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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/27890868/serological-validation-of-an-alveolar-echinococcosis-rat-model-with-a-single-hepatic-lesion
#1
Masamichi Yamashita, Tomohiro Imagawa, Yasuhito Sako, Munehiro Okamoto, Tetsuya Yanagida, Yoshiharu Okamoto, Takeshi Tsuka, Tomohiro Osaki, Akira Ito
Serology is important for the diagnosis and follow-up of human alveolar echinococcosis (AE). However, patient conditions are highly variable among those with AE, and antibody responses in serological follow-up have not been well-defined. We recently described a new AE rat model established by implantation of small AE tissue into a single arbitrary location in the liver; no metastasis and dissemination were observed. In the present study, we examined the serological characteristics in our rat model before and after surgical treatment...
November 27, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890889/first-molecular-detection-of-tick-borne-pathogens-in-dogs-from-jiangxi-china
#2
Weiqing Zheng, Mingming Liu, Paul Franck Adjou Moumouni, Xiaoqing Liu, Artemis Efstratiou, Zhanbin Liu, Yangqing Liu, Huiying Tao, Huanping Guo, Guanbo Wang, Yang Gao, Zifen Li, Aaron Edmund Ringo, Charoonluk Jirapattharasate, Haiying Chen, Xuenan Xuan
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp...
November 26, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890888/cloning-and-expression-of-a-novel-catechol-o-methyltransferase-in-common-marmosets
#3
Shotaro Uehara, Yasuhiro Uno, Takashi Inoue, Erika Sasaki, Hiroshi Yamazaki
Catechol-O-methyltransferase (COMT) catalyzes the O-methylation of endogenous catechol amines and estrogens and exogenous catechol-type of drugs. A Parkinson's disease model of common marmoset (Callithrix jacchus) has been widely used in preclinical studies to evaluate inhibitory potential of new drug candidates on marmoset COMT. Despite COMT inhibitors could potentiate the pharmacological action of levodopa on Parkinson's disease in animal models, marmoset COMT cDNA has not yet been identified and characterized...
November 26, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890887/royal-jelly-improves-hyperglycemia-in-obese-diabetic-kk-ay-mice
#4
Mei Yoshida, Kaori Hayashi, Risa Watadani, Yoshiyasu Okano, Keiya Tanimura, Jun Kotoh, Daiki Sasaki, Kozo Matsumoto, Akihiko Maeda
The study examined whether royal jelly (RJ) can prevent obesity and ameliorate hyperglycemia in type 2 diabetes. This study utilized obese/diabetic KK-Ay mice. RJ (10 mg/kg) was administered by oral gavage. Body weight, plasma glucose and insulin levels were measured. mRNA and protein levels were determined using quantitative reverse transcription polymerase chain reaction and western blotting, respectively. Four weeks of RJ administration improved hyperglycemia and partially suppressed body weight gain, although the latter effect did not reach statistical significance...
November 26, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890906/accuracy-of-the-evaluation-method-for-alkaline-agents-bactericidal-efficacies-in-solid-and-the-required-time-of-bacterial-inactivation
#5
Hakimullah Hakim, Chiharu Toyofuku, Mari Ota, Mayuko Suzuki, Miyuki Komura, Masashi Yamada, Md Shahin Alam, Natthanan Sangsriratanakul, Dany Shoham, Kazuaki Takehara
An alkaline agent, namely food additive grade calcium hydroxide (FdCa(OH)2) in the powder form, was evaluated for its bactericidal efficacies in chicken feces at pH 13. The point for this evaluation was neutralization of the alkaline agent's pH at the time of bacterial recovery, since otherwise the results are substantially misleading. Without neutralization of the FdCa(OH)2 pH, the spiked bacteria were killed within min at the time of recovery in aqueous phase, but not in the solid form in feces, hence it has been demonstrated that when bacteria were in solid, it took longer time than in liquid for the alkaline agent to inactivate them down to the acceptable level (≥3 log10 CFU/ml)...
November 25, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890905/bone-morphogenetic-protein-1-is-expressed-in-porcine-ovarian-follicles-and-promotes-oocyte-maturation-and-early-embryonic-development
#6
Xiaocan Lei, Kuiqing Cui, Xiaoyan Cai, Yanping Ren, Qingyou Liu, Deshun Shi
In the present study, we tried to determine whether bone morphogenetic protein 1 (BMP1) plays a role in ovarian follicular development and early embryo development. We systematically investigated the expression and influence of BMP1 during porcine follicle and early embryonic development. Immunohistochemistry demonstrated that the BMP1 protein is expressed in granular cells and oocytes during follicular development, from primary to pre-ovulatory follicles, including atretic follicles and the corpus luteum. The mRNA expression of BMP1 significantly increased as the porcine follicles grew...
November 25, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27890904/pharmacokinetic-studies-of-the-recombinant-chicken-interferon-%C3%AE-in-broiler-chickens
#7
Jun Zhao, Hai-Yang Yu, Jun-Ling Zhang, Xing-Man Wang, Jin-Pei Li, Tao Hu, Yong Hu, Ming-Li Wang, Yong-Zhou Shen, Jing-Dong Xu, Guo-Xiang Han, Jason Chen
In this study, 24 male and female broiler chickens at 30-day-old were divided into three groups with 8 animals in each group. The animals were administered with recombinant chicken interferon-α (rChIFN-α) at a dose of 1.0× 10(6) IU/kg intravenously, intramuscularly or subcutaneously, respectively. Serum samples were collected at different time points post administration, and the titers of rChIFN-α in the blood were determined by cytopathic effect inhibition assay. The results showed that the pharmacokinetic characteristics of rChIFN-α by intramuscular injection and subcutaneous injection were fitted to one compartment open model, and the Tmax was 3...
November 25, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27885217/lymphocytic-adrenal-medullitis-and-lymphocytic-thyroiditis-in-a-laboratory-beagle-dog
#8
Takuya Doi, Yuki Tomonari, Kazufumi Kawasako, Naoaki Yamada, Minoru Tsuchitani
Lymphocytic adrenal medullitis characterized by inflammation and atrophy in the medulla of the bilateral adrenal glands was observed in an 18-month-old male laboratory beagle dog. It might be that the present lymphocytic adrenal medullitis is an autoimmune-mediated disease as the histological characteristics are consistent with an autoimmune pathogenesis. However, the actual cause remains unclear as the existence of serum autoantibodies against the adrenal medulla could not be confirmed. Although this dog also contracted lymphocytic thyroiditis along with serum thyroglobulin autoantibodies, indicating that the thyroiditis occurred with an autoimmune basis; the relation between the adrenal medullitis and thyroiditis is unknown...
November 24, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27867163/anatomical-variations-of-the-arterial-supply-to-the-adrenal-gland-in-the-rat
#9
Tetsuhito Kigata, Hideshi Shibata
The adrenal gland is an essential endocrine organ for the stress response. The functions of this organ may be studied by ligation of the adrenal artery or adrenalectomy. However, in prior studies, descriptions of the anatomical variations of the adrenal artery were insufficient and inconsistent. Therefore, anatomical variations of the arterial supply to the adrenal gland were studied in 18 male and 18 female Wistar rats by colored latex injection into the arteries. The vascularization pattern was categorized into 4 types based on the origin of each adrenal artery...
November 19, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27853047/long-term-management-of-a-dog-with-idiopathic-megaesophagus-and-recurrent-aspiration-pneumonia-by-use-of-an-indwelling-esophagostomy-tube-for-suction-of-esophageal-content-and-esophagogastric-tube-feeding
#10
Yuka Kanemoto, Kenjiro Fukushima, Hideyuki Kanemoto, Koichi Ohno, Hajime Tsujimoto
A 10-year-old neutered male Labrador Retriever dog was diagnosed with idiopathic megaesophagus. Despite receiving conventional treatments including elevated feeding, the dog showed repeated regurgitation and aspiration pneumonia, consequently developing weight loss and severe malnutrition. For the purpose of controlling regurgitation, an esophagostomy tube was placed for draining the esophageal fluid. Additionally, an esophagogastric tube was placed for nutritional support. After tube placement, the average frequency of regurgitation was reduced from 2...
November 17, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27840394/characterization-of-canine-coronavirus-spread-among-domestic-dogs-in-vietnam
#11
Dung VAN Nguyen, Yukata Terada, Shohei Minami, Kenzo Yonemitsu, Nao Nagata, Thanh Dinh Ha LE, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda
Canine coronavirus (CCoV) is an important pathogen that causes enteritis in dogs, but there is no information on CCoV infection in Vietnam. To examine the prevalence of CCoV infection among Vietnamese dogs, 201 serum samples were analyzed by virus-neutralization (VN) test. The results showed that antibody against CCoV-II was present in 87 dogs (43.3%). To detect genes of CCoV, fecal samples collected from 30 diarrheic and 50 healthy dogs were examinated by RT-PCR, confirming that 2 diarrheic dogs and 5 healthy dogs were positive for CCoV...
November 14, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27840393/the-full-genome-sequences-of-8-equine-herpesvirus-type-4-isolates-from-horses-in-japan
#12
Satoko Izume, Rikio Kirisawa, Kenji Ohya, Aiko Ohnuma, Takashi Kimura, Tsutomu Omatsu, Yukie Katayama, Tetsuya Mizutani, Hideto Fukushi
Equine herpesvirus type 4 (EHV-4) is one of the most important pathogens in horses. To clarify the key genes of the EHV-4 genome that cause abortion in female horses, we determined the whole genome sequences of a laboratory strain and 7 Japanese EHV-4 isolates that were isolated from 2 aborted fetuses and nasal swabs of 5 horses with respiratory disease. The full genome sequences and predicted amino acid sequences of each gene of these isolates were compared with of the reference EHV-4 strain NS80567 and Australian isolates that were reported in 2015...
November 14, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27840377/evaluation-of-glutathione-binding-protein-a-of-haemophilus-parasuis-as-a-vaccine-candidate-in-a-mouse-model
#13
Yanhe Zhang, Gang Li, Fang Xie, Siguo Liu, Chunlai Wang
The virulent strains of Haemophilus parasuis are the causative agents of Glässer's disease, which can cause systemic infection and result in polyserositis, meningitis, and arthritis. The development of novel, effective vaccines would be beneficial to preventing H. parasuis infections. Here, we report a novel immunogenic protein, glutathione-binding protein A (GbpA), which can elicit a significant humoral antibody response and confer significant protection against challenge with a lethal dose of a highly virulent H...
November 11, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27840376/mobility-of-the-forearm-in-the-raccoon-procyon-lotor-raccoon-dog-nyctereutes-procyonoides-and-red-panda-ailurus-fulgens
#14
Minao Kamioka, Motoki Sasaki, Kazutaka Yamada, Hideki Endo, Motoharu Oishi, Kazutoshi Yuhara, Sohei Tomikawa, Miki Sugimoto, Tatsuo Oshida, Daisuke Kondoh, Nobuo Kitamura
The ranges of pronation/supination of forearms in raccoons, raccoon dogs and red pandas were nondestructively examined. Three carcasses of each species were used for CT analysis, and the left forearms were scanned with a CT scanner in two positions: maximal supination and maximal pronation. Scanning data were reconstructed into three-dimensional images, cross-sectional images were extracted at the position that shows the largest area in the distal part of ulna, and then, the centroids of each cross section of the radius and ulna were detected...
November 11, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27818461/reliability-of-clinical-diagnosis-and-laboratory-testing-techniques-currently-used-for-identification-of-canine-parvovirus-enteritis-in-clinical-settings
#15
Mirna Faz, José Simón Martínez, Israel Quijano-Hernández, Raúl Fajardo
Canine parvovirus type 2 (CPV-2) is the main etiological agent of viral enteritis in dogs. Actually in literature, CPV-2 has been reported with clinical signs that vary from the classical disease, and immunochromatography test and PCR technique have been introduced to veterinary hospitals to confirm CPV-2 diagnosis and other infections. However, the reliability of these techniques has been poorly analyzed. In this study, we evaluated the sensitivity and specificity of veterinary clinical diagnosis, immunochromatography test and PCR technique...
November 6, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27818458/cartilaginous-choristoma-of-the-lip-in-a-dog
#16
Su-Hyung Lee, Du-Min Go, Sang-Ho Woo, Jee-Yong Eun, Dae-Yong Kim
A six-year-old castrated male Maltese dog presented to a private animal clinic with a mass on the dog's lower lip without any other clinical signs. The mass (3 × 2 × 2 cm) was whitish and grossly well circumscribed, and a histopathological examination revealed that the mass was composed of normal cartilage tissue surrounded by fibrous connective tissues. Based on the gross findings, histopathology and anatomical location of the mass, the first diagnosis of a cartilaginous choristoma in a dog was made.
November 5, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27818457/isolation-and-characterization-of-equine-dental-pulp-stem-cells-derived-from-thoroughbred-wolf-teeth
#17
Shingo Ishikawa, Chie Horinouchi, Daiki Murata, Shota Matsuzaki, Kazuhiro Misumi, Yohei Iwamoto, Kenji Korosue, Seiji Hobo
Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth...
November 5, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27795461/epizootiological-study-of-rodent-borne-hepatitis-e-virus-hev-c1-in-small-mammals-in-hanoi-vietnam
#18
Satomu Obana, Kenta Shimizu, Kumiko Yoshimatsu, Futoshi Hasebe, Kozue Hotta, Rie Isozumi, Hoa Thuy Nguyen, Mai Quynh LE, Tetsu Yamashiro, Yoshimi Tsuda, Jiro Arikawa
There is concern about the zoonotic potential of rodent-borne hepatitis E virus, designated as HEV-C1. However, epizootiological information about HEV-C1 is limited. To address this issue, serum samples from 443 small mammals captured at 5 sites in Hanoi, Vietnam, were examined for anti-HEV-C1 IgG antibodies. In addition, livers of seropositive animals were examined for viral RNA. Anti-HEV-C1 antibodies were detected in 57 (12.9%) of the 443 serum samples. Seropositive animals were found in all of the sites (4...
October 31, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27795464/preparation-of-a-cell-line-persistently-infected-with-maedi-visna-virus-and-production-of-viral-antigens
#19
Kazuya Suzuki, Keisuke Oguma, Hiroshi Sentsui
We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat, and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R 127 cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum...
October 30, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27795463/histopathological-changes-in-the-pancreas-of-cattle-with-abdominal-fat-necrosis
#20
Chikako Tani, Watanyoo Pratakpiriya, Mineto Tani, Takenori Yamauchi, Takuya Hirai, Ryoji Yamaguchi, Hitoshi Ano, Hiromu Katamoto
The association between pancreatic disorder and abdominal fat necrosis in cattle remains unclear. The pancreases of 29 slaughtered cattle with or without fat necrosis were collected to investigate pathological changes. Japanese Black (JB) cattle were classified into the FN group (with abdominal fat necrosis; n=9) and N group (without fat necrosis; n=5). The pancreases were also collected from 15 Holstein Friesian (HF) cows. All JB cattle showed high body condition scores. Regarding the pathological findings, fatty pancreas which involves adipocyte infiltration into the pancreas and fat necrosis (saponification) were observed in 25 and 27 cases, respectively...
October 30, 2016: Journal of Veterinary Medical Science
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