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Microscopy Research and Technique

Syed Nasar Shah, Mushtaq Ahmad, Muhammad Zafar, Abdul Razzaq, Khafsa Malik, Neelam Rashid, Fazal Ullah, Majid Iqbal, Wajid Zaman
For the robust identification of taxonomically complex fern family like Athyriaceae, light and scanning electron microscopy is significance implications. This article present first microscopic investigation of foliar micromorphology of 3 genera and 10 species belonging to Athyriaceae namely, Athyrium, Deparia, and Diplazium were collected from different localities in Malakand Division, Northern Pakistan. In present study we compare foliar micromorphology of all 10 species using standard protocols of light microcopy (LM) and scanning electron microscopy...
July 10, 2018: Microscopy Research and Technique
Eun-Kyung Choi, Joon Hyung Sohn, Sung Sik Han, Dong Heui Kim
In teleost, the structural characteristics of fertilized egg and egg envelope are very important for classification of genus or species. The structures of fertilized egg and egg envelope from Corydoras adolfoi and Corydoras sterbai, Callichthyidae, Siluriformes in teleost were examined by scanning and transmission electron microscopes to confirm whether these morphological structures have specificities of species and family or not. The fertilized eggs of C. adolfoi and C. sterbai were non-transparent, spherical, demersal, and strong adhesive...
June 20, 2018: Microscopy Research and Technique
Jéssika Mayhara Pereira Morais, Keli Regina Victorino, Wilfredo Gustavo Escalante-Otárola, Keren Cristina Fagundes Jordão-Basso, Regina Guenka Palma-Dibb, Milton Carlos Kuga
The aim of the study was to evaluate the effects when acid etching on the dentin surface was immediately performed (I) or 7 days (D) after calcium silicate-based sealer (MTA Fillapex) removal, using 95% ethanol (E) or xylol (X). First study, 60 bovine incisor dentin specimens were impregnated with sealer and divided into six groups (n = 10): (EI), E + I; (XI), X + I; (ED), E + D; (XD), X + D, (UN), untreated and (MR), mechanical removal of sealer. Scanning electron microscopy (SEM) images (500×) were obtained from each specimen and scores assessed the sealer residues persistence...
June 15, 2018: Microscopy Research and Technique
Abtesam Aljdaimi, Hugh Devlin, Mark Dickinson, Abdullatif Alfutimie
The aim was to investigate the effect of the Er-YAG laser radiation on morphology and chemical composition of enamel, dentin, and bone. The specimens of the three groups were irradiated with a very long pulse mode (VLP) of 2.94 µm Er-YAG laser with 100 mJ pulse energy and energy density of 8.42 J/ cm2 for 30 s, at a repetition rate of 15 Hz. The organic and inorganic content of the samples were investigated by Fourier Transforms Infrared spectroscopy (FTIR). The morphological characteristics were investigated with scanning electron microscopy (SEM) and elemental analysis (calcium and phosphorus) with energy-dispersive X-ray spectroscopy (EDX)...
May 21, 2018: Microscopy Research and Technique
Seulgi Noh, Hyosun Choi, Ji Soo Kim, Il-Hwan Kim, Ji Young Mun
There is a global trend of increase in the demand for three-dimensional electron microscopy with high resolution. The ultrastructural change and related functional studies are necessary to investigate biological phenomena. In this study, currently available 3D reconstruction techniques of electron microscopes (serial block-face scanning electron microscopy and focused ion beam-scanning electron microscopy) were used to investigate hyperpigmentary disorders in human skin. In the basal layer of the epidermis in the human skin, there are melanocytes that produce melanin and keratinocytes that act as a barrier against environmental damage...
May 18, 2018: Microscopy Research and Technique
Hyun-Wook Kim, Seunghak Oh, Seung Hwan Lee, Sanghoon Lee, Ji-Eun Na, Kea Joo Lee, Im Joo Rhyu
Experience-dependent synapse remodeling is associated with information storage in the nervous system. Neuronal synapses show alteration in various neurological and cognitive disorders in their structure and function. At the ultrastructural level, parallel fiber boutons contacting multiple spines of Purkinje cells in the cerebellar cortex are commonly observed in physiologically enriched animals as well as pathological ataxic mutants. However, the dendritic origin of those spines on parallel fiber multiple-synapse boutons (MSBs) has been poorly understood...
May 18, 2018: Microscopy Research and Technique
Carolina Fabiana Centenaro, Fabiana Soares Grecca, Patrícia Maria Poli Kopper, Rodrigo Ricci Vivan, Jefferson Ricardo Pereira, Ricardo Abreu da Rosa, Paula Barcellos da Silva, Marcus Vinícius Reis Só
The purpose of this study was to quantify the presence of nickel (Ni) and titanium (Ti) on dentin walls of prepared root canals using Endosequence (ES) and Wave One (WO) systems, the deformation, and fracture of these instruments. Thirty extracted human premolar teeth were selected and prepared with WO, ES, and manually (control group-CG). Each instrument was used in four root canals. The root canals were irrigated with 2.5% sodium hypochlorite solution. After preparation, roots were sectioned longitudinally and the apical third was analyzed by scanning electron microscopy with X-ray microanalysis (SEM-EDS)...
May 13, 2018: Microscopy Research and Technique
D Suhard, C Tessier, L Manens, F Rebière, K Tack, M Agarande, Y Guéguen
Localization of uranium within cells is mandatory for the comprehension of its cellular mechanism of toxicity. Secondary Ion Mass Spectrometry (SIMS) has recently shown its interest to detect and localize uranium at very low levels within the cells. This technique requires a specific sample preparation similar to the one used for Transmission Electronic Microscopy, achieved by implementing different chemical treatments to preserve as much as possible the living configuration uranium distribution into the observed sample...
May 8, 2018: Microscopy Research and Technique
N Pornsuwancharoen, P Youplao, I S Amiri, M S Aziz, Q L Tran, J Ali, P Yupapin, K T V Grattan
A conventional Michelson interferometer is modified and used to form the various types of interferometers. The basic system consists of a conventional Michelson interferometer with silicon-graphene-gold embedded between layers on the ports. When light from the monochromatic source is input into the system via the input port (silicon waveguide), the change in optical path difference (OPD) of light traveling in the stacked layers introduces the change in the optical phase, which affects to the electron mean free path within the gold layer, induces the change in the overall electron mobility can be seen by the interferometer output visibility...
May 8, 2018: Microscopy Research and Technique
María de Los Angeles Moyaho-Bernal, Rosalía Contreras-Bulnes, Laura Emma Rodríguez-Vilchis, Efraín Rubio-Rosas
Innovators conditioning protocols are emerged in permanent dentin, however for deciduous dentin the information is limited; the aim of this study was to evaluate in vitro diameter of deciduous and permanent dentinal tubules after several conditioning protocols. Eighty dentin samples were distributed in sixteen groups (n = 5 p/g) and dentin surface was conditioned as follow: G1D/G1P acid etching; G2D/G2P, self-etch adhesive; G3D/G3P, G4D/G4P, Er: YAG laser irradiation at 200 mJ-25.5 J/cm2 and 300 mJ-38.2 J/cm2 , at 10 Hz under water spray respectively; G5D/G5P, G6D/G6P, G7D/G7P, and G8D/G8P were irradiated under the same energy densities followed phosphoric acid or self-etch adhesive conditioning...
May 8, 2018: Microscopy Research and Technique
Mohamed A M Alsafy, Naglaa F Bassuoni, Basma G Hanafy
Bagrus Bayad (Forskal, 1775) is a bottom feeder fish feeds on different food items. Its oral cavity anatomy has many types of teeth adapted to the capacity and varieties of feeding. The aim of the present study was to use the gross anatomy and scanning electron microscopy to identify the anatomical characteristic, especially of the teeth and taste buds distributed on the roof and floor of the oropharyngeal cavity. The results clarified that Bagrus Bayad has two types of teeth; curved pointed and blunt teeth of different sizes...
May 8, 2018: Microscopy Research and Technique
Serdar Baglar, Umit Erdem, Mustafa Dogan, Mustafa Turkoz
In this in-vitro study, the effectiveness of experimental pure nano-hydroxyapatite (nHAP) and 1%, 2%, and 3% F¯ doped nano-HAp on dentine tubule occlusion was investigated. And also, the cytotoxicity of materials used in the experiment was evaluated. Nano-HAp types were synthesized by the precipitation method. Forty dentin specimens were randomly divided into five groups of; 1-no treatment (control), 2-specimens treated with 10% pure nano-HAp and 3, 4, 5 specimens treated with 1%, 2%, and 3% F- doped 10% nano-HAp, respectively...
April 29, 2018: Microscopy Research and Technique
J M Budel, V Raman, L M Monteiro, V P Almeida, V B Bobek, G Heiden, I J M Takeda, I A Khan
We report for the first time the presence of cluster crystals of calcium oxalate within the glandular trichomes and oil bodies in the mesophyll for Baccharis species. Moreover, the comparative leaf anatomy and micro-morphology of six species of Baccharis, namely B. illinita, B. microdonta, B. pauciflosculosa, B. punctulata, B. reticularioides, and B. sphenophylla is investigated by light and scanning electron microscopy. The studied species exhibited differences in their leaf anatomical features such as the morphology of the cuticle, type and occurrence of the stomata, presence or absence of glandular trichomes, shape of the flagelliform trichomes, and the arrangement of the mesophyll tissues...
April 27, 2018: Microscopy Research and Technique
Monika Ostapiuk, Bożena Tarczydło, Barbara Surowska, Mirosław Orłowski, Barbara Tymczyna, Teresa Bachanek, Anna Rzepecka, Anna Mróz
PURPOSE: In this study the comparative results obtained from examinations of two different restorative composites with different cavities filling method are presented. Micro-CT was used for the evaluation of the marginal integrity and quality of composite fillings in connection with microstructural investigations made by both optical and scanning electron microscopy. MATERIALS AND METHODS: The examinations of dental tooth filings in bulk-fill technique were based on micro-CT and microscopy observations...
April 24, 2018: Microscopy Research and Technique
Luan Rissatto Sierra, Gabriel Fávaro, Bruno Rubin Cerri, Lara Caetano Rocha, Sonia Regina de Yokomizo de Almeida, Ii-Sei Watanabe, Adriano Polican Ciena
The study aims to describe the tissue plasticity of MTJ through the morphological analysis of MTJ soleus in ovariectomized aged female Wistar rats submitted to aquatic training. Forty aged Wistar rats, 1 year and 2 months of age, were divided into four groups: sedentary (S), trained (T), ovariectomized (O), and trained/ovariectomized (OT). Employing the transmission electron microscopy, the ultrastructural and morphometric elements were revealed. In the S group, changes in morphological characteristics as a consequence of the aging process were seen, demonstrated by the conical shape of the muscle cell extremity, a large area with collagen deposit, and misalignment of sarcomeres in series...
April 24, 2018: Microscopy Research and Technique
Dong Won Lee, Dae Yeon Kim, Ji Hyuck Hong, Soo Hong Seo, Young Chul Kye, Hyo Hyun Ahn
Skin biopsy for AK diagnosis is usually performed on only a limited part of the whole lesion. Therefore, a clinical diagnosis is important. According to a study, there is no significant correlation between histopathological and clinical classification system. We examined the correlation between microscopic information and dermoscopic findings to deduce if dermoscopic information reflects histopathologic grade severity. Forty seven patients with histologically confirmed AK were enrolled and positive ratio of red pseudonetwork, rosette, red background and targetoid signs, white-to-yellow scale, white structureless area, and pigmentation from dermoscopic findings were investigated...
April 20, 2018: Microscopy Research and Technique
Visittapong Yordsri, Chanchana Thanachayanont, Chabaiporn Junin, Masahiro Kawasaki, Shunsuke Asahina, Tetsuo Oikawa, Atsushi Saiki, Tadashi Nobuchi, Makoto Shiojiri
Green culms of Bambusa multiplex and the bamboo charcoal carbonized from the green culms at 700°C have been studied by means of X-ray diffraction, X-ray fluorescent element analysis, analytical scanning electron microscopy, and analytical scanning transmission electron microscopy (STEM), aiming at industrial applications as raw materials for functional devices and substances. It is revealed that the green culms and the charcoal contain a significant amount of Si, in particular, ∼18 wt % in the skin. The green culms comprise amorphous and crystalline celluloses...
April 20, 2018: Microscopy Research and Technique
Ki Woo Kim
External and internal features of Elaeagnus umbellata leaves were investigated by optical and electron microscopy. The adaxial (upper) and abaxial (lower) leaf surfaces appeared green and silver in color, respectively. There were peltate trichomes on both the adaxial and abaxial leaf surfaces. The peltate trichomes were 200-300 μm in diameter and shield or umbrella-shaped. They had a central dome and 20-30 radiating rays that were fused to form a circular cap in the center and tapered at the end. The density of peltate trichomes was apparently higher on the abaxial leaf surface than on the adaxial leaf surface...
April 20, 2018: Microscopy Research and Technique
Shao-Pu Tsai, Shih-Ning Tsai, Yu-Ting Tsai, Yu-Wen Chen, Po-Yen Tung, Jer-Ren Yang, Hsueh-Ren Chen, Chih-Yuan Chen, Yuan-Tsung Wang, Ching-Yuan Huang
Convergent beam electron diffraction (CBED) in transmission electron microscopy (TEM) was applied to determine local carbon concentrations in low-carbon transformation-induced plasticity (TRIP) steels. High-order Laue-zone (HOLZ) lines were experimentally obtained for comparison with simulation results. A new procedure for calculating carbon content is thus proposed. Retained austenite (RA) is classified into three types by morphology; the relationship between the carbon content and the corresponding RA morphology is discussed based on CBED results...
April 20, 2018: Microscopy Research and Technique
Chunhong Li, Hui Ruan, Dengming Chen, Kejian Li, Donglin Guo, Bin Shao
A Ni-based alloy was heat treated by changing the temperature and ambient atmosphere of the heat treatment. Morphology, crystal structure, and physical performance of the Ni-based alloy were characterized via SEM, XRD, TEM, and PPMS. Results show that due to the heat treatment process, the grain growth of the Ni-based alloy and the removal of impurities and defects are promoted. Both the orientation and stress caused by rolling are reduced. The permeability and saturation magnetization of the alloy are improved...
April 20, 2018: Microscopy Research and Technique
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