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Microscopy Research and Technique

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https://www.readbyqxmd.com/read/28439996/energy-dispersive-spectroscopy-scanning-transmission-electron-microscope-observations-of-free-radical-production-in-human-polymorphonuclear-leukocytes-phagocytosing-non-opsonized-tannerella-forsythia
#1
Keiichi Moriguchi, Yoshiaki Hasegawa, Naoya Higuchi, Yukitaka Murakami, Fuminobu Yoshimura, Kazuhiko Nakata, Masaki Honda
We investigated the association between human polymorphonuclear leukocytes (PMNs) and non-opsonized Tannerella forsythia ATCC 43037 displaying a serum-resistant surface layer (S-layer). When PMNs were mixed with T. forsythia in suspension, the cells phagocytosed T. forsythia cells. Nitro blue tetrazolium (NBT) reduction, indicative of O2- production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H2 O2 production, was observed by electron microscopy. We examined the relationship between high-molecular-weight proteins of the S-layer and Ce reaction (for T...
April 24, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28429380/research-on-micro-damage-behavior-of-wool-fabrics-drying-in-domestic-dryer
#2
Yuhui Wei, Xu Wang, Zhaowei Su, Xuemei Ding
To evaluate the performance degradation of fabric drying in domestic air-vented dryer, the appearance, mechanical, and microscopic properties of the wool fabric after different drying cycles were studied. Pilling and dimensional stability tests were performed according to established international standards. Microscopic studies were carried out by Scanning Electron Microscopy (SEM) and X-ray (XRD). The results show that pilling is the dominant damage mechanism and gradually increased during lower number of drying cycles (0-10 cycles)...
April 21, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28425155/effect-of-platinum-on-sintering-morphology-of-porous-ysz-ceramics
#3
Pan Fang, Junmei Wang, Xian Li, Shijie Wu, Wenlei Zhang, Sufang Li
Platinum/yttria-stabilized zirconia (Pt/YSZ) porous ceramics were prepared by sintering of the Pt/PMMA/YSZ stripe-like membrane with a porogen of spherical polymethyl methacrylate (PMMA) and a precursor of platinum (Pt) of chloroplatinic acid. The microstructure of raw and processed material and Pt/ZrO2 calcination process at different sintering temperatures were investigated. The results showed that the spherically porous structure of Pt/YSZ was observed at a sintering temperature below 400 °C due to the thermal decomposition of PMMA...
April 19, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28401733/effects-of-metals-cadmium-and-chromium-alone-and-in-combination-on-the-liver-and-kidney-tissue-of-male-spraque-dawley-rats-an-ultrastructural-and-electron-energy-loss-spectroscopy-investigation
#4
Chantelle Venter, Hester Magdalena Oberholzer, Franscious Riccardo Cummings, Megan Jean Bester
Heavy metal pollution has increased in the last decades. Water sources are contaminated and human exposure is often long term exposure to variable amounts of different metals. In this study, male Sprague-Dawley rats were exposed via oral gavage for 28 days to cadmium (Cd) and chromium (Cr), alone and in combination at concentrations 1000 times the human World Health Organization's acceptable water limits. Rat equivalent dosages were used. Blood markers of liver and kidney function were measured, changes to cellular morphology was determined with transmission electron microscopy and the intracellular metal localisation was determined with the electron energy-loss spectroscopy and energy filtered transmission electron microscopy analysis...
April 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28401681/effect-of-dentin-pretreatment-with-potassium-oxalate-a-6-year-follow-up
#5
Isabel Cristina Celerino de Moraes Porto, Joatan Lucas de Sousa Gomes Costa, Marcio Rafael Nascimento da Silva Lopes, Johnnatan Duarte de Freitas, Isabelle Lins Macêdo de Oliveira, Gabriela Queiroz de Melo Monteiro, Marcos Antônio Japiassú Resende Montes
Oxalate-based products are effective against dentine sensitivity and have been studied as an option to improve long-term adhesive bonding strength. Our aim was to evaluate the effect of potassium oxalate on the microtensile bond strength (µTBS) of the dentin/resin interface after 24 h, 1, and 6 years. Dentin on the occlusal surface of 16 human premolars was exposed and etched with 35% phosphoric acid. The teeth were divided into four groups. Two groups received 3% monohydrated potassium oxalate and the following adhesive systems and composites: Adper Scotchbond Multipurpose + FiltekZ350 (3M/ESPE) and Prime & Bond NT + Esthet-X (Dentsply)...
April 12, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28379628/nuclear-spatial-and-spectral-features-based-evolutionary-method-for-meningioma-subtypes-classification-in-histopathology
#6
Kiran Fatima, Hammad Majeed, Humayun Irshad
Meningioma subtypes classification is a real-world multiclass problem from the realm of neuropathology. The major challenge in solving this problem is the inherent complexity due to high intra-class variability and low inter-class variation in tissue samples. The development of computational methods to assist pathologists in characterization of these tissue samples would have great diagnostic and prognostic value. In this article, we proposed an optimized evolutionary framework for the classification of benign meningioma into four subtypes...
April 5, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28370895/microscopic-diagnosis-of-the-leaf-and-stem-of-piper-solmsianum-c-dc
#7
A R P Bertocco, I P Migacz, V L P Santos, C R C Franco, R Z Silva, R A Yunes, V Cechinel-Filho, J M Budel
Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1-3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy-dispersive X-ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids)...
March 30, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28370810/the-effects-of-neem-oil-azadirachta-indica-a-juss-enriched-with-different-concentrations-of-azadirachtin-on-the-integument-of-semi-engorged-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae-females
#8
José Ribamar Lima de Souza, Rafael Neodini Remedio, André Arnosti, Rusleyd Maria Magalhães de Abreu, Maria Izabel Camargo-Mathias
Several studies searching for methods to control Rhipicephalus sanguineus s.l., (dog tick) infestations have been developed aiming to minimize the damages caused by these ectoparasites to the hosts and the environment, which is harmed by the indiscriminate use of toxic acaricide products. In this scenario, neem oil has been used as a natural alternative against ticks, once this chemical has repellent properties and interferes in the growth regulation of these ectoparasites, inhibiting ecdysis. The present study evaluated the effects of azadirachtin-enriched neem oil on the integument of semi-engorged R...
March 29, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28370776/texture-analysis-of-parasitological-liver-fibrosis-images
#9
Luminiţa Moraru, Simona Moldovanu, Anisia-Luiza Culea-Florescu, Dorin Bibicu, Amira S Ashour, Nilanjan Dey
Liver fibrosis accurate staging is vital to define the state of the Schistosomiasis disease for further treatment. The present work analyzed the microscopic liver images to identify and to differentiate between healthy, cellular, fibrocellular, and fibrous liver pathologies by proposing a fast, robust, and highly discriminative method based on texture analysis. The multiclass classification based on the "one-versus- all" method that built a voting rule approach to classify the liver images based on the liver state...
March 29, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28370692/quality-evaluation-of-ultra-thin-samples-application-to-graphene
#10
K Cui, K Bosnick, R Indoe, M Malac, R A Mcleod
Many new materials emerging are strictly two dimensional (2D), often only one or two monolayers thick. They include transition metal dichalcogenides, such as MoS2 , and graphene. Graphene in particular appears to have many potential applications. Typically the crystalline film without contamination is of interest. Therefore, a reliable method is needed to routinely evaluate the quality of the synthesized samples. Here, we present one such candidate method that utilizes standard electron diffraction and low/medium magnification imaging in a rudimentary transmission electron microscope...
March 29, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28371124/the-cardiovascular-functions-of-salusin-%C3%AE-mediated-by-muscarinic-receptors-glutamate-receptors-or-l-type-calcium-channels-within-the-rostral-ventrolateral-medulla-of-rats
#11
Fu-Jia Xie, Chen Chai, Ping Zhu, Bin Li, Hong-Yan Cai, Yan Lu, Nong Cao
Salusin-α and salusin-β are newly identified bioactive peptides of 28 and 20 amino acids, respectively, that were initially predicted using in silico analyses and are widely distributed in the endocrine system, hematopoietic system, and central nervous system. The goal of our study was to investigate the cardiovascular effect of salusin-β microinjections into the rostral ventrolateral medulla (RVLM) in anesthetized rats and study their mechanism of action. Microinjection of the artificial cerebrospinal fluid (aCSF) into the RVLM did not affect the blood pressure (BP) or heart rate (HR) in anesthetized rats...
March 28, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28346726/predictive-value-of-p63-ki-67-and-survivin-expression-in-oral-leukoplakia-a-tissue-microarray-study
#12
Kelly Bienk Dias, Anacláudia Pereira Costa Flores, Márcia Gaiger Oliveira, Pantelis Varvaki Rados, Manoel Sant'ana Filho
The aim of this study was to analyze the immunohistochemical expression of survivin, ki-67, and p63 in oral leukoplakic lesions, histopathologically differentiated into dysplastic and nondysplastic. A tissue microarray containing 57 samples of biopsies from clinically classified lesions, such as leukoplakia, was immunolabeled for survivin, ki-67, and p63. Samples were scored for percentage of positively stained. Scores were designated as follows: low = less than 25% of positive cells; and high = more than 25% of positive cells...
March 27, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28322478/comparative-morphology-of-the-leaf-epidermis-in-lobelia-lobelioideae-from-china
#13
Chunjiao Li, Jingmei Lu, Yan Li, Junyi Zhu, Lifan Zhang
The leaf epidermal morphology of 38 samples, representing 22 Lobelia species from China, was studied using light microscopy and scanning electron microscopy. The stomata and other epidermal features were largely consistent within species, and therefore represented good characters for taxonomic purposes. Diagnostic characters of the leaf epidermis were used to differentiate species of Lobelia. The species pairs L. clavata and L. pyramidalis, L. alsinoides and L. terminalis, and L. davidii and L. erectiuscula, were differentiated using leaf epidermal characters...
March 21, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28304124/distinguishing-the-chinese-materia-medica-tiepishihu-from-similar-dendrobium-species-of-the-same-genus-using-histological-and-microscopic-method
#14
Kun-Zi Yu, Hua Yan, Hai-Chuan Tai, Nan-Ping Zhang, Xian-Long Cheng, Zeng-Xi Guo, Shuang-Cheng Ma, Feng Wei
The Chinese Materia Medica, Tiepishihu, used as a tonic for over one thousand years, is a well-known precious medicine in China. According to the Chinese Pharmacopoeia, its source is the species Dendrobium officinale Kimura et Migo, which is distinguished from other species in Dendrobium genus. However, these species from the same genus are similar with Tiepishihu and caused confusion in the market. To find a quick and simple method to distinguish Tiepishihu from other similar species, histologic and microscopic methods were combined together to investigate the transverse section of stem of Tiepishihu and other similar species...
March 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28295852/improved-capacity-to-evaluate-changes-in-intestinal-mucosal-surface-area-using-mathematical-modeling
#15
Chasen J Greig, Robert A Cowles
Quantification of intestinal mucosal growth typically relies on morphometric parameters, commonly villus height, as a surrogate for presumed changes in mucosal surface area (MSA). We hypothesized that using mathematical modeling based on multiple unique measurements would improve discrimination of the effects of interventions on MSA compared to standard measures. To determine the ability of mathematical modeling to resolve differences in MSA, a mouse model with enhanced serotonin (5HT) signaling known to stimulate mucosal growth was used...
March 13, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28294460/retinal-imaging-analysis-based-on-vessel-detection
#16
Arshad Jamal, Mohammed Hazim Alkawaz, Amjad Rehman, Tanzila Saba
With an increase in the advancement of digital imaging and computing power, computationally intelligent technologies are in high demand to be used in ophthalmology cure and treatment. In current research, Retina Image Analysis (RIA) is developed for optometrist at Eye Care Center in Management and Science University. This research aims to analyze the retina through vessel detection. The RIA assists in the analysis of the retinal images and specialists are served with various options like saving, processing and analyzing retinal images through its advanced interface layout...
March 13, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28295892/macroanatomic-light-and-electron-microscopic-examination-of-pecten-oculi-in-the-seagull-larus-canus
#17
Nazan Gezer Ince, Burcu Onuk, Yonca Betil Kabak, Aydin Alan, Murat Kabak
The present study was conducted to determine macroanatomic characteristic as well as light and electron microscopic examination (SEM) of pecten oculi and totally 20 bulbus oculi belonging to 10 seagulls (Larus canus) were used. Pecten oculi formations consisted of 18 to 21 pleats and their shape looked like a snail. Apical length of the pleats forming pecten oculi were averagely measured as 5.77 ± 0.56 mm, retina-dependent base length was 9.01 ± 1.35 mm and height was measured as 6.4 ± 0.62 mm...
March 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28295841/morphogenesis-and-developmental-ultrastructure-of-nicotiana-tabacum-short-glandular-trichomes
#18
Branka Uzelac, Dušica Janošević, Dragana Stojičić, Snežana Budimir
The anatomy and ultrastructure of the short glandular trichomes occurring on young expanding leaves of Nicotiana tabacum were investigated using light and transmission electron microscopy. The objective of the present research was to characterize the cellular changes that occur during morphogenesis of short glandular trichomes, from initiation to senescence. Ultrastructural analysis of their secretory cells revealed characteristics common to gland cells: numerous mitochondria in highly organized cytoplasm, large nuclei, and an elaborate network of endoplasmic reticulum...
March 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28271628/zinc-oxide-3d-microstructures-as-an-antimicrobial-filler-content-for-composite-resins
#19
Hércules Bezerra Dias, Maria Inês Basso Bernardi, Matheus Aparecido Dos Santos Ramos, Tamara Carolina Trevisan, Taís Maria Bauab, Antônio Carlos Hernandes, Alessandra Nara de Souza Rastelli
The aim of this study was to evaluate the antibacterial activity of a composite resin modified by 3D zinc oxide (ZnO) microstructures and to verify possible alterations on its mechanical properties. ZnO was synthesized by hydrothermal approach and characterized by X-ray diffraction (XRD), surface area by Brunauer, Emmett and Teller (BET), Fourier transform infrared spectroscopy (FTIR) and Field emission scanning electron microscopy (FESEM). The minimum inhibitory concentrations of ZnO against Streptococcus mutans, Escherichia coli, Staphylococcus aureus, and Candida albicans were determinated...
March 8, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28271594/sealing-ability-and-adaptation-of-root-end-filling-materials-in-cavities-prepared-with-different-techniques
#20
Selen Küçükkaya Eren, Mehmet Ömer Görduysus, Cem Şahin
The aim of this study was to evaluate the sealing ability and marginal adaptation of calcium silicate-based cements (CSCs) in root-end cavities prepared by ultrasonic and laser tips. A total of 72 extracted human maxillary incisor teeth were randomly divided as 60 teeth in experimental groups and 6 teeth each for positive and negative control groups. Specimens in experimental groups were obturated, their root-end resections were performed and randomly divided into six groups (n = 10) as follows: G1: Ultrasonic retrotip + MTA, G2: Ultrasonic retrotip + Calcium Enriched Mixture (CEM), G3: Ultrasonic retrotip + Biodentine, G4: Er:YAG laser tip + MTA, G5: Er:YAG laser tip + CEM, G6: Er:YAG laser tip + Biodentine...
March 8, 2017: Microscopy Research and Technique
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