journal
MENU ▼
Read by QxMD icon Read
search

Amino Acids

journal
https://www.readbyqxmd.com/read/28631012/rapid-preparation-of-bioluminescent-tracers-for-relaxin-family-peptides-using-sortase-catalysed-ligation
#1
Jia-Hui Wang, Xiao-Xia Shao, Meng-Jun Hu, Dian Wei, Wei-Han Nie, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo
Relaxin family is a group of peptide hormones with a variety of biological functions by activating G protein-coupled receptors RXFP1-4. We recently developed bioluminescent tracers for their receptor-binding assays by chemical conjugation with the ultrasensitive NanoLuc reporter. To simplify preparation of the bioluminescent tracers, in the present study, we established a sortase-catalysed ligation approach using the chimeric R3/I5 as a model. Following catalysis by recombinant sortase A, a NanoLuc reporter carrying the LPETG sortase recognition motif at the C-terminus was efficiently ligated to an R3/I5 peptide carrying four successive Gly residues at the A-chain N-terminus, via the formation of a peptide bond between the C-terminal LPET sequence of NanoLuc and the A-chain N-terminal Gly residue of R3/I5...
June 19, 2017: Amino Acids
https://www.readbyqxmd.com/read/28631011/isoprenyl-carboxyl-methyltransferase-inhibitors-a-brief-review-including-recent-patents
#2
REVIEW
Woo Seok Yang, Seung-Gu Yeo, Sungjae Yang, Kyung-Hee Kim, Byong Chul Yoo, Jae Youl Cho
Among the enzymes involved in the post-translational modification of Ras, isoprenyl carboxyl methyltransferase (ICMT) has been explored by a number of researchers as a significant enzyme controlling the activation of Ras. Indeed, inhibition of ICMT exhibited promising anti-cancer activity against various cancer cell lines. This paper reviews patents and research articles published between 2009 and 2016 that reported inhibitors of ICMT as potential chemotherapeutic agents targeting Ras-induced growth factor signaling...
June 19, 2017: Amino Acids
https://www.readbyqxmd.com/read/28624907/dityrosine-administration-induces-dysfunction-of-insulin-secretion-accompanied-by-diminished-thyroid-hormones-t3-function-in-pancreas-of-mice
#3
Yin-Yi Ding, Zhu-Qing Li, Xiang-Rong Cheng, Yu-Mei Ran, Sha-Ji Wu, Yonghui Shi, Guowei Le
Oxidized tyrosine products are commonly found in food with high protein content and have been demonstrated to cause damage of liver and kidney in our previous studies. Dityrosine (Dityr) is a typical oxidized tyrosine product. Due to its structural homology with thyroid hormones T3, we assumed that one of the endocrine systems most likely considered in connection with its disruption by Dityr may be the T3 action. T3 plays important roles in insulin synthesis, and thyroid hormone resistance (RTH) is associated with the impairment of glucose metabolism...
June 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28623466/increases-in-circulating-amino-acids-with-in-feed-antibiotics-correlated-with-gene-expression-of-intestinal-amino-acid-transporters-in-piglets
#4
Miao Yu, Chunlong Mu, Yuxiang Yang, Chuanjian Zhang, Yong Su, Zan Huang, Kaifan Yu, Weiyun Zhu
In-feed antibiotics have been commonly used to promote the growth performance of piglets. The antibiotics can increase protein utilization, but the underlying mechanism is largely unknown. The present study investigated the effects of in-feed antibiotics on intestinal AA transporters and receptors to test the hypothesis that the alteration of circulating AA profiles may be concomitant with the change of intestinal AA transporters and receptors. Sixteen litters of piglets at day 7 started to receive creep feed with (Antibiotic) or without (Control) antibiotic...
June 16, 2017: Amino Acids
https://www.readbyqxmd.com/read/28620737/analysis-of-bmaa-enantiomers-in-cycads-cyanobacteria-and-mammals-in-vivo-formation-and-toxicity-of-d-bmaa
#5
J S Metcalf, Doug Lobner, Sandra Anne Banack, Gregory A Cox, Peter B Nunn, Peter B Wyatt, Paul Alan Cox
Chronic dietary exposure to the cyanobacterial toxin β-N-methylamino-L-alanine (BMAA) triggers neuropathology in non-human primates, providing support for the theory that BMAA causes a fatal neurodegenerative illness among the indigenous Chamorro people of Guam. However, since there are two stereoisomers of BMAA, it is important to know if both can occur in nature, and if so, what role they might play in disease causation. As a first step, we analysed both BMAA enantiomers in cyanobacteria, cycads, and in mammals orally dosed with L-BMAA, to determine if enantiomeric changes could occur in vivo...
June 15, 2017: Amino Acids
https://www.readbyqxmd.com/read/28616751/roles-of-amino-acids-in-preventing-and-treating-intestinal-diseases-recent-studies-with-pig-models
#6
REVIEW
Yulan Liu, Xiuying Wang, Yongqing Hou, Yulong Yin, Yinsheng Qiu, Guoyao Wu, Chien-An Andy Hu
Animal models are needed to study and understand a human complex disease. Because of their similarities in anatomy, structure, physiology, and pathophysiology, the pig has proven its usefulness in studying human gastrointestinal diseases, such as inflammatory bowel disease, ischemia/reperfusion injury, diarrhea, and cancer. To understand the pathogenesis of these diseases, a number of experimental models generated in pigs are available, for example, through surgical manipulation, chemical induction, microbial infection, and genetic engineering...
June 14, 2017: Amino Acids
https://www.readbyqxmd.com/read/28603803/controlled-release-of-bsa-linked-cisplatin-through-a-pepgel-self-assembling-peptide-nanofiber-hydrogel-scaffold
#7
Jun Liang, Gang Liu, Jing Wang, Xiuzhi Susan Sun
Previously, it has been reported that a novel PepGel (h9e peptide) can be triggered into a solid physical hydrogel by the addition of selected ions and proteins for various biomedical applications. Moreover, PepGel displays shear-thinning and repeatedly reversible sol-gel transfer properties that enable it to be easily transferred via an injector. In this study, PepGel is proposed as a carrier for controlled releases of bovine serum albumin (BSA)-bound or -linked drugs. BSA-linked cisplatin (BSA-CP) is used as a model drug in this study and plays two roles: as a trigger of hydrogel and as a target drug for controlled release...
June 12, 2017: Amino Acids
https://www.readbyqxmd.com/read/28593346/the-first-identified-cathelicidin-from-tree-frogs-possesses-anti-inflammatory-and-partial-lps-neutralization-activities
#8
Lixian Mu, Lei Zhou, Juanjuan Yang, Li Zhuang, Jing Tang, Tong Liu, Jing Wu, Hailong Yang
As of February 2017, approximately 7639 amphibian species have been described in the AmphibiaWeb database. However, only 20 cathelicidin-like antimicrobial peptides have been identified to date from 10 amphibian species. Half of these peptides were identified from genome sequences and have not yet been functionally characterized. In this study, a novel cathelicidin-like peptide designated cathelicidin-PP was purified from the skin of tree frog Polypedates puerensis. Cathelicidin-PP is a 32 residue peptide of sequence ASENGKCNLLCLVKKKLRAVGNVIKTVVGKIA...
June 7, 2017: Amino Acids
https://www.readbyqxmd.com/read/28589394/single-and-chronic-l-serine-treatments-exert-antidepressant-like-effects-in-rats-possibly-by-different-means
#9
Mao Nagasawa, Tsuyoshi Otsuka, Yuki Togo, Masakazu Yamanaga, Junki Yoshida, Nobuo Uotsu, Sachiyuki Teramoto, Shinobu Yasuo, Mitsuhiro Furuse
In the present study, the effects of both single (6 mmol L-serine/10 ml/kg orally administrated) and chronic (2% L-serine solution freely given for 28 days) treatments on depression-like behavior were evaluated in Wistar rats, representing the control, and Wistar Kyoto rats, representing an animal model of depression. Both single and chronic L-serine treatments decreased the duration of immobility, which is an index of a depressive-like state, in the forced swimming test in both strains. However, the decreases in the duration of immobility appear to be regulated differently by the different mechanisms involved in single and chronic L-serine treatments...
June 6, 2017: Amino Acids
https://www.readbyqxmd.com/read/28578504/focus-on-o-phosphohydroxylysine-o-phosphohydroxyproline-n-1-phosphotryptophan-and-s-phosphocysteine
#10
REVIEW
Matthew J Piggott, Paul V Attwood
The synthesis and chemistry of the lesser-known phosphoamino acids, O-phosphohydroxylysine, O-phosphohydroxyproline, N (1)-phosphotryptophan and S-phosphocysteine are described in detail. In addition, where anything at all is known, the biological synthesis, occurrence and functions of these phosphoamino acids are described. Of these phosphoamino acids, only N (1)-phosphotryptophan has not been reported to occur in proteins; however, apart from the roles of S-phosphocysteine in the sugar transporter component (EII) and in catalysis by protein phosphotyrosine phosphatase, little is currently known about the biological roles of the phosphoamino acids when they occur as post-translational modifications...
June 3, 2017: Amino Acids
https://www.readbyqxmd.com/read/28573520/glycotriazole-peptides-derived-from-the-peptide-hsp1-synergistic-effect-of-triazole-and-saccharide-rings-on-the-antifungal-activity
#11
Eduardo F C Junior, Carlos F R C Guimarães, Lucas L Franco, Ricardo J Alves, Kelly C Kato, Helen R Martins, José D de Souza Filho, Marcelo P Bemquerer, Victor H O Munhoz, Jarbas M Resende, Rodrigo M Verly
This work proposes a strategy that uses solid-phase peptide synthesis associated with copper(I)-catalyzed azide alkyne cycloaddition reaction to promote the glycosylation of an antimicrobial peptide (HSP1) containing a carboxyamidated C-terminus (HSP1-NH2). Two glycotriazole-peptides, namely [p-Glc-trz-G(1)]HSP1-NH2 and [p-GlcNAc-trz-G(1)]HSP1-NH2, were prepared using per-O-acetylated azide derivatives of glucose and N-acetylglucosamine in the presence of copper(II) sulfate pentahydrate (CuSO4·5H2O) and sodium ascorbate as a reducing agent...
June 1, 2017: Amino Acids
https://www.readbyqxmd.com/read/28634827/highly-functionalized-cyclic-%C3%AE-amino-acid-moieties-as-promising-scaffolds-in-peptide-research-and-drug-design
#12
REVIEW
Loránd Kiss, István M Mándity, Ferenc Fülöp
Peptide-based drug research has received high attention in the field of medicinal chemistry over the past decade. For drug design, to improve proteolytic stability, it is desirable to include unnatural building blocks, such as conformationally restricted β-amino acid moieties, into the peptide sequence. Accordingly, the synthesis and incorporation of such conformationally rigid systems into novel type of peptides has gained large interest. Our research group has designed highly efficient methods for the construction of potential antimicrobial peptides...
May 30, 2017: Amino Acids
https://www.readbyqxmd.com/read/28555251/post-resistance-exercise-ingestion-of-milk-protein-attenuates-plasma-tnf%C3%AE-and-tnfr1-expression-on-monocyte-subpopulations
#13
Adam J Wells, Adam R Jajtner, Alyssa N Varanoske, David D Church, Adam M Gonzalez, Jeremy R Townsend, Carleigh H Boone, Kayla M Baker, Kyle S Beyer, Gerald T Mangine, Leonardo P Oliveira, David H Fukuda, Jeffrey R Stout, Jay R Hoffman
Attenuating TNFα/TNFr1 signaling in monocytes has been proposed as a means of mitigating inflammation. The purpose of this study was to examine the effects of a milk protein supplement on TNFα and monocyte TNFr1 expression. Ten resistance-trained men (24.7 ± 3.4 years; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of myoglobin; tumor necrosis factor-alpha (TNFα); and expression of tumor necrosis factor receptor 1 (TNFr1) on classical, intermediate, and non-classical monocytes...
May 29, 2017: Amino Acids
https://www.readbyqxmd.com/read/28540509/short-term-supplementation-of-isocaloric-meals-with-l-tryptophan-affects-pig-growth
#14
H N Liu, C-A A Hu, M M Bai, Gang Liu, M C B Tossou, K Xu, F N Li, P Liao, X F Kong, X Wu, Y L Yin
L-Tryptophan (Trp) and some of its metabolites regulate the circadian rhythm in mammals. We aimed to investigate the effects of short-term supplementation of Trp in isocaloric meals on growth performance using the parameters of multiple blood biomarkers and free amino acids in growing pigs. A total of 32 Landrace × Yorkshire barrows with a mean body weight of 8.64 (±1.13) kg were randomly assigned to four groups and then fed with various concentrations of Trp diets daily. Our results showed that sequential supplementation of different concentrations of Trp in isocaloric meals decreased the feed:gain (F:G) ratio (P = 0...
May 24, 2017: Amino Acids
https://www.readbyqxmd.com/read/28536844/evaluation-of-neurotensin-receptor-1-as-a-potential-imaging-target-in-pancreatic-ductal-adenocarcinoma
#15
Xiaoqin Yin, Mengzhe Wang, Hui Wang, Huaifu Deng, Tingting He, Yue Tan, Zehua Zhu, Zhanhong Wu, Shuo Hu, Zibo Li
Pancreatic cancer is one of the deadliest human malignancies and lack of effective diagnostic and therapeutic methods. Accumulating evidence suggests that the neurotensin (NT) and neurotensin receptors (NTRs) play key roles in pancreatic adenocarcinoma growth and survival. In this study, we not only evaluate the NTR1 expression in pancreatic cancer patient samples, but also explore the PET and fluorescence imaging of NTR1 expression in pancreatic cancer animal models. The NTR1 expression was evaluated by immunohistochemistry staining in clinical patient tissue samples with pancreatic ductal adenocarcinoma, insulinoma, and pancreatitis...
May 23, 2017: Amino Acids
https://www.readbyqxmd.com/read/28536843/human-interstitial-cellular-model-in-therapeutics-of-heart-valve-calcification
#16
REVIEW
Caimei He, Hai Tang, Zijian Mei, Nichujie Li, Zhi Zeng, Kwame Oteng Darko, Yulong Yin, Chien-An Andy Hu, Xiaoping Yang
Calcific aortic valve disease is a common, severe heart condition that is currently with no proven, effective drug treatment and requires a surgical valve replacement or an entire heart explanation. Thus, developing novel, targeted therapeutic approaches becomes a major goal for cardiovascular disease research. To achieve this goal, isolated heart valve interstitial cells could be an advanced model to explore molecular mechanisms and measure drug efficacy. Based on this progress, molecular mechanisms that harbor components of  inflammation and fibrosis coupled with proteins, for example, BMP-2, TLRs, RANKL, Osteoprotegerin, have been proposed...
May 23, 2017: Amino Acids
https://www.readbyqxmd.com/read/28534176/functions-of-pregnane-x-receptor-in-self-detoxification
#17
REVIEW
Liuqin He, Xihong Zhou, Niu Huang, Huan Li, Tiejun Li, Kang Yao, Yanan Tian, Chien-An Andy Hu, Yulong Yin
Pregnane X receptor (PXR, NR1I2), a member of the nuclear receptor superfamily, is a crucial regulator of nutrient metabolism and metabolic detoxification such as metabolic syndrome, xenobiotic metabolism, inflammatory responses, glucose, cholesterol and lipid metabolism, and endocrine homeostasis. Notably, much experimental and clinical evidence show that PXR senses xenobiotics and triggers the detoxification response to prevent diseases such as diabetes, obesity, intestinal inflammatory diseases and liver fibrosis...
May 22, 2017: Amino Acids
https://www.readbyqxmd.com/read/28523379/novel-dna-rna-targeting-amino-acid-beacon-for-the-versatile-incorporation-at-any-position-within-the-peptide-backbone
#18
Tamara Šmidlehner, Ivo Piantanida
One-pot tandem synthesis was for the first time applied to attach fluorophore to the amino acid side chain, yielding amino acid ready for peptide coupling at the N-terminus, and also upon activation at the C-terminus. Two new compounds differing only in fluorophore-linker length showed exceptional fluorimetric and CD recognition between DS-RNA and DS-DNA, thus being promising beacons for versatile peptide incorporation.
May 18, 2017: Amino Acids
https://www.readbyqxmd.com/read/28516269/%C3%AE-alanine-intercede-metabolic-recovery-for-amelioration-of-human-cervical-and-renal-tumors
#19
Muthuraman Pandurangan, Gansukh Enkhtaivan, Bhupendra Mistry, Rahul V Patel, Sohyun Moon, Doo Hwan Kim
β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody...
May 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28516268/gpna-inhibits-the-sodium-independent-transport-system-l-for-neutral-amino-acids
#20
Martina Chiu, Cosimo Sabino, Giuseppe Taurino, Massimiliano G Bianchi, Roberta Andreoli, Nicola Giuliani, Ovidio Bussolati
L-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system L transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system L transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine...
May 17, 2017: Amino Acids
journal
journal
30901
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"