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Microscopy Research and Technique

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https://www.readbyqxmd.com/read/27901295/nonparametric-de-noising-filter-optimization-using-structure-based-microscopic-image-classification
#1
Samsad Beagum, Nilanjan Dey, Amira S Ashour, Dimitra Sifaki-Pistolla, Valentina E Balas
The Local Polynomial Approximation (LPA) is a nonparametric filter that performs pixel-wise polynomial fit on a certain neighborhood. This filter can be supported by the Intersection of Confidence Interval rule (ICI) as an adaptation algorithm to identify the most suited neighborhood at which the polynomial assumptions provide superior fit for the observations. However, the LPA-ICI is considered to be a near-optimal de-noising filter. Moreover, the ICI rule has several parameters that affect its performance...
November 30, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27891737/role-of-resistance-physical-exercise-in-preventing-testicular-damage-caused-by-chronic-ethanol-consumption-in-uchb-rats
#2
Gessica Dutra Gonçalves, Nichelle Antunes Vieira, Henrique Rodrigues Vieira, Aline Dias Valério, Gláucia Elóisa Munhoz de Lion Siervo, Patricia Fernanda Felipe Pinheiro, Francisco Eduardo Martinez, Flávia Alessandra Guarnier, Giovana Rampazzo Teixeira, Glaura Scantamburlo Alves Fernandes
Ethanol consumption is associated with spermatogenesis damage and testosterone level alterations. Alcohol remains the most commonly used substance among athletes and sports enthusiasts. This study evaluated whether resistance physical exercise can reduce the testicular damage caused by ethanol exposure. A total of 36 ethanol drinking (UChB) rats were divided into four groups: C (control rats), ETOH (ethanol consumption), ETOH + T (ethanol consumption + physical training), and T (group physical training)...
November 28, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27878896/bonding-performance-of-universal-adhesives-to-er-cr-ysgg-laser-irradiated-enamel
#3
Muhammet Kerim Ayar, Fatih Erdemir
Universal adhesives have been recently introduced for use as self-etch or etch-and-rinse adhesives depending on the dental substrate and clinical condition. However, their bonding effectiveness to laser-irradiated enamel is still not well-known. Thus, the aim of this study was to compare the shear bond strength (SBS) of universal adhesives (Single Bond Universal; Nova Compo-B Plus) applied to Er,Cr:YSGG laser-irradiated enamel with SBS of the same adhesives applied in self-etch and acid-etching modes, respectively...
November 23, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859871/antheraea-pernyi-silk-sericin-mediating-biomimetic-nucleation-and-growth-of-hydroxylapatite-crystals-promoting-bone-matrix-formation
#4
Zhuang Jiayao, Zhou Guanshan, Zhang Jinchi, Chen Yuyin, Zhu Yongqiang
Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations...
November 18, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859981/influence-of-acceleration-setting-reaction-by-halogen-light-curing-on-gic-dentin-interface-qualitative-analysis-by-sem
#5
Laysa Yoshioka, Tamara Kerber Tedesco, Ana Flávia Bissoto Calvo, Sabrina Sun Ha Kim, Anna Paula Vieira Ferreira Prado Malagrana, Daniela Prócida Raggio
The aim of this study was to evaluate by scanning electron microscope (SEM) photomicrographs the influence of application of halogen light-curing for fastening the set reaction of high-viscosity glass ionomer cements (GIC) by assessing the material/dentin interface. Twelve human primary canines were assigned in four groups (n = 3) according to the GIC (Fuji IX, GC or Maxxion R, FGM) and application of halogen light-curing (60 sec or control-no external energy). Blocks with approximately 6 mm of height were buildup on previously pre-treated dentin surface in according to the experimental group...
November 17, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859883/atomic-force-microscopy-and-lamins-a-review-study-towards-future-combined-investigations
#6
REVIEW
Ilaria Pecorari, Luca Puzzi, Orfeo Sbaizero
In the last decades, atomic force microscopy (AFM) underwent a rapid and stunning development, especially for studying mechanical properties of biological samples. The numerous discoveries relying to this approach, have increased the credit of AFM as a versatile tool, and potentially eligible as a diagnostic equipment. Meanwhile, it has become strikingly evident that lamins are involved on the onset and development of certain diseases, including cancer, Hutchinson-Gilford progeria syndrome, cardiovascular pathologies, and muscular dystrophy...
November 17, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859881/towards-understanding-the-distribution-and-tumor-targeting-of-sericin-regulated-spherical-calcium-phosphate-nanoparticles
#7
Ruibo Zhao, Xiaoyuan Ren, Chungang Xie, Xiangdong Kong
The study of calcium phosphate (CaP) nanoparticles in vivo is still incomplete, which has limited their applications for biomedical delivery. Herein, we synthesized amorphous spherical calcium phosphate (S-CaP) nanoparticles with an average size of 80 nm via a co-precipitation method in the presence of silk sericin as the regulation template. S-CaP was labeled by the near-infrared dye reagent DiR, and then, the labeled nanoparticles (S-CaP@DiR) were used to investigate the distribution and degradation in healthy mice by IVIS and TEM...
November 17, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859863/the-influence-of-very-small-doses-of-alpha-radiation-on-the-stability-of-erythrocytes
#8
Magdalena Kaczmarska, Dominika Żydek, Justyna Wilkłacz-Potoczny, Maria Fornal, Tomasz Grodzicki, Elżbieta Kochowska, Krzysztof Kozak, Łukasz Gocal, Władysław Pohorecki, Krzysztof Matlak, Józef Korecki, Květoslava Burda
Our aim was to study the influence of low doses (0.2-4 μGy) of α radiation on the stability of human erythrocytes isolated from healthy and diabetic erythrocytes. Absorption spectroscopy was used to measure the level of red blood cell (RBC) hemolysis, along with Mössbauer spectroscopy, which is a highly specific method suited to monitoring various hemoglobin forms. States of hemoglobin are sensitive to a homeostatic imbalance in red blood cells. Changes in the membrane skeleton organization of irradiated erythrocytes isolated from healthy donors were studied using atomic force microscopy (AFM)...
November 16, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27862632/morphological-changes-induced-in-erythrocyte-membrane-by-the-antiepileptic-treatment-an-atomic-force-microscopy-study
#9
Bogdan Oprisan, Iuliana Stoica, Mihaela Iuliana Avadanei
Atomic force microscopy (AFM), a powerful characterization tool widely applied in problems in a large range of disciplines of the natural sciences, including cellular biology, was used to obtain information about the morphological changes induced in the erythrocyte membrane at the patients with epilepsy that undergo a long time treatment that operates upon one or several neuronal ionic channels, comparative with a healthy donor. This technique allowed non-invasive imaging of erythrocyte membrane, revealing details and specific characteristics down to the nanometer level with characterization of surface texture parameters, such as average height, average roughness and coefficient of kurtosis at micrometer/nanometer resolution...
November 12, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27862609/quantifying-the-growth-of-chlamydia-suis-in-cell-culture-using-high-content-microscopy
#10
Leentje De Puysseleyr, Kristien De Puysseleyr, Daisy Vanrompay, Winnok H De Vos
The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation...
November 12, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859898/structural-and-ultrastructural-features-of-the-malpighian-tubules-of-dolycoris-baccarum-linnaeus-1758-heteroptera-pentatomidae
#11
Nurcan Özyurt, Damla Amutkan, Irmak Polat, Tenay Kocamaz, Selami Candan, Zekiye Suludere
The morphology and ultrastructure of the Malpighian tubules of Dolycoris baccarum were analyzed by scanning (SEM) and transmission (TEM) electron microscopy in order to determine their functional organization. The Malpighian tubules are compared with similar structures of other insects based on cell structure and functional organization. The Malpighian tubules of D. baccarum extend from the midgut-hindgut region of the digestive tract. The Malpighian tubules are divided into two regions: the proximal segment is short and flattened and the distal segment is long, stringy in shape and free in hemolymph...
November 11, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27862607/anterograde-and-retrograde-tracing-with-high-molecular-weight-biotinylated-dextran-amine-through-thalamocortical-and-corticothalamic-pathways
#12
Wenjie Zhang, Dongsheng Xu, Jingjing Cui, Xianghong Jing, Nenggui Xu, Jianhua Liu, Wanzhu Bai
Biotinylated dextran amine (BDA) has been used for neural pathway tracing in the central nervous system for many decades, in which high molecular weight BDA appeared to be transported predominantly in the anterograde direction and less in the retrograde direction. In the current study, we reexamined the properties of neural labeling with high molecular weight BDA through a reciprocal neural pathway between thalamus and somatosensory cortex. After injection of BDA into the ventral posteromedial nucleus of thalamus (VPM) in the rat, the BDA labeling was sequentially examined on somatosensory cortex at 3, 5, 7, 10, and 14 survival days...
November 9, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859992/dependence-of-descriptors-of-co-localization-on-microscope-spatiotemporal-resolution-and-the-choice-of-regions-of-interest
#13
Maia Brunstein, Martin Oheim
The advent and increasing availability of super-resolution microscopies has prompted re-searchers to re-investigate questions of co-localization and co-clustering in the hope of providing more precise and relevant data. Here, we focus on the problem of studying inter-organelle interfaces, a topic of growing interest in cell biology. We sought to identify mitochondria-associated membrane (MAM) candidate sites from dual-colour large-field super-resolution images. MAMs are specialized lipid microdomains of the endoplasmic reticulum (ER) in close apposition with mitochondria...
November 8, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27813213/investigation-to-test-potential-stereolithography-materials-for-development-of-an-in-vitro-root-canal-model
#14
Saifalarab A Mohmmed, Morgana E Vianna, Stephen T Hilton, David R Boniface, Yuan-Ling Ng, Jonathan C Knowles
The aims were to compare the physico-chemical properties (zeta-potential, wettability, surface free energy) of stereolithography materials (STL) (Photopolymer, Accura) to dentine and to evaluate the potential of each material to develop Enterococcus faecalis biofilm on their respective surfaces. Eighteen samples of each test material (Photopolymer, Accura, dentine) were employed (total n = 54) and sectioned to 1 mm squares (5 mm x 5 mm) (n = 15) or ground into a powder to measure zeta-potential (n = 3)...
November 4, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27801529/a-histochemical-approach-to-glycan-diversity-in-the-urothelium-of-pig-urinary-bladder
#15
Maria Mastrodonato, Donatella Mentino, Angela Lopedota, Annalisa Cutrignelli, Giovanni Scillitani
Intracellular glycans in the urothelium of urinary bladder of 10 adult male Landrace pigs were characterized in situ by immunohistochemical detection of Muc1 mucin by anti MUC1 from rabbit, conventional histochemical techniques (Periodic-Acid Schiff, Alcian Blue pH 2.5, High-Iron Diamine), and binding with 13 lectins (PNA, DBA, RCA-I, WGA, SBA, BSI-B4, ConA, AAA, UEA-I, LTA, LFA, MAA-II, SNA) combined with chemical and enzymatic pre-treatments (β-elimination, desulfation and neuraminidase) to gather reference data for this model animal...
November 1, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27775840/microscopic-studies-of-the-effect-of-laser-irradiation-parameters-of-photopolymer-plate-on-the-quality-of-flexographic-printing-plate-produced-by-the-digital-method
#16
Liliya Harri
The process of making of flexographic printing plate by the digital method proceeds in the following stages: back exposure, laser irradiation, main exposure, washing, post exposure, and finishing of the photopolymer plate. Laser irradiation is one of the basic technological stages, in which a negative of the reproduced image is created on the black layer of photopolymer plate. The article presents the results of studies of the effect laser irradiation parameters on the quality of flexographic printing plate produced by digital method...
October 24, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27739130/nanoparticle-embedded-chitosan-film-for-agglomeration-free-tem-images
#17
Üzeyir Dogan, Hakan Çiftçi, Demet Cetin, Zekiye Suludere, Ugur Tamer
Transmission electron microscopy (TEM) is a very useful and commonly used microscopy technique, used especially for the characterization of nanoparticles. However, the identification of the magnetic nanoparticle could be thought problematic in TEM analysis, due to the fact that the magnetic nanoparticles are usually form aggregates on the TEM grid to form bigger particles generating higher stability. This prevents to see exact shape and size of each nanoparticle. In order to overcome this problem, a simple process for the formation of well-dispersed nanoparticles was conducted, by covering chitosan film on the unmodified copper grid, it was said to result in aggregation-free TEM images...
October 14, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27726234/measuring-opto-thermal-parameters-of-basalt-fibers-using-digital-holographic-microscopy
#18
Khaled M Yassien, Mostafa Agour
A method for studying the effect of temperature on the optical properties of basalt fiber is presented. It is based on recording a set of phase-shifted digital holograms for the sample under the test. The holograms are obtained utilizing a system based on Mach-Zehnder interferometer, where the fiber sample inserted in an immersion liquid is placed within a temperature controlled chamber. From the recorded digital holograms the optical path differences which are used to calculate the refractive indices are determined...
October 11, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27726230/red-pigments-and-boraginaceae-leaves-in-mortuary-ritual-of-late-neolithic-china-a-case-study-of-shengedaliang-site
#19
Yan Wu, XiaoNing Guo, WeiLin Wang, XiangLong Chen, Zhijun Zhao, Xiumin Xia, YiMin Yang
Investigation of mortuary ritual is an important method to reconstruct many aspects of past societies. Due to the lack of relevant analytical work, little evidence related to organic materials in a burial can be found in China. Here we report materials collected from a burial during the excavation of the Shengedaliang site. The recovered materials were analyzed using Raman spectroscopy and plant analysis: flotation, pollen and phytolith analysis. The red pigments found scattered over the human remains were identified as cinnabar...
October 11, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27717203/anterior-regeneration-after-fission-in-the-holothurian-cladolabes-schmeltzii-dendrochirotida-holothuroidea
#20
Yaroslav O Kamenev, Igor Y Dolmatov
The regeneration of the anterior portion of the body after fission was studied in the holothurian Cladolabes schmeltzii using electron microscopy methods. Following fission, the posterior portion of the digestive tube, cloaca, and respiratory trees remain in the posterior fragment of the body. The regeneration comprises five stages. In the first stage, connective-tissue thickening (an anlage of the aquapharyngeal bulb) occurs on the anterior end between the torn-off ends of the ambulacra. Most of the lost anterior organs developed in the second and third stages...
October 7, 2016: Microscopy Research and Technique
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