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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/28090006/expression-of-apical-junction-complex-proteins-in-colorectal-mucosa-of-miniature-dachshunds-with-inflammatory-colorectal-polyps
#1
Nozomu Yokoyama, Hiroshi Ohta, Yumiko Kagawa, Rommaneeya Leela-Arporn, Angkhana Dermlim, Khoirun Nisa, Tomoya Morita, Tatsuyuki Osuga, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Mitsuyoshi Takiguchi
We examine the expression of tight junction and adherence junction proteins in the colorectal mucosa of miniature dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). Colorectal mucosa samples were endoscopically obtained from 8 MDs with ICRPs and 8 control dogs for immunoblotting. Paraffin-embedded tissues of surgically resected inflamed lesions from another 5 MDs with ICRPs and full-thickness colorectal specimens from 5 healthy beagles were obtained for immunohistochemistry. The expression patterns of claudin-1, -2, -3, -4, -5, -7 and -8, E-cadherin and β-catenin were analyzed in the non-inflamed mucosa and inflamed mucosa of ICRPs and colorectal mucosa of control dogs by immunoblotting...
January 16, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28070089/development-of-a-one-run-real-time-pcr-detection-system-for-pathogens-associated-with-bovine-respiratory-disease-complex
#2
Mai Kishimoto, Shinobu Tsuchiaka, Sayed Samim Rahpaya, Ayako Hasebe, Keiko Otsu, Satoshi Sugimura, Suguru Kobayashi, Natsumi Komatsu, Makoto Nagai, Tsutomu Omatsu, Yuki Naoi, Kaori Sano, Sachiko Okazaki-Terashima, Mami Oba, Yukie Katayama, Reiichiro Sato, Tetsuo Asai, Tetsuya Mizutani
Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them...
January 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28070088/population-and-growth-related-differences-in-helminthic-fauna-of-finless-porpoises-neophocaena-asiaeorientalis-in-five-japanese-populations
#3
Akira Shiozaki, Masao Amano
A survey of helminths and ectoparasites, including epizoits, was conducted in narrow-ridged finless porpoises (Neophocaena asiaeorientalis) from Japanese five populations using dead stranded or incidentally caught animals. In total, 13 helminth species were found (6 nematodes, 4 trematodes, 2 cestodes, and 1 acanthocephalan) in 137 porpoises. A new location record of Stenurus nanjingensis and a new host record of Tetrabothrius sp. were obtained. Eight species of helminth were considered common in the Japanese populations of the finless porpoise: Pharurus sunameri, Pharurus asiaeorientalis, Nasitrema spathulatum, Nasitrema sunameri, Halocercus pingi, Halocercus sunameri, Campula oblonga, and Synthesium elongatum...
January 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28070087/postnatal-changes-in-the-relative-abundance-of-intestinal-lactobacillus-spp-in-newborn-calves
#4
Tadashi Takino, Yuko Kato-Mori, Daisuke Motooka, Shota Nakamura, Tetsuya Iida, Katsuro Hagiwara
The intestinal microbiota of newborn calves changes during the early postnatal period and influences their health and immune function. We studied the compositional changes in the intestinal microbiome of newborn calves during the first week after birth by metagenomic analysis. In feces from newborn calves, we identified 4 bacterial phyla, namely, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The relative abundance of Lactobacillaceae significantly increased from day 1 to day 7. We evaluated Lactobacillus spp...
January 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28070061/injectable-alginate-microencapsulated-canine-adipose-tissue-derived-mesenchymal-stem-cells-for-enhanced-viable-cell-retention
#5
Eunji Koh, Yun Chan Jung, Heung-Myong Woo, Byung-Jae Kang
The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro...
January 6, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049867/elephant-s-breast-milk-contains-large-amounts-of-glucosamine
#6
Zenta Takatsu, Muneya Tsuda, Akio Yamada, Hiroshi Matsumoto, Akira Takai, Yasuhiro Takeda, Mitsunori Takase
Hand-reared elephant calves that are nursed with milk substitutes sometimes suffer bone fractures, probably due to problems associated with nutrition, exercise, sunshine levels and/or genetic factors. As we were expecting the birth of an Asian elephant (Elephas maximus), we analyzed elephant's breast milk to improve the milk substitutes for elephant calves. Although there were few nutritional differences between conventional substitutes and elephant's breast milk, we found a large unknown peak in the breast milk during high-performance liquid chromatography-based amino acid analysis and determined that it was glucosamine (GlcN) using liquid chromatography/mass spectrometry...
December 31, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049866/genetic-diversity-of-the-yonaguni-horse-based-on-polymorphisms-in-microsatellites-and-mitochondrial-dna
#7
Natsuko Senju, Teruaki Tozaki, Hironaga Kakoi, Akihisa Shinjo, Ryota Matsuyama, Julio Almunia, Masaki Takasu
Thirty-two microsatellites and a mitochondrial DNA haplotypes of endangered Yonaguni horses were analyzed to establish a pedigree registration system and to understand their genetic diversity for planning effective conservation. Blood samples were collected from 78 of the 130 horses in existence, and DNA was extracted and genotyped. There were two major findings. One is that it is possible to use microsatellites for Yonaguni horse pedigree registration in the future because the power of exclusion of parentage testing is reliable at 0...
December 31, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049922/comparison-of-fecal-microbiota-of-three-captive-carnivore-species-inhabiting-korea
#8
Choa An, Yumiko Okamoto, Siyu Xu, Kyung Yeon Eo, Junpei Kimura, Naomichi Yamamoto
This study aimed at characterizing fecal microbiota of three captive carnivore species of leopard cats Prionailurus bengalensis, Eurasian otters Lutra lutra and raccoon dogs Nyctereutes procyonoides. We used DNA barcoding sequencing to analyze 16S rRNA genes of uncultured bacteria in the feces collected in the Seoul Zoo. The sequencing analyses revealed that: 1) Firmicutes was the most dominant phylum for all three animals; 2) bacterial genus-rank compositions were distinct across species of the animals; and 3) bacterial community memberships were different across species of the studied animals...
December 30, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049921/in-vitro-canine-platelet-aggregation-caused-by-dirofilaria-immitis-extract
#9
Yasuhiro Takashima, Isako Onoda, Shin-Pin Chiou, Katsuya Kitoh
Platelet function hyper-activity has been reported in Dirofilaria immitis (heartworm, HW)-infected dogs. Although the mechanism of increased platelet hyper-activity has not yet been elucidated, it is suggested to be mediated by unknown factors, which may be related to adult HW components. This study aims to determine whether adult male HW whole body extract induces canine platelet aggregation in vitro. The results indicate that HW extract caused an aggregation of canine platelets in a concentration-dependent manner...
December 30, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28049868/expression-of-epithelial-cell-derived-cytokine-genes-in-the-duodenal-and-colonic-mucosae-of-dogs-with-chronic-enteropathy
#10
Hironari Osada, Misato Ogawa, Ayana Hasegawa, Makoto Nagai, Junsuke Shirai, Kazuaki Sasaki, Minoru Shimoda, Hiroshi Itoh, Hirotaka Kondo, Keitaro Ohmori
It remains unclear whether epithelial cell-derived cytokines, including interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP), contribute to development of canine chronic enteropathy (CE), which includes antibiotic-responsive enteropathy (ARE), food-responsive enteropathy (FRE) and inflammatory bowel disease (IBD). In the present study, we examined mRNA expression of il-25, il-33 and tslp in the duodenal and colonic mucosae of dogs with ARE, FRE and IBD. Real-time PCR analysis revealed that mRNA expression of il-33 was significantly lower in the duodenum in dogs with FRE than in healthy dogs...
December 29, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28025458/adverse-effects-of-maternal-exposure-to-bisphenol-f-on-the-anxiety-and-depression-like-behavior-of-offspring
#11
Naoko Ohtani, Hidetomo Iwano, Koshi Suda, Erika Tsuji, Kentaro Tanemura, Hiroki Inoue, Hiroshi Yokota
Bisphenol A (BPA), a well-known endocrine disruptor, is metabolized and eliminated rapidly from the body in adult animals. However, many authors have reported that perinatal BPA exposure alters development of the brain, reproductive system and behavior in the next generation. Recently, BPA substitutes, especially bisphenol F (BPF), have been used because of concerns about the influence of BPA on children, although the actual effects on the next generation are unknown. In this study, we observed behavioral adverse effects of the offspring of mice exposed to BPA or BPF in fetal period...
December 25, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28025450/application-of-fiber-tractography-and-diffusion-tensor-imaging-to-evaluate-spinal-cord-diseases-in-dogs
#12
Yuko Konishi, Hiroyuki Satoh, Yasuyoshi Kuroiwa, Mizuki Kusaka, Atsushi Yamashita, Yujiro Asada, Taketoshi Asanuma
Fiber tractography is a technique capable of depicting the three-dimensional structure and connectivity of nerve fibers using serial magnetic resonance diffusion tensor imaging (DTI). To establish fiber tractography and DTI methods in veterinary clinical medicine, we evaluated fiber tractography and DTI parameters: apparent diffusion coefficient (ADC) values and fractional anisotropy (FA) values, in various spinal cord diseases. Spinal cord DTI was examined in 28 dogs with spinal cord diseases. The ADC and FA values were measured at lesion sites and cranial normal sites on spinal cords, and both values of lesion sites were compared with normal sites...
December 23, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27990011/retrospective-analysis-of-canine-gallbladder-contents-in-biliary-sludge-and-gallbladder-mucoceles
#13
Shinya Mizutani, Shidow Torisu, Yasuyuki Kaneko, Shushi Yamamoto, Shinsuke Fujimoto, Benedict Ong Huai Ern, Kiyokazu Naganobu
The pathophysiology of canine gallbladder diseases, including biliary sludge, gallbladder mucoceles, and gallstones, is poorly understood. This study aimed to evaluate the composition of gallbladder contents and bacterial infection of the gallbladder in order to elucidate the pathophysiology of biliary sludge and gallbladder mucoceles. A total of 43 samples of canine gallbladder contents (biliary sludge, 21 and gallbladder mucoceles, 22) were subjected to component analysis by infrared spectroscopy, and the resultant infrared spectra were compared with that of swine mucin...
December 17, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27990010/a-case-of-endoscopic-retrieval-of-a-long-bamboo-stick-from-a-humboldt-penguin-spheniscus-humboldti
#14
Woo-Sung Jung, Minho Ko, Hyun Kee Cho, Byung-Jae Kang, Jung Hoon Choi, Jin-Young Chung
An eighteen-month-old female Humboldt penguin (Spheniscus humboldti) that was 50 cm in length and 4.5 kg in weight was presented with anorexia and vomiting. The hematological and blood biochemical profiles revealed no remarkable findings, and no Salmonella, Shigella or Vibrio spp. were isolated from the fecal culture. However, radiographic imaging revealed a long linear foreign body presenting from the lower esophagus to the stomach. To retrieve this foreign body, flexible endoscopic extraction was performed using flexible rat tooth grasping forceps...
December 17, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27990009/distribution-of-methicillin-resistant-coagulase-positive-staphylococci-mrcops-in-a-surgical-unit-and-cystotomy-operation-sites-in-a-veterinary-teaching-hospital
#15
Fungwithaya Punpichaya, Brikshavana Pasakorn, Chanchaithong Pattrarat, Prapasarakul Nuvee
This study aimed to investigate the spread of methicillin-resistant coagulase-positive staphylococci (MRCoPS) among veterinary staff, hand-touch sites and surgical tissue during cystotomy operations on cats and dogs that were patients, and to analyze the genetic relatedness and antimicrobial resistance profiles of the isolates. Human and environmental samples were obtained from the nasal passageways of 12 surgeons and veterinary assistants and from 29 hand-touch sites of instruments in operative units and subjected to bacterial isolation and enumeration...
December 17, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27980234/effects-of-postural-change-on-transesophageal-echocardiography-views-and-parameters-in-healthy-dogs
#16
Seijirow Goya, Tomoki Wada, Kazumi Shimada, Daiki Hirao, Ryuji Fukushima, Norio Yamagishi, Miki Shimizu, Ryou Tanaka
The purpose of the present study is to investigate the effect of postural change on transesophageal echocardiography (TEE) views and parameters of interest anesthesia monitoring in healthy dogs. Twelve Beagle dogs were anesthetized and randomly positioned in one of four postures: right lateral-recumbency, left lateral-recumbency, supine position and prone position. After examinations in one posture, the same examination was demonstrated in another posture and repeated in all postures. In each posture, several standard TEE views were demonstrated: longitudinal cranial-esophageal aorta long-axis-view, transverse middle-esophageal mitral valve long-axis-view and transgastric middle short-axis-view...
December 16, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27980252/experimental-induction-of-necrotic-enteritis-in-chickens-by-a-netb-positive-japanese-isolate-of-clostridium-perfringens
#17
Ho To, Takayuki Suzuki, Fumiya Kawahara, Koji Uetsuka, Shinya Nagai, Tetsuo Nunoya
Necrotic enteritis (NE) is one of the most important bacterial diseases in terms of economic losses. Clostridium perfringens necrotic enteritis toxin B, NetB, was recently proposed as a new key virulent factor for the development of NE. The goal of this work was to develop a necrotic enteritis model in chickens by using a Japanese isolate of C. perfringens. The Japanese isolate has been found to contain netB gene, which had the same nucleotide and deduced amino acid sequences as those of prototype gene characterized in Australian strain EHE-NE18, and also expressed in vitro a 33-kDa protein identified as NetB toxin by nano-scale liquid chromatographic tandem mass spectrometry...
December 15, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27980290/segmental-spinal-dysgenesis-with-caudal-agenesis-in-a-holstein-calf
#18
Kaori Tokudome, Shuichi Ito, Tomoko Kato, Tohru Yanase, Tatsuo Kawarasaki, Yasuo Moritomo
A rare complex dysraphic malformation, comprising segmental spinal dysgenesis with caudal agenesis, was found in a Holstein calf that was unable to stand and was slightly short at the lumbosacral spine with taillessness. The thoracolumbar and sacrococcygeal regions of the midline axial segments showed severe deformities. In the spinal cord, the thoracolumbar region showed severe constriction with myelodysplastic changes, and the sacrococcygeal region showed dorsoventral separation with connection to a neural mass...
December 13, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27941298/degenerative-myelopathy-in-the-collie-breed-a-retrospective-immunohistochemical-analysis-of-superoxide-dismutase-1-in-an-affected-rough-collie-and-a-molecular-epidemiological-survey-of-the-sod1-c-118g-a-mutation-in-japan
#19
Moeko Kohyama, Masato Kitagawa, Hiroaki Kamishina, Yui Kobatake, Akira Yabuki, Mariko Sawa, Shusaku Kakita, Osamu Yamato
Canine degenerative myelopathy (DM) is an adult-onset, progressive neurodegenerative disease that occurs in multiple dog breeds. A DM-associated mutation of the canine superoxide dismutase 1 (SOD1) gene, designated as c.118G>A (p.E40K), has been implicated as one of pathogenetic determinants of the disease in many breeds, but it remains to be determined whether the c.118G>A mutation is responsible for development or progression of DM in Collies. Previously, a Rough Collie was diagnosed clinically and histopathologically as having DM in Japan, suggesting the possibility that the Collie breed may be predisposed to DM due to the high frequency of c...
December 12, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27941297/serum-levels-of-ochratoxin-a-in-dogs-with-chronic-kidney-disease-ckd-a-retrospective-study
#20
Meucci Valentina, Luci Giacomo, Vanni Michele, Guidi Grazia, Perondi Francesca, Intorre Luigi
Ochratoxin A (OTA) is a mycotoxin produced by secondary metabolism of several fungi belonging to the genera Aspergillus and Penicillium. OTA is potentially nephrotoxic, neurotoxic, immunotoxic and carcinogenic in several animal species and in humans. This toxin has been detected in several human food and animal feed. The aim of this study was to determine OTA in blood samples of healthy and affected by chronic kidney disease (CKD) dogs. CKD group showed higher incidence of OTA-positivity than healthy dogs (96% vs...
December 12, 2016: Journal of Veterinary Medical Science
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