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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/30404955/effect-of-fisetin-and-probiotic-supplementation-on-erythrocyte-osmotic-fragility-malondialdehyde-concentration-and-superoxide-dismutase-activity-in-broiler-chickens-exposed-to-heat-stress
#1
Ngozi Ejum Ogbuagu, Tagang Aluwong, Joseph Olusegun Ayo, Victory Osirimade Sumanu
The aim of the study was to evaluate effects of fisetin and probiotic on erythrocyte osmotic fragility (EOF), malondialdehyde (MDA) and superoxide dismutase (SOD) in broiler chickens exposed to heat stress. Sixty day-old broilers were divided into: Group I (control) given distilled water; Group II, fisetin (5 mg/kg); Group III, probiotic Saccharomyces cerevisiae (4.125 × 106 cfu/100 ml); and Group IV, fisetin (5 mg/kg) + probiotic (4.125 × 106 cfu/100 ml) orally for 7 days. Blood samples collected from 42-day-old birds were evaluated for EOF, serum MDA concentration and SOD activity...
November 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30404954/a-case-of-pulmonary-toxoplasmosis-resembling-multiple-lung-metastases-of-nasal-lymphoma-in-a-cat-receiving-chemotherapy
#2
Mami Murakami, Takashi Mori, Yasuhiro Takashima, Kisaburo Nagamune, Junpei Fukumoto, Katsuya Kitoh, Hiroki Sakai, Kohji Maruo
An 11-year-old cat presented with nasal discharge and lacrimation and was diagnosed with nasal lymphoma. Although the cat showed favorable progression after undergoing chemotherapy, CT imaging demonstrated enlarged pulmonary nodules caused by Toxoplasma gondii. Following the cessation of chemotherapy, the cat was prescribed clindamycin hydrochloride for toxoplasmosis treatment; however, the cat developed kidney lymphoma and died. No T. gondii organisms were observed in the whole body necropsy specimens. It is known that immunocompromised human patients, including those who undergo chemotherapy, are considered at risk for toxoplasmosis...
November 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30404953/infiltrative-lipoma-causing-vertebral-deformation-and-spinal-cord-compression-in-a-dog
#3
Shintaro Kimura, Midori Yamazaki, Manabu Tomohisa, Takashi Mori, Tokuma Yanai, Sadatoshi Maeda, Hiroaki Kamishina
A 4-year-old, male Bernese mountain dog was evaluated for a 1-year history of right hemiparesis. Computed tomography revealed a large hypoattenuating mass severely deforming the C5 vertebral arch, invading the C6 spinal canal, and causing spinal cord compression. The signal characteristics of magnetic resonance imaging indicated a lesion composed of adipose tissue. The mass was removed via right hemilaminectomy, and histopathological examination confirmed it was an infiltrative lipoma. The compressive lesion remained unresolved, so the dog underwent a second operation, after which he regained some ambulatory function...
November 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30404952/inotropic-effects-of-a-single-intravenous-recommended-dose-of-pimobendan-in-healthy-dogs
#4
Yasutomo Hori, Hiroto Taira, Yuji Nakajima, Yusyun Ishikawa, Yuki Yumoto, Yuya Maekawa, Akiko Oshiro
We investigated the effects of an injectable pimobendan solution (0.15 mg/kg) on cardiac function in healthy dogs. Fifteen dogs were divided into placebo, intravenous pimobendan injection, and subcutaneous pimobendan injection groups. In the placebo, the heart rate, systolic and end-diastolic left ventricular pressure (LVPs and LVEDP), and peak positive (max dP/dt) and negative (min dP/dt) first derivatives of the left ventricular pressure did not change for 60 min. After the intravenous pimobendan injection, LVEDP decreased significantly within 5 min, while the max dP/dt increased, and the effects continued until 60 min...
November 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30404975/the-effect-of-colostrum-intake-on-osteoprotegerin-and-bone-metabolic-markers-in-the-blood-of-newborn-calves-during-the-first-week-of-life
#5
Kaoru Hatate, Mitsunori Kayano, Chiho Kawashima, Masaaki Hanada, Rodrigo C Bicalho, Norio Yamagishi
The aim of this study was to elucidate the bone metabolic status after taking colostrum in newborn calves. Fourteen neonatal calves were randomly allocated to two groups fed either unheated or heated (60°C, 30 min) colostrum three times on the first day (2 l every 10 hr; 6 l in total). Heat treatment on colostrum was to reduce the bone metabolic markers assumed as heat-sensitive. The concentrations of four bone metabolic markers (the enzymes from bone cells or the bone collagen fragments) and a bone protective protein, osteoprotegerin (OPG), were measured in the blood of calves during a week after the birth and in the colostrum...
November 7, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30404994/basal-cell-adenocarcinoma-on-bulbar-conjunctiva-of-third-eyelid-in-a-dog
#6
Yuto Sano, Mai Miyazaki, Rina Yaegashi, Minoru Okamoto, Arisa Masuko, Seiya Maehara, Kazuya Matsuda
An 8-year-old castrated Toy poodle presented with swelling and proptosis of the right third eyelid caused by an exophytic mass on the bulbar surface. Histologically, the mass was composed of stratified neoplastic basaloid cells, arranged in nests and interconnecting islands, which were mixed with tubular structures. Immunohistochemically, the basaloid cells were positive for p63 and cytokeratin (CK) 14, and the inner epithelial cells of the tubular structures were positive for CK7, CK8, and CK19. According to these findings, the mass was diagnosed as a basal cell adenocarcinoma...
November 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30393268/detection-of-%C3%AE-defensin-in-eosinophils-in-helminth-infected-mouse-model
#7
Afia Khatun, Masashi Sakurai, Kazuki Okada, Yusuke Sakai, Masahiro Morimoto
α-defensin is a potent antimicrobial peptide secreted from intestinal mucosal epithelial cells, such as Paneth cells, and affects not only bacteria but also parasites and fungi. Recently, human eosinophils have also been shown to produce α-defensin, but no studies have been done on other animals. In this study, we attempted to detect α-defensin protein in mouse eosinophils infiltrating the intestinal mucosa during a helminth infection using Zamboni fixation and immunohistochemistry. Most of the eosinophils infiltrating the intestinal mucosa during helminth infection were positive for α-defensin...
November 5, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30393267/captive-wildlife-from-india-as-carriers-of-shiga-toxin-producing-enteropathogenic-and-enterotoxigenic-escherichia-coli
#8
Arockiasamy Arun Prince Milton, Rajesh Kumar Agarwal, Govindarajan Bhuvana Priya, Manivasagam Aravind, Cheruplackal Karunakaran Athira, Losa Rose, Mani Saminathan, Anil Kumar Sharma, Ashok Kumar
Shiga toxin-producing Escherichia coli (STEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC) make up an important group of pathogens causing major animal and public health concerns worldwide. The aim of this study was to determine the prevalence of different pathotypes of E. coli in captive wildlife. We analyzed 314 fresh fecal samples from captive wildlife, 30 stool swabs from animal caretakers, and 26 feed and water samples collected from various zoological gardens and enclosures in India for the isolation of E...
November 2, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30381675/bioequivalence-evaluation-of-two-5-ceftiofur-hydrochloride-sterile-suspension-in-pigs
#9
Jincheng Xiong, Qianqian Zhu, Zhixin Lei, Shuaike Yang, Peiyuan Chen, Yaxin Zhao, Jiyue Cao, Yinsheng Qiu
The purpose of this study was to evaluate the bioequivalence of 5% ceftiofur hydrochloride sterile suspension in two formulations, a test formulation (Saifukang 5% CEF, Hvsen) and a reference formulation (Excenel® RTU 5% CEF, Pfizer). Twenty-four healthy pigs were assigned to a two-period, two-treatment crossover parallel trial, and both formulations were administered at a single intramuscular dose of 5 mg/kg weight, with a 7-day washout period. Blood samples were collected consecutively for up to 144 hr after administration...
November 1, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30369588/identification-of-moraxella-lacunata-from-pulmonary-abscesses-in-three-zoo-herbivores
#10
Kyoo-Tae Kim, Seung-Hun Lee, Dongmi Kwak
Although Moraxella lacunata causes conjunctivitis, keratitis, endocarditis, and otolaryngitis in humans, its infection is rare in animals. We report three cases of asymptomatic pulmonary abscesses caused by M. lacunata in zoo herbivores, including two elks (Cervus canadensis) and a common eland (Taurotragus oryx). In all cases, macroscopic findings included coalescence of lung lobes and severe pulmonary abscesses filled with cheese-like materials in cysts. Microscopic findings included pneumonia characterized by marked fibrin exudates in alveolar spaces and infiltration of inflammatory cells...
October 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30369587/an-epidemiological-analysis-of-the-level-of-biosecurity-and-animal-welfare-on-pig-farms-in-japan-and-their-effect-on-the-use-of-veterinary-antimicrobials
#11
Ren Isomura, Mari Matsuda, Katsuaki Sugiura
In Japan the highest use of veterinary antimicrobials is in pig production. To obtain useful information to achieve the best approach to reducing this use, we analyzed the association between the level of on-farm biosecurity and animal welfare with the level of antimicrobial use as recorded on prescriptions on 38 pig farms under contract to veterinarians of the Japanese Association of Swine Veterinarians. To determine the level of welfare we recorded the risk of pre- and post-weaning deaths and the floor space available per fattening pig (m2 /head)...
October 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30369586/a-case-of-anthrax-in-two-captive-pumas-puma-concolor
#12
Gorkem Ekebas, Ayhan Atasever, Duygu Yaman Gram, Emre Karakaya, Secil Abay, Fuat Aydin, Kadir Semih Gumussoy, Mitat Sahin
In this study, we aimed to report anthrax cases in two pumas, brought to the Pathology Department, Faculty of Veterinary Medicine, Erciyes University for suspected poisoning upon their sudden death at the Kayseri Zoo, in Turkey. In the necropsy, enlargement and malacia were observed in the spleens. The cut surfaces of the spleens were in extreme red-blackish color. Bacillus anthracis was isolated as a pure culture from both samples which belong to dead pumas. B. anthracis isolates had pXO1 and pXO2 plasmids...
October 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30369585/intravenous-human-endothelial-progenitor-cell-administration-into-aged-mice-enhances-embryo-development-and-oocyte-quality-by-reducing-inflammation-endoplasmic-reticulum-stress-and-apoptosis
#13
Geon A Kim, Yeonjae Lee, Hyun Jin Kim, Hyun Ju Oh, Sung Keun Kang, Jeong Chan Ra, Byeong Chun Lee
Stem cell therapy has been proposed to restore the function and structure of injured tissues. In the present study, we investigated the ability of human endothelial progenitor cells (hEPCs) to attenuate ovarian aging and dysfunction. Female ICR mice aged 4 and 6 months were injected with cultured hEPCs. Cultured hEPCs were injected intravenously twice with 5 × 104 cells with a 4 day interval. After pregnant mare serum gonadotropin and human chorionic gonadotropin stimulation, oocytes and ovaries of aged mice were collected, cumulus-free oocytes were activated by SrCl2 and gene expression levels related to inflammation, apoptosis, follicle development and endoplasmic reticulum (ER) stress in ovaries were compared...
October 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30369553/genotypic-and-phenotypic-characterization-of-salmonella-enterica-subsp-enterica-serovar-typhimurium-monophasic-variants-isolated-in-thailand-and-japan
#14
Toshiyuki Murase, Hiroichi Ozaki, Patchara Phuektes, Sunpetch Angkititrakul
Monophasic variants of Salmonella enterica serovar Typhimurium isolated in Thailand and Japan were characterized to elucidate the genetic basis of the monophasic phenotype, genetic relatedness, and antimicrobial resistance. A total of 20 Salmonella isolates agglutinated with anti-O4 and anti-H:i serum and not agglutinated with either anti-H:1 or anti-H:2 serum were identified as monophasic variants of Salmonella serovar Typhimurium because they harbored IS200, specific to this serovar, and lacked the fljB gene...
October 25, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30333379/hematological-and-serum-biochemical-parameters-of-rescued-sunda-pangolins-manis-javanica-in-singapore
#15
Ali Anwar Ahmad, Sofeah Samsuddin, Serena Jocelyn Wai Yin Oh, Pedro Martinez-Perez, Abdullah Rasedee
The Sunda pangolin (Manis javanica) population in Southeast Asia faces threats such as poaching and deforestation. Health assessments of rescued individuals including physical examination and blood work are crucial for clinicians to determine the health status of these animals. The establishments of reference intervals of hematology and serum biochemistry are important for identifying clinical abnormalities. The objectives of our study were to establish blood reference intervals for Sunda pangolins, to determine if there are age and sex related differences in hematology and serum biochemistry, and to compare our results with those of a previous study on confiscated Sunda pangolins in Thailand...
October 18, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30333378/effect-of-post-artificial-insemination-treatment-with-two-different-progesterone-intravaginal-devices-on-conception-and-synchronization-of-the-returning-estrus-in-japanese-black-cows
#16
Naoki Yamamoto, Ryo Nishimura, Yosuke Gunji, Makoto Saneshige, Kensuke Kiriki, Mitsugu Hishinuma
The objective of this study was to evaluate the effects of post artificial insemination (AI) treatment with intravaginal progesterone device (P4 device) on conception rate, synchronization of returning estrus and plasma P4 concentration in Japanese Black cows. Nineteen cows were treated with DIB (1.0 g P4) from Day 12 to 19 (Day 0=day of the first AI), 27 cows were treated with a CIDR (1.9 g P4) from Day 12 to 19, and 33 cows were not treated after the first AI (control). Estrous behavior was daily examined between Day 20 and 25, and cows returning to estrus were inseminated (the second AI)...
October 18, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30333382/follicle-sinus-complexes-fscs-in-muzzle-skin-as-postmortem-diagnostic-material-of-rabid-dogs
#17
Nozomi Shiwa, Chikage Nakajima, Kazunori Kimitsuki, Daria Llenaresas Manalo, Akira Noguchi, Satoshi Inoue, Chun-Ho Park
Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored the virus genome and particles. The viral antigen was detected in 211 of 226 samples with 100% sensitivity and specificity. Viral RNA and particles were observed in the cytoplasm of Merkel cells (MCs). These results suggest that MCs are targets of virus infection and FSCs are useful material for diagnosing rabies...
October 17, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30333381/immunohistochemical-studies-on-meningoencephalitis-in-feline-infectious-peritonitis-fip
#18
Huanan Wang, Miyuki Hirabayashi, James K Chambers, Kazuyuki Uchida, Hiroyuki Nakayama
The present study describes the association between inflammatory cell types and feline infectious peritonitis virus (FIPV) antigen in the brain of 4 cats diagnosed as feline infectious peritonitis (FIP). Immunohistochemically, FIPV antigens were detected in the inflammatory foci of the leptomeninges, choroid plexus and ventricles in 3 of the 4 cats. In 3 cases, inflammatory foci mainly consisted of CD204- and Iba1-positive macrophages, and the FIPV antigens were found in the macrophages. In the other case which was negative for FIPV antigen, severe inflammation predominantly consisting of CD20-positive B lymphocytes was observed in the leptomeninges and subventricles, accompanied with diffuse proliferation of gemistocytic astrocytes...
October 17, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30333360/novel-treatment-for-chronic-pododermatitis-in-an-indian-elephant-elephas-maximus-indicus-with-mohs-paste
#19
Nobuhide Kido, Sohei Tanaka, Tomoko Omiya, Yasuyuki Shoji, Masaru Senzaki, Sayuri Hanzawa, Masato Ando, Tomohiro Osaki, Hitoshi Hatai, Noriaki Miyoshi, Tatsuro Hifumi, Naomi Suzuki, Shigehisa Kawakami
Asian and African elephants are frequently afflicted by foot disorders that can be very challenging to manage even with aggressive therapy. Such conditions may have indirect life-threatening effects. Mohs' paste (zinc chloride based escharotic agent) was used to treat a female Indian elephant (Elephas maximus indicus) aged 39 years with foot disorder at Kanazawa Zoological Gardens. Degenerated hyperplastic tissue was observed inside the hoofs of digits 2 and 5. Mohs' paste was applied on the lesions, which coagulated the hyperplastic tissue and restrained its proliferation...
October 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30305467/elisa-using-a-recombinant-chimera-of-esat-6-mpb70-mpb83-for-mycobacterium-bovis-diagnosis-in-naturally-infected-cattle
#20
Ingrid Ieda Fernando Souza, Rudielle de Arruda Rodrigues, Kláudia Santos Gonçalves Jorge, Márcio Roberto Silva, Walter Lilenbaum, Carlos Eugênio Soto Vidal, Rodrigo Nestor Etges, Miladin Kostovic, Flábio Ribeiro Araújo
Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M...
October 11, 2018: Journal of Veterinary Medical Science
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