journal
MENU ▼
Read by QxMD icon Read
search

Microscopy Research and Technique

journal
https://www.readbyqxmd.com/read/28816411/a-stereovision-model-applied-in-bio-micromanipulation-system-based-on-stereo-light-microscope
#1
Yuezong Wang
A bio-micromanipulation system is designed for manipulating micro-objects with a length scale of tens or hundreds of microns based on stereo light microscope. The world coordinate reconstruction of points on the surface of micro-objects is an important goal for the micromanipulation. Traditional pinhole camera model is applied widely in macrocomputer vision. However, this model will output bad data with remarkable error if it is directly used to reconstruct three-dimensional world coordinates for stereo light microscope...
August 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28816410/new-insights-into-the-cellular-makeup-and-progenitor-potential-of-palatal-connective-tissues
#2
Emoke Pall, Mihai Cenariu, Adrian Kasaj, Adrian Florea, Andrada Soancă, Alexandra Roman, Carmen Georgiu
The present study investigated the regenerative potential of connective tissues harvested from two palatal areas widely used as donor sites for muco-gingival surgical approaches. Connective tissue grafts (CTGs) were obtained by de-epithelialisation of a free gingival graft (deCTG) and by a split flap approach from a previous donor site (reCTG). Two types of mesenchymal stem cell (MSCs) were isolated and were named de-epithelialised MSCs (deMSCs) and re-entry MSCs (reMSCs). The cells were characterised and cellular functionality was investigated...
August 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28816383/the-structure-and-ultrastructure-of-the-egg-capsules-of-stoneflies-of-the-genus-isoperla-insecta-plecoptera-perlodidae
#3
Anna Michalik, Marko Miliša, Katarzyna Michalik, Elżbieta Rościszewska
The egg capsules of five systellognathan stoneflies species representing the genus Isoperla (Plecoptera, Perlodidae) have been investigated using light and electron microscopes (SEM and TEM). We consider the structural modifications of egg coverings (egg capsules, eggshells) like: a shape of a capsule, presence of structures fixing an egg to the substratum under water, intrachorionic aeropylar system facilitating gas exchange as a factor adapting an egg/embryo to the environment. The structures protecting eggs against desiccation during a female flight before oviposition into water as well as against the dangers of external mechanical injury caused by turbulences in rapid water currents of mountain streams are described and discussed...
August 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28802096/comparison-of-cleaning-methods-for-delicate-insect-specimens-for-scanning-electron-microscopy
#4
Katharina Schneeberg, René Bauernfeind, Hans Pohl
The objective of the present study was to compare cleaning methods for delicate insect specimens for investigations with scanning electron microscopy (SEM). As typical specimens we used aquatic larvae of mosquitoes, springtails, larvae of mayflies and caterpillars because they are very fragile and large parts of their body consist of soft tissue. Additionally their cuticle is very often covered with dirt, soil particles or other materials. Cleaning with ultrasonic sound, as the most common cleaning method used for SEM, will destroy fragile insects...
August 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28802083/phase-contrast-hard-x-ray-microscopy-using-synchrotron-radiation-for-the-properties-of-skeletal-muscle-in-mouse-hind-limbs
#5
Sanghun Jang, Jaehong Lim, Onseok Lee
Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies...
August 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28799679/scanning-electron-microscope-investigation-on-the-process-of-healing-of-skin-wounds-in-cirrhinus-mrigala
#6
Neeraj Verma, Usha Kumari, Swati Mittal, Ajay Kumar Mittal
Present scanning electron microscope study, reports healing of excised skin wounds in Cirrhinus mrigala. Healing process of wounds, inflicted on head skin, using biopsy punch was observed at intervals-0 hour (h), 1, 2, 4, 6, 12 h, 1 day (d) 2 and 4 d. Accumulation of mucus in wound region within 1h after infliction of wound has been considered an immediate measure to provide protection to injured skin from microbial invasion and other external environmental hazards. On infliction of wound, mobilization of epithelial cells at wound edge is associated with disturbance of coaptive relationship of epithelial cells till original coaptive stability is reached...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28799678/processing-and-characterization-of-canine-mixed-mammary-tumor-using-transmission-electron-microscopy
#7
Beltrán Audrey, Debut Alexis, Vaca Andrea, Ortiz Julio, Proaño-Pérez Freddy
Canine mammary gland tumors represent the second most frequent type of neoplasm in dogs, being an important problem within veterinary medical field. Canine mixed mammary tumors are the most common; the use of a transmission electron microscope (TEM) can contribute as a tool in its diagnosis by determining the characteristics of cellular components from numerous neoplasms. The aim of this study was to characterize cytologically canine mammary mixed tumor by the use of the TEM. A biopsy collected from an 11 years old bitch Shih-Tzu and analyzed by histopathology was used for ultrastructural analysis...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28799676/morphology-visualization-of-irregular-shape-bacteria-by-electron-holography-and-tomography
#8
Eduardo Ortega, Jesús Cantu-Valle, Germán Plascencia-Villa, Sandra Vergara, Fernando Mendoza-Santoyo, Alejandra Londono-Calderon, Ulises Santiago, Arturo Ponce
In the current work, irregular morphology of Staphylococcus aureus bacteria has been visualized by phase retrieval employing off-axis electron holography (EH) and 3D reconstruction electron tomography using high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM). Bacteria interacting with gold nanoparticles (AuNP) acquired a shrunken or irregular shape due to air dehydration processing. STEM imaging shows the attachment of AuNP on the surface of cells and suggests an irregular 3D morphology of the specimen...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28799674/ultrastructural-characteristics-of-ovine-bone-marrow-derived-mesenchymal-stromal-cells-cultured-with-a-silicon-stabilized-tricalcium-phosphate-bioceramic
#9
Salvatore Desantis, Gianluca Accogli, Janina Burk, Sara Zizza, Maria Mastrodonato, Edda G Francioso, Roberta Rossi, Antonio Crovace, Leonardo Resta
Bioceramics are being used in experimental bone engineering application in association with bone marrow derived mesenchymal stem cells (BM-MSCs) as a new therapeutic tool, but their effects on the ultrastructure of BM-MSCs are yet unknown. In this study we report the morphological features of ovine (o)BM-MSCs cultured with Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (SiTCP), able to promote the repair of induced bone defect in sheep model. oBM-MSCs were isolated from the iliac crest, cultured until they reached near-confluence and incubated with SiTCP...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28758716/tif4-gel-effects-on-tubular-occlusion-of-eroded-abraded-human-dentin
#10
Taís Fonseca Mantilla, Camila Vieira da Silva, Thayanne Monteiro Ramos-Oliveira, Cecilia Pedroso Turssi, Martha Simões Ribeiro, Patricia Moreira de Freitas
This in situ study evaluated the tubular occlusion caused by 4% TiF4 gel on the surface of eroded/abraded dentin. Sixty human dentin samples were eroded in vitro and assigned into six groups (n = 10) according to the in situ surface treatment and number of cycling days: 4% TiF4 gel applied once (TiF4 1), twice (TiF4 2), or three times (TiF4 3) followed by 2, 4, and 6 days of erosive/abrasive in situ cycling, respectively. Control groups (no treatment) were subjected to 2 (C1), 4 (C2), and 6 (C3) days of erosive/abrasive in situ cycling only...
July 31, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28752939/effect-of-organic-silicon-methylsulfonylmethane-and-glucosamine-sulfate-in-mandibular-bone-defects-in-rats
#11
Flávia Santiago de Sousa Rosa, Rúbia Teodoro Stuepp, Filipe Modolo, Michelle Tillmann Biz
Organic silicon (OS), glucosamine sulfate (GS), and methylsulfonylmethane (MSM) have been related to bone and connective tissue health and have been considered as basic therapy for osteoarthrosis disorders. Therefore, the aim was to analyze the effect of the association of these three components in mandibular bone defects in rats. Nine rats were used for histocompatibility test. In each animal was implanted the composition (70% OS, 15% GS, 15% MSM) and gutta percha (control) under the dorsal subcutaneous tissue...
July 28, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28742256/effect-of-chenopodium-ambrosioides-on-the-healing-process-of-the-in-vivo-bone-tissue
#12
Elizandra Silva da Penha, Rogério Lacerda-Santos, Maria Goretti Freire de Carvalho, Patrícia Teixeira de Oliveira
The focus of this double-blind randomized study was on evaluating the effect of an aqueous extract of Mastruz (Chenopodium ambrosioides L.) on the bone repair process in vivo. In total, 36 male Wistar rats were randomly selected for this study, and divided into 3 groups (n = 12): Group HS (Hemostatic Sponge), Group SM (Hemostatic Sponge with Mastruz) and Group BC (Blood Clot). In each animal, bone defects measuring 2 mm in diameter were performed in both tibias for placement of the substances. After 3 and 10 days, the animals were sacrificed, and the tissues were analyzed under an optical microscope relative to the following events: inflammatory infiltrate; necrosis; young fibroblasts; osteoclastic and osteoblastic activity; endosteal and periosteal bone formation; and bone repair...
July 25, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28742227/melatonin-prevents-mitochondrial-dysfunctions-and-death-in-differentiated-skeletal-muscle-cells
#13
Sara Salucci, Michela Battistelli, Valentina Baldassarri, Debora Burini, Elisabetta Falcieri, Sabrina Burattini
Oxidative stress increase induces cellular damage and apoptosis activation, a mechanism believed to represent a final common pathway correlated to sarcopenia and many skeletal muscle disorders. The goal of this study is to evaluate if melatonin, a ROS scavenger molecule, is able to counteract or modulate myotube death. Here, differentiated C2C12 skeletal muscle cells have been treated with melatonin before chemicals known to induce apoptotic death and oxidative stress, and its effect has been investigated by means of morpho-functional analyses...
July 25, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28730614/synchrotron-tomographic-images-from-human-lung-adenocarcinoma-three-dimensional-reconstruction-and-histologic-correlations
#14
Eunjue Yi, Sung-Mi Han, Ji-Eun Chang, Hong-Tae Kim, Jong-Ki Kim, Seung-Jun Seo, Jin-Haeng Chung, Sanghoon Jheon
High-resolution tomographic images using synchrotron X-rays are expected to provide detailed reflection of microstructures, thereby allowing for the examination of histologic structures without destruction of the specimen. This study aims to evaluate the synchrotron tomographic images of mixed ground-glass opacity excised on 5-mm sections in comparison to pathologic examination. The Institutional Review Board of our institute approved this retrospective study, and written informed consent was obtained from each patient whose lung tissue would be used...
July 21, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28699659/scanning-electron-and-light-microscopy-of-foliar-epidermal-characters-a-tool-for-plant-taxonomists-in-the-identification-of-grasses
#15
Raees Khan, Mushtaq Ahmad, Muhammad Zafar, Asad Ullah
SEM and light microscopy are of special interest for biologist to observe various features of the living bodies. In the current study we observed the foliar epidermal micro-morphological characters of 44 grass species using SEM and Light microscopy to assess their taxonomic utility for taxonomists in the identification process. The aim of this study is to use the foliar epidermal structural variations in both upper and lower surfaces for identification of grasses. Significant diversity was observed in both qualitative and quantitative characters using SEM and Light microscopy...
July 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28699651/combination-of-thresholding-and-fitting-methods-for-measuring-nanoparticle-sizes-and-size-distributions-in-s-tem
#16
Dmitry O Shvedchenko, Elena I Suvorova
The practical need for a simple and reliable tool for routine size analysis of nanoparticles with diameters down to a few nm embedded in a polymer matrix motivated the development of a new approach. The idea underlying the method proposed in this work is to combine intensity thresholding and contrast fitting procedures in the same software for particle recognition and measurements of sizes and size distributions of nanoparticles in transmission and scanning transmission electron microscopy images. Particle recognition in images is performed in an interactive process of manual setting the numerical threshold level after image preprocessing...
July 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28685923/interactions-and-effects-of-metal-oxide-nanoparticles-on-microorganisms-involved-in-biological-wastewater-treatment
#17
Pabel Cervantes-Avilés, Enrique Díaz Barriga-Castro, Lourdes Palma-Tirado, Germán Cuevas-Rodríguez
To clarify the toxicological effects of metal oxide nanoparticles (NPs) on microorganisms with environmental relevance, it is necessary to understand their interactions. In this work, they were studied the effects and the morphological interactions of two metal oxide NPs (ZnO and TiO2 ) with microorganisms, during aerobic treatment of wastewater. The effects were evaluated according to nutrient removal from wastewater, while morphological interactions were determined by three different techniques such as TEM, HAADF-STEM, as well as an elemental mapping...
July 7, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28675585/a-new-method-using-scanning-electron-microscopy-sem-for-preparation-of-anisopterous-odonates
#18
Alejandro Del Palacio, Patricia Laura Sarmiento, Muzón Javier
Anisopterous odonate male's secondary genitalia is a complex of several structures, among them the vesica spermalis is the most informative with important specific characters. The observation of those characters, mostly of membranous nature, is difficult in the Scanning Electron Microscope due to dehydration and metallization processes. In this contribution, we discuss a new and low cost procedure for the observation of these characters in the SEM, compatible with the most common agents used for preserving specimens...
July 4, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28661057/a-fast-autofocus-sharpness-function-of-microvision-system-based-on-the-robert-function-and-gauss-fitting
#19
Xiaopeng Sha, Pu Wang, Peng Shan, Huiguang Li, Zhiquan Li
For the microvision system, a new autofocus evaluation function based on the Robert function is proposed by increasing the threshold value. Compared with the traditional evaluation function, the new focus function reduces the local extreme value and increases the steepness of the focusing curve. According to the characteristics of the focusing evaluation function, the focus curve can be divided into two stages: the gentle area and the steep area. In the gentle area, there will be set a large step-length to realize the fast search...
June 29, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28653483/morphology-of-laryngeal-cartilage-of-the-nine-banded-armadillo-dasypus-novemcinctus-linnaeus-1758
#20
Clarisse Maria Barbosa Fonseca, Andrezza Braga Soares da Silva, Maria Michele Araújo de Sousa Cavalcante, Ingrid Macedo de Oliveira, Selma Maria Santos Moura, Bruno Melo Cunha, Carla Maria de Carvalho Leite, Maria Acelina Martins de Carvalho, Waldilleny Ribeiro de Araújo Moura, Márcia Dos Santos Rizzo, Airton Mendes Conde Júnior
Armadillos, Xenarthras representatives, known for adaptability to different ecosystems, own specific morphophysiological characteristics that are not known and deserve to be studied. The aim of this study was to describe the morphology of cartilage of the larynx of the nine-banded armadillo (Dasypus novemcinctus). Five dead armadillos were donated by the Chico Mendes Institute of Biodiversity (ICMBio-PI) to the Federal University of Piauí. The animals were fixed and dissected for removal of the larynx. The cartilages were identified and described, photodocumented, and schematized...
June 27, 2017: Microscopy Research and Technique
journal
journal
30945
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"