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Phytochemical Analysis: PCA

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https://www.readbyqxmd.com/read/30091158/important-issues-in-plant-tissues-analyses-by-hr-mas-nmr
#1
Igor S Flores, Bruno C B Martinelli, Vinicius S Pinto, Luiz H K Queiroz, Luciano M Lião
INTRODUCTION: High-resolution magic-angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy enables the analysis of the metabolic profile of plant and animal tissues under close to natural conditions, as well as of other heterogeneous natural or synthetic materials. Neither sample pretreatment is required after fragmentation nor powdering of the sample before insertion into the rotor. However, the efficiency of the method depends strongly on the sample preparation, rotor insertion procedure, and analysis conditions...
August 8, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/30033674/deep-eutectic-solvents-used-as-extraction-solvent-for-the-determination-of-flavonoids-from-camellia-oleifera-flowers-by-high-performance-liquid-chromatography
#2
Yaqian Ma, Menghan Liu, Ting Tan, Aiping Yan, Lan Guo, Kun Jiang, Changheng Tan, Yiqun Wan
INTRODUCTION: Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. OBJECTIVE: To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection...
July 23, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/30019471/profiling-of-hura-crepitans-l-latex-by-ultra-high-performance-liquid-chromatography-atmospheric-pressure-chemical-ionisation-linear-ion-trap-orbitrap-mass-spectrometry
#3
Manon Trinel, Valérie Jullian, Anne-Cécile Le Lamer, Icram Mhamdi, Kember Mejia, Denis Castillo, Billy Joel Cabanillas, Nicolas Fabre
INTRODUCTION: The phytochemistry of the latex of Hura crepitans L. (Euphorbiaceae), a widespread tree in the Amazonian forest having many uses, is little known. Only huratoxin, a daphnane diterpene orthoester, has been described despite the high pharmacological potential of this kind of compounds. Glucosphingolipids (cerebrosides) are also known to be distributed in Euphorbiaceae latexes. OBJECTIVE: To tentatively identify daphnanes diterpenes and cerebrosides in the latex of H...
July 17, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/30019400/volatile-compounds-in-different-parts-of-the-fruit-psidium-guajava-l-cv-media-china-identified-at-distinct-phenological-stages-using-hs-spme-gc-qtof-ms
#4
María P Elizalde-González, Erika J Segura-Rivera
INTRODUCTION: Determination of the volatile organic compounds (VOCs) emitted by immature fruits furthers our understanding of plant-pest interactions and by fruits in a ripe state concerns food quality. OBJECTIVES: To apply headspace solid-phase microextraction gas chromatography quadrupole time-of-flight mass spectrometry (HS-SPME-GC-QTOF/MS) to compare the volatiles emitted by different parts of guava (Psidium guajava L. cv. "Media China") at different maturation stages...
July 17, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29968391/potentially-toxic-pyrrolizidine-alkaloids-in-eupatorium-perfoliatum-and-three-related-species-implications-for-herbal-use-as-boneset
#5
Steven M Colegate, Roy Upton, Dale R Gardner, Kip E Panter, Joseph M Betz
INTRODUCTION: Pro-toxic dehydropyrrolizidine alkaloids are associated with liver disease in humans. The potential for long-term, low-level or intermittent exposures to cause or contribute to chronically-developing diseases is of international concern. Eupatorium perfoliatum is a medicinal herb referred to as boneset. While the presence of dehydropyrrolizidine alkaloids in some Eupatorium species is well-established, reports on Eupatorium perfoliatum are scant and contradictory. OBJECTIVE: To investigate the presence of dehydropyrrolizidine alkaloids in a survey of boneset samples and related alcoholic tinctures, and hot water infusions and decoctions...
July 2, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29882286/qualitative-and-quantitative-analysis-of-teucrium-polium-essential-oil-components-by-gc-ms-coupled-with-mcr-and-parafac-methods
#6
Hamidreza Nikpour, Mehdi Mousavi, Hamideh Asadollahzadeh
INTRODUCTION: Teucrium polium is a herbal plant which has curative potential and pharmacological characteristics. Therefore, establishment of accurate analytical methods for characterising its active components is of prime importance. OBJECTIVE: Because of the complex nature of essential oil samples, some results of direct gas chromatography-mass spectrometry (GC-MS) analysis maybe unreliable. Therefore, GC-MS is coupled with chemometric methods to resolve experimental information taken from chromatographic peaks, and assign compounds to them more correctly...
June 7, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29808594/uplc-esi-qtof-ms-2-characterisation-of-cola-nitida-resin-fractions-with-inhibitory-effects-on-no-and-tnf-%C3%AE-released-by-lps-activated-j774-macrophage-and-on-trypanosoma-cruzi-and-leishmania-amazonensis
#7
Larissa Frankenberger, Tamara D Mora, Carolina D de Siqueira, Fabiola B Filippin-Monteiro, Milene H de Moraes, Maique W Biavatti, Mario Steindel, Louis P Sandjo
INTRODUCTION: The resin of Cola nitida is used in western Cameroon as incense for spiritual protection and during ritual ceremonies. This plant secretion has never been investigated although previous chemical and biological studies on other resins have drawn many attentions. OBJECTIVE: The resin fractions which revealed inhibitory effect on nitric oxide (NO) and tumour necrosis factor alpha (TNF-α) released by lipopolysaccharide (LPS)-activated J774 macrophage as well as on intracellular forms of Leishmania amazonensis and Trypanosoma cruzi amastigote were chemically characterised...
May 29, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29808582/dereplication-of-plant-phenolics-using-a-mass-spectrometry-database-independent-method
#8
Ricardo M Borges, Rahil Taujale, Juliana Santana de Souza, Thaís de Andrade Bezerra, Eder Lana E Silva, Ronny Herzog, Francesca V Ponce, Jean-Luc Wolfender, Arthur S Edison
INTRODUCTION: Dereplication, an approach to sidestep the efforts involved in the isolation of known compounds, is generally accepted as being the first stage of novel discoveries in natural product research. It is based on metabolite profiling analysis of complex natural extracts. OBJECTIVE: To present the application of LipidXplorer for automatic targeted dereplication of phenolics in plant crude extracts based on direct infusion high-resolution tandem mass spectrometry data...
May 29, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29924474/advances-in-mass-spectrometry-techniques-applicable-to-phytochemical-analysis
#9
EDITORIAL
Ming-Quan Guo
No abstract text is available yet for this article.
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29785755/energy-resolved-mass-spectrometry-of-chlorogenic-acids-and-its-application-to-isomer-quantification-by-direct-infusion-tandem-mass-spectrometry
#10
Javier Hernandez, Anja Müller, Rakesh Jaiswal, Juan Z Davalos, Nikolai Kuhnert
INTRODUCTION: With the advent of high-perfomance liquid chromatography (HPLC)-tandem mass spectrometry (MS) using ion trap mass analysers it is possible to acquire unambigious structural information in particular with respect to aspects of regiochemistry and stereochemistry of organic compounds present in complex mixtures such as coffee extracts. However, HPLC-MS methods are resource extensive, laborious and lacking user friendliness. OBJECTIVE: To introduce a simple parameter - the energy threshhold for fragmentation - determined using energy resolved MS and demonstrate its value for the complete structural characterisation and even relative quantification of individual isomeric chlrogenic acids in direct infusion experiments...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29785715/current-advances-in-screening-for-bioactive-components-from-medicinal-plants-by-affinity-ultrafiltration-mass-spectrometry
#11
REVIEW
Guilin Chen, Bill X Huang, Mingquan Guo
INTRODUCTION: Medicinal plants have played an important role in maintaining human health for thousands of years. However, the interactions between the active components in medicinal plants and some certain biological targets during a disease are still unclear in most cases. OBJECTIVE: To conduct the high-throughput screening for small active molecules that can interact with biological targets, which is of great theoretical significance and practical value. METHODOLOGY: The ultrafiltration mass spectrometry (UF-LC/MS) is a powerful bio-analytical method by combining affinity ultrafiltration and liquid chromatography-mass spectrometry (LC/MS), which could rapidly screen and identify small active molecules that bind to biological targets of interest at the same time...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29687660/recent-development-in-mass-spectrometry-and-its-hyphenated-techniques-for-the-analysis-of-medicinal-plants
#12
REVIEW
Ming-Zhi Zhu, Gui-Lin Chen, Jian-Lin Wu, Na Li, Zhong-Hua Liu, Ming-Quan Guo
INTRODUCTION: Medicinal plants are gaining increasing attention worldwide due to their empirical therapeutic efficacy and being a huge natural compound pool for new drug discovery and development. The efficacy, safety and quality of medicinal plants are the main concerns, which are highly dependent on the comprehensive analysis of chemical components in the medicinal plants. With the advances in mass spectrometry (MS) techniques, comprehensive analysis and fast identification of complex phytochemical components have become feasible, and may meet the needs, for the analysis of medicinal plants...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29667236/recent-advances-in-matrix-assisted-laser-desorption-ionisation-mass-spectrometry-imaging-maldi-msi-for-in-situ-analysis-of-endogenous-molecules-in-plants
#13
REVIEW
Liang Qin, Yawen Zhang, Yaqin Liu, Huixin He, Manman Han, Yanyan Li, Maomao Zeng, Xiaodong Wang
INTRODUCTION: Mass spectrometry imaging (MSI) as a label-free and powerful imaging technique enables in situ evaluation of a tissue metabolome and/or proteome, becoming increasingly popular in the detection of plant endogenous molecules. OBJECTIVE: The characterisation of structure and spatial information of endogenous molecules in plants are both very important aspects to better understand the physiological mechanism of plant organism. METHODS: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a commonly-used tissue imaging technique, which requires matrix to assist in situ detection of a variety of molecules on the surface of a tissue section...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29603449/qualitative-and-quantitative-analysis-of-lipo-alkaloids-and-fatty-acids-in-aconitum-carmichaelii-using-lc-ms-and-gc-ms
#14
Ying Liang, Guan-Yu Yan, Jian-Lin Wu, Xingxing Zong, Zhongqiu Liu, Hua Zhou, Liang Liu, Na Li
INTRODUCTION: Lipo-alkaloid (LA) is a kind of C19 -norditerpenoid alkaloid in Aconitum species, which usually contains an aconitane skeleton and one or two fatty acid residues. OBJECTIVE: To qualify and quantify the fatty acids and lipo-alkaloids in Aconitum carmichaelii. METHODOLOGY: An ultra-high performance liquid chromatography-triple quadrupole-mass spectrometry (UHPLC-QQQ-MS) method was established to quantify LAs, while the free fatty acids were identified by gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UHPLC-Q-TOF-MS)...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29573482/screening-of-bioactive-components-from-traditional-chinese-medicines-using-cell-membrane-chromatography-coupled-with-mass-spectrometry
#15
REVIEW
Shengli Han, Yanni Lv, Fen Wei, Jia Fu, Qi Hu, Sicen Wang
INTRODUCTION: Cell membrane chromatography (CMC), as a highly selective type of affinity chromatography, has been demonstrated as an effective method to screen bioactive components acting on specific receptor from a complicated biological system. OBJECTIVE: To review the recent research progress and the technical applications of these analytical methods using CMC combined with gas chromatography-mass spectrometry, (GC/MS) and liquid chromatography-mass spectrometry (LC/MS)...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29573298/anti-ageing-effect-of-red-ginseng-revealed-by-urinary-metabonomics-using-rrlc-q-tof-ms
#16
Jinghui Sun, Chuanxin Jiao, Yue Ma, Jianguang Chen, Wei Wu, Shuying Liu
INTRODUCTION: Red ginseng (RG) is one of the main processed products of the roots and rhizomes of Panax ginseng C.A. Meyer and is used for anti-ageing. But how metabonomic influences of RG on the progress of ageing are less researched. OBJECTIVE: A metabonomic method was developed to study the characters of the ageing process and the effects of total ginsenosides of red ginseng (TGRG) on the progress of ageing. METHODS: Urine samples from four different ages (4, 12, 18 and 24 months old) of rats and interference after TGRG were analysed by rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) and multivariate statistical analysis were performed for the pattern recognition and characteristic metabolites identification...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29460310/lc-ms-based-metabolic-and-metabonomic-studies-of-panax-ginseng
#17
REVIEW
Wei Wu, Chuanxi Jiao, Hui Li, Yue Ma, Lili Jiao, Shuying Liu
INTRODUCTION: Panax ginseng has received much attention as a valuable health supplement with medicinal potential. Its chemical diversity and multiple pharmacological properties call for comprehensive methods to better understand the effects of ginseng and ginsenosides. Liquid chromatography-mass spectrometry (LC-MS) based metabonomic approaches just fit the purpose. OBJECTIVE: Aims to give a review of recent progress on LC-MS based pharmacokinetic, metabolic, and phytochemical metabolomic studies of ginseng, and metabonomic studies of ginseng intervention effects...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28895238/the-use-of-1-h-nmr-metabolomics-to-optimise-the-extraction-and-preliminary-identification-of-anthelmintic-products-from-the-leaves-of-lysiloma-latisiliquum
#18
Gloria Ivonne Hernández-Bolio, Erika Kutzner, Wolfgang Eisenreich, Juan Felipe de Jesús Torres-Acosta, Luis Manuel Peña-Rodríguez
INTRODUCTION: Tannin-rich forages are recognised as an important alternative for the control of gastrointestinal nematodes in small ruminants. Lysiloma latisiliquum, a forage commonly consumed by goats and sheep, has shown anthelmintic activity when tested against Haemonchus contortus. However, to date, the metabolites responsible for the activity are not known. OBJECTIVE: To use 1 H-NMR metabolomics in the extraction and identification of anthelmintic metabolites from L...
July 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29770982/characterisation-of-phenolic-compounds-by-uplc-qtof-ms-ms-of-geopropolis-from-the-stingless-bee-melipona-subnitida-janda%C3%A3-ra
#19
Silvana Alves de Souza, Telma Maria Guedes da Silva, Eva Monica Sarmento da Silva, Celso Amorim Camara, Tania Maria Sarmento Silva
INTRODUCTION: Melipona subnitida Ducke (jandaíra) is a stingless bee native to north-eastern Brazil, which produces geopropolis, a mixture of beeswax, plant resins, pollens and earth that is used for sealing beehives. OBJECTIVE: To extend the knowledge on phenolic compounds in fractions obtained by C18-solid phase extraction (SPE) of nine geopropolis samples from Melipona subnitida collected at different times. METHODOLOGY: Chromatographic profiles of nine samples of geopropolis from jandaíra were analysed by ultra-performance liquid chromatography coupled with a diode array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS/MS) and combined with the use of data-independent acquisition (MSE) for the profiling and structural characterisation of the phenolic compounds...
May 17, 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29732635/new-approach-for-fast-identification-of-cyclitols-by-maldi-tof-mass-spectrometry
#20
Hossam Al-Suod, Paweł Pomastowski, Magdalena Ligor, Viorica Railean-Plugaru, Bogusław Buszewski
INTRODUCTION: Alfalfa (Medicago sativa L.) is the subject of many studies due to its numerous chemical constituents and beneficial properties. Among these constituents are cyclitols, which have attracted attention due to the variety of biological properties they have. OBJECTIVE: A rapid and sensitive analytical procedure based on matrix-assisted laser desorption ionisation technique with time-of-flight and mass spectrometry (MALDI-TOF-MS) analysis was used for the first time for the identification of three cyclitols from different parts of alfalfa...
May 6, 2018: Phytochemical Analysis: PCA
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