyisoflavonoid biosynthesis because it catalyzes the production of 6'-deoxychalcone via its effects on the chalcone synthase (CHS)-catalyzed reaction...
July 6, 2018: Plant Journal: for Cell and Molecular Biology
Nan Wang, Changzhi Qu, Shenghui Jiang, Zijing Chen, Haifeng Xu, Hongcheng Fang, Mengyu Su, Jing Zhang, Yicheng Wang, Wenjun Liu, Zongying Zhang, Ninglin Lu, Xuesen Chen
In plants, flavonoids play critical roles in resistance to biotic and abiotic stresses, and contribute substantially to the quality, flavor, and nutritional quality of many fruit crops. In apple (Malus × domestica), inbreeding has resulted in severe decreases in the genetic diversity and flavonoid content. During the last decade, we focused on the genetic improvement of apple using wild red-fleshed apple resources (Malus sieversii f. niedzwetzkyana). Here, we found that the MYB transcription factors (TFs) involved in the synthesis of proanthocyanidins can be classified into TT2 and PA1-types...
July 6, 2018: Plant Journal: for Cell and Molecular Biology
Li Huang, Heng Dong, Dong Zhou, Ming Li, Yanhong Liu, Fang Zhang, Yaoyao Feng, Dongliang Yu, Sue Lin, Jiashu Cao
The importance of long non-coding RNAs (lncRNAs) in plant development has been established, but a systematic analysis of lncRNAs expressed during pollen development and fertilization has been elusive. We performed a time-series of RNA-seq experiments at five developmental stages during pollen development and three different time points after pollination in Brassica rapa and identified 12,051 putative lncRNAs. A comprehensive view of dynamic lncRNA expression networks underpinning pollen development and fertilization was provided...
July 5, 2018: Plant Journal: for Cell and Molecular Biology
Morgane Roth, Ana M Florez-Rueda, Margot Paris, Thomas Städler
Genomic imprinting is a conspicuous feature of the endosperm, a triploid tissue nurturing the embryo and synchronizing angiosperm seed development. An unknown subset of imprinted genes (IGs) is critical for successful seed development and should have highly conserved functions. Recent genome-wide studies have found limited conservation of IGs among distantly related species, but there is a paucity of data from closely related lineages. Moreover, most studies focused on model plants with nuclear endosperm development, and comparisons with properties of IGs in cellular-type endosperm development are lacking...
June 28, 2018: Plant Journal: for Cell and Molecular Biology
Stuart K Meier, Nicolette Adams, Michael Wolf, Kevin Balkwill, A Muthama Muasya, Christoph A Gehring, Jacqueline M Bishop, Robert A Ingle
The majority of metal hyperaccumulating plants accumulate nickel, yet the molecular basis of Ni hyperaccumulation is not well understood. We chose Senecio coronatus to investigate this phenomenon as this species displays marked variation in shoot Ni content across ultramafic outcrops in the Barberton Greenstone Belt (South Africa), thus allowing an intra-specific comparative approach to be employed. No correlation between soil and shoot Ni contents was observed, suggesting that this variation has a genetic rather than environmental basis...
June 27, 2018: Plant Journal: for Cell and Molecular Biology
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