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Phytochemical Analysis: PCA

Pablo R N Rocha, Flávio A de Freitas, Célio F F Angolini, Lucas-Smith F Vasconcelos, Alaíde L B da Silva, Emmanoel V Costa, Felipe M A da Silva, Marcos N Eberlin, Giovana A Bataglion, Patrícia K Soares, Hector H F Koolen
INTRODUCTION: Alkaloids-derived drugs are a billionaire world-market and several phytochemical investigations aim the identification of new sources and/or structures. Thus, improving the way of extracting and methods to quantify them are of utmost importance. OBJECTIVE: To quantify isoquinoline-derived alkaloids in Unonopsis duckei R.E. Fr. through a new validated high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method in combination with a statistical mixture design that aimed a proper direct extraction of these compounds from leaves...
April 24, 2018: Phytochemical Analysis: PCA
Ming-Zhi Zhu, Gui-Lin Chen, Jian-Lin Wu, Na Li, Zhong-Hua Liu, Ming-Quan Guo
INTRODUCTION: Medicinal plants are gaining increasing attention worldwide due to their empirical therapeutic efficacy and being a huge natural compound pool for new drug discovery and development. The efficacy, safety and quality of medicinal plants are the main concerns, which are highly dependent on the comprehensive analysis of chemical components in the medicinal plants. With the advances in mass spectrometry (MS) techniques, comprehensive analysis and fast identification of complex phytochemical components have become feasible, and may meet the needs, for the analysis of medicinal plants...
April 23, 2018: Phytochemical Analysis: PCA
Yasmin Elsayed, John Refaat, Usama Ramadan Abdelmohsen, Eman Maher Othman, Helga Stopper, Mostafa Ahmed Fouad
INTRODUCTION: Marine sponge-associated actinomycetes are potent sources of bioactive natural products of pharmaceutical significance. They also contributed to the discovery of several clinically relevant antimicrobials. OBJECTIVE: To apply the non-targeted metabolomics approach in chemical profiling of the sponge-derived bacterium Rhodococcus sp. UA13, formerly recovered from the Red Sea sponge Callyspongia aff. Implexa, along with testing for the anti-infective potential of its different fractions...
April 19, 2018: Phytochemical Analysis: PCA
Ashish Sharma, Shilpa Sharma, Mansi Gupta, Shehnaz Fatima, Ravi Saini, Subhash Mohan Agarwal
INTRODUCTION: Natural products exhibit diverse scaffolds and are considered as suitable candidates for development of leads. However, poor pharmacokinetics often acts as a hindrance during the drug discovery process. OBJECTIVE: With a view of exploring the absorption, distribution, metabolism, excretion and toxicity (ADMET) profile of plant-based anticancer compounds, open-access databases (NPACT, CancerHSP and TaxKB) were analysed to identify molecules having properties favourable for drug ability...
April 18, 2018: Phytochemical Analysis: PCA
Liang Qin, Yawen Zhang, Yaqin Liu, Huixin He, Manman Han, Yanyan Li, Maomao Zeng, Xiaodong Wang
INTRODUCTION: Mass spectrometry imaging (MSI) as a label-free and powerful imaging technique enables in situ evaluation of a tissue metabolome and/or proteome, becoming increasingly popular in the detection of plant endogenous molecules. OBJECTIVE: The characterization of structure and spatial information of endogenous molecules in plants are both very important aspects to better understand the physiological mechanism of plant organism. METHODS: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a commonly-used tissue imaging technique, which requires matrix to assist in situ detection of a variety of molecules on the surface of a tissue section...
April 17, 2018: Phytochemical Analysis: PCA
Miao-Miao Jin, Wen-Dan Zhang, Hong-Hong Jiang, Ying-Feng Du, Wei Guo, Liang Cao, Hui-Jun Xu
INTRODUCTION: Triterpenoid saponins are the major bioactive constituents of Pulsatilla chinensis, playing an important role in various biological activities such as anti-tumour, cognition-enhancing, anti-biosis, anti-inflammatory, hypoglycemic and immunological adjuvant. OBJECTIVE: To establish a systematic strategy based on ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) for the efficient characterisation and identification of triterpenoid saponins in crude extracts from Pulsatilla chinensis...
April 10, 2018: Phytochemical Analysis: PCA
Ying Liang, Guan-Yu Yan, Jian-Lin Wu, Xingxing Zong, Zhongqiu Liu, Hua Zhou, Liang Liu, Na Li
INTRODUCTION: Lipo-alkaloid (LA) is a kind of C19 -norditerpenoid alkaloid in Aconitum species, which usually contains an aconitane skeleton and one or two fatty acid residues. OBJECTIVE: To qualify and quantify the fatty acids and lipo-alkaloids in Aconitum carmichaelii. METHODOLOGY: An ultra-high performance liquid chromatography-triple quadrupole-mass spectrometry (UHPLC-QQQ-MS) method was established to quantify LAs, while the free fatty acids were identified by gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UHPLC-Q-TOF-MS)...
March 30, 2018: Phytochemical Analysis: PCA
Matthew Noestheden, Gareth Friedlander, Jason Anspach, Scott Krepich, K C Hyland, Wesley F Zandberg
INTRODUCTION: Cannabis sativa L. (cannabis) is utilised as a therapeutic and recreational drug. With the legalisation of cannabis in many countries and the anticipated regulation of potency that will accompany legalisation, analytical testing facilities will require a broadly applicable, quantitative, high throughput method to meet increased demand. Current analytical methods for the biologically active components of cannabis (phytocannabinoids) suffer from low throughput and/or an incomplete complement of relevant phytocannabinoids...
March 30, 2018: Phytochemical Analysis: PCA
Shengli Han, Yanni Lv, Fen Wei, Jia Fu, Qi Hu, Sicen Wang
INTRODUCTION: Cell membrane chromatography (CMC), as a highly selective type of affinity chromatography, has been demonstrated as an effective method to screen bioactive components acting on specific receptor from a complicated biological system. OBJECTIVE: To review the recent research progress and the technical applications of these analytical methods using CMC combined with gas chromatography-mass spectrometry, (GC/MS) and liquid chromatography-mass spectrometry (LC/MS)...
March 24, 2018: Phytochemical Analysis: PCA
Jinghui Sun, Chuanxin Jiao, Yue Ma, Jianguang Chen, Wei Wu, Shuying Liu
INTRODUCTION: Red ginseng (RG) is one of the main processed products of the roots and rhizomes of Panax ginseng C.A. Meyer and is used for anti-ageing. But how metabonomic influences of RG on the progress of ageing are less researched. OBJECTIVE: A metabonomic method was developed to study the characters of the ageing process and the effects of total ginsenosides of red ginseng (TGRG) on the progress of ageing. METHODS: Urine samples from four different ages (4, 12, 18 and 24 months old) of rats and interference after TGRG were analysed by rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) and multivariate statistical analysis were performed for the pattern recognition and characteristic metabolites identification...
March 24, 2018: Phytochemical Analysis: PCA
Hanqing Wang, Dong Xiao, Wei Li, Xia Zhang, Xiaojuan Gao, Jingjiao Yong, Jianjun Zhao, Kazuo Koike
INTRODUCTION: Different parts of Sophora alopecuroides L. (Fabaceae) have historically been used in traditional Chinese medicine for the treatment of dysentery and enteritis. This plant is also utilised as an important resource for industrial preparation of quinolizidine alkaloidal pharmaceuticals. OBJECTIVE: Establish a reliable, simple and fast analytical method for the quantitative determination of the quinolizidine-type alkaloids and extend understanding of the metabolism of quinolizidine-type alkaloids in S...
March 24, 2018: Phytochemical Analysis: PCA
Sung Hum Yeon, Je-Seung Jeon, Key An Um, Chul Young Kim, Young-Joon Ahn
INTRODUCTION: Secologanic acid, a major secoiridoid in the flower buds of Lonicera japonica, is a fragile, highly polar compound that readily changes to epivogeloside or vogeloside after being dissolved in methanol. Thus, it is very difficult to obtain secologanic acid on a large-scale. OBJECTIVE: To develop a centrifugal partition chromatography (CPC) method for large-scale purification of secologanic acid with high purity from the flower buds of L. japonica. METHODS: After fractionation with Diaion HP-20 macroporous resin, 30% methanol eluent was purified by CPC with a ternary biphasic solvent system with ethyl acetate/isopropanol/water (6:4:10, v/v/v)...
March 9, 2018: Phytochemical Analysis: PCA
Riitta Tegelberg, Virpi Virjamo, Riitta Julkunen-Tiitto
INTRODUCTION: In ecological experiments, storage of plant material is often needed between harvesting and laboratory analyses when the number of samples is too large for immediate, fresh analyses. Thus, accuracy and comparability of the results call for pre-treatment methods where the chemical composition remains unaltered and large number of samples can be treated efficiently. OBJECTIVE: To study if a fast dry-air drying provides an efficient pre-treatment method for quantitative analyses of phenolics...
March 9, 2018: Phytochemical Analysis: PCA
Erica Cusano, Roberto Consonni, Eleftherios A Petrakis, Konstantina Astraka, Laura R Cagliani, Moschos G Polissiou
INTRODUCTION: The increasing interest on Crocus sativus L. over the last decades is caused by its potential employment as a source of biologically active molecules, endowed with antioxidant and nutraceutical properties. These molecules are present mainly in stigmas and tepals, these last generally considered as byproducts. OBJECTIVE: To characterise bioactive compounds in stigmas, stamens, and tepals of Crocus sativus L. for quality, cross-contamination of tissues or fraudulent addition, joining spectroscopic and chromatographic techniques...
February 26, 2018: Phytochemical Analysis: PCA
Anaí L Dos Santos, Antônio da Silva Novaes, Allan Dos S Polidoro, Márcio Eduardo de Barros, Jonas S Mota, Daiane B M Lima, Laiza C Krause, Cláudia A L Cardoso, Rosângela A Jacques, Elina B Caramão
INTRODUCTION: Piper amalago has a distribution from Mexico to Brazil; their aerial parts have been used in folk medicine to treat diuretic and kidney diseases. OBJECTIVE: The purpose of this study was to obtain a deeper understanding of the chemical composition of essential oils (EOs) extracted from both the leaves and stems of P. amalago, compare them, and evaluate their antilithiasic activity and acute toxicity. METHODOLOGY: Extraction was performed by hydrodistillation, whereas chemical characterisation by two-dimensional gas chromatography coupled with rapid-scanning quadrupole mass spectrometry (GC×GC/qMS)...
February 26, 2018: Phytochemical Analysis: PCA
Bastien Durenne, Alodie Blondel, Philippe Druart, Marie-Laure Fauconnier
INTRODUCTION: The dynamic headspace sampling technique using thermal desorption, gas chromatography-mass spectrometry (TD-GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs), and experiments performed in sterile and controlled conditions can be useful for VOC metabolism investigations. OBJECTIVE: The main purpose of this study was to set up a laboratory high-throughput glass chamber for whole plant volatiles analysis. Brassica napus L...
February 20, 2018: Phytochemical Analysis: PCA
Wei Wu, Chuanxi Jiao, Hui Li, Yue Ma, Lili Jiao, Shuying Liu
INTRODUCTION: Panax ginseng has received much attention as a valuable health supplement with medicinal potential. Its chemical diversity and multiple pharmacological properties call for comprehensive methods to better understand the effects of ginseng and ginsenosides. Liquid chromatography-mass spectrometry (LC-MS) based metabonomic approaches just fit the purpose. OBJECTIVE: Aims to give a review of recent progress on LC-MS based pharmacokinetic, metabolic, and phytochemical metabolomic studies of ginseng, and metabonomic studies of ginseng intervention effects...
February 19, 2018: Phytochemical Analysis: PCA
Woo Sik Ham, Jinju Kim, Dae Joon Park, Han-Cheol Ryu, Young Pyo Jang
INTRODUCTION: Precise identification of botanical origin of plant species is crucial for the quality control of herbal medicine. In Korea, the root part of Cynanchum auriculatum has been misused for C. wilfordii in the herbal drug market due to their morphological similarities. Currently, DNA analysis using the polymerase chain reaction (PCR) method is employed to discriminate between these species. OBJECTIVE: In order to develop a new analytical tool for the rapid discrimination of C...
February 12, 2018: Phytochemical Analysis: PCA
Eman Shawky, Rasha M Abou El Kheir
INTRODUCTION: Species of Apiaceae are used in folk medicine as spices and in officinal medicinal preparations of drugs. They are an excellent source of phenolics exhibiting antioxidant activity, which are of great benefit to human health. Discrimination among Apiaceae medicinal herbs remains an intricate challenge due to their morphological similarity. OBJECTIVE: In this study, a combined "untargeted" and "targeted" approach to investigate different Apiaceae plants species was proposed by using the merging of high-performance thin layer chromatography (HPTLC)-image analysis and pattern recognition methods which were used for fingerprinting and classification of 42 different Apiaceae samples collected from Egypt...
February 11, 2018: Phytochemical Analysis: PCA
Lydia Siegle, Jörg Pietsch
INTRODUCTION: Taxus baccata L. is an evergreen conifer whose plant parts are cardiotoxic. Only the red arils of the berries are described as non-toxic and taxane-free. OBJECTIVE: Extraction and HPLC-MS/MS methods were developed for the investigation of the Taxus compounds 3,5-dimethoxyphenol, 10-deacetylbaccatin III, baccatin III, cephalomannine, taxol A and taxinine M in the red arils of the yew berries. MethodologyA liquid-liquid extraction method for the red arils of the fruits from three yews were developed...
February 9, 2018: Phytochemical Analysis: PCA
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