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Journal of Assisted Reproduction and Genetics

Ronit Abir, Benjamin Fisch, Noa Fisher, Nivin Samara, Galit Lerer-Serfaty, Roei Magen, Michal Herman-Edelstein, Avi Ben-Haroush, Anat Stein, Raoul Orvieto
PURPOSE: To investigate if needle-immersed vitrification or slow-freezing yields better implantation results for human ovarian tissue and which method benefits more when combined with the "improvement protocol" of host melatonin treatment and graft incubation with biological glue + vitamin E + vascular endothelial growth factor-A. METHODS: Human ovarian tissue was preserved by needle-immersed vitrification or slow-freezing and transplanted into immunodeficient mice, either untreated (groups A and C, respectively) or treated with the improvement protocol (groups B and D, respectively)...
March 18, 2017: Journal of Assisted Reproduction and Genetics
Phillip A Romanski, Alexander Melamed, Kevin M Elias, Aleksandar K Stanic, Raymond M Anchan
PURPOSE: Ovarian torsion is a surgical emergency that can be clinically challenging to diagnose. Patients who have received assisted reproductive technologies (ART) are a subset of women with an increased risk for torsion. As the ART population continues to increase, there is a need to delineate risk factors for the development of ovarian torsion in this unique population. A pilot study was performed to determine the proportion of patients with suspected ovarian torsion who have received ART and to identify possible diagnostic biomarkers for ovarian torsion among these patients...
March 15, 2017: Journal of Assisted Reproduction and Genetics
Samer Tannus, Weon-Young Son, Michael Haim Dahan
PURPOSE: The purpose of this study was to investigate reproductive outcomes following elective single blastocyst transfer (eSBT) compared with those of double blastocyst transfer (DBT) in advanced maternal age. METHODS: This was a retrospective cohort study performed at an academic fertility center. All women aged 40 and over for whom in vitro fertilization (IVF) cycles were performed and in whom embryo culture was extended to the blastocyst stage were reviewed for possible inclusion...
March 15, 2017: Journal of Assisted Reproduction and Genetics
Yue Lv, Changfa Sun, Ye Tian, Shigang Zhao, Yuehong Bian, Lei Cheng, Mei Sun, Hong-Bin Liu, Han Zhao, Jinlong Ma
PURPOSE: This study aims to ascertain whether an association exists between hepatocyte nuclear factor 1 alpha (HNF1A) and polycystic ovary syndrome (PCOS). METHODS: One thousand one hundred thirty-eight PCOS and 1125 healthy control Han Chinese women were recruited from Reproductive Hospital Affiliated to Shandong University. Serum hormone, blood lipid level, and genomic DNA were obtained from the peripheral blood for this research. Two single-nucleotide polymorphisms (SNPs)-rs2393791 and rs7305618-located in HNF1A were genotyped using the Sequenom MassARRAY system...
March 15, 2017: Journal of Assisted Reproduction and Genetics
Akansha Tiwari, Merih Tekcan, Leyla Sati, William Murk, Jill Stronk, Gabor Huszar
PURPOSE: Our aim was the development of a safe sperm cryopreservation New Media (NM), composed of consistent and reproducible components devoid of any animal origin, and evaluation of NM in terms of its effect on sperm structure and function as compared to regularly used yolk media (TYM) (Irvine Scientific). METHODS: We evaluated patient semen samples and cryopreserved them in duplicates in either NM or TYM. The samples were cryopreserved for either a short term of 1 week or long term of 1 month prior to thawing...
March 9, 2017: Journal of Assisted Reproduction and Genetics
Sabela García-Oro, María Isabel Rey, Marta Rodríguez, Ángel Durán, Roque Devesa, Diana Valverde
PURPOSE: We evaluated the relationship between meiotic spindle characteristics and in vitro fertilization cycle outcome. METHODS: Five hundred sixty-nine oocytes from 86 in vitro fertilization cycles were analyzed for fertilization and subsequent implantation rates. Oocytes were assessed for maturation status. The oocytes and embryos were cultured in sequential and nonsequential media (G Series, Vitrolife, Sweden) and incubated in 6% CO2, 5% O2 at 37 °C. Two hours following oocyte decumulation (38-39 h post-hCG/GnRH administration) and prior to microinjection, the structure of the meiotic spindle was assessed using the Oosight Imaging System (CRI, UK)...
March 9, 2017: Journal of Assisted Reproduction and Genetics
L Tamburino, S La Vignera, V Tomaselli, R A Condorelli, L M Mongioì, A E Calogero
PURPOSE: The FSHB gene -211G/T polymorphism has been reported to modulate gene expression and to cause inter-individual differences in FSH serum levels in men. This study was undertaken to assess the functional relevance of this polymorphism on gonadotropin and total testosterone serum levels and sperm parameters in men from Eastern Sicily (Italy). METHODS: To accomplish this, 200 men with abnormal conventional sperm parameters or normozoospermia (according to the parameters of WHO 2010) were genotyped by TaqMan Assay...
March 9, 2017: Journal of Assisted Reproduction and Genetics
Amr Rafat Elhamamsy
Genomic imprinting is a complex epigenetic process that contributes substantially to embryogenesis, reproduction, and gametogenesis. Only small fraction of genes within the whole genome undergoes imprinting. Imprinted genes are expressed in a monoallelic parent-of-origin-specific manner, which means that only one of the two inherited alleles is expressed either from the paternal or maternal side. Imprinted genes are typically arranged in clusters controlled by differentially methylated regions or imprinting control regions...
March 9, 2017: Journal of Assisted Reproduction and Genetics
Alina M Mahfoudh, Jeong H Moon, Sara Henderson, Elena Garcia-Cerrudo, Weon-Young Son, Michael H Dahan
PURPOSE: The goals of this study are to analyze the clinical pregnancy rate as a function of the pre-intracytoplasmic sperm injection (ICSI) oocyte spindle angle and determine factors which can be associated with different spindle angles, if clinically relevant. METHODS: Fifty-eight patients, who underwent their first ICSI cycle from January to December 2013, were included. Eight hundred thirty oocytes were collected, and 648 were metaphase II (MII) on retrieval day...
February 28, 2017: Journal of Assisted Reproduction and Genetics
David F Albertini
No abstract text is available yet for this article.
February 28, 2017: Journal of Assisted Reproduction and Genetics
Natalia Trpchevska, Ivanka Dimova, Tatyana Arabadji, Tanya Milachich, Svetlana Angelova, Magdalena Dimitrova, Mariela Hristova-Savova, Petya Andreeva, Tania Timeva, Atanas Shterev
Complex chromosome translocations are structural chromosomal rearrangements involving three or more chromosomes and more than two breakpoints. A complex chromosome rearrangement was detected in a phenotypically normal female patient that was referred to the hospital for genetic counseling due to reproductive failure. A cytogenetic evaluation was performed, according to standard method of chromosomal analysis, using G-banding technique. The patient's karyotype showed a balanced complex chromosome rearrangement (BCCR) involving chromosomes 1, 8, and 11 with three breakpoints 1p31, 8q13, and 11q23...
February 24, 2017: Journal of Assisted Reproduction and Genetics
Chloë Shaw-Jackson, Mélissa Capraro, Lieveke Ameye, Jean Vandromme, Yannick Manigart, Serge Rozenberg, Candice Autin
PURPOSE: The aim of this study was to investigate whether infection of women by the hepatitis C virus (HCV) reduces the chance of conceiving after in vitro fertilization (IVF). METHODS: We performed a retrospective blind matched case-control study where IVF outcomes for the first 37 cycles of HCV sero-positive women were compared to those of 107 cycles of an uninfected control group. Our results were included in a systematic literature review. RESULTS: Out of five eligible studies, ours included, three observed an impact of HCV infection, though at various levels including response to stimulation, fertilization, implantation, and pregnancy rates...
February 24, 2017: Journal of Assisted Reproduction and Genetics
Vrushali Sagare-Patil, Rashmi Bhilawadikar, Mosami Galvankar, Kusum Zaveri, Indira Hinduja, Deepak Modi
PURPOSE: The aims of this paper were to study whether heat shock protein 90 (HSP90) is a regulator of sperm functions and to determine its association with oligoasthenozoospermia. METHODS: The levels of HSP90 in sperm lysates were measured by ELISA. Localization of HSP90 and its isoforms was evaluated by immunofluorescence. Sperm motility and kinetics were assessed by computer-assisted sperm analysis. Acrosome reaction was determined by lectin staining. RESULTS: The levels of HSP90 were lower in oligoasthenozoospermic men and correlated positively with the number of motile spermatozoa...
February 24, 2017: Journal of Assisted Reproduction and Genetics
Thi Ngoc Lan Vuong, M T Ho, T Q Ha, M Brehm Jensen, C Yding Andersen, P Humaidan
PURPOSE: A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and top-quality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression...
February 14, 2017: Journal of Assisted Reproduction and Genetics
Miguel Russo, Senem Ates, Talya Shaulov, Michael H Dahan
PURPOSE: Maternal obesity has been shown to affect reproductive function and pregnancy outcomes following in vitro fertilization. More recently, studies have demonstrated lower live birth rates after single blastocyst transfer (SBT) in patients who are overweight or obese. However, the impact of morbid obesity on pregnancy outcomes after SBT has not been well elucidated. The present study aimed to determine whether morbid obesity has a detrimental impact on pregnancy outcomes after SBT in a North American population...
February 11, 2017: Journal of Assisted Reproduction and Genetics
Shuo-Ping Zhang, Chang-Fu Lu, Fei Gong, Ping-Yuan Xie, Liang Hu, Shun-Ji Zhang, Guang-Xiu Lu, Ge Lin
PURPOSE: We aimed to determine the developmental potential of human reconstructed oocytes after polar body genome transfer (PBT) and to report the case of a woman with multiple cycles of severe embryo fragmentation. METHODS: Fresh and cryopreserved first polar bodies (PB1s) were transferred to enucleated metaphase II oocytes (PB1T), while fresh PB2s were removed from fertilized oocytes and used instead of the female pronucleus in donor zygotes. Reconstructed oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured to blastocyst...
February 11, 2017: Journal of Assisted Reproduction and Genetics
Shu Hashimoto, Naoharu Morimoto, Masaya Yamanaka, Hiroshi Matsumoto, Takayuki Yamochi, Hiroya Goto, Masayasu Inoue, Yoshiharu Nakaoka, Hiroaki Shibahara, Yoshiharu Morimoto
PURPOSE: The oxygen consumption rates (OCRs) in mice and cattle have been reported to change during preimplantation embryogenesis. On the other hand, mitochondrial DNA (mtDNA) copy number has been shown to be unchanged in mice and changed in cattle and pigs. The interactions between mitochondrial functions and mtDNA copy numbers in human embryos during preimplantation development remain obscure. METHODS: Sixteen oocytes and 100 embryos were used to assess mtDNA copy numbers and OCR...
February 11, 2017: Journal of Assisted Reproduction and Genetics
Gerard Letterie
OBJECTIVE: Medical malpractice claims vary by specialty. Contributory factors to malpractice in reproductive endocrinology and infertility (REI) are not well defined. We sought to determine claims' frequency, basis of claims, and outcomes of settled claims in REI. DESIGN: This is a retrospective, descriptive review of 10 years of claims. SETTING: The setting is private practices. MATERIALS AND METHODS: Claims were monitored within one malpractice carrier between 2006 and 2015 covering 10 practices and 184,015 IVF cycles...
February 11, 2017: Journal of Assisted Reproduction and Genetics
Ronit Machtinger, Rodosthenis S Rodosthenous, Michal Adir, Abdallah Mansour, Catherine Racowsky, Andrea A Baccarelli, Russ Hauser
PURPOSE: The purpose of this study is to determine the profile of extracellular microRNAs (exmiRNAs) in follicular fluid (FF) and explore their association with fertilization potential and embryo quality. METHODS: We collected FF from single follicles containing mature oocytes from 40 women undergoing IVF and we screened for the expression of 754 exmiRNAs in FF using the TaqMan OpenArray® qPCR platform. To determine the association of exmiRNAs and IVF outcomes, we compared their expression levels in FF samples that differ by fertilization status (normally, abnormally, and failed to fertilize) and embryo quality (top vs...
February 10, 2017: Journal of Assisted Reproduction and Genetics
Jin Huang, Liying Yan, Sijia Lu, Nan Zhao, Jie Qiao
PURPOSE: The purpose of this study is to explore which part of the trophectoderm best represents the inner cell mass after aCGH analysis. METHODS: Fifty-one preimplantation genetic diagnosis/preimplantation genetic screening of abnormal blastocysts diagnosed by array comparative genomic hybridization were included in this study. Blastocysts were thawed, incubated for 3 to 4 h, and then biopsied. Four regions were biopsied per blastocyst, including the inner cell mass (ICM), trophectoderm (TE) cells opposite the ICM, TE cells at the upper right of the ICM, and TE cells at the lower right of the ICM...
February 10, 2017: Journal of Assisted Reproduction and Genetics
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