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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/30643105/relationship-between-motor-function-and-ultrasonic-vocalizations-induced-by-maternal-separation-in-rat-pups
#1
Anna Isobe, Maiko Kawaguchi
When rat pups are isolated from their mothers, they emit ultrasonic vocalizations (USVs). Although previous studies have reported that USVs are related to anxiety, others have reported that they are related to simple, nonemotional factors, such as physiological reactions to coldness. In this study, we examined the influence of three maternal separations on rat pups. The number of USVs during 5 min of USV test under maternal separation, latency in the righting reflex as motor function, and body temperature were recorded twice (the first and second tests) before and after the pups were put in various environments for 10 min...
January 15, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30643104/effects-of-genetic-background-on-responses-to-superovulation-in-japanese-black-cattle
#2
Hiroki Hirayama, Akira Naito, Takashi Fujii, Masahito Sugimoto, Toshiro Takedomi, Satoru Moriyasu, Hitomi Sakai, Soichi Kageyama
We investigated the effects of genetic background on the responses to superovulation in Japanese Black cattle. The genotype frequencies of GRIA1 and FSHR relating to ovulation and follicular development in each of the major bloodlines-Tajiri, Fujiyoshi, and Kedaka-were analyzed. The Tajiri line had the lowest frequency of G allele homozygosity of c.710A>G in GRIA1 among the three bloodlines, and deviation from Hardy-Weinberg equilibrium was detected. Genotype frequencies of c.337C>G, c.871A>G, and c...
January 15, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30643103/intra-articular-botulinum-toxin-a-bont-a-for-pain-management-in-dogs-with-osteoarthritis-secondary-to-hip-dysplasia-a-randomized-controlled-clinical-trial
#3
Gabriel Montoro Nicácio, Stelio Pacca Loureiro Luna, Poliana Cavaleti, Renata Navarro Cassu
The aim of this study was to evaluate the efficacy and safety of the intra-articular (IA) injection of botulinum toxin type A (BoNT/A) to the management of chronic pain in dogs. In a randomized, controlled, double-blinded study sixteen dogs with osteoarthritis secondary to hip dysplasia were distributed into two groups: 25 IU BoNT/A (BoNT) or saline solution (Control) was administered IA in each affected joint. All dogs received oral supplements (90 days) and carprofen (15 days). The dogs were assessed by a veterinarian on five occasions and the owner completed an assessment form at the same time (baseline to 90 days)...
January 15, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30643102/persistent-hyperplastic-primary-vitreous-in-two-piglets
#4
Tomoaki Murakami, Tomoyuki Miyoshi, Natsumi Takahashi, Akihisa Kangawa
Two Large Yorkshire piglets were diagnosed as persistent hyperplastic primary vitreous (PHPV). In case 1, the white cord-like structure extending from optic disc to lens was observed in the normal-sized right eye. Case 2 showed buphthalmos of the right eye. The internal structure of the right eye was unclear due to bleeding, but a white cord-like structure was slightly observed. In both cases, histological examinations revealed the fibrovascular cord-like structure in hyaloid vitreous. The retina was detached, and dysplastic nervous tissue was observed in anterior vitreous...
January 15, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30626762/clinical-features-and-prognosis-of-canine-megaesophagus-in-japan
#5
Taisuke Nakagawa, Akihiro Doi, Koichi Ohno, Nozomu Yokoyama, Hajime Tsujimoto
Megaesophagus (ME) is a common esophageal disease in dogs and the prognosis is generally poor, especially with aspiration pneumonia (AP). We retrospectively investigated the clinical features and prognosis of canine ME in Japan. Twenty-eight dogs were included in this study, with the Miniature Dachshund breed being significantly overrepresented (odds ratio: 4.33). Most cases (21 of 28) were diagnosed as idiopathic ME and Myasthenia gravis (MG) was the most common cause of secondary ME. The overall median survival time (MST) was not reached and the 3-month survival rate was 85...
January 10, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606893/temporal-changes-of-abomasal-contents-and-volumes-in-calves-fed-milk-diluted-with-oral-rehydration-salt-solution
#6
Tamako Miyazaki, Keiji Okada, Tetsuro Yamashita, Masao Miyazaki
Several manufacturers recommend to feed mixture comprising equal amounts of oral rehydration salt (ORS) solution and milk for diarrheic calves after milk withdrawal. Such a feeding method is expected to supply more nutrients and energy compared to feeding only the ORS solution. However, little is known about the effects of feeding milk diluted with ORS solution on calves' digestive process. This study examined the abomasal contents, volumes, and emptying rates in calves fed whole milk, milk diluted by 50% with ORS solution (50% ORS-milk), and ORS solution...
January 3, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606907/septicemic-actinobacillus-suis-infection-in-a-neonatal-piglet-with-multifocal-necrotic-glossitis
#7
Kennosuke Sugie, Tetsuya Komatsu, Eri Watando, Nanami Inaba, Kiyoko Kato, Daisuke Takamatsu, Hiroya Ito, Tomoyuki Shibahara
Five-day-old neonatal piglets presented with debilitation and ananastasia. At the necropsy of one piglet, the apex of the tongue was found to be discolored dark red, and disseminated white foci were found on the cut surface. Many white foci were also found in the lungs and on the serosa of the liver and spleen. Histopathological findings revealed multifocal necrotic glossitis and pneumonia with Gram-negative bacilli. The bacilli were identified as Actinobacillus suis through immunohistochemical, biochemical, and genetic tests, including 16S rRNA gene sequencing...
January 1, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606906/poxvirus-infection-in-a-steller-s-sea-eagle-haliaeetus-pelagicus
#8
Keisuke Saito, Mohie Haridy, Walied Abdo, Ahmed El-Morsey, Samy Kasem, Yukiko Watanabe, Tokuma Yanai
A severely emaciated adult Steller's sea eagle (Haliaeetus pelagicus ) was found dead with electrocution-induced severe wing laceration, with multiple cutaneous pock nodules at the periocular regions of both sides nearby the medial canthi and rhamphotheca. Histopathological examination of the nodules revealed hyperplasia of the epidermis with vacuolar degeneration and intracytoplasmic inclusion bodies (Bollinger bodies). The proventriculus was severely affected by nematodes and was ulcerated. Nucleotide sequencing of a PCR-amplified product of Avipoxvirus 4b core gene revealed 100% identity to the sequence of Avipoxvirus derived from other eagle species...
January 1, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606905/methodological-improvement-of-fluorescein-isothiocyanate-peanut-agglutinin-fitc-pna-acrosomal-integrity-staining-for-frozen-thawed-japanese-black-bull-spermatozoa
#9
Reza Rajabi-Toustani, Quzi Sharmin Akter, Essam A Almadaly, Yoichiro Hoshino, Hiromichi Adachi, Koushi Mukoujima, Tetsuma Murase
This study aimed to improve the staining of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA). Spermatozoa were washed, fixed with 1-3% paraformaldehyde (PFA) in suspension for 10, 20, and 30 min, permeabilized with 0-2% Triton X-100 for 5 min, stained with FITC-PNA, and mounted with different antifade agents (0.22 M 1,4-diazabicyclo [2,2,2] octane (DABCO), SlowFade® , and ProLong® ) in suspension (In-suspension) or on a smear (On-smear)...
January 1, 2019: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606914/feline-type-cystic-basal-cell-tumor-filled-with-abundant-melanin-pigment-rich-fluid-in-a-dog
#10
Seoung-Woo Lee, Hoon Ji, Su-Min Baek, A-Rang Lee, Min-Ji Kim, Sang-Joon Park, Seong-Kyoon Choi, Sungho Yun, Tae-Hwan Kim, Kyu-Shik Jeong, Jin-Kyu Park
A 2-year-old castrated male mongrel dog presented with a well-demarcated fluctuant dermal mass, located on the back of the neck. Grossly along with cystic structures filled with a black greasy fluid, when cut open. Microscopically, the mass was multi-lobulated. The lobules consisted of neoplastic basaloid cells and showed central degeneration, forming multiple central cystic structures filled with dark melanin-pigmented materials. Immunohistochemically, the neoplastic cells were strongly positive for CK14 and partially positive for CK19, but negative for CK7, CK8/18, CD34, S-100, Melan-A and α-SMA...
December 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606892/successful-embryo-transfer-from-hokkaido-native-pony-after-artificial-insemination-with-frozen-semen
#11
M A Hannan, Shingo Haneda, Yasuyuki Itami, Sohei Wachi, Tomoko Saitoh, Soon Hon Cheong, Yasuo Nambo
There has been no report of equine embryo transfer in Japan for the last 24 years. Our objective was to establish an effective protocol for embryo transfer in domestic horse. A Hokkaido native pony was bred by deep-horn artificial insemination with frozen semen from a Connemara pony. Embryo collection was performed using a non-surgical method on day 7. Two embryos were obtained from three flushes (67% recovery) and were transferred fresh into crossbred recipient mares. Both recipient mares were diagnosed pregnant (100% pregnancy rate) 5 days after embryo transfer and had normal progesterone levels until the end of the observation on day 35 of gestation...
December 28, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606891/measurement-of-plasma-endothelin-1-concentration-in-healthy-horses-and-horses-with-cardiac-disease-during-rest-and-after-exercise
#12
Heidrun Gehlen, Tarek Shety, Heba El-Zahar, Ina Hofheinz
Cardiac biomarkers are important tools for monitoring disease progress and can monitor progression of therapy. Endothelin-1 (ET-1) has been studied for its use as a cardiac biomarker in human and small animal medicine while in horses with cardiac disease it has not been evaluated yet. The objective of the present study was to determine the concentration of plasma ET-1 in healthy horses and compare it with ET-1 concentration in horses with cardiac disease during rest and after exercise. 54 horses admitted to the Equine Clinic of Free University of Berlin were used in the present study, of which 15 horses were clinically healthy with no evidence of cardiac disease (Group 1), 22 horses suffered from cardiac disease with normal heart dimensions (Group 2) and 17 horses with cardiac disease and enlarged heart diameters (Group 3)...
December 28, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30606890/a-specific-laryngeal-finding-in-dogs-with-bronchial-vegetal-foreign-bodies-a-retrospective-study-of-63-cases
#13
Maria Chiara Marchesi, Domenico Caivano, Maria Beatrice Conti, Francesca Beccati, Laura Valli, Sara Busechian, Fabrizio Rueca
Grass awns commonly cause respiratory disease in dogs; when located in the trachea or bronchi, they cause severe bronchial inflammation and sepsis. The interplay of cough, bronchoconstriction, and mucus secretion can result in a less effective expulsive cough phase, especially when the causal factor persists. The bronchial exudate could consequently become trapped in the upper respiratory tracts of dogs with bronchial vegetal foreign bodies. We retrospectively reviewed endoscopic findings of the upper respiratory tract in dogs that underwent bronchoscopy in our hospital and correlated these findings with the presence of bronchial grass awns...
December 28, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30587674/pre-pubertal-exposure-to-high-temperature-impairs-ovarian-and-adrenal-gland-function-in-female-rats
#14
Meihua Zheng, Kentaro Nagaoka, Gen Watanabe
The influence of different levels of heat exposure on the functions of ovarian and adrenal gland were investigated in pre-puberty female rats. Three-week old female rats were treated with control (26℃) or three higher temperatures (38, 40 and 42℃) for 2hr/day. After 9 days of treatment, blood samples were collected for measurement of luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol-17β, corticosterone, cholesterol and triglyceride. Adrenal glands, ovaries and liver were collected for analyzing gene expressions...
December 27, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30587673/serological-and-histological-evaluation-of-species-specific-immunocontraceptive-vaccine-antigens-based-on-zona-pellucida-3-in-the-small-indian-mongoose-herpestes-auropunctatus
#15
Naotoshi Kuninaga, Makoto Asano, Ryota Matsuyama, Takahiro Minemoto, Takayuki Mori, Masatsugu Suzuki
The small Indian mongoose (Herpestes auropunctatus) was introduced to Japanese islands and has impacted on the island's biodiversity. Population control has been attempted through capturing but its efficiency has rapidly declined. Therefore, new additional control methods are required. Our focus has been on the immunocontraceptive vaccines, which act in an especially species-specific manner. The amino-acid sequence of the mongoose ovum zona pellucida protein 3 (ZP3) was decoded and two types of synthetic peptides (A and B) were produced...
December 27, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30587672/a-review-poisoning-by-anticoagulant-rodenticides-in-non-target-animals-globally
#16
Shouta M M Nakayama, Ayuko Morita, Yoshinori Ikenaka, Hazuki Mizukawa, Mayumi Ishizuka
Worldwide use of anticoagulant rodenticides (ARs) for rodents control has frequently led to secondary poisoning of non-target animals, especially raptors. In spite of the occurrence of many incidents of primary or secondary AR-exposure and poisoning of non-target animals, these incidents have been reported only for individual countries, and there has been no comprehensive worldwide study or review. Furthermore, the AR exposure pathway in raptors has not yet been clearly identified. The aim of this review is therefore to comprehensively analyze the global incidence of primary and secondary AR-exposure in non-target animals, and to explore the exposure pathways...
December 27, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30587669/reconstruction-of-urethral-defects-using-a-fascia-lata-autograft-in-a-dog
#17
Da-Eun Lee, Kyu-Chang Kim, Soon-Wuk Jeong, Hun-Young Yoon
A castrated male Shih-Tzu dog was presented for evaluation of urine leakage after urethrotomy. A fistula with urine leakage was identified in the perineal region. On retrograde urethrography, the contrast extravasated from the penile urethra into the subcutaneous tissue of the perineum. Urine leakage with urethrocutaneous fistula was diagnosed. During surgery, the disrupted urethra wall and two urethral defects were identified. A fascia lata autograft was used, rather than primary repair of the urethra. Two pieces of fascia lata were harvested and sutured to the urethral defects...
December 26, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30584200/a-recombinant-adenovirus-expressing-the-e-protein-of-duck-tembusu-virus-induces-protective-immunity-in-duck
#18
Jingyu Tang, Dongdong Yin, Rui Wang, Qi Zhou, Xiaoya Zhou, Xue Xing, Hong-Mei Liu, Guangqing Liu, Guijun Wang
Duck Tembusu virus disease, caused by the duck Tembusu virus (DTMUV), can lead to a severe reduction in egg production and growth retardation in laying ducks and ducklings, respectively. In this study, we engineered a novel recombinant adenovirus expressing the E protein of DTMUV (rAd-E) in AAV-293 cells (analyzed by western blot and indirect immunofluorescence assays). Intramuscular immunization of Cherry Valley ducks with rAd-E was performed to evaluate host cellular and humoral immune responses. Compared to the phosphate-buffered saline administered group and the negative control wild-type adenovirus (wtAd) group, the rAd-E vaccinated group showed increased cellular and humoral responses...
December 24, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30584199/evaluation-of-atlantooccipital-overlapping-and-cerebral-ventricle-size-in-dogs-with-atlantoaxial-instability
#19
Fumitaka Takahashi, Shigenori Kouno, Shinya Yamaguchi, Yasushi Hara
This study investigated cerebral ventricle size and concurrent craniocervical junction abnormality in relation to atlantooccipital overlapping (AOO) in dogs with atlantoaxial instability (AAI). A total of 61 dogs were treated with atlantoaxial ventral fixation. Medical records of each dog, including magnetic resonance (MR) and computed tomography (CT) images, were retrospectively reviewed. CT images were assessed for the presence of AOO and the dogs were then assigned to either an AOO group or a non-AOO group accordingly...
December 24, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/30568107/abdominal-hernias-in-camel-camelus-dromedaries-clinical-findings-and-treatment-outcomes
#20
Madeh Sadan, El-Sayed El-Shafaey, Sabry El-Khodery
The present study was designed to describe the clinical presentation of abdominal hernias and to evaluate the efficacy of polypropylene mesh in repair of such affection in camels. Twenty-six dromedary camels were included in this study on the basis of clinical and ultrasonographic evidence of abdominal hernia. Factors associated with prevalence and clinical findings of hernia were presented and hernioplasty using polypropylene mesh was evaluated as a surgical intervention. Out of 26 studied camels, abdominal hernia was prevalent in Wadeh camels than other breeds (17 vs 9, P<0...
December 20, 2018: Journal of Veterinary Medical Science
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