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Journal of Veterinary Medical Science

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https://www.readbyqxmd.com/read/29445072/molar-eruption-and-identification-of-the-eastern-grey-kangaroo-macropus-giganteus-at-different-ages
#1
Nobuhide Kido, Sohei Tanaka, Yuko Wada, Sumito Sato, Tomoko Omiya
To treat dental disorders and estimate age, it is important that veterinarians understand teeth eruption sequence for the animal in question. Few dental images of the eastern grey kangaroo have been published. In the present study, radiographic imagings of 29 kangaroos, ranging in age from 12 months to 10 years 9 months, was used to surveil the replacement of premolars and the eruption of molars. These images revealed eruption patterns in five stages, while the second and third deciduous premolars were shed non-systematically...
February 15, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29445074/intrapancreatic-accessory-spleen-in-a-harbor-porpoise-phocoena-phocoena
#2
Shotaro Nakagun, Josué Díaz-Delgado, Kátia R Groch, Yoshiyasu Kobayashi
The occurrence of accessory spleens in cetacean species is high yet confirmed reports of intrapancreatic accessory spleen, a congenital malformation, remain undescribed. The current study provides the gross, microscopical, histochemical and immunohistochemical features of an intrapancreatic accessory spleen in a harbor porpoise (Phocoena phocoena). Grossly, a 17 × 18 × 9 mm well-demarcated, dark brown to red mass expanded the left pancreatic lobe. Microscopically, this mass consisted of mature splenic tissue interspersed with exocrine pancreatic acini...
February 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29445073/adiponectin-deficiency-induced-diabetes-increases-tnf%C3%AE-and-ffa-via-downregulation-of-ppar%C3%AE
#3
Haruo Hashimoto, Masafumi Yamamoto, Emika Sugiura, Hayato Abe, Takahiro Kagawa, Motohito Goto, Ri-Ichi Takahashi, Toshio Akimoto, Hiroshi Suemizu
Expression of peroxisome proliferator-activated receptor (PPAR) α was investigated in adiponectin knockout mice to elucidate the relationship between PPARα and adiponectin deficiency-induced diabetes. Adiponectin knockout (Adp -/- ) mice were generated by gene targeting. Glucose tolerance test (GTT), insulin tolerance test (ITT), and organ sampling were performed in Adp -/- mice at the age of 10 weeks. PPARα, insulin, triglyceride, free fatty acid (FFA), and tumor necrosis factor α (TNFα) were analyzed from the sampled organs...
February 14, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29434117/prevalence-and-characteristics-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-domestic-and-imported-chicken-meats-in-japan
#4
Azimun Nahar, Sharda Prasad Awasthi, Noritoshi Hatanaka, Kentaro Okuno, Phuong Hoai Hoang, Jayedul Hassan, Atsushi Hinenoya, Shinji Yamasaki
The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) in retail chicken meats in Japan. Fifty-six domestic and 50 imported (Brazil, n=36; United States, n=8; Thailand, n=6) chicken meat samples were analyzed. The 162 ESBL-Ec included 111 from 43 (77%) domestic samples and 51 from 26 (52%) Brazilian samples. Fifty-three and 30 of 111 and 51 ESBL-Ec from domestic and Brazilian chickens, respectively, were selected for ESBL genotyping...
February 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29434116/bactericidal-and-virucidal-efficacies-of-potassium-monopersulfate-and-its-application-for-inactivating-avian-influenza-virus-on-virus-spiked-clothes
#5
Sorawot Sonthipet, Sakchai Ruenphet, Kazuaki Takehara
An acidic agent, potassium monopersulfate (PMPS), was evaluated for bactericidal and virucidal effects against Salmonella Infantis (SI), Escherichia coli, rifampicin-resistant Salmonella Infantis (SI-rif), Newcastle disease virus (NDV), and avian influenza virus (AIV), in the absence or presence of organic materials. In addition, inactivation activity toward a virus on virus-spiked clothes was also examined. PMPS could inactivate SI, E. coli, and SI-rif even in the presence of organic materials under various concentrations and exposure/contact time conditions...
February 12, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29434093/peripubertal-exposure-to-the-neonicotinoid-pesticide-dinotefuran-affects-dopaminergic-neurons-and-causes-hyperactivity-in-male-mice
#6
Naoki Yoneda, Tadashi Takada, Tetsushi Hirano, Shogo Yanai, Anzu Yamamoto, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Yoshiaki Tabuchi, Nobuhiko Hoshi
Although neonicotinoid pesticides are expected to have harmful influence on mammals, there is little animal experimental data to support the effect and mechanisms. Since acetylcholine causes the release of dopamine, neonicotinoids may confer a risk of developmental disorders via a disturbance in the monoamine systems. Male mice were peripubertally administered dinotefuran (DIN) referring to no observed effect level (NOEL) and performed behavioral and immunohistological analyses. In an open field test, the total locomotor activity was increased in a dose-dependent manner...
February 9, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415902/influence-of-changes-in-the-intestinal-microflora-on-the-immune-function-in-mice
#7
Shigefumi Kishida, Yuko Kato-Mori, Katsuro Hagiwara
The composition of the intestinal microbiota is related to the health and immune function of the host. Administration of antibiotics affects the composition of the intestinal microbiota. However, the effects of immune function on the composition of the intestinal microbiota are still unclear. In this study, we investigated the lymphocyte composition and determined the relationships between lymphocyte function and the intestinal microbiota following antibiotic treatment in mice. To change the composition of the intestinal microbiota, mice were treated with or without antibiotics...
February 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415901/sexual-dimorphism-of-craniomandibular-size-in-the-eurasian-otter-lutra-lutra-from-south-korea
#8
Yung Kun Kim, Eunok Lee, Jin Woo Oh, Sung Yong Han
Sexual size dimorphism of craniomandibular morphology of the Eurasian otter Lutra lutra in South Korea was analyzed using linear measurements. In total, 32 skulls (18 males and 14 females) and 22 linear measurements (16 cranial and 6 mandibular measurements) were used. Our results showed statistically significant sexual dimorphism between male and female skull size. Multivariate analyses using the cranial and mandibular traits showed significant differences between the sexes, respectively. The most dimorphic trait was ectorbital breadth (EOB), and the EOB of the male was approximately 10% greater than that of the female...
February 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415900/identification-and-phase-inversion-of-salmonella-flagellar-antigens-using-immuno-discs
#9
Masatoshi Fujihara, Yukino Tamamura, Hiroyuki Tabuchi, Kaho Uegaki
Disc immuno-immobilization is a simple method for typing the flagellar phase of Salmonellaenterica. We re-examined this method using commercial antisera, which contains the preservative sodium azide. Originally prepared motility agar activates bacterial motility and renders S. enterica resistant to sodium azide, resulting in the formation of immuno-immobilization lines around reactive immuno-discs. Though disc immuno-immobilization serves both serotyping and phase inversion, this method is insufficient for the strains in which phase variation rarely occurs...
February 8, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415922/increased-expression-of-the-stromal-fibroblast-secreted-periostin-in-canine-squamous-cell-carcinomas
#10
Takayuki Mineshige, Kikumi Ogihara, Junichi Kamiie, Go Sugahara, James Ken Chambers, Kazuyuki Uchida, Hiroo Madarame, Kinji Shirota
Canine squamous cell carcinoma (SCC) shows highly invasive and locally destructive growth. In animal models and human cancer cases, periostin plays a critical role in the enhancement of cancer growth; however, the mechanism of involvement in canine cancers remains unknown. The aim of this study was to examine the involvement of periostin in the pathophysiology of SCC in dogs. We examined the localization of periostin and periostin-producing cells in 20 SCC and three squamous papilloma specimens. Furthermore, we focused on transforming growth factor (TGF)-β1, which was assumed to be an inducing factor of periostin, using culture cells...
February 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415921/efficacy-of-mechanical-micro-vibration-in-the-development-of-bovine-embryos-during-in-vitro-maturation-and-culture
#11
Masahiro Takahashi, Tatsutoshi Honda, Shingo Hatoya, Toshio Inaba, Noritoshi Kawate, Hiromichi Tamada
It is currently unclear how mechanical micro-vibration affects the in vitro culture of embryos in Japanese Black cow. In the experimental groups, immature oocytes and fertilized embryos were cultured using the micro-vibration culture system with the vibration set for 5 sec at intervals of 60 min and frequency of 20, 40, or 80 Hz, respectively, during in vitro maturation and in vitro development. Compared with the control group, the rate of blastocyst development significantly increased in the 40 Hz group. In addition, the number of blastocyst cells reduced significantly in the 80 Hz group...
February 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415920/neutralizing-formaldehyde-in-chicken-cadaver-with-urea-and-urea-fertilizer-solution
#12
Le Ngoc Ninh, Sirikachorn Tangkawattana, Peerapol Sukon, Naoki Takahashi, Kazushige Takehana, Prasarn Tangkawattana
This study demonstrated the potential of using urea and urea fertilizer to neutralize formaldehyde (Fd) in chicken cadavers. Initially, in vitro Fd neutralization with various concentrations of urea solution (US) and urea fertilizer solution (UFS) was conducted; subsequently, 18% US and 27% UFS were selected for infusing into the formalinized chickens. The measurement at 48 hr after infusion showed that both solutions could effectively lower Fd in chicken cadavers to below a permissible exposure limit without affecting cadaveric and histological quality...
February 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29415919/quantitative-computed-tomographic-evaluation-of-bone-mineral-density-in-beagle-dogs-comparison-with-dual-energy-x-ray-absorptiometry-as-a-gold-standard
#13
Danbee Kwon, Jaehwan Kim, Hyeeun Lee, Byeol Kim, HyunHee Han, HyunJu Oh, MinJung Kim, Hakyoung Yoon, ByeongChun Lee, Kidong Eom
This study aimed to demonstrate the higher accuracy and reproducibility of quantitative computed tomography (QCT) compared with dual-energy X-ray absorptiometry (DXA) as a gold standard for measuring canine bone mineral density (BMD). Seven middle-aged beagle dogs underwent lumbar vertebral and bilateral femoral DXA and QCT scans. BMD (mg/cm2) was measured at the vertebral body from L2 to L6, femoral neck, and proximal and distal femoral diaphyses. The BMD values were measured 3 times and compared. The BMD value on QCT was higher than that on DXA for femoral BMD but not for vertebral BMD...
February 6, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29398674/canine-case-of-swallowing-syncope-that-improved-after-pacemaker-implantation
#14
Ryuji Fukushima, Toshiki Araie, Naoaki Itou, Takae Kawaguchi, Shusaku Yamada, Aritada Yoshimura, Seijirow Goya, Kazumi Shimada, Tsuyoshi Uchide, Miori Kishimoto, Noboru Machida
A 14-year-old intact male West Highland White Terrier weighing 6.9 kg was admitted to the Tokyo University of Agriculture and Technology Animal Medical Center with the complaint of syncope after showing signs of nausea during feeding. Sinus arrest induced by deglutition was confirmed using a Holter electrocardiography test. However, the clinical symptoms significantly improved after implantation of a permanent pacemaker. Seven months after implantation, the dog died from acute pancreatitis, a cause unrelated to the syncope...
February 5, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29398673/atlantooccipital-overlapping-and-its-effect-on-outcomes-after-ventral-fixation-in-dogs-with-atlantoaxial-instability
#15
Fumitaka Takahashi, Takaharu Hakozaki, Shigenori Kouno, Shuji Suzuki, Asaka Sato, Nobuo Kanno, Yasuji Harada, Shinya Yamaguchi, Yasushi Hara
We compared clinical outcomes after ventral fixation in dogs with atlantoaxial instability (AAI) on the basis of the presence or absence of atlantooccipital overlapping (AOO). Of 41 dogs diagnosed with AAI and treated ventral fixation, 12 exhibited AOO (AOO group), whereas 29 did not (non-AOO group). The AOO group had significantly higher neurological scores before (P=0.024) and 1 month after (P=0.033) surgery compared with the non-AOO group; however, no significant differences were observed between the groups 2 months after surgery...
February 5, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29398671/microbiological-identification-and-analysis-of-waterfowl-livers-collected-from-backyard-farms-in-southern-china
#16
Rongchang Liu, Cuiteng Chen, Yu Huang, Longfei Cheng, Ronghui Lu, Guanghua Fu, Shaohua Shi, Hongmei Chen, Chunhe Wan, Qiuling Fu, Jiansheng Lin
In total, 985 livers were collected from 275 backyard waterfowl farms distributed in seven provinces of southern China. The virus that was most commonly isolated was avian influenza virus, with a 12.1% positivity rate. Of the other positive samples, 10.6% tested positive for avian Tembusu virus, 6.8% for duck hepatitis A virus, 3.8% for duck plague virus, 3.4% for Muscovy duck parvovirus, 3.1% for goose parvovirus, 1.0% for mycoplasma, and 0.9% for respiratory enteric orphan virus. The bacterium that was most commonly isolated was Escherichia coli, with a 47...
February 2, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29398670/the-effect-of-sedation-with-a-combination-of-butorphanol-and-midazolam-on-quantitative-contrast-enhanced-ultrasonography-of-duodenum-in-healthy-dogs
#17
Khoirun Nisa, Sue Yee Lim, Tatsuyuki Osuga, Nozomu Yokoyama, Masahiro Tamura, Noriyuki Nagata, Kazuyoshi Sasaoka, Angkhana Dermlim, Rommaneeya Leela-Arporn, Tomoya Morita, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Hiroshi Ohta, Mitsuyoshi Takiguchi
Quantitative contrast-enhanced ultrasonography (CEUS) enables non-invasive and objective evaluation of intestinal perfusion by quantifying the intensity of enhancement on the intestine after microbubble contrast administration. During CEUS scanning, sedation is sometimes necessary to maintain animal cooperation. Nevertheless, the effect of sedative administration on the canine intestinal CEUS is unknown. This study aimed to investigate the effect of sedation with a combination of butorphanol and midazolam on the duodenal CEUS-derived perfusion parameters of healthy dogs...
February 2, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29386424/phosphodiesterase-9-pde9-regulate-bovine-tracheal-smooth-muscle-relaxation
#18
Tsuyoshi Tajima, Tamami Shinoda, Norimoto Urakawa, Kazumasa Shimizu, Takeharu Kaneda
The present study was designed to clarify phosphodiesterase 9 (PDE9) expression in bovine tracheal smooth muscle tissue, and to elucidate that PDE9 may contribute to the regulation of airway relaxation. PDE9 mRNA expression was detected in bovine tracheal smooth muscle. Sodium nitroprusside (an NO donor) and BAY 73-6691 (a selective PDE9 inhibitor) reduced high K+- and carbachol-induced contraction. BAY 73-6691 relaxed tracheal tissue on the same level with vardenafil (a selective PDE5 inhibitor). These results support our hypothesis that PDE9 plays functional role in the tracheal smooth muscle relaxation...
January 30, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29375089/immunohistochemical-localization-of-inhibin-activin-subunits-in-adult-asian-elephant-elephas-maximus-testes
#19
Sirinart Chaichanathong, Kasuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Apichaya Sudsukh, Nikorn Thongtip
Immunolocalization of inhibin-α and inhibin/activin βA and βB subunits in the testes of Asian elephant was determined. Testicular sections were immunostained with polyclonal antisera against inhibin subunit-α and inhibin/activin βA and βB using the avidin-biotin-peroxidase complex method. Positive immunostaining against inhibin-α subunit was strongly present in Sertoli cells, and positive immunostaining for the inhibin/activin βA and βB subunits was observed in both Sertoli and Leydig cells. These results indicated that while Sertoli cells are the predominant source of inhibin and activin secretions in the testes of adult male Asian elephant, Leydig cells are a source of activin but not inhibin...
January 29, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29375088/minimizing-an-outbreak-of-avian-botulism-clostridium-botulinum-type-c-in-incheon-south-korea
#20
Kidong Son, Yong Kwan Kim, Chanjin Woo, Seung-Jun Wang, Youngsik Kim, Jae-Ku Oem, Weonhwa Jheong, Jipseol Jeong
An outbreak of botulism occurred over a two-month period beginning July 20, 2016. In all, 697 wild birds were found paralyzed or dead at the Namdong reservoir and 11 Gong-gu. Using a mouse bioassay, type C botulinum toxin was identified in the bird serum, liquid cultures of soil samples, and maggot extracts. To minimize further infection of wild birds, we opened the floodgates of the Namdong reservoir adjacent to the Yellow Sea; this decreased the water temperature and the nutrient load such as nitrogen and phosphorus...
January 29, 2018: Journal of Veterinary Medical Science
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