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Cellular Physiology and Biochemistry

Peng-Cheng Kang, Kai-Ming Leng, Yue-Ping Liu, Yang Liu, Yi Xu, Wei Qin, Jian-Jun Gao, Zhi-Dong Wang, Sheng Tai, Xiang-Yu Zhong, Yun-Fu Cui
BACKGROUND/AIMS: Cholangiocarcinoma (CCA) is one of the most common malignant tumors of the biliary tract originating from biliary epithelial cells. Although many therapeutic strategies have been developed to treat CCA, the survival rate for CCA patients is still quite low. Thus it is urgent to elucidate the pathogenesis of CCA and to explore novel therapeutic targets. miR-191 has been shown to be associated with many human solid cancers, but the function of miR-191 in CCA is still poorly understood...
September 20, 2018: Cellular Physiology and Biochemistry
Chian-Jiun Liou, Ciao-Han Wei, Ya-Ling Chen, Ching-Yi Cheng, Chia-Ling Wang, Wen-Chung Huang
BACKGROUND/AIMS: Fisetin is a naturally abundant flavonoid isolated from various fruits and vegetables that was recently identified to have potential biological functions in improving allergic airway inflammation, as well as anti-oxidative and anti-tumor properties. Fisetin has also been demonstrated to have anti-obesity properties in mice. However, the effect of fisetin on nonalcoholic fatty liver disease (NAFLD) is still elusive. Thus, the present study evaluated whether fisetin improves hepatic steatosis in high-fat diet (HFD)-induced obese mice and regulates lipid metabolism of FL83B hepatocytes in vitro...
September 20, 2018: Cellular Physiology and Biochemistry
Hailin Zhang, Sha Chen, Meichun Zeng, Daopeng Lin, Yu Wang, Xunhang Wen, Changfu Xu, Li Yang, Xiaofang Fan, Yongsheng Gong, Hongyu Zhang, Xiaoxia Kong
BACKGROUND/AIMS: Acute lung injury (ALI) is induced by a variety of external and internal factors and leads to acute progressive respiratory failure. Previous studies have shown that apelin-13 can decrease the acute lung injury induced by LPS, but the specific mechanism is unclear. Therefore, a mouse lung injury model and a cell model were designed to explore the mechanism of how apelin-13 alleviates the acute lung injury caused by LPS. METHODS: The effect of apelin-13 on LPS-induced structural damage was determined by H&E staining and by the wet/dry weight ratio...
September 20, 2018: Cellular Physiology and Biochemistry
Chunyan Yao, Fengxia He, Longfei Liu, Zhiwei Zhang, Chenhui Zhao, Wen Qiu, Dan Zhao, Jing Zhang, Mengxiao Xie, Yajuan Gong, Tianyi Yu, Lu Xia, Baomei Qian, Yingwei Wang
BACKGROUND/AIMS: The activation of complement system and the formation of C5b-9 complex have been confirmed in the glomeruli of patients with mesangioproliferative glomerulonephritis (MsPGN). However, the role and mechanism of C5b-9-induced injury in glomerular mesangial cell (GMC) are poorly understood. Rat Thy-1N is an animal model for studying MsPGN. It has been revealed that the attack of C5b-9 to the GMC in rat Thy-1N is sublytic, and sublytic C5b-9 can cause GMC apoptosis, but the underlying mechanism is not fully elucidated...
September 20, 2018: Cellular Physiology and Biochemistry
Rong Li, Ming Li, Lin-Fang Zhang, Xiao-Ming Liu, Ting-Zi Hu, Xiu-Juan Xia, Jing-Shu Chi, Xiao-Xia Jiang, Can-Xia Xu
BACKGROUND/AIMS: Primary splenic angiosarcoma is an aggressive malignancy originating from endothelial cells with a particularly poor outcome despite radical therapy. Owing to its extremely low incidence, available data for splenic angiosarcoma are limited. The present study aimed to address this limitation by presenting a thorough retrospective analysis of Chinese primary splenic angiosarcoma patients over a 53-year period (1963-2016). METHODS: To determine the characteristics of Chinese primary splenic angiosarcoma and identify factors that impact the outcomes of this histology, we retrospectively retrieved reports of 110 Chinese primary splenic angiosarcoma cases published between 1963-2012...
September 20, 2018: Cellular Physiology and Biochemistry
Mashael R Al-Anazi, Nyla Nazir, Dilek Colak, Mohammed N Al-Ahdal, Ahmed A Al-Qahtani
BACKGROUND/AIMS: The hepatitis B virus X protein (HBx) is a viral trans-activator that plays a crucial role in pathogenesis of hepatocellular carcinoma (HCC) via an unknown mechanism. The role of HBx in modulating cell proliferation and programmed cell death is replete with controversies. Thus, the goal of this study was to elucidate the effect of HBx and its deletion mutants on cell cycle progression in human hepatoma cells. METHODS: Huh7 cells transfected with either full-length or truncated HBx were tested for their mitogenic potential based on their effect on the expression of key cell cycle-related proteins (p27, cyclin D1, p21, and p53) and pro-apoptotic proteins such as cleaved poly (ADP-ribose) polymerase (PARP) and Bax...
September 20, 2018: Cellular Physiology and Biochemistry
Xiaoye Fan, Lidong Wang, Jingbo Huang, Hongming Lv, Xuming Deng, Xinxin Ci
BACKGROUND/AIMS: Pterostilbene (Pts), a natural dimethylated analog of resveratrol from blueberries, exerts antioxidative and anti-apoptotic effects in various diseases. This study aims to investigate the protective effects and mechanism of Pts against acetaminophen (APAP)-induced hepatotoxicity in vivo. METHODS: C57BL/6 mice were treated with APAP or APAP+Pts. HepG2 cells were used to further explore the underlying mechanisms in vitro. The effects of Pts on hepatotoxicity were measured by commercial kits, Hematoxylin and Eosin (H&E) straining, TUNEL assay, Western blot analysis, and Flow cytometry assay...
September 20, 2018: Cellular Physiology and Biochemistry
Yaping Wang, Panpan Chen, Lihan Wang, Jing Zhao, Zhiwei Zhong, Yingchao Wang, Jifeng Xu
BACKGROUND/AIMS: Histone deacetylases (HDACs) play a critical role in the regulation of gene transcription, cardiac development, and diseases. The aim of this study was to investigate whether the inhibition of HDACs improves cardiac remodeling and its underlying mechanisms in a mouse myocardial infarction (MI) model. METHODS: The HDAC inhibitor trichostatin A (TSA, 0.1 mg/kg/day) was administered via daily intraperitoneal injections for 8 consecutive weeks after MI in C57/BL mice...
September 20, 2018: Cellular Physiology and Biochemistry
Hong Zhong, Mengting Liu, Yaya Ji, Minjuan Ma, Kun Chen, Tingming Liang, Chang Liu
BACKGROUND/AIMS: Liver damage is a typical manifestation of nonalcoholic fatty liver disease (NAFLD). It originates from excessive fat accumulation, leading to hepatocyte death, inflammation, and fibrosis. Nonalcoholic steatohepatitis (NASH) is a type of NAFLD with a prevalence of 49% in morbidly obese patients. Pyroptosis plays an important role in the development of NASH; thus, it is important to elucidate the effect of lipid accumulation on pyroptosis. Genipin (GNP), a natural water-soluble cross-linking agent, has hepatoprotective effects and decreases lipid accumulation in the liver; however, the mechanisms underlying these effects are unknown...
September 20, 2018: Cellular Physiology and Biochemistry
Hsin-Yuan Chen, Tsui-Chin Huang, Li-Chun Lin, Tzong-Ming Shieh, Chi-Hao Wu, Kai-Lee Wang, Yong-Han Hong, Shih-Min Hsia
BACKGROUND/AIMS: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effect of fucoidan on the growth of ULs activated by transforming growth factor beta (TGFβ). METHODS: We used ELT-3 (Eker rat leiomyoma tumor-derived cells) and HUtSMC (human uterine smooth muscle cells) as in vitro models...
September 20, 2018: Cellular Physiology and Biochemistry
Zhiyuan Zhang, Wenwei Qian, Sen Wang, Dongjian Ji, Qingyuan Wang, Jie Li, Wen Peng, Jiou Gu, Tao Hu, Bing Ji, Yue Zhang, Shijia Wang, Yueming Sun
BACKGROUND/AIMS: Long non-coding RNAs (lncRNAs) acting as competing endogenous RNAs (ceRNAs) play significant roles in the development of tumors, but the functions of specific lncRNAs and lncRNA-related ceRNA networks have not been fully elucidated for colon adenocarcinoma (COAD). In this study, we aimed to clarify the lncRNA-microRNA (miRNA)-mRNA ceRNA network and potential lncRNA biomarkers in COAD. METHODS: We extracted data from The Cancer Genome Atlas (TCGA) and identified COAD-specific mRNAs, miRNAs, and lncRNAs...
September 19, 2018: Cellular Physiology and Biochemistry
Jingjing Yang, Le Yang, Lei Tian, Xiaofang Ji, Lin Yang, Liying Li
BACKGROUND/AIMS: Macrophages, the most plastic cells in the haematopoietic system, are found in all tissues and show great functional heterogeneity. Sphingosine 1-phosphate (S1P)/ S1P receptors (S1PRs) system is widely involved in the process of inflammatory disease, whereas little evidence concerning its role in functional macrophage polarization is available. Thus, the present study was designed to evaluate the effects of S1P/S1PRs on functional polarization of macrophage in mouse bone marrow (BM)-derived monocyte/macrophages (BMMs)...
September 19, 2018: Cellular Physiology and Biochemistry
Jian Shi, Xiaohua Li, Fan Zhang, Linqun Kong, Xingyuan Zhang, Yu Cheng, Qinghai Guan, Xuefeng Cao, Wentao Zhu, Kun Ou, Qiangpu Chen, Sanyuan Hu
BACKGROUND & AIMS: Current studies have indicated that long non-coding RNAs (lncRNAs) could act as tumor biomarkers for disease diagnosis and prognosis prediction. In this study, we mainly focused on determining the expression of circulating lncRNAs in patients suffering for hilar cholangiocarcinoma (HC), aiming to reveal the potential lncRNA as a fingerprint. METHODS: A total 12 lncRNAs were previously proven to be aberrantly expressed in HC tumor tissues. All of the 12 lncRNAs were selected as candidate targets for subsequent circulating lncRNA assay...
September 19, 2018: Cellular Physiology and Biochemistry
Deng Yunlei, Fan Qiuling, Wang Xu, Zhao Qianwen, Cao Xu, Xu Li, Wang Lining
BACKGROUND/AIMS: Diabetic nephropathy is the one of the most serious microvascular complications of diabetes mellitus, and "metabolic memory" plays a vital role in the development of diabetic complications. To investigate the effect of epigenetics on metabolic memory, we analyzed the impact of transient high-glucose stimulation on the secretion of inflammatory factors from rat glomerular mesangial cells. METHODS: Rat glomerular mesangial cells (HBZY-1) were divided into three groups: high-glucose group (25 mM glucose), hypertonic group (5...
September 19, 2018: Cellular Physiology and Biochemistry
Hsiao-Chun Chen, Ming-Yen Chen, Ching-Liang Hsieh, Shu-Yih Wu, Hsin-Cheng Hsu, Yi-Wen Lin
BACKGROUND/AIMS: Acupuncture involves inserting a fine needle into a specific point, often called an acupoint, thereby initiating a therapeutic effect accompanied by phenomena such as soreness, heaviness, fullness, and numbness. Acupoints are characterized as points located in deep tissues with abundant sensory nerve terminals, which suggests that there is a strong relationship between acupoints and peripheral sensory afferents. In this study, we determined whether manual acupuncture (MA) or different frequencies of electroacupuncture (EA) share similar mechanisms for activating excitatory neurotransmission...
September 19, 2018: Cellular Physiology and Biochemistry
Juan Liu, Li Cui, Xinmin Yan, Xinyuan Zhao, Jinluo Cheng, Lei Zhou, Jianbo Gao, Zhong Cao, Xinhua Ye, Shen Hu
BACKGROUND/AIMS: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. METHODS: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR)...
September 19, 2018: Cellular Physiology and Biochemistry
Jie Li, Songlin Zhang, Meili Pei, Lei Wu, Yanli Liu, Huijin Li, Jiaojiao Lu, Xu Li
BACKGROUND/AIMS: Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. Snail1 has a pivotal role in the regulation of EMT, involving the loss of E-cadherin and concomitant upregulation of vimentin, among other biomarkers. We have found FSCN1 promoted EMT in ovarian cancer cells, but the precise mechanism of FSCN1 in EMT process has not been clearly elucidated. METHODS: The levels of FSCN1 and snail1 were determined in epithelial ovarian cancer(EOC) specimen and in ovarian cancer cells by RT-qPCR...
September 19, 2018: Cellular Physiology and Biochemistry
Rui Liu, Jia Peng, Huili Wang, Lei Li, Xiujie Wen, Yan Tan, Lin Zhang, Haoyuan Wan, Faming Chen, Xie Nie
BACKGROUND/AIMS: Nuclear factor erythroid 2-related factor 2 (Nrf2) is an oncogene in various types of cancers, including oral squamous cell carcinoma (OSCC). Oxysophocarpine (OSC) is a natural alkaloid that has multiple pharmacological activities. However, the biological functions and molecular mechanism underlying the effects of OSC on the growth and metastasis of OSCC are unclear. METHODS: Nrf2 levels were determined in OSCC tissues and non-cancerous specimens by quantitative real-time PCR, western blotting, and immunohistochemistry (IHC) assays...
September 19, 2018: Cellular Physiology and Biochemistry
Lin Song, Yongmin Huang, Xuandi Hou, Yaoheng Yang, Shashwati Kala, Zhihai Qiu, Rui Zhang, Lei Sun
BACKGROUND/AIMS: Sonodynamic therapy (SDT), based on the synergistic effect of low-intensity ultrasound and sonosensitizer, is a potential approach for non-invasive treatment of cancers. In SDT, mitochondria played a crucial role in cell fate determination. However, mitochondrial activities and their response to SDT remain elusive. The purpose of this study was to examine the response of mitochondria to SDT in tumor cells. METHODS: A human breast adenocarcinoma cell line - MCF-7 cells were subjected to 5-aminolevulinic acid (ALA)-SDT, with an average ultrasonic intensity of 0...
September 19, 2018: Cellular Physiology and Biochemistry
Yan Wang, Daoquan Dong, Shuqi Jiang, Enpu Zhang, Wenzhong Zheng, Longyi Mao, Weiqing Li, Jingcheng Zhou, Longlong Fan, Ran Cheng, Zesong Li, Zhengyu Fang, Yaoting Gui, Xianxin Li
BACKGROUND/AIMS: Increasing evidence has shown that miR-216b plays an important role in human cancer progression. However, little is known about the function of miR-216b in renal cell carcinoma. METHODS: The expression levels of miR-216b in renal cell carcinoma tissues and cell lines were examined by qRT-PCR. The biological role of miR-216b in renal cell carcinoma proliferation and/or metastasis was examined in vitro and in vivo. The target of miR-216b was identified by a dual-luciferase reporter assay...
September 19, 2018: Cellular Physiology and Biochemistry
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