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Journal of Veterinary Medical Science

Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Shiro Murata, Kazuhiko Ohashi
Sheep-associated malignant catarrhal fever (SA-MCF), caused by ovine gammaherpesvirus-2 (OvHV-2), is a fatal disease in all ruminants. The epidemiological survey and molecular characterization of OvHV-2 in Mongolian livestock were performed. Of 928 blood samples, 14 were positive for OvHV-2 in sheep and native cattle from Tsenkher County and in sheep from Lun County. Phylogenetic analyses revealed that the tegument gene of OvHV-2 sequences from Mongolian animals is identical to that in animals from Egypt, India, and Turkey, and is 98...
October 19, 2017: Journal of Veterinary Medical Science
Cuong Van Dao, Mitsuya Shiraishi, Atsushi Miyamoto
Methylmercury (MeHg) is an environmental pollutant that shows severe toxicity to humans and animals. However, the molecular mechanisms mediating MeHg toxicity are not completely understood. We have previously reported that the MARCKS protein is involved in the MeHg toxicity to SH-SY5Y neuroblastoma and EA.hy926 vascular endothelial cell lines. In addition, calpain, a Ca(2+)-dependent protease, is suggested to be associated with the MeHg toxicity. Because MARCKS is known as a substrate of calpain, we studied the relation between calpain activation and cleavage of MARCKS and its role in MeHg toxicity...
October 18, 2017: Journal of Veterinary Medical Science
Toshihiko Matsuo, Tetsuya Uchida, Makoto Nitta, Koichiro Yamashita, Shigiko Takei, Daisuke Ido, Mamoru Tanaka, Masao Oguchi, Toshinori Furukawa
Okayama University-type retinal prosthesis (OUReP(TM)) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery...
October 18, 2017: Journal of Veterinary Medical Science
Mei-Qing Huang, Xiao-Xia Cheng, Shi-Long Chen, Min Zheng, Shao-Ying Chen
Muscovy duck reovirus (MDRV) belongs to the Orthoreovirus genus of the Reoviridae family, which is a significant poultry pathogen leading to high morbidity and mortality in ducklings. However, the pathogenesis of the virus is not well understood. In the present study, two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) combined with LC-MS-MS was used to identify differentially expressed proteins between Muscovy duck embryo fibroblasts (MDEF) infected with virulent (MV9710 strain) and attenuated (CA strain) MDRV and non-infected MDEFs...
October 18, 2017: Journal of Veterinary Medical Science
Ai Hashimoto, Mohammad Rabiul Karim, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate
Pancreatic fibrosis develops as the results of the activity of myofibroblasts capable of producing collagens. The myofibroblasts derive from pancreatic interstitial cells, including pancreatic stellate cells (PSCs), which can express glial fibrillary acidic protein (GFAP). First, we investigated the expression patterns of vimentin, desmin, α-smooth muscle actin (α-SMA), Thy-1, and GFAP in the developing rat pancreas (in fetuses at 18 and 20 days, neonates from 1 to 21 days, and adults). Interstitial cells in the developing pancreas expressed vimentin, desmin, GFAP, and Thy-1 at varying degrees; interestingly, the reactivity for desmin and vimentin was the highest in fetuses...
October 17, 2017: Journal of Veterinary Medical Science
Ryo Nishimura, Hiroki Hasegawa, Masamichi Yamashita, Norihiko Ito, Yoshiharu Okamoto, Takashi Takeuchi, Tomoaki Kubo, Kosuke Iga, Koji Kimura, Mitsugu Hishinuma, Kiyoshi Okuda
A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells...
October 17, 2017: Journal of Veterinary Medical Science
Megumi Itoh, Takahiro Aoki, Yoshie Sakurai, Naoki Sasaki, Hisashi Inokuma, Satoshi Kawamoto, Kazutaka Yamada
To clarify the mechanisms underlying the development of displaced abomasum (DA), we repositioned DA in 12 cows by rolling and observed the course of redisplacement using X-ray fluoroscopy. Displacement of the abomasum was observed to follow three patterns: displacement to the left side of the abdominal cavity through the gap formed at the reticulum, atrium of the rumen and ventral sac of the rumen (n=7); displacement to the left side after cranial movement along the diaphragm (n=3); displacement to the right side after cranial movement along the diaphragm (n=2)...
October 17, 2017: Journal of Veterinary Medical Science
Reiichiro Sato, Eiichi Kanai, Go Kitahara, Michiko Noguchi, Kazuhiro Kawai, Yasunori Shinozuka, Atsushi Tsukamoto, Hideharu Ochiai, Ken Onda, Adrian Steiner
The main objective of this study was to evaluate the effects of transrectal guidance of the ovaries by an assistant on operative time during bovine laparoscopic ovariectomy. Twenty four clinically healthy Holstein dairy cows were divided randomly into two groups. In the transrectal guidance group, an assistant grasped the ovaries via the transrectal route and pulled them to a position where they could be visualized with a camera. On the other hand, the control group was operated without guidance. The time required to remove both ovaries in the guidance group was shorter than that in the control group (P<0...
October 13, 2017: Journal of Veterinary Medical Science
Shin-Ichiro Fukumoto, Seitaro Yamada, Manato Fushikida, Shotaro Toyada, Tomotaka Nishikawa, Hidetoshi Higuchi, Hiroshi Ueno, Hiromi Ueda, Hiromu Sugiyama, Yasuyuki Morishima
Natural infection with larval Echinococcus multilocularis was recognized in one of eight Norway rats, Rattus norvegicus, caught indoors in 2009 in Ebetsu, Hokkaido, northern Japan. Cystic lesions were found in the right median and lateral lobes of the liver, with numerous alveolar cysts in the periphery of the lesions. Protoscolices were formed within large cysts. The laminated layers of the cysts were positive for PAS staining. Nested PCR using the primers specific for Taenia mitochondrial 12S rDNA yielded a 250-bp product, and the sequence of the PCR product matched that of E...
October 11, 2017: Journal of Veterinary Medical Science
Aki Fujiwara-Igarashi, Koichi Shimizu, Masaki Michishita, Yoshihiko Yu, Yuji Hamamoto, Daisuke Hasegawa, Michio Fujita
A 16-year-old castrated male mongrel cat presented with swelling under the left pinna and a 3 -month history of voice change. Laryngeal endoscopy revealed circumferential oedema around the arytenoid cartilages and hypersecretion of saliva. Histopathological examination of the mass around the left ear canal was considered the primary lesion that originated from cutaneous apocrine adenocarcinoma or parotid gland adenocarcinoma, and it metastasized to the larynx, lung and medial retropharyngeal lymph nodes. This report provides new insights into feline laryngeal diseases which could result in laryngeal metastasis with slight mucosal irregularity alone and without obvious radiographic abnormalities...
October 11, 2017: Journal of Veterinary Medical Science
Tumenjargal Sharav, Satoru Konnai, Nyamsuren Ochirkhuu, Erdene Ochir Ts, Hirohisa Mekata, Yoshihiro Sakoda, Takashi Umemura, Shiro Murata, Tungalag Chultemdorj, Kazuhiko Ohashi
The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94...
October 11, 2017: Journal of Veterinary Medical Science
Miyuu Tanaka, Shinobu Yamaguchi, Hideo Akiyoshi, Masaya Tsuboi, Kazuyuki Uchida, Takeshi Izawa, Jyoji Yamate, Mitsuru Kuwamura
Neuroaxonal dystrophy (NAD) is a neurodegenerative disease characterized by severe axonal swelling (spheroids) throughout the nervous system. In dogs, NAD has been reported in several breeds and a missense mutation in PLA2G6 gene has recently been identified in the Papillon dog NAD. Here we performed ultrastructural analysis to clarify the detailed ultrastructural features of the Papillon dog NAD. Dystrophic axons consisted of accumulation of filamentous materials, tubulovesicular structures, and swollen edematous mitochondria with degenerated inner membranes were often observed in the CNS...
October 10, 2017: Journal of Veterinary Medical Science
Hideto Yuasa, Youhei Mantani, Natsumi Masuda, Miho Nishida, Masaya Arai, Toshifumi Yokoyama, Hiroki Tsuruta, Junichi Kawano, Nobuhiko Hoshi, Hiroshi Kitagawa
The mechanism by which indigenous bacteria on the follicle-associated epithelium (FAE) of lymphatic follicles (LFs) accelerate the differentiation of microvillous columnar epithelial cells (MV) into M-cells was immunohistochemically investigated in rat Peyer's patches. The results showed that the number of Toll-like receptor (TLR) -4(+) M-cells was greater in the FAE with expansion of bacterial colonies (LFs with bacterial colonies on the FAE: b-LF) than the FAE without expansion of bacterial colonies (nb-LF)...
October 8, 2017: Journal of Veterinary Medical Science
Jianbao Dong, Wei Zhu, Nanako Yamashita, James K Chambers, Kazuyuki Uchida, Atsutoshi Kuwano, Takeshi Haga
Although many studies have been conducted worldwide for Equus caballus papillomavirus (EcPV), limited information is available on the virus in Japan. We recently collected one classical viral papillomatosis sample (E150904) from a racing horse in Japan. Papillomavirus infection was confirmed by histopathology, immunohistochemistry and PCR assays, and the sample was diagnosed as epithelial papilloma. Full-length genome of the virus was cloned and sequenced. It was 7,613 bp in length and had the same genome organization with reported EcPV-1...
October 8, 2017: Journal of Veterinary Medical Science
Kenji Kutara, Toshiaki Konno, Hirotaka Kondo, Hinako Yamazoe, Satoru Matsunaga
A 10-year-old French bulldog presented with an abdominal tumor. Triple-phase helical computed tomography was performed, revealing a hepatic tumor, an enlarged hepatic lymph node, and no masses in other organs. The hepatic tumor demonstrated marked enhancement, similar to that of the aorta in the arterial phase. The tumor had rich vascularization and a hepatic arterio-venous shunt formed between the hepatic artery and middle hepatic vein. The hepatic tumor was surgically removed and histological diagnosis revealed a hepatic carcinoid tumor...
October 7, 2017: Journal of Veterinary Medical Science
Mizuho Suzuki, Masatoshi Okamatsu, Takahiro Hiono, Keita Matsuno, Yoshihiro Sakoda
H2N2 influenza virus caused a pandemic starting in 1957 but has not been detected in humans since 1968. Thus, most people are immunologically naive to viruses of the H2 subtype. In contrast, H2 influenza viruses are continually isolated from wild birds, and H2N3 viruses were isolated from pigs in 2006. H2 influenza viruses could cause a pandemic if re-introduced into humans. In the present study, a vaccine against H2 influenza was prepared as an effective control measure against a future human pandemic. A/duck/Hokkaido/162/2013 (H2N1), which showed broad antigenic cross-reactivity, was selected from the candidate H2 influenza viruses recently isolated from wild birds in Asian countries...
October 7, 2017: Journal of Veterinary Medical Science
Kenji Kutara, Mamiko Seki, Kumiko Ishigaki, Kenji Teshima, Chieko Ishikawa, Yumiko Kagawa, Kazuya Edamura, Tomohiro Nakayama, Kazushi Asano
We investigated the utility of triple-phase helical computed tomography (CT) in differentiating between benign and malignant splenic masses in dogs. Forty-two dogs with primary splenic masses underwent triple-phase helical CT scanning (before administration of contrast, and in the arterial phase, portal venous phase, and delayed phase) prior to splenectomy. Tissue specimens were sent for pathological diagnosis; these included hematomas (n=14), nodular hyperplasias (n=12), hemangiosarcomas (n=11), and undifferentiated sarcomas (n=5)...
October 7, 2017: Journal of Veterinary Medical Science
Etsuko Katsumata, Sukanya Jaroenporn, Yoko Ueda, Kazutoshi Arai, Hiroshi Katsumata, Gen Watanabe, Kazuyoshi Taya
To reveal the reproductive biology in male bottlenose dolphins (Tursiops truncatus), circulating gonadotropins (follicle stimulating hormone [FSH] and luteinizing hormone [LH]) and testicular hormones (testosterone and inhibin) were monitored for 8 -12 years in 2 captive bottlenose dolphins (Mars and Regulus). During the study period, Mars was undergoing sexual maturation, whereas Regulus was already mature at the beginning of the study. Assuming that Mars had reached sexual maturity when the significant increase in circulating testosterone levels was observed, serum concentration of inhibin was higher in the sexually immature stage than in the mature stage, whereas the serum concentration of FSH was higher in the sexually mature stage than in the immature stage...
October 7, 2017: Journal of Veterinary Medical Science
Naomi Terada, Mohammad Rabiul Karim, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate
β-catenin, E-cadherin and N-cadherin are adhesion molecules that play important roles in organogenesis, tissue homeostasis, renal epithelial integrity and polarity. The present study demonstrated their immunolocalization in adult and neonate rat kidney. Membranous or cytoplasmic expression of β-catenin, E-cadherin and N-cadherin were seen in adult and developing renal tubular epithelial cells. Particularly, in adult kidney, E-cadherin and β-catenin were intensively expressed in distal renal tubules, whereas N-cadherin was expressed in proximal renal tubules...
October 6, 2017: Journal of Veterinary Medical Science
Manabu Nemoto, Toru Kanno, Hiroshi Bannai, Koji Tsujimura, Takashi Yamanaka, Hiroshi Kokado
A vaccine for equine coronavirus (ECoV) is so far unavailable. Bovine coronavirus (BCoV) is antigenically related to ECoV; it is therefore possible that BCoV vaccine will induce antibodies against ECoV in horses. This study investigated antibody response to ECoV in horses inoculated with BCoV vaccine. Virus neutralization tests showed that antibody titers against ECoV increased in all six horses tested at 14 days post inoculation, although the antibody titers were lower against ECoV than against BCoV. This study showed that BCoV vaccine provides horses with antibodies against ECoV to some extent...
October 6, 2017: Journal of Veterinary Medical Science
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