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Cell Proliferation

Limei Wang, Jin Zhang, Cunwei Wang, Yuping Qi, Mi Du, Wenhua Liu, Chengzhe Yang, Pishan Yang
OBJECTIVES: Low concentrations of tumour necrosis factor-alpha (TNF-α) have been reported to promote osteogenic differentiation. In this study, a series of in vitro experiments was performed to investigate underlying molecular mechanisms involved. MATERIALS AND METHODS: MC3T3-E1 murine preosteoblasts were treated with TNF-α at doses of 0, 0.1 or 1 ng/mL. The ephrinB2-EphB4 signalling pathway was activated using ephrinB2-fc, or inhibited using lentiviruses encoding siRNAs specifically targeting EphB4...
October 11, 2016: Cell Proliferation
Zhixun Yin, Hongmei Ding, Erxing He, Jingchen Chen, Ming Li
BACKGROUND AND OBJECTIVES: MicroRNAs are small non-coding RNAs involved in pathogenesis and progression of human malignancies. MicroRNA-491-5p (miR-491-5p) is down-regulated in many human cancers where it would serve as a tumour suppressor. However, the role played by miR-491-5p in pathogenesis of human osteosarcoma has remained largely unknown. This study has been conducted to examine effects of miR-491-5p on migration and proliferation of cells of the SAOS-2 and MG63 osteosarcoma lines, and mechanisms of those effects...
October 5, 2016: Cell Proliferation
Yi Zhang, Sirong He, Xiaojion Du, Yaowen Jiang, Bole Tian, Shuyun Xu
OBJECTIVES: Increasing evidences indicate that microRNAs may play a critical role in the regulation of hypoxia/reoxygenation (H/R) injury, and their expression is associated with mTORC activity. We propose that rapamycin modulates H/R-induced islets injury by regulating microRNA expression. MATERIALS AND METHODS: We investigated whether rapamycin treatment could alter the expression profile of miRNAs in islets. Furthermore, we assessed the islet apoptosis and function after H/R or syngeneic islet transplantation...
September 29, 2016: Cell Proliferation
Yang Yu, Yuan Yin, Rui-Xin Wu, Xiao-Tao He, Xi-Yu Zhang, Fa-Ming Chen
OBJECTIVES: Cell migration is necessary for numerous physiological cell processes. Although either inflammatory or hypoxic stimuli of certain dose and duration have positive influence on cell migration, their combination has not been shown to result in a synergistic effect. MATERIALS AND METHODS: In this study, we investigated combined effects of hypoxia and low-dose inflammatory stimulus (one-tenth of that of a previously used concentration) on migration of human bone marrow-derived mesenchymal stem cells (BMMSCs)...
September 28, 2016: Cell Proliferation
Kun Yang, Yingying Wang, Yingxin Ju, Gang Li, Chang Liu, Junyu Liu, Qi Liu, Xiujie Wen, Lu Chuan Liu
OBJECTIVES: The aim of this study was to investigate whether p75NTR (p75 neurotrophin receptor) regulates differential mineralization capacity of rEMSCs (rat ectomesenchymal stem cells) and underlying mechanisms associated with Mage-D1 (melanoma-associated antigens-D1). MATERIALS AND METHODS: Immunohistochemical staining of p75NTR in developing tooth germs was performed on E12.5d (embryonic 12.5 days) and E19.5d (embryonic 19.5 days). E12.5d EMSCs and E19.5d EMSCs were isolated in the same pregnant Sprague-Dawley rats from embryonic maxillofacial processes and tooth germs...
September 27, 2016: Cell Proliferation
L Farahmand, B Darvishi, K Majidzadeh-A, A Madjid Ansari
Despite numerous remarkable achievements in the field of anti-cancer therapy, tumour relapse and metastasis still remain major obstacles in improvement of overall cancer survival, which may be at least partially owing to epithelial-mesenchymal transition (EMT). Multiple signalling pathways have been identified in EMT; however, it appears that the role of the Hedgehog and WNT/β-catenin pathways are more prominent than others. These are well-known preserved intracellular regulatory pathways of different cellular functions including proliferation, survival, adhesion and differentiation...
September 27, 2016: Cell Proliferation
Changyue Xue, Jiamin Xie, Dan Zhao, Shiyu Lin, Tengfei Zhou, Sirong Shi, Xiaoru Shao, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: The aim of the study was to investigate the role of the JAK/STAT3 signalling pathway in angiogenesis. MATERIALS AND METHODS: The model established in vitro, involved a 3D collagen gel being implanted with endothelial cells (ECs) from red fluorescent protein-labelled mice, and adipose-derived stromal cells (ASCs) from green fluorescent protein-labelled mice. Phenomena of angiogenesis, after treatment by the inhibitor and the activator of JAK/STAT3 pathway respectively, were observed using confocal laser scanning microscopy...
September 25, 2016: Cell Proliferation
Didi Wu, Dahai Yu, Xiuxia Wang, Bingzhi Yu
: In mouse fertilized eggs, correct assembly and distribution of the actin cytoskeleton are intimately related to cleavage in early-stage embryos. However, in mouse fertilized eggs, mechanisms and involved factors responsible for regulating the actin cytoskeleton are poorly defined. In this study, mTORC2, PKB/Akt and Girdin were found to modulate division of mouse fertilized eggs by regulating distribution of the actin cytoskeleton. RNA interference (RNAi)-mediated depletion of mTORC2, Akt1 or Girdin disrupted F-actin rearrangement and strongly inhibited egg development...
September 25, 2016: Cell Proliferation
Yannan Chen, Xia Wang, Chengwei Duan, Jie Chen, Ming Su, Yunfeng Jin, Yan Deng, Di Wang, Caiwen Chen, Linsen Zhou, Jialin Cheng, Wei Wang, Qinghua Xi
Ovarian cancer is a leading cause of death among gynaecologic malignancies. Despite many years of research, it still remains sparing in reliable diagnostic markers and methods for early detection and screening. Transforming growth factor β-activated protein kinase 1 (TAK1)-binding protein 3 (TAB3) was initially characterized as an adapter protein essential for TAK1 activation in response to IL-1β or TNFα, however, the physiological role of TAB3 in ovarian cancer tumorigenesis is still not fully understood...
September 21, 2016: Cell Proliferation
Na Fu, Jinfeng Liao, Shiyu Lin, Ke Sun, Taoran Tian, Bofeng Zhu, Yunfeng Lin
OBJECTIVE: Management of chondral defects has long been a challenge due to poor self-healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized combination of novel scaffolds, cell sources and growth factors, has emerged as a promising strategy for cartilage regeneration and repair. In this study, the aim was to investigate the role of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) PCEC scaffold in cartilage repair...
September 19, 2016: Cell Proliferation
Meng Wu, Jianfang Lou, Shuping Zhang, Xian Chen, Lei Huang, Ruihong Sun, Peijun Huang, Shiyang Pan, Fang Wang
OBJECTIVES: The aim of this study was to investigate a possible mechanism of CD8(+) regulatory T-cell (Treg) production in an ovarian cancer (OC) microenvironment. MATERIALS AND METHODS: Agilent microarray was used to detect changes in gene expression between CD8(+) T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line. QRT-PCR was performed to determine glycolysis gene expression in CD8(+) T cells from a transwell culturing system and OC patients...
September 19, 2016: Cell Proliferation
S Balakrishnan, F A Bhat, P Raja Singh, S Mukherjee, P Elumalai, S Das, C R Patra, J Arunakaran
OBJECTIVES: Epidermal growth factor plays a critical role in breast malignancies by enhancing cell proliferation, invasion, angiogenesis and metastasis. Epithelial-mesenchymal transition (EMT) is a crucial process by which epithelial cells lose polarity and acquire migratory mesenchymal properties. Gold nanoparticles are an efficient drug delivery vehicle for carrying chemotherapeutic agents to target cancer cells and quercetin is an anti-oxidative flavonoid known with potent anti-malignant cell activity...
September 18, 2016: Cell Proliferation
Yuan Yue, Xingmei Yang, Liang Zhang, Xun Xiao, Neel R Nabar, Yunfeng Lin, Liang Hao, Dongjiao Zhang, Jingyi Huo, Jingle Li, Xiaoxiao Cai, Min Wang
OBJECTIVES: Peripheral nerve injuries are a common occurrence, resulting in considerable patient suffering; it also represents a major economic burden on society. To improve treatment options following peripheral nerve injuries, scientists aim to find a way to promote Schwann cell (SC) myelination to help nerves to carry out their functions effectively. In this study, we investigated myelination ability of SCs, regulated by co-culture with adipose-derived stem cells (ASCs) or low-intensity pulsed ultrasound (LIPUS), and synergistic effects of combined treatments...
September 14, 2016: Cell Proliferation
Yuyin Li, Ailong Guo, Yajuan Feng, Yueying Zhang, Jianjun Wang, Lifang Jing, Yali Yan, Lei Jing, Zhenxing Liu, Long Ma, Aipo Diao
OBJECTIVES: TMEPAI (transmembrane prostate androgen-induced protein) has been reported to be overexpressed during tumour progression; however, little is known concerning transcriptional mechanisms regulating TMEPAI gene expression. MATERIALS AND METHODS: In this study, the TMEPAI gene promoter has been identified and characterized, and the effects of Sp1 on TMEPAI-induced viability of A549 cells were evaluated, using MTT and colony formation assays. RESULTS: We found that the sequence between -298 and +24 consists of basal promoter activity for TMEPAI...
September 14, 2016: Cell Proliferation
Yanhui Bai, Weiqun Wang, Guangli Sun, Mingchang Zhang, Jingmin Dong
OBJECTIVE: Curcumin (capable of inhibiting angiogenic growth of human umbilical vein endothelial cells [HUVECs]), can be employed in vitro as a model of pathogenesis of corneal neovascularization (CRNV). The aim of this study was to explore regulatory mechanisms of microRNA (miR) levels after curcumin treatment. MATERIALS AND METHODS: Expression profiles of miRs in curcumin-treated HUVECs were investigated by miR microassay. Specific mimics and inhibitors of miR-1275 or miR-1246 were transfected into HUVECs...
September 14, 2016: Cell Proliferation
P Gorjala, J G Cairncross, R K Gary
OBJECTIVES: Beryllium salts (here, beryllium sulphate) can produce a cytostatic effect in some cell types. The basis for this effect may include increased expression of proliferation inhibitors, reduced expression of proliferation promoters, or both. This study sought to determine the role of p53, the tumour-suppressing transcription factor, in mediating beryllium-induced cytostasis. MATERIALS AND METHODS: Human A172 glioma cells express wild-type TP53 gene. Activity of p53 was experimentally manipulated using siRNA and related approaches...
September 9, 2016: Cell Proliferation
Lanying Sun, Jin Liang, Qibao Wang, Zhaoyuan Li, Yi Du, Xin Xu
OBJECTIVES: Tongue squamous cell carcinoma (TSCC) is the most frequent type of oral malignancy. Increasing evidence has shown that miRNAs play key roles in many biological processes such as cell development, invasion, proliferation, differentiation, metabolism, apoptosis and migration. MATERIALS AND METHODS: qRT-PCR analysis was performed to measure miR-137 expression. CCK-8 analysis, cell colony formation, wound-healing analysis and invasion were performed to detect resultant cell functions...
October 2016: Cell Proliferation
Yunchuan Sun, Jianyong Zhao, Xiaoming Yin, Xiangkun Yuan, Jianfei Guo, Jianqiang Bi
OBJECTIVES: Emerging studies have demonstrated that microRNAs (miRNAs) play crucial roles in carcinogenesis of many developing human tumours. However, the functions and mechanisms of miR-297 in lung cancer have, up to now, been largely undefined. MATERIALS AND METHODS: Here, miR-297 expression was measured in lung adenocarcinoma tissues and cell lines, using qRT-PCR. Lung adenocarcinoma cell line was treated with an miR-297 mimic. MTT and colony analysis were performed to detect cell proliferation and colony formation...
October 2016: Cell Proliferation
Qimin Wang, Guifeng Li, Baolin Li, Qiu Chen, Dongdong Lv, Jiaying Liu, Jieyu Ma, Nai Sun, Longqiu Yang, Xuejie Fei, Qiong Song
Sevoflurane is a frequently-used clinical inhalational anaesthetic and can cause toxicity to embryos during foetal development. Embryonic stem cells (ESCs) are derived from the inner cell mass of blastospheres and can be used as a useful model of early development. Here, we found that sevoflurane significantly influenced self-renewal ability of mESCs on stemness maintenance and cell proliferation. The cell cycle was arrested via G1 phase delay. We further found that sevoflurane upregulated expression of miR-7a,7b to repress self-renewal...
October 2016: Cell Proliferation
Lingqian Du, Ruijuan Feng, Shaohua Ge
OBJECTIVES: Stromal cell-derived factor-1α (SDF-1α) plays an important role in tissue regeneration in various tissues including the periodontium. A potential limitation for its use derives from its sensitivity to cleavage by dipeptidyl peptidase-IV (DPP-IV). Parathyroid hormone (PTH) reduces enzymatic activity of DPP-IV and is suggested to be a promising agent for periodontal tissue repair. The purpose of this study was to provide insight into how SDF-1α and intermittent PTH treatment might affect proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) in vitro...
October 2016: Cell Proliferation
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