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Phytochemical Analysis: PCA

Saidu Jibril, Norazah Basar, Hasnah Mohd Sirat, Roswanira Abdul Wahab, Naji Arafat Mahat, Lutfun Nahar, Satyajit D Sarker
INTRODUCTION: Cassia singueana Del. (Fabaceae) is a rare medicinal plant used in the traditional medicine preparations to treat various ailments. The root of C. singueana is a rich source of anthraquinones that possess anticancer, antibacterial and antifungal properties. OBJECTIVE: The objective of this study was to develop an ultrasound-assisted extraction (UAE) method for achieving a high extraction yield of anthraquinones using the response surface methodology (RSM), Box-Behnken design (BBD), and a recycling preparative high-performance liquid chromatography (HPLC) protocol for isolation of anthraquinones from C...
October 4, 2018: Phytochemical Analysis: PCA
Lucy Owen, Alex W White, Katie Laird
INTRODUCTION: The antimicrobial activity of many essential oils (EOs) is well established, indicating that EOs may be a source of compounds for antimicrobial drug development. Thin layer chromatography-direct bioautography (TLC-DB) can quickly identify antimicrobial components in complex mixtures and can be applied to the screening of EOs for lead compounds. OBJECTIVES: This study aimed to identify antimicrobial components of oregano, rosewood and cumin EOs against antibiotic-sensitive and -resistant bacteria using TLC-DB and a multi-faceted approach of GC-MS, LC-MS and NMR techniques to characterise bioactive compounds...
October 2, 2018: Phytochemical Analysis: PCA
Carlos Rial, Rosa M Varela, José M G Molinillo, Juan A López-Ráez, Francisco A Macías
INTRODUCTION: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10-10  M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical methods with very low limits of quantification. OBJECTIVE: To develop a sensitive and validated analytical method for the simultaneous quantification of seven SLs [7-oxoorobanchyl acetate (1), solanacol (2), orobanchol (4), strigol (5), fabacyl acetate (6), orobanchyl acetate (7), and 5-deoxystrigol (8)]...
October 2, 2018: Phytochemical Analysis: PCA
Hiroshi Kamitakahara, Tomoki Okayama, Praptiwi, Andria Agusta, Yuki Tobimatsu, Toshiyuki Takano
INTRODUCTION: The rhizome of Angiopteris evecta is of academic interest in Kalimantan, Indonesia, from an ethnobotanical perspective. Angiopteroside is a substance of pharmaceutical importance that is found in the rhizome of A. evecta. OBJECTIVE: The aims of this research are to improve the extraction method for angiopteroside from the rhizome, compared to that in a previous report, and to determine the yield of angiopteroside from the rhizome of A. evecta, as well as to obtain precise data for extractives from the rhizome of A...
September 25, 2018: Phytochemical Analysis: PCA
Ignacio Fernandez-Pastor, Antonio Luque-Muñoz, Francisco Rivas, Marta Medina-O'Donnell, Antonio Martinez, Rafael Gonzalez-Maldonado, Ali Haidour, Andres Parra
INTRODUCTION: L-Dopa, a key neurotransmitter used to treat neural disorders such as Parkinson's disease, is found in the seeds of the genus Mucuna at a sufficient concentration for possible commercial use. OBJECTIVE: To develop a simple and reliable method to extract L-Dopa from M. pruriens seeds in an aqueous medium and then quantitate this compound using a 1 H qNMR method (internal standard); and also to evaluate the accuracy and reproducibility of this method with an NMR calibration curve...
September 14, 2018: Phytochemical Analysis: PCA
Thiago Wolff, Luis A Berrueta, Ligia M M Valente, Rodolfo S Barboza, Rômulo L S Neris, Iris P Guimarães-Andrade, Iranaia Assunção-Miranda, Adriana C Nascimento, Mário Gomes, Blanca Gallo, Carmen Iriondo
INTRODUCTION: The methanol (MeOH) leaf extracts of the species Faramea bahiensis, F. hyacinthina and F. truncata (Rubiaceae) have previously shown in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activities in human hepatocarcinoma cell lineage (HepG2). Chemical studies have led to the isolation of major flavonoids, but quite complex fractions of phenolic compounds still remain. OBJECTIVE: To complete the study of phenolic compounds in the leaves and to access the presence of these compounds in the stems of these Faramea spp...
September 7, 2018: Phytochemical Analysis: PCA
Long Guo, Dan Zhang, Zi-Jing Xue, Qian Jiao, Ai-Peng Liu, Yu-Guang Zheng, E-Hu Liu, Li Duan
INTRODUCTION: Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, two morphologically similar herbal medicines derived from Artemisia genus. Although the two Artemisia herbs have been used as medicines for a long time in China, the study of their phytochemical and bioactive composition is limited. OBJECTIVE: To comprehensively compare and evaluate the composition of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, and find the chemical makers for quality evaluation of the two Artemisia herbal medicines...
September 5, 2018: Phytochemical Analysis: PCA
Leng Wei Khoo, Audrey Siew Foong Kow, Maulidiani Maulidiani, May Yen Ang, Won Yin Chew, Ming Tatt Lee, Chin Ping Tan, Khozirah Shaari, Chau Ling Tham, Faridah Abas
INTRODUCTION: Clinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source. Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage. OBJECTIVE: Evaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach...
September 5, 2018: Phytochemical Analysis: PCA
Camila de Souza Araújo, Ana Paula de Oliveira, Alan Diego da Conceição Santos, Amanda Leite Guimarães, Naiane Darklei Dos Santos Silva, Mário Adriano Ávila Queiroz, Edigênia Cavalcante da Cruz Araújo, Jackson Roberto Guedes da Silva Almeida
INTRODUCTION: Kaurene diterpenes (KDs) constitute a chemical class often found in the genus Annona with interesting biological activities. To date, chromatographic tools have been mostly used to determine KDs. Quantitative nuclear magnetic resonance (qNMR) has distinguished itself in quantitative estimation of natural products and is an interesting choice to assess total KD contents. OBJECTIVE: To establish a 1 H qNMR method for determining the total KD contents in extracts and fractions obtained from Annona vepretorum stems...
August 29, 2018: Phytochemical Analysis: PCA
Yinping Yang, Xinhe Liang, Ping Jin, Na Li, Qiao Zhang, Wei Yan, Hui Zhang, Jiaming Sun
INTRODUCTION: Previous studies have demonstrated that several ginsenosides have remarkable inhibitory effect on acetylcholinesterase (AChE). In the present study, ginseng stem-leaf saponins (GSLS) can improve learning and memory of Alzheimer's disease patients. However, much comprehensive information regarding AChE inhibition of GSLS and its metabolites is yet unknown. OBJECTIVE: The present study aims to screen and determine the potential of AChE inhibitors (AChEIs) from GSLS...
August 29, 2018: Phytochemical Analysis: PCA
Simon Vlad Luca, Anca Miron, Ana Clara Aprotosoaie, Cosmin-Teodor Mihai, Gabriela Vochita, Daniela Gherghel, Nina Ciocarlan, Krystyna Skalicka-Woźniak
INTRODUCTION: Plant species of Verbascum genus have been intensively investigated in the last decades but most studies focused on evaluation of their biological activities; there are only few studies dealing with their chemical characterisation. OBJECTIVE: Detailed investigation of the qualitative and quantitative chemical composition, antioxidant and cytogenotoxic activities of a previously non-studied Verbascum species (V. ovalifolium Donn ex Sims). METHODS: Qualitative analysis of secondary metabolites was performed by HPLC-DAD-ESI-Q-TOF-MS/MS, whereas quantitative data were obtained through HPLC-DAD...
August 28, 2018: Phytochemical Analysis: PCA
Marcelo da Silva Mathias, Rodrigo Rodrigues de Oliveira
INTRODUCTION: Cecropia pachystachya and C. hololeuca are common species in Brazil, popularly used to treat respiratory diseases. Phytochemical studies indicate that their leaves are rich in phenolic compounds, mainly C-glycosilated flavonoids. Orientin and isoorientin are reported in both species, while vitexina and isovitexina were reported in C. pachystachya. In addition, both are rich in chlorogenic acid and have some procyanidins. In some cases, syrups and teas are prepared from leaf blends from different species, which may affect the efficacy and safety of this natural medication...
August 24, 2018: Phytochemical Analysis: PCA
Igor S Flores, Bruno C B Martinelli, Vinicius S Pinto, Luiz H K Queiroz, Luciano M Lião
INTRODUCTION: High-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy enables the analysis of the metabolic profile of plant and animal tissues under close to natural conditions, as well as of other heterogeneous natural or synthetic materials. Neither sample pretreatment is required after fragmentation nor powdering of the sample before insertion into the rotor. However, the efficiency of the method depends strongly on the sample preparation, rotor insertion procedure, and analysis conditions...
August 8, 2018: Phytochemical Analysis: PCA
Yaqian Ma, Menghan Liu, Ting Tan, Aiping Yan, Lan Guo, Kun Jiang, Changheng Tan, Yiqun Wan
INTRODUCTION: Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. OBJECTIVE: To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection...
November 2018: Phytochemical Analysis: PCA
Manon Trinel, Valérie Jullian, Anne-Cécile Le Lamer, Icram Mhamdi, Kember Mejia, Denis Castillo, Billy Joel Cabanillas, Nicolas Fabre
INTRODUCTION: The phytochemistry of the latex of Hura crepitans L. (Euphorbiaceae), a widespread tree in the Amazonian forest having many uses, is little known. Only huratoxin, a daphnane diterpene orthoester, has been described despite the high pharmacological potential of this kind of compounds. Glucosphingolipids (cerebrosides) are also known to be distributed in Euphorbiaceae latexes. OBJECTIVE: To tentatively identify daphnanes diterpenes and cerebrosides in the latex of H...
November 2018: Phytochemical Analysis: PCA
María P Elizalde-González, Erika J Segura-Rivera
INTRODUCTION: Determination of the volatile organic compounds (VOCs) emitted by immature fruits furthers our understanding of plant-pest interactions and by fruits in a ripe state concerns food quality. OBJECTIVES: To apply headspace solid-phase microextraction gas chromatography quadrupole time-of-flight mass spectrometry (HS-SPME-GC-QTOF/MS) to compare the volatiles emitted by different parts of guava (Psidium guajava L. cv. "Media China") at different maturation stages...
November 2018: Phytochemical Analysis: PCA
Steven M Colegate, Roy Upton, Dale R Gardner, Kip E Panter, Joseph M Betz
INTRODUCTION: Pro-toxic dehydropyrrolizidine alkaloids are associated with liver disease in humans. The potential for long-term, low-level or intermittent exposures to cause or contribute to chronically-developing diseases is of international concern. Eupatorium perfoliatum is a medicinal herb referred to as boneset. While the presence of dehydropyrrolizidine alkaloids in some Eupatorium species is well-established, reports on Eupatorium perfoliatum are scant and contradictory. OBJECTIVE: To investigate the presence of dehydropyrrolizidine alkaloids in a survey of boneset samples and related alcoholic tinctures, and hot water infusions and decoctions...
November 2018: Phytochemical Analysis: PCA
Hamidreza Nikpour, Mehdi Mousavi, Hamideh Asadollahzadeh
INTRODUCTION: Teucrium polium is a herbal plant which has curative potential and pharmacological characteristics. Therefore, establishment of accurate analytical methods for characterising its active components is of prime importance. OBJECTIVE: Because of the complex nature of essential oil samples, some results of direct gas chromatography-mass spectrometry (GC-MS) analysis maybe unreliable. Therefore, GC-MS is coupled with chemometric methods to resolve experimental information taken from chromatographic peaks, and assign compounds to them more correctly...
November 2018: Phytochemical Analysis: PCA
Larissa Frankenberger, Tamara D Mora, Carolina D de Siqueira, Fabiola B Filippin-Monteiro, Milene H de Moraes, Maique W Biavatti, Mario Steindel, Louis P Sandjo
INTRODUCTION: The resin of Cola nitida is used in western Cameroon as incense for spiritual protection and during ritual ceremonies. This plant secretion has never been investigated although previous chemical and biological studies on other resins have drawn many attentions. OBJECTIVE: The resin fractions which revealed inhibitory effect on nitric oxide (NO) and tumour necrosis factor alpha (TNF-α) released by lipopolysaccharide (LPS)-activated J774 macrophage as well as on intracellular forms of Leishmania amazonensis and Trypanosoma cruzi amastigote were chemically characterised...
November 2018: Phytochemical Analysis: PCA
Ricardo M Borges, Rahil Taujale, Juliana Santana de Souza, Thaís de Andrade Bezerra, Eder Lana E Silva, Ronny Herzog, Francesca V Ponce, Jean-Luc Wolfender, Arthur S Edison
INTRODUCTION: Dereplication, an approach to sidestep the efforts involved in the isolation of known compounds, is generally accepted as being the first stage of novel discoveries in natural product research. It is based on metabolite profiling analysis of complex natural extracts. OBJECTIVE: To present the application of LipidXplorer for automatic targeted dereplication of phenolics in plant crude extracts based on direct infusion high-resolution tandem mass spectrometry data...
November 2018: Phytochemical Analysis: PCA
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