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Microscopy Research and Technique

Wen-Hao Wang, Jun Yang, Hua-Sheng Peng, Jiang-Ping Qian
Modern clinical studies have found that Pulsatilla radix contains a variety of active ingredients; however, its medicinal parts and microstructure have been controversial. Based on morphological observation and microscopic identification of different structures, we chose Anhui, Henan, Jilin, and Shanxi Province to study Pulsatilla chinensis (Bunge) Regel populations. We specifically addressed different diameters and anatomical structures of different parts of underground organs. We also found that P. chinensis (Bunge) Regel had a "joint point" structure in its underground organ...
June 16, 2017: Microscopy Research and Technique
Dai Hyun Kim, Ga Na Oh, In Hyuk Kwon, Soo Hong Seo, Young Chul Kye, Hyo Hyun Ahn
Aged skin is reported to be associated with unattractive skin color changes and solar elastosis. However, comparative studies have not documented the possible correlation between the two factors. This study investigated the plausible relationship between the facial skin color of elderly Asians and solar elastosis. A total of 22 skin specimens were collected from 22 Korean patients who underwent cheek skin biopsies. Skin color was quantitatively measured using colorimetric photography techniques to produce CIE L*a*b* values; the degree of solar elastosis was quantifiably assessed using a histologic grading scale...
June 11, 2017: Microscopy Research and Technique
Shouvik Chakraborty, Sankhadeep Chatterjee, Nilanjan Dey, Amira S Ashour, Ahmed S Ashour, Fuqian Shi, Kalyani Mali
Microscopic image analysis is one of the challenging tasks due to the presence of weak correlation and different segments of interest that may lead to ambiguity. It is also valuable in foremost meadows of technology and medicine. Identification and counting of cells play a vital role in features extraction to diagnose particular diseases precisely. Different segments should be identified accurately in order to identify and to count cells in a microscope image. Consequently, in the current work, a novel method for cell segmentation and identification has been proposed that incorporated marking cells...
May 30, 2017: Microscopy Research and Technique
N Yu Parkhimovich, A R Salikhov, M F Imayev
A graphical method for phase analysis of advanced materials by EDS-SEM was developed and demonstrated on deformed superconducting Bi(Pb)2223 ceramics. Through visual representation, this method allows for the rapid and efficient analysis of large X-ray microanalysis datasets and to identify phase composition of fine particles of secondary phases against a background of other phases. The graphical method can be applied using existing software and therefore does not require the development of new programs or complex computations...
May 27, 2017: Microscopy Research and Technique
Yulin Lin, Limin Ma, Ye Zhu, Zefeng Lin, Zilong Yao, Yu Zhang, Chuanbin Mao
There has not been a satisfying method to predict the fracture risk in tumorous bone lesions. To tackle this challenge, we used a finite element method to assess the fracture risk in the proximal tibia (pT) when the size and location of the tumorous defects are varied in bone. Towards this end, the circular cortical defects, mimicking the tumorous lesions by forming cortical window defects, with a diameter (Ф) of 20, 30, 40, or 50 mm, are structured on the anteromedial, lateral, posterior wall of pT, which is located 5, 15, and 25 mm below articular margin, respectively...
May 27, 2017: Microscopy Research and Technique
Carolina Andolfatto, Idomeo Bonetti-Filho, Iracilda Zeppone Carlos, Juliane Maria Guerreiro-Tanomaru, Milton Carlos Kuga, Fernanda Borges Cunha Tormin, Gisele Faria
The aim was to evaluate the cytocompatibility of the AH Plus and Sealapex associated with amoxicillin (AA) in a quantity of 10% by total weight of the sealers, the flow and setting time of these sealers with 10%-0.25% AA and antibiofilm activity of these sealers associated with AA in a concentration that does not alter their physical properties (1%). Cytocompatibility was assessed by MTT, neutral red and cytoskeletal fluorescence assays. Setting time and flow was evaluated using the specifications of ISO 6876/2012...
May 26, 2017: Microscopy Research and Technique
Genxia He, Junchao Qian
It was known that Spinal cord injury (SCI) leads to neuronal cell death, axonal damage and demyelination, and often results in severe functional loss. Noninvasive imaging of axonal integrity may hold great importance in clinic. This study evaluated the role of phase imaging in detection of axonal integrity of cranial corticospinal tract after spinal cord injury. Quantitative MR phase imaging and diffusion tensor imaging were performed on a group of rats prior to and after spinal cord injury at 9.4 T in this study...
May 23, 2017: Microscopy Research and Technique
Rafael Scaf de Molon, Celso Eduardo Sakakura, Rafael Silveira Faeda, Rafael Sartori, Daniel Palhares, Rogerio Margonar, Elcio Marcantonio
The aim of this study was to investigate the effect of cyclosporine A (CsA) therapy on bone healing around osseointegrated implants in the rabbit by means of descriptive histologic and histomorphometric analyses. Eighteen rabbits received one implant each in the right tibia proximal metaphysis. After the bone-healing period of 3 months, 6 randomly selected animals were sacrificed (Group CTL-12) before the immunosuppression therapy initiation to obtain a standard of implant integration. Another six randomly selected animals were submitted to a daily subcutaneous (sc) injection of 10 mg/kg CsA (Group CsA) whereas the six remaining animals received daily sc injections of saline solution (Group CTL-24)...
May 22, 2017: Microscopy Research and Technique
E R Lauriano, S Pergolizzi, J Gangemi, M Kuciel, G Capillo, M Aragona, C Faggio
Serotonin [5-hydroxytryptamine (5-HT)] is an important neuromodulator involved in a wide range of physiological functions. The effects of serotonin are mediated by an extended family of receptors coupled to multiple heterotrimeric G-proteins, associated with cellular membrane. G proteins connect receptors to effectors and thus trigger intracellular signaling pathways. These cellular processes several regulate systemic functions such as embryonic development, gonadal development, learning and memory, and organismal homeostasis...
May 22, 2017: Microscopy Research and Technique
Serhat Köseoğlu, Tuğba Pekbağr Yan K, Ebru Kucukyilmaz, Mehmet Sağlam, Sukru Enhos, Ayşe Akgün
This study evaluated four root repair materials for cytotoxicity and cell attachment in vitro. Cell viability was determined at 24 hr, 3 days and 7 days by using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay with material extracts. Cell adhesion was examined with a scanning electron microscope on the surface of materials at 24 and 48 hr. Angelus MTA displayed the lowest cell viabilities at all-day incubations. Endocem had high biocompatibility on the first day. After culturing for 3 days and 7 days, the cell viabilities of Biodentine, Endocem and MM-MTA had similar results, and their cell viability was significantly higher than that of Angelus...
May 15, 2017: Microscopy Research and Technique
Aashish Choudhary, Nitin Gupta, Hafiz Ahmad, Bijay Ranjan Mirdha
The modified acid fast staining technique is a commonly used procedure for the detection of coccidian parasites in developing countries. The morphological variations observed in these parasites play a significant role to some extent in both identification and diagnosis of these parasitic infections. A prospective cross sectional study was performed over three years. The fecal smears were stained by modified Kinyoun acid-fast staining technique and were extensively studied for morphological variations in the coccidian parasites...
May 12, 2017: Microscopy Research and Technique
Young-Ran Ha, Jeongeun Ryu, Eunseop Yeom, Sang-Joon Lee
Mosquito-borne diseases, such as malaria, dengue fever, and Zika virus, are serious global health issues. Vector control may be an important strategy in reducing the mortality caused by these diseases. The respiratory system of mosquito larvae in the water has to inhale atmospheric oxygen as aquatic organisms. In this study, the three-dimensional (3D) structures of the dorsal longitudinal trunks (DLTs) of the tracheal systems of Anopheles sinensis and Aedes togoi were compared using synchrotron X-ray microscopic computed tomography...
May 12, 2017: Microscopy Research and Technique
Cíntia Tereza Pimenta Araújo, Lúcia Trazzi Prieto, Daiane Cristianismo Costa, Mariana Avalone Bosso, Erick Kamiya Coppini, Carlos Tadeu Santos Dias, Luis Alexandre Maffei Sartini Paulillo
AIM: Evaluate the composite-to-enamel bond after passive or active application of self-etching primer systems on polished or pre-etched enamel with phosphoric acid. MATERIALS AND METHODS: Two self-etch adhesives systems (SEAS) were used: Clearfil SE Bond and Easy Bond. Third human molars were divided into 8 groups (N = 10). The crown of each tooth was sectioned into halves and the mesial/distal surfaces were used. The adhesives were actively or passively applied on enamel with or without prior phosphoric-acid etching...
May 10, 2017: Microscopy Research and Technique
Masahiro Kawasaki, Tadashi Nobuchi, Yuta Nakafushi, Masateru Nose, Masateru Shibata, Peng Li, Makoto Shiojiri
Plant anatomy was examined for two clarinet reeds made out of Arundo donax by different means of microscopy: light microscopy, low-energy secondary electron scanning electron microscopy (SEM), backscattered electron SEM, and helium ion microscopy (HiM). The local indentation hardness HIT and Young's modulus EIT of different tissues on their cross sections were measured. A vascular bundle (Vb) (HIT  = 60-100 MPa, EIT  = 1,500-2,000 MPa) that includes soft tissues of phloem and xylem and a vascular bundle sheath (Bs) (HIT  = 300-500 MPa, EIT  = ∼7,000 MPa) form a pipe of the strong string along the longitudinal direction of the cane...
May 9, 2017: Microscopy Research and Technique
Rodríguez Héctor, Espinoza-Navarro Omar, Mercedes González, Castro Mario
The El Plomo mummy was a pre-Columbian Incan child who was found mummified in the Andes Mountains above an altitude of 17,700 feet. In the environment, natural mummification occurred due to low temperatures and strong winds. Dating measurements (relative dating) by experts from the National Museum of Natural History of Chile established that the mummified body corresponds the Inca period (1,450 to 1,500 AD). In 2003, the body was transferred to the University of Chile Medical School for exhaustive medical examination...
May 5, 2017: Microscopy Research and Technique
Reza Fekrazad, Ghogha Lotfi, Mohammad Harandi, Sara Ayremlou, Katayoun A M Kalhori, Gholam Ali Gholami, Hanieh Nokhbatolfoghahaei
BACKGROUND AND OBJECTIVES: The regeneration of periodontal support is the main concern in periodontal therapy. The aim of this in vitro study was to investigate the fibroblasts attachment on root surfaces after scaling with Er, Cr:YSGG laser and ultrasonic instruments using scanning electron microscopy (SEM). METHODS: 72 root plates of ∼6 × 4 × 1 mm(3) in dimension were prepared from 27 single-rooted human mature teeth and were then divided into four groups...
May 3, 2017: Microscopy Research and Technique
Sayed Mahmood Rabiee, Seyedeh Zeinab Eftekhari, Valiollah Arash, Nadereh Amozegar, Alireza Fathi, Saeid Tavanafar, Ali Bijani
OBJECTIVE: This study aims at reducing frictional resistance of the ceramic brackets by using CO2 laser irradiation. METHODS: Forty-two polycrystalline Al2 O3 ceramic brackets were randomly divided into six groups of seven samples: a control group B0 (not subjected to laser irradiation) and five groups subjected to irradiation with intensities of 10 (B10), 30 (B30), 70 (B70), 90 (B90), and 110 (B110) J/cm(2) . After irradiation, two samples from each group were evaluated by scanning electron microscopy (SEM) and atomic force microscopy (AFM), while the remaining five samples were tested for frictional resistance...
May 3, 2017: Microscopy Research and Technique
Maja Jordanova, Katerina Rebok, Zrinka Dragun, Sheriban Ramani, Lozenka Ivanova, Vasil Kostov, Damir Valić, Nesrete Krasnići, Vlatka Filipović Marijić, Damir Kapetanović
Pollution with heavy metals may influence the immune system of fish, leading to impairment of their health or even increase their mortality. The fish kidney is one of the first fish organs to be affected by water contamination. Amounts of kidney macrophages (MACs), which are involved in fish immune response, as well as the qualitative and quantitative changes in the pigmented MACs in fish kidney, are used as biomarkers of pollution. Therefore, in this study, we have evaluated relative and total volumes of trunk kidney pigmented MACs, and analyzed the pigments accumulated within them...
April 28, 2017: Microscopy Research and Technique
Jana Liskova, Daniel Hadraba, Elena Filova, Miroslav Konarik, Jan Pirk, Karel Jelen, Lucie Bacakova
Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions; with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures...
April 28, 2017: Microscopy Research and Technique
Keiichi Moriguchi, Yoshiaki Hasegawa, Naoya Higuchi, Yukitaka Murakami, Fuminobu Yoshimura, Kazuhiko Nakata, Masaki Honda
We investigated the association between human polymorphonuclear leukocytes (PMNs) and non-opsonized Tannerella forsythia ATCC 43037 displaying a serum-resistant surface layer (S-layer). When PMNs were mixed with T. forsythia in suspension, the cells phagocytosed T. forsythia cells. Nitro blue tetrazolium (NBT) reduction, indicative of O2- production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H2 O2 production, was observed by electron microscopy. We examined the relationship between high-molecular-weight proteins of the S-layer and Ce reaction (for T...
April 24, 2017: Microscopy Research and Technique
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