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Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission

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https://www.readbyqxmd.com/read/29790791/age-associated-decrease-of-sialin-in-salivary-glands
#1
L Li, H Wang, L Hu, X Wu, B Zhao, Z Fan, C Zhang, J Wang, S Wang
Aging salivary glands are characterized by a reduced volume of acini, increased duct volume, decline in the rate of synthesis of proteins and their mRNA, and decreased saliva flow. Sialin is a versatile anion transporter that is highly expressed in salivary glands and may also participate in maintenance of salivary gland function. We investigated age-associated sialin expression in salivary glands. Submandibular glands of mice and human parotid gland were collected at different ages. Western blot and real-time reverse transcription-polymerase chain reaction were used to analyze the protein and mRNA expression levels of sialin, respectively...
May 23, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29790789/assessment-of-the-association-between-the-frequency-of-micronucleus-and-p-16ink4a-ki-67-co-expression-in-patients-with-cervical-intraepithelial-lesions
#2
G Gashi, V Mahovlić, E Bahtiri, F Kurshumliu, A Podrimaj-Bytyqi, I R Elezaj
Human papilloma virus (HPV) infection is the main etiological factor for cervical intraepithelial lesions (CIN). An important characteristic of this process is the loss of genome stability. Therefore, it is imperative to use biomarkers of DNA damage caused by genomic instability to identify high risk individuals. We investigated the frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) of 20 patients, diagnosed as histologically CIN 1 and 10 healthy controls. We also examined the frequency of other nuclear anomalies including nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL of patients with CIN 1 and healthy controls, and evaluated the benefits of p16INK4a and Ki-67 (p16INK4a /Ki-67) immunohistochemical double staining for identifying cervical squamous cells that express HPV E6/E7 oncogenes...
May 23, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29781718/comparsion-of-staining-characteristics-of-toto-bodies
#3
S S Padala, M A Kiresur, A Ananthaneni, V S Guduru, H K Puneeth, B Bhavana
Toto bodies are eosinophilic structures that resemble the cells of the superficial cell layer of the oral epithelium. Toto bodies commonly are associated with inflammatory gingival and other mucosal lesions including pyogenic granuloma, irritational fibroma, epulis fissuratum, peripheral giant cell granuloma and inflammatory hyperplastic gingivitis. We evaluated staining characteristics of Toto bodies to establish their origin and to identify their significance in lesions. We investigated pyogenic granuloma, fibroma and leukoplakia with epithelium that exhibited Toto bodies after hematoxylin and eosin (staining...
May 21, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29701117/the-histological-structure-and-histochemistry-of-the-mucosa-of-the-nasal-conchae-in-geese-anser-anser
#4
İ S Harem, M Kocak, E Karadag Sari
We investigated the histological structure and histochemistry of the nasal conchae of geese and compared these structures with those of other avian species. The rostral, middle and caudal conchae were dissected from the nasal cavity of eight geese, fixed in Carnoy's solution and embedded in paraffin. The entrance of the rostral concha was lined by keratinized stratified squamous epithelium, which toward the middle concha was replaced by modified keratinized squamous epithelium, the deep layer of which opened into tubular glandular structures containing secretory epithelium on crypt-like invaginations...
April 27, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29701111/detection-of-cytokinins-and-auxin-in-plant-tissues-using-histochemistry-and-immunocytochemistry
#5
C S Bedetti, N C Jorge, Fcg Trigueiro, G P Bragança, L V Modolo, Rms Isaias
We report a new method for histochemical localization of cytokinins (CKs) in plant tissues based on bromophenol blue/silver nitrate staining. The method was validated by immunohistochemistry using anti-trans-zeatin riboside antibody. Indole-3-acetic acid (auxin, IAA) was localized by anti-IAA antibody in plant tissues as a proof for IAA histolocalization. We used root sections, because they are major sites of CKs synthesis, and insect galls of Piptadenia gonoacantha that accumulate IAA. Immunostaining confirmed the presence of zeatin and sites of accumulation of IAA indicated by histochemistry...
April 27, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29701106/thymoquinone-is-protective-against-2-3-7-8-tetrachlorodibenzo-p-dioxin-induced-hepatotoxicity
#6
M E Erdemli, B Yigitcan, M Gul, H G Bag, S Gul, Z Aksungur
We investigated changes in rat liver tissues following administration of thymoquinone (TQ) against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced hepatotoxicity. Fifty rats were assigned randomly to five groups of 10 as follows: control, corn oil, TCDD, TQ and TCDD + TQ. Biochemical and histopathological analyses were conducted on liver tissue. We found that 30 day TCDD administration caused histopathological changes in liver including thickening of Glisson's capsule, intracytoplasmic vacuolization in hepatocytes, sinusoidal dilation, vascular and sinusoidal congestion and inflammatory cell infiltration...
April 27, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29701082/the-protective-effect-of-melatonin-in-lungs-of-newborn-rats-exposed-to-maternal-nicotine
#7
A Yildiz, N Vardi, M G Karaaslan, B Ates, E Taslidere, M Esrefoglu
We investigated possible healing effects of melatonin (MEL) on biochemical and histological changes in the lungs of rat offspring caused by exposure to nicotine (NT) in utero. Pregnant rats were divided randomly into five groups. The SP group was treated with physiological saline. The EA group was treated with ethyl alcohol. The MEL group was treated with MEL. The NT group was treated with NT. The NT + MEL group was treated with NT and MEL. At the end of the study, the biochemistry and histopathology of lung tissue of the offspring were examined...
April 27, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29701069/immunohistochemical-examination-of-osteopontin-and-sirtuin-1-expression-in-cattle-tuberculosis
#8
M Sevtekin, O Ozmen
We evaluated osteopontin (OPN) and sirtuin-1 (SIRT-1) expression in bovine tuberculosis lesions. The organs of cattle with tuberculosis (TB) were examined for morphology, histopathology and immunohistochemistry of OPN and SIRT-1 expression. Macroscopic lesions commonly were localized in the lungs and mediastinal lymph nodes as well as in livers and hearts. Mycobacterial agents were detected in lesions using the Ziehl-Neelsen method. No OPN or SIRT-1 expression was detected by immunohistochemistry in normal tissues, whereas a marked increase in their expressions was observed in tuberculous lesions...
April 27, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29693452/the-effects-of-aflatoxin-b-1-on-growth-hormone-regulated-gene-1-and-interaction-between-dna-and-aflatoxin-b-1-in-broiler-chickens-during-hatching
#9
E Gülbahçe Mutlu, E Arslan, Y Öznurlu, H Özparlak
Many types of aflatoxin cause problems for both public and animal health. Aflatoxin B1 (AFB1 ) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB1 decreases feed quality, egg production and fertility of hatching eggs. Also, AFB1 alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB1 on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation...
April 25, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29671622/changes-in-kidney-tissue-and-effects-of-erythropoietin-after-acute-heart-failure
#10
A Güven Bağla, M Içkin Gülen, F Ercan, F Aşgün, E Ercan, C Bakar
Impairment of cardiac function causes renal damage. Renal failure after heart failure is attributed to hemodynamic derangement including reduced renal perfusion and increased venous pressure. One mechanism involves apoptosis and is defined as cardiorenal syndrome type 1. Erythropoietin (EPO) is a cytokine that induces erythropoiesis under hypoxic conditions. Hypoxia inducible factor 1 alpha (HIF-1α) plays a regulatory role in cellular response to hypoxia. Protective effects of EPO on heart, kidney and nervous system are unrelated to red blood cell production...
April 19, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29652183/effects-of-sunitinib-on-immunoreactivity-of-vimentin-e-cadherin-and-s100-in-kidneys-of-streptozotocin-induced-diabetic-mice
#11
S O Akarca-Dizakar, H Aktuğ, F Oltulu, G Öktem, A Yavaşoğlu, E Açikgöz, G Yiğittürk, K Demir, A Uysal
Diabetes mellitus (DM) affects many organs including kidney. Tyrosine kinase can cause hypoglycemia and sunitinib is an inhibitor of tyrosine kinase. We investigated the possible effects of sunitinib on the kidney of streptozotocin (STZ) induced type 1 diabetic mice. We used 28 CD 1 type male mice divided into four groups of seven. Type 1 diabetes was induced by injection of STZ. Group 1 was the untreated control. Group 2 comprised non-diabetic mice + sunitinib. Both groups 1 and 2 exhibited normal blood glucose levels...
April 13, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29644878/investigation-of-the-protective-effects-of-crocin-on-acrylamide-induced-small-and-large-intestine-damage-in-rats
#12
S Gedik, M E Erdemli, M Gul, B Yigitcan, H Gozukara Bag, Z Aksungur, E Altinoz
We investigated repair of acrylamide (AA) induced damage in intestines by administration of crocin. We used 40 male Wistar rats in four groups of 10 animals: control, AA, crocin, and AA + crocin groups. We investigated biochemical and histological changes to small and large intestine. AA ingestion decreased glutathione (GSH) levels and total antioxidant status (TAS) in the intestine compared to the control group, while superoxide dismutase (SOD) and catalase (CAT) activities, and total oxidant status (TOS) and malondialdehyde (MDA) levels were increased...
April 12, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29642726/donkey-milk-kefir-induces-apoptosis-and-suppresses-proliferation-of-ehrlich-ascites-carcinoma-by-decreasing-inos-in-mice
#13
Obb Esener, B M Balkan, E I Armutak, A Uvez, G Yildiz, M Hafizoglu, N Yilmazer, E Gurel-Gurevin
Donkey milk and donkey milk kefir exhibit antiproliferative, antimutagenic and antibacterial effects. We investigated the effects of donkey milk and donkey milk kefir on oxidative stress, apoptosis and proliferation in Ehrlich ascites carcinoma (EAC) in mice. Thirty-four adult male Swiss albino mice were divided into four groups as follows: group 1, administered 0.5 ml water; group 2, administered 0.5 ml water + EAC cells; group 3, administered 0.5 ml donkey milk + EAC cells; group 4, administered 0.5 ml donkey milk kefir + EAC cells...
April 12, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29611725/isoflurane-post-conditioning-influences-myocardial-infarct-healing-in-rats
#14
I Agnic, N Filipovic, K Vukojevic, M Saraga-Babic, I Grkovic
Isoflurane post-conditioning causes an early increase in cardiac progenitor cells; however, during the chronic phase of infarct healing, the number was smaller compared to control, which suggests a positive effect on infarct scar maturity. Myofibroblasts participate in early phase infarct contraction, but their number is small in a mature scar. We investigated whether isoflurane post-conditioning stimulates differentiation of progenitor cells to myofibroblasts and to verify our hypothesis that isoflurane post-conditioning improves maturation of a myocardial scar...
April 3, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29611720/staining-for-viability-testing-germination-and-maturation-of-sambucus-nigra-l-pollen-in-vitro
#15
J Kosel, L Vižintin, A Majer, B Bohanec
Freshly released pollen of black elderberry (Sambucus nigra L.) was incubated under various culture conditions until germination was achieved. Optimal conditions for germination were determined and used for maturation of unicellular microspores in vitro. Staining with 5-diphenyltetrazolium bromide, propidium iodide and iodine potassium iodide was used to assess pollen viability, nuclear phase and maturation, respectively. The germination rate was highest when fresh pollen was agitated at 40 rpm in Petri dishes containing a liquid medium consisting of Brewbaker and Kwack salts, 15% (w/v) sucrose, 500 mg/l MES sodium salt, at pH 5...
April 3, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29611713/effects-of-thymoquinone-on-liver-mirnas-and-oxidative-stress-in-ehrlich-acid-mouse-solid-tumor-model
#16
I Meral, M Pala, F Akbas, S Ustunova, C Yildiz, M H Demirel
We investigated the effects of thymoquinone (TQ) on the expression of liver microRNAs (miRNAs), liver histopathology and oxidative stress in Ehrlich acid solid tumor model induced mice. We used 24 male BALB/c mice divided randomly into three groups. Control (C) group mice were injected intraperitoneally (i.p.) with 0.5 ml saline for four weeks. Tumor (T) group mice were injected i.p. with 0.5 ml saline for four weeks, then Ehrlich acid tumor cells were injected subcutaneously into the neck to induce solid tumor formation...
April 3, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29595063/3-monochloropropane-1-2-diol-alpha-chlorohydrin-disrupts-spermatogenesis-and-causes-spermatotoxicity-in-males-of-the-egyptian-fruit-bat-rousettus-aegyptiacus
#17
Y I Mahmoud, A Taha, S Soliman
We evaluated the sterilizing effect of 3-monochloropropane-1, 2-diol (3-MCPD) in male Egyptian fruit bats (Rousettus aegyptiacus). We used three groups. One was treated with 70 mg/kg 3-MCPD for 4 days. The second group was treated with 3-MCPD as a bait formulation (known concentration of 3-MCPD mixed with a known amount of food). The third group was untreated controls. We compared the weights of the reproductive organs, histology of the testes, occurrence of spermatogenesis, and the count, motility and abnormalities of epididymal sperm of treated males with those of the untreated control group...
March 29, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29575942/effects-of-plantago-lanceolata-l-extract-on-full-thickness-excisional-wound-healing-in-a-mouse-model
#18
B Kurt, N Bilge, M Sözmen, U Aydın, T Önyay, I Özaydın
Wound healing requires cells that increase both collagen production as a result of inflammatory events and regeneration of epithelial tissue. The Plantago species of herbs have been used in traditional treatment of skin disorders and infectious diseases, and digestive, respiratory, reproductive and circulatory conditions. We investigated the efficacy of different concentrations of Plantago lanceolata L. extract (PLE) for wound healing owing to its anti-inflammatory, anti-bacterial, anti-fungal, anti-oxidant, anti-ulcerative, analgesic and immunomodulatory properties...
March 26, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29565181/immunohistochemical-evaluation-of-bcl-2-er-alpha-caspase-3-8-9-pcna-and-ki-67-expressions-in-canine-mammary-carcinomas
#19
A Aydogan, O Ozmen, M Haligur, C Sipahi, D Ileri, A Haligur
We investigated the expression of bcl-2, estrogen receptor alpha (ER-alpha), caspase-3, -8, -9, proliferating cell nuclear antigen (PCNA) and Ki-67 in canine mammary carcinomas. We used 65 paraffin embedded and re-diagnosed archival canine mammary tumor samples to which we applied the routine streptavidin-biotin-peroxidase technique. Seventeen cases were re-diagnosed as tubulopapillary carcinoma, 31 were re-diagnosed as complex carcinoma and 17 were re-diagnosed as carcinosarcoma. Differences of expression of bcl-2 and PCNA were statistically significant according to tumor type...
March 22, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29513110/effects-of-fucoidan-on-diabetic-rat-testicular-tissue
#20
O Ersoy, G Kizilay
Increased reactive oxygen species (ROS) resulting from hyperglycemia and inadequate endogenous antioxidant systems are responsible for the complications of diabetes. ROS accumulate in the cell and stimulate apoptosis, which compromises sperm quality and function. We investigated the possible effects of fucoidan, a potent antioxidant with a regulatory effect on blood glucose homeostasis, on the testicular tissues of rats with experimental diabetes. Diabetes was induced by administering 40 mg/kg streptozotocin (STZ) on five consecutive days...
March 7, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
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