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Microscopy Research and Technique

Bushra Mughal, Muhammad Sharif, Nazeer Muhammad, Tanzila Saba
Early screening of skeptical masses or breast carcinomas in mammograms is supposed to decline the mortality rate among women. This amount can be decreased more on development of the computer-aided diagnosis with reduction of false suppositions in medical informatics. Our aim is to provide a robust tumor detection system for accurate classification of breast masses using normal, abnormal, benign, or malignant classes. The breast carcinomas are classified on the basis of observed classes. This is highly dependent on feature extraction process...
November 16, 2017: Microscopy Research and Technique
Raimundo Nonato Silva Gomes, Tanmoy T Bhattacharjee, Luis Felipe C S Carvalho, Luís Eduardo Silva Soares
Medicaments essential for alleviation of diseases may sometime adversely affect dental health by eroding the enamel, owing to their acidic nature. It is therefore highly desirable to be able to detect these effects quickly and reliably. In this study, we evaluated the erosive capacity of four most commonly prescribed respiratory disease syrup medicaments on enamel using micro-energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR)...
November 14, 2017: Microscopy Research and Technique
Vladimir L Kolossov, Mayandi Sivaguru, Joseph Huff, Katherine Luby, Kaviamuthan Kanakaraju, H Rex Gaskins
Mitochondrial morphology is regulated by continuous fusion-and-fission events that are essential for maintaining normal function. Despite the prominence of mitochondrial function in energy generation and cell signaling, understanding of processes of fusion and fission dynamics has been hampered by the lack of high-resolution optical systems that accommodate live-cell imaging. We have examined different confocal modalities in terms of resolution and signal-to-noise ratio (SNR) in a point scanning confocal microscope with Airyscan super-resolution (AS-SR)...
November 13, 2017: Microscopy Research and Technique
Nidambur Vasudev Ballal, Özgür İlke Ulusoy, Shubha Chhaparwal, Kishore Ginjupalli
To compare the effects of different chelating agents on the push-out bond strength of calcium silicate-based cements to the simulated root-end cavities. Root-end cavities were prepared on the roots of fifty extracted maxillary anterior teeth. The specimens were then randomly divided into 5 groups (n = 10) based on the final irrigation regimen: Group 1: 17% EDTA, Group 2: 7% maleic acid, Group 3: QMix, Group 4: 2.25% peracetic acid (PAA), Group 5: 0.9% saline. Then, the samples from each group were subdivided into two groups (n = 5) based on the apical filling material...
November 11, 2017: Microscopy Research and Technique
Junbin Dong, Haiquan Bao, Lai Mang
Rhinoestrus sp. (Diptera: Oestridae) is an economically important parasite that can cause severe nasal myiasis in equids and can also affect humans. The ultrastructure of all Rhinoestrus sp. larval instars from Mongolian horse was examined by light and scanning electron microscopy to characterize the features of Rhinoestrus. The structure of the anterior region, posterior region, and the spines of the third segment was analyzed for 10 specimens in each larval stage. Additionally, 34 third-instar (L3) larvae of Rhinoestrus sp...
November 8, 2017: Microscopy Research and Technique
Hoon-Seok Jang, Mannan Saeed Muhammad, Tae-Sun Choi
In regard to Shape from Focus, one critical factor impacting system application is mechanical vibration of the translational stage causing jitter noise along the optical axis. This noise is not detectable by simply observing the image. However, when focus measures are applied, inaccuracies in the depth occur. In this article, jitter noise and focus curves are modeled by Gaussian distribution and quadratic function, respectively. Then Kalman filter is designed and applied to eliminate this noise in the focus curves, as a post-processing step after the focus measure application...
November 8, 2017: Microscopy Research and Technique
Adrieli Burey, Paulo José Dos Reis, Bruno Luiz Santana Vicentin, Cássia Cilene Dezan Garbelini, Márcio Grama Hoeppner, Carlos Roberto Appoloni
This research aims to probe the porosity profile and polymerization shrinkage of two different dual cure resin cements with different dentin bonding systems. The self-adhesive resin cement RelyX U200 (named RU) and the conventional Allcem Core (named AC) were analyzed by x-ray microtomography (μCT) and Scanning Electron Microscopy (SEM). Each cement was divided into two groups (n = 5): dual-cured (RUD and ACD) and self-cured (RUC and ACC). μCT demonstrated that the method of polymerization does not influence the porosity profile but the polymerization shrinkage...
October 30, 2017: Microscopy Research and Technique
Rayana Longo Bighetti Trevisan, Renata Siqueira Scatolin, Larissa Moreira Spinola de Castro Raucci, Walter Raucci Neto, Izabel Cristina Froner
The aim of this study was to evaluate the effect of 24% ethylenediaminetetraacetic acid (EDTA) gel and 2% chlorhexidine gel (CHX) in dentin permeability and smear layer removal from root canals instrumented with NiTi rotary system using histochemical staining and scanning electron microscopy (SEM) analysis. Overall, 43 premolars were classified into two experimental groups, EDTA (n = 20) and CHX (n = 20), and a negative control (NC) (n = 3). All specimens were instrumented and the irrigant solutions were used after each file change...
October 30, 2017: Microscopy Research and Technique
Camila Santos de Mattos Brito, Kássia Regina Simões Meira, Frederico Barbosa de Sousa
This study aimed to test the hypotheses that (i) a parameter related to permeability, αd (ratio of squared water volume by the nonmineral volume) is, among all major component volumes (mineral, water, and organic volumes) the best predictor of quinoline infiltration in natural enamel caries (NEC), and (ii) the pore volume fraction infiltrated by quinoline (Vqui ) in NEC is much lower than previous estimates that neglected water and organic enamel volumes. Mineral and nonmineral volumes and αd were measured at 341 histological points (from 20 approximal NEC lesions), and transport of quinoline was tracked by orientation-independent polarizing microscopy...
October 30, 2017: Microscopy Research and Technique
Alexandre Fioravante de Siqueira, Flávio Camargo Cabrera, Wagner Massayuki Nakasuga, Aylton Pagamisse, Aldo Eloizo Job
Image segmentation, the process of separating the elements within a picture, is frequently used for obtaining information from photomicrographs. Segmentation methods should be used with reservations, since incorrect results can mislead when interpreting regions of interest (ROI). This decreases the success rate of extra procedures. Multi-Level Starlet Segmentation (MLSS) and Multi-Level Starlet Optimal Segmentation (MLSOS) were developed to be an alternative for general segmentation tools. These methods gave rise to Jansen-MIDAS, an open-source software...
October 20, 2017: Microscopy Research and Technique
Manuela Cesaretti, Nicolas Poté, Federica Dondero, François Cauchy, Anne Sophie Schneck, Olivier Soubrane, Valerie Paradis, Alberto Diaspro, Andrea Antonini
Light microscopy is an essential tool in histological examination of tissue samples. However, the required equipment for a correct and rapid diagnosis is sometimes unavailable. Smartphones and mobile phone networks are widespread, and could be used for diagnostic imaging and telemedicine. Macrovesicular steatosis (MS) is a major risk factor for liver graft failure, and is only assessable by microscopic examination of a frozen tissue section. The aim of this study was to compare the microscopic assessment of MS in liver allograft biopsies by a smartphone with eyepiece adaptor (BLIPS device) to standard light microscopy...
October 17, 2017: Microscopy Research and Technique
Raees Khan, Sheikh Zain Ul Abidin, Mushtaq Ahmad, Muhammad Zafar, Jie Liu, Hafiza Amina
The present study is intended to assess gymnosperms pollen flora of Pakistan using Light Microscope (LM) and Scanning Electron Microscopy (SEM) for its taxonomic significance in identification of gymnosperms. Pollens of 35 gymnosperm species (12 genera and five families) were collected from its various distributional sites of gymnosperms in Pakistan. LM and SEM were used to investigate different palyno-morphological characteristics. Five pollen types (i.e., Inaperturate, Monolete, Monoporate, Vesiculate-bisaccate and Polyplicate) were observed...
October 17, 2017: Microscopy Research and Technique
K A El-Farahaty, M A El-Bakary, N M El-Sayed
Samples of polyglycolic acid PGA/polycaprolactone PCL copolymer absorbable suture materials, which is called Poliglecaprone PGC copolymer suture, were thermally treated by two different processes. The first method is the direct heat exposure, from 20 to 38 °C, in the surrounding medium. Which is an environmental study. The other method is the annealing process at temperatures ranged from 65, 80, 100, 120, and 140 °C, for two different duration periods 60 and 120 min. This is an extensive technical study...
October 13, 2017: Microscopy Research and Technique
G B Morais, D A Viana, J M Verdugo, M G Roselló, J O Porcel, D D Rocha, F A F Xavier Júnior, K D S M Barbosa, F M O Silva, G A C Brito, C M S Sampaio, J S A M Evangelista
Renal fibrosis is characterized by glomerulosclerosis and tubulointerstitial fibrosis and its pathogenesis is associated with the activity of mesenchymal cells (fibroblasts), being essentially characterized by a process of excessive accumulation resulting from the deposition of extracellular matrix components. The aim of this study was to characterize the morphological presentation of chronic and fibrotic lesions in the glomerular, tubular, interstitial, and vascular compartments in feline CKD, as well as the possible participation of myofibroblasts in renal fibrotic processes in this species...
October 12, 2017: Microscopy Research and Technique
Jinyun Liu, Yingmin Qu, Guoliang Wang, Xinyue Wang, Wenxiao Zhang, Jingmei Li, Zuobin Wang, Dayou Li, Jinlan Jiang
This article studies the morphological and mechanical features of multinuclear and mononuclear SW480 colon cancer cells by atomic force microscopy to understand their drug-resistance. The SW480 cells were incubated with the fullerenol concentrations of 1 mg/ml and 2 mg/ml. Morphological and mechanical features including the height, length, width, roughness, adhesion force and Young's modulus of three multinuclear cell groups and three mononuclear cell groups were imaged and analyzed. It was observed that the features of multinuclear cancer cells and mononuclear cancer cells were significantly different after the treatment with fullerenol...
October 9, 2017: Microscopy Research and Technique
Bojana D Ramic, Milan R Drobac, Igor L Stojanac, Milica T Premovic, Ljubomir M Petrovic
The aim of this study was to analyse the interfacial micromorphology of total-etch adhesives and dentin structures different locations by using SEM. Standardized cylindrical cavities (3mm in diameter, 2mm deep) with all margins in dentin were prepared on occlusal and buccal surfaces of twenty extracted human third molars. A total-etch dentin adhesive system and a light-cure flowable composite (Filtek Ultimate Flowable, 3M ESPE, St. Paul, MN, USA) were used in this study. Micro-morphological SEM analysis of the marginal seal of the original tooth specimens was performed using high magnification of up to 1000×...
October 7, 2017: Microscopy Research and Technique
Mohammadreza Momenzadeh, Alireza Vard, Ardeshir Talebi, Alireza Mehri Dehnavi, Hossein Rabbani
Vulvovaginal candidiasis (VVC) is a common gynecologic infection and it occurs when there is overgrowth of the yeast called Candida. VVC diagnosis is usually done by observing a Pap smear sample under a microscope and searching for the conidium and mycelium components of Candida. This manual method is time consuming, subjective and tedious. Any diagnosis tools that detect VVC, semi- or full-automatically, can be very helpful to pathologists. This article presents a computer aided diagnosis (CAD) software to improve human diagnosis of VVC from Pap smear samples...
October 7, 2017: Microscopy Research and Technique
Maria Sumarokova, Jagoba Iturri, José L Toca-Herrera
Fibronectin is an extracellular matrix protein that is involved in cell adhesion, growth, migration, differentiation, and wound healing. Fibronectin coatings are currently used in many laboratories for biomedical and biotechnology purposes. In this study we have investigated the adhesion and mechanical properties of fibronectin coatings. The coatings were also used to study the role of the residence time and the influence of the loading rate in nonspecific interactions. The results showed that the adhesion force between silica and fibronectin increased with loading rate delivering similar values for residence times of 1 and 2 s...
October 7, 2017: Microscopy Research and Technique
Maryam H Shirzeily, Neda Khanlarkhani, Fardin Amidi, Farshad H Shirzeily, Fereydoon S Aval, Aligholi Sobhani
Nowadays, infertility is no longer considered as an unsolvable disorder due to progresses in germ cells derived from stem lineage with diverse origins. Technical and ethical challenges push researchers to investigate various tissue sources to approach more efficient gametes. The purpose of the current study is to investigate the efficacy of a combined medium, retinoic acid (RA) together with Bone Morphogenic Protein-4 (BMP4), on differentiation of Bone Marrow Mesenchymal Stem Cells (BMMSCs) and adipose-derived mesenchymal stem cells (ADMSCs) into germ cells...
September 18, 2017: Microscopy Research and Technique
Patricia Das Neves Borges, Tonia L Vincent, Massimo Marenzana
Murine models of osteoarthritis (OA) are increasingly important for understating pathogenesis and for testing new therapeutic approaches. Their translational potential is, however, limited by the reduced size of mouse limbs which requires a much higher resolution to evaluate their articular cartilage compared to clinical imaging tools. In experimental models, this tissue has been predominantly assessed by time-consuming histopathology using standardized semi-quantitative scoring systems. This study aimed to develop a novel imaging method for 3-dimensional (3D) histology of mouse articular cartilage, using a robotic system-termed here "3D histocutter"-which automatically sections tissue samples and serially acquires fluorescence microscopy images of each section...
December 2017: Microscopy Research and Technique
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