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Phytochemical Analysis: PCA

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https://www.readbyqxmd.com/read/27910224/separating-and-identifying-the-four-stereoisomers-of-methyl-jasmonate-by-rp-hplc-and-using-cyclodextrins-in-a-novel-way
#1
Adrián Matencio, Mario J Bermejo-Gimeno, Francisco García-Carmona, José Manuel López-Nicolás
INTRODUCTION: Several authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. OBJECTIVE: (1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910216/capillary-electrophoresis-as-tool-for-diastereomeric-separation-in-a-trichilia-catigua-fraction
#2
Ana Luiza Sereia, Renata Longhini, Gisely Cristiny Lopes, João Carlos Palazzo de Mello
INTRODUCTION: The tree Trichilia catigua, popularly known as "catuaba", shows several biological activities and has emerged as a potential source of new drugs. Considering that more than 10 species are known under the same popular name, regulatory agencies require more rigorous quality control of this medicinal plant. OBJECTIVE: To develop and validate a methodology using capillary electrophoresis (CE) with ultraviolet (UV) detection for analysing polyphenols in the ethyl-acetate fraction (EAF) of Trichilia catigua...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910199/reverse-phase-compatible-tlc-bioautography-for-detection-of-tyrosinase-inhibitors
#3
Paula García, I Ayelen Ramallo, Ricardo L E Furlan
INTRODUCTION: Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. OBJECTIVES: To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. METHODS: Enzyme gel entrapment with an amphiphilic copolymer was used for assay development...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910158/hptlc-bioautography-guided-isolation-of-%C3%AE-glucosidase-inhibiting-compounds-from-justicia-secunda-vahl-acanthaceae
#4
Barbara A Theiler, Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O Espinoza Caisa, Sabine Glasl
INTRODUCTION: α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from the leaves of Justicia secunda Vahl are used to treat diabetes mellitus symptoms. OBJECTIVE: To screen for α-glucosidase inhibitors in J. secunda via HPTLC bioautography...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910174/combined-application-of-uhplc-qtof-ms-hplc-elsd-and-1-h-nmr-spectroscopy-for-quality-assessment-of-da-9801-a-standardised-dioscorea-extract
#5
Kyo Bin Kang, Jayoung Ryu, Youngwoong Cho, Sang-Zin Choi, Miwon Son, Sang Hyun Sung
INTRODUCTION: DA-9801, a standardised 50% aqueous ethanolic extract of a mixture of Dioscorea japonica and D. nipponica, is a botanical drug candidate for the treatment of diabetic neuropathy, which finished its US phase II clinical trials recently. An advanced quality control method is needed for further development of DA-9801, considering its high contents of both primary and secondary metabolites. OBJECTIVE: Development of a quality assessment strategy for DA-9801, based on the combination of UHPLC-QTOF/MS, HPLC-ELSD, and (1) H-NMR spectroscopy...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910164/hplc-uv-and-lc-ms-analyses-of-acylquinic-acids-in-geigeria-alata-dc-oliv-hiern-and-their-contribution-to-antioxidant-and-antimicrobial-capacity
#6
Dimitrina Zheleva-Dimitrova, Reneta Gevrenova, Maya M Zaharieva, Hristo Najdenski, Silviya Ruseva, Valentin Lozanov, Vessela Balabanova, Sakina Yagi, Georgi Momekov, Vanio Mitev
INTRODUCTION: Geigeria alata is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints. OBJECTIVE: To analyze phenolic acids in Geigeria alata roots and leaves and to evaluate their antioxidant and antimicrobial activities. METHODOLOGY: Phenolic acids in the aqueous-methanol extracts were identified by LC-MS. Major compounds were isolated using low-pressure liquid chromatography...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27880856/direct-analysis-in-real-time-mass-spectrometry-for-rapid-identification-of-traditional-chinese-medicines-with-coumarins-as-primary-characteristics
#7
Zhiyong Chen, Yuanyuan Yang, Hongxun Tao, Liping Liao, Ye Li, Zijia Zhang
INTRODUCTION: The increasing popularity of traditional Chinese medicines (TCMs) necessitates rapid and reliable methods for controlling their quality. Direct analysis in real-time mass spectrometry (DART-MS) represents a novel approach to analysing TCMs. OBJECTIVE: To develop a quick and reliable method of identifying TCMs with coumarins as primary characteristics. METHODOLOGY: DART-MS coupled with ion trap mass spectrometry was employed to rapidly identify TCMs with coumarins as primary characteristics and to explore the ionisation mechanisms of simple coumarins, furocoumarins and pyranocoumarins in detail...
November 23, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27688003/high-triterpenic-acids-production-in-callus-cultures-from-fruit-pulp-of-two-apple-varieties
#8
Giancarlo Verardo, Andrea Gorassini, Donata Ricci, Daniele Fraternale
INTRODUCTION: Very rarely fruit pulp has been used in in vitro culture to produce secondary metabolites useful in promoting health. OBJECTIVES: The aims of this work were the study of the best conditions to obtain the callus cultures from the pulp of two varieties of apples, Golden Delicious (GD) and "Mela Rosa Marchigiana" (MRM), and the quali-quantitative analysis of secondary metabolites produced by the two in vitro callus cultures. METHODOLOGY: Callus was induced on both Murashige and Skoog and Gamborg B5 media containing various combinations of supplements...
September 30, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27690371/economical-plain-and-rapid-authentication-of-actaea-racemosa-l-syn-cimicifuga-racemosa-black-cohosh-herbal-raw-material-by-resilient-rp-pda-hplc-and-chemometric-analysis
#9
Marian Bittner, Regina Schenk, Andreas Springer, Matthias F Melzig
INTRODUCTION: The medicinal plant Actaea racemosa L. (Ranunculaceae, aka black cohosh) is widely used to treat climacteric complaints as an alternative to hormone substitution. Recent trials prove efficacy and safety of the approved herbal medicinal products from extracts of pharmaceutical quality. This led to worldwide increasing sales. A higher demand for the plant material results in problems with economically motivated adulteration. Thus, reliable tools for herbal drug authentication are necessary...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27689319/field-based-evaluation-of-a-novel-spme-gc-ms-method-for-investigation-of-below-ground-interaction-between-brassica-roots-and-larvae-of-cabbage-root-fly-delia-radicum-l
#10
William Deasy, Tom Shepherd, Colin J Alexander, A Nicholas E Birch, K Andrew Evans
INTRODUCTION: Collection of volatiles from plant roots poses technical challenges due to difficulties accessing the soil environment without damaging the roots. OBJECTIVES: To validate a new non-invasive method for passive sampling of root volatiles in situ, from plants grown under field conditions, using solid phase micro-extraction (SPME). METHODS: SPME fibres were inserted into perforated polytetrafluoroethene (PTFE) tubes positioned in the soil next to broccoli plants for collection of root volatiles pre- and post-infestation with Delia radicum larvae...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27687886/development-and-validation-of-a-spme-gc-ms-method-for-in-situ-passive-sampling-of-root-volatiles-from-glasshouse-grown-broccoli-plants-undergoing-below-ground-herbivory-by-larvae-of-cabbage-root-fly-delia-radicum-l
#11
William Deasy, Tom Shepherd, Colin J Alexander, A Nicholas E Birch, K Andrew Evans
INTRODUCTION: Research on plant root chemical ecology has benefited greatly from recent developments in analytical chemistry. Numerous reports document techniques for sampling root volatiles, although only a limited number describe in situ collection. OBJECTIVES: To demonstrate a new method for non-invasive in situ passive sampling using solid phase micro extraction (SPME), from the immediate vicinity of growing roots. METHODS: SPME fibres inserted into polyfluorotetrafluoroethylene (PTFE) sampling tubes located in situ which were either perforated, covered with stainless steel mesh or with microporous PTFE tubing, were used for non-invasive sub-surface sampling of root volatiles from glasshouse-grown broccoli...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27687791/needle-trap-device-as-a-new-sampling-and-preconcentration-approach-for-volatile-organic-compounds-of-herbal-medicines-and-its-application-to-the-analysis-of-volatile-components-in-viola-tianschanica
#12
Yan Qin, Yingming Pang, Zhihong Cheng
INTRODUCTION: The needle trap device (NTD) technique is a new microextraction method for sampling and preconcentration of volatile organic compounds (VOCs). Previous NTD studies predominantly focused on analysis of environmental volatile compounds in the gaseous and liquid phases. Little work has been done on its potential application in biological samples and no work has been reported on analysis of bioactive compounds in essential oils from herbal medicines. OBJECTIVE: The main purpose of the present study is to develop a NTD sampling method for profiling VOCs in biological samples using herbal medicines as a case study...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27687704/in-situ-dart-ms-as-a-versatile-and-rapid-dereplication-tool-in-lichenology-chemical-fingerprinting-of-ophioparma-ventosa
#13
Pierre Le Pogam, Anne-Cécile Le Lamer, Béatrice Legouin, Joël Boustie, David Rondeau
INTRODUCTION: Lichens widely occur all over the world and are known to produce unique secondary metabolites with various biological activities. OBJECTIVE: To develop high-throughput screening approaches requiring little to no sample preparation to alleviate the dereplication holdup and accelerate the discovery workflow of new structures from lichens. METHODOLOGY: The extracellular distribution of lichen metabolites is incentive for in situ chemical profiling of lichens using the ambient mass spectrometry DART-MS...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27687607/the-identification-and-quantification-of-suberin-monomers-of-root-and-tuber-periderm-from-potato-solanum-tuberosum-as-fatty-acyl-tert-butyldimethylsilyl-derivatives
#14
Dolors Company-Arumí, Mercè Figueras, Victoria Salvadó, Marisa Molinas, Olga Serra, Enriqueta Anticó
INTRODUCTION: Protective plant lipophilic barriers such as suberin and cutin, with their associated waxes, are complex fatty acyl derived polyesters. Their precise chemical composition is valuable to understand the specific role of each compound to the physiological function of the barrier. OBJECTIVES: To develop a method for the compositional analysis of suberin and associated waxes by gas chromatography (GC) coupled to ion trap-mass spectrometry (IT-MS) using N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) as sylilating reagent, and apply it to compare the suberin of the root and tuber periderm of potato (Solanum tuberosum)...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27438090/an-enzyme-linked-immunosorbent-assay-for-genistein-7-o-%C3%AE-rhamnopyranosyl-1%C3%A2-6-%C3%AE-glucopyranoside-determination-in-derris-scandens-using-a-polyclonal-antibody
#15
Kamonthip Jutathis, Tharita Kitisripanya, Orain Udomsin, Chadathorn Inyai, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun
INTRODUCTION: Genistein 7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside (GTG) is a major bioactive compound in Derris scandens. It is responsible for anti-inflammatory activity by inhibition of cyclooxygenase and lipoxygenase. There are many commercial products of D. scandens available in Thailand. OBJECTIVE: To develop an enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of GTG in plant material and derived products using a polyclonal antibody...
November 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27527356/utilization-of-the-ability-to-induce-activation-of-the-nuclear-factor-erythroid-derived-2-like-factor-2-nrf2-to-assess-potential-cancer-chemopreventive-activity-of-liquorice-samples
#16
Norazah Basar, Lutfun Nahar, Olayinka Ayotunde Oridupa, Kenneth J Ritchie, Anupam D Talukdar, Angela Stafford, Habibjon Kushiev, Asuman Kan, Satyajit D Sarker
INTRODUCTION: Nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) is a transcription factor that regulates expression of many detoxification enzymes. Nrf2-antioxidant responsive element (Nrf2-ARE) signalling pathway can be a target for cancer chemoprevention. Glycyrrhiza glabra, common name, 'liquorice', is used as a sweetening and flavouring agent, and traditionally, to treat various ailments, and implicated to chemoprevention. However, its chemopreventive property has not yet been scientifically substantiated...
September 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27439115/supercritical-extraction-of-scopoletin-from-helichrysum-italicum-roth-g-don-flowers
#17
Stela Jokić, Marina Rajić, Blanka Bilić, Maja Molnar
INTRODUCTION: The increasing popularity of immortelle (Helichrysum italicum (Roth) G. Don) and its products, particularly in the cosmetic industry, is evident nowadays. This plant is a source of coumarins, especially scopoletin, which are highly soluble in supercritical CO2 . OBJECTIVE: The objective of this study was to perform the supercritical CO2 extraction process of Helichrysum italicum flowers at different values of pressure and temperature and to optimise the extraction process using response surface methodology in terms of obtaining the highest extraction yield and yield of extracted scopoletin...
September 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27439018/gc-ms-and-q-nmr-based-chemotaxonomic-evaluation-of-two-leonurus-species
#18
Nilufar Z Mamadalieva, Khayrulla M Bobakulov, Vittorio Vinciguerra, Antonio Tiezzi, Nasrulla D Abdullaev, Lutfun Nahar, Shahnoz S Azimova, Satyajit D Sarker
INTRODUCTION: The genus Leonurus L. (fam: Lamiaceae) is represented in Uzbekistan by two species, L. panzerioides Popov. and L. turkestanicus V. I. Krecz. & Kuprian, which are used to treat nervous disorders and also as sedative and hypotensive agents. OBJECTIVES: To establish the taxonomic status of Leonurus panzerioides and L. turkestanicus based on their chemical constituents analysed by GC-MS and q-NMR. MATERIALS AND METHODS: Quantitative (1) H-NMR (q-NMR) was used to identify and quantify known major components in the methanol extracts of these two species...
September 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27438906/ultra-high-performance-liquid-chromatography-with-photodiode-array-and-chemiluminescence-detection-for-the-determination-of-polyphenolic-antioxidants-in-erigeron-acris-l-extracts
#19
Julita Malejko, Edyta Nalewajko-Sieliwoniuk, Julia Szabuńko, Jolanta Nazaruk
INTRODUCTION: The quality of herbs is directly related to the presence of polyphenolic antioxidants. This is the first report on the quantification of individual polyphenolic constituents of Erigeron acris L. OBJECTIVE: To develop a new method using ultra-high performance liquid chromatography with photodiode array and chemiluminescence (UHPLC-PDA-CL) detection for the separation and determination of polyphenols in Erigeron acris extracts. METHODOLOGY: The methanolic extracts from leaves and inflorescences of Erigeron acris were prepared by ultrasound assisted extraction...
September 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27438776/profiling-and-simultaneous-quantitative-determination-of-anthocyanins-in-wild-myrtus-communis-l-berries-from-different-geographical-areas-in-sardinia-and-their-comparative-evaluation
#20
Mariateresa Maldini, Mario Chessa, Giacomo L Petretto, Paola Montoro, Jonathan P Rourke, Marzia Foddai, Marcello Nicoletti, Giorgio Pintore
INTRODUCTION: Myrtus communis L. (Myrtaceae) is a self-seeded shrub, widespread in Sardinia, with anti-inflammatory, antiseptic, antimicrobial, hypoglycemic and balsamic properties. Its berries, employed for the production of sweet myrtle liqueur, are characterised by a high content of bioactive polyphenols, mainly anthocyanins. Anthocyanin composition is quite specific for vegetables/fruits and can be used as a fingerprint to determine the authenticity, geographical origin and quality of raw materials, products and extracts...
September 2016: Phytochemical Analysis: PCA
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