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Phytochemical Analysis: PCA

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https://www.readbyqxmd.com/read/28092423/-1-h-qnmr-quantification-of-annonaceous-acetogenins-in-crude-extracts-of-annona-muricata-l-fruit-pulp
#1
Natacha Bonneau, Timothé Cynober, Jean-Christophe Jullian, Pierre Champy
INTRODUCTION: Annonaceous acetogenins (AAGs) constitute a group of environmental neurotoxins, possibly implicated in sporadic atypical Parkinsonism/dementia complexes. The recent evidencing of complex mixtures of AAGs in edible fruits and derived food products requires efficient and practical analytical tools for an estimation of human exposure. OBJECTIVE: To develop a simple method for the direct quantitation of the majority of AAGs (sub-types 1a and 1b) within crude extracts, using commonly available (1) H-NMR spectrometers, for food control...
January 16, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28071864/simultaneous-determination-of-four-triterpenoid-saponins-in-aralia-elata-leaves-by-hplc-elsd-combined-with-hierarchical-clustering-analysis
#2
Yichun Sun, Baimei Li, Xiaoting Lin, Juan Xue, Zhibin Wang, Hongwei Zhang, Hai Jiang, Qiuhong Wang, Haixue Kuang
INTRODUCTION: Aralia elata leaves are known to have several biological activities, including anti-arrythmia, antitumor, anti-inflammatory, anti-fatigue, antimicrobial and antiviral effects. Our previous study found that triterpenoid saponins from the leaves of A. elata had antitumor effects. Quantification of the triterpenoids is important for the quality control of A. elata leaves. OBJECTIVE: To establish high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) for the simultaneous determination of four major triterpenoid saponins, including Aralia-saponin IV, Aralia-saponin VI, 3-O-β-d- glucopyranosyl-(1 → 3)-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside (Aralia-saponin TTP)and Aralia-saponin V...
January 10, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28067965/rp-hplc-dad-esi-qtof-ms-based-metabolic-profiling-of-the-potential-olea-europaea-by-product-wood-and-its-comparison-with-leaf-counterpart
#3
Sonda Ammar, Maria Del Mar Contreras, Boutheina Gargouri, Antonio Segura-Carretero, Mohamed Bouaziz
INTRODUCTION: Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition. OBJECTIVE: That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses...
January 9, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28029194/reverse-iontophoretic-extraction-of-metabolites-from-living-plants-and-their-identification-by-ion-chromatography-coupled-to-high-resolution-mass-spectrometry
#4
Maria Isabel González Sánchez, James McCullagh, Richard H Guy, Richard G Compton
INTRODUCTION: The identification and characterisation of cellular metabolites has now become an important strategy to obtain insight into functional plant biology. However, the extraction of metabolites for identification and analysis is challenging and, at the present time, usually requires destruction of the plant. OBJECTIVE: To detect different plant metabolites in living plants with no pre-treatment using the combination of iontophoresis and ion-chromatography with mass spectrometry detection...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28028887/phytochemical-analysis-of-a-cytotoxic-fraction-of-quassia-silvestris-using-lc-hr-esi-ms-n
#5
Michel Feussi Tala, Ferdinand Mouafo Talontsi, Guang-Zhi Zeng, Hippolyte Kamdem Wabo, Michael Spiteller, Ning-Hua Tan, Pierre Tane
INTRODUCTION: The genus Quassia is a promising source of secondary metabolites with biological potential including antimalarial and cytotoxic activities. Limited data are available on the phytochemistry and pharmacology of Quassia silvestris Cheek & Jongkind, a Cameroonian medicinal plant used to treat various ailments. OBJECTIVES: To carry out the bioassay-guided fractionation and LC-HR-ESI-MS analyses of the leaves extract from Q. silvestris; to purify the active fractions and isolate the major compounds using different chromatographic and spectroscopic methods...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28028854/hplc-elsd-quantification-and-centrifugal-partition-chromatography-isolation-of-8-o-acetylharpagide-from-oxera-coronata-lamiaceae
#6
Camille Remeur, Erell Le Borgne, Léa Gauthier, Raphaël Grougnet, Brigitte Deguin, Cyril Poullain, Marc Litaudon
INTRODUCTION: Iridoid glycosides possess highly functionalised monoterpenoid aglycon with several contiguous stereocentres. For the most common, they are often present in quantities reaching several percentage of the fresh plant weight, and thus they may be regarded as starting material for the synthesis of a number of new chiral and bioactive molecules. OBJECTIVE: To quantify and to isolate 8-O-acetylharpagide (AH) from several extracts of Oxera coronata R.P.J...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28028844/improved-tlc-bioautographic-assay-for-qualitative-and-quantitative-estimation-of-tyrosinase-inhibitors-in-natural-products
#7
Jinge Zhou, Qingjiu Tang, Tao Wu, Zhihong Cheng
INTRODUCTION: TLC bioautography for tyrosinase inhibitors has made recent progress; however, an assay with a relative low consumption of enzyme and quantitative capability would greatly advance the efficacy of related TLC bioautographic assays. OBJECTIVE: An improved TLC bioautographic assay for detecting tyrosinase inhibitors was developed and validated in this study. METHODS: L-DOPA (better water-solubility than L-tyrosine) was used as the substrate instead of reported L-tyrosine...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28008675/exploring-%C3%AE-tubulin-inhibitors-from-plant-origin-using-computational-approach
#8
Kanika Verma, Kaavya Kannan, Shanthi V, Sethumadhavan R, Karthick V, Ramanathan K
INTRODUCTION: β-Tubulin is an important target for the binding of anti-cancer drugs, in particular, paclitaxel (taxol), vinblastine and epothilone. However, mutations in β-tubulin structure give resistance to chemotherapeutic agents. Notably, mutations at R306C, F270 V, L217R, L228F, A185T and A248V positions in β-tubulin give high resistance for paclitaxel binding. OBJECTIVE: To discover novel inhibitors of β-tubulin from natural sources, particularly alkaloids, using a virtual screening approach...
December 23, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28008674/understanding-the-effects-of-roasting-on-antioxidant-components-of-coffee-brews-by-coupling-on-line-abts-assay-to-high-performance-size-exclusion-chromatography
#9
Sebastian E W Opitz, Bernard A Goodman, Marco Keller, Samo Smrke, Marco Wellinger, Stefan Schenker, Chahan Yeretzian
INTRODUCTION: Coffee is a widely consumed beverage containing antioxidant active compounds. During roasting the phytochemical composition of the coffee bean changes dramatically and highly polymeric substances are produced. Besides chlorogenic acids that are already present in green coffee beans, melanoidins show antioxidant capacity as well. OBJECTIVE: To employ post-column derivatisation by coupling high performance size exclusion chromatography (HPSEC) to an antioxidant assay to investigate the effect of roasting on the properties of antioxidant active compounds in coffee brews...
December 23, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27995663/phytoelectrochemical-analysis-of-zanthoxylum-chiloperone
#10
Gerardo Cebrián-Torrejón, Antonio Doménech-Carbó, Bruno Figadère, Erwan Poupon, Alain Fournet
INTRODUCTION: An innovative application of the voltammetry of microparticles methodology to characterize the phytochemical composition of extracts of different parts of Zanthoxylum chiloperone var. angustifolium Engl. is described. OBJECTIVE: Characterize the phytochemical composition of extracts of different parts of plants by electrochemical methodologies. METHODS: The voltammetry of microparticles methodology was applied to alcoholic extracts from leaves, seeds, fruits, roots and stem bark of Zanthoxylum chiloperone...
December 19, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27981663/determination-of-polyphenolic-phytochemicals-using-highly-deshielded-oh-1-h-nmr-signals
#11
Pantelis Charisiadis, Vassiliki G Kontogianni, Constantinos G Tsiafoulis, Andreas G Tzakos, Ioannis P Gerothanassis
No abstract text is available yet for this article.
December 15, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27921344/uhplc-ms-based-hdac-assay-applied-to-bio-guided-microfractionation-of-fungal-extracts
#12
Vincent Zwick, Pierre-Marie Allard, Lucie Ory, Claudia A Simões-Pires, Laurence Marcourt, Katia Gindro, Jean-Luc Wolfender, Muriel Cuendet
INTRODUCTION: Histone deacetylases (HDAC) are considered as promising targets for cancer treatment. Today, four HDAC inhibitors, vorinostat, romidepsin, belinostat, and panobinostat, have been approved by the Food and Drug Administration (FDA) for cancer treatment, while others are in clinical trials. Among them, several are naturally occurring fungal metabolites. OBJECTIVE: To develop and optimise an enzyme assay for bio-guided identification of HDAC inhibitors in fungal strains...
December 5, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910224/separating-and-identifying-the-four-stereoisomers-of-methyl-jasmonate-by-rp-hplc-and-using-cyclodextrins-in-a-novel-way
#13
Adrián Matencio, Mario J Bermejo-Gimeno, Francisco García-Carmona, José Manuel López-Nicolás
INTRODUCTION: Several authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. OBJECTIVE: (1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910216/capillary-electrophoresis-as-tool-for-diastereomeric-separation-in-a-trichilia-catigua-fraction
#14
Ana Luiza Sereia, Renata Longhini, Gisely Cristiny Lopes, João Carlos Palazzo de Mello
INTRODUCTION: The tree Trichilia catigua, popularly known as "catuaba", shows several biological activities and has emerged as a potential source of new drugs. Considering that more than 10 species are known under the same popular name, regulatory agencies require more rigorous quality control of this medicinal plant. OBJECTIVE: To develop and validate a methodology using capillary electrophoresis (CE) with ultraviolet (UV) detection for analysing polyphenols in the ethyl-acetate fraction (EAF) of Trichilia catigua...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910199/reverse-phase-compatible-tlc-bioautography-for-detection-of-tyrosinase-inhibitors
#15
Paula García, I Ayelen Ramallo, Ricardo L E Furlan
INTRODUCTION: Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. OBJECTIVES: To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. METHODS: Enzyme gel entrapment with an amphiphilic copolymer was used for assay development...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910158/hptlc-bioautography-guided-isolation-of-%C3%AE-glucosidase-inhibiting-compounds-from-justicia-secunda-vahl-acanthaceae
#16
Barbara A Theiler, Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O Espinoza Caisa, Sabine Glasl
INTRODUCTION: α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from the leaves of Justicia secunda Vahl are used to treat diabetes mellitus symptoms. OBJECTIVE: To screen for α-glucosidase inhibitors in J. secunda via HPTLC bioautography...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910174/combined-application-of-uhplc-qtof-ms-hplc-elsd-and-1-h-nmr-spectroscopy-for-quality-assessment-of-da-9801-a-standardised-dioscorea-extract
#17
Kyo Bin Kang, Jayoung Ryu, Youngwoong Cho, Sang-Zin Choi, Miwon Son, Sang Hyun Sung
INTRODUCTION: DA-9801, a standardised 50% aqueous ethanolic extract of a mixture of Dioscorea japonica and D. nipponica, is a botanical drug candidate for the treatment of diabetic neuropathy, which finished its US phase II clinical trials recently. An advanced quality control method is needed for further development of DA-9801, considering its high contents of both primary and secondary metabolites. OBJECTIVE: Development of a quality assessment strategy for DA-9801, based on the combination of UHPLC-QTOF/MS, HPLC-ELSD, and (1) H-NMR spectroscopy...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910164/hplc-uv-and-lc-ms-analyses-of-acylquinic-acids-in-geigeria-alata-dc-oliv-hiern-and-their-contribution-to-antioxidant-and-antimicrobial-capacity
#18
Dimitrina Zheleva-Dimitrova, Reneta Gevrenova, Maya M Zaharieva, Hristo Najdenski, Silviya Ruseva, Valentin Lozanov, Vessela Balabanova, Sakina Yagi, Georgi Momekov, Vanio Mitev
INTRODUCTION: Geigeria alata is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints. OBJECTIVE: To analyze phenolic acids in Geigeria alata roots and leaves and to evaluate their antioxidant and antimicrobial activities. METHODOLOGY: Phenolic acids in the aqueous-methanol extracts were identified by LC-MS. Major compounds were isolated using low-pressure liquid chromatography...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27880856/direct-analysis-in-real-time-mass-spectrometry-for-rapid-identification-of-traditional-chinese-medicines-with-coumarins-as-primary-characteristics
#19
Zhiyong Chen, Yuanyuan Yang, Hongxun Tao, Liping Liao, Ye Li, Zijia Zhang
INTRODUCTION: The increasing popularity of traditional Chinese medicines (TCMs) necessitates rapid and reliable methods for controlling their quality. Direct analysis in real-time mass spectrometry (DART-MS) represents a novel approach to analysing TCMs. OBJECTIVE: To develop a quick and reliable method of identifying TCMs with coumarins as primary characteristics. METHODOLOGY: DART-MS coupled with ion trap mass spectrometry was employed to rapidly identify TCMs with coumarins as primary characteristics and to explore the ionisation mechanisms of simple coumarins, furocoumarins and pyranocoumarins in detail...
November 23, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27976470/esi-ms-2-and-anti-inflammatory-studies-of-cyclopropanic-triterpenes-uplc-esi-ms-and-ms-2-search-of-related-metabolites-from-donella-ubanguiensis
#20
Louis P Sandjo, Marcus V P Dos Santos Nascimento, Layzon A L da Silva, Antonio C M Munhoz, Luiz A E Pollo, Maique W Biavatti, Bonaventure T Ngadjui, Till Opatz, Tania S Fröde
INTRODUCTION: Triterpenes are one of the largest secondary metabolites groups spread in the plant kingdom with various skeletons. These metabolites have showed various bioactivities including anti-inflammatory activity. OBJECTIVE: The study aims to explore the mass spectrometry fragmentation of donellanic acids A-C (DA A-C), three compounds identified from Donella ubanguiensis; in addition, the fragmentation behaviour of these metabolites will serve as a fingerprint to search and characterise triterpenes congeners in fruits, bark and wood crude extracts of D...
January 2017: Phytochemical Analysis: PCA
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