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Cellular Physiology and Biochemistry

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https://www.readbyqxmd.com/read/29788015/limb-bud-and-heart-attenuates-growth-and-invasion-of-human-lung-adenocarcinoma-cells-and-predicts-survival-outcome
#1
Mingming Deng, Ruoxi Yu, Shuo Wang, Ye Zhang, Zhi Li, Huicong Song, Bofang Liu, Ling Xu, Ximing Wang, Zhe Zhang, Qingjie Lv, Xiaonan Wang, Xiaofang Che, Xiujuan Qu, Yunpeng Liu, Xuejun Hu
BACKGROUND/AIMS: The transcription cofactor limb-bud and heart (LBH) is involved in embryonic development. However, its role in human lung cancer, especially lung adenocarcinoma (LUAD), remains unclear. METHODS: A public database and tissue microarray (TMA) were used to compare differences in LBH expression and its relationship with clinical characteristics. Tissue from an additional 70 LUAD patients with follow-up records was used to explore the correlation of LBH expression with prognosis...
May 18, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29788012/allograft-inflammatory-factor-1-mediates-macrophage-induced-impairment-of-insulin-signaling-in-adipocytes
#2
Jingqi Ren, Yaqiu Lin, Junni Tang, Hua Yue, Yanying Zhao
BACKGROUND/AIMS: Allograft inflammatory factor-1 (AIF-1) is an inflammatory cytokine produced mainly by macrophages within human white adipose tissue. Its expression is increased in obese subjects and positively correlated with insulin resistance. The purpose of this study is to characterize the regulatory role of AIF-1 in insulin signaling of adipocyte. METHODS: AIF-1 was over-expressed via transfection of AIF-1 cDNA into murine RAW 264.7 macrophages, and the constitutive expression of AIF-1 was decreased via transfection of targeting siRNA...
May 18, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29772575/therapeutic-effect-of-cd4-cd25-regulatory-t-cells-amplified-in-vitro-on-experimental-autoimmune-neuritis-in-rats
#3
Feng-Jie Wang, Dan Cui, Wei-Dong Qian
BACKGROUND/AIMS: This study aimed to explore whether the adoptive transfusion of autologous CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) has a therapeutic effect on Experimental autoimmune neuritis (EAN) model rats, and it provides new experimental and theoretical bases for the immunotherapy of Guillain-Barre syndrome (GBS). METHODS: CD4+CD25+ Tregs were sorted from the spleens of rats using immunomagnetic bead separation techniques combined with flow cytometry...
May 14, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29772566/the-epigenetic-factor-kdm2b-regulates-emt-and-small-gtpases-in-colon-tumor-cells
#4
Nefeli Zacharopoulou, Anna Tsapara, Galatea Kallergi, Evi Schmid, Saad Alkahtani, Saud Alarifi, Philip N Tsichlis, Sotirios C Kampranis, Christos Stournaras
BACKGROUND/AIMS: The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells. METHODS: We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins...
May 14, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768267/type-2-diabetes-promotes-cell-centrosome-amplification-via-akt-ros-dependent-signalling-of-rock1-and-14-3-3%C3%AF
#5
Pu Wang, Yu Cheng Lu, Jie Wang, Lan Wang, Hanry Yu, Yuan Fei Li, Alice Kong, Juliana Chan, Shaochin Lee
BACKGROUND/AIMS: Type 2 diabetes is associated with oxidative stress and DNA damage which can cause centrosome amplification. Thus, the study investigated centrosome amplification in type 2 diabetes and the underlying mechanisms. METHODS: Centrosome numbers in human peripheral blood mononuclear blood cells (PBMC) from healthy subjects and patients with type 2 diabetes were compared to access the association between type 2 diabetes and centrosome amplification. Colon cancer cells were used to investigate the molecular mechanisms underlying the centrosome amplification triggered by high glucose, insulin and palmitic acid...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768263/fibrinopeptide-a-induces-expression-of-c-reactive-protein-via-the-ros-erk1-2-p38-nf-%C3%AE%C2%BAb-signal-pathway-in-vascular-smooth-muscle-cells
#6
Shouzhu Xu, Jing Zhao, Juntian Liu, Wei Gou
BACKGROUND/AIMS: Atherosclerosis is a chronic inflammatory disease in the artery walls. Fibrinopeptide A (FPA) is a biomarker of the activation of coagulation system, and a high concentration of FPA in blood occurs in patients with ischemic heart disease etc. However, there exist few studies on the pathological effects of FPA in cardiovascular system. Therefore, the present study examined the effect of FPA on CRP expression in VSMCs and the molecular mechanisms. METHODS: mRNA and protein expression was identified by quantitative real-time PCR and Western blot, respectively...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768262/mir-598-suppresses-invasion-and-migration-by-negative-regulation-of-derlin-1-and-epithelial-mesenchymal-transition-in-non-small-cell-lung-cancer
#7
Fengming Yang, Ke Wei, Zhiqiang Qin, Weitao Liu, Chuchu Shao, Chaoshan Wang, Ling Ma, Mengyan Xie, Yongqian Shu, Hua Shen
BACKGROUND/AIMS: MicroRNAs regulate a wide range of biological processes of non-small cell lung cancer (NSCLC). Although miR-598 has been reported to act as a suppressor in osteosarcoma and colorectal cancer, the physiological function of miR-598 in NSCLC remains unknown. In this study, the role of miR-598 in NSCLC was investigated. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to estimate the expression of miR-598 and Derlin-1 (DERL1) in both NSCLC tissues and cell lines...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768261/n-cadherin-attenuates-high-glucose-induced-nucleus-pulposus-cell-senescence-through-regulation-of-the-ros-nf-%C3%AE%C2%BAb-pathway
#8
Gang Hou, Huiqing Zhao, Haijun Teng, Pei Li, Wenbin Xu, Junbin Zhang, Lulu Lv, Zhiliang Guo, Li Wei, Hui Yao, Yichun Xu
BACKGROUND/AIMS: Diabetes mellitus (DM) is a potential etiology of disc degeneration. N-cadherin (N-CDH) helps maintain the cell viability, cell phenotype and matrix biosynthesis of nucleus pulposus (NP) cells. Here, we mainly aimed to investigate whether N-CDH can attenuate high glucose-induced NP cell senescence and its potential mechanism. METHODS: Rat NP cells were cultured in a base culture medium and base culture medium with a 0.2 M glucose concentration. Recombinant lentiviral vectors were used to enhance N-CDH expression in NP cells...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768259/precise-intradermal-injection-of-nanofat-derived-stromal-cells-combined-with-platelet-rich-fibrin-improves-the-efficacy-of-facial-skin-rejuvenation
#9
Zhi-Jie Liang, Xiang Lu, De-Quan Li, Yi-Dan Liang, Dan-Dan Zhu, Fang-Xiao Wu, Xiao-Lin Yi, Ning He, Yan-Qing Huang, Chao Tang, Hong-Mian Li
BACKGROUND/AIMS: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768258/multiple-actions-of-rotenone-an-inhibitor-of-mitochondrial-respiratory-chain-on-ionic-currents-and-miniature-end-plate-potential-in-mouse-hippocampal-mhippoe-14-neurons
#10
Chin-Wei Huang, Kao-Min Lin, Te-Yu Hung, Yao-Chung Chuang, Sheng-Nan Wu
BACKGROUND/AIMS: Rotenone (Rot) is known to suppress the activity of complex I in the mitochondrial chain reaction; however, whether this compound has effects on ion currents in neurons remains largely unexplored. METHODS: With the aid of patch-clamp technology and simulation modeling, the effects of Rot on membrane ion currents present in mHippoE-14 cells were investigated. RESULTS: Addition of Rot produced an inhibitory action on the peak amplitude of INa with an IC50 value of 39...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768257/systemic-immune-inflammation-index-sii-is-useful-to-predict-survival-outcomes-in-patients-after-liver-transplantation-for-hepatocellular-carcinoma-within-hangzhou-criteria
#11
Hongyuan Fu, Jun Zheng, Jianye Cai, Kaining Zeng, Jia Yao, Liang Chen, Hui Li, Jiebin Zhang, Yingcai Zhang, Hui Zhao, Yang Yang
BACKGROUND: There is growing evidence that the systemic immune-inflammation index (SII), a novel prognostic biomarker based on peripheral lymphocyte, neutrophil, and platelet counts, is associated with poor prognosis for several tumors. However, the prognostic value of SII in patients with hepatocellular carcinoma (HCC) who undergo liver transplantation (LT) remains unclear. The aim of this study was to determine the correlation between SII and prognosis in these patients. METHODS: This retrospective study involved 150 patients with HCC who underwent LT within the Hangzhou criteria...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768256/overexpression-of-pck1-gene-antagonizes-hepatocellular-carcinoma-through-the-activation-of-gluconeogenesis-and-suppression-of-glycolysis-pathways
#12
Yufu Tang, Yibing Zhang, Chunhui Wang, Zhongyi Sun, Longfei Li, Shuqun Cheng, Wenping Zhou
BACKGROUND/AIMS: Gluconeogenesis, a reverse process of glycolysis, is suppressed in neoplastic livers. Cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C/PCK1, encoded by PCK1) is a step limiting enzyme of gluconeogenesis. The induced expression of the factor is reported to initiate gluconeogenesis process and antagonize hepatocellular carcinoma (HCC). In the current study, the effect of the modulation of PCK1 expression on HCC was assessed. METHODS: The levels of PCK1 in clinical HCC tissues and different HCC cell lines were investigated with real time quantitative PCR, immunochemistry, and western blotting...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768255/asiatic-acid-aa-sensitizes-multidrug-resistant-human-lung-adenocarcinoma-a549-ddp-cells-to-cisplatin-ddp-via-downregulation-of-p-glycoprotein-mdr1-and-its-targets
#13
Qilai Cheng, Meixiang Liao, Haibo Hu, Hongliang Li, Longhuo Wu
BACKGROUND/AIMS: P-glycoprotein (P-gp, i.e., MDR1) is associated with the phenotype of multidrug resistance (MDR) and causes chemotherapy failure in the management of cancers. Searching for effective MDR modulators and combining them with anticancer drugs is a promising strategy against MDR. Asiatic acid (AA), a natural triterpene isolated from the plant Centella asiatica, may have an antitumor activity. The present study assessed the reversing effect of AA on MDR and possible molecular mechanisms of AA action in MDR1-overexpressing cisplatin (DDP)-resistant lung cancer cells, A549/DDP...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29768254/neuroprotection-against-hypoxic-ischemic-injury-%C3%AE-opioid-receptors-and-bdnf-trkb-pathway
#14
Shiying Sheng, Jingzhong Huang, Yi Ren, Feng Zhi, Xuansong Tian, Guoqiang Wen, Guanghong Ding, Terry C Xia, Fei Hua, Ying Xia
The delta-opioid receptor (DOR) is one of three classic opioid receptors in the opioid system. It was traditionally thought to be primarily involved in modulating the transmission of messages along pain signaling pathway. Although there were scattered studies on its other neural functions, inconsistent results and contradicting conclusions were found in past literatures, especially in terms of DOR's role in a hypoxic/ischemic brain. Taking inspiration from the finding that the turtle brain exhibits a higher DOR density and greater tolerance to hypoxic/ischemic insult than the mammalian brain, we clarified DOR's specific role in the brain against hypoxic/ischemic injury and reconciled previous controversies in this aspect...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763931/a-functional-variant-rs35592567-in-tp63-at-3q28-is-associated-with-gastric-cancer-risk-via-modifying-its-regulation-by-microrna-140
#15
Chaomin Lu, Yongwei Yang, Siyue Ma
BACKGROUND/AIMS: TP63 was believed to play an important role in the development of many malignancies, while the polymorphisms located at the miRNA binding sites within the 3'UTR of TP63 mRNA may interfere with its expression. In this study, we aimed to study the role of TP63 regulation in the tumorigenesis of gastric cancer (GC). METHODS: Computational and luciferase analysis were used to search and confirm the target of miR-140. Real-time PCR, western-blot, MTT assay, and flow cytometry cell cycle analysis were utilized to explore the molecular pathway of miR-140 involved in the progression of GC...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763928/identification-and-verification-of-candidate-genes-regulating-neural-stem-cells-behavior-under-hypoxia
#16
Zhongju Shi, Zhijian Wei, Jiahe Li, Shiyang Yuan, Bin Pan, Fujiang Cao, Hengxing Zhou, Yan Zhang, Yao Wang, Shiwei Sun, Xiaohong Kong, Shiqing Feng
BACKGROUND/AIMS: Neural stem cells (NSCs) reside in a hypoxic environment, and hypoxia plays an important role in their development and differentiation. This study aimed to explore the underlying mechanisms by which hypoxia affects NSC behavior. METHODS: In the current study, we downloaded the gene expression dataset GSE68572 and identified the differentially expressed genes (DEGs) by analyzing high-throughput gene expression in hypoxic and normoxic NSCs. Subsequently, we analyzed these data using a combined bioinformatics approach and predicted the microRNAs (miRNAs) targeting the key gene using miRNA databases...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763919/inhalation-of-hydrogen-of-different-concentrations-ameliorates-spinal-cord-injury-in-mice-by-protecting-spinal-cord-neurons-from-apoptosis-oxidative-injury-and-mitochondrial-structure-damages
#17
Xiao Chen, Jin Cui, Xiao Zhai, Jun Zhang, Zhengrong Gu, Xin Zhi, Weizong Weng, Panpan Pan, Liehu Cao, Fang Ji, Zhiwei Wang, Jiacan Su
BACKGROUND/AIMS: Hydrogen selectively neutralizes reactive oxygen species (ROS) and ameliorates various ROS-induced injuries. Spinal cord injury (SCI) is a serious injury to the central nervous system, and secondary SCI is closely related to excessive ROS generation. We hypothesized that hydrogen inhalation ameliorates SCI, and the mechanism of action may be related to the protective effects of hydrogen against oxidative stress, apoptosis, and mitochondrial damage. METHODS: Mechanically injured spinal cord neurons were incubated with different concentrations of hydrogen in vitro...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763918/comprehensive-effects-of-suppression-of-microrna-383-in-human-bone-marrow-derived-mesenchymal-stem-cells-on-treating-spinal-cord-injury
#18
Guo-Jun Wei, Ke-Wen Zheng, Gang An, Zuo-Wei Shi, Kai-Fu Wang, Ying Guan, Yan-Song Wang, Peng-Fei Li, Da-Ming Dong
BACKGROUND/AIMS: Transplantation of bone-marrow-derived mesenchymal stem cells (MSCs) promotes neural cell regeneration after spinal cord injury (SCI). Recently, we showed that suppression of microRNA-383 (miR-383) in MSCs increased the protein levels of glial cell line derived neurotrophic factor (GDNF), resulting in improved therapeutic effects on SCI. However, the overall effects of miR-383 suppression in MSCs on SCI therapy were not determined yet. Here, we addressed this question...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763909/cardiac-ablation-of-socs3-aggravates-doca-salt-induced-hypertrophic-remodeling-by-activation-of-gp130-dependent-signaling-in-mice
#19
Shuang Liu, Li-Xin Liu, Yun-Long Zhang, Song Lai, Yun-Peng Xie, Nan-Nan Li, Hong-Xia Wang, Yun-Long Xia, Ying Liu, Hui-Hua Li
BACKGROUND/AIMS: Cardiac remodeling is a critical pathogenetic process leading to heart failure. Suppressor of cytokine signaling-3 (SOCS3) is demonstrated as a key negative regulator of the gp130 receptor to inhibit cardiac hypertrophy. However, the role of SOCS3 in deoxycorticosterone-acetate (DOCA)-salt-induced cardiac remodeling remains unclear. METHODS: Cardiac-specific SOCS3 knockout (SOCS3cKO) and wild-type (WT) C57BL/6J mice were subjected to uninephrectomy and DOCA-salt for 3 weeks...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29763908/connexin-43-modulates-osteogenic-differentiation-of-bone-marrow-stromal-cells-through-gsk-3beta-beta-catenin-signaling-pathways
#20
Fei-Xiang Lin, Gui-Zhou Zheng, Bo Chang, Rong-Chun Chen, Qi-Hao Zhang, Peng Xie, Da Xie, Guo-Yong Yu, Qin-Xiao Hu, De-Zhong Liu, Shi-Xin Du, Xue-Dong Li
BACKGROUND/AIMS: Bone marrow stromal cells (BMSCs) are multipotent precursors that give rise to osteoblasts, and contribute directly to bone formation. Connexin 43 (Cx43) is the most ubiquitous gap junction protein expressed in bone cell types, and plays crucial roles in regulating intercellular signal transmission for bone development, differentiation and pathology. However, the precise role and mechanism of Cx43 in BMSCs are less known. Here, we investigate the function of Cx43 in osteogenic differentiation of BMSCs in vitro...
May 10, 2018: Cellular Physiology and Biochemistry
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