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Plant Molecular Biology

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https://www.readbyqxmd.com/read/28639116/integration-of-transcriptomic-and-metabolic-data-reveals-hub-transcription-factors-involved-in-drought-stress-response-in-sunflower-helianthus-annuus-l
#1
Sebastián Moschen, Julio A Di Rienzo, Janet Higgins, Takayuki Tohge, Mutsumi Watanabe, Sergio González, Máximo Rivarola, Francisco García-García, Joaquin Dopazo, H Esteban Hopp, Rainer Hoefgen, Alisdair R Fernie, Norma Paniego, Paula Fernández, Ruth A Heinz
By integration of transcriptional and metabolic profiles we identified pathways and hubs transcription factors regulated during drought conditions in sunflower, useful for applications in molecular and/or biotechnological breeding. Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach...
June 21, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28634865/dynamics-of-tobacco-dna-topoisomerases-ii-in-cell-cycle-regulation-to-manage-topological-constrains-during-replication-transcription-and-mitotic-chromosome-condensation-and-segregation
#2
Badri Nath Singh, V Mohan Murali Achary, Varakumar Panditi, Sudhir K Sopory, Malireddy K Reddy
The topoisomerase II expression varies as a function of cell proliferation. Maximal topoisomerase II expression was tightly coupled to S phase and G2/M phase via both transcriptional and post-transcriptional regulation. Investigation in meiosis using pollen mother cells also revealed that it is not the major component of meiotic chromosomes, it seems to diffuse out once meiotic chromosomal condensation is completed. Synchronized tobacco BY-2 cell cultures were used to study the role of topoisomerase II in various stages of the cell cycle...
June 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28631168/the-function-of-osbhlh068-is-partially-redundant-with-its-homolog-atbhlh112-in-the-regulation-of-the-salt-stress-response-but-has-opposite-functions-to-control-flowering-in-arabidopsis
#3
Hung-Chi Chen, Vicki Hsieh-Feng, Pei-Chun Liao, Wan-Hsing Cheng, Li-Yu Liu, Yun-Wei Yang, Ming-Hsin Lai, Men-Chi Chang
The homologous genes OsbHLH068 and AtbHLH112 have partially redundant functions in the regulation of the salt stress response but opposite functions to control flowering in Arabidopsis. The transcription factor (TF) basic/Helix-Loop-Helix (bHLH) is important for plant growth, development, and stress responses. OsbHLH068, which is a homologous gene of AtbHLH112 that is up-regulated under drought and salt stresses, as indicated by previous microarray data analysis. However, the intrinsic function of OsbHLH068 remains unknown...
June 19, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28631167/copper-and-ectopic-expression-of-the-arabidopsis-transport-protein-copt1-alter-iron-homeostasis-in-rice-oryza-sativa-l
#4
Amparo Andrés-Bordería, Fernando Andrés, Antoni Garcia-Molina, Ana Perea-García, Concha Domingo, Sergi Puig, Lola Peñarrubia
Copper deficiency and excess differentially affect iron homeostasis in rice and overexpression of the Arabidopsis high-affinity copper transporter COPT1 slightly increases endogenous iron concentration in rice grains. Higher plants have developed sophisticated mechanisms to efficiently acquire and use micronutrients such as copper and iron. However, the molecular mechanisms underlying the interaction between both metals remain poorly understood. In the present work, we study the effects produced on iron homeostasis by a wide range of copper concentrations in the growth media and by altered copper transport in Oryza sativa plants...
June 19, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28608281/suppression-of-cucumber-stachyose-synthase-gene-cssts-inhibits-phloem-loading-and-reduces-low-temperature-stress-tolerance
#5
Jianguo Lü, Xiaolei Sui, Si Ma, Xin Li, Huan Liu, Zhenxian Zhang
Stachyose is the main transporting sugar in phloem of Raffinose family oligosaccharides-transporting species. Stachyose synthase (STS) is a key enzyme for stachyose biosynthesis, but the gene encoding STS is poorly characterized in cucumber (Cucumis sativus L.), which is a model plant for studying stachyose metabolism and phloem function. In this research, stachyose synthase gene (CsSTS) from cucumber was isolated and its physiological functions were analyzed. CsSTS expressed mainly in the phloem of the minor veins in mature leaves and localized to companion cells...
June 12, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28578496/thdof1-4-and-thzfp1-constitute-a-transcriptional-regulatory-cascade-involved-in-salt-or-osmotic-stress-in-tamarix-hispida
#6
Dandan Zang, Lina Wang, Yiming Zhang, Huimin Zhao, Yucheng Wang
Identification of the upstream regulators of a gene is important to characterize the transcriptional pathway and the function of the gene. Previously, we found that a zinc finger protein (ThZFP1) is involved in abiotic stress tolerance of Tamarix hispida. In the present study, we further investigated the transcriptional pathway of ThZFP1. Dof motifs are abundant in the ThZFP1 promoter; therefore, we used them to screen for transcriptional regulators of ThZFP1. A Dof protein, ThDof1.4, binds to the Dof motif specifically, and was hypothesized as the upstream regulator of ThZFP1...
June 3, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28551765/mir529a-modulates-panicle-architecture-through-regulating-squamosa-promoter-binding-like-genes-in-rice-oryza-sativa
#7
Erkui Yue, Chao Li, Yu Li, Zhen Liu, Jian-Hong Xu
MiR529a affects rice panicle architecture by targeting OsSPL2,OsSPL14 and OsSPL17 genes that could regulate their downstream panicle related genes. The panicle architecture determines the grain yield and quality of rice, which could be regulated by many transcriptional factors. The SQUAMOSA PROMOTER BINDING-LIKE (SPL) transcription factors are involved in the regulation of panicle development, which are targeted by miR156 and miR529. The expression profile demonstrated that miR529a is preferentially expressed in the early panicle of rice and it might regulate panicle development in rice...
May 27, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28540497/large-scale-transcriptome-analysis-reveals-arabidopsis-metabolic-pathways-are-frequently-influenced-by-different-pathogens
#8
Zhenhong Jiang, Fei He, Ziding Zhang
Through large-scale transcriptional data analyses, we highlighted the importance of plant metabolism in plant immunity and identified 26 metabolic pathways that were frequently influenced by the infection of 14 different pathogens. Reprogramming of plant metabolism is a common phenomenon in plant defense responses. Currently, a large number of transcriptional profiles of infected tissues in Arabidopsis (Arabidopsis thaliana) have been deposited in public databases, which provides a great opportunity to understand the expression patterns of metabolic pathways during plant defense responses at the systems level...
May 24, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28536883/identification-of-genes-related-to-skin-development-in-potato
#9
Vijaya K R Vulavala, Edna Fogelman, Lior Rozental, Adi Faigenboim, Zachariah Tanami, Oded Shoseyov, Idit Ginzberg
Newly identified genes that are preferentially expressed in potato skin include genes that are associated with the secondary cell wall and stress-related activities and contribute to the skin's protective function. Microarrays were used to compare the skin and tuber-flesh transcriptomes of potato, to identify genes that contribute to the unique characteristics of the skin as a protective tissue. Functional gene analysis indicated that genes involved in developmental processes such as cell division, cell differentiation, morphogenesis and secondary cell wall formation (lignification and suberization), and stress-related activities, are more highly expressed in the skin than in the tuber flesh...
May 23, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28508171/over-expression-of-tobacco-ubc1-encoding-a-ubiquitin-conjugating-enzyme-increases-cadmium-tolerance-by-activating-the-20s-26s-proteasome-and-by-decreasing-cd-accumulation-and-oxidative-stress-in-tobacco-nicotiana-tabacum
#10
Ramin Bahmani, DongGwan Kim, Byoung Doo Lee, Seongbin Hwang
Ubiquitin (Ub)-conjugating enzyme (UBC, E2) receives Ub from Ub-activating enzyme (E1) and transfers it to target proteins, thereby playing a key role in Ub/26S proteasome-dependent proteolysis. UBC has been reported to be involved in tolerating abiotic stress in plants, including drought, salt, osmotic and water stresses. To isolate the genes involved in Cd tolerance, we transformed WT (wild-type) yeast Y800 with a tobacco cDNA expression library and isolated a tobacco cDNA, NtUBC1 (Ub-conjugating enzyme), that enhances cadmium tolerance...
May 15, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28466433/differences-in-specificity-and-compensatory-functions-among-three-major-starch-synthases-determine-the-structure-of-amylopectin-in-rice-endosperm
#11
Naoko Crofts, Kyohei Sugimoto, Naoko F Oitome, Yasunori Nakamura, Naoko Fujita
The lengths of amylopectin-branched chains are precise and influence the physicochemical properties of starch, which determine starch functionality. Three major isozymes of starch synthases (SSs), SSI, SSII(a), and SSIII(a), are primarily responsible for amylopectin chain elongation in the storage tissues of plants. To date, the majority of reported rice mutants were generated using japonica cultivars, which have almost inactive SSIIa. Although three SSs share some overlapping chain length preferences, whether they complement each other remains unknown due to the absence of suitable genetic combinations of materials...
May 2, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28455648/overexpression-of-psp1-enhances-growth-of-transgenic-arabidopsis-plants-under-ambient-air-conditions
#12
Xiaofang Han, Keli Peng, Haixia Wu, Shanshan Song, Yerong Zhu, Yanling Bai, Yong Wang
The importance of the phosphorylated pathway (PPSB) of L-serine (Ser) biosynthesis in plant growth and development has been demonstrated, but its specific role in leaves and interaction with photorespiration, the main leaf Ser biosynthetic pathway at daytime, are still unclear. To investigate whether changes in biosynthesis of Ser by the PPSB in leaves could have an impact on photorespiration and plant growth, we overexpressed PSP1, the last enzyme of this pathway, under control of the Cauliflower Mosaic Virus 35S promoter in Arabidopsis thaliana...
April 28, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28444544/methylglyoxal-detoxification-by-a-dj-1-family-protein-provides-dual-abiotic-and-biotic-stress-tolerance-in-transgenic-plants
#13
Prasad Melvin, Kondalarao Bankapalli, Patrick D'Silva, P V Shivaprasad
Methylglyoxal (MG) is a key signaling molecule resulting from glycolysis and other metabolic pathways. During abiotic stress, MG levels accumulate to toxic levels in affected cells. However, MG is routinely detoxified through the action of DJ1/PARK7/Hsp31 proteins that are highly conserved across kingdoms and mutations in such genes are associated with neurodegenerative diseases. Here, we report for the first time that, similar to abiotic stresses, MG levels increase during biotic stresses in plants, likely contributing to enhanced susceptibility to a wide range of stresses...
April 25, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28434151/cbf2a-cbf4b-genomic-region-copy-numbers-alongside-the-circadian-clock-play-key-regulatory-mechanisms-driving-expression-of-fr-h2-cbfs
#14
Taniya Dhillon, Kengo Morohashi, Eric J Stockinger
The C-Repeat Binding Factors (CBFs) are DNA-binding transcriptional activators that were identified using Arabidopsis thaliana. In barley, Hordeum vulgare, a cluster of CBF genes reside at FROST RESISTANCE-H2, one of two loci having major effects on winter-hardiness. FR-H2 was revealed in a population derived from the winter barley 'Nure' and the spring barley 'Trèmois'. 'Nure' harbors two to three copies of CBF2A and CBF4B as a consequence of tandem iteration of the genomic region encompassing these genes whereas 'Trèmois' harbors single copies, and these copy number differences are associated with their transcript level differences...
April 22, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28429252/analysis-of-global-gene-expression-profiles-during-the-flowering-initiation-process-of-lilium%C3%A2-%C3%A3-%C3%A2-formolongi
#15
Yu-Fan Li, Ming-Fang Zhang, Meng Zhang, Gui-Xia Jia
The onset of flowering is critical for the reproductive development of plants. Lilium × formolongi is a lily hybrid that flowers within a year after sowing. We successfully identified four important stages during vegetative growth and flowering initiation of L. × formolongi under long day conditions. The plant tissues from the four stages were used in a genome-wide transcriptional analysis to investigate stage-specific changes of gene expression in L. × formolongi. In total, the sequence reads of the four RNA-sequencing libraries were assembled into 52,824 unigenes, of which 37,031 (70...
April 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28424966/transcriptomic-analysis-of-molecular-responses-in-malus-domestica-m26-roots-affected-by-apple-replant-disease
#16
Stefan Weiß, Melanie Bartsch, Traud Winkelmann
Gene expression studies in roots of apple replant disease affected plants suggested defense reactions towards biotic stress to occur which did not lead to adequate responses to the biotic stressors. Apple replant disease (ARD) leads to growth inhibition and fruit yield reduction in replanted populations and results in economic losses for tree nurseries and fruit producers. The etiology is not well understood on a molecular level and causal agents show a great diversity indicating that no definitive cause, which applies to the majority of cases, has been found out yet...
April 19, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28409321/an-integrative-overview-of-the-molecular-and-physiological-responses-of-sugarcane-under-drought-conditions
#17
Camilo Elber Vital, Andrea Giordano, Eduardo de Almeida Soares, Thomas Christopher Rhys Williams, Rosilene Oliveira Mesquita, Pedro Marcus Pereira Vidigal, Amanda de Santana Lopes, Túlio Gomes Pacheco, Marcelo Rogalski, Humberto Josué de Oliveira Ramos, Marcelo Ehlers Loureiro
Drought is the main abiotic stress constraining sugarcane production. However, our limited understanding of the molecular mechanisms involved in the drought stress responses of sugarcane impairs the development of new technologies to increase sugarcane drought tolerance. Here, an integrated approach was performed to reveal the molecular and physiological changes in two closely related sugarcane cultivars, including the most extensively planted cultivar in Brazil (cv. RB867515), in response to moderate (-0.5 MPa) and severe (-1 MPa) drought stress at the transcriptional, translational, and posttranslational levels...
April 13, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28405784/genomic-and-functional-characterization-of-coleopteran-insect-specific-%C3%AE-amylase-inhibitor-gene-from-amaranthus-species
#18
Amey J Bhide, Sonal M Channale, Yashpal Yadav, Kabita Bhattacharjee, Pankaj K Pawar, V L Maheshwari, Vidya S Gupta, Sureshkumar Ramasamy, Ashok P Giri
The smallest 32 amino acid α-amylase inhibitor from Amaranthus hypochondriacus (AAI) is reported. The complete gene of pre-protein (AhAI) encoding a 26 amino acid (aa) signal peptide followed by the 43 aa region and the previously identified 32 aa peptide was cloned successfully. Three cysteine residues and one disulfide bond conserved within known α-amylase inhibitors were present in AhAI. Identical genomic and open reading frame was found to be present in close relatives of A. hypochondriacus namely Amaranthus paniculatus, Achyranthes aspera and Celosia argentea...
April 12, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28365837/comparison-of-phytohormone-levels-and-transcript-profiles-during-seasonal-dormancy-transitions-in-underground-adventitious-buds-of-leafy-spurge
#19
Wun S Chao, Münevver Doğramacı, David P Horvath, James V Anderson, Michael E Foley
Leafy spurge (Euphorbia esula L.) is an herbaceous perennial weed that maintains its perennial growth habit through generation of underground adventitious buds (UABs) on the crown and lateral roots. These UABs undergo seasonal phases of dormancy under natural conditions, namely para-, endo-, and ecodormancy in summer, fall, and winter, respectively. These dormancy phases can also be induced in growth chambers by manipulating photoperiod and temperature. In this study, UABs induced into the three phases of dormancy under controlled conditions were used to compare changes in phytohormone and transcriptome profiles...
April 1, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28364390/in-silico-analysis-of-the-sequence-features-responsible-for-alternatively-spliced-introns-in-the-model-green-alga-chlamydomonas-reinhardtii
#20
Praveen-Kumar Raj-Kumar, Olivier Vallon, Chun Liang
Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternative splicing (AS) in plants from an evolutionary perspective as it diverged from land plants a billion years ago. Using over 7 million cDNA sequences from both pyrosequencing and Sanger sequencing, we found that a much higher percentage of genes (~20% of multi-exon genes) undergo AS than previously reported (3-5%)...
March 31, 2017: Plant Molecular Biology
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