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Journal of Assisted Reproduction and Genetics

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https://www.readbyqxmd.com/read/30535641/correlation-of-human-telomerase-reverse-transcriptase-single-nucleotide-polymorphisms-with-in-vitro-fertilisation-outcomes
#1
Kailing Dai, Hongmei Xu, Nengyong Ouyang, Ying Li, Ping Yuan, Liangan Wang, Xiaomiao Zhao, Wenjun Wang
PURPOSE: To explore whether it is possible to predict the number and quality of the embryo using a few particular hTERT SNPs. METHODS: We included 997 Han Chinese women who were genetically unrelated and underwent assisted reproduction using IVF from September 2014 to December 2015. DNA was genotyped by using TaqMan real-time quantitative PCR. RESULTS: Among the 997 patients, individuals with the CC genotype of rs2075786 had a significantly lower number of good-quality embryos than those with the TT+TC genotypes...
December 10, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30519898/transdermal-versus-oral-estrogen-clinical-outcomes-in-patients-undergoing-frozen-thawed-single-blastocyst-transfer-cycles-without-gnrha-suppression-a-prospective-randomized-clinical-trial
#2
Semra Kahraman, Caroline Pirkevi Çetinkaya, Yucel Sahin, Gokalp Oner
PURPOSE: To conduct a non-inferiority study to compare the clinical outcomes of transdermal estrogen patch and oral estrogen in patients undergoing frozen-thawed single blastocyst transfer non-donor cycles without GnRHagonist (GnRHa) suppression. METHODS: A total of 317 women with irregular menses or anovulatory cycle undergoing frozen-thawed embryo transfer (FET) non-donor cycles without GnRHa suppression were involved in a prospective randomized clinical trial between May 2017 and October 2017...
December 5, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30515618/association-between-parental-anthropometric-measures-and-the-outcomes-of-intracytoplasmic-sperm-injection-cycles
#3
Amanda Setti, Gabriela Halpern, Daniela Braga, Rita Figueira, Assumpto Iaconelli, Edson Borges
PURPOSE: To study the association between body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR), and response to controlled ovarian stimulation, semen quality, and intracytoplasmic sperm injection (ICSI) outcomes. METHODS: This prospective observational study included data from 402 couples undergoing ICSI between January/2010-September/2016. Participants had their weight, height, waist, and hip measured before ICSI treatment...
December 5, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30506186/bringing-perspective-to-the-next-wave-of-human-arts
#4
EDITORIAL
David F Albertini
No abstract text is available yet for this article.
December 1, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30483911/rare-genetic-variants-potentially-involved-in-ovarian-hyperstimulation-syndrome
#5
Katrien Stouffs, Sari Daelemans, Samuel Santos-Ribeiro, Sara Seneca, Alexander Gheldof, Ali Sami Gürbüz, Michel De Vos, Herman Tournaye, Christophe Blockeel
PURPOSE: We aim to investigate whether there is a genetic predisposition in women who developed ovarian hyperstimulation syndrome (OHSS) after GnRH antagonist protocol with GnRH agonist trigger and freeze-all approach. METHODS: Four patients with OHSS after GnRH agonist trigger and freeze-all approach were gathered from the worldwide patient population. These patients were analyzed through Whole Exome Sequencing. In this study known causes of OHSS were investigated and new causes present in at least two individuals were searched for...
November 27, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30478807/fertility-preservation-in-women-with-malignancies-the-accuracy-of-antral-follicle-count-collected-randomly-during-the-menstrual-cycle-in-predicting-the-number-of-oocytes-retrieved
#6
Francesca Filippi, Fabio Martinelli, Alessio Paffoni, Marco Reschini, Francesco Raspagliesi, Edgardo Somigliana
PURPOSE: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. A standardized random start protocol was used for all women...
November 26, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30470961/effect-of-intra-ovarian-injection-of-mesenchymal-stem-cells-in-aged-mares
#7
Sicilia T Grady, Ashlee E Watts, James A Thompson, M Cecilia T Penedo, Kranti Konganti, Katrin Hinrichs
PURPOSE: This study aims to determine if intra-ovarian injection of bone marrow-derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females. METHODS: Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing...
November 23, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30470960/chromosomal-abnormalities-in-couples-with-recurrent-spontaneous-miscarriage-a-21-year-retrospective-study-a-report-of-a-novel-insertion-and-a-literature-review
#8
Zouhair Elkarhat, Zineb Kindil, Latifa Zarouf, Lunda Razoki, Jamila Aboulfaraj, Chadli Elbakay, Sanaa Nassereddine, Boubker Nasser, Abdelhamid Barakat, Hassan Rouba
PURPOSE: The aim of this study is to evaluate the frequency and nature of chromosomal abnormalities in Moroccan couples with recurrent spontaneous miscarriage (RSM). In addition, the data were compared with those reported elsewhere in order to give a global estimation of chromosomal abnormalities frequencies. METHODS: The study was performed for all couples with RSM who were referred to the cytogenetic department, Pasteur Institute of Morocco, from different hospitals in Morocco between 1996 and 2016...
November 23, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30467617/can-peri-ovulatory-putrescine-supplementation-improve-egg-quality-in-older-infertile-women
#9
REVIEW
Yong Tao, Alina Tartia, Maralee Lawson, Mary B Zelinski, Wei Wu, Jia-Yin Liu, Johan Smitz, Marie-Claude Léveillé, Arthur Leader, Hongmei Wang, Timothy Ramsay, X Johné Liu
The aging-related decline in fertility is an increasingly pressing medical and economic issue in modern society where women are delaying family building. Increasingly sophisticated, costly, and often increasingly invasive, assisted reproductive clinical protocols and laboratory technologies (ART) have helped many older women achieve their reproductive goals. Current ART procedures have not been able to address the fundamental problem of oocyte aging, the increased rate of egg aneuploidy, and the decline of developmental potential of the eggs...
November 22, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30443692/simulated-physiological-oocyte-maturation-has-side-effects-on-bovine-oocytes-and-embryos
#10
Eduardo M Razza, Hanne S Pedersen, Lotte Stroebech, Patricia K Fontes, Haja N Kadarmideen, Henrik Callesen, Maria Pihl, Marcelo F G Nogueira, Poul Hyttel
PURPOSE: Oocyte maturation is a complex process involving nuclear and cytoplasmic modulations, during which oocytes acquire their ability to become fertilized and support embryonic development. The oocyte is apparently "primed" for maturation during its development in the dominant follicle. As bovine oocytes immediately resume meiosis when cultured, it was hypothesized that delaying resumption of meiosis with cyclic nucleotide modulators before in vitro maturation (IVM) would allow the oocytes to acquire improved developmental competence...
November 16, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30443691/chromosome-constitution-of-equal-sized-three-cell-embryos-using-next-generation-sequencing-technology
#11
Minyue Ma, Shihui Zhang, Chongzhao Lu, Shuling Wang, Yuanqing Yao, Hongmei Peng
PURPOSE: To study the chromosome constitution of equal-sized three-cell embryo. METHODS: We determined the chromosome constitution of 105 blastomeres from 35 embryos using multiple annealing and looping-based amplification cycles (MALBAC) together with NGS sequencing technology. Chromosomal copy number variation (CNV) analysis was successfully performed in 27 embryos. We also analyzed radius, perimeter, area, and volume of each blastomere to explore the possibility of selecting the normal embryos...
November 16, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30430314/treatment-of-female-rhesus-macaques-with-a-somatostatin-receptor-antagonist-that-increases-oocyte-fertilization-rates-without-affecting-post-fertilization-development-outcomes
#12
Alison Y Ting, Melinda J Murphy, Pablo Arriagada, Jean-Pierre Gotteland, Jon D Hennebold
PURPOSE: To determine the effects of PGL1001, a somatostatin receptor isoform-2 (SSTR-2) antagonist, on ovarian follicle development, oocyte fertilization, and subsequent embryo developmental potential in the rhesus macaque. METHODS: Cycling female rhesus macaques (N = 8) received vehicle through one menstrual (control) cycle, followed by daily injections of PGL1001, a SSTR-2 antagonist, for three menstrual (treatment) cycles. Main endpoints include overall animal health and ovarian hormones (e...
November 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30430313/single-blastomeres-as-a-source-of-mouse-embryonic-stem-cells-effect-of-genetic-background-medium-supplements-and-signaling-modulators-on-derivation-efficiency
#13
Marta Vila-Cejudo, Ot Massafret, Josep Santaló, Elena Ibáñez
PURPOSE: To assess the role of the genetic background, the culture medium supplements, and the presence of modulators of signaling pathways on mouse embryonic stem cell derivation from single blastomeres from 8-cell embryos. METHODS: Mice from permissive and non-permissive genetic backgrounds, different culture media supplements, knockout serum replacement (KSR) and N2B27, and the presence or absence of 2i treatment were used to derive mouse embryonic stem cells (mESC) from single blastomeres isolated from 8-cell embryos and from control embryos at the blastocyst stage...
November 14, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30421343/time-lapse-imaging-reveals-delayed-development-of-embryos-carrying-unbalanced-chromosomal-translocations
#14
Hadar Amir, Shiri Barbash-Hazan, Yael Kalma, Tsvia Frumkin, Mira Malcov, Nivin Samara, Joseph Hasson, Adi Reches, Foad Azem, Dalit Ben-Yosef
PURPOSE: The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy. METHODS: The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5...
November 12, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30421342/the-model-of-genetic-compartments-a-new-insight-into-reproductive-genetics
#15
REVIEW
X Vendrell, M J Escribà
Currently, we are witnessing revolutionary advances in the analytical power of genetic tools. An enormous quantity of data can now be obtained from samples; however, the translation of genetic findings to the general status of individuals, or their offspring, should be done with caution. This is especially relevant in the reproductive context, where the concepts of "transmission" and "inheritability" of a trait are crucial. Against this background, we offer new insight based on a systemic view of genetic constitution in the compartmentalized organism, that is, the human body...
November 12, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30421341/business-models-and-provider-satisfaction-in-in-vitro-fertilization-centers-in-the-usa
#16
J E McLaughlin, J F Knudtson, R S Schenken, N S Ketchum, J A Gelfond, T A Chang, R D Robinson
PURPOSE: The number of in vitro fertilization (IVF) cycles is increasing and the majority of patients undergoing IVF pay out of pocket. Reproductive endocrinology and infertility practitioners employ different business models to help create financial pathways for patients needing IVF but details regarding the different types of business models being used and physician satisfaction with those models have not been described previously. METHODS: A cross-sectional survey was sent to members of the Society of Reproductive Endocrinology and Infertility...
November 12, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30415469/semen-parameters-on-the-day-of-oocyte-retrieval-predict-low-fertilization-during-conventional-insemination-ivf-cycles
#17
A L Harris, J C Vanegas, E Hariton, P Bortoletto, M Palmor, L A Humphries, C Tanrikut, J E Chavarro, A K Styer
PURPOSE: Poor fertilization during conventional IVF is difficult to predict in the absence of abnormal semen parameters; large-scale studies are lacking. The purpose of this study is to evaluate factors associated with low fertilization rates in conventional insemination IVF cycles. METHODS: A retrospective cohort study evaluating demographic, reproductive evaluation, and IVF cycle characteristics to identify predictors of low fertilization (defined as 2PN/MII ≤ 30% per cycle)...
November 10, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30415468/limited-relationships-between-reactive-oxygen-species-levels-in-culture-media-and-zygote-and-embryo-development
#18
Kuo-Chung Lan, Yi-Chi Lin, Yung-Chiao Chang, Hsin-Jung Lin, Yi-Ru Tsai, Hong-Yo Kang
PURPOSE: Reactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early human embryonic parameters and levels of ROS in culture media. METHODS: This prospective study was conducted with 2633 spent culture media collected from patients undergoing conventional IVF (n = 101) and intracytoplasmic sperm injection (ICSI) (n = 60)...
November 10, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30415467/somatic-offloading-during-sperm-maturation-shining-light-on-the-extragenomic-paternal-dowry
#19
EDITORIAL
David F Albertini
No abstract text is available yet for this article.
November 10, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30411275/chromosomal-scan-of-single-sperm-cells-by-combining-fluorescence-activated-cell-sorting-and-next-generation-sequencing
#20
Quoc Ty Tran, Tatjana Jatsenko, Olev Poolamets, Olga Tšuiko, Dmitri Lubenets, Tiia Reimand, Margus Punab, Maire Peters, Andres Salumets
PURPOSE: The purpose of this study was to develop a feasible approach for single sperm isolation and chromosome analysis by next-generation sequencing (NGS). METHODS: Single sperm cells were isolated from semen samples of normozoospermic male and an infertile reciprocal translocation (RcT) carrier with the 46,XY,t(7;13)(p12;q12.1) karyotype using the optimized fluorescence-activated cell sorting (FACS) technique. Genome profiling was performed using NGS. RESULTS: Following whole-genome amplification, NGS, and quality control, the final chromosome analysis was performed on 31 and 6 single cell samples derived from the RcT carrier and normozoospermic male, respectively...
November 9, 2018: Journal of Assisted Reproduction and Genetics
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