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Journal of Assisted Reproduction and Genetics

Liying Ying, Xiaomin Chen, Lai Har Wu, Jing Shu, Xiangli Wu, Alice Yuen Loke
PURPOSE: Couples as dyads suffer from the diagnosis of infertility and related treatment. These couples commonly experience emotional and physical pain and tension in their marital lives. The purpose of this study is to report on the process of developing a potentially feasible and effective complex intervention for couples undergoing in vitro fertilization treatment in China. METHODS: The Medical Research Council (MRC) framework for developing and evaluating the complex intervention was adopted to guide the development of the Partnership and Coping Enhancement Programme (PCEP)...
October 15, 2016: Journal of Assisted Reproduction and Genetics
Konstantinos A Economou, Dimitra Christopikou, Erika Tsorva, Stephen Davies, Minas Mastrominas, Haris Cazlaris, Michael Koutsilieris, Panagoula Angelogianni, Dimitris Loutradis
PURPOSE: Artificial oocyte activation using calcium ionophores and enhancement of embryonic developmental potential by the granulocyte-macrophage colony-stimulating factor (GM-CSF) have already been reported. In this study, we evaluated the synergistic effect of these two methods on aged human unfertilized oocytes after intracytoplasmic sperm injection (ICSI). Then, we cultured the resulting embryos to the blastocyst stage and screened them for chromosomal abnormalities, to assess the safety of this protocol...
October 14, 2016: Journal of Assisted Reproduction and Genetics
Zuying Xu, Shinan Wu, Qiong Xing, Xi Wang, Huifen Xiang, Yuping Xu, Jing Wang, Xiaojin He, Binbin Wang, Yunxia Cao
PURPOSE: The study aims to investigate the genetic association between paired-box 2 gene (PAX2) and mullerian duct anomalies (MDA) in Chinese Han females. METHODS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify the genotypes of three tag single nucleotide polymorphisms (SNPs) in PAX2 in 362 MDA cases and 406 controls. RESULTS: We found that one tag SNP (rs12266644) of PAX2 was associated with susceptibility to MDA...
October 8, 2016: Journal of Assisted Reproduction and Genetics
Alexander M Quaas, Karl R Hansen
No abstract text is available yet for this article.
October 8, 2016: Journal of Assisted Reproduction and Genetics
Sherman Silber
After fresh or frozen ovary transplantation, FSH levels return to normal, and menstrual cycles resume by 150 days, coincident with anti-Müllerian hormone rising to higher than normal levels. AMH then returns to well below normal levels by 240 days, remaining as such for many years with nonetheless normal ovulation and fertility. To date, 20 babies have been born in our program from 11 fresh and 13 cryopreserved ovary transplant recipients with a live baby rate of over 70 % (11 babies from fresh and 9 from frozen)...
October 8, 2016: Journal of Assisted Reproduction and Genetics
Jigal Haas, Jim Meriano, Carl Laskin, Yaakov Bentov, Eran Barzilay, Robert F Casper, Ken Cadesky
PURPOSE: The aim of this study was to compare the pregnancy rates between good quality blastocysts vitrified on day 6 versus blastocysts vitrified on day 5 after fertilization. METHODS: This is a retrospective cohort study of 791 freeze-thaw cycles of blastocysts vitrified either on day 5 or on day 6 and transferred between January 2012 and October 2015. Five hundred and thirty-seven cycles included blastocysts vitrified on day 5, and 254 cycles included blastocysts vitrified on day 6...
October 6, 2016: Journal of Assisted Reproduction and Genetics
Lobke Bastings, Johan R Westphal, Catharina C M Beerendonk, Ruud L M Bekkers, Petra L M Zusterzeel, Jan C M Hendriks, Didi D M Braat, Ronald Peek
PURPOSE: Different protocols are being used worldwide for the cryopreservation of human ovarian tissue for fertility preservation purposes. The efficiency and efficacy of the majority of these protocols has not been extensively evaluated, possibly resulting in sub-optimally cryopreserved ovarian tissue. To address the impact of this issue, we assessed the effects of two clinically successful human ovarian tissue slow-freezing cryopreservation procedures on the quality of the cryopreserved tissue...
October 6, 2016: Journal of Assisted Reproduction and Genetics
Mario Vega, Sangita Jindal
No abstract text is available yet for this article.
October 4, 2016: Journal of Assisted Reproduction and Genetics
Liron Bar-El, Yael Kalma, Mira Malcov, Tamar Schwartz, Shaul Raviv, Tania Cohen, Hadar Amir, Yoni Cohen, Adi Reches, Ami Amit, Dalit Ben-Yosef
PURPOSE: The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. METHODS: The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos)...
October 1, 2016: Journal of Assisted Reproduction and Genetics
Mireia Solé, Joan Blanco, Oliver Valero, Laia Vergés, Francesca Vidal, Zaida Sarrate
PURPOSE: The study aims to determine whether there is an altered bivalent positioning in metaphase I human spermatocytes from Robertsonian translocation carriers. METHODS: Metaphase I human spermatocytes from three 45,XY,der(13;14)(q10;q10) individuals and a 45,XY,der(14;15)(q10;q10) individual were analyzed. Proximity relationships of bivalents were established by analyzing meiotic preparations combining Leishman staining and multiplex-FISH procedures. Poisson regression model was used to determine proximity frequencies between bivalents and to assess associations with chromosome size, gene density, acrocentric morphology, and chromosomes with heterochromatic blocks...
September 21, 2016: Journal of Assisted Reproduction and Genetics
Ettore Caroppo, Elisabetta M Colpi, Giacomo Gazzano, Liborio Vaccalluzzo, Fabrizio I Scroppo, Giuseppe D'Amato, Giovanni M Colpi
PURPOSE: The present study sought to determine the diagnostic accuracy of FSH level, testicular volume, and testicular histology in predicting the successful sperm retrieval (SSR) in a large cohort of patients with non-obstructive azoospermia undergoing conventional testicular sperm extraction (TESE). METHODS: We retrospectively evaluated 356 patients with non-obstructive azoospermia between June 2004 and July 2009. Binary logistic regression was used to evaluate the diagnostic accuracy of our predicting model, identifying sperm retrieval rate as binary dependent variable...
September 21, 2016: Journal of Assisted Reproduction and Genetics
John Huntriss, Jianping Lu, Karen Hemmings, Rosemary Bayne, Richard Anderson, Anthony Rutherford, Adam Balen, Kay Elder, Helen M Picton
PURPOSE: Gametocyte-specific factor 1 has been shown in other species to be required for the silencing of retrotransposons via the Piwi-interacting RNA (piRNA) pathway. In this study, we aimed to isolate and assess expression of transcripts of the gametocyte-specific factor 1 (GTSF1) gene in the human female germline and in preimplantation embryos. METHODS: Complementary DNA (cDNA) libraries from human fetal ovaries and testes, human oocytes and preimplantation embryos and ovarian follicles isolated from an adult ovarian cortex biopsy were used to as templates for PCR, cloning and sequencing, and real time PCR experiments of GTSF1 expression...
September 19, 2016: Journal of Assisted Reproduction and Genetics
Nae Tanpradit, Kaywalee Chatdarong, Pierre Comizzoli
PURPOSE: Temporary and reversible downregulation of metabolism may improve the survival of tissues exposed to non-physiological conditions during transport, in vitro culture, and cryopreservation. The objectives of the study were to (1) optimize the concentration and duration of carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP-a mitochondrial uncoupling agent) exposures for biopsies of domestic cat ovarian tissue and (2) examine the effects of FCCP pre-exposures on follicle integrity after tissue culture and/or cryopreservation...
September 17, 2016: Journal of Assisted Reproduction and Genetics
Yamato Mizobe, Naoto Oya, Reiko Iwakiri, Naomi Yoshida, Yumi Sato, Nanase Onoue, Kazuchika Miyoshi, Makoto Tokunaga, Yuji Ezono
PURPOSE: In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment...
September 17, 2016: Journal of Assisted Reproduction and Genetics
C E Dunlop, B M Brady, M McLaughlin, E E Telfer, J White, F Cowie, S Zahra, W H B Wallace, R A Anderson
With the improvement of long-term cancer survival rates, growing numbers of female survivors are suffering from treatment-related premature ovarian insufficiency (POI). Although pre-treatment embryo and oocyte storage are effective fertility preservation strategies, they are not possible for pre-pubertal girls or women who cannot delay treatment. In these cases, the only available treatment option is ovarian cortex cryopreservation and subsequent re-implantation. A 32-year-old woman had ovarian cortex cryopreserved 10 years previously before commencing high-dose chemotherapy and undergoing a haematopoietic stem cell transplant for recurrent adult Wilms tumour, which resulted in POI...
September 17, 2016: Journal of Assisted Reproduction and Genetics
Raoul Orvieto, Norbert Gleicher
No abstract text is available yet for this article.
September 15, 2016: Journal of Assisted Reproduction and Genetics
Jing Xu, Fuhua Xu, John H Letaw, Byung S Park, Robert P Searles, Betsy M Ferguson
PURPOSE: The main goals of this study were to investigate the expression of anti-Müllerian hormone (AMH) and its receptor (AMHR2) during follicular development in primates, and to evaluate the potential of AMH as a biomarker for follicle growth and oocyte maturation in vitro. METHODS: The mRNA and protein expression of AMH and AMHR2 were determined using isolated follicles and ovarian sections from rhesus macaques (n = 4) by real-time PCR and immunohistochemistry, respectively...
September 15, 2016: Journal of Assisted Reproduction and Genetics
Natalia Basile, Juan A Garcia-Velasco
The recent development of vitrification technologies and the good outcomes obtained in assisted reproduction technologies have supported new indications for freezing and segmentation of treatment. Beyond OHSS prevention and avoidance of embryo transfers in the setting of an adverse endocrinological profile or endometrial cavity, we have witnessed a trend to shift fresh embryo transfers to frozen embryo transfers in many programs. We critically review the available evidence and suggest that freeze-all is not "for all," but should be individualized...
September 14, 2016: Journal of Assisted Reproduction and Genetics
Larissa Berloffa Belardin, Paula Toni Del Giudice, Mariana Camargo, Paula Intasqui, Mariana Pereira Antoniassi, Ricardo Pimenta Bertolla, Agnaldo Pereira Cedenho
PURPOSE: To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5(th) centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis...
September 14, 2016: Journal of Assisted Reproduction and Genetics
R H Goldman, D J Kaser, S A Missmer, S S Srouji, L V Farland, C Racowsky
PURPOSE: Many practices are moving away from cleavage-stage transfer in favor of blastocyst transfer. The purpose of this study is to evaluate how the overall live birth rate for fresh IVF cycles may increase by optimizing the day of transfer for each patient. METHODS: This is a retrospective cohort study of 1225 first fresh autologous IVF cycles performed between May 2012 and November 2013. Stepwise logistic regression was used to determine characteristics associated with live birth following cleavage-stage versus blastocyst transfer...
September 10, 2016: Journal of Assisted Reproduction and Genetics
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