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Phytochemical Analysis: PCA

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https://www.readbyqxmd.com/read/28786149/quantitative-analysis-of-ingenol-in-euphorbia-species-via-validated-isotope-dilution-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#1
Tibor Béres, Klaus Dragull, Jiří Pospíšil, Danuše Tarkowská, Martin Dančák, Ondřej Bíba, Petr Tarkowski, Karel Doležal, Miroslav Strnad
INTRODUCTION: Various species of the Euphorbia genus contain diterpene ingenol and ingenol mebutate (ingenol-3-angelate), a substance found in the sap of the plant Euphorbia peplus and an inducer of cell death. A gel formulation of the drug has been approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for the topical treatment of actinic keratosis. OBJECTIVE: To develop a rapid and reliable method for quantification of ingenol in various plant extracts...
August 7, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28776774/identification-of-some-bioactive-metabolites-in-a-fractionated-methanol-extract-from-ipomoea-aquatica-aerial-parts-through-tlc-hplc-uplc-esi-qtof-ms-and-lc-spe-nmr-fingerprints-analyses
#2
Mahmoud Hefny Gad, Emmy Tuenter, Nagwa El-Sawi, Sabry Younes, El-Mewafy El-Ghadban, Kristiaan Demeyer, Luc Pieters, Yvan Vander Heyden, Debby Mangelings
INTRODUCTION: The plant species Ipomoea aquatica contains various bioactive constituents, e.g. phenols and flavonoids, which have several medical uses. All previous studies were executed in Asia; however, no reports are available from Africa, and the secondary metabolites of this plant species from Africa are still unknown. OBJECTIVE: The present study aims finding suitable conditions to identify the bioactive compounds from different fractions. METHODOLOGY: Chromatographic fingerprint profiles of different fractions were developed using high-performance liquid chromatography (HPLC) and then these conditions were transferred to thin-layer chromatography (TLC)...
August 4, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28766842/methanol-extracts-of-28-hieracium-species-from-the-balkan-peninsula-comparative-lc-ms-analysis-chemosystematic-evaluation-of-their-flavonoid-and-phenolic-acid-profiles-and-antioxidant-potentials
#3
Violeta Milutinović, Marjan Niketić, Ljuboš Ušjak, Dejan Nikolić, Aleksej Krunić, Christian Zidorn, Silvana Petrović
INTRODUCTION: Hieracium s. str. represents one of the largest and most complex genera of flowering plants. As molecular genetics seems unlikely to disentangle intricate relationships within this reticulate species complex, analysis of flavonoids and phenolic acids, known as good chemosystematic markers, promise to be more reliable. Data about pharmacological activity of Hieracium species are scarce. OBJECTIVE: Evaluation of the chemosystematic significance of flavonoids and phenolic acids of methanol extracts of aerial flowering parts of 28 Hieracium species from the Balkans...
August 2, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28741853/application-of-nir-spectroscopy-coupled-with-pls-regression-for-quantification-of-total-polyphenol-contents-from-the-fruit-and-aerial-parts-of-citrullus-colocynthis
#4
Tania S Rizvi, Fazal Mabood, Liaqat Ali, Mohammed Al-Broumi, Hamida K M Al Rabani, Javid Hussain, Farah Jabeen, Suryyia Manzoor, Ahmed Al-Harrasi
INTRODUCTION: Citrullus colocynthis (L.) Schrad is extensively used to treat diabetes, obesity, fever, cancer, amenorrhea, jaundice, leukemia, rheumatism, and respiratory diseases. Chemical studies have indicated the presence of several cucurbitacins, flavones, and other polyphenols in this plant. These phytochemical constituents are responsible for the interesting antioxidant and other biological activities of C. colocynthis. OBJECTIVE: In the present study, for the first time, near infrared (NIR) spectroscopy coupled with partial least square (PLS) regression analysis was used to quantify the polyphenolic phytochemicals of C...
July 25, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28741753/phytochemical-and-bioactive-potential-of-in-vivo-and-in-vitro-grown-plants-of-centaurea-ragusina-l-detection-of-dna-rna-active-compounds-in-plant-extracts-via-thermal-denaturation-and-circular-dichroism
#5
Valerija Vujčić, Sandra Radić Brkanac, Ivana Radojčić Redovniković, Siniša Ivanković, Ranko Stojković, Irena Žilić, Marijana Radić Stojković
INTRODUCTION: The phytochemical composition and biological activity of non-volatile components of Centaurea ragusina L. has not been studied previously. OBJECTIVES: Our aim was to evaluate the phytochemical and bioactive potential (including interactions with polynucleotides) of C. ragusina L. depending on the origin of plant material (in vivo - leaves from natural habitats, ex vitro - leaves from plants acclimated from culture media, in vitro - leaves and calli from plants grown in culture media) and polarity of solvents used in extract preparation (80 and 96% ethanol and water combinations or single solvents)...
July 25, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28722248/screening-of-hallucinogenic-compounds-and-genomic-characterisation-of-40-anatolian-salvia-species
#6
Seda Damla Hatipoglu, Burhanettin Yalcinkaya, Muslum Akgoz, Turan Ozturk, Ahmet C Goren, Gulacti Topcu
INTRODUCTION: Salvia, an important and widely available member of Lamiaceae family. Although comparative analysis on secondary metabolites in several Salvia species from Turkey has been reported, their hallucinogenic chemicals have not been screened thoroughly. OBJECTIVE: This study provides LC-MS/MS analysis of 40 Salvia species for screening their psychoactive constituents of salvinorin A and salvinorin B. 5S-rRNA gene non-coding region of Salvia plants was sequenced, aligned and compared with that sequence of Salvia divinorum plant...
July 19, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28722224/integrating-high-resolution-and-solid-state-magic-angle-spinning-nmr-spectroscopy-and-a-transcriptomic-analysis-of-soybean-tissues-in-response-to-water-deficiency
#7
Isabel D Coutinho, Tiago Bueno Moraes, Liliane Marcia Mertz-Henning, Alexandre Lima Nepomuceno, Willian Giordani, Juliana Marcolino-Gomes, Silvia Santagneli, Luiz Alberto Colnago
INTRODUCTION: Solid-state NMR (SSNMR) spectroscopy methods provide chemical environment and ultrastructural details that are not easily accessible by other non-destructive, high-resolution spectral techniques. High-resolution magic angle spinning (HR-MAS) has been widely used to obtain the metabolic profile of a heterogeneous sample, combining the resolution enhancement provided by MAS in SSNMR with the shimming and locking procedures in liquid-state NMR. OBJECTIVE: In this work, we explored the feasibility of using the HR-MAS and SSNMR techniques to identify metabolic changes in soybean leaves subjected to water-deficient conditions...
July 19, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28707355/supercritical-co2-extraction-of-lavandula-angustifolia-mill-flowers-optimisation-of-oxygenated-monoterpenes-coumarin-and-herniarin-content
#8
Igor Jerković, Maja Molnar, Senka Vidović, Jelena Vladić, Stela Jokić
INTRODUCTION: Lavandula angustifolia is good source of oxygenated monoterpenes containing coumarins as well, which are all soluble in supercritical CO2 (SC-CO2 ). OBJECTIVE: The study objective is to investigate SC-CO2 extraction parameters on: the total yield; GC-MS profile of the extracts; relative content of oxygenated monoterpenes; the amount of coumarin and herniarin; and to determine optimal SC-CO2 extraction conditions by response surface methodology (RSM)...
July 14, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28699693/isolation-of-three-triterpene-saponins-including-two-new-oleanane-derivatives-from-soldanella-alpina-and-hydrophilic-interaction-liquid-chromatography-evaporative-light-scattering-detection-of-these-three-saponins-in-four-soldenella-species
#9
Julia Haller, Stefan Schwaiger, Hermann Stuppner, Frank Gafner, Markus Ganzera
INTRODUCTION: The genus Soldanella is one of the few endemic to Europe. Some of its species have relevance in local traditional medicine. Earlier work has indicated the possible presence of saponins in S. alpina. OBJECTIVE: To investigate S. alpina and other related species for the occurrence of saponins. METHODS: Following sequential extraction with n-hexane, dichloromethane and ethyl acetate the subsequent methanolic extract of S. alpina roots was fractionated after solvent precipitation using fast centrifugal partition chromatography and column chromatography...
July 12, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28686312/flavonoid-composition-of-tarocco-citrus-sinensis-l-osbeck-clone-lempso-and-fast-antioxidant-activity-screening-by-dpph-uhplc-pda-it-tof
#10
Eduardo Sommella, Francesco Pagano, Giacomo Pepe, Carmine Ostacolo, Michele Manfra, Marcello Chieppa, Rosa Di Sanzo, Sonia Carabetta, Pietro Campiglia, Mariateresa Russo
INTRODUCTION: Clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone "Lempso" is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling. OBJECTIVE: To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method...
July 7, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28670816/liquid-chromatographic-method-for-the-determination-of-caffeoylquinic-acid-derivates-in-hieracium-pilosella-l
#11
Larisa Borisova-Jan, Dick Fransson, Per Claeson, Robert Burman
INTRODUCTION: Hieracium pilosella L. is a medicinal plant encountered in Europe in traditional herbal medicinal products. Caffeoylquinic (ortho-dihydroxycinnamic) acid derivatives are characteristic constituents used as analytical markers in the quality control of the herbal material. Until now, the caffeoylquinic acid derivatives have been assayed using a colorimetric method according to the French Pharmacopoeia. OBJECTIVE: To develop an HPLC-method for quantification of caffeoylquinic acid derivatives in H...
July 2, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28643853/qualitative-analysis-of-polyphenols-in-macroporous-resin-pretreated-pomegranate-husk-extract-by-hplc-qtof-ms
#12
Rahima Abdulla, Sanawar Mansur, Haizhong Lai, Ablikim Ubul, Guangying Sun, Guozheng Huang, Haji Akber Aisa
INTRODUCTION: Pomegranate (Punica granatum L.) husk is a traditional herbal medicine abundant in phenolic compounds and plays some roles in the treatment of oxidative stress, bacterial and viral infection, diabetes mellitus, and acute and chronic inflammation. OBJECTIVE: Identification and determination of polyphenols in macroporous resin pretreated pomegranate husk extract by high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF-MS)...
June 23, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28626912/structural-characterisation-of-acetogenins-from-annona-muricata-by-supercritical-fluid-chromatography-coupled-to-high-resolution-tandem-mass-spectrometry
#13
Laurent Laboureur, Natacha Bonneau, Pierre Champy, Alain Brunelle, David Touboul
INTRODUCTION: Acetogenins are plant polyketides known to be cytotoxic and proposed as antitumor candidates. They are also suspected to be alimentary neurotoxins. Their occurrence as complex mixtures renders their dereplication and structural identification difficult using liquid chromatography coupled to tandem mass spectrometry and efforts are required to improve the methodology. OBJECTIVE: To develop a supercritical fluid chromatography (SFC) high-resolution tandem mass spectrometry method, involving lithium post-column cationisation, for the structural characterisation of Annonaceous acetogenins in crude extracts...
June 19, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28612465/mass-spectrometry-imaging-of-low-molecular-weight-compounds-in-garlic-allium-sativum-l-with-gold-nanoparticle-enhanced-target
#14
Maria Misiorek, Justyna Sekuła, Tomasz Ruman
INTRODUCTION: Garlic (Allium sativum) is the subject of many studies due to its numerous beneficial properties. Although compounds of garlic have been studied by various analytical methods, their tissue distributions are still unclear. Mass spectrometry imaging (MSI) appears to be a very powerful tool for the identification of the localisation of compounds within a garlic clove. OBJECTIVE: Visualisation of the spatial distribution of garlic low-molecular weight compounds with nanoparticle-based MSI...
June 13, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28612431/phlorotannin-composition-of-laminaria-digitata
#15
Anne M Vissers, Augusta Caligiani, Stefano Sforza, Jean-Paul Vincken, Harry Gruppen
INTRODUCTION: Phlorotannins are complex mixtures of phloroglucinol oligomers connected via C-C (fucols) or C-O-C (phlorethols) linkages. Their uniformity in subunits and large molecular weight hamper their structural analysis. Despite its commercial relevance for alginate extraction, phlorotannins in Laminaria digitata have not been studied. OBJECTIVE: To obtain quantitative and structural information on phlorotannins in a methanolic extract from L. digitata. METHODOLOGY: The combined use of (13) C and (1) H NMR spectroscopy allowed characterisation of linkage types and extract purity...
June 13, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28612345/metabolome-comparison-of-bioactive-and-inactive-rhododendron-extracts-and-identification-of-an-antibacterial-cannabinoid-s-from-rhododendron-collettianum
#16
Inamullah Hakeem Said, Ahmed Rezk, Ishtiaq Hussain, Anne Grimbs, Abhinandan Shrestha, Hartwig Schepker, Klaudia Brix, Matthias S Ullrich, Nikolai Kuhnert
INTRODUCTION: The science of metabolomics offers the possibility to measure full secondary plant metabolomes with limited experimental effort to allow identification of metabolome differences using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) of liquid chromatography mass spectrometry (LC-MS) data. OBJECTIVE: To demonstrate a bioinformatics driven hypothesis generator for identification of biologically active compounds in plant crude extracts, which is validated by activity guided fractionation...
June 13, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28597993/one-step-extraction-and-hydrolysis-of-flavonoid-glycosides-in-rape-bee-pollen-based-on-soxhlet-assisted-matrix-solid-phase-dispersion
#17
Xijuan Tu, Shuangqin Ma, Zhaosheng Gao, Jing Wang, Shaokang Huang, Wenbin Chen
INTRODUCTION: Flavonoids are frequently found as glycosylated derivatives in plant materials. To determine contents of flavonoid aglycones in these matrices, procedures for the extraction and hydrolysis of flavonoid glycosides are required. The current sample preparation method is both labour and time consuming. OBJECTIVE: Develop a modified matrix solid phase dispersion (MSPD) procedure as an alternative methodology for the one-step extraction and hydrolysis of flavonoid glycosides...
June 9, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28589595/high-speed-counter-current-chromatography-with-an-online-storage-technique-for-the-preparative-isolation-and-purification-of-dihydroflavonoids-from-sophora-alopecuroides-l
#18
Zhong-Lin Sun, Jian-Ming He, Jiang-Er Lan, Qing Mu
INTRODUCTION: High-speed counter-current chromatography (HSCCC) is an efficient and non-absorption separation technique, but limitations still exist in simultaneous isolation of complex structures of natural products. Moreover, particular methods are various for different kinds of natural products. OBJECTIVE: A novel HSCCC strategy combined with an online storage recycling elution (OSR-CCC) technique was developed for the quick separation of naturally occurring dihydroflavonoids from the extract of the herb Sophora alopecuroides L...
June 6, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28543801/characterisation-of-pomegranate-husk-polyphenols-and-semi-preparative-fractionation-of-punicalagin
#19
Pedro Aguilar-Zárate, Jorge E Wong-Paz, Mariela Michel, Juan Buenrostro-Figueroa, Hugo R Díaz, Juan A Ascacio, Juan C Contreras-Esquivel, Gerardo Gutiérrez-Sánchez, Cristóbal N Aguilar
INTRODUCTION: Pomegranate-husk is the main by-product generated from the pomegranate industry. It is a potential source of compounds highly appreciated by different costumers. Punicalagin is the main compound present in pomegranate-husk. OBJECTIVE: To characterise the pomegranate-husk total polyphenols by HPLC-ESI-MS and to establish a method for the recovery of punicalagin using a medium pressure liquid chromatography (MPLC) system. MATERIALS AND METHODS: The characterisation of total pomegranate-husk polyphenols was carried out using liquid chromatography coupled to mass spectrometry...
May 23, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28523827/statistical-model-based-hplc-analytical-method-adjustment-strategy-to-adapt-to-different-sets-of-analytes-in-complicated-samples
#20
Binjun Yan, Xue Bai, Yunjie Sheng, Fanzhu Li
INTRODUCTION: On account of the complicated compositions of the products like traditional Chinese medicines (TCMs) and functional foods, it is a common practice to determine different sets of analytes in the same product for different purposes. OBJECTIVE: To efficiently develop the corresponding HPLC methods, a statistical model based analytical method adjustment (SMB-AMA) strategy was proposed. METHODS: In this strategy, the HPLC data acquired with design of experiments methodology were efficiently utilised to build the retention models for all the analytes and interferences shown in the chromatograms with multivariate statistical modelling methods...
May 19, 2017: Phytochemical Analysis: PCA
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