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Journal of Assisted Reproduction and Genetics

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https://www.readbyqxmd.com/read/28825164/the-mutation-free-embryo-for-in-vitro-fertilization-selected-by-malbac-pgd-resulted-in-a-healthy-live-birth-from-a-family-carrying-pkd-1-mutation
#1
Wen Li, Yiyi Ma, Shengqiang Yu, Ningxia Sun, Liang Wang, Dongping Chen, Guijiang Yang, Sijia Lu, Yangyang Li, Bo Yang, Changlin Mei
BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD, autosomal dominant PKD or adult-onset PKD) is the most prevalent and potentially lethal kidney disease that is hereditary and lacks effective treatment. Preimplantation genetic diagnosis (PGD) of embryos in assistant reproductive technology (ART) helps to select mutation-free embryos for blocking ADPKD inheritance from the parents to their offspring. However, there are multiple pseudogenes in the PKD1 coding region, which make blocking ADPKD inheritance by PGD complicated and difficult...
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28825156/correlation-between-follicular-fluid-levels-of-srage-and-vitamin-d-in-women-with-pcos
#2
Deepika Garg, Richard Grazi, Geralyn M Lambert-Messerlian, Zaher Merhi
PURPOSE: The pro-inflammatory advanced glycation end products (AGEs) and their anti-inflammatory soluble receptors, sRAGE, play a role in the pathogenesis of PCOS. There is a correlation between vitamin D (vit D) and sRAGE in the serum, whereby vit D replacement increases serum sRAGE levels in women with PCOS, thus incurring a protective anti-inflammatory role. OBJECTIVE: This study aims to compare levels of sRAGE, N-carboxymethyl-lysine (CML; one of the AGEs), and 25-hydroxy-vit D in the follicular fluid (FF) of women with or without PCOS, and to evaluate the correlation between sRAGE and 25-hydroxy-vit D in the FF...
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28825151/the-role-of-gene-polymorphisms-and-amh-level-in-prediction-of-poor-ovarian-response-in-egyptian-women-undergoing-ivf-procedure
#3
Tarek M K Motawi, Sherine M Rizk, Nadine W Maurice, Ahmed Mohamed Maged, Ayman N Raslan, Ahmed H Sawaf
OBJECTIVE: The aim of this study is to assess the role of AMH in prediction of poor ovarian response as well as the relation between ESR 2 (+ 1730G>A) (rs4986938) and FSHR p.Thr(307)Ala (c.919A>G, rs6165) SNPs and the poor ovarian response in Egyptian women undergoing IVF procedure. Discovering the genetic variants associated with ovarian response is an important step towards individualized pharmacogenetic protocols of ovarian stimulation. METHODS: We performed a prospective study on 216 young women with unexplained infertility...
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28823065/elevated-serum-estradiol-levels-in-artificial-autologous-frozen-embryo-transfer-cycles-negatively-impact-ongoing-pregnancy-and-live-birth-rates
#4
Rani Fritz, Sangita Jindal, Heather Feil, Erkan Buyuk
PURPOSE: The aim of this study is to evaluate the correlation between serum estradiol (E2) levels during artificial autologous frozen embryo transfer (FET) cycles and ongoing pregnancy/live birth rates (OP/LB). METHODS: A historical cohort study was conducted in an academic setting in order to correlate peak and average estradiol levels with ongoing pregnancy/live birth rates for all autologous artificial frozen embryo transfer cycles performed from 1/2011 to 12/2014...
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28823043/educating-an-oocyte-the-complex-dialogue-between-mentor-and-mentee
#5
EDITORIAL
David F Albertini
No abstract text is available yet for this article.
August 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28822010/vitrification-of-human-immature-oocytes-before-and-after-in-vitro-maturation-a-review
#6
REVIEW
Mohammad Ali Khalili, Abbas Shahedi, Sareh Ashourzadeh, Stefania Annarita Nottola, Guido Macchiarelli, Maria Grazia Palmerini
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing...
August 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28815355/egg-donation-of-vitrified-oocytes-bank-produces-similar-pregnancy-rates-by-blastocyst-transfer-when-compared-to-fresh-cycle
#7
Thais S Domingues, Ana Paula Aquino, Bruna Barros, Raquel Mazetto, Mariana Nicolielo, Carolina M Kimati, Talita Devecchi, Tatiana C S Bonetti, Paulo C Serafini, Eduardo L A Motta
PURPOSE: Advances in reproductive techniques, mainly the introduction of oocyte vitrification, have provided the opportunity to conceive from oocyte banks. The aim of this study was to compare the clinical outcomes of fresh and vitrified oocytes in an egg donation program following blastocyst transfer. METHODS: This retrospective observational study included 504 oocyte donation cycles. All donor women were younger than 30 years of age. The recipient cycles were divided into two groups: fresh oocytes (n = 78) or vitrified oocytes (n = 426)...
August 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28801781/acute-pain-crisis-in-a-patient-with-sickle-cell-disease-undergoing-ovarian-simulation-for-fertility-preservation-prior-to-curative-stem-cell-transplantation-case-report-and-literature-review
#8
Michelle Matthews, Rebecca Pollack
PURPOSE: Hematopoietic stem cell transplantation (HSCT) is a cure for sickle cell disease (SCD) but frequently results in permanent sterility. The complications associated with oocyte cryopreservation and risks of future pregnancy are increased in SCD patients. This case report discusses risk reduction strategies and includes a literature review of pregnancy after HSCT. CASE: A 23-year-old woman underwent ovarian stimulation for fertility preservation resulting in cancelation due to acute pain crisis...
August 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28780721/repeated-collection-of-conjoined-oocytes-from-a-patient-with-polycystic-ovary-syndrome-resulting-in-one-successful-live-birth-from-frozen-thawed-blastocyst-transfer-a-case-report
#9
Kohji Yano, Naoko Hashida, Toshiko Kubo, Ikuko Ohashi, Azusa Koizumi, Rumi Kageura, Kouichi Furutani, Chieko Yano
Few cases have been reported in which the aspiration of a single follicle led to the recovery of two conjoined oocytes surrounded by a single zona pellucida. This report describes a successful embryo transfer with subsequent live birth derived from conjoined oocytes, and a later pair of conjoined oocytes in the same patient. After oocyte retrieval from a patient with polycystic ovary syndrome, two pairs of conjoined oocytes were collected. One oocyte was fertilized using in vitro fertilization (IVF) and developed to the blastocyst stage...
August 5, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28776117/gnrh-antagonist-administered-twice-the-day-before-hcg-trigger-combined-with-a-step-down-protocol-may-prevent-ohss-in-ivf-icsi-antagonist-cycles-at-risk-for-ohss-without-affecting-the-reproductive-outcomes-a-prospective-randomized-control-trial
#10
Yannis Prapas, Konstantinos Ravanos, Stamatios Petousis, Yannis Panagiotidis, Achilleas Papatheodorou, Chrysoula Margioula-Siarkou, Assunta Iuliano, Giuseppe Gullo, Nikos Prapas
PURPOSE: The purpose this study is to investigate whether a double antagonist dose (0.25 mg/12 h) administered the day before hCG trigger is effective in preventing ovarian hyperstimulation syndrome (OHSS) in GnRH antagonist IVF/intracytoplasmic sperm injection (ICSI) cycles at risk for OHSS. METHODS: This is a prospective randomized control study, conducted from November 2012 to January 2016. A total of 194 patients undergoing a IVF/ICSI GnRH antagonist cycle that were at risk of OHSS and chose to proceed with embryo transfer and avoid cycle cancellation or embryo cryopreservation were allocated into two groups...
August 3, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28762037/genetic-polymorphism-of-growth-differentiation-factor-9-gdf9-gene-related-to-fecundity-in-two-egyptian-sheep-breeds
#11
Zaki A El Fiky, Gamal M Hassan, Mohamed I Nassar
PURPOSE: This study explores polymorphisms in the growth differentiation factor 9 (GDF9) gene (exon 1) with respect to fertility in Egyptian sheep. METHODS: Blood samples were collected, and genomic DNA was extracted from 24 Saidi and 13 Ossimi ewes. A 710 bp portion of the GDF9 gene, was amplified using specific primers, and the sequence was analyzed to clarify the phylogenetic relationship of Egyptian breed sheep. In addition, the PCR-RFLP method using Pst1 or Msp1 restriction enzymes was used to mask polymorphisms of partial exon 1 of GDF9 gene to establish molecular markers for twinning...
July 31, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28756497/natural-selection-between-day-3-and-day-5-6-pgd-embryos-in-couples-with-reciprocal-or-robertsonian-translocations
#12
Claire E Beyer, E Willats
PURPOSE: For translocation carriers, preimplantation genetic diagnosis (PGD) provides the opportunity to distinguish between normal/balanced and unbalanced embryos prior to implantation and, as such, increases the likelihood of a successful ongoing pregnancy. The data presented here compares autosomal reciprocal and Robertsonian translocation segregation patterns in day 3 versus day 5/6 IVF-PGD embryos to determine if there is a difference in the chromosome segregation patterns observed at these developmental time points...
July 29, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28756496/cryopreservation-of-human-ovarian-tissue-using-the-silver-closed-vitrification-system
#13
Zhun Xiao, Yaoyao Zhang, Wei Fan
PURPOSE: The aim of this study is to evaluate the feasibility of using a hand-made silver container for the cryopreservation of human ovarian cortex. METHODS: Human ovarian cortex tissues were vitrified using an open vitrification system (OVS) of needle immersed vitrification (NIV) and two closed vitrification systems (CVS) of a plastic vial (plastic CVS) and a silver container (silver CVS). Outcomes of vitrification were evaluated morphologically and histologically by in vitro culture and xenotransplantation...
July 29, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28755153/selection-of-mitochondria-in-female-germline-cells-is-balbiani-body-implicated-in-this-process
#14
REVIEW
Szczepan M Bilinski, Malgorzata Kloc, Waclaw Tworzydlo
Early oocytes of nearly all animal species contain a transient organelle assemblage termed the Balbiani body. Structure and composition of this assemblage may vary even between closely related species. Despite this variability, the Balbiani body always comprises of numerous tightly clustered mitochondria and accumulations of nuage material. It has been suggested that the Balbiani body is an evolutionarily ancestral structure, which plays a role in various processes such as the localization of organelles and macromolecules to the germ plasm, lipidogenesis, as well as the selection/elimination of dysfunctional mitochondria from female germline cells...
July 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28755152/assisted-reproduction-and-risk-of-preterm-birth-in-singletons-by-infertility-diagnoses-and-treatment-modalities-a-population-based-study
#15
Galit Levi Dunietz, Claudia Holzman, Yujia Zhang, Chenxi Li, David Todem, Sheree L Boulet, Patricia McKane, Dmitry M Kissin, Glenn Copeland, Dana Bernson, Michael P Diamond
PURPOSE: The purpose of this study is to examine the spectrum of infertility diagnoses and assisted reproductive technology (ART) treatments in relation to risk of preterm birth (PTB) in singletons. METHODS: Population-based assisted reproductive technology surveillance data for 2000-2010 were linked with birth certificates from three states: Florida, Massachusetts, and Michigan, resulting in a sample of 4,370,361 non-ART and 28,430 ART-related singletons. Logistic regression models with robust variance estimators were used to compare PTB risk among singletons conceived with and without ART, the former grouped by parental infertility diagnoses and treatment modalities...
July 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28755151/ivf-oocyte-retrieval-prospective-evaluation-of-the-type-of-anesthesia-on-live-birth-rate-pain-and-patient-satisfaction
#16
Lucie Rolland, Jeanne Perrin, Virginie Villes, Valérie Pellegrin, Léon Boubli, Blandine Courbiere
PURPOSE: Does the type of anesthesia (paracervical block (PCB) or general anesthesia (GA)) impact live birth rate, pain, and patient satisfaction? METHODS: A non-randomized prospective cohort study was conducted in women treated for IVF. Two groups of patients were prospectively included: the PCB group (n = 234) and the GA group (n = 247). The type of anesthesia was determined by the patients. The primary endpoint was cumulative live birth rate by OR. Secondary endpoints were self-assessment of the patients' peri-operative abdominal and vaginal pain vs the doctors' evaluations during PCB, post-operative abdominal and vaginal pain level, and patient satisfaction in both groups...
July 28, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28748493/cost-effectiveness-of-preimplantation-genetic-screening-for-women-older-than-37-undergoing-in-vitro-fertilization
#17
Stephen C Collins, Xiao Xu, Winifred Mak
PURPOSE: Adding preimplantation genetic screening to in vitro fertilization has been shown to increase live birth rate in women older than 37. However, preimplantation genetic screening is an expensive procedure. Information on the cost-effectiveness of preimplantation genetic screening can help inform clinical decision making. METHODS: We constructed a decision analytic model for a hypothetical fresh, autologous in vitro fertilization cycle (with versus without preimplantation genetic screening) for women older than age 37 who had a successful oocyte retrieval and development of at least one blastocyst...
July 27, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28733802/assisted-reproductive-technology-treatments-and-quality-of-life-a-longitudinal-study-among-subfertile-women-and-men
#18
Francesca Agostini, Fiorella Monti, Federica Andrei, Marcella Paterlini, Stefano Palomba, Giovanni Battista La Sala
PURPOSE: Quality of life (QoL) may represent a comprehensive indicator for the assessment of the psychological impact of complex clinical conditions, such as infertility. Infertile women have a worse QoL compared to both infertile men and non-infertile controls. However, the initial phases of infertility treatments have been frequently investigated using cross-sectional study designs. This prospective longitudinal study aimed at assessing the health-related QoL change across different phases of assisted reproductive technology (ART) treatments in subfertile women and men...
July 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28733801/effect-of-follicular-flushing-on-reproductive-outcomes-in-patients-with-poor-ovarian-response-undergoing-assisted-reproductive-technology
#19
Anna L M Souza, Marcos Sampaio, Graciele B Noronha, Ludiana G R Coster, Roberta S G de Oliveira, Selmo Geber
PURPOSE: The purpose of this study is to investigate the impact of follicular flushing on the number of oocytes retrieved, oocyte maturity, fertilization rate, embryo development, and pregnancy rate of poor ovarian responders (POR). METHODS: Retrospective study of 524 cycles of 384 patients with POR submitted to assisted reproductive technology (ART) and who had follicular flushing during oocyte retrieval was used in the study. We included patients with <5 oocytes at oocyte retrieval (POR group) and matching the Bologna criteria...
July 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28721540/temporal-expression-of-cumulus-cell-marker-genes-during-in-vitro-maturation-and-oocyte-developmental-competence
#20
Arindam Dhali, Pradeep Krishna Javvaji, Atul P Kolte, Joseph Rabinson Francis, Sudhir C Roy, Veerasamy Sejian
PURPOSE: Cumulus cells (CC) play important roles in oocyte development and cumulus expressed genes can be used as markers for oocyte quality. This study aimed to investigate temporal changes in the expression of cumulus marker genes during oocyte maturation as possible biomarkers of embryo developmental competence in ovine. METHODS: Gene expression was assessed in the CC of the BCB+ (developmentally competent) and BCB- (developmentally poor) oocytes at 0, 12, and 24 h of in vitro maturation (IVM)...
July 18, 2017: Journal of Assisted Reproduction and Genetics
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