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Molecular Plant-microbe Interactions: MPMI

Cleverson Carlos Matiolli, Maeli Melotto
Yeast-two-hybrid (Y2H) cDNA library screening is a valuable tool to uncover protein-protein interactions and represents a widely used method to investigate protein function. However, cDNA libraries with low transcript representation limits the depth of the screening. We have developed a Y2H library with cDNA made from Arabidopsis leaves exposed to several stressors as well as untreated leaves. The library was built using pooled mRNA extracted from plants challenged with plant and human bacterial pathogens, the flg22 elicitor, the phytotoxin coronatine, and several hormones associated with environmental stress responses...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
Amjad M Husaini, Aafreen Sakina, Souliha R Cambay
Fusarium oxysporum, a ubiquitous soil-borne pathogen causes devastating vascular wilt in more than 100 plant species and ranks fifth among top ten fungal plant pathogens. It has emerged as a human pathogen too, causing infections in immune-compromised patients. It is, therefore, important to gain insight into the molecular processes involved in the pathogenesis of this trans-kingdom pathogen. A complex network comprising of interconnected and over lapping signal pathways; mitogen-activated protein kinase (MAPK) signaling pathways, Ras proteins, G-protein signaling components and their downstream pathways, components of the velvet (LaeA/VeA/VelB) complex and cAMP pathways, is involved in perceiving the host...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
Nadia Lombardi, Stefania Vitale, David Turrà, Massimo Reverberi, Corrado Fanelli, Francesco Vinale, Roberta Marra, Michelina Ruocco, Alberto Pascale, Giada d'Errico, Sheridan Lois Woo, Matteo Lorito
Plant roots release complex mixtures of bioactive molecules including compounds that affect the activity and modify the composition of the rhizosphere microbiome. In this work, we investigated the initial phase of the interaction between tomato and an effective biocontrol strain of Trichoderma harzianum (T22). We found that root exudates (RE), obtained from plants grown in a split root system and exposed to a choice of biotic and abiotic stress factors (wounding, salt, pathogen attack), stimulate the growth and act as chemoattractants of the biocontrol fungus...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
Guirong Tang, Qiong Li, Shenghui Xing, Ningning Li, Zheng Tang, Liangliang Yu, Junhui Yan, Xuan Li, Li Luo
Agrobacterium tumefaciens infects and causes crown galls in dicot plants by transferring T-DNA from the Ti plasmid to the host plant via a type IV secretion system (T4SS). This process requires appropriate environmental conditions, certain plant secretions and bacterial regulators. In our previous work, a member of the LysR family of transcriptional regulators (LsrB) in Sinorhizobium meliloti was found to modulate its symbiotic interactions with the host plant alfalfa. However, the function of its homologue in A...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
Vittorio Filippo Nicolis, Eduard Venter
Plants respond in a similar manner to aphid feeding as to pathogen attack. Diuraphis noxia is a specialist aphid, feeding only on selected grasses that include wheat, barley, and oats. The wheat-Diuraphis noxia interaction is characterized by very similar responses as seen in wheat-pathogen interactions with none of the underlying resistance pathways and genes characterized yet. From wheat harboring the Dn1 resistance gene, we have identified a nucleotide-binding leucine-rich repeat (NLR) gene containing two integrated domains (IDs)...
March 13, 2018: Molecular Plant-microbe Interactions: MPMI
Chunxia Li, Shiyao Liu, Peiyi Liu, Yaxin Wang, Chunli Xu, Jun Tao, Chaozu He
Riboswitches are RNA elements that sense metabolites and control gene expression. Recently, the yybP-ykoY riboswitches were found to sense manganese (Mn2+) and regulate the expression of diverse genes. Here, we show that the leader RNA (a yybP-ykoY RNA) of yebN in Xanthomonas oryzae pv. oryzae (Xoo) also functions as a sensor of Mn2+. This leader RNA detects Mn2+ levels in plants and is essential to Xoo virulence. Our data also indicate that Mn2+ is not only required as a microelement for plant growth but also acts as a defense molecule to inhibit pathogen growth...
March 9, 2018: Molecular Plant-microbe Interactions: MPMI
Hsiao-Han Lin, Hsin-Mei Huang, Manda Yu, Erh-Min Lai, Hsiao-Lin Chien, Chi-Te Liu
The bacterial type VI secretion system (T6SS) has been considered the armed force of bacteria because it can deliver toxin effectors to prokaryotic or eukaryotic cells for survival and fitness. Although many legume symbiotic rhizobacteria encode T6SS in their genome, the biological function of T6SS in these bacteria is still unclear. To elucidate this issue, we used Azorhizobium caulinodans ORS571 and its symbiotic host Sesbania rostrata as our research model. By using T6SS gene deletion mutants, we found that T6SS provides A...
March 8, 2018: Molecular Plant-microbe Interactions: MPMI
Jesús Martínez-Cruz, Diego F Romero, Fernando de la Torre, Dolores Fernández-Ortuño, Juan Antonio Torés, Antonio de Vicente, Alejandro Perez-Garcia
Podosphaera xanthii is the main causal agent of powdery mildew disease in cucurbits. In a previous study, we determined that P. xanthii expresses approximately 50 Podosphaera effector candidates (PECs), identified based on the presence of a predicted signal peptide and the absence of functional annotation. In this work, we used host-induced gene silencing (HIGS), employing Agrobacterium tumefaciens as a vector for the delivery of the silencing constructs (ATM-HIGS), to identify genes involved in early plant-pathogen interaction...
March 7, 2018: Molecular Plant-microbe Interactions: MPMI
Nathalie Marquez, Maria Lorena Giachero, Adrien Gallou, Humberto Debat, Sylvie Canenbrouck, Julio Di Rienzo, Maria Pozo, Daniel A Ducasse, Stéphane Declerck
Macrophomina phaseolina is a soil borne fungal pathogen with a wide host range that causes charcoal rot in soybean (Glycine max (L.) Merr). Control of the disease is a challenge, due to the absence of genetic resistance and effective chemical control. Alternative or complementary measures are needed, such as the use of biological control agents, in an integrated approach. Several studies have demonstrated the role of arbuscular mycorrhizal fungi (AMF) in enhancing plant resistance or tolerance to biotic stresses, decreasing the symptoms and pressure caused by various pests and diseases, among which M...
March 2, 2018: Molecular Plant-microbe Interactions: MPMI
Vera Safronova, Andrey Belimov, Anna Sazanova, Elizaveta Chirak, Alla Verkhozina, Irina Kuznetsova, Evgeny Andronov, Jan Puhalsky, Igor Tikhonovich
Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification of the isolates sequencing of rrs, ITS (internal transcribed spacer) region and housekeeping genes recA, glnII and rpoB was used. Nine fast-growing isolates were Mesorhizobium-related: eight strains were identified as M. japonicum and one isolate belonged to M. kowhaii. The only slow-growing isolate was identified as Bradyrhizobium sp. Two strains, M. japonicum Opo-242 and Bradyrhizobium sp...
March 2, 2018: Molecular Plant-microbe Interactions: MPMI
Zhiwei Huang, Qingxuan Zhou, Pan Sun, Jing Yang, Minliang Guo
Agrobacterium tumefaciens is the agent that causes crown gall tumor disease on more than 140 species of dicotyledonous plants. Chemotaxis of A. tumefaciens toward the wound sites of the host plant is the first step to recognize the host. CheW is a coupling protein that bridges the histidine kinase CheA and the chemoreceptors to form the chemotaxis core signaling complex and plays a crucial role in the assembly and function of the large chemosensory array. Unlike all previously reported chemotaxis systems, A...
March 2, 2018: Molecular Plant-microbe Interactions: MPMI
G Adam Mott, Darrell Desveaux, David S Guttman
The first step in the plant immune response to pathogen challenge involves the perception of conserved epitopes, called microbe-associated molecular patterns (MAMPs), by cell-surface pattern recognition receptors (PRRs). Given the key roles that MAMPs and PRRs play in plant innate immunity, great effort has been expended to identify these molecules. Current methods for assaying these immune responses are often limited in their resolution and throughput, and consequently, there is a need for medium- to high-throughput methodologies...
February 28, 2018: Molecular Plant-microbe Interactions: MPMI
Kaixuan Duan, Christopher J Willig, Joann R De Tar, William G Spollen, Zhanyuan J Zhang
Agrobacterium tumefaciens is a plant pathogen that causes crown gall disease. This pathogen is capable of transferring the T-DNA from its Ti plasmid to the host cell and, then, integrating it into the host genome. To date, this genetic transformation ability has been harnessed as the dominant technology to produce genetically modified plants for both basic research and crop biotechnological applications. However, little is known about the interaction between Agrobacterium tumefaciens and host plants, especially the host responses to Agrobacterium infection and its associated factors...
February 28, 2018: Molecular Plant-microbe Interactions: MPMI
Shawna L Rowe, Jeffrey S Norman, Maren Friesen
Plants and microbes are dependent on chemical signals as a means of inter-kingdom communication. There are two predicted paths for the evolution of these signals. Ritualization, the evolution of signals from cues, is the oft-assumed pathway for the evolution of plant-microbe communication systems. However, plant-microbe signaling may evolve from coercive interactions as well, a process known as sensory manipulation. Here, we aim to highlight the prevalence of coercive interactions and discuss sensory manipulation in the context of plant-microbe interactions...
February 23, 2018: Molecular Plant-microbe Interactions: MPMI
Yixin Ge, Jae Hoon Lee, Baishi Hu, Youfu Frank Zhao
Extensive use of antibiotic streptomycin to control fire blight disease of apples and pears, caused by the enterobacterial plant pathogen Erwinia amylovora, leads to the development of streptomycin-resistant strains in the United States and elsewhere. Kasugamycin (Ksg, kasuminR) has been permitted to be used as an alternative or replacement to control this serious bacterial disease. In this study, we investigated the role of two major peptide ABC-transporter systems in E. amylovora, the dipeptide permease (Dpp) and oligopeptide permease (Opp), in conferring sensitivity to Ksg and blasticidin S (BcS)...
February 23, 2018: Molecular Plant-microbe Interactions: MPMI
Thibault Meyer, Sébastien Renoud, Armelle Vigouroux, Aurélie Miomandre, Vincent Gaillard, Isabelle Kerzaon, Claire Prigent-Combaret, Gilles Comte, Solange Morera, Ludovic Vial, Lavire Céline
Regulatory factors are key components for the transition between different lifestyles to ensure rapid and appropriate gene expression upon perceiving environmental cues. Agrobacterium fabrum C58 (formerly called A. tumefaciens C58) has two contrasting lifestyles: it can interact with plants either as a rhizosphere inhabitant (rhizospheric lifestyle), or as a pathogen that creates its own ecological niche in a plant tumor via its tumor-inducing plasmid (pathogenic lifestyle). Hydroxycinnamic acids are known to play an important role in the pathogenic lifestyle of Agrobacterium but can be degraded in A...
February 20, 2018: Molecular Plant-microbe Interactions: MPMI
Amelia H Lovelace, Amy Smith, Brian H Kvitko
Pattern triggered Immunity (PTI) can confer broad defense against diverse microbes and pathogens with disparate lifestyles through the detection of microbial extracellular signatures by surface exposed pattern recognition receptors. However, unlike recognition of pathogen effectors by cytosolic resistance proteins, PTI is typically not associated with a host cell programmed cell death response. Although host PTI signaling has been extensively studied, the mechanisms by which it restricts microbial colonization is poorly understood...
February 20, 2018: Molecular Plant-microbe Interactions: MPMI
Richard Paul Jacoby, Anna Martyn, Stan Kopriva
The ability of microorganisms to use root-derived metabolites as growth substrates is a key trait for success in the rhizospheric niche. However, few studies describe which specific metabolites are consumed, or to what degree microbial strains differ in their substrate consumption patterns. Here we present an LC-MS exometabolomic study of three bacterial strains cultivated using either glucose or Arabidopsis thaliana root extract as the sole carbon source. Two of the strains were previously isolated from field-grown Arabidopsis roots, the other is Escherichia coli included as a comparison...
February 19, 2018: Molecular Plant-microbe Interactions: MPMI
Emmanouil Domazakis, Doret Wouters, Richard Visser, Sophien Kamoun, Matthieu Haj Joosten, Vivianne G A A Vleeshouwers
The ELICITIN RESPONSE (ELR) protein from Solanum microdontum can recognize INF1 elicitin of Phytophthora infestans and trigger defense responses. ELR is a receptor-like protein (RLP) that lacks a cytoplasmic signaling domain and is anticipated to require interaction with a signaling-competent receptor-like kinase (RLK). SUPPRESSOR OF BIR1-1 (SOBIR1) has been proposed as a general interactor for RLPs involved in immunity and as such, is a potential interactor for ELR. Here we investigate whether SOBIR1 is required for response to INF1 and resistance to P...
February 16, 2018: Molecular Plant-microbe Interactions: MPMI
Javier F Tabima, Michael D Coffey, Inga Zasada, Niklaus J Grünwald
Population genetics is a powerful tool to understand patterns and evolutionary processes that are involved in plant pathogen emergence and adaptation to agricultural ecosystems. We are interested in studying the population dynamics of Phytophthora rubi, the causal agent of Phytophthora root rot in raspberry. P. rubi is found in the western U.S., where the majority of the fresh and processed raspberries are produced. We used genotyping-by-sequencing to characterize genetic diversity in populations of P. rubi sampled in the U...
February 16, 2018: Molecular Plant-microbe Interactions: MPMI
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