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Microscopy Research and Technique

Liliya Harri
The process of making of flexographic printing plate by the digital method proceeds in the following stages: back exposure, laser irradiation, main exposure, washing, post exposure, and finishing of the photopolymer plate. Laser irradiation is one of the basic technological stages, in which a negative of the reproduced image is created on the black layer of photopolymer plate. The article presents the results of studies of the effect laser irradiation parameters on the quality of flexographic printing plate produced by digital method...
October 24, 2016: Microscopy Research and Technique
Rackel Hatice Milhomens Gualberto Erduran, Fernando Akio Maeda, Sandra Regina Mota Ortiz, Tarcila Triviño, Acácio Fuziy, Paulo Eduardo Guedes Carvalho
The present study aimed to evaluate the precision of the torque applied by 0.022" self-ligating brackets of different brands, the precision of parallelism between the inner walls of their slots, and precision of their slot height. Eighty brackets for upper central incisors of eight trademarked models were selected: Abzil, GAC, American Orthodontics, Morelli, Orthometric, Ormco, Forestadent, and Ortho Organizers. Images of the brackets were obtained using a scanning electron microscope (SEM) and these were measured using the AutoCAD 2011 software...
October 22, 2016: Microscopy Research and Technique
Üzeyir Dogan, Hakan Çiftçi, Demet Cetin, Zekiye Suludere, Ugur Tamer
Transmission electron microscopy (TEM) is a very useful and commonly used microscopy technique, used especially for the characterization of nanoparticles. However, the identification of the magnetic nanoparticle could be thought problematic in TEM analysis, due to the fact that the magnetic nanoparticles are usually form aggregates on the TEM grid to form bigger particles generating higher stability. This prevents to see exact shape and size of each nanoparticle. In order to overcome this problem, a simple process for the formation of well-dispersed nanoparticles was conducted, by covering chitosan film on the unmodified copper grid, it was said to result in aggregation-free TEM images...
October 14, 2016: Microscopy Research and Technique
Khaled M Yassien, Mostafa Agour
A method for studying the effect of temperature on the optical properties of basalt fiber is presented. It is based on recording a set of phase-shifted digital holograms for the sample under the test. The holograms are obtained utilizing a system based on Mach-Zehnder interferometer, where the fiber sample inserted in an immersion liquid is placed within a temperature controlled chamber. From the recorded digital holograms the optical path differences which are used to calculate the refractive indices are determined...
October 11, 2016: Microscopy Research and Technique
Yan Wu, XiaoNing Guo, WeiLin Wang, XiangLong Chen, Zhijun Zhao, Xiumin Xia, YiMin Yang
Investigation of mortuary ritual is an important method to reconstruct many aspects of past societies. Due to the lack of relevant analytical work, little evidence related to organic materials in a burial can be found in China. Here we report materials collected from a burial during the excavation of the Shengedaliang site. The recovered materials were analyzed using Raman spectroscopy and plant analysis: flotation, pollen and phytolith analysis. The red pigments found scattered over the human remains were identified as cinnabar...
October 11, 2016: Microscopy Research and Technique
Yaroslav O Kamenev, Igor Y Dolmatov
The regeneration of the anterior portion of the body after fission was studied in the holothurian Cladolabes schmeltzii using electron microscopy methods. Following fission, the posterior portion of the digestive tube, cloaca, and respiratory trees remain in the posterior fragment of the body. The regeneration comprises five stages. In the first stage, connective-tissue thickening (an anlage of the aquapharyngeal bulb) occurs on the anterior end between the torn-off ends of the ambulacra. Most of the lost anterior organs developed in the second and third stages...
October 7, 2016: Microscopy Research and Technique
Amilton Cesar Dos Santos, Diego Carvalho Viana, Gleidson Benevides Oliveira, Renata Santos Silva, Moacir Franco Oliveira, Antônio Chaves Assis-Neto
The current study aimed to determine if characteristics observed in vaginal cytology during the estrous cycle of female SYT cavies corresponded with proliferation of the vaginal epithelium, characterized by proliferating cell nuclear antigen (PCNA) immunolocalization, and with follicular development at different phases of the estrous cycle. After determining estrous cycle phases by vaginal cytology, females were euthanized at metestrus, diestrus, proestrus, and estrus. Histological study of the vaginal epithelium and ovary were then performed...
October 7, 2016: Microscopy Research and Technique
Irmak Polat, Zekiye Suludere, Selami Candan
The morphology and ultrastructure of the rectum in Poecilimon cervus Karabağ, 1950 (Orthoptera, Tettigoniidae) were analyzed by light microscope, scanning (SEM) and transmission electron microscopes (TEM). The rectum is the final part of the digestive tract that plays an important role in water reabsorption in insects and so provides osmoregulation. In the transverse sections, six rectal pads and columnar epithelium can be distinguished. The cuticular intima lines the lumen at the apical side of the epithelium...
October 6, 2016: Microscopy Research and Technique
Matthew P Hoffman, Erik N Taylor, George E Aninwene, Sakthivel Sadayappan, Richard J Gilbert
Contraction of muscular tissue requires the synchronized shortening of myofibers arrayed in complex geometrical patterns. Imaging such myofiber patterns with diffusion-weighted MRI reveals architectural ensembles that underlie force generation at the organ scale. Restricted proton diffusion is a stochastic process resulting from random translational motion that may be used to probe the directionality of myofibers in whole tissue. During diffusion-weighted MRI, magnetic field gradients are applied to determine the directional dependence of proton diffusion through the analysis of a diffusional probability distribution function (PDF)...
October 2, 2016: Microscopy Research and Technique
Youjin Cheong, Young Jin Kim, Heeyoon Kang, Samjin Choi, Hee Joo Lee
We introduce a label-free spectroscopic method to classify subtypes of quinolone-nonsusceptible Escherichia coli (E. coli) isolates obtained from human blood cultures. Raman spectroscopy with a 30-nm gold-deposited, surface-enhanced Raman scattering (SERS) substrate was used to evaluate three multilocus sequencing typing (MLST)-predefined groups including E. coli ATCC25922, E. coli ST131:O75, and E. coli ST1193:O25b. Although there was a coffee-ring effect, the ring zone was selected at the ideal position to screen E...
October 2, 2016: Microscopy Research and Technique
Toshiko Yamazawa, Naotoshi Nakamura, Mari Sato, Chikara Sato
Exocrine glands, e.g., salivary and pancreatic glands, play an important role in digestive enzyme secretion, while endocrine glands, e.g., pancreatic islets, secrete hormones that regulate blood glucose levels. The dysfunction of these secretory organs immediately leads to various diseases, such as diabetes or Sjögren's syndrome, by poorly understood mechanisms. Gland-related diseases have been studied by optical microscopy (OM), and at higher resolution by transmission electron microscopy (TEM) of Epon embedded samples, which necessitates hydrophobic sample pretreatment...
October 2, 2016: Microscopy Research and Technique
Aleksandra Urbanek, Dorota Łuszczek, Małgorzata Kapusta
Acanthoscelides obtectus, a serious pest with a worldwide distribution, damages stored seeds of Phaseolus sp. To acquire a better understanding of the sensory orientation of bean weevils, their antennal and palpal sensilla were investigated. The antennae and palps possess several types of sensory hairs: blunt-tipped sensilla chaetica and trichoidea and also sharp-tipped sensilla trichoidea, these last being the most numerous. Sensilla basiconica are present only on the flagellomeres, but Böhm bristles occur on the basal sclerite and scape of the antennae...
October 2, 2016: Microscopy Research and Technique
Núria Gavara
Atomic Force microscopy (AFM) is becoming a prevalent tool in cell biology and biomedical studies, especially those focusing on the mechanical properties of cells and tissues. The newest generation of bio-AFMs combine ease of use and seamless integration with live-cell epifluorescence or more advanced optical microscopies. As a unique feature with respect to other bionanotools, AFM provides nanometer-resolution maps for cell topography, stiffness, viscoelasticity, and adhesion, often overlaid with matching optical images of the probed cells...
September 27, 2016: Microscopy Research and Technique
Taras Kotyk
: The objective of study is to study the peculiarities of morphological changes in different subdivisions of the intralobular duct of the submandibular gland (SMG) in rats in case of experimental diabetes mellitus (DM). The study included sexually mature male Wistar rats. Experimental DM was induced by streptozotocin. Electron microscopic study of subdivisions of the intralobular duct of the SMG was carried out on the 14th, 28th, 42nd, 56th, and 70th days of the experiment. In early stages of experimental DM the intercalated ducts are characterized by a relatively unchanged structure, and in late stages vacuolization of the cytoplasm of their epithelial cells is observed...
September 20, 2016: Microscopy Research and Technique
Makbule Bilge Akbulut, Pembegul Uyar Arpaci, Ayce Unverdi Eldeniz
The aim of this study was to evaluate the adhesion of periodontal ligament fibroblasts (PDLs) on newly proposed root repair materials [Biodentine, MM-MTA, polymethylmethacrylate (PMMA) bone cement, and SDR], in comparison with contemporary root repair materials [IRM, Dyract compomer, ProRoot MTA (PMTA), and Vitrebond]. Five discs from each material were fabricated in sterile Teflon molds, and the specimens were aged and prewetted in cell culture media for 96 hours. Three material discs were used for scanning electron microscopy (SEM) for the assessment of the attachment, density, and morphological changes in the PDLs, while two samples were used for energy dispersive x-ray spectroscopy (SEM-EDX) to determine the elemental composition of the materials...
September 20, 2016: Microscopy Research and Technique
Hui Deng, JiaHong Ning, XiaoNing Wang
An amino-functionalized sorbent (ACF) was prepared by grafting amine group onto cotton fiber (CF) using tetraethylenepentamine (TEPA). The resulting composite was characterized using various instrumental techniques such as scanning electron microscopy (SEM), Fourier transformed infrared (FTIR) and pHpzc analysis. FTIR spectrum and pHpzc analysis illustrated the involvement of amine groups in the X-3B sorption process. ACF sorbent was tested for its ability to remove X-3B from an aqueous solution in batch experiments...
September 17, 2016: Microscopy Research and Technique
Veronica Bonazza, Elisa Borsani, Barbara Buffoli, Stefania Castrezzati, Rita Rezzani, Luigi Fabrizio Rodella
Platelet concentrates, such as Concentrated Growth Factors (CGF), are autologous preparations obtained from the patient's own blood and rich in platelets, growth factors and cytokines involved in the key processes of tissue regeneration. These autologous concentrates differ in the way of preparation and also in the content of platelets, growth factors and leucocytes, as well as in the fibrin network architecture. So it is difficult to have a standardized product. The aim of the present study was to evaluate how the use of test tubes of different material, for blood collection, could influence the CGF production...
September 17, 2016: Microscopy Research and Technique
Ştefan Ţălu, Miroslaw Bramowicz, Slawomir Kulesza, Vali Dalouji, Shahram Solaymani, Shahoo Valedbagi
This work analyses the three-dimensional (3-D) surface texture of carbon-nickel (C-Ni) films grown by radio frequency (RF) magnetron co-sputtering on glass substrates. The C-Ni thin films were deposited under different deposition times, from 50 to 600 s, at room temperature. Atomic force microscopy was employed to characterize the 3-D surface texture data in connection with the statistical, and fractal analyses. It has been found that up to 180 s the sputtering occurs in more metal content mode and in greater than 180 s it occurs in more non-metal content mode...
September 17, 2016: Microscopy Research and Technique
Yan-Kun Cao, Min Huang
There are direct relationships between the behavioral mechanisms and sensilla. To obtain a better understanding of the behavioral mechanisms in Omosita colon (Linnaeus) (Coleoptera: Nitidulidae), we investigated the types, quantities, and distribution of sensilla on the antenna and mouthparts of O. colon by scanning electron microscopy. The clavate antenna comprised the scape, pedicel, and nine segment flagellomeres and had six types of sensilla, including two subtypes of sensilla chaetica (SC), three subtypes of sensilla basiconica (SB) and sensilla trichodea (ST), and one type of sensilla cavity, sensilla styloconica, and Böhm bristles (BB)...
September 15, 2016: Microscopy Research and Technique
Flaviana Soares Rocha, Pedro Henrique Justino Oliveira Limirio, Darceny Zanetta-Barbosa, Jonas Dantas Batista, Paula Dechichi
The deleterious effects of ionizing radiation on the growth plate continue to be cause for concern. This study evaluated the ionizing radiation effects on bone development and growth plate in the tibia of rats. All animals were submitted to ionizing radiation on the left leg. The animals were divided into two groups and euthanized 30 and 60 days after radiation. The tibiae were removed and separated into groups: control 30 days, irradiated 30 days, control 60 days and irradiated 60 days. Animals in each group (n = 7) were used for macroscopic and histological analysis...
September 2, 2016: Microscopy Research and Technique
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