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Cellular Physiology and Biochemistry

Cheng-Ming Wei, Yi-Ji Su, Xiong Qin, Jia-Xin Ding, Qian Liu, Fang-Ming Song, Shao-Hui Zong, Jiake Xu, Bo Zhou, Jin-Min Zhao
BACKGROUND/AIMS: Extensive osteoclast formation plays a critical role in bone diseases, including rheumatoid arthritis, periodontitis and the aseptic loosening of orthopedic implants. Thus, identification of agents that can suppress osteoclast formation and bone resorption is important for the treatment of these diseases. Monocrotaline (Mon), the major bioactive component of crotalaria sessiliflora has been investigated for its anti-cancer activities. However, the effect of Mon on osteoclast formation and osteolysis is not known...
July 19, 2018: Cellular Physiology and Biochemistry
Xibo Jing, Jingxiao Yang, Lu Jiang, Jianghong Chen, Haiyan Wang
BACKGROUND/AIMS: Myocardial apoptosis plays an important role in doxorubicin (Dox) cardiotoxicity. MicroRNA-29 (miR-29) is suggested to function as an anti-fibrotic factor with potential therapeutic effects on cardiac fibrosis. However, it has not been shown whether there is an association between miR-29b and myocardial apoptosis. METHODS: Male Wistar rats were transfected with miR-29b agomir by local delivery to the myocardium prior to Dox treatment. Rat cardiomyocytes were pretreated with miR-29b mimics or inhibitor followed by Dox incubation in vitro...
July 19, 2018: Cellular Physiology and Biochemistry
Juan Jin, Kang Hu, Meiyu Ye, Diandian Wu, Qiang He
BACKGROUND/AIMS: The purpose of this study was to investigate the impact of rapamycin (RAP) on autophagy in podocytes and the therapeutic effects of RAP on idiopathic membranous nephropathy (IMN). METHODS: We established an in vitro model of IMN by preconditioning mouse podocytes with puromycin aminonucleoside (PAN). A Cell Counting Kit-8 was used to detect the proliferation of each group of podocytes. Podocyte apoptosis was analyzed by flow cytometry via annexin V/propidium iodide dual staining...
July 19, 2018: Cellular Physiology and Biochemistry
Sha She, Lingyu Jiang, Zhenfang Zhang, Min Yang, Huaidong Hu, Peng Hu, Yong Liao, Yixuan Yang, Hong Ren
BACKGROUND/AIMS: C reactive protein (CRP) levels are elevated in many diseases, including malignant tumors and cardiovascular disorders. In this study, the protein interaction network for CRP was evaluated to determine the importance of CRP and its interacting proteins in the molecular pathogenesis of hepatocellular carcinoma (HCC). METHODS: Isobaric tags for relative and absolute quantitation (iTRAQ) and mass spectrometry were used to identify CRP interacting proteins in SMMC7721 cells...
July 19, 2018: Cellular Physiology and Biochemistry
Jing Xu, Lin-Jiang Chen, Jian Yu, Han-Jing Wang, Fan Zhang, Qiong Liu, Jing Wu
Diabetic retinopathy (DR) is a common and devastating microvascular complication of diabetes and a major cause of acquired blindness in young adults. Advanced glycation end products (AGEs) accumulated under hyperglycemic conditions are thought to play an important role in the pathogenesis of DR. AGEs can exert their deleterious effects by acting directly to induce aberrant crosslinking of extracellular matrix proteins, to increase vascular stiffness, altering vascular structure and function. Moreover, AGEs binding to the receptor for AGEs (RAGE) evokes intensive intracellular signaling cascades that leading to endothelial dysfunction, elaboration of key proinflammatory cytokines and proangiogenic factors, mediating pericyte apoptosis, vascular inflammation and angiogenesis, as well as breakdown of the inner blood-retinal barrier (BRB), the end result of all these events is damage to the neural and vascular components of the retina...
July 19, 2018: Cellular Physiology and Biochemistry
Zhong-Ling Qiu, Wei-Jun Wei, Zhen-Kui Sun, Chen-Tian Shen, Hong-Jun Song, Xin-Yun Zhang, Guo-Qiang Zhang, Xiao-Yue Chen, Quan-Yong Luo
BACKGROUND/AIMS: As biomarkers, circulating tumor cells (CTCs) from solid tumors can predict metastases and prognoses, and help monitor treatment efficacy. However, conventional CellSearch methods have low sensitivity to differentiated thyroid cancer (DTC) CTCs. In this study, for the first time, we used negative enriching (NE) immunofluorescence-in situ hybridization (iFISH) of chromosome 8 to capture and identify CTCs in DTC patients; and investigated how CTCs correlate with clinicopathological factors and prognosis in DTC patients with distant metastases (DM)...
July 19, 2018: Cellular Physiology and Biochemistry
Man Li, Zhen-Hua Zhou, Ting Bao, Xin Zhang, Xiao-Jun Zhu, Shu-Gen Jin, Ya-Ting Gao, Xue-Hua Sun, Yue-Qiu Gao
BACKGROUND/AIMS: To investigate the clinical effects of the combination therapy with Bushen Formula (BSF) plus enticavir (ETV) on chronic hepatitis B (CHB) patients with suboptimal response to ETV and explore the regulatory mechanisms of BSF on B cells-mediated humoral immunity. METHODS: Sixty-four HBeAg-positive CHB patients with suboptimal response to ETV were enrolled, and were randomly assigned into control group (C-Group, placebo combined with ETV for 12 months) or treatment group (T-Group, BSF combined with ETV for 12 months)...
July 19, 2018: Cellular Physiology and Biochemistry
Fei Zhang, Wu-Xun Peng, Lei Wang, Jian Zhang, Wen-Tao Dong, Jian-Hua Wu, Huai Zhang, Jian-Bo Wang, Yin Zhao
BACKGROUND/AIMS: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. METHODS: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone...
July 19, 2018: Cellular Physiology and Biochemistry
Jiqian Zhang, Wei Dai, Pengcheng Geng, Li Zhang, Qilian Tan, Dan Cheng, Pengfei Wei, Zhilai Yang, Lei Zhang, Erwei Gu, Guanghong Xu, Chaozhao Liang, Xuesheng Liu
BACKGROUND/AIMS: Autophagy is a well-known pathway to "clean" the misfolded mutant huntingtin protein (mHtt), which plays a considerable role in polyglutamine diseases. To date, there have been few studies of the choice of anesthetic during surgery in patients with polyglutamine diseases and evaluation of the effects and underlying mechanisms of anesthetics in these patients. METHODS: GFP-Htt (Q74)-PC12 cells, which stably express green fluorescent protein-tagged Htt protein containing 74 glutamine repeating units, were used throughout this study...
July 19, 2018: Cellular Physiology and Biochemistry
Xiaohui Ji, Xiaoyun Yang, Nan Wang, Meiyun Kang, Yaping Wang, Liucheng Rong, Yongjun Fang, Yao Xue
BACKGROUND/AIMS: Y+LAT1 protein, encoded by the SLC7A7 gene (a member of the SLC7 family), forms the cationic amino acid transport system y+L (system y+L). This system transports cationic amino acids such as arginine and lysine out of the cell. Arginine, in particular, is critical for T-cell activation and function in the immune response. METHODS: We analyzed the role of the SLC7A7 gene in the cellular activities of Jurkat cells, specifically the cell cycle and cell proliferation, apoptosis, migration, and invasion...
July 19, 2018: Cellular Physiology and Biochemistry
Guangming Wang, Fangce Wang, Jie Ren, Yue Qiu, Wenjun Zhang, Shane Gao, Danjing Yang, Zhigang Wang, Aibin Liang, Zhengliang Gao, Jun Xu
BACKGROUND/AIMS: Alternative splicing and DNA damage exhibit cross-regulation, with not only DNA damage inducing changes in alternative splicing, but alternative splicing itself possibly modulating the DNA damage response (DDR). Sirt1, a prominent anti-aging player, plays pivotal roles in the DDR. However, few studies have examined alternative splicing with DNA damage in neural stem cells (NSCs) and, in essence, nothing is known about whether SIRT1 regulates alternative splicing. Hence, we investigated the potential involvement of Sirt1-mediated alternative splicing in the NSC DDR...
July 19, 2018: Cellular Physiology and Biochemistry
Juan Jin, Li Zhao, Wenli Zou, Wei Shen, Hongjuan Zhang, Qiang He
BACKGROUND/AIMS: Autophagy plays an essential role in lupus nephritis (LN)-induced kidney injury, although the mechanism of action remains obscure. We investigated the role of cyclooxygenase-2 (COX-2) and the ATF4 endoplasmic reticulum (ER) stress pathway in LN-induced podocyte autophagy. METHODS: We evaluated podocyte autophagy in a mouse model of LN. Protein levels of COX-2 and ATF4, and markers of autophagy, were evaluated by immunofluorescence and western blotting...
July 19, 2018: Cellular Physiology and Biochemistry
Xiafei Fu, Yuanli He, Xuefeng Wang, Dongxian Peng, Xiaoying Chen, Xinran Li, Qing Wan
BACKGROUND/AIMS: Several miRNAs have been reported to be involved in the pathogenesis of polycystic ovarian syndrome (PCOS). However, the biological roles of miR-16 and its molecular mechanisms in PCOS development remain to be elucidated. METHODS: qRT-PCR was performed to detect the expression levels of miR-16 and programmed cell death protein 4 (PDCD4). GCs proliferation, cell cycle distribution and apoptosis were examined by MTT assay and flow cytometry analysis...
July 19, 2018: Cellular Physiology and Biochemistry
Hanzi Xu, Zhen Gong, Siying Zhou, Sujin Yang, Dandan Wang, Xiu Chen, Jiaqi Wu, Liucheng Liu, Shanliang Zhong, Jianhua Zhao, Jinhai Tang
BACKGROUND/AIMS: Emerging evidence suggests that curcumin possesses chemopreventive properties against various cancers. However, its poor bioavailability limits its clinical application. In this study, we aimed to utilize encapsulation in liposomes (Lipo) as a strategy for the clinical administration of curcumin for endometrial carcinoma (EC). METHODS: Curcumin was encapsulated in a liposomal delivery system to prepare a formulation of liposomal curcumin (LC). EC cell lines Ishikawa and HEC-1 were treated with the compound and cell proliferation was measured using MTT assay...
July 18, 2018: Cellular Physiology and Biochemistry
Lianjie Hou, Meiying Xie, Lingbo Cao, Jia Shi, Guli Xu, Chingyuan Hu, Chong Wang
BACKGROUND/AIMS: Brown adipose tissue (BAT) is critical for mammals' survival in the cold environment. BAT-dependent non-shivering thermogenesis is attributed to uncoupling protein 1 (UCP1)'s disengagement of oxidative phosphorylation from ATP synthesis and dissipates energy as heat. Thus individuals with a substantial amount of BAT are better equipped during cold stress and less likely to become obese. Recently, our laboratory has shown pig adipocytes have no UCP1 protein. The inability of newborn piglets to generate heat contributed to its high death rate...
July 18, 2018: Cellular Physiology and Biochemistry
Issa Olakunle Yusuf, Pei-Hsun Cheng, Hsiu-Mei Chen, Yu-Fan Chang, Chih-Yi Chang, Han-In Yang, Chia-Wei Lin, Shaw-Jenq Tsai, Jih-Ing Chuang, Chia-Ching Wu, Bu-Miin Huang, H Sunny Sun, Shang-Hsun Yang
BACKGROUND/AIMS: Huntington's disease (HD) is a heritable neurodegenerative disorder, and there is no cure for HD to date. A type of fibroblast growth factor (FGF), FGF9, has been reported to play prosurvival roles in other neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. However, the effects of FGF9 on HD is still unknown. With many similarities in the cellular and pathological mechanisms that eventually cause cell death in neurodegenerative diseases, we hypothesize that FGF9 might provide neuroprotective functions in HD...
July 18, 2018: Cellular Physiology and Biochemistry
Tengda Li, Mingli Gu, Peng Liu, Yun Liu, Jie Guo, Weiwei Zhang, Anmei Deng, Cheng Qian
BACKGROUND/AIMS: Long noncoding RNAs (lncRNAs) are important regulators of biological processes and they contribute to the pathological developments of various diseases, including autoimmune diseases. To gain the further understanding, we estimate the expression of lncRNAs in primary immune thrombocytopenia (ITP). METHODS: In this study, microarray studies were performed to characterize expression profiles of various lncRNAs and mRNAs in blood samples collected from ITP patients...
July 18, 2018: Cellular Physiology and Biochemistry
Jiahui Chu, Yongfei Li, Xuemei Fan, Jingjing Ma, Jun Li, Guangping Lu, Yanhong Zhang, Yi Huang, Wei Li, Xiang Huang, Ziyi Fu, Yongmei Yin, Hongyan Yuan
BACKGROUND/AIMS: High levels of cancer stem cells (CSCs) in patients with triple-negative breast cancer (TNBC) correlate with risk of poor clinical outcome and possibly contribute to chemoresistance and metastasis in patients with highly malignant TNBC. Aberrant microRNA expression is associated with the dysfunction of self-renewal and proliferation in cancer stem cells, while there is little information about the TNBC-specific microRNAs in regulating CSC ability. METHODS: Solexa deep sequencing was performed to detect the expression levels of TNBC or non-TNBC stem cells (CSCs) microRNAs...
July 18, 2018: Cellular Physiology and Biochemistry
Jianjun Xu, Cai Lin, Tingting Wang, Peng Zhang, Zhengjun Liu, Caijiao Lu
BACKGROUND/AIMS: Ergosterol (ER) is the primary sterol found in fungi and is named after the ergot fungus. A variety of pharmacological activities have been reported for ER, including antioxidative, anti-proliferative, and anti-inflammatory effects, although its role in sepsis remains unclear. METHODS: The protective effect of ER on lipopolysaccharide (LPS)-induced sepsis myocardial injury was evaluated both in vivo and in vitro. Rats were pretreated with ER and then with LPS...
July 18, 2018: Cellular Physiology and Biochemistry
Hongxue Sun, Di Zhong, Cheng Wang, Yilei Sun, Jiaying Zhao, Guozhong Li
BACKGROUND/AIMS: This study investigated the role of the microRNA miR-298 and its target Act1 in ischemic stroke. METHODS: Cell viability was assessed with the 3-(4,5-dimethythiazol-2- yl)-2,5-diphenyl tetrazolium bromide assay. Apoptotic cells were detected by flow cytometry, and mRNA and protein expression were assessed by quantitative real-time PCR and western blotting, respectively. The regulatory relationship between miR-298 and Act1 was evaluated with the luciferase assay...
July 18, 2018: Cellular Physiology and Biochemistry
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