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Cellular Physiology and Biochemistry

Chenlei Zhu, Dong Cheng, Xubin Qiu, Ming Zhuang, Zhiwei Liu
BACKGROUND/AIMS: Long noncoding RNAs (lncRNAs) have been a research hotspot, as they play important roles in tumor development. However, their expression pattern and biological function in osteosarcoma have not yet been clarified. METHODS: Differentially expressed lncRNAs in osteosarcoma and paracarcinoma tissues were identified by screening an lncRNA microarray, and candidate lncRNAs were verified by quantitative real-time PCR (qRT-PCR). A series of bioinformatics and molecular biological methods were adopted to investigate the interaction among lncRNA, microRNA (miRNA), and miRNA target genes during the development and occurrence of osteosarcoma...
November 19, 2018: Cellular Physiology and Biochemistry
Chenxing Zhang, Chenyue Zhang, Jiamao Lin, Haiyong Wang
BACKGROUND/AIMS: An increasing number of studies have suggested that circular RNAs (circRNAs) have vital roles in carcinogenesis and tumor progression. However, the function of circRNAs in hepatocellular carcinoma (HCC) remains poorly characterized. METHODS: We investigated the levels of circRNAs in patients with HCC to identify potential diagnostic biomarkers. We examined circRNA expression profiles in liver tumors and paired non-cancerous liver tissues from three HCC patients with cancer thrombus using a circRNA microarray...
November 19, 2018: Cellular Physiology and Biochemistry
Maria A Usik, Irina V Ogneva
BACKGROUND/AIMS: Changes in the external mechanical field result in cytoskeleton reorganization and the formation of adaptive patterns in different types of cells, including somatic cells and sex cells. The aim of this research was to study the protein and mRNA content of cytoskeletal and sperm-specific genes in the sperm and testis cells of mice. METHODS: Mice were subjected to 30 days of antiorthostatic suspension to simulate weightlessness, followed by 12 h of recovery, while receiving essential phospholipids at a dosage of 500 mg/kg/day (30HSE and 30HSE+12h groups) or a similar dosage of a placebo (30HS and 30HS+12h groups)...
November 19, 2018: Cellular Physiology and Biochemistry
Fangwei Li, Jian Wang, Yanting Zhu, Lu Liu, Wei Feng, Wenhua Shi, Qingting Wang, Qianqian Zhang, Limin Chai, Manxiang Li
BACKGROUND/AIMS: The underlying molecular mechanisms involved in sphingosine kinase 1 (SphK1)/sphingosine 1-phosphate (S1P) mediation of platelet-derived growth factor (PDGF)-induced pulmonary arterial smooth muscle cell (PASMC) proliferation are still unclear, and the present study aims to address this issue. METHODS: Small interfering RNA (siRNA) and microRNA inhibitor transfection was performed to block the expression of SphK1, bone morphogenetic protein receptor II (BMPRII) and microRNA-21 (miR-21)...
November 19, 2018: Cellular Physiology and Biochemistry
Yang Wu, Tan Yuan, Wei-Wei Wang, Peng-Lei Ge, Zhi-Qiang Gao, Gong Zhang, Zhe Tang, Xiao-Wei Dang, Yong-Fu Zhao, Jian-Ying Zhang, Guo-Zhong Jiang
BACKGROUND/AIMS: This study aims to examine the effect of long noncoding RNA HOST2 (LncRNA HOST2) on epithelial-mesenchymal transition (EMT), proliferation, invasion and migration of hepatocellular carcinoma (HCC) cells via activation of the JAK2-STAT3 signaling pathway. METHODS: HCC and para-cancerous tissues were collected from 136 HCC patients. Immunohistochemistry was used to detect the expression of JAK2 and STAT3. HCC SMMC7721 cells were grouped into blank, negative control (NC), HOST2 mimic and HOST2 inhibitor groups...
November 19, 2018: Cellular Physiology and Biochemistry
Patrycja Rachubik, Maria Szrejder, Dorota Rogacka, Irena Audzeyenka, Michał Rychłowski, Stefan Angielski, Agnieszka Piwkowska
BACKGROUND/AIMS: Podocytes are dynamic polarized cells on the surface of glomerular capillaries that are an essential part of the glomerular filtration barrier. AMP-activated protein kinase (AMPK), a key regulator of glucose and fatty acid metabolism, plays a major role in obesity and type 2 diabetes. Accumulating evidence suggests that TRPC6 channels are crucial mediators of calcium transport in podocytes and are involved in regulating glomerular filtration. Here we investigated whether the AMPK-TRPC6 pathway is involved in insulin-dependent cytoskeleton reorganization and glucose uptake in cultured rat podocytes...
November 19, 2018: Cellular Physiology and Biochemistry
Hui Zhang, Cheng-Feng Xu, Chao Ren, Tian-Tian Wu, Ning Dong, Yong-Ming Yao
BACKGROUND/AIMS: Immunosuppression frequently occurs during the development of sepsis and is closely associated with poor outcome. Characteristics of immunosuppressive CD4+ T lymphocytes in sepsis have been reported to include dramatic cell loss and inactivation. p53 acts as a pivotal transcription factor in regulating cell proliferation and apoptosis, which control tumorigenesis. However, few studies have investigated the universal role of p53 in immune cells, especially in the development of sepsis...
November 19, 2018: Cellular Physiology and Biochemistry
Gang Liu, Wenxin Yan, Sujuan Ding, Hongmei Jiang, Yong Ma, Hua Wang, Jun Fang
BACKGROUND/AIMS: There are known links between inflammatory bowel disease (IBD) and changes in the microbiota of the gut and inflammation and oxidative stress. In this study, a colitis model induced by dextran sodium sulfate (DSS) in mice is used to evaluate whether the presence of bioactive peptides IRW (Ile-Arg-Trp) and IQW (Ile-Gln-Trp) peptides is advantageous. METHODS: The mice were arbitrarily assigned to the following four groups: (i) control (untreated), (ii) dextran sodium sulfate (DSS) treated, (iii) IRW-DSS treated, and (iv) IQW-DSS treated...
November 19, 2018: Cellular Physiology and Biochemistry
Letícia Batista Azevedo Rangel, Daniel de Siqueira, Olívia do Rosário Soares, Higor Scardini Santana, Emílio de Castro Miguel, Maura da Cunha, Andre Lacerda de Abreu Oliveira, Diego França Pedrosa, Ludmilla Carvalho Rangel Resgala, Helder Azevedo Rangel Neto, Neuza Felix Gomes-Rochette, Sérgio Ragi Eis, Jones Bernardes Graceli, Ian Victor Silva
BACKGROUND/AIMS: Osteoporosis is a bone metabolic disease that affects mostly post-menopausal women. There has been shown that vitamin K (VK) supplementation during menopause may decrease bone loss as well as risk of bone breaking. Aiming to clarify the beneficial role of VK in bone metabolism during menopause, we investigated mineral metabolism and bone ultrastructure of ovariectomized (OVX) mice. METHODS: To determine the effects chronic use of VK in bone structure and mineral metabolism in OVX mice, we used several methods, such as DXA, µCTScan, and SEM as well as biomolecular techniques, such as ELISA and qRT-PCR...
November 19, 2018: Cellular Physiology and Biochemistry
Siqi Tang, Bin Yao, Na Li, Shuhuang Lin, Zunnan Huang
BACKGROUND/AIMS: The neuropathies Alzheimer's disease (AD), Parkinson's disease (PD), and schizophrenia (SCZ) have different pathological mechanisms but share some common neurodegenerative features, such as gradual loss of neuronal structure and function. Dopamine beta-hydroxylase (DBH), a gene located in the chromosomal region 9q34, plays a crucial role in the process of converting dopamine into norepinephrine (NE). Several case-control studies have reported this pathway in the pathogenesis of AD, PD and SCZ...
November 19, 2018: Cellular Physiology and Biochemistry
Zhaoxia Hu, Peipei Wang, Jiaxin Lin, Xingrong Zheng, Fangji Yang, Genglin Zhang, Dabiao Chen, Junqiang Xie, Zhiliang Gao, Liang Peng, Chan Xie
BACKGROUND/AIMS: MicroRNA-197 (miR-197) has been shown to play roles in epithelialmesenchymal transition (EMT) and metastasis. The Wnt/β-catenin pathway is associated with EMT, but whether miR-197 regulatesWnt/β-catenin remains unclear. This study was to demonstrate the role of miR-197 on the Wnt/β-catenin pathway in hepatocellular carcinoma (HCC). METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-197 in 105 HCC specimens and 15 HCC cell lines...
November 19, 2018: Cellular Physiology and Biochemistry
Jian Li, Yunchao Wang, Jiayun Ge, Wenhua Li, Liangyu Yin, Zhiping Zhao, Songsong Liu, Huan Qin, Jiali Yang, Lijiang Wang, Bing Ni, Yongkang Liu, Huaizhi Wang
BACKGROUND/AIMS: Cancer stem cells (CSCs) are largely responsible for tumor relapse and metastatic behavior. Doublecortin-like kinase 1 (DCLK1) was recently reported to be a biomarker for gastrointestinal CSCs and involved in the epithelial-mesenchymal transition (EMT) and tumor progression. B cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) is a crucial regulator of CSC self-renewal, malignant transformation and EMT, and a previous study from our group showed that Bmi-1 is upregulated in pancreatic cancer progression and participates in EMT...
November 19, 2018: Cellular Physiology and Biochemistry
Xiaolan You, Yuanjie Wang, Jian Wu, Qinghong Liu, Dehu Chen, Dong Tang, Daorong Wang
BACKGROUND/AIMS: Increased expression of galectin-1 (Gal-1) in gastric cancer (GC) promotes metastasis and correlates with poor prognosis. The mechanisms by which Gal-1 promotes GC metastasis remain unknown. METHODS: Gal-1and Sphingosine-1-phosphate receptor 1 (S1PR1) were determined by immunohistochemistry(IHC) and quantitative real time polymerase chain reaction (qRT-PCR) in GC specimens. Stably transfected Gal-1 or S1PR1 into SGC7901 and MGC-803 cells, western blot and invasion assays in vitro and nude mice tumorigenicity in vivo were also employed...
November 19, 2018: Cellular Physiology and Biochemistry
Basma Sukkar, Stefan Hauser, Lisann Pelzl, Zohreh Hosseinzadeh, Itishri Sahu, Tamer Al-Maghout, Abdulla Al Mamun Bhuyan, Nefeli Zacharopoulou, Christos Stournaras, Ludger Schöls, Florian Lang
BACKGROUND/AIMS: The neurodegenerative disease Chorea-Acanthocytosis (ChAc) is caused by loss-of-function-mutations of the chorein-encoding gene VPS13A. In ChAc neurons transcript levels and protein abundance of Ca2+ release activated channel moiety (CRAC) Orai1 as well as its regulator STIM1/2 are decreased, resulting in blunted store operated Ca2+-entry (SOCE) and enhanced suicidal cell death. SOCE is up-regulated and cell death decreased by lithium. The effects of lithium are paralleled by upregulation of serum & glucocorticoid inducible kinase SGK1 and abrogated by pharmacological SGK1 inhibition...
November 19, 2018: Cellular Physiology and Biochemistry
Wen-Yueh Hung, Jer-Hwa Chang, Yu Cheng, Chi-Kuan Chen, Ji-Qing Chen, Kuo-Tai Hua, Chao-Wen Cheng, Michael Hsiao, Chi-Li Chung, Wei-Jiunn Lee, Ming-Hsien Chien
BACKGROUND/AIMS: Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI) therapy is a clinical option for non-small cell lung cancer (NSCLC) harboring activating EGFR mutations or for cancer with wild-type (WT) EGFR when chemotherapy has failed. MET receptor activation or MET gene amplification was reported to be a major mechanism of acquired resistance to EGFR-TKI therapy in NSCLC cells. Leukocyte cell-derived chemotaxin 2 (LECT2) is a multifunctional cytokine that was shown to suppress metastasis of hepatocellular carcinoma via inhibiting MET activity...
November 19, 2018: Cellular Physiology and Biochemistry
Min Luo, Yanmei Luo, Naiquan Mao, Guolin Huang, Cuifang Teng, Hanlin Wang, Junwei Wu, Xiaoli Liao, Jie Yang
BACKGROUND/AIMS: Gap junctions, which are assembled by connexins, can directly connect the cytoplasm of adjacent cells and enable gap junctional intercellular communication (GJIC) as well as metabolic coupling between neighboring cells. Here, we investigated the role of connexin 43 (Cx43) and its derived GJIC in the interplay between non-small cell lung cancer (NSCLC) cells and cancer-associated fibroblasts (CAFs). METHODS: CAFs and NSCLC cells were co-cultured with direct contact and separated using flow cytometry...
November 19, 2018: Cellular Physiology and Biochemistry
Shuang Tian, Da-Hua Liu, Dan Wang, Fu Ren, Pu Xia
BACKGROUND/AIM: Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)-based therapies have been used in many human cancers. However, some tumors are resistant to TRAIL-induced cell death. Aldehyde dehydrogenase 1 (ALDH1) is a functional marker for identification of CSCs. METHODS: In this study, we used the colony formation assay, AnnexinV/ PI double staining and PI staining to detect proliferation, apoptosis and cell cycle in ALDH1+ non-small cell lung cancer (NSCLC) cells with TRAIL treatment...
November 16, 2018: Cellular Physiology and Biochemistry
Yan-Ting Lin, Wei Liu, Yun He, Yun-Li Wu, Wan-Nan Chen, Xin-Jian Lin, Xu Lin
BACKGROUND/AIMS: Chronic hepatitis B virus (HBV) infection markedly increases the risk of development of hepatocellular carcinoma (HCC). Among the seven viral proteins that HBV encodes, HBV X protein (HBx) appears to have the most oncogenic potential. The mitochondria-associated HBx can induce oxidative stress in hepatocytes, leading to the production of abundant reactive oxygen species (ROS). High levels of ROS usually induce oxidative DNA damage and 8-hydroxy-2-deoxyguanosine (8-OHdG), also known as 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG), which is one of the major products of DNA oxidation and an important biomarker for oxidative stress and carcinogenesis...
November 16, 2018: Cellular Physiology and Biochemistry
Zhi Zuo, Jian-Xiao Shen, Yan Pan, Juan Pu, Yong-Gang Li, Xing-Hua Shao, Wan-Peng Wang
BACKGROUND/AIMS: Podocyte damage is associated with proteinuria, glomerulosclerosis and decline of renal function. This study aimed to screen critical genes associated with podocyte injury in chronic kidney disease (CKD) by weighted gene correlation network analysis (WGCNA), and explore related functions. METHODS: GSE66107, GSE93798, GSE30528, GSE32591 gene expression data including podocyte injury models or glomeruli in CKD patients were downloaded from the GEO database...
November 16, 2018: Cellular Physiology and Biochemistry
Arwa M T Al-Nahdi, Annie John, Haider Raza
BACKGROUND/AIMS: Numerous studies have reported overproduction of reactive oxygen species (ROS) and alterations in mitochondrial energy metabolism in the development of diabetes and its complications. The potential protective effects of N-acetylcysteine (NAC) in diabetes have been reported in many therapeutic studies. NAC has been shown to reduce oxidative stress and enhance redox potential in tissues protecting them against oxidative stress associated complications in diabetes. In the current study, we aimed to investigate the molecular mechanisms of the protective action of NAC on STZ-induced toxicity in insulin secreting Rin-5F pancreatic β-cells...
November 16, 2018: Cellular Physiology and Biochemistry
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