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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/29780077/the-effect-of-remifentanil-on-the-minimum-alveolar-concentration-mac-and-mac-derivatives-of-sevoflurane-in-dogs
#1
Yusuke Murahata, Yoshiaki Hikasa, Sho Hayashi, Koki Shigematsu, Natsuki Akashi, Tomohiro Osaki, Takeshi Tsuka, Yoshiharu Okamoto, Tomohiro Imagawa
Remifentanil is an ultra-short-acting μ-opioid receptor agonist. The purpose of this study was to determine the relationship of the minimum alveolar concentration (MAC) of sevoflurane and other MAC derivatives, including the MAC for blocking adrenergic responses (MAC-BAR) and the MAC at which tracheal extubation is occurred (MAC-extubation), with or without remifentanil infusion. Six healthy adult beagle dogs were randomly anesthetized three times for determining the MAC-BAR (SEVMAC-BAR ), MAC (SEVMAC ), and MAC- extubation (SEVMAC-extubation ) of sevoflurane under infusion of saline and remifentanil at rates of 0...
May 21, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29780076/the-influence-of-the-duration-of-the-preoperative-time-spent-in-the-veterinary-clinic-without-the-owner-on-the-psychogenic-and-oxidative-stress-in-dogs
#2
Dalia Juodžentė, Birutė Karvelienė, Vita Riškevičienė
The objective of this study was to evaluate the influence of the dog's long-term separation from its owner in the novel environment on the occurrence of psychogenic and oxidative stress. Group I dogs (n=9) were brought to the veterinary clinic and stayed in a kennel room for 12 hr before the surgery, and group II dogs (n=9) - for 10 min before the surgery. Physiological parameters (heart rate (HR) (beats/min) and respiratory rate (fR ) (breaths/min)) were measured and blood sampling was done 12 hr before the surgery (T0) for group I dogs and 10 min before the surgery (T1) for both groups dogs...
May 21, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29780039/a-long-term-animal-experiment-indicating-persistent-infection-of-bovine-coronavirus-in-cattle
#3
Toru Kanno, Ryoko Ishihara, Shinichi Hatama, Ikuo Uchida
A long-term animal experiment involving inoculation with bovine coronavirus (BCoV) was conducted to verify its persistent infection in cattle. Three colostrum-deprived Holstein calves were housed separately in individual rooms of a high-containment facility and inoculated with the BCoV strain Kumamoto/1/07. Until the end of the experiment (1,085, 700 and 280 days, respectively), viral RNAs were detected sporadically by RT-PCR and nested PCR from plasma, nasal discharge, and feces. Seroconversion and titer changes were validated by hemagglutination inhibition tests and neutralization tests...
May 18, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29780038/is-bovine-protothecal-mastitis-related-to-persistent-infection-in-intestine
#4
Tomomi Kurumisawa, Rui Kano, Yuta Nakamura, Maki Hibana, Takaaki Ito, Hiroshi Kamata, Kazuyuki Suzuki
Prototheca zopfii is associated with bovine mastitis, which causes a reduction in milk production and secretion of thin, watery milk with white flakes. However, the source of infection and an infection route of mastitis have not been clarified. In this study, we evaluated the prevalence of P. zopfii genotype 2 in fecal samples from Japanese dairies with or without a history of protothecal mastitis in 2017. P. zopfii genotype 2 was detected in 23 of 60 (38%) fecal samples in only the herd with a history of protothecal mastitis...
May 18, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29769478/mutation-of-the-s-and-3c-genes-in-genomes-of-feline-coronaviruses
#5
Keisuke Oguma, Megumi Ohno, Mayuko Yoshida, Hiroshi Sentsui
Feline coronavirus (FCoV) is classified into two biotypes based on its pathogenicity in cats: a feline enteric coronavirus of low pathogenicity and a highly virulent feline infectious peritonitis virus. It has been suspected that FCoV alters its biotype via mutations in the viral genome. The S and 3c genes of FCoV have been considered the candidates for viral pathogenicity conversion. In the present study, FCoVs were analyzed for the frequency and location of mutations in the S and 3c genes from faecal samples of cats in an animal shelter and the faeces, effusions, and tissues of cats that were referred to veterinary hospitals...
May 17, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29769477/enzyme-immunoassays-for-water-soluble-steroid-metabolites-in-the-urine-and-feces-of-japanese-macaques-macaca-fuscata-using-a-simple-elution-method
#6
Keiko Shimizu, Keiko Mouri
A simple, non-alcoholic extraction method for measuring estrogen and progesterone metabolites in excreta using enzyme immunoassays (EIAs) was developed in Japanese macaques. The obtained detection limits of EIAs using estrone conjugates (E1 C), pregnanediol glucuronide (PdG), and estriol glucuronide (E3 G) polyclonal antibodies with cross-reactivity to urinary and fecal steroid metabolites were 6.6 pg/ml, 2.1 ng/ml, and 0.35 ng/ml, respectively. These assays allowed the determination of E1 C, PdG, and E3 G from the excreta with good reproducibility and accuracy...
May 17, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29769451/effect-of-ferulic-acid-supplementation-on-the-developmental-competence-of-porcine-embryos-during-in-vitro-maturation
#7
Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano, Toshiki Kunihara, Takeshige Otoi
The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes...
May 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29760322/effect-of-dietary-difructose-anhydride-iii-supplementation-on-bone-mineral-density-and-calcium-metabolism-in-late-lactation-dairy-cows
#8
Ayami Maetani, Masayuki Ohtani, Kaoru Hatate, Kotaro Matsumoto, Norio Yamagishi
The aim of the present study was to examine the effect of 28 days of dietary difructose anhydride (DFA) III supplementation on calcium (Ca) metabolism in late-lactation dairy cows. Twenty-four multiparous pregnant Holstein cows were divided into two groups. The DFA group was fed total mixed ration (TMR) supplemented with 40 g of DFA III, and the control group was fed TMR only. The replenishment of bone Ca reserves was evaluated by measuring bone mineral density (BMD) and blood biochemical bone markers. Serum Ca concentrations, urinary Ca-to-creatinine (Cre) (Ca/Cre) ratios, and milk Ca concentrations were also analyzed...
May 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29760315/structural-differences-in-the-brain-between-wild-and-laboratory-rats-rattus-norvegicus-potential-contribution-to-wariness
#9
Ryoko Koizumi, Yasushi Kiyokawa, Kaori Mikami, Akiko Ishii, Kazuyuki D Tanaka, Tsutomu Tanikawa, Yukari Takeuchi
Wild animals typically exhibit defensive behaviors in response to a wider range and/or a weaker intensity of stimuli compared with domestic animals. However, little is known about the neural mechanisms underlying "wariness" in wild animals. Wild rats are one of the most accessible wild animals for experimental research. Laboratory rats are a domesticated form of wild rat, belonging to the same species, and are therefore considered suitable control animals for wild rats. Based on these factors, we analyzed structural differences in the brain between wild and laboratory rats to elucidate the neural mechanisms underlying wariness...
May 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29760314/the-neuroprotective-and-antioxidative-effects-of-submicron-and-blended-lycium-barbarum-in-experimental-retinal-degeneration-in-rats
#10
Jen-Shuai Chang, Yih-Jing Lee, David A Wilkie, Chung-Tien Lin
The object is to determine the neuroprotective and antioxidative effects of submicron and blended Lycium barbarum (LB) on retinal degeneration as evaluated by ERG, retinal histopathology and assays of antioxidant (total GSH) and peroxidant (MDA) in the retina. A rat model of light-induced retinal degeneration was used to assess the protective effect of different forms of Lycium barbarum (LB) on retinal degeneration. Rats were divided into four experimental groups, normal control, light-induced untreated, submicron LB and blended LB treated...
May 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29760313/detection-of-sirtuin-1-protein-expression-in-peripheral-blood-leukocytes-in-dogs
#11
Kuniko Yoshimura, Aya Matsuu, Kai Sasaki, Yasuyuki Momoi
Sirtuin-1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+ )-dependent histone deacetylase with a large number of protein substrates. It has attracted a lot of attention in association with extending lifespan. The objective of this study was to enable the evaluation of SIRT1 expression in peripheral blood mononuclear cells (PBMCs) from dogs by flow cytometry. Three transcript variants were amplified from PBMCs by reverse transcription PCR and the nucleotide sequences were analyzed. On the basis deduced amino acid sequence, a monoclonal antibody against human SIRT1, 1F3, was selected to detect canine SIRT1...
May 11, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29743458/combination-effect-of-allyl-isothiocyanate-and-hoof-trimming-on-bovine-digital-dermatitis
#12
Takumi Yamamoto, Hiroyuki Manabe, Naoaki Misawa, Wataru Yamazaki, Masahiro Takahashi, Keiji Okada
Digital Dermatitis is a localized infectious dermatitis caused by Treponema-like spirochetes. Antibiotics, such as lincomycin, are currently used for treatment, but their use imposes a withdrawal period. This study investigated the therapeutic effect of topical application of the natural component allyl isothiocyanate, in combination with maintenance hoof trimming, on bovine Digital Dermatitis. Study cows were divided into two groups, the Trimming Group and Non-Trimming Group. The day when allyl isothiocyanate was applied, along with hoof trimming, was set as Day 0...
May 9, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29731475/histopathological-study-of-encephalomalacia-in-neonatal-calves-and-application-of-neuronal-and-axonal-degeneration-marker
#13
Kenji Koyama, Akihisa Kangawa, Natsuko Fukumoto, Ken-Ichi Watanabe, Noriyuki Horiuchi, Tomomi Ozawa, Hisashi Inokuma, Yoshiyasu Kobayashi
Five calves that had shown neurological symptoms within 9 days after birth were histopathologically diagnosed as encephalomalacia. Two calves showed bilateral laminar cerebrocortical necrosis and neuronal necrosis in the corpus striatum and hippocampus. Since the distributional pattern of the lesions was consistent with that of global ischemia in other species, the lesions were probably hypoxic/ischemic encephalopathy consistent with the history of dystocia and perinatal asphyxia. One calf also showed bilateral laminar cerebrocortical necrosis...
May 4, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29731474/a-case-of-unusual-persistent-pupillary-membrane-with-total-anterior-capsular-pigmentation
#14
SeongJin Yun, Seonmi Kang, Kangmoon Seo
A 5-year-old castrated male poodle presented with blindness. Ophthalmic examinations including slit-lamp biomicroscopy, tonometry, ultrasonography, and electroretinography, were performed. The anterior lens capsule of the right eye (OD) was totally pigmented, with persistent pupillary membranes (PPMs). Ultrasonography of the same eye showed severe lens atrophy and retinal detachment. Electroretinography revealed flat a- and b-waves in OD, but normal amplitudes in the left eye (OS). No ocular defects were detected in OS except mature cataract...
May 4, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29731476/prevalence-of-toxoplasma-gondii-and-other-intestinal-parasites-in-cats-in-tokachi-sub-prefecture-japan
#15
Doaa Salman, Wilawan Pumidonming, Eiji Oohashi, Makoto Igarashi
The present study investigated the prevalence of Toxoplasma gondii and other intestinal parasites in cats in the Tokachi subprefecture in Japan. A total of 365 household cats were included in the study, and 353 serum and 351 fecal samples were collected and analyzed. T. gondii IgG antibodies were detected in the sera of 16.14% of cats based on Latex agglutination test and ELISA. For ELISA, T. gondii RH strain tachyzoites lysate and T. gondii SAG2 recombinant protein were used as antigens. Low seropositivity was detected in cats younger than one year and older than 11 years; outdoor and hunter cats showed significantly high seropositivities...
May 2, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29709900/transcriptome-analysis-reveals-differential-gene-expression-in-intramuscular-adipose-tissues-of-jinhua-and-landrace-pigs
#16
Zhiguo Miao, Panpeng Wei, Muhammad Akram Khan, Jinzhou Zhang, Liping Guo, Dongyang Liu, Xiaojian Zhang, Yueyu Bai, Shan Wang
Meat is a rich source of protein, fatty acids and carbohydrates for human needs. In addition to necessary nutrients, high fat contents in pork increase the tenderness and juiciness of the meat, featuring diverse application in various dishes. This study investigated the transcriptomic profiles of intramuscular adipose tissues in Jinhua and Landrace pigs by employing advanced RNA sequencing. Results showed significant interesting to note that there were significant differences in the expression of genes. 1,632 genes showed significant differential expression, 837 genes were up-regulated and 195 genes were down-regulated...
May 1, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29709899/testicular-regulation-of-seasonal-change-in-apocrine-glands-in-the-back-skin-of-the-brown-bear-ursus-arctos
#17
Jumpei Tomiyasu, Daisuke Kondoh, Yojiro Yanagawa, Yoshikazu Sato, Hideyuki Sakamoto, Naoya Matsumoto, Kazuyoshi Sasaki, Shingo Haneda, Motozumi Matsui
Brown bears communicate with other individuals using marking behavior. Bipedal back rubbing has been identified as a common marking posture. Oily substances are secreted via enlarged sebaceous glands in the back skin of male bears during the breeding season. However, whether apocrine gland secretions are associated with seasonal changes remains unknown. The present study aimed to identify histological and histochemical changes in the secretory status and the glycocomposition of the apocrine glands in the back skin of male bears in response to changes in seasons and/or reproductive status...
May 1, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29709903/prevention-the-spread-of-norovirus-infection-on-airborne-route-by-using-plasma-assisted-catalytic-technology-pact-device
#18
Yoshimoto Tanaka, Kan Fujino, Gerald Andrew Larkins, Atsushi Osawa, Yuji Hayashi, Satoshi Taharaguchi
Zoonosis becomes a popular word. Highly pathogenic influenza virus (HPI), Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS) recently occurred at around Africa, Meddle-East and South-East Asia area, whose virus is classified as airborne. Sterilization capability was investigated by using chemical reactor of PACT device. Test on airborne infection was carried out by Feline Calicivirus Vaccine (FCV) strain F9, which is also surrogated human norovirus. It was found that PACT device could sterilize instantly FCV when passing through the plasma space of PACT device...
April 30, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29709902/development-of-a-quantitative-real-time-rt-pcr-assay-for-detecting-taiwan-ferret-badger-rabies-virus-in-ear-tissue-of-ferret-badgers-and-mice
#19
Ai-Ping Hsu, Chun-Hsien Tseng, Yi-Ta Lu, Yu-Hua Shih, Chung-Hsi Chou, Re-Shang Chen, Kuo-Jung Tsai, Wen-Jane Tu, Florence Cliquet, Hsiang-Jung Tsai
In 2013, the first case of Taiwan ferret badger rabies virus (RABV-TWFB) infection was reported in Formosan ferret badgers, and two genetic groups of the virus were distinguished through phylogenetic analysis. To detect RABV-TWFB using a sensitive nucleic acid-based method, a quantitative real-time reverse transcription polymerase chain reaction targeting the conserved region of both genetic groups of RABV-TWFB was developed. This method had a limit of detection (LOD) of 40 RNA copies/reaction and detected viral RNA in brain and ear tissue specimens of infected and dead Formosan ferret badgers and mice with 100% sensitivity and specificity...
April 30, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29695681/investigation-of-genetic-diversity-and-epidemiological-characteristics-of-pasteurella-multocida-isolates-from-poultry-in-southwest-china-by-population-structure-multi-locus-sequence-typing-and-virulence-associated-gene-profile-analysis
#20
Zhangcheng Li, Fangjun Cheng, Shimei Lan, Jianhua Guo, Wei Liu, Xiaoyan Li, Zeli Luo, Manli Zhang, Juan Wu, Yang Shi
Fowl cholera caused by Pasteurella multocida has always been a disease of global importance for poultry production. The aim of this study was to obtain more information about the epidemiology of avian P. multocida infection in southwest China and the genetic characteristics of clinical isolates. P. multocida isolates were characterized by biochemical and molecular-biological methods. The distributions of the capsular serogroups, the phenotypic antimicrobial resistance profiles, lipopolysaccharide (LPS) genotyping and the presence of 19 virulence genes were investigated in 45 isolates of P...
April 25, 2018: Journal of Veterinary Medical Science
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