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Plant Molecular Biology

Pichang Gong, Jing Li, Chaoying He
Molecular and functional characterization of four gene families of the Physalis exon junction complex (EJC) core improved our understanding of the evolution and function of EJC core genes in plants. The exon junction complex (EJC) plays significant roles in posttranscriptional regulation of genes in eukaryotes. However, its developmental roles in plants are poorly known. We characterized four EJC core genes from Physalis floridana that were named PFMAGO, PFY14, PFeIF4AIII and PFBTZ. They shared a similar phylogenetic topology and were expressed in all examined organs...
November 13, 2018: Plant Molecular Biology
Yutong Liu, Jie Wang, Hao Yin, Ai Zhang, Shuangzhan Huang, Tian-Jing Wang, Qingxiang Meng, Nan Nan, Yifan Wu, Peng Guo, Rafiq Ahmad, Bao Liu, Zheng-Yi Xu
Trithorax-group Protein ARABIDOPSIS TRITHORAX5 modulates the glucose response. Glucose is an evolutionarily conserved modulator from unicellular microorganisms to multicellular animals and plants. Extensive studies have shown that the Trithorax-group proteins (TrxGs) play essential roles in different biological processes by affecting histone modifications and chromatin structures. However, whether TrxGs function in the glucose response and how they achieve the control of target genes in response to glucose signaling in plants remain unknown...
November 8, 2018: Plant Molecular Biology
Hehua Zhang, Xiaoyue Cui, Yuxiao Guo, Chaobing Luo, Lingyun Zhang
Picea wilsonii transcription factor PwNAC2 enhanced plant tolerance to salt and drought stress through multiple signaling pathway and interacted with PwRFCP1 to participate in flowering regulation. NAC is one of the largest transcription factor families in plants, however, its role is not yet fully understood. Here, we identified a transcription factor PwNAC2 in Picea wilsonii, which localized in nucleus with transcriptional activity in C-terminal region and can form homodimer by itself. Expression analysis by real-time PCR showed that PwNAC2 was induced by multiple abiotic stresses and phytohormones stimuli...
November 7, 2018: Plant Molecular Biology
Christoph-Martin Geilfus, Jue Lan, Sebastien Carpentier
Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored triacylglycerols in guard cells to supply ATP for use in stomatal opening. However, proteome changes that happen in the guard cells during dawn were until now poorly understood. Bad accessibility to pure and intact guard cell samples can be considered as the primary reason behind this lack of knowledge...
November 3, 2018: Plant Molecular Biology
Susana Ruiz-Ruiz, Roberta Spanò, Luis Navarro, Pedro Moreno, Leandro Peña, Ricardo Flores
Citrus tristeza virus encodes a unique protein, p23, with multiple functional roles that include co-option of the cytoplasmic glyceraldehyde 3-phosphate dehydrogenase to facilitate the viral infectious cycle. The genome of citrus tristeza virus (CTV), genus Closterovirus family Closteroviridae, is a single-stranded (+) RNA potentially encoding at least 17 proteins. One (p23), an RNA-binding protein of 209 amino acids with a putative Zn-finger and some basic motifs, displays singular features: (i) it has no homologues in other closteroviruses, (ii) it accumulates mainly in the nucleolus and Cajal bodies, and in plasmodesmata, and (iii) it mediates asymmetric accumulation of CTV RNA strands, intracellular suppression of RNA silencing, induction of some CTV syndromes and enhancement of systemic infection when expressed as a transgene ectopically or in phloem-associated cells in several Citrus spp...
November 3, 2018: Plant Molecular Biology
Zhaowan Shi, Tamar Halaly-Basha, Chuanlin Zheng, Mira Weissberg, Ron Ophir, David W Galbraith, Xuequn Pang, Etti Or
Transient increases in ethylene biosynthesis, achieved by tight regulation of transcription of specific ACC oxidase and ACC synthase genes, play a role in activation of grapevine bud dormancy release. The molecular mechanisms regulating dormancy release in grapevine buds are as yet unclear. It has been hypothesized that its core involves perturbation of respiration which induces an interplay between ethylene and ABA metabolism that removes repression and allows regrowth. Roles for hypoxia and ABA metabolism in this process have been previously supported...
November 3, 2018: Plant Molecular Biology
Qing Liu, Shijuan Yan, Wenjie Huang, Jianyuan Yang, Jingfang Dong, Shaohong Zhang, Junliang Zhao, Tifeng Yang, Xingxue Mao, Xiaoyuan Zhu, Bin Liu
This is the first time to dissect the mechanism of NACs-mediated disease resistance in plants using metabolomic approach and discover the involvement of ABA signaling pathway in NACs-mediated disease resistance. NAC transcription factors have been validated as important regulators in stress responses, but their molecular mechanisms in plant disease resistance are still largely unknown. Here we report that the NAC gene ONAC066 (LOC_Os01g09550) is significantly activated by rice blast infection. ONAC066 is ubiquitously expressed and this protein is localized in the nucleus...
November 1, 2018: Plant Molecular Biology
Thula Sravankumar, Akash, NandKiran Naik, Rahul Kumar
Silencing of SlGH3-2 in tomato alters auxin and ethylene levels during fruit ripening and cause reduced lycopene accumulation in the transgenic fruits. While auxin's role during fleshy fruit ripening is widely acknowledged to be important, the physiological functions of several ripening-induced genes, especially those involved in the maintenance of cellular auxin homeostasis, largely remain under-explored. In the present study, the updated inventory shows that 24 members constitute the Gretchen-Hagen 3 (GH3) gene family in tomato...
October 26, 2018: Plant Molecular Biology
Fumio Takaiwa, Yuko Ogo, Yuhya Wakasa
Specific domain of the Mal d 1 was identified to be mainly involved in higher accumulation level in vegetative tissues of transgenic rice than the Bet v 1. Apple food allergen Mal d 1 and birch pollen allergen Bet v 1 belong to the same pathogen related protein 10 (PR10) family. When green fluorescent protein (GFP) fused to either of these allergens was expressed as a secretory protein in transgenic rice by ligating an N terminal signal peptide and a C terminal KDEL ER retention signal under the control of the maize ubiquitin constitutive promoter, the GFP:Mald1 highly accumulated in various tissues, whereas accumulation level of the GFP:Betv1 was remarkably reduced in vegetative tissues except for seed...
October 22, 2018: Plant Molecular Biology
Yijun Wang, Yali Wang, Jia Zhao, Jiayu Huang, Yining Shi, Dexiang Deng
We report coding and long noncoding RNAs in maize upon phytohormone gibberellin stimulation. Plant hormone gibberellin (GA) orchestrates various facets of biological processes. Dissection the transcriptomic dynamics upon GA stimulation has biological significance. Feature of maize transcriptome in response to GA application remains largely elusive. Herein, two types of plants, one was with normal height, the other was GA-sensitive dwarfism, were selected from advanced backcross population for GA3 treatment with different concentrations...
October 19, 2018: Plant Molecular Biology
Teresa Faddetta, Loredana Abbate, Giovanni Renzone, Antonio Palumbo Piccionello, Antonella Maggio, Elisabetta Oddo, Andrea Scaloni, Anna Maria Puglia, Giuseppe Gallo, Francesco Carimi, Sergio Fatta Del Bosco, Francesco Mercati
Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded traits into cultivated varieties. In these cases, the plant coordinated array of physiological, biochemical, and molecular functions remains the result of integration among different signals, which derive from the compartmentalized genomes of nucleus, plastids and mitochondria...
October 19, 2018: Plant Molecular Biology
Bobin Liu, Jin Zhang, Zhaohe Yang, Akihiro Matsui, Motoaki Seki, Shubin Li, Xinyang Yan, Markus V Kohnen, Lianfeng Gu, Kalika Prasad, Gerald A Tuskan, Mengzhu Lu, Yoshito Oka
WUSCHEL-RELATED HOMEOBOX 11 establishes the acquisition of pluripotency during callus formation and accomplishes de novo shoot formation by regulating key transcription factors in poplar. De novo shoot regeneration is a prerequisite for propagation and genetic engineering of elite cultivars in forestry. However, the regulatory mechanism of de novo organogenesis is poorly understood in tree species. We previously showed that WUSCHEL (WUS)-RELATED HOMEOBOX 11 (PtWOX11) of the hybrid poplar clone 84K (Populus alba × P...
October 15, 2018: Plant Molecular Biology
Sandeep J Sarde, Abhishek Kumar, Rahima N Remme, Marcel Dicke
Lipoxygenases mediate important biological processes. Through comparative genomics, domain-scan analysis, sequence analysis, phylogenetic analysis, homology modelling and transcriptional analysis the lipoxygenase gene family of pepper (Capsicum annuum) has been identified. Lipoxygenases (LOXs) are non-heme, iron-containing dioxygenases playing a pivotal role in diverse biological processes in plants, including defence and development. Here, we exploited the recent sequencing of the pepper genome to investigate the LOX gene family in pepper...
October 13, 2018: Plant Molecular Biology
Hui Song, Juan Sun, Guofeng Yang
Our results reveal that Ks is a determining factor affecting selective pressure and different evolution and expression patterns are detected between PSGs and NSGs in wild Arachis duplicates. Selective pressure, including purifying (negative) and positive selection, can be detected in organisms. However, studies on comparative evolutionary rates, gene expression patterns and gene features between negatively selected genes (NSGs) and positively selected genes (PSGs) are lagging in paralogs of plants. Arachis duranensis and Arachis ipaënsis are ancestors of the cultivated peanut, an important oil and protein crop...
October 8, 2018: Plant Molecular Biology
Gui-Li Yang, Yang Fang, Ya-Liang Xu, Li Tan, Qi Li, Yang Liu, Fan Lai, Yan-Ling Jin, An-Ping Du, Kai-Ze He, Xin-Rong Ma, Hai Zhao
The Lemnaceae, known as duckweed, the smallest flowering aquatic plant, shows promise as a plant bioreactor. For applying this potential plant bioreactor, establishing a stable and efficient genetic transformation system is necessary. The currently favored callus-based method for duckweed transformation is time consuming and genotype limited, as it requires callus culture and regeneration, which is inapplicable to many elite duckweed strains suitable for bioreactor exploitation. In this study, we attempted to establish a simple frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor, one of the most widespread duckweed species in the world...
October 8, 2018: Plant Molecular Biology
Sarah Al-Sheikh Ahmed, Jingjuan Zhang, Wujun Ma, Bernard Dell
The homologous genes to OsSUT1-5 in wheat were identified and detailed analysed. TaSUT1 was the predominant sucrose transporter group and it illustrated the genotypic variations towards drought during grain filling. Sucrose transporters (SUT) play crucial roles in wheat stem water soluble carbohydrate (WSC) remobilization to grain. To determine the major functional SUT gene groups in shoot parts of wheat during grain development, drought tolerant varieties, Westonia and Kauz, were investigated in field drought experiments...
October 4, 2018: Plant Molecular Biology
Karla S Macedo-Osorio, Víctor H Pérez-España, Claudio Garibay-Orijel, Daniel Guzmán-Zapata, Noé V Durán-Figueroa, Jesús A Badillo-Corona
Two intercistronic regions were identified as functional intercistronic expression elements (IEE) for the simultaneous expression of aphA-6 and gfp in a synthetic operon in the chloroplast of C. reinhardtii. Chlamydomonas reinhardtii, a biflagellate photosynthetic microalga, has been widely used in basic and applied science. Already three decades ago, Chlamydomonas had its chloroplast genome transformed and to this day constitutes the only alga routinely used in transplastomic technology. Despite the fact that over a 100 foreign genes have been expressed from the chloroplast genome, little has been done to address the challenge of expressing multiple genes in the form of operons, a development that is needed and crucial to push forward metabolic engineering and synthetic biology in this organism...
September 17, 2018: Plant Molecular Biology
Hengsheng Wang, Longjiang Gu, Xingen Zhang, Mingli Liu, Haiyang Jiang, Ronghao Cai, Yang Zhao, Beijiu Cheng
Weighted gene co-expression network analysis was explored to find key hub genes involved in plant height regulation. Plant height, an important trait for maize breeding because of its close relatedness to lodging resistance and yield, has been reported to be determined by multiple qualitative and quantitative genes. However, few genes related to plant height have been characterized in maize. Herein, three different maize hybrids, with extremely distinct plant height, which were further classified into low (L), middle (M) and high (H) group, were selected for RNA sequencing at three key developmental stages, namely, jointing stage (I), big flare period (II) and tasseling stage (III)...
October 2018: Plant Molecular Biology
Guofang Li, Ming Tan, Fang Cheng, Xiaojie Liu, Siyan Qi, Hongfei Chen, Dong Zhang, Caiping Zhao, Mingyu Han, Juanjuan Ma
Axillary bud activation and outgrowth were dependent on local cytokinin, and that bud activation preceded the activation of cell cycle and cell growth genes in apple branching. Cytokinin is often applied to apple trees to produce more shoot branches in apple seedlings. The molecular response of apple to the application of cytokinin, and the relationship between bud activation and cell cycle in apple branching, however, are poorly understood. In this study, RNA sequencing was used to characterize differential expression genes in axillary buds of 1-year grafted "Fuji" apple at 4 and 96 h after cytokinin application...
October 2018: Plant Molecular Biology
Wenbo Li, Haimin Li, Peng Xu, Zhi Xie, Yajin Ye, Lingting Li, Deqiang Li, Yijing Zhang, Laigeng Li, Yang Zhao
A new synthetic auxin AAL1 with new structure was identified. Different from known auxins, it has weak effects. By AAL1, we found specific amino acids could restore the effects of auxin with similar structure. Auxin, one of the most important phytohormones, plays crucial roles in plant growth, development and environmental response. Although many critical regulators have been identified in auxin signaling pathway, some factors, especially those with weak fine-tuning roles, are still yet to be discovered. Through chemical genetic screenings, we identified a small molecule, Auxin Activity Like 1 (AAL1), which can effectively inhibit dark-grown Arabidopsis thaliana seedlings...
October 2018: Plant Molecular Biology
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