journal
MENU ▼
Read by QxMD icon Read
search

Developmental Dynamics: An Official Publication of the American Association of Anatomists

journal
https://www.readbyqxmd.com/read/30106495/effective-differentiation-of-induced-pluripotent-stem-cells-into-dental-cells
#1
Eun-Jung Kim, Kyung-Sik Yoon, Makiko Arakaki, Keishi Otsu, Satoshi Fukumoto, Hidemitsu Harada, David William Green, Jong-Min Lee, Han-Sung Jung
BACKGROUND: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use iPSCs. However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. RESULTS: Here, we tried to induce differentiation methods for dental epithelial cells and dental mesenchymal cells from iPSCs...
August 14, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30091290/applications-of-human-brain-organoids-to-clinical-problems
#2
REVIEW
H Isaac Chen, Hongjun Song, Guo-Li Ming
Brain organoids are an exciting new technology with the potential to significantly change how diseases of the brain are understood and treated. These three-dimensional neural tissues are derived from the self-organization of pluripotent stem cells, and they recapitulate the developmental process of the human brain, including progenitor zones and rudimentary cortical layers. Brain organoids have been valuable in investigating different aspects of developmental neurobiology and comparative biology. Several characteristics of organoids also make them attractive as models of brain disorders...
August 9, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30079980/mmp14-regulates-cranial-neural-crest-epithelial-to-mesenchymal-transition-and-migration
#3
Taylor Garmon, Megen Wittling, Shuyi Nie
BACKGROUNDS: Neural crest is a vertebrate specific cell population. Induced at lateral borders of the neural plate, neural crest cells (NCCs) subsequently undergo epithelial-to-mesenchymal transition (EMT) to detach from the neuroepithelium before migrating into various locations in the embryo. Despite the wealth of knowledge of transcription factors involved in this process, little is known about the effectors that directly regulate neural crest EMT and migration. RESULTS: Here, we examined the activity of matrix metalloproteinase MMP14 in NCCs and found that MMP14 is expressed in both premigratory and migrating NCCs...
August 6, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30067309/promoting-remyelination-through-cell-transplantation-therapies-in-a-model-of-viral-induced-neurodegenerative-disease
#4
REVIEW
Vrushali Mangale, Laura L McIntyre, Craig M Walsh, Jeanne F Loring, Thomas E Lane
Multiple sclerosis (MS) is a central nervous system (CNS) disease characterized by chronic neuroinflammation, demyelination, and axonal damage. Infiltration of activated lymphocytes and myeloid cells are thought to be primarily responsible for white matter damage and axonopathy. A number of FDA-approved therapies exist that impede activated lymphocytes from entering the CNS thereby limiting new lesion formation in patients with relapse-remitting forms of MS (RRMS). However, a significant challenge within the field of MS research is to develop effective and sustained therapies that allow for axonal protection and remyelination...
August 1, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30055071/nosip-functions-during-vertebrate-eye-and-cranial-cartilage-development
#5
Hannah Flach, Julia Krieg, Meike Hoffmeister, Petra Dietmann, Adrian Reusch, Lisa Wischmann, Bianka Kernl, Ricarda Riegger, Stefanie Oess, Susanne J Kühl
BACKGROUND: The nitric oxide synthase interacting protein (Nosip) has been associated with diverse human diseases including psychological disorders. In line, early neurogenesis of mouse and Xenopus is impaired upon Nosip deficiency. Nosip knock out mice show craniofacial defects and the down-regulation of Nosip in the mouse and Xenopus leads to microcephaly. Until now, the exact underlying molecular mechanisms of these malformations are still unknown. RESULTS: Here, we show that nosip is expressed in the developing ocular system as well as the anterior neural crest cells of Xenopus laevis...
July 28, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30055068/effect-of-shrna-mediated-xist-knockdown-on-the-quality-of-porcine-parthenogenetic-embryos
#6
Xiaoyu Chen, Zhiwei Zhu, Fuxian Yu, Jing Huang, Ruoxin Jia, Jianzhi Pan
BACKGROUND: Parthenogenetically activated oocytes exhibit poor embryo development and lower total numbers of cells per blastocyst accompanied by abnormally increased expression of Xist, a long non-coding RNA that plays an important role in triggering X chromosome inactivation during embryogenesis. RESULTS: To investigate whether knockdown of Xist influences parthenogenetic development in pigs. We developed an anti-Xist short hairpin RNA (shRNA) vector, which can significantly inhibit Xist expression for at least seven days when injected at 12-13 hours after parthenogenetic activation...
July 28, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30016584/concepts-towards-directing-human-astroplasticity-to-promote-neuroregeneration
#7
REVIEW
Rajan Patel, Matthew Muir, Caroline Cvetkovic, Robert Krencik
Astrocytes exhibit dynamic and complex reactions to various insults. Recently, investigations into the transitions that occur during cellular specification, differentiation, maturation and disease responses have provided insights into understanding the mechanisms that underlie these altered states of reactivity and function. Here we summarize current concepts in how astrocyte state transitions, termed 'astroplasticity', are regulated and also how this affects neural circuit function through extracellular signaling...
July 17, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30016580/nck2-is-essential-for-limb-trajectory-selection-by-spinal-motor-axons
#8
Chih-Ju Chang, Ming-Yuan Chang, Yi-Chao Lee, Kai-Yun Chen, Tsung-I Hsu, Yi-Hsin Wu, Jian-Ying Chuang, Tzu-Jen Kao
BACKGROUND: The development of a functioning nervous system requires precise assembly of neuronal connections, which can be achieved by the guidance of axonal growth cones to their proper targets. How axons are guided by signals transmitted to the cytoskeleton through cell surface-expressed guidance receptors remains unclear. We investigated the function of Nck2 adaptor protein as an essential guidance intermediary in the context of spinal lateral motor column (LMC) motor axon trajectory into the limb...
July 17, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/30016568/a-wilson-cell-origin-for-kupffer-s-vesicle-in-the-zebrafish
#9
Rachel M Warga, Donald A Kane
BACKGROUND: Bilaterally symmetric animals have evolved highly reproducible asymmetries between left and right. In teleosts, Kupffer's Vesicle, the structure necessary for the determination of left-right asymmetry, is derived from a group of cells in the gastrula termed the dorsal forerunners. RESULTS: "Wilson cells" are a ring of marginal enveloping layer cells that are cytoplasmically connected to the yolk cell and thus the last blastomeres to inherit yolk cell cytoplasm...
July 17, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29920846/intercalated-cushion-cells-within-the-cardiac-outflow-tract-are-derived-from-the-myocardial-troponin-t-type-2-tnnt2-cre-lineage
#10
Joshua J Mifflin, Loren E Dupuis, Nicolas E Alcala, Lea G Russell, Christine B Kern
BACKGROUND: The origin of the intercalated cushions that develop into the anterior cusp of the pulmonary valve (PV) and the noncoronary cusp of the aortic valve (AV) is not well understood. RESULTS: Cre transgenes in combination with the Rosa TdTomato-EGFP reporter were used to generate three-dimensional lineage mapping of AV and PV cusps during intercalated cushion development. Tie2-Cre;EGFP was used to mark endothelial-derived mesenchymal cells, Wnt1-Cre;EGFP for cardiac neural crest and cardiac Troponin T (Tnnt2)Cre;EGFP, for myocardial lineage...
June 19, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29806219/alterations-in-retinoic-acid-signaling-affect-the-development-of-the-mouse-coronary-vasculature
#11
Suya Wang, Weiliang Huang, Hozana A Castillo, Maureen A Kane, José Xavier-Neto, Paul A Trainor, Alexander R Moise
BACKGROUND: During the final stages of heart development the myocardium grows and becomes vascularized via paracrine factors and cell progenitors derived from the epicardium. There is evidence to suggest that retinoic acid (RA), a metabolite of vitamin A, plays an important role in epicardial-based developmental programming. However, the consequences of altered RA-signaling in coronary development have not been systematically investigated. RESULTS: We explored the developmental consequences of altered RA-signaling in late cardiogenic events that involve the epicardium...
May 27, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29806169/a-transient-window-of-resilience-during-early-development-minimizes-teratogenic-effects-of-heat-in-zebrafish-embryos
#12
Triveni Menon, Sreelaja Nair
Background Transient heat shock during early development is an established experimental paradigm for doubling the genome of the zebrafish zygote, which has practical applications in expedited identification of recessive mutations in genetic screens. Despite the simplicity of the strategy and the genetic tractability of zebrafish, heat shock has not been used for genome doubling since the proof-of-principle experiments done in the 1980s. This is because of poor survival of embryos that ensue from transient heat shocks and gross developmental abnormalities in the few survivors, which is incompatible with phenotype driven screens...
May 27, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29665133/isolation-of-a-unique-hepatic-stellate-cell-population-expressing-integrin-%C3%AE-8-from-embryonic-mouse-livers
#13
Tomohiro Ogawa, Yuchang Li, Ingrid Lua, Andrea Hartner, Kinji Asahina
BACKGROUND: Hepatic stellate cells (HSCs) play an important role in liver fibrogenesis. However, little is known about their phenotype and role in liver development. The aim of this study is to identify specific markers for embryonic HSCs. RESULTS: Using antibodies against ALCAM and PDPN, we separated mesothelial cells (MCs) and HSCs from developing livers and identified integrin α8 (ITGA8) as a marker for embryonic desmin+ HSCs that are preferentially localized near the developing liver surface and α-smooth muscle actin+ perivascular mesenchymal cells around the vein...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29633426/calcium-signals-act-through-histone-deacetylase-to-mediate-pronephric-kidney-morphogenesis
#14
Sarah C Rothschild, Hunter J Lee, Sarah R Ingram, Daniel K Mohammadi, Gregory S Walsh, Robert M Tombes
BACKGROUND: Autosomal dominant polycystic kidney disease is the most common monogenetic kidney disorder and is linked to mutations in PKD1 and PKD2. PKD2, a Ca2+ -conducting TRP channel enriched in ciliated cells and gated by extracellular signals, is necessary to activate the multifunctional Ca2+/ calmodulin-dependent protein kinase type 2 (CaMK-II), enabling kidney morphogenesis and cilia stability. RESULTS: In this study, antisense morpholino oligonucleotides and pharmacological compounds were employed to investigate the roles of class II HDAC family members (HDAC 4, 5, and 6) in Zebrafish kidney development...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29569346/evolution-of-caudal-fin-ray-development-and-caudal-fin-hypural-diastema-complex-in-spotted-gar-teleosts-and-other-neopterygian-fishes
#15
Thomas Desvignes, Andrew Carey, John H Postlethwait
BACKGROUND: The caudal fin of actinopterygians transitioned from a heterocercal dorsoventrally asymmetrical fin to a homocercal externally symmetrical fin in teleosts through poorly understood evolutionary developmental mechanisms. We studied the caudal skeleton of major living actinopterygian lineages, including polypteriformes, acipenseriformes, Holostei (gars and bowfin), and teleosts, compared with reports of extinct neopterygians and basal teleosteans. We focused on the hypural diastema complex, which includes (1) a gap between hypurals 2 and 3, that (2) separates two plates of connective tissue at (3) the branching of caudal vasculature; these features had been considered as a shared, derived trait of teleosts, a synapomorphy...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29532607/hippo-yap-signaling-in-ocular-development-and-disease
#16
REVIEW
Matthew Lee, Navneet Goraya, Seonhee Kim, Seo-Hee Cho
The Hippo-Yes associated protein (Yap) pathway plays an important role in organ size control by regulating cell proliferation, apoptosis, and stem cell renewal. Hippo-Yap signaling also functions at the level of cellular development in a variety of organs through its effects on cell cycle control, cell survival, cell polarity, and cell fate. Because of its important roles in normal development and homeostasis, abnormal regulation of this pathway has been shown to lead to pathological outcomes such as tissue overgrowth, tumor formation, and abnormal organogenesis, including ocular-specific disorders...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29532564/etv5-regulates-ductal-morphogenesis-with-sox9-and-is-critical-for-regeneration-from-pancreatitis
#17
Koushik K Das, Steffen Heeg, Jason R Pitarresi, Maximilian Reichert, Basil Bakir, Shigetsugu Takano, Janel L Kopp, Anja Wahl-Feuerstein, Philip Hicks, Maike Sander, Anil K Rustgi
BACKGROUND: The plasticity of pancreatic acinar cells to undergo acinar to ductal metaplasia (ADM) has been demonstrated to contribute to the regeneration of the pancreas in response to injury. Sox9 is critical for ductal cell fate and important in the formation of ADM, most likely in concert with a complex hierarchy of, as yet, not fully elucidated transcription factors. RESULTS: By using a mouse model of acute pancreatitis and three dimensional organoid culture of primary pancreatic ductal cells, we herein characterize the Ets-transcription factor Etv5 as a pivotal regulator of ductal cell identity and ADM that acts upstream of Sox9 and is essential for Sox9 expression in ADM...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29532549/a-novel-role-for-cilia-dependent-sonic-hedgehog-signaling-during-submandibular-gland-development
#18
Kelsey H Elliott, Grethel Millington, Samantha A Brugmann
BACKGROUND: Submandibular glands (SMGs) are specialized epithelial structures which generate saliva necessary for mastication and digestion. Loss of SMGs can lead to inflammation, oral lesions, fungal infections, problems with chewing/swallowing, and tooth decay. Understanding the development of the SMG is important for developing therapeutic options for patients with impaired SMG function. Recent studies have suggested Sonic hedgehog (Shh) signaling in the epithelium plays an integral role in SMG development; however, the mechanism by which Shh influences gland development remains nebulous...
June 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29770538/integral-bhlh-factor-regulation-of-cell-cycle-exit-and-rgc-differentiation
#19
Kate A Maurer, Angelica Kowalchuk, Farnaz Shoja-Taheri, Nadean L Brown
BACKGROUND: In the developing mouse embryo, the bHLH transcription factor Neurog2 is transiently expressed by retinal progenitor cells and required for the initial wave of neurogenesis. Remarkably, another bHLH factor, Ascl1, normally not present in the embryonic Neurog2 retinal lineage, can rescue the temporal phenotypes of Neurog2 mutants. RESULTS: Here we show that Neurog2 simultaneously promotes terminal cell cycle exit and retinal ganglion cell differentiation, using mitotic window labeling and integrating these results with retinal marker quantifications...
May 17, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/29770532/novel-approaches-to-study-coronary-vasculature-development-in-mice
#20
Hana Kolesová, Martin Bartoš, Wan Chin Hsieh, Veronika Olejníčková, David Sedmera
BACKGROUND: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. RESULTS: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development...
May 17, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
journal
journal
30931
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"