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Cellular Physiology and Biochemistry

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https://www.readbyqxmd.com/read/27898421/stimulation-of-erythrocyte-cell-membrane-scrambling-by-quinine
#1
Morena Mischitelli, Mohamed Jemaà, Mustafa Almasry, Caterina Faggio, Florian Lang
BACKGROUND/AIMS: The analkaloid drug quinine is utilized mainly for the chemoprophylaxis of malaria. The multiple side effects of quinine include hemolytic anemia and hemolytic uremic syndrome, disorders involving suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Signaling contributing to stimulation of eryptosis include increase of cytosolic Ca2+ activity ([Ca2+]i), oxidative stress, ceramide and D4476 sensitive casein kinase...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898420/long-non-coding-rna-bancr-promotes-endometrial-cancer-cell-proliferation-and-invasion-by-regulating-mmp2-and-mmp1-via-erk-mapk-signaling-pathway
#2
Danni Wang, Danbo Wang, Ning Wang, Zaiqiu Long, Xuemei Ren
BACKGROUND/AIMS: Microarray screening had found BRAF-activated non-coding RNA (BANCR) was significantly upregulated in type 1 endometrial cancer (EC). This study aimed to assess the potential role of long non-coding RNA (lncRNA) BANCR in the pathogenesis and progression of type 1 EC. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to confirm the expression of BANCR in type 1 EC tissue, and analyze its clinical significance...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898411/radioprotective-effects-of-heat-killed-mycobacterium-tuberculosis-in-cultured-cells-and-radiosensitive-tissues
#3
Yuanyuan Chen, Yang Xu, Jicong Du, Jiaming Guo, Xiao Lei, Jianguo Cui, Cong Liu, Ying Cheng, Bailong Li, Fu Gao, Jintao Ju, Jianming Cai, Yanyong Yang
BACKGROUND: Exposure to ionizing radiation (IR) often causes severe damage to radiosensitive tissues, which limits the use of radiotherapy in cancer patients. Novel safe and effective radioprotectant is urgently required. It has been reported toll like receptor 2 (TLR2) plays a critical role in radioresistance. In this study, we demonstrated the protective effects of Heat-Killed Mycobacterium tuberculosis (HKMT), a potent TLR2 agonist, against IR. METHODS: Cell survival and apoptosis were determined by CCK-8 assay and Annexin V assay, respectively...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898410/cadmium-exposure-is-associated-with-the-prevalence-of-dyslipidemia
#4
Zhou Zhou, Yong-Hui Lu, Hui-Feng Pi, Peng Gao, Min Li, Lei Zhang, Li-Ping Pei, Xiang Mei, Lin Liu, Qi Zhao, Qi-Zhong Qin, Yu Chen, Yue-Ming Jiang, Zhao-Hui Zhang, Zheng-Ping Yu
BACKGROUND: Cadmium is a widespread environmental and occupational pollutant that accumulates in human body with a biological half-life exceeding 10 years. Cadmium exposure has been demonstrated to increase rates of cardiovascular diseases. Whether occupational cadmium exposure is associated with the increase in the prevalence of dyslipidemia and hence contributes to the risk of cardiovascular diseases is still equivocal. To test the hypothesis that exposure to cadmium is related to the prevalence of dyslipidemia, we examined the associations between blood cadmium concentration and the prevalence of dyslipidemia in workers occupationally exposed to cadmium in China...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898409/down-regulation-of-lncrna-dgcr5-correlates-with-poor-prognosis-in-hepatocellular-carcinoma
#5
Ruyi Huang, Xiaochen Wang, Wenjie Zhang, Guangyan Zhangyuan, Kangpeng Jin, Weiwei Yu, Yu Xie, Xiaoliang Xu, Hai Wang, Beicheng Sun
BACKGROUND/AIMS: Long non-coding RNAs (lncRNAs) have been reported to play pivotal roles in multiple tumors and can act as tumor biomarkers. In this study, we explored the association of the expression of an lncRNA, DGCR5 with clinicopathological features and prognosis in HCC. METHODS: Expression levels of DGCR5 were detected by quantitative real-time PCR (qRT-PCR) and the clinical data was obtained, including basic information, data of clinicopathology and cancer specific survival rate...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898408/sulodexide-protects-contrast-induced-nephropathy-in-sprague-dawley-rats
#6
Qing Zhao, Jianyong Yin, Zeyuan Lu, Yiwei Kong, Guangyuan Zhang, Binghui Zhao, Feng Wang
BACKGROUND: Sulodexide is a powerful antithrombin agent with reno-protective property. However, whether it has beneficial effects on Contrast-Induced Nephropathy (CIN) remained elusive. In the current study, we evaluated the therapeutic effects of Sulodexide on CIN and investigated the potential mechanisms. METHODS: CIN model was induced by intravenous injection of indomethacin, followed by Ioversol and L-NAME. Sprague-Dawley rats were divided into 4 groups: control group, CIN group, CIN+vehicle group (CIN rats pretreated with vehicle) and CIN+ Sulodexide (CIN rats pretreated with Sulodexide)...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898407/expression-pattern-and-regulatory-role-of-microrna-23a-in-conjugated-linoleic-acids-induced-apoptosis-of-adipocytes
#7
Renli Qi, Qi Wang, Jing Wang, Jinxiu Huang, Shan Jiang, Rong Xiao, Zuohua Liu, Feiyun Yang
BACKGROUND/AIMS: Conjugated linoleic acids (CLAs) are known to induce apoptosis in adipocytes; however, the cellular mechanisms involved remained illdefined. We explored the different apoptotic induction effects of two CLA isomers on adipocytes and then investigated the expression and function of microRNAs (miRNAs) related to the apoptosis. METHODS: TUNEL and FCM assays were used to detect CLAs-induced adipocyte apoptosis. Microarrays were used to compare the differential expression of miRNAs...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898405/n-acetyl-cysteine-attenuated-the-deleterious-effects-of-advanced-glycation-end-products-on-the-kidney-of-non-diabetic-rats
#8
Karina Thieme, Karolline S Da Silva, Nelly T Fabre, Sergio Catanozi, Maria Beatriz Monteiro, Daniele Pereira Santos-Bezerra, Juliana Martins Costa-Pessoa, Maria Oliveira-Souza, Ubiratan F Machado, Marisa Passarelli, Maria Lucia Correa-Giannella
AIM: To assess the renal effects of chronic exposure to advanced glycation end-products (AGEs) in the absence of diabetes and the potential impact of concomitant treatment with the antioxidant N-acetyl cysteine (NAC). METHODS: Wistar rats received intraperitoneally 20 mg/kg/day of albumin modified (AlbAGE) or not (AlbC) by advanced glycation for 12 weeks and oral NAC (600mg/L; AlbAGE+NAC and AlbC+NAC, respectively). Biochemical, urinary and renal morphological analyses; carboxymethyl-lysine (CML, an AGE), CD68 (macrophage infiltration), and 4-hydroxynonenal (4-HNE, marker of oxidative stress) immunostaining; intrarenal mRNA expression of genes belonging to pathways related to AGEs (Ager, Ddost, Nfkb1), renin-angiotensin system (Agt, Ren, Ace), fibrosis (Tgfb1, Col4a1), oxidative stress (Nox4, Txnip), and apoptosis (Bax, Bcl2); and reactive oxidative species (ROS) content were performed...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898404/prolonged-fasting-improves-endothelial-progenitor-cell-mediated-ischemic-angiogenesis-in-mice
#9
Bao Xin, Chun-Long Liu, Hong Yang, Cheng Peng, Xiao-Hui Dong, Chuan Zhang, Alex F Chen, He-Hui Xie
BACKGROUND/AIMS: Prolonged fasting (PF) was shown to be of great potency to promote optimal health and reduce the risk of many chronic diseases. This study sought to determine the effect of PF on the endothelial progenitor cell (EPC)-mediated angiogenesis in the ischemic brain and cerebral ischemic injury in mice. METHODS: Mice were subjected to PF or periodic PF after cerebral ischemia, and histological analysis and behavioral tests were performed. Mouse EPCs were isolated and examined, and the effects of EPC transplantation on cerebral ischemic injury were investigated in mice...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27898403/role-of-osteoprotegerin-opg-in-bone-marrow-adipogenesis
#10
Lili Zhang, Mengmeng Liu, Xiaokang Zhou, Yi Liu, Bo Jing, Xiaogang Wang, Qi Zhang, Yao Sun
BACKGROUND/AIMS: Bone marrow adipogenesis is one of the major characteristics of aged bone. Bone marrow mesenchymal stem cells (BMMSCs) prefer to differentiate into adipocytes instead of osteoblasts in the bone marrow cavity in aged hosts. The mechanism of formation and function of adipocytes in aged bone marrow needs further investigation. Osteoprotegerin (OPG) is a member of the tumor necrosis factor receptor (TNFR) super family, and it can inhibit the activities of osteoclasts. We found that adipocyte numbers increased in the bone marrow of Opg knockout mice...
November 30, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889776/cinnamic-acid-cinn-induces-apoptosis-and-proliferation-in-human-nasopharyngeal-carcinoma-cells
#11
Guangying Qi, Jian Chen, Changrong Shi, Yukun Wang, Sisi Mi, Wenhua Shao, Xiangyuan Yu, Yili Ma, Jiapu Ling, Jian Huang
BACKGROUND/AIMS: CINN is the main ingredient of the traditional Chinese medicine cinnamon. The purpose of the present study was to investigate the effects of CINN on the proliferation and apoptosis of NPC cells and to elucidate the underlying molecular mechanisms. MATERIALS AND METHODS: CNE2 human NPC cells were treated with various CINN concentrations. The effects of CINN on the proliferation and apoptosis of CNE2 NPC cells were examined using the MTT assay and flow cytometric analysis...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889774/stimulation-of-suicidal-erythrocyte-death-by-the-cdc25-inhibitor-nsc-95397
#12
Mohamed Jemaà, Morena Mischitelli, Myriam Fezai, Mustafa Almasry, Caterina Faggio, Florian Lang
BACKGROUND/AIMS: The CDC25B inhibitor NSC-95397 triggers apoptosis of tumor cells and is thus considered for the treatment of malignancy. The substance is effective in part by modification of gene expression. Similar to apoptosis of nucleated cells erythrocytes may undergo eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca2+ activity ([Ca2+]i), oxidative stress, ceramide, as well as activation of protein kinases...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889772/sonodynamic-therapy-inhibits-fibrogenesis-in-rat-cardiac-fibroblasts-induced-by-tgf-%C3%AE-1
#13
Yuanyuan Guo, Zengxiang Dong, Yuanqi Shi, Wei Wang, Lu Wang, Jing Sun, Xin Sun, Zhen Tian, Jianting Yao, Zhitao Li, Jiali Cheng, Ye Tian
BACKGROUND/AIMS: Sonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibrogenesis in rat cardiac fibroblasts. METHODS: The primary cardiac fibroblasts were isolated from rats, and induced to fibrogenesis with TGF-β1. With this in vitro model, we tested the preventive effects of SDT on fibrogenesis and further the underlying mechanism...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889771/cd44-and-cd44v6-are-correlated-with-gastric-cancer-progression-and-poor-patient-prognosis-evidence-from-42-studies
#14
Min Fang, Junrong Wu, Xin Lai, Huaying Ai, Yifeng Tao, Bo Zhu, Lingsha Huang
BACKGROUND/AIMS: The prognostic power of the levels of total CD44 and its isoform CD44v6 for patients with gastric cancer (GC) remains controversial. Therefore, our study aims to generalize the clinicopathological and prognostic significance of these two proteins in GC. METHODS: A literature search of the PubMed, Web of Science and Embase databases was conducted to identify eligible studies. The odds ratio (OR) with a 95% confidence interval (CI) was used to assess the effects...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889767/crocetin-inhibits-lipopolysaccharide-induced-inflammatory-response-in-human-umbilical-vein-endothelial-cells
#15
Lei Song, Chen Kang, Yuan Sun, Wenrui Huang, Wei Liu, Zhiyu Qian
BACKGROUND/AIM: Crocetin is a readily bioavailable and bioactive compound extracted from Saffron. Previous studies indicated its various biomedical properties including antioxidant and anti-coagulation potencies. However, its effect on inflammation, notably within the cardiovascular system, has not been investigated yet. In the present study, we utilized human umbilical vein endothelial cell (HUVEC) to elucidate the effect of Crocetin on vascular inflammation. METHODS: Cell viability and toxicity were evaluated by MTT and Lactate dehydrogenase (LDH) assay, respectively...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889765/contribution-of-common-variants-in-gabrg2-reln-and-nrg3-and-interaction-networks-to-the-risk-of-hirschsprung-disease
#16
Yang Wang, Jun Wang, Ying Zhou, Zhiyun Wei, Yongtao Xiao, Kejun Zhou, Jie Wen, Junkai Yan, Wei Cai
BACKGROUND: Hirschsprung disease (HSCR) is a complex and heterogeneous disorder, characterized by a deficit in enteric nervous system. Genome-wide studies implied GABRG2, RELN and NRG3 might be involved in HSCR etiology. Here, we aimed to assess genetic variants in GABRG2, RELN and NRG3 that may confer susceptibility to HSCR and explore genetic interaction networks in HSCR. METHODS: Using a strategy that combined case-control study and gene-gene interaction analysis with the MassArray system, we evaluated 24 polymorphisms within GABRG2, RELN and NRG3 in 104 HSCR cases and 151 normal controls of Han Chinese origin...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889764/high-uric-acid-activates-the-ros-ampk-pathway-impairs-cd68-expression-and-inhibits-oxldl-induced-foam-cell-formation-in-a-human-monocytic-cell-line-thp-1
#17
Chaohuan Luo, Xueke Lian, Liangli Hong, Jingfang Zou, Zhi Li, Yuzhang Zhu, Tianliang Huang, Yongneng Zhang, Yaqiu Hu, Huier Yuan, Tengfei Wen, Wanling Zhuang, Bozhi Cai, Xin Zhang, Ichiro Hisatome, Tetsuya Yamamoto, Jiexiong Huang, Jidong Cheng
BACKGROUND/AIMS: Hyperuricemia is part of the metabolic-syndrome cluster of abdominal obesity, impaired glucose tolerance, insulin resistance, dyslipidemia, and hypertension. Monocytes/macrophages are critical in the development of metabolic syndrome, including gout, obesity and atherosclerosis. However, how high uric acid (HUA) exposure affects monocyte/macrophage function remains unclear. In this study, we investigated the molecular mechanism of HUA exposure in monocytes/macrophages and its impact on oxidized low-density lipoprotein (oxLDL)-induced foam-cell formation in a human monocytic cell line, THP-1...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889763/inhibition-of-the-jak2-stat3-sosc1-signaling-pathway-improves-secretion-function-of-vascular-endothelial-cells-in-a-rat-model-of-pregnancy-induced-hypertension
#18
Jian-Ying Luo, Dan Fu, Ya-Qin Wu, Ying Gao
BACKGROUND/AIMS: The present study aimed to investigate the effects of the JAK2/STAT3/SOSC1 signaling pathway on the secretion function of vascular endothelial cells (VECs) in a rat model of pregnancy-induced hypertension (PIH). METHODS: A PIH rat model was established. Forty-eight pregnant Sprague-Dawley female rats were selected and assigned into four groups: the normal group (normal non-pregnant rats), the non-PIH group (pregnant rats without PIH), the PIH group (pregnant rats with PIH) and the AG490 group (pregnant rats with PIH treated with AG490)...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889762/evaluation-of-isolation-methods-for-circulating-tumor-cells-ctcs
#19
Galatea Kallergi, Eleni Politaki, Saad Alkahtani, Christos Stournaras, Vassilis Georgoulias
BACKGROUND: Detection of CTCs is a poor prognostic factor for many cancer types; however, their very low frequency represents an obstacle for their detection. The objective of the current study was to compare the performance of commonly used methods for CTCs isolation. METHODS: The evaluated methods using spiking experiments of MCF7, SKBR3 and MDA MB-231 breast cancer cell lines were (i) ficoll density gradient separation (DGS), (ii) red blood cell lysis (Erythrolysis) isolation, (iii) positive immunomagnetic selection (EpCAM Dynal beads), (iv) two different negative immunomagnetic separation systems (Dynal vs Miltenyi CD45 beads) as well as (v) the Cell Search platform and (vi) the ISET system...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889761/cilostazol-suppresses-il-23-production-in-human-dendritic-cells-via-an-ampk-dependent-pathway
#20
Quanxing Shi, Zhao Yin, Peilin Liu, Bei Zhao, Zhong Zhang, Shuai Mao, Tengfei Wei, Mengmeng Rao, Liguo Zhang, Shouli Wang
BACKGROUND/AIMS: Cilostazol has been previously demonstrated to inhibit IL-23 production in human synovial macrophages via a RhoA/ROCK-dependent pathway. However, whether cilostazol affects IL-23 production in human dendritic cells remains largely unknown. The present study was designed to investigate this question and elucidate the possible underlying mechanisms. METHODS: Human monocyte-derived dendritic cells (mo-DCs) were pretreated with or without cilostazol and then incubated with zymosan...
November 25, 2016: Cellular Physiology and Biochemistry
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