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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/30232303/short-term-outcome-of-laparoscopic-cholecystectomy-for-benign-gall-bladder-diseases-in-76-dogs
#1
Hiroo Kanai, Ken Hagiwara, Aya Nukaya, Motoki Kondo, Akihide Aso
Laparoscopic cholecystectomy (LC) is widely accepted as the standard treatment for benign gall bladder diseases in humans because it has proven to be less invasive and safer than are traditional methods. However, the efficacy of LC in dogs remains unclear. The present study aimed to examine the short-term outcome of LC for benign gall bladder diseases in dogs. We enrolled 76 consecutive dogs that underwent LC for benign gall bladder diseases at our hospital between April 2008 and October 2016. Dogs with jaundice, gall bladder ruptures, abdominal effusion, or extrahepatic biliary obstruction were not excluded from the indication...
September 20, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30232302/combined-cutting-balloon-and-conventional-balloon-angioplasty-in-a-dog-with-supravalvular-pulmonary-stenosis
#2
Seijirow Goya, Tomoki Wada, Kazumi Shimada, Akiko Uemura, Ryou Tanaka
A 7-year-old Miniature Schnauzer presented with exercise intolerance and easy fatigability. Echocardiography revealed the presence of supravalvular pulmonary stenosis. The peak velocity through the stenosis was 6.4 m/sec, and the interventricular septum was flattened. Cutting balloon angioplasty was designed for the treatment of coronary artery stenosis, which was resistant to conventional balloon angioplasty. Accordingly, the dog underwent cutting balloon angioplasty and conventional balloon dilation. One month after treatment, it showed neither exercise intolerance nor easy fatigability...
September 20, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30232316/canine-spindle-cell-tumor-mimicking-human-classical-hemangiopericytoma
#3
Takashi Suzuki, Narumi Yamamoto, Hirotaka Kondo, Hisashi Shibuya
The neoplastic mass developed in the left flank of a Border Collie dog. The tumor was resected surgically and evaluated histologically and immunohistochemically. Histologically a moderate number of spindle cells were proliferated with staghorn, placentoid, and myxoid growth patterns and a lack of perivascular whirling. Immunohistochemically, the tumor cells were positive to vimentin, laminin, S-100 protein, CD34 and CD117 antibodies. They were negative to cytokeratin AE1/3, desmin, α-SMA and calponin antibodies...
September 19, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30224573/identification-of-novel-haplotypes-and-interpretation-of-gene-flow-of-mitochondrial-dna-control-region-of-eurasian-otter-lutra-lutra-for-the-effective-conservation
#4
Ji-Yong Hwang, Gil-Jae Cho
The number and distribution of Eurasian otters have declined during twentieth century due to human activity and water pollution. The global conservation status of Eurasian otter is presently 'Near Threatened (NT)' and strictly protected by being listed on the international legislation and conventions. A number of studies using the mitochondrial DNA (mtDNA) control region (CR) have been conducted in order to effectively apply conservation and reintroduction programs, especially in Europe. However, aside from Europe, there have been few studies concerning genetic diversity and phylogeny of Eurasian otters...
September 17, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30224577/pathogenesis-of-the-attenuated-foot-and-mouth-disease-virus-o-jpn-2000-in-experimentally-infected-pigs
#5
Manabu Yamada, Katsuhiko Fukai, Kazuki Morioka, Tatsuya Nishi, Reiko Yamazoe, Rie Kitano, Nobuaki Shimada, Kazuo Yoshida, Toru Kanno, Kenichi Sakamoto, Makoto Yamakawa
We examined the pathogenesis of the attenuated foot-and-mouth disease virus (FMDV) O/JPN/2000 in pigs. The virus used in this study was passaged three times in primary bovine kidney (BK) cells and once in baby hamster kidney-21 (BHK-21) cells after isolation. A plaque assay demonstrated that this virus exhibited the small plaque (SP) phenotype. There was no clinical or histological evidence of vesicular lesions in pigs intraorally inoculated with 106 50% tissue culture infectious dose (TCID50 )/ml of the SP virus (SPV) of FMDV O/JPN/2000...
September 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30224576/actinomyces-denticolens-as-a-causative-agent-of-actinomycosis-in-animals
#6
Satoshi Murakami, Tomoko Kobayashi, Yuriko Sekigawa, Yasushi Torii, Yu Kanesaki, Taichiro Ishige, Eiji Yokoyama, Hiroyuki Ishiwata, Moriyuki Hamada, Tomohiko Tamura
The name "Actinomyces suis" was applied to each actinomycete isolate from swine actinomycosis by Grässer in 1962 and Franke in 1973. Nevertheless, this specific species was not included in the "Approved List of Bacterial Name" due to absence of the type cultures. Therefore, "Actinomyces suis" based on the description of Franke 1973 has been considered as "species incertae sedis". We isolated a number of Actinomyces strains from swine. The representative strains of them was designated as Chiba 101 that was closely similar to the description in "Actinomyces suis" reported by Franke in 1973...
September 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30224575/assessment-of-fish-iridoviruses-using-a-novel-cell-line-gs-1-derived-from-the-spleen-of-orange-spotted-grouper-epinephelus-coioides-hamilton-and-susceptible-to-ranavirus-and-megalocytivirus
#7
Sue-Min Huang, Shu-Ting Kuo, Hung-Chih Kuo, Shao-Kuang Chang
A new cell line (GS-1) was developed from the spleen tissue of the orange-spotted grouper, Epinephelus coioides applied for viral infection studies of fish ranavirus and megalocytivirus. The cells proficiently multiplied in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at temperatures between 20°C and 32°C. Morphologically, the cell line comprised fibroblast-like cells, and this was confirmed by immunostaining with vimentin, fibronectin, and desmin antibodies. The optimal temperature for GIV and ISKNV proliferation in GS-1 cells was 25°C, and the highest titer of grouper iridovirus (GIV) was 108...
September 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210072/stability-of-selected-enzymes-in-saliva-of-pigs-under-different-storage-conditions-a-pilot-study
#8
Damián Escribano, María D Contreras-Aguilar, Asta Tvarijonaviciute, Silvia Martínez-Miró, Silvia Martínez-Subiela, José J Cerón, Elsa Lamy, Fernando Tecles
Stability of α-amylase (α-A), butyrylcholinesterase (BChE), lipase, adenosine deaminase (ADA) and total esterase activity (TEA) in two pools of porcine saliva was studied after 1 and 4 days at 4º C, and after 30, 90 and 360 days at -20º and -80ºC. At 4ºC, BChE, lipase and TEA were stable less than 1 day, α-A less than 4 days and ADA for up to 4 days. At -20º C, BChE and TEA were stable less than 30 days, α-A and lipase less than 90 days and ADA up to 360 days. At -80º C, TEA was stable less than 30 days, α-A and lipase less than 360 days, and BChE and ADA for up to 360 days...
September 13, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210095/bovine-vegetative-endocarditis-caused-by-streptococcus-suis
#9
Tetsuya Komatsu, Eri Watando, Nanami Inaba, Kennosuke Sugie, Masatoshi Okura, Tomoyuki Shibahara
A 5-month-old crossbred beef steer died after exhibiting astasia. A postmortem examination revealed verrucous endocarditis and numerous renal hemorrhages. Gram-positive bacteria were identified in the necrotic lesions of the verruca and mitral valve via histopathological analysis. Multifocal necrosis and hemorrhage were detected in the renal cortex. Gram-positive cocci isolated from the verruca were identified via biochemical tests and 16S rRNA gene sequence analysis as Streptococcus suis. Serotyping indicated that the S...
September 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210094/full-genome-analysis-of-bovine-papillomavirus-type-1-derived-from-a-calf-with-severe-cutaneous-multiple-papillomatosis
#10
Hidekatsu Shimakura, Jianbao Dong, Wei Zhu, James K Chambers, Kazuyuki Uchida, Kensuke Kiriki, Mizuho Uematsu, Yoshitaka Goto, Masahiro Yasuda, Nanako Yamashita, Masano Tsuzuki, Dung T LE, Shinichi Hatama, Takeshi Haga
Severe papillomatosis occasionally causes astasia leading to euthanizing cattle. There are currently a limited number of reports on virologic approach in severe bovine papillomatosis. Here we report a full genome characterization of bovine papillomavirus type 1 (BPV-1) from the case of severe papillomatosis. A calf developed numerous papillomas on the skin and some nodules in the upper gastrointestinal tract at seven months old. The skin lesion was diagnosed as the epithelial papilloma with BPV antigen expression, while the gastrointestinal lesions were diagnosed as the fibropapilloma without BPV antigen...
September 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210093/hydropericardium-syndrome-caused-by-fowl-adenovirus-serotype-4-in-replacement-pullets
#11
Zhen Chen, Shaohua Shi, Baoming Qi, Su Lin, Cuiteng Chen, Chunhua Zhu, Yu Huang
Hydropericardium syndrome (HPS) is one of the important emerging diseases causing huge losses to the poultry industry. It affects mainly 3- to 6-week-old broiler chickens and rarely occurs in breeding and laying flocks. Recently, an HPS case was recorded with a sudden heavy mortality in a 100-day-old laying flock. A fowl adenovirus serotype 4 (FAdV-4), named as GDMZ strain, was isolated and identified using polymerase chain reaction coupled with electron microscopy. The animal experiment showed that a mortality of 100% was recorded with hydropericardium as a conspicuous lesion throughout the course of infection...
September 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210092/seroprevalence-of-bovine-arboviruses-belonging-to-genus-orthobunyavirus-in-south-korea
#12
Kyoungah Jun, Tadashi Yanaka, Kun-Kyu Lee, Joong-Bok Lee
Arboviruses, belonging to the Simbu serogroup of the genus Orthobunyavirus, often cause congenital malformations and reproductive loss in cattle. The recent occurrences of such reproductive problems suggest that new arboviruses have emerged in Japan. However, there is no information on the presence of these viruses in South Korea. The aim of this study was to determine the presence of antibodies for Akabane, Aino, Peaton, Sathuperi, and Shamonda viruses in four regions, namely Gyeonggi, Jeollabuk, Jeollanam provinces, and Jeju Island of South Korea by a serum neutralization test...
September 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210069/system-for-the-heterologous-expression-of-ns1-protein-of-h9n2-avian-influenza-virus-in-the-ciliate-tetrahymena-thermophila
#13
Chunhua Zhu, Xiaodong Liu, Hongshu Chi, Cuiteng Chen, Zhen Chen, Guanghua Fu, Hui Gong, Yu Huang
Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system...
September 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210068/independent-chondrogenic-potential-of-canine-bone-marrow-derived-mesenchymal-stem-cells-in-monolayer-expansion-cultures-decreases-in-a-passage-dependent-pattern
#14
Eugene C Bwalya, Hm Suranji Wijekoon, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
Although chondroinductive growth factors are considered necessary for chondrogenesis of bone marrow-derived mesenchymal stem cells (BMSC), independent and spontaneous chondrogenesis has been previously demonstrated in adult horses, bovine calves and adult human BMSC. Surprisingly, adult canine BMSC under similar culture conditions previously failed to demonstrate chondrogenesis. The present study evaluated independent chondrogenic potential of BMSC sourced from three young dogs in the absence of known chondroinductive factors...
September 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210067/influence-of-swine-leukocyte-antigen-haplotype-on-serum-antibody-titers-against-swine-erysipelas-vaccine-and-reproductive-and-meat-production-traits-of-sla-defined-selectively-bred-duroc-pigs
#15
Noriaki Imaeda, Asako Ando, Masaki Takasu, Tatsuya Matsubara, Naohito Nishii, Satoshi Takashima, Atsuko Shigenari, Takashi Shiina, Hitoshi Kitagawa
We investigated possible associations of SLA class II haplotypes with serum antibody titers against swine erysipelas vaccine, reproductive and meat production traits using a population of selective breeding Duroc pigs. In the selective breeding Duroc pigs, four SLA class II-DRB1 and -DQB1 alleles were assigned by using PCR- sequence specific primer technique. Low-resolution haplotype (Lr)-0.30 and/or Lr-0.13, were deduced from the SLA class II alleles in the population of SLA-defined Duroc pigs. SLA-homozygous piglets with the Lr-0...
September 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30210066/protective-antibody-response-of-balb-c-mice-to-bali-rabies-virus-isolate-propagated-in-bhk-21-cells
#16
I Nyoman Mantik Astawa, Ni Luh Putu Agustini, I Wayan Masa Tenaya, I Putu Gede Widnyana Aryawiguna
The protective antibody response of Balb/c mice to Bali rabies virus (RABV) in BHK-21 cells was studied. The virus was isolated from a rabid dog and was adapted to replicate in BHK-21 cell culture for seven passages. The BHK-21-adapted Bali RABV (BHK-Bali RABV) was inactivated with binary ethylenimine and 24 mice were immunized twice at 21-days intervals with the inactivated virus and Rabisin® vaccine. Virus replication was detected using indirect immunofluorescence, immunocytochemistry, and western blotting assays...
September 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30185722/candida-parapsilosis-and-candida-tropicalis-infections-in-an-okhotsk-snailfish-liparis-ochotensis
#17
Mohie Haridy, Walied Abdo, Mahmoud Hashem, Tokuma Yanai
An Okhotsk snailfish (Liparis ochotensis) kept at Nagoya aquarium exhibited sudden death. Microscopically, the fish showed multiple granulomatous foci in the gills, liver and kidney. Multiple yeast-like organisms as well as pseudohyphal elements were observed within granulomatous lesions. Immunohistochemically, the hyphae were negative for both Asperigullus and Mucor spp., and a weak positive for Candida sp. The seminated-PCR product was consistent with Candida parapsilosis and C. tropicalis. This is the first record of disseminated mycotic granulomatous lesion due to C...
September 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30185721/anaplastic-oligodendroglioma-with-leptomeningeal-dissemination-in-a-french-bulldog
#18
Yuya Nakamoto, Daisuke Fukunaga, Kazuyuki Uchida, Takashi Mori, Takuya Kishimoto, Tsuyoshi Ozawa
A 2.5-year-old male French Bulldog was evaluated for seizures. Cranial magnetic resonance imaging (MRI) suggested a glioma in the left piriform area. Radiation therapy (RT) and continuous chemotherapy were administered. Although the lesion had regressed significantly 2 months after RT, a follow-up MRI revealed meningeal enhancement in the left piriform area, which expanded further, with hydrocephalus, by day 310 (8 months after RT). Because of the poor prognosis, the dog was euthanized on day 356 and necropsy was performed...
September 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30185725/uterine-metastatic-rhabdomyosarcoma-in-a-scimitar-horned-oryx-oryx-dammah
#19
Du-Min Go, Sang-Ho Woo, Su-Hyung Lee, Soo-Whan Kwon, Dong-Hee Chung, Soong-Hee Youn, Eun Jung, Dae-Yong Kim
A 13-year-old female scimitar-horned oryx (Oryx dammah) died after progressive anorexia, weight loss, and depression. The necropsy showed that the retroperitoneum was compressed by a large white-to-tan uterine mass and on several sections of the mass, the uterine wall was markedly thickened because of ill-defined transmural tumor tissue. Metastatic nodules were detected in the omentum, mesentery, diaphragm, and lung. The genital tract and pulmonary and abdominal nodules exhibited highly pleomorphic sarcoma...
September 5, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30185724/detection-of-serum-antibodies-to-brucella-in-russian-aquatic-mammals
#20
Kazue Ohishi, Erika Abe, Masao Amano, Nobuyuki Miyazaki, Andrei Boltunov, Etsuko Katsumata, Tadashi Maruyama
A serologic survey of Brucella infection was performed in Caspian seals (Pusa caspica, n=71), Baikal seals (P. sibirica, n=7), ringed seals (P. hispida hispida, n=6), and beluga whales(Delphinapterus leucas, n=4) inhabiting Russian waters, by enzyme-linked immunosorbent assay (ELISA) using Brucella abortus and B. canis as antigens. The sera of 4 Caspian seals (4%) tested positive for B. abortus. The same sera samples demonstrated weaker yet detectable affinity for B. canis antigens. Several discrete bands against B...
September 5, 2018: Journal of Veterinary Medical Science
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