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Phytochemical Analysis: PCA

Celia Rodríguez-Pérez, Gokhan Zengin, Antonio Segura-Carretero, Devina Lobine, Mohamad Fawzi Mahomoodally
INTRODUCTION: In the quest for new sources of biologically-active compounds, the chemical, and biological profiles of two Globularia species (G. trichosantha Fisch. & C. A. Mey and G. orientalis L.) were investigated. METHODOLOGY: Chemical profiles were evaluated by high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS), as well as by their total phenolic, flavonoids, and phenolic acids contents...
December 3, 2018: Phytochemical Analysis: PCA
Mingjuan Zhu, Pinqing Wei, Qian Peng, Shengying Qin, Yuan Zhou, Ren Zhang, Chenchen Zhu, Lei Zhang
INTRODUCTION: Coumarin and alkaloids are the major bioactive constituents of Toddalia asiatica, playing an important role in various biological activities such as anti-inflammatory, analgesic, anti-bacterial and anti-tumour. OBJECTIVE: To establish a method that will simultaneously determine the coumarins and alkaloids compounds in T. asiatica and identify their characteristic fragmentation patterns, while combining fingerprints and chemical identification with chemometrics for discrimination and quality assessment of T...
December 3, 2018: Phytochemical Analysis: PCA
Taewoong Rho, Soo-Jung Choi, Hyun Woo Kil, Jaeyoung Ko, Kee Dong Yoon
INTRODUCTION: Camellia japonica L. (Theaceae) is an evergreen shrub, which is cultivated as a popular ornamental tree in Korea, China, and Japan and its seeds have been used as a source of cooking oil, in cosmetics and as a traditional medicine. Intensive phytochemical works have revealed that oleanane-type saponins are the characteristic compounds of the seeds of C. japonica. OBJECTIVE: The purpose of the present study is to isolate and determine oleanane-type saponins from C...
November 26, 2018: Phytochemical Analysis: PCA
Laëtitia Fougère, David Da Silva, Emilie Destandau, Claire Elfakir
INTRODUCTION: Thin layer chromatography (TLC) is frequently used to obtain the fingerprint of a plant extract. Although the retardation factor and the response to visualisation give primary information about compound identification, the direct TLC-mass spectrometry (MS) coupling allows a more detailed characterisation of samples. OBJECTIVES: To demonstrate the potential for the flavonoid dereplication using an inorganic matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) method with and without TLC separation...
November 25, 2018: Phytochemical Analysis: PCA
Marzieh Piryaei, Mir Mehdi Abolghasemi, Babak Karimi
INTRODUCTION: Research on the volatile oil composition of Thymus kotschyanus Boiss was conducted by applying mesoporous organosilica based on alkylimidazolium ionic liquid (PMO-IL) as a fibre coating material via a method referred to as microwave-assisted headspace solid-phase microextraction (MA-HS-SPME). METHODOLOGY: This technique entails microwave irradiation of the sample and collection of the volatile sample components. These components are further introduced into a gas chromatography-mass spectrometry (GC-MS) injection port for further analysis...
November 20, 2018: Phytochemical Analysis: PCA
Sara Ramírez-Acosta, Ana Arias-Borrego, José Luis Gómez-Ariza, Tamara García-Barrera
INTRODUCTION: The storage of the vegetables products in a controlled atmosphere (CA) with low O2 and high CO2 concentrations, reduces respiration rates and delays the ripening process, and in some cases, improves their quality and organoleptic properties. OBJECTIVE: To obtain deep insight into strawberry fruit metabolic changes caused by these CA treatments. METHODOLOGY: Freshly harvested strawberries were preserved under different atmospheres enriched with 10%, 20% and 30% of CO2 , for 2 days at 0°C, containing in all the cases 5% of O2 and were subjected to a metabolomic analysis based on gas chromatography-mass spectrometry (GC-MS) and direct-infusion with electrospray ionisation source equipped with triple quadrupole coupled to time of flight mass spectrometry (DI-ESI-QqQ-TOF-MS)...
November 13, 2018: Phytochemical Analysis: PCA
Yongjing Liu, Hua Zhang, Hongmin Yu, Suhua Guo, Dawei Chen
INTRODUCTION: In the present study, a green and efficient extraction method using deep eutectic solvents as extraction solvent was developed for extracting the four major active compounds narirutin, naringin, hesperidin and neohesperidin from Aurantii Fructus. METHODOLOGY: A series of tunable deep eutectic solvents were prepared and investigated by mixing choline chloride or betaine to different hydrogen-bond donors, and betaine/ethanediol was found to be the most suitable extraction solvent...
November 13, 2018: Phytochemical Analysis: PCA
Alessandra Piovesana, Eliseu Rodrigues, Caciano Pelayo Zapata Noreña
INTRODUCTION: The hibiscus flower has received increasing interest because it contains high levels of bioactive compounds with remarkable functional properties. To the best of our knowledge, for the first time a detailed description of the carotenoid composition of hibiscus calyces is reported. OBJECTIVES: Identification and quantification of carotenoids, phenolic compounds and antioxidant activity from hibiscus calyces. MATERIAL AND METHODS: The composition of the carotenoids and phenolic compounds from hibiscus calyces was determined by high-performance liquid chromatography coupled to a diode array detector and tandem mass spectrometry (HPLC-DAD-MS/MS)...
November 13, 2018: Phytochemical Analysis: PCA
Joydeb Chanda, Pulok K Mukherjee, Rajarshi Biswas, Sayan Biswas, Amrendra Kumar Tiwari, Ashish Pargaonkar
INTRODUCTION: Luffa acutangula (L.) Roxb, commonly known as ridge gourd (cucurbitaceae), is a common vegetable cultivated in India. It is also a well-used medicinal plant in Indian traditional medicine. OBJECTIVES: To analyse the phenolics content of the most potent carbonic anhydrase-inhibiting fraction from an extract of L. acutangula. MATERIALS AND METHODS: An aqueous ethanol extract of dried fruits of L. acutangula was successively fractionated into petroleum ether, dichloromethane and ethyl acetate...
November 6, 2018: Phytochemical Analysis: PCA
Suguru Oguri, Kensuke Sakamaki, Hikaru Sakamoto, Kikue Kubota
INTRODUCTION: Flowering of the Asian skunk cabbage (Symplocarpus renifolius, Araceae) shows a sequential expression of female, bisexual and male sex phases. The protogynous thermogenic inflorescence has unpleasant odours, but the contributing chemical composition is poorly understood. OBJECTIVE: To determine the volatile composition of odour emissions from each S. renifolius flowering phase. METHODOLOGY: The dynamic headspace method was used to collect floral volatiles from six intact S...
October 31, 2018: Phytochemical Analysis: PCA
Vitor Soares, Rahil Taujale, Rafael Garrett, Antonio Jorge R da Silva, Ricardo M Borges
INTRODUCTION: Molecular networks are now established as the method of choice for tandem mass spectrometry dereplication and similarity-based structure elucidation. Node identification can be used to start the propagation of the structure elucidation of unknown compounds progressively. OBJECTIVE: To demonstrate the capabilities of using the LipidXplorer data results along with molecular networking to identify nodes and aid sequential structure elucidation of unknown compounds...
October 16, 2018: Phytochemical Analysis: PCA
Saidu Jibril, Norazah Basar, Hasnah Mohd Sirat, Roswanira Abdul Wahab, Naji Arafat Mahat, Lutfun Nahar, Satyajit D Sarker
INTRODUCTION: Cassia singueana Del. (Fabaceae) is a rare medicinal plant used in the traditional medicine preparations to treat various ailments. The root of C. singueana is a rich source of anthraquinones that possess anticancer, antibacterial and antifungal properties. OBJECTIVE: The objective of this study was to develop an ultrasound-assisted extraction (UAE) method for achieving a high extraction yield of anthraquinones using the response surface methodology (RSM), Box-Behnken design (BBD), and a recycling preparative high-performance liquid chromatography (HPLC) protocol for isolation of anthraquinones from C...
October 4, 2018: Phytochemical Analysis: PCA
Lucy Owen, Alex W White, Katie Laird
INTRODUCTION: The antimicrobial activity of many essential oils (EOs) is well established, indicating that EOs may be a source of compounds for antimicrobial drug development. Thin layer chromatography-direct bioautography (TLC-DB) can quickly identify antimicrobial components in complex mixtures and can be applied to the screening of EOs for lead compounds. OBJECTIVES: This study aimed to identify antimicrobial components of oregano, rosewood and cumin EOs against antibiotic-sensitive and -resistant bacteria using TLC-DB and a multi-faceted approach of GC-MS, LC-MS and NMR techniques to characterise bioactive compounds...
October 2, 2018: Phytochemical Analysis: PCA
Carlos Rial, Rosa M Varela, José M G Molinillo, Juan A López-Ráez, Francisco A Macías
INTRODUCTION: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10-10  M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical methods with very low limits of quantification. OBJECTIVE: To develop a sensitive and validated analytical method for the simultaneous quantification of seven SLs [7-oxoorobanchyl acetate (1), solanacol (2), orobanchol (4), strigol (5), fabacyl acetate (6), orobanchyl acetate (7), and 5-deoxystrigol (8)]...
October 2, 2018: Phytochemical Analysis: PCA
Hiroshi Kamitakahara, Tomoki Okayama, Praptiwi, Andria Agusta, Yuki Tobimatsu, Toshiyuki Takano
INTRODUCTION: The rhizome of Angiopteris evecta is of academic interest in Kalimantan, Indonesia, from an ethnobotanical perspective. Angiopteroside is a substance of pharmaceutical importance that is found in the rhizome of A. evecta. OBJECTIVE: The aims of this research are to improve the extraction method for angiopteroside from the rhizome, compared to that in a previous report, and to determine the yield of angiopteroside from the rhizome of A. evecta, as well as to obtain precise data for extractives from the rhizome of A...
September 25, 2018: Phytochemical Analysis: PCA
Yaqian Ma, Menghan Liu, Ting Tan, Aiping Yan, Lan Guo, Kun Jiang, Changheng Tan, Yiqun Wan
INTRODUCTION: Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. OBJECTIVE: To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection...
November 2018: Phytochemical Analysis: PCA
Manon Trinel, Valérie Jullian, Anne-Cécile Le Lamer, Icram Mhamdi, Kember Mejia, Denis Castillo, Billy Joel Cabanillas, Nicolas Fabre
INTRODUCTION: The phytochemistry of the latex of Hura crepitans L. (Euphorbiaceae), a widespread tree in the Amazonian forest having many uses, is little known. Only huratoxin, a daphnane diterpene orthoester, has been described despite the high pharmacological potential of this kind of compounds. Glucosphingolipids (cerebrosides) are also known to be distributed in Euphorbiaceae latexes. OBJECTIVE: To tentatively identify daphnanes diterpenes and cerebrosides in the latex of H...
November 2018: Phytochemical Analysis: PCA
María P Elizalde-González, Erika J Segura-Rivera
INTRODUCTION: Determination of the volatile organic compounds (VOCs) emitted by immature fruits furthers our understanding of plant-pest interactions and by fruits in a ripe state concerns food quality. OBJECTIVES: To apply headspace solid-phase microextraction gas chromatography quadrupole time-of-flight mass spectrometry (HS-SPME-GC-QTOF/MS) to compare the volatiles emitted by different parts of guava (Psidium guajava L. cv. "Media China") at different maturation stages...
November 2018: Phytochemical Analysis: PCA
Steven M Colegate, Roy Upton, Dale R Gardner, Kip E Panter, Joseph M Betz
INTRODUCTION: Pro-toxic dehydropyrrolizidine alkaloids are associated with liver disease in humans. The potential for long-term, low-level or intermittent exposures to cause or contribute to chronically-developing diseases is of international concern. Eupatorium perfoliatum is a medicinal herb referred to as boneset. While the presence of dehydropyrrolizidine alkaloids in some Eupatorium species is well-established, reports on Eupatorium perfoliatum are scant and contradictory. OBJECTIVE: To investigate the presence of dehydropyrrolizidine alkaloids in a survey of boneset samples and related alcoholic tinctures, and hot water infusions and decoctions...
November 2018: Phytochemical Analysis: PCA
Hamidreza Nikpour, Mehdi Mousavi, Hamideh Asadollahzadeh
INTRODUCTION: Teucrium polium is a herbal plant which has curative potential and pharmacological characteristics. Therefore, establishment of accurate analytical methods for characterising its active components is of prime importance. OBJECTIVE: Because of the complex nature of essential oil samples, some results of direct gas chromatography-mass spectrometry (GC-MS) analysis maybe unreliable. Therefore, GC-MS is coupled with chemometric methods to resolve experimental information taken from chromatographic peaks, and assign compounds to them more correctly...
November 2018: Phytochemical Analysis: PCA
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