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Microscopy Research and Technique

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https://www.readbyqxmd.com/read/28083993/algae-through-the-looking-glass
#1
REVIEW
Primo Coltelli, Laura Barsanti, Valter Evangelista, Paolo Gualtieri
Microalgae are one of the most suitable subjects for testing the potentiality of light microscopy and image analysis, because of the size of single cells, their endogenous chromaticity, and their metabolic and physiological characteristics. Microscope observations and image analysis can use microalgal cells from lab cultures or collected from water bodies as model to investigate metabolic processes, behavior/reaction of cells under chemical or photic stimuli, and dynamics of population in the natural environment in response to changing conditions...
January 13, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28059485/new-elements-in-the-interreceptor-matrix-a-comparative-study-of-megavilli-and-landolt-s-club
#2
Jingjin Yang, Sharon L Wu, Maggie S M Chow, Maria S M Wai, David T Yew
Megavilli and Landolt's clubs were reported in the interreceptor matrix of the retina of the goldfish and neonatal rat for the first time. Megavilli encroach onto the inner or outer segments of photoreceptors and were different from microvilli. Landolt's clubs were not present in the adult rat but only in the adult goldfish.
January 6, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28059480/polarization-microscopy-as-a-tool-for-quantitative-evaluation-of-collagen-using-picrosirius-red-in-different-stages-of-ckd-in-cats
#3
G B Morais, D A Viana, F M O Silva, F A F Xavier Júnior, K M Farias, C D'Ó Pessoa, J A M Silveira, A P N N Alves, M R L Mota, F D O Silva, C M S Sampaio, J M G Verdugo, J S A M Evangelista
Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization...
January 6, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28029189/morphological-and-immunohistochemical-analysis-of-the-biocompatibility-of-resin-modified-cements
#4
Janaina Almeida Mesquita, Rogério Lacerda-Santos, Gustavo Pina Godoy, Cassiano Franscisco Weege Nonaka, Pollianna Muniz Alves
The aim of this double-blind randomized study was to evaluate the biocompatibility of resin-modified glass ionomer cements (RMGIC) by means of morphological and immunohistochemical analyses. RMGICs were selected and divided into four groups: Group CK (Crosslink Orthodontic Band Cement); Group RS (Resilience Light Cure Band Cement) Group RMO (RMO Band Cement), Group TP (Transbond Plus Light Cure Band), and Group C (Control-polyethylene). The materials were implanted in rat subcutaneous tissues, randomly selected for this study...
December 28, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28001323/prenatal-transplantation-of-epidermal-neural-crest-stem-cells-in-malformation-of-cortical-development-mouse-model
#5
Ameneh Omidi, Mohammad Akbari, Keywan Mortezaee, Ehsan Eqlimi, Cordian Beyer, Adib Zendedel, Iraj Ragerdi Kashani
Prenatal interventions may offer an immense opportunity in therapeutic protocols of malformations of cortical development (MCD). Epidermal neural crest stem cells (EPI-NCSCs) of the hair follicle bulge exhibit features of both embryonic and adult stem cells; these cells maintain their neurologic differentiation capability because of their neural crest origin. However, it is unknown if prenatal use of EPI-NCSCs could be beneficial in targeting methylazoxymethanol (MAM)-induced MCD, which further addressed in the present work...
December 21, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28000307/outlook-on-optimizing-ultramicroscopy-imaging-technique-through-optical-characterization
#6
Saiedeh Saghafi, Klaus Becker, Christian Hahn, Marko Pende, Nina Jährling, Martina Wanis, Inna Sabdyusheva-Litschauer, Massih Foroughipour, Axel Niendorf, Hans-Ulrich Dodt
Here, we present an optically optimized system for static ultramicroscopy imaging technique. The unit for generating an ultra-thin light sheet employs aspheric and meso-optical elements (meso-aspheric system). An analytical as well as an experimental comparison between the light sheet produced by the standard system (using a rectangular slit aperture and one cylindrical lens) and the one produced by our latest optimized system, which converts a symmetrical Gaussian beam into an ultra-thin light sheet is presented...
December 20, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27990704/differentiation-of-bone-marrow-derived-stage-specific-embryonic-antigen-1-positive-pluripotent-stem-cells-into-male-germ-cells
#7
Reza Shirazi, Amir Hassan Zarnani, Masoud Soleimani, Karim Nayernia, Iraj Ragerdi Kashani
Studies published in recent years have changed the outlook on sterility and germ cell development by producing gametes from stem cells. In present study, a novel approach on differentiation of bone marrow-derived stage-specific embryonic antigen 1 positive (SSEA-1(+) ) pluripotent stem cells into male germ cells has been addressed. SSEA-1(+) stem cells were separated from murine bone marrow using magnetic-activated cell sorting (MACS) system and propagated on a feeder layer cells. To evaluate the pluripotency characteristic of the purified cells, they were differentiated toward cells of three germ layers...
December 19, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27935662/antibacterial-activity-of-glass-ionomer-cement-modified-by-zinc-oxide-nanoparticles
#8
Patrícia Petromilli Nordi Sasso Garcia, Mariana Florian Bell Cardia, Renata Serignoli Francisconi, Lívia Nordi Dovigo, Denise Madalena Palomari Spolidório, Alessandra Nara de Souza Rastelli, Ana Carolina Botta
This study evaluated the antibacterial activity of zinc oxide nanoparticles incorporated into self-cured glass ionomer cement (GIC) and light-cured resin-reinforced GIC on Streptococcus mutans biofilm. The GICs, Fuji II (GC America) and Fuji II LC (GC America), were incorporated with nanoparticles at concentrations of 0%, 1%, and 2% by weight, and the biofilm maturation time was one and seven days. Circular specimens of each GIC type were prepared. The antibacterial activity was evaluated by determining the number of colony forming units of S...
December 9, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27935660/biofilm-formation-in-haas-palatal-expanders-with-and-without-use-of-an-antimicrobial-agent-an-in-situ-study
#9
Cristhiane Ristum Bagatin, Marcela Cristina Damião Andrucioli, José Tarcísio Lima Ferreira, Mírian Aiko Nakane Matsumoto, Raquel Assed Bezerra da Silva, Lea Assed Bezerra da Silva, Fábio Lourenço Romano, Paulo Nelson-Filho
Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contamination by mutans streptococci (MS) of different surfaces of Haas palatal expanders with and without use of chlorhexidine gluconate mouthrinses (CHX). Thirty-four patients were randomly assigned to two groups (n = 17/group), using placebo (Group I) and 0...
December 9, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27935654/research-of-bionic-design-on-tools-with-rostrum-of-cyrtotrachelus-bugueti-guer-coleoptera-curculionidae
#10
Shun Xu, Longhai Li, Jin Tong, Mo Li
To improve the torsional resistance capability of multilayered composite pipes, a bionic design was presented based on the rostrum of Cyrtotrachelus Bugueti Guer. Morphology and mechanical properties of the weevil's rostrum were studied. Scanning electron microscope (SEM) was employed to observe the macro-/microstructure and inner structure. Nanoindentation was carried out to measure the elastic modulus and the hardness of the biological materials. Based on the structure of rostrum, a mathematical model of multilayered biomimetic pipe was established with the statistical methods...
December 9, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27921341/unexpected-effect-of-the-vehicle-grain-ethanol-of-homeopathic-fsh-on-the-in-vitro-survival-and-development-of-isolated-ovine-preantral-follicles
#11
Lartiza F Lima, Rebeca M P Rocha, Ana Beatriz G Duarte, Ivina R Brito, Gerlane M Silva, Giovanna Q Rodrigues, Diana C S Nunes-Pinheiro, Antônia D Sales, Arlindo A Moura, Matthew B Wheeler, Ana Paula R Rodrigues, Cláudio C Campello, José Ricardo Figueiredo
The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic - FSH 6cH and allopathic FSH - rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM(+) supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p < ...
December 5, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27901295/nonparametric-de-noising-filter-optimization-using-structure-based-microscopic-image-classification
#12
Samsad Beagum, Nilanjan Dey, Amira S Ashour, Dimitra Sifaki-Pistolla, Valentina E Balas
The Local Polynomial Approximation (LPA) is a nonparametric filter that performs pixel-wise polynomial fit on a certain neighborhood. This filter can be supported by the Intersection of Confidence Interval rule (ICI) as an adaptation algorithm to identify the most suited neighborhood at which the polynomial assumptions provide superior fit for the observations. However, the LPA-ICI is considered to be a near-optimal de-noising filter. Moreover, the ICI rule has several parameters that affect its performance...
November 30, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27891737/role-of-resistance-physical-exercise-in-preventing-testicular-damage-caused-by-chronic-ethanol-consumption-in-uchb-rats
#13
Gessica Dutra Gonçalves, Nichelle Antunes Vieira, Henrique Rodrigues Vieira, Aline Dias Valério, Gláucia Elóisa Munhoz de Lion Siervo, Patricia Fernanda Felipe Pinheiro, Francisco Eduardo Martinez, Flávia Alessandra Guarnier, Giovana Rampazzo Teixeira, Glaura Scantamburlo Alves Fernandes
Ethanol consumption is associated with spermatogenesis damage and testosterone level alterations. Alcohol remains the most commonly used substance among athletes and sports enthusiasts. This study evaluated whether resistance physical exercise can reduce the testicular damage caused by ethanol exposure. A total of 36 ethanol drinking (UChB) rats were divided into four groups: C (control rats), ETOH (ethanol consumption), ETOH + T (ethanol consumption + physical training), and T (group physical training)...
November 28, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27878896/bonding-performance-of-universal-adhesives-to-er-cr-ysgg-laser-irradiated-enamel
#14
Muhammet Kerim Ayar, Fatih Erdemir
Universal adhesives have been recently introduced for use as self-etch or etch-and-rinse adhesives depending on the dental substrate and clinical condition. However, their bonding effectiveness to laser-irradiated enamel is still not well-known. Thus, the aim of this study was to compare the shear bond strength (SBS) of universal adhesives (Single Bond Universal; Nova Compo-B Plus) applied to Er,Cr:YSGG laser-irradiated enamel with SBS of the same adhesives applied in self-etch and acid-etching modes, respectively...
November 23, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859871/antheraea-pernyi-silk-sericin-mediating-biomimetic-nucleation-and-growth-of-hydroxylapatite-crystals-promoting-bone-matrix-formation
#15
Zhuang Jiayao, Zhou Guanshan, Zhang Jinchi, Chen Yuyin, Zhu Yongqiang
Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations...
November 18, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859981/influence-of-acceleration-setting-reaction-by-halogen-light-curing-on-gic-dentin-interface-qualitative-analysis-by-sem
#16
Laysa Yoshioka, Tamara Kerber Tedesco, Ana Flávia Bissoto Calvo, Sabrina Sun Ha Kim, Anna Paula Vieira Ferreira Prado Malagrana, Daniela Prócida Raggio
The aim of this study was to evaluate by scanning electron microscope (SEM) photomicrographs the influence of application of halogen light-curing for fastening the set reaction of high-viscosity glass ionomer cements (GIC) by assessing the material/dentin interface. Twelve human primary canines were assigned in four groups (n = 3) according to the GIC (Fuji IX, GC or Maxxion R, FGM) and application of halogen light-curing (60 sec or control-no external energy). Blocks with approximately 6 mm of height were buildup on previously pre-treated dentin surface in according to the experimental group...
November 17, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859881/towards-understanding-the-distribution-and-tumor-targeting-of-sericin-regulated-spherical-calcium-phosphate-nanoparticles
#17
Ruibo Zhao, Xiaoyuan Ren, Chungang Xie, Xiangdong Kong
The study of calcium phosphate (CaP) nanoparticles in vivo is still incomplete, which has limited their applications for biomedical delivery. Herein, we synthesized amorphous spherical calcium phosphate (S-CaP) nanoparticles with an average size of 80 nm via a co-precipitation method in the presence of silk sericin as the regulation template. S-CaP was labeled by the near-infrared dye reagent DiR, and then, the labeled nanoparticles (S-CaP@DiR) were used to investigate the distribution and degradation in healthy mice by IVIS and TEM...
November 17, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27862632/morphological-changes-induced-in-erythrocyte-membrane-by-the-antiepileptic-treatment-an-atomic-force-microscopy-study
#18
Bogdan Oprisan, Iuliana Stoica, Mihaela Iuliana Avadanei
Atomic force microscopy (AFM), a powerful characterization tool widely applied in problems in a large range of disciplines of the natural sciences, including cellular biology, was used to obtain information about the morphological changes induced in the erythrocyte membrane at the patients with epilepsy that undergo a long time treatment that operates upon one or several neuronal ionic channels, comparative with a healthy donor. This technique allowed non-invasive imaging of erythrocyte membrane, revealing details and specific characteristics down to the nanometer level with characterization of surface texture parameters, such as average height, average roughness and coefficient of kurtosis at micrometer/nanometer resolution...
November 12, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27862609/quantifying-the-growth-of-chlamydia-suis-in-cell-culture-using-high-content-microscopy
#19
Leentje De Puysseleyr, Kristien De Puysseleyr, Daisy Vanrompay, Winnok H De Vos
The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation...
November 12, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859898/structural-and-ultrastructural-features-of-the-malpighian-tubules-of-dolycoris-baccarum-linnaeus-1758-heteroptera-pentatomidae
#20
Nurcan Özyurt, Damla Amutkan, Irmak Polat, Tenay Kocamaz, Selami Candan, Zekiye Suludere
The morphology and ultrastructure of the Malpighian tubules of Dolycoris baccarum were analyzed by scanning (SEM) and transmission (TEM) electron microscopy in order to determine their functional organization. The Malpighian tubules are compared with similar structures of other insects based on cell structure and functional organization. The Malpighian tubules of D. baccarum extend from the midgut-hindgut region of the digestive tract. The Malpighian tubules are divided into two regions: the proximal segment is short and flattened and the distal segment is long, stringy in shape and free in hemolymph...
November 11, 2016: Microscopy Research and Technique
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