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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/28626150/comparison-of-sperm-subpopulation-structures-in-first-and-second-ejaculated-semen-from-japanese-black-bulls-by-a-cluster-analysis-of-sperm-motility-evaluated-by-a-casa-system
#1
Chihiro Kanno, Kentaro Q Sakamoto, Yojiro Yanagawa, Yoshiyuki Takahashi, Seiji Katagiri, Masashi Nagano
In the present study, bull sperm in the first and second ejaculates were divided into subpopulations based on their motility characteristics using a cluster analysis of data from computer-assisted sperm motility analysis (CASA). Semen samples were collected from 4 Japanese black bulls. Data from 9,228 motile sperm were classified into 4 clusters; 1) very rapid and progressively motile sperm, 2) rapid and circularly motile sperm with widely moving heads, 3) moderately motile sperm with heads moving frequently in a short length, and 4) poorly motile sperm...
June 18, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28626159/limbal-xanthogranuloma-in-a-dog
#2
Juri Ota-Kuroki, Keiichi Kuroki
A 10-year-old female spayed American Pitt Bull Terrier was presented with a slow growing mass on the temporal limbus area of the right eye. Canine nodular granulomatous episclerokeratitis was suspected, and the affected eye was treated with 1% prednisolone acetate ophthalmic suspension and 0.03% Tacrolimus ophthalmic solution. As the lesion did not respond to the medical treatments and continued to grow, the mass was excised by lamellar sclerokeratectomy. Microscopically, the mass was composed of granulomatous inflammation with intrahistiocytic lipids and lakes of acicular cholesterol clefts, histopathology findings consistent with xanthogranuloma...
June 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28626158/ducks-as-a-potential-reservoir-for-pasteurella-multocida-infection-detected-using-a-new-romph-based-elisa
#3
Rongchang Liu, Cuiteng Chen, Longfei Cheng, Ronghui Lu, Guanghua Fu, Shaohua Shi, Hongmei Chen, Chunhe Wan, Jiansheng Lin, Qiuling Fu, Yu Huang
Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Chinese ducks. The P. multocida ompH gene was cloned into pET32a, and rOmpH was expressed in Escherichia coli BL21 (DE3)...
June 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28592719/effects-of-toceranib-phosphate-palladia-monotherapy-on-multidrug-resistant-lymphoma-in-dogs
#4
Hiroki Yamazaki, Naoki Miura, Yu-Chang Lai, Masashi Takahashi, Yuko Goto-Koshino, Momoi Yasuyuki, Munekazu Nakaichi, Hajime Tsujimoto, Asuka Setoguchi, Yasuyuki Endo
We examined whether multidrug resistant (MDR) canine lymphoma increases gene expression for platelet-derived growth factor receptor α (PDGFRα), vascular endothelial growth factor receptor 2 (VEGFR2), and c-KIT, and whether toceranib phosphate (TOC) has potential as a treatment for MDR canine lymphoma. The clinical data showed that PDGFRα expression was higher in canine subjects with MDR T-cell lymphoma than in those with untreated T-cell lymphoma, and that c-KIT expression was greater in subjects with T-cell lymphoma than in those with B-cell lymphoma...
June 6, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579597/pharmacokinetics-of-ceftiofur-in-healthy-and-lipopolysaccharide-induced-endotoxemic-newborn-calves-treated-with-single-and-combined-therapy
#5
Feray Altan, Kamil Uney, Ayse Er, Gul Cetin, Burak Dik, Enver Yazar, Muammer Elmas
The aim of this research was to compare plasma pharmacokinetics of ceftiofur sodium (CS) in healthy calves, and in calves with experimentally induced endotoxemia. Six calves received CS (2.2 mg/kg, IM) 2 hr after intravenous administration of 0.9% NaCl (Ceft group). After a washout period, the same 6 calves received CS 2 hr after intravenous injection of lipopolysaccharide (LPS+Ceft group). Another group of 6 calves received a combination of drug therapies that included CS 2 hr after administration of 0.9% NaCl (Comb group)...
June 5, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579596/aldehyde-dehydrogenase-activity-identifies-a-subpopulation-of-canine-adipose-derived-stem-cells-with-higher-differentiation-potential
#6
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Shotaro Etoh, Toshie Iseri, Kenji Tani, Munekazu Nakaichi, Yasuho Taura, Kazuhito Itamoto
Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity-a widely used stem cell marker in mice and humans-by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity...
June 5, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579575/prenatal-and-early-postnatal-noael-dose-clothianidin-exposure-leads-to-a-reduction-of-germ-cells-in-juvenile-male-mice
#7
Shogo Yanai, Tetsushi Hirano, Takuya Omotehara, Tadashi Takada, Naoki Yoneda, Naoto Kubota, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi
Neonicotinoids are pesticides used worldwide. They bind to insect nicotinic acetylcholine receptors (nAChRs) with high affinity. We previously reported that clothianidin (CTD), one of the latest neonicotinoids, reduced antioxidant expression and induced germ cell death in the adult testis of vertebrates. Here, we investigated the male reproductive toxicity of prenatal and early postnatal exposure to CTD, because it is likely that developmental exposure more severely affects the testis compared to adults due to the absence of the blood-testis barrier...
June 3, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579582/steroidogenic-enzyme-expression-in-estrogen-production-in-the-goat-gastrointestinal-gi-tract-and-the-effect-of-castration
#8
Hadi Mohibbi, Mohammad Ibrahim Qasimi, Kentaro Nagaoka, Gen Watanabe
Extragonadal tissues are known to produce estrogens. At these sites, the C19 precursor is important for aromatase expression for the production of estrogen. Aromatase expression is tissue-specific and is controlled by hormones. Recent studies have shown that rat gastric parietal cells expressed aromatase. Our first objective was to investigate steroidogenic enzyme expression in estrogen biosynthesis; the second objective was to investigate which site(s) of the GI tract expressed steroidogenic enzymes; and the third objective was to assess the effects of castration on steroidogenic enzyme expression...
June 2, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579581/a-nasal-osteoma-with-an-acute-course-in-a-japanese-black-heifer
#9
Reiichiro Sato, Yumi Une, Hiroo Madarame, Hyougo Hanami, Eiichi Kanai, Hironobu Murakami, Atsushi Tsukamoto, Takehito Suzuki, Hideharu Ochiai, Masato Kikuchi, Hidekazu Tanaka, Ken Onda
A 14-month-old Japanese Black heifer presented with unilateral epistaxis and mild swelling of the right face. Radiography revealed a mass with increased radiopacity on the right side of the nasal bridge, extending to the left side. Intranasal endoscopy confirmed a large tumor-like structure protruding into the nasal cavity. Following euthanasia, cranial computed tomography (CT) was performed, revealing a tumor 24.3 × 17.5 × 14.8 cm in size. The tumor occupied the entire right nasal cavity and the frontal and sphenoid sinuses...
June 2, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28579580/molecular-prevalence-and-genotyping-of-chlamydia-spp-in-wild-birds-from-south-korea
#10
Jipseol Jeong, Injung An, Jae-Ku Oem, Seung-Jun Wang, Yongkwan Kim, Jeong-Hwa Shin, Chanjin Woo, Youngsik Kim, Seong-Deok Jo, Kidong Son, Saemi Lee, Weonhwa Jheong
Wild birds are reservoirs for Chlamydia spp. Of the total 225 samples from wild birds during January to September 2016 in Korea, 4 (1.8%) and 2 (0.9%) showed positive for Chlamydia psittaci and Chlamydia gallinacea, respectively. Phylogenetic analyses and comparisons of sequence identities for outer-membrane protein A (ompA) revealed that Korean C. psittaci fall into three previously known genotypes; genotype E, 1V and 6N, whereas the Korean C. gallinacea were classified as new variants of C. gallinacea. Our study demonstrates that wild birds in South Korea carry at least two Chlamydia species: C...
June 2, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28552861/effects-of-pentosan-polysulfate-and-polysulfated-glycosaminoglycan-on-chondrogenesis-of-canine-bone-marrow-derived-mesenchymal-stem-cells-in-alginate-and-micromass-culture
#11
Eugene C Bwalya, Sangho Kim, Jing Fang, H M Suranji Wijekoon, Kenji Hosoya, Masahiro Okumura
Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chondrogenic differentiation medium (CDM) was supplemented with PPS or PSGAG at concentrations of 0 (positive control; PC), 1, 3 and 5 μg/ml...
May 27, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28552860/sequence-analysis-of-the-hypervariable-region-in-hmtp210-of-avibacterium-paragallinarum
#12
Edward Araya-Hidalgo, Cristina Gutiérrez-Jiménez, Mónica Chaves-Ramírez, Marcela Suárez-Esquivel, Caterina Guzmán-Verri, Elías Barquero-Calvo
The hmtp210 gene of Avibacterium paragallinarum, the causative agent of infectious coryza,encodes an outer-membrane hemagglutinin (HA) that plays an essential role in pathogenicity. A hypervariable region within this HA, which is highly antigenic, is proposed as a candidate for recombinant vaccine production. Nonetheless, little is known about its genetic variability. We performed sequencing analysis of the hmtp210 hypervariable region in 16 clinical isolates from Costa Rica and compared them with 4 vaccine strains and the hmtp210 sequences available in public databases...
May 27, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28552873/the-therapeutic-efficacy-of-allyl-isothiocyanate-in-cows-with-bovine-digital-dermatitis
#13
Kanako Chiba, Tamako Miyazaki, Yasushi Sekiyama, Masao Miyazaki, Keiji Okada
Bovine digital dermatitis (BDD) is the most prevalent infectious cause of lameness in cattle. Because Treponema infection is a major etiology of BDD, the most common treatment of BDD is an antibiotic. Nonetheless, dairy cows require a withdrawal period after antibiotic treatment before their milk can be marketed. To address the problem, in this study, we tested whether 3 nonantibiotic agents (used separately)-allyl isothiocyanate (AITC), sodium alginate, and calcium hydroxide-alleviate BDD lesions in dairy cows...
May 26, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28539533/evaluation-of-control-measures-for-bovine-viral-diarrhea-implemented-in-nemuro-district-hokkaido-japan-using-a-scenario-tree-model
#14
Norikazu Isoda, Akihiro Asano, Michiru Ichijo, Shiho Wakamori, Hiroshi Ohno, Kazuhiko Sato, Hirokazu Okamoto, Shigeru Nakao, Hajime Kato, Kazuma Saito, Naoki Ito, Akira Usui, Hiroaki Takayama, Yoshihiro Sakoda
A scenario tree model was developed to propose efficient bovine viral diarrhea (BVD) control measures. The model used field data in eastern Hokkaido where the risk of BVDV infection in cattle has been reduced by an eradication program including mass vaccination, individual tests prior to communal pasture grazing, herd screening tests using bulk milk, and outbreak investigations of newly infected herds. These four activities were then used as hypothesized control measures in the simulation. In each simulation, the numbers of cattle infected persistently and transiently with BVDV detected by clinical manifestations and diagnosis tests and of missed by all of the diagnosis tests were calculated, and the numbers were used as indicators to be compared for the efficacy of the control measures...
May 25, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28539532/relationship-between-sociability-toward-humans-and-physiological-stress-in-dogs
#15
Yoon-Joo Shin, Nam-Shik Shin
Sociability is an essential trait for dogs to successfully interact with humans. In this study, the relationship between sociability and physiological stress was examined. Additionally, whether differences exist between companion dogs (C group) and shelter dogs (S group) was examined. Overall, healthy 37 dogs (C group=21 and S group=16) were examined. After 5 min of walking, the dog and the owner (or the chief manager) rested freely in the experimental location for 5 min. The behavioral test with 6 categories was conducted to evaluate sociability over 4 min...
May 25, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529273/septicemic-invasive-klebsiella-pneumoniae-infection-in-a-cynomolgus-monkey-macaca-fascicularis-with-severe-diffused-suppurative-meningoencephalitis
#16
Kazufumi Kasuya, Kou Takayama, Makiko Bito, Natsumi Shimokubo, Ryosuke Kawashima, Tomoyuki Shibahara
A 2-year-old male cynomolgus monkey (Macaca fascicularis) showed neurological symptoms during quarantine for importation into Japan, and was euthanized due to poor prognosis. Gross anatomical examination revealed a hemorrhagic lesion around the lateral ventricle in the cerebrum. Histologically, severe diffused suppurative meningitis and ventriculitis were detected with numerous Gram-negative bacilli in the cerebrum. Immunohistochemically, the bacilli were positively stained with an antibody against Klebsiella pneumoniae...
May 22, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529272/congenital-anterior-staphyloma-associated-with-peters-anomaly-and-aphakia-in-a-holstein-calf
#17
Reiichiro Sato, Ken Onda, Masaru Murakami, Daiya Ito, Hiroo Madarame
A Holstein calf was born with a large protruding right eye and a central corneal opacity. Enucleation was the first choice of treatment. The calf had a good prognosis and was raised for milking purposes. Macroscopically, the enucleated eye was characterized by the protruded cornea, adherence of the iris to the central posterior cornea and aphakia. Microscopically, central corneal thickening and a defect in the endothelium and Descemet's membrane were observed. These data suggest that this represents a case of unilateral anterior segment dysgenesis consistent with congenital corneal staphyloma with Peters' anomaly and aphakia...
May 22, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529271/evidence-of-infection-with-leptospira-interrogans-and-spotted-fever-group-rickettsiae-among-rodents-in-an-urban-area-of-osaka-city-japan
#18
Kenta Shimizu, Rie Isozumi, Kazutoshi Takami, Isao Kimata, Kanae Shiokawa, Kumiko Yoshimatsu, Yoshimi Tsuda, Sanae Nishio, Jiro Arikawa
We examined 33 rodents captured in an urban area of Osaka City, Japan for IgG antibodies against Seoul virus, severe fever with thrombocytopenia syndrome virus, hepatitis E virus, Leptospira interrogans, Yersinia pestis, spotted fever, typhus and scrub typhus group rickettsiae. We found that 3 (9.1%) and 1 (3.0%) of the 33 rodents had antibodies against L. interrogans and spotted fever group rickettsiae, respectively. DNAs of leptospires were detected from 2 of the 3 seropositive rodents, but DNA of rickettsia was not detected...
May 22, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529270/pancreatic-abscess-in-a-cat-due-to-staphylococcus-aureus-infection
#19
Yuki Nemoto, Tomoya Haraguchi, Takako Shimokawa Miyama, Kosuke Kobayashi, Kaori Hama, Yosuke Kurogouchi, Noriyuki Fujiki, Kenji Baba, Masaru Okuda, Takuya Mizuno
A 16-year-old spayed female American Shorthair cat was presented with lethargy, anorexia, and wamble. Physical and blood examination did not reveal any remarkable findings. Abdominal ultrasonography identified the presence of a localized anechoic structure with a thick wall in contact with the small intestine and adjacent to the liver. Ultrasound-guided fine-needle aspiration of the structure revealed fluid containing numerous cocci and neutrophils. Two days after antibiotic treatment, exploratory laparotomy was performed and the content of the structure was removed before multiple lavages...
May 22, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28529244/identification-of-tumor-initiating-cells-derived-from-two-canine-rhabdomyosarcoma-cell-lines
#20
Takuya Evan Kishimoto, Shoko Yashima, Rei Nakahira, Eri Onozawa, Daigo Azakami, Makoto Ujike, Kazuhiko Ochiai, Toshiyuki Ishiwata, Kimimasa Takahashi, Masaki Michishita
Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells...
May 21, 2017: Journal of Veterinary Medical Science
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