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Journal of Fluorescence

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https://www.readbyqxmd.com/read/30022376/thiol-capped-gold-nanoparticle-biosensors-for-rapid-and-sensitive-visual-colorimetric-detection-of-klebsiella-pneumoniae
#1
Sepideh Ahmadi, Hossein Kamaladini, Fatemeh Haddadi, Mohammad Reza Sharifmoghadam
In the last few years, gold nanoparticle biosensors have been developed for rapid, precise, easy and inexpensive with high specificity and sensitivity detection of human, plant and animal pathogens. Klebsiella pneumoniae serotype K2 is one of the common gram-negative pathogens with high prevalence. Therefore, it is essential to provide the effective and exclusive method to detect the bacteria. Klebsiella pneumoniae serotype K2 strain ATCC9997 genomic DNA was applied to establish the detection protocol either with thiol-capped oligonucleotide probes and gold nanoparticles or polymerase chain reaction based on K2A gene sequence...
July 18, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/30014275/synthesis-photophysical-properties-and-application-of-new-porphyrin-derivatives-for-use-in-photodynamic-therapy-and-cell-imaging
#2
Prasad G Mahajan, Nilam C Dige, Balasaheb D Vanjare, Abdul Rehman Phull, Song Ja Kim, Seong-Karp Hong, Ki Hwan Lee
New derivatives of tetrakis(4-carboxyphenyl) porphyrin were designed, synthesized and characterized by IR, proton NMR and mass spectroscopy. The ground and excited state nature of new derivatives were examined using UV-Vis. absorption and fluorescence spectroscopy, fluorescence quantum yield and fluorescence lifetime studies. The singlet oxygen quantum yield of each synthesized derivative of porphyrin was estimated for their further efficacy as potential photosensitizer in biological studies. The significant photophysical data of all synthesized derivatives was supplementary accessed to examine the cell imaging and cytotoxicity against two cancer cell lines viz...
July 16, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/30014274/green-emitting-cerium-doped-cas-whiskers-grown-by-solid-state-diffusion-method
#3
S Rekha, E I Anila
Undoped and cerium doped Calcium sulfide (CaS) phosphors were synthesized using solid state diffusion method. The X-ray diffraction pattern revealed that both undoped and doped CaS crystallites have cubic structure with average crystallite size varying from 20 to 30 nm. Scanning electron micrographs indicated that Ce doped CaS phosphors were composed of whiskers with different dimensions and orientations. The optical properties of undoped and Ce doped particles were characterized using Photoluminescence (PL) and UV-Vis absorption spectroscopy...
July 16, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/30008060/lipid-bilayer-interactions-of-amyloidogenic-n-terminal-fragment-of-apolipoprotein-a-i-probed-by-f%C3%A3-rster-resonance-energy-transfer-and-molecular-dynamics-simulations
#4
Galyna P Gorbenko, Valeriya Trusova, Chiharu Mizuguchi, Hiroyuki Saito
The effects of one of the amyloidogenic mutations of apolipoprotein A-I (apoA-I), G26R, on the thermal stability, structural dynamics and lipid-associating properties of the 1-83 N-terminal fragment of apoA-I (A83) have been investigated using the Förster resonance energy transfer (FRET) and molecular dynamics (MD) simulation. The measurements of FRET between the tryptophan residues of the single Trp variants of A83 as donors and the membrane-incorporated fluorescent probe 4-dimethylaminochalcone as an acceptor provided evidence for a less depth of A83/G26R penetration into phosphatidylcholine (PC) bilayer compared to WT counterpart...
July 15, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/30008059/review-on-recent-advances-in-metal-ions-sensing-using-different-fluorescent-probes
#5
REVIEW
Suman Chowdhury, Bipin Rooj, Ankita Dutta, Ujjwal Mandal
Fluorescence probes serves as unique detection methods for its simplicity and low detection limit (LOD) and especially bioimaging ability. Research on the probes has already sprouted during the last decade with the help of its molecular recognition properties. This review spotlights recent progress in sensing and bioimaging biologically, environmentally and industrially important metal ions e.g. Zn2+ , Cu2+ , Hg2+ , Ag+ etc. using suitable fluorescent chemosensors including carbon quantum dots (CQD).
July 14, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29992458/a-novel-fluorescent-nanoparticle-for-sensitive-detection-of-cry1ab-protein-in-vitro-and-in-vivo
#6
Xiao Xu, Hao Chen, Yuancheng Cao, Yongjun Lin, Jun'an Liu
Here, we report the synthesis and characterization of CoFe2 O4 doping Ag2 S dendrimer-modified nanoparticles (CoFe2 O4 -Ag2 S DMNs) in Cry1Ab protein detection and imaging. The near-infrared Ag2 S quantum dots were first prepared by using the thermal decomposition method, followed by modification of the water-soluble quantum dots using the method of solvent evaporation and ligand exchange, and finally the fluorescent magnetic bifunctional nanoparticles were obtained by binding with CoFe2 O4 . As-prepared CoFe2 O4 -Ag2 S DMNs were characterized by fluorescence (FL) spectroscopy and transmission electron microscopy (TEM)...
July 10, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29974315/a-micelle-enhanced-spectrofluorimetric-determination-of-pirfenidone-application-to-content-uniformity-testing-and-human-urine
#7
Naga Gayatri Sambhani, Valsala Madhavan Nair Biju
The present manuscript gives a detailed account of highly selective, validated and sensitive method for quantification of pirfenidone in its pharmaceutical dosage forms and spiked human urine. The developed method is relied on the systematic study of the fluorescence action of Pirfenidone in Tween - 80 micellar medium. The Pirfenidone exhibits strong fluorescence at λem 396 nm upon excitation at λex 318 nm in Tween -80 medium. The fluorescence - concentration plot was linear over concentration range of 0...
July 4, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29968047/quick-microwave-assisted-synthesis-and-in-vitro-imaging-application-of-oxygen-doped-fluorescent-carbon-dots
#8
Raja Angamuthu, Ramesh Rajendran, Raj Vairamuthu
In this paper, a fast and simplest one-pot tactic was used to synthesis fluorescent oxygen doped carbon dots from Tween-20 (TTO-CDs) is reported. The TTO-CDs were microwavically synthesized by using Tween-20 as both the carbon precursor and the oxygen dopant as well. The surface morphology, crystalline and/or amorphous nature, composition and optical assets of synthesized TTO-CDs were studied by means of existing techniques. From the results, it was confirmed that the as-synthesized TTO-CDs are amorphous in nature, monodispersed, sphere-shaped and the typical particle size range is 5 ± 1...
July 2, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29961205/spectroscopic-computational-antimicrobial-dna-interaction-in-vitro-anticancer-and-molecular-docking-properties-of-biochemically-active-cu-ii-and-zn-ii-complexes-of-pyrimidine-ligand
#9
Murugesan Sankarganesh, Jeyaraj Dhaveethu Raja, Paul Raj Adwin Jose, Gujuluva Gangatharan Vinoth Kumar, Jegathalaprathaban Rajesh, Ramalingam Rajasekaran
Biochemically active Cu(II) and Zn(II) complexes [CuL(ClO4 )2 (1) and ZnL(ClO4 )2 (2)] have been synthesized from N,N donor Schiff base ligand L derived from4,6-dichloropyrimdine-5-carboxaldehyde with 4-(2-aminoethyl)morpholine. The L, complexes 1 and 2 have been structurally characterized by elemental analysis, 1 H-NMR, FTIR, MS, UV-Visible and ESR techniques. The results obtained from the spectral studies supports the complexes 1 and 2 are coordinated with L through square planar geometry. DFT calculations results supports, the ligand to metal charge transfer mechanism can occur between L and metal(II) ions...
June 30, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29961204/the-time-resolved-fluorescence-stokes-shift-of-cucurbit-6-uril-complexes-with-a-pyridinium-styryl-dye
#10
N Kh Petrov, D A Ivanov, I V Kryukov, A D Svirida, Yu A Shandarov, M V Alfimov
The time-resolved Stokes shift of fluorescence of styryl dye, trans-4-[4-(dimethylamino)-styryl]-1-methylpyridinium iodide (DASPI), and its host-guest complexes with cucurbit[6]uril (CB[6]) was studied by up-conversion technique on a 5 ps time scale. In all cases, the solvation time correlation function - that describes relaxation of the fluorescent state - contains two components. The time constant of the first component, which is related to the vibrational relaxation of the dye cation, increases from 118 fs without CB[6] to 224 fs in the presence CB[6]...
June 30, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29961203/excimer-emission-of-acridine-orange-adsorbed-on-gadolinium-yttrium-orthovanadate-nanoparticles
#11
K O Hubenko, S L Yefimova, T N Tkacheva, P O Maksimchuk, O O Sedyh, O G Viagin, A V Sorokin, Yu V Malyukin
Studying the complexes of inorganic nanoparticles - organic dye molecules is of great importance for their theranostics application. In this paper, we report gadolinium-yttrium orthovanadate nanoparticles (VNPs) - Acridine Orange (AO) complex formation in water solutions. To study the interactions between VNPs and AO, the methods of steady-state and time-resolved spectroscopy were used. It was shown that in aqueous solutions containing VNPs, AO aggregation takes place with a sandwich-like stacking of AO molecules in the near-surface layer of VNPs...
June 30, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29961202/singlet-oxygen-generating-properties-of-different-sizes-of-charged-graphene-quantum-dot-nanoconjugates-with-a-positively-charged-phthalocyanine
#12
Refilwe Matshitse, Tebello Nyokong
Various sizes of graphene quantum dots (GQDs) denoted as GQD2 , GQD6 and GQD10 (increasing in size) were non-covalently attached to 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]-phthalocyanine (ZnTPPcQ) to form GQDs-ZnTPPcQ nanoconjugates. X-ray photoelectron spectroscopy (XPS) showed that increasing sizes of GQDs decreases the atomic concentrations of oxygen, which leads to blue shift in spectra of the GQDs. Relative to Pcs alone (0.03), the presence of GQDs improved the singlet oxygen quantum yields with the following values: GQD2 -ZnTPPcQ (0...
June 30, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29959579/comparative-dft-computational-studies-with-experimental-investigations-for-novel-synthesized-fluorescent-pyrazoline-derivatives
#13
Ahmad Saed Salim, Adel S Girgis, Altaf H Basta, Houssni El-Saied, Mohamed A Mohamed, Ahmad H Bedair
A novel series of pyrazoline derivatives were synthesized and their spectral properties were characterized via FT-IR, 1 H, and 13 C NMR. The electronic transitions and fluorescence properties were tracked via UV-Vis and emission spectrometry. The density functional theory (DFT) calculations have been also computed to get spot onto the geometry, electronic transitions and spectroscopic properties theoretically that has been compared with the encountered experimental ones. Moreover, the dipole moment, optimized energy, HOMO - LUMO energies and band gaps were calculated for novel candidates pyrazoline derivatives with highly fluorescence quantum yield...
June 29, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29959578/aggregation-behavior-of-phlip-in-aqueous-solution-at-low-concentrations-a-fluorescence-study
#14
Bhagyashree D Rao, Hirak Chakraborty, Sandro Keller, Amitabha Chattopadhyay
pH (low) insertion peptide (pHLIP) is a 36-residue peptide derived from the third transmembrane helix of the membrane protein bacteriorhodopsin. The hydrophobicity of this peptide makes it prone to aggregation even at low concentrations, but this has not been studied in detail. In this work, we characterized monomeric and aggregated forms of pHLIP in aqueous solution (pH 8) at low concentrations (~μM) using fluorescence-based approaches, complemented by circular dichroism (CD) spectroscopy. We show here that monomeric and aggregated pHLIP display differential red edge excitation shift (REES) and CD spectra...
June 29, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29951715/enhanced-exciplex-emission-of-pyrene-thin-films-doped-by-perylene-structural-photophysical-and-morphological-investigation
#15
Netaji K Desai, Prasad G Mahajan, Sawanta S Mali, Govind B Kolekar, Shivajirao R Patil
The present work reports on the preparation of thin films of pyrene luminophors doped by varying amounts of perylene by spin coating technique. The structural, morphological, and photophysical properties of pyrene thin films have been investigated as a function of perylene contents. X-ray diffraction studies of doped thin films show well-defined peaks, and estimated crystallite size decreases with increasing perylene content, due to the formation of closed packed crystal structure. SEM images of pure pyrene revealed smooth, and compact and separated crystals with amounts of perylene...
June 27, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29943211/physicochemical-and-antioxidant-properties-of-riboflavin-in-dextran70-hsa-systems
#16
Mariana Voicescu, Sorana Ionescu, Cecilia Lete
Physicochemical properties of Riboflavin (Vitamin B2 ) (RF), in Dextran 70 (Dx70) (a biological relevant glucidic type macromolecule) and Human Serum Albumin (HSA) (a carrier/transport protein) based system, have been studied by absorption, fluorescence, circular dichroism and electrochemistry. No significant changes on the fluorescence of RF in Dx70/HSA systems with and without the influence of temperature (30-60 °C range) were observed. No changes on the intrinsic Tryptophan fluorescence in Dx70/RF/HSA system, have been evidenced...
June 26, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29938390/two-photon-spectroscopy-can-serve-as-a-marker-of-protein-denaturation-pathway
#17
Dipak Kumar Das, Sk Imadul Islam, Nirnay Samanta, Yogendra Yadav, Debabrata Goswami, Rajib Kumar Mitra
Rhodamine group of molecules are widely used dyes for imaging of biological molecules. Application of these dyes however includes a limitation that these molecules absorb in the visible range of the spectrum, which does not fall in the 'biologically transparent window' (BTW). Two photon absorption (TPA) process could come up with an alternate solution to this as these dyes could be excited in the near infrared (NIR) window to extract similar information. To validate this we have investigated TPA cross section (TPACS, σ2 ) of two rhodamine dyes, namely Rhodamine 6G (R6G), Rhodamine B (RhB), site selectively bound with a model protein, bovine serum albumin (BSA), by exciting at 800 nm...
June 25, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29938389/a-bodipy-based-fluorescent-probe-for-the-rapid-detection-of-hypochlorite
#18
Lusheng Wang, Bolin Li, Chao Jiang, Ruopei Sun, Peizhuo Hu, Suwen Chen, Wangsuo Wu
A new boron-dipyrromethene (BODIPY) fluorescent dye aimed at sensitively detecting hypochlorite anion (ClO- ) has been designed, synthesized and characterized. The probe is comprised of a BODIPY fluorophore unit and a ClO- specific reactive group of amidoxime. The addition of hypochlorite results in a red-shift of absorption and emission spectra of the probe accompanied by a decrease of intensity and spectra changes (A500 and 1/I512 ) of the probe can achieve a good linearity to the concentration of ClO- . The fluorescence probe can react to ClO- rapidly (within 60 s) in a wide pH range (4-10) with high sensitivity (detection limit of 6...
June 25, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29936595/a-colorimetric-and-fluorescent-indicator-for-hg-2-detection-based-on-cinnamamide-group-containing-rhodamine-derivative
#19
Zhenglong Yang, Kangyu Fu, Jing Yu, Peiting Zhou, Zhihao Cheng
A colorimetric and fluorescent indicator based on cinnamamide group-containing rhodamine derivative was synthesized for the detection of Hg2+ . The rhodamine B and cinnamamide were connected via ethylenediamine as a bridging molecule through a condensation reaction to obtain a colorimetric and fluorescent indicator for the detection of Hg2+ in H2 O-EtOH (4:1, v/v). The indicator was excellent in the selectivity of Hg2+ and was almost unaffected by other common ions such as Na+ , K+ , Mg2+ , Fe3+ , Cu2+ , Zn2+ , Cr3+ ...
June 24, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29934744/multivariate-curve-resolution-alternate-least-square-analysis-of-excitation-emission-matrices-for-maize-flour-contaminated-with-aflatoxin-b1
#20
Dragana Bartolić, Mira Stanković, Dragosav Mutavdžić, Slavica Stanković, Dragoljub Jovanović, Ksenija Radotić
In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing...
June 22, 2018: Journal of Fluorescence
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