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Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission

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https://www.readbyqxmd.com/read/30411630/corrigendum
#1
(no author information available yet)
No abstract text is available yet for this article.
November 9, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30409033/wound-healing-effects-of-methanol-extract-of-laurocerasus-officinalis-roem
#2
S Ayla, M E Okur, M Y Günal, E M Özdemir, D Çiçek Polat, A Yoltaş, Ö Biçeroğlu, S Karahüseyinoğlu
Laurocerasus officinalis Roem. (syn: Prunus laurocerasus L.) is a member of Rosaceae family. We investigated the antimicrobial and antioxidant activity of L. officinalis Roem in wound healing both in vivo and in vitro using an excisional wound model model in mice. We used four groups of eight mice as follows: untreated (control), empty gel, extract + gel (L. officinalis + gel), and Madecassol® groups. All treatments were applied topically once daily. The scar area, percentage wound closure and epithelization time were measured...
November 9, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30403893/amyloid-from-a-histochemical-perspective-a-review-of-the-structure-properties-and-types-of-amyloid-and-a-proposed-staining-mechanism-for-congo-red-staining
#3
Richard W Dapson
Amyloid is a diverse group of unrelated peptides or proteins that have positive functionality or are associated with various pathologies. Despite vast differences, all amyloids share several features that together uniquely define the group. 1) All amyloids possess a characteristic cross-ß pattern with X-ray diffraction typical of ß-sheet secondary protein structures. 2) All amyloids are birefringent and dichroic under polarizing microscopy after staining with Congo red, which indicates a crystalline-like (ordered) structure...
November 7, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30403883/an-improved-method-for-studying-whole-sections-of-late-developing-wheat-grain
#4
R Zhang, C Li, K Fu, C Li, C Li
Whole sections are important for investigation in situ of the development of cell morphology and accumulation of storage substance in cereal kernels. We developed an improved, simple method to obtain good quality whole histological sections of wheat grains during mid- and late-stage (28 and 35 days post anthesis) grain filling. The wheat grains were fixed and dehydrated, cleared in chloroform for 3 days and infiltrated in paraffin at 55° C for 3 - 5 days. The infiltrated grains then were embedded in paraffin wax, softened using diethyl pyrocarbonate (DEPC) water before sectioning...
November 7, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30392390/application-of-an-optimized-marker-amplification-protocol-in-immunohistochemistry-a-valuable-tool-for-visualizing-antigenic-sites-of-low-signal-strength-or-sparse-distribution
#5
C J von Ruhland
Amplification of immunohistochemical markers received considerable attention during the 1980s and 1990s. The amplification approach was largely abandoned following the development of antigen retrieval and reporter amplification techniques, because the latter were incorporated more easily into high throughput automated procedures in industrial and diagnostic laboratories. There remain, however, a number of instances where marker amplification still has much to offer. Consequently, we examined experimentally the utility of an optimized marker amplification technique in diagnostically relevant tissue where either the original signal strength was low or positive sites were visible, but sparsely distributed...
November 5, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30388897/an-immunohistochemical-study-of-the-diagnostic-value-of-trem-1-as-marker-for-fatal-sepsis-cases
#6
A Maiese, G Bolino, A Mastracchio, P Frati, V Fineschi
Triggering receptor expressed on myeloid cells-1 (TREM-1) is produced and up-regulated by exposure of myeloid cells to lipopolysaccharides or other components of either bacterial or fungal origin, which causes it to be strongly expressed on phagocytes that accumulate in inflamed areas. Because TREM-1 participates in septic shock and in amplifying the inflammatory response to bacterial and fungal infections, we believe it could be an immunohistochemical marker for postmortem diagnosis of sepsis. We tested the anti-TREM-1 antibody in 28 cases of death by septic shock and divided them into two groups...
November 2, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30388896/effects-of-melatonin-on-acetylsalicylic-acid-induced-gastroduodenal-and-jejunal-mucosal-injury
#7
E Taslidere, N Vardi, H Parlakpinar, A Yıldız, B Taslidere, M G Karaaslan
We evaluated the effects of melatonin on acetylsalicylic acid (ASA) induced gastroduodenal and jejunal mucosal injury. We used 40 postpubertal rats divided randomly into five groups of eight animals. The control group consisted of untreated animals. The Mel group was injected intraperitoneally (i.p.) with 5 mg/kg melatonin. The ASA group was injected i.p. with 200 mg/kg ASA. The ASA + Mel group was injected i.p. with 5 mg/kg melatonin 45 min after administering 200 mg/kg ASA i.p. The Mel + ASA group was injected i...
November 2, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30383440/immunohistochemical-examination-of-anti-inflammatory-and-anti-apoptotic-effects-of-hesperetin-on-trinitrobenzene-sulfonic-acid-induced-colitis-in-rats
#8
F R Polat, I Karaboğa
The trinitrobenzene sulfonic acid (TNBS) induced colitis model is used to investigate the pathogenesis of ulcerative colitis. Colon inflammation and apoptosis are associated with tissue damage in ulcerative colitis. Hesperetin is a natural flavonoid that exhibits antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hesperetin on tumor necrosis factor-alpha (TNF-α), protein tyrosine phosphatase, receptor type C (CD45), caspase-3 and Bax expressions in TNBS in induced colitis model in rats...
November 1, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30354692/novel-staining-procedures-for-whole-mount-preparations-of-greater-wax-moth-galleria-mellonella-embryos-toluidine-blue-rhodamine-b-plus-calcofluor-white
#9
Muhamad Abidalla, Donatella Battaglia
Although hematoxylin and eosin (H & E) staining of sectioned embryonic insect material is widely used, it is time-consuming and may not provide sufficient information. We evaluated new staining procedures for embryonic whole mounts of the greater wax moth, Galleria mellonella. We compared a combination of toluidine blue and rhodamine B (TB-RB) to H & E; we also investigated calcofluor white (CFW) staining. TB-RB staining produced staining similar to H & E. TB-RB staining was less time-consuming and improved visualization of the blastoderm and its differentiation into the germ disk and serosa membrane...
October 24, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30354688/impact-of-tissue-preservation-on-collagen-fiber-architecture
#10
H N Hutson, C Kujawa, K Eliceiri, P Campagnola, K S Masters
Microarchitectural features of collagen-rich extracellular matrices provide the mechanical foundation for tissue function and exhibit topographical cues that influence cellular behavior including proliferation, migration and protein expression. Preservation of tissue microarchitecture is required for accurate evaluation of tissue characteristics and pathology. It is unclear whether common tissue preservation methods possess equal ability to preserve microarchitecture. We investigated collagen microarchitecture in samples that had been flash frozen, fixed in formalin or preserved in RNAlater®, and which contained both collagen-rich and collagen-sparse regions...
October 24, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30354531/the-red-insect-dyes-carminic-kermesic-and-laccaic-acids-and-their-derivatives
#11
C J Cooksey
Three groups of insect dyes are described: three cochineal dyes, the kermes dye and the lac dye. The major color components are carminic acid, kermesic acid and laccaic acids, respectively. These dyes are red anthraquinone derivatives. The chemical structures are described. All of these dyes have extensive histories that are related briefly; however, only American cochineal is of commercial importance today. Two manufactured derivatives of cochineal, carmine and acid-stable carmine (4-aminocarminic acid) are described in some detail including the chemical identity, toxicity, stability, and staining and non-staining applications...
October 24, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30354286/correction
#12
(no author information available yet)
No abstract text is available yet for this article.
October 24, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30350720/evaluation-of-apoptosis-markers-in-different-cell-lines-infected-with-equine-arteritis-virus
#13
G E Metz, M M Abeyá, M S Serena, C J Panei, M G Echeverría
Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We evaluated different markers for apoptosis in BHK-21, RK-13 and Vero cell lines using the Bucyrus EAV reference strain. Acridine orange/ethidium bromide staining revealed morphological changes in infected cells, while flow cytometry indicated the extent of apoptosis...
October 23, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30338701/potential-therapeutic-effects-of-induced-pluripotent-stem-cells-on-induced-salivary-gland-cancer-in-experimental-rats
#14
Yasmine Alaa El-Din, Dina Sabry, Amal Hassan Abdelrahman, Safa Fathy
Salivary gland neoplasms exhibit complex histopathology in a variety of tumor types and treatment options depend largely on the stage of the cancer. Induced pluripotent stem cells (iPS) have been investigated for treating induced salivary gland cancer and for restoring salivary gland function. We investigated iPS treatment for salivary gland cancer both in vitro and in vivo. For our study in vitro, we re-programmed human skin fibroblasts to form iPS cells using a plasmid containing Oct4, Sox2, L-MYC and LIN28...
October 19, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30328730/comparison-of-renal-lesions-in-cats-and-dogs-using-pathomorphological-and-%C3%A4-mmunohistochemical-methods
#15
Tuncer Kutlu, Gunay Alcigir
We investigated the pathogenesis of chronic renal fibrosis in cats and dogs using immunohistochemistry. We used the avidin-biotin complex peroxidase (ABC-P) method with antibodies against transforming growth factor-β1, cytokeratin, E-cadherin, S100A4, alpha-smooth muscle actin, vimentin and nestin to determine whether tubule epithelial cells had undergone epithelial-mesenchymal transformation (EMT) that resulted in loss of epithelial cells and an increased number of mesenchymal cells. Although nephrosis was more common in dogs, nephritis was more common in cats; these pathologies developed in both kidneys...
October 17, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30328721/immunoexpression-of-aquaporins-1-2-3-and-4-in-kidney-of-yak-bos-grunniens-on-the-qinghai-tibetan-plateau
#16
Y P Ding, H S Yu, J L Wang, B P Shao
Aquaporins (AQP) 1, 2, 3 and 4 belong to the aquaporin water channel family and play an important role in urine concentration by reabsorption of water from renal tubule fluid. Renal AQPs have not been reported in the yak (Bos grunniens), which resides in the Qinghai Tibetan Plateau. We investigated AQPs 1-4 expressions in the kidneys of Yak using immunohistochemical staining. AQP1 was expressed mainly in the basolateral and apical membranes of the proximal tubules and descending thin limb of the loop of Henle...
October 17, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30317880/thymidine-kinase-1-cd31-double-immunostaining-for-identifying-activated-tumor-vessels
#17
S Okamura, T Osaki, K Nishimura, H Ohsaki, M Shintani, H Matsuoka, K Maeda, K Shiogama, T Itoh, S Kamoshida
Although angiogenesis plays a crucial role in cancer growth and progression, no reliable method for assessing angiogenesis in tumor tissue sections currently is available. Using biomarkers with high specificity for proliferating endothelial cells could help quantify angiogenic activity. Thymidine kinase-1 (TK1) is an enzyme involved in the salvage pathway of DNA synthesis and its activity is correlated with cell proliferation. We investigated the use of double immunostaining for TK1 and CD31 for identifying activated tumor vessels...
October 15, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30317873/dexpanthenol-reduces-diabetic-nephropathy-and-renal-oxidative-stress-in-rats
#18
B Tutun, H Elbe, N Vardi, H Parlakpinar, A Polat, M Gunaltili, M M Guclu, E N Yasar
Hyperglycemia increases reactive oxygen species (ROS) and the resulting oxidative stress contributes to the development of diabetic complications. Dexpanthenol (Dxp) is the biological active form of pantothenic acid. We investigated whether Dxp administration could decrease oxidative stress as a way to treat renal complications of diabetes mellitus (DM). Thirty-two male Wistar albino rats were divided into four groups: control, Dxp, DM and DM + Dxp. Experimental diabetes was induced by a single dose of streptozotocin (STZ)...
October 15, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30317872/effect-of-diabetes-on-collagen-metabolism-and-hypoxia-in-human-gingival-tissue-a-stereological-histopathological-and-immunohistochemical-study
#19
H Balci Yuce, Ö Karatas, F Tulu, A Altan, F Gevrek
Diabetes mellitus and periodontitis are chronic inflammatory diseases that disrupt soft tissue metabolism. The diseases separately or together increase apoptosis in gingival fibroblast cells and reduce cell renewal. We investigated the effects of diabetes and periodontitis on the composition and structure of gingival connective tissue. We used gingival biopsies from 16 healthy individuals (control group, C), 16 type 2 diabetic patients with chronic periodontitis (diabetes + periodontitis group, D + P) and 16 healthy chronic periodontitis patients (periodontitis group, P)...
October 15, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30273072/neuroprotection-against-ccl-4-induced-brain-damage-with-crocin-in-wistar-rats
#20
E Altinoz, M E Erdemli, M Gul, Z Aksungur, S Gul, H G Bag, G B Kaya, Y Turkoz
Owing to its lipophilic property, carbon tetrachloride (CCl4 ) is rapidly absorbed by both the liver and brain. We investigated the protective effects of crocin against brain damage caused by CCl4 . Fifty rats were divided into five groups of ten: control, corn oil, crocin, CCl4 and CCl4 + crocin. CCl4 administration decreased glutathione (GSH) and total antioxidant status (TAS) levels, and catalase (CAT) activity, while significant increases were observed in malondialdehyde (MDA) and total oxidant status (TOS) levels and superoxide dismutase (SOD) activity...
October 1, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
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