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Cell Proliferation

Hong-Mei Yu, Chen Wang, Zhen Yuan, Guang-Liang Chen, Tao Ye, Bi-Wei Yang
OBJECTIVES: LncRNA nuclear-enriched abundant transcript 1 (NEAT1) participates in the development and progression of multiple malignancies. However, the molecular mechanism by which NEAT1 contributes to colorectal cancer (CRC) remains unclear. METHODS: The association between lncRNA NEAT1 expression and clinicopathological characteristics and prognosis in patients with CRC was analysed by TCGA RNA-sequencing data. MTT, colony formation, flow cytometry, transwell assays and a xenograft tumour model were used to assess the functions of NEAT1...
November 8, 2018: Cell Proliferation
Anne-France de Bengy, Nico Forraz, Louis Danoux, Nicolas Berthelemy, Sébastien Cadau, Olivier Degoul, Valérie Andre, Sabine Pain, Colin McGuckin
OBJECTIVES: Sebaceous glands maintain skin homeostasis by producing sebum. Low production can induce hair loss and fragile skin. Overproduction provokes seborrhoea and may lead to acne and inflammatory events. To better study sebaceous gland maintenance, sebocyte maturation, lipid production and ageing or inflammatory processes, we developed innovative 3D ex vivo models for human sebaceous glands. MATERIALS AND METHODS: Culture conditions and analytical methods optimized on sebocyte monolayers were validated on extracted sebaceous glands and allowed the development of two 3D models: (a) "air-liquid" interface and (b) human fibronectin-coated "sandwich" method...
November 6, 2018: Cell Proliferation
Mubalake Abudoureyimu, Hao Zhou, Yingru Zhi, Ting Wang, Bing Feng, Rui Wang, Xiaoyuan Chu
Exosomes are small membrane vesicles 50-150 nm in diameter released by a variety of cells, which contain miRNAs, mRNAs and proteins with the potential to regulate signalling pathways in recipient cells. Exosomes deliver nucleic acids and proteins to participate in orchestrating cell-cell communication and microenvironment modulation. In this review, we summarize recent progress in our understanding of the role of exosomes in hepatocellular carcinoma (HCC). This review focuses on recent studies on HCC exosomes, considering biogenesis, cargo and their effects on the development and progression of HCC, including chemoresistance, epithelial-mesenchymal transition, angiogenesis, metastasis and immune response...
November 5, 2018: Cell Proliferation
Duanjing Chen, Yuanyuan Gong, Ling Xu, Mengjiao Zhou, Jie Li, Jinlin Song
Through loss- and gain-of-function experiments in knockout and transgenic mice, Forkhead box O (FOXO) family transcription factors have been demonstrated to play essential roles in many biological processes, including cellular proliferation, apoptosis and differentiation. Osteogenic differentiation from mesenchymal stem cells (MSCs) into osteoblasts is a well-organized process that is carefully guided and characterized by various factors, such as runt-related transcription factor 2 (Runx2), β-catenin, osteocalcin (OCN), alkaline phosphatase (ALP) and activating transcription factor 4 (ATF4)...
November 5, 2018: Cell Proliferation
Liang Li, Cui Zhang, Jian-Ling Chen, Fan-Fan Hong, Ping Chen, Jin-Fu Wang
OBJECTIVES: Exposure to microgravity induces many adaptive and pathological changes in human bone marrow mesenchymal stem cells (hBMSCs). However, the underlying mechanisms of these changes are poorly understood. We revealed the gene expression patterns of hBMSCs under normal ground (NG) and simulated microgravity (SMG), which showed an interpretation for these changes by gene regulation and signal pathways analysis. MATERIALS AND METHODS: In this study, hBMSCs were osteogenically induced for 0, 2, 7 and 14 days under normal ground gravity and simulated microgravity, followed by analysis of the differences in transcriptome expression during osteogenic differentiation by RNA sequencing and some experimental verification for these results...
November 5, 2018: Cell Proliferation
Zheng Li, Xingye Li, Xin Chen, Shugang Li, Idy H T Ho, Xiaodong Liu, Matthew T V Chan, William K K Wu
Neuropathic pain, a type of chronic and potentially disabling pain resulting from primary injury/dysfunction of the somatosensory nervous system and spinal cord injury, is one of the most intense types of chronic pain, which incurs a significant economic and public health burden. However, our understanding of its cellular and molecular pathogenesis is still far from complete. Long non-coding RNAs (lncRNAs) are important regulators of gene expression and have recently been characterized as key modulators of neuronal functions...
October 25, 2018: Cell Proliferation
Mengru Ma, Yifei Pei, Xixi Wang, Jiaxin Feng, Yong Zhang, Ming-Qing Gao
OBJECTIVES: The correlations between long non-coding RNAs (lncRNAs) and diverse mammal diseases have been clarified by many researches, but the cognition about bovine mastitis-related lncRNAs remains limited. This study aimed to investigate the potential role of lncRNA X-inactive specific transcript (XIST) in the inflammatory response of bovine mammary epithelial cells. MATERIALS AND METHODS: Two inflammatory bovine mammary alveolar cell-T (MAC-T) models were established by infecting the cells with Escherichia coli (E...
October 25, 2018: Cell Proliferation
Manzhu Zhao, Xiujie Wen, Gang Li, Yingxin Ju, Yingying Wang, Zhi Zhou, Jinlin Song
OBJECTIVE: The aim of this study was to investigate the spatiotemporal expression and potential role of p75NTR in tooth morphogenesis and tissue mineralization. MATERIALS AND METHODS: The dynamic morphology of the four stages (from the beginning of E12.5 d, then E13.5 d and E15.5 d, to the end of E18.5 d) was observed, and the expressions of p75NTR and Runx2 were traced. The ectomesenchymal stem cells (EMSCs) were harvested in vitro, and the biological characteristics were observed...
October 25, 2018: Cell Proliferation
Cancan Zhou, Weikun Qian, Jiguang Ma, Liang Cheng, Zhengdong Jiang, Bin Yan, Jie Li, Wanxing Duan, Liankang Sun, Junyu Cao, Fengfei Wang, Erxi Wu, Zheng Wu, Qingyong Ma, Xuqi Li
OBJECTIVES: Gemcitabine is a standard treatment for advanced pancreatic cancer patients but can cause chemoresistance during treatment. The chemoresistant cells have features of cancer stem cells (CSCs). Resveratrol has been reported to overcome the resistance induced by gemcitabine. However, the mechanism by which resveratrol enhances chemosensitivity remains elusive. Here, we explored the mechanism by which resveratrol enhanced chemosensitivity and the role of sterol regulatory element binding protein 1 (SREBP1) in gemcitabine-induced stemness...
October 19, 2018: Cell Proliferation
Wenjuan Zhang, Yupei Liang, Lihui Li, Xiaofang Wang, Zi Yan, Changsheng Dong, Mu-Sheng Zeng, Qian Zhong, Xue-Kui Liu, Jinha Yu, Shuyang Sun, Xiaojun Liu, Jihui Kang, Hu Zhao, Lak Shin Jeong, Yanmei Zhang, Lijun Jia
OBJECTIVES: The present study aimed to reveal expression status of the neddylation enzymes in HNSCC and to elucidate the anticancer efficacy and the underlying mechanisms of inhibiting neddylation pathway. MATERIALS AND METHODS: The expression levels of neddylation enzymes were estimated by Western blotting in human HNSCC specimens and bioinformatics analysis of the cancer genome atlas (TCGA) database. Cell apoptosis was evaluated by Annexin V fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) stain and fluorescence-activated cell sorting (FACS)...
October 19, 2018: Cell Proliferation
Yanyan Xia, Yuanying Zhang, Mengjiao Shen, Hongpan Xu, Zhiyang Li, Nongyue He
Golgi protein 73 (GP73, also referred to as Golph 2) with 400 amino acids is a 73 kDa transmembrane glycoprotein typically found in the cis-Golg complex. It is primarily expressed in epithelial cells, which has been found upregulated in hepatocytes in patients suffering from both viral and non-viral liver diseases. GP73 has drawn increasing attention for its potential application in the diagnosis of liver diseases such as hepatitis, liver cirrhosis and liver cancer. Herein, we reviewed the discovery history of GP73 and summarized studies by many groups around the world, aiming at understanding its structure, expression, function, detection methods and the relationship between GP73 and liver diseases in various settings...
October 19, 2018: Cell Proliferation
Jingxia Cui, Shuli Man, Nina Cui, Li Yang, Qianbei Guo, Long Ma, Wenyuan Gao
OBJECTIVES: Drug combination has a promising and potential development prospect in the treatment of various cancers. The objective of this study is to investigate the synergistic mechanisms of polyphyllin VII (PVII) and formosanin C (FC) in lung cancer. MATERIALS AND METHODS: The combination of FC and PVII influenced on the apoptosis, autophagy, and the relative signalling pathways were analysed in lung cancer cells. RESULTS: The combination of FC and PVII demonstrated a concentration- dependent growth inhibition in human lung cancer cells...
October 18, 2018: Cell Proliferation
Meng Wang, Yuan Li, Guo-Dong Xiao, Xiao-Qiang Zheng, Ji-Chang Wang, Chong-Wen Xu, Sida Qin, Hong Ren, Shou-Ching Tang, Xin Sun
OBJECTIVES: Breast cancer stem-like cells (BrCSCs) are the major reason for tumour generation, resistance and recurrence. The turbulence of their self-renewal ability could help to constrain the stem cell expansion. The way BrCSCs divided was related to their self-renewal capacity, and the symmetric division contributed to a higher ability. Non-coding long RNA of H19 was involved in multiple malignant procedures; the role and mechanistic proof of non-coding long RNA of H19 in controlling the divisions of BrCSCs were barely known...
October 18, 2018: Cell Proliferation
Kang Zou, Jian Wang, Haiwei Bi, Yabin Zhang, Xueli Tian, Ning Tian, Wanyun Ma, Ji Wu
OBJECTIVES: In vitro differentiation of oocytes from female germline stem cells (FGSCs) has exciting potential applications for reproductive medicine. Some researchers have attempted to reveal the in vitro differentiation capacity of FGSCs. However, no systematic comparative study of in vitro differentiation conditions has been performed for murine FGSCs (mFGSCs). MATERIALS AND METHODS: mFGSCs line was cultured under five different conditions for in vitro differentiation...
October 17, 2018: Cell Proliferation
Lufang Zhang, Lushan Wang, Dong Dong, Zhiyong Wang, Wei Ji, Man Yu, Fei Zhang, Ruifang Niu, Yunli Zhou
OBJECTIVES: MiR-34 is a tumour suppressor in breast cancer. Neurokinin-1 receptor (NK1R), which is the predicted target of the miR-34 family, is overexpressed in many cancers. This study investigated the correlation and clinical significance of miR-34 and NK1R in breast cancer. MATERIALS AND METHODS: Western blotting, quantitative reverse transcription-PCR (qRT-PCR) and luciferase assays were conducted to analyse the regulation of NK1R by miR-34 in MDA-MB-231, MCF-7, T47D, SK-BR-3 and HEK-293 T cells...
October 17, 2018: Cell Proliferation
Gaoming Li, Xiongbo Song, Rui Li, Li Sun, Xiaoyuan Gong, Cheng Chen, Liu Yang
OBJECTIVES: To investigate the role of zyxin-involved actin regulation in expression level of vinculin focal adhesion and collagen production of chondrocyte and its possible underlying mechanism. MATERIALS AND METHODS: Chondrocytes obtained from rabbit articular cartilage were used in this study. The expression of zyxin, actin and vinculin, as well as the extracellular matrix (ECM) protein collagen type I, II and X (COL I, II and X) of chondrocytes were compared between zyxin-knockdown group and negative control group, and between transforming growth factor-β1 (TGF-β1) treatment group and non-treatment group, respectively...
October 17, 2018: Cell Proliferation
Zhiwei Ma, Ping Li, Xingjie Hu, Haiyun Song
OBJECTIVES: Over the past decade an intriguing connection between cell polarity and tumorigenesis has emerged. Multiple core components of the junction complexes that help to form and maintain cell polarity display both pro- and anti-tumorigenic functions in a context-dependent manner, with the underlying mechanisms poorly understood. MATERIALS AND METHODS: With transgenic fly lines that overexpress or knock down specific signalling components, we perform genetic analysis to investigate the precise role of the polarity protein Canoe (Cno) in tumorigenesis and the downstream pathways...
October 17, 2018: Cell Proliferation
Mengting Liu, Wenjuan Ma, Qianshun Li, Dan Zhao, Xiaoru Shao, Qian Huang, Liying Hao, Yunfeng Lin
OBJECTIVES: Aptamer sgc8c is a short DNA sequence that can target protein tyrosine kinase 7 (PTK7), which was overexpressed on many tumour cells. This study aimed to fabricate a novelty DNA nanostructure drug delivery system target on PTK7-positive cells-CCRF-CEM (human T-cell ALL). METHODS: Aptamer-modified tetrahedron DNA was synthesized through one-step thermal annealing process. The sgc8c-TDNs (s-TDNs) loading DOX complexes were applied to investigate the effect to PTK7-negative and -positive cells...
October 12, 2018: Cell Proliferation
Anbang He, Shiming He, Xuesong Li, Liqun Zhou
Long noncoding RNAs (lncRNAs) are a class of noncoding, endogenous, single-stranded RNAs longer than 200 nucleotides in length that are transcribed by RNA polymerase II. Mounting evidence has indicated that lncRNAs play key roles in several physiological and pathological processes by modifying gene expression at the transcriptional, posttranscriptional, epigenetic, and translation levels. Many reports have demonstrated that lncRNAs function as potential oncogene or tumour suppressors and thus play vital regulatory roles in tumourigenesis and tumour progression...
October 5, 2018: Cell Proliferation
Meizhu Xiao, Ying Feng, Chongdong Liu, Zhenyu Zhang
Cancers have been a worldwide health problem with a high mortality rate, but ideal biomarkers are not available to effectively screen and diagnose patients. Currently, an increasing number of long noncoding RNAs have been reported to be abnormally expressed in human carcinomas and play a vital role in tumourigenesis. Plasmacytoma variant translocation 1 (PVT1) is upregulated in various carcinomas, and its overexpression is associated with poor survival in cancer patients. We conduct an updated meta-analysis to determine its potential in prognosis for tumours...
September 5, 2018: Cell Proliferation
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