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Cell Proliferation

Zhikun Li, Yi Wang, Ruixi Hu, Ruijun Xu, Wei Xu
OBJECTIVES: This study aims to reveal the roles and related mechanisms of LncRNA B4GALT1-AS1 in osteosarcoma (OS) cells stemness and migration. MATERIALS AND METHODS: Real-time quantitative PCR (RT-qPCR) was used to detect the expression of several LncRNAs in OS tissues and normal adjacent tissues and in OS mammospheres and cells. Cell viability, transwell migration, tumour spheres formation and in vivo tumour formation assays were used to examine the effects of LncRNA B4GALT1-AS1 on OS progression...
September 4, 2018: Cell Proliferation
Jianzhong Zhang, Huayong Hu, Yaoxin Zhao, Yulin Zhao
OBJECTIVES: To investigate the roles played by the circular RNA (circRNA) molecule ciRS-7 (CDR1as) and tumour suppressor miRNA-7 (miR-7) in laryngeal squamous cell carcinoma (LSCC). METHODS: Specimens of LSCC tissue (n = 30) and corresponding relative normal tissue (n = 30) were collected to determine their levels and clinical significance of CDR1as/mir-7 expression. The CDR1as and miR-7 were overexpressed in LSCC cells to investigate its function and mechanism in vitro and in vivo...
September 4, 2018: Cell Proliferation
Maohuan Lin, Woliang Yuan, Zizhuo Su, Caina Lin, Tucheng Huang, Yangxin Chen, Jingfeng Wang
OBJECTIVES: Yes-associated protein (YAP) has been reported to regulate cell proliferation and differentiation. We aimed to characterize the role of YAP in angiotensin II (Ang II)-induced hypertensive vascular remodelling (HVR) and vascular smooth muscle cells (VSMCs) phenotypic modulation and to explore the underlying mechanisms. MATERIALS AND METHODS: An HVR rat model was established by continuous Ang II infusion for 2 weeks. Western blotting, qRT-PCR, and confocal microscopy were conducted to assess YAP expression...
August 29, 2018: Cell Proliferation
Ruida Xu, Fan Feng, Xiaosheng Yu, Zude Liu, Lifeng Lao
OBJECTIVE: Accumulating data show that dysregulation of long noncoding RNAs (lncRNAs) acts a critical role in a variety of malignancies. Among these lncRNAs, small nucleolar RNA host genes (SNHGs) are associated with tumour growth and progression. But, the molecular mechanisms by which SNHG4 contributes to osteosarcoma remain undocumented. METHODS: The association between lncRNA SNHG4 expression and clinicopathologic characteristics and prognosis in patients with osteosarcoma was analysed by TCGA RNA-sequencing data...
August 28, 2018: Cell Proliferation
Chuan Cao, Wei Huang, Nan Zhang, Fengbo Wu, Ting Xu, Xiaoli Pan, Cheng Peng, Bo Han
OBJECTIVES: Autophagy and apoptosis are major types of eukaryotic programmed cell death, and regulating these processes holds promise for treating cancers. In this study, we explored the regulation mechanisms of narciclasine to autophagy and apoptosis processes in triple-negative breast cancer. MATERIALS AND METHODS: Effects of narciclasine on proliferation, apoptosis, and autophagy of HCC-1937 and MDA-MB-231 triple-negative breast cancer (TNBC) cells were assessed using transmission electronic microscopy, flow cytometry following staining with Annexin V-FITC and propidium iodide, RNA sequencing, real-time PCR, and Western blotting...
August 28, 2018: Cell Proliferation
Sangkyu Park, Hyewon Kim, Kichul Kim, Sangho Roh
OBJECTIVES: The sonic hedgehog (Shh) signalling pathway has an important role in the maintenance of various stem cells and organogenesis during development. However, the effect of Shh in skin-derived precursors (SKPs), which have the capacity for multipotency and self-renewal, is not yet clear. The present study investigated the effects of the Shh signalling pathway on the proliferation and self-renewal of murine SKPs (mSKPs). METHODS: The Shh signalling pathway was activated by treatment with purmorphamine (Shh agonist) or recombinant Shh in mSKPs...
August 27, 2018: Cell Proliferation
Zhiyuan Zhang, Jie Li, Yuanjian Huang, Wen Peng, Wenwei Qian, Jiou Gu, Qingyuan Wang, Tao Hu, Dongjian Ji, Bing Ji, Yue Zhang, Shijia Wang, Yueming Sun
OBJECTIVES: MicroRNAs (miRNAs) as small noncoding RNA molecules function by regulating their target genes negatively. MiR-1258 was widely researched in multicancers, but its role remains unclear in colorectal cancer (CRC). METHODS: The expression of miR-1258 and its specific target gene were detected in human CRC specimens and cell lines by miRNA RT-PCR, qRT-PCR and Western blot. The effects of miR-1258 on CRC proliferation were evaluated using CCK-8 assays, EdU incorporation, colony formation assays and cell-cycle assays; in vitro and the in vivo effects were investigated using a mouse tumorigenicity model...
August 24, 2018: Cell Proliferation
Yuedi Yang, Zhongbing Huang, Ximing Pu, Guangfu Yin, Lei Wang, Fabao Gao
OBJECTIVE: Five-year survival rate at early lung tumour was about 70%; however, its early diagnosis rate was still at a low level, so the enhancement of diagnosis level for early lung tumour is the key factor to increase the survival rate. Diagnosis and therapy of early lung tumour are still challenged. METHODS: The magnetic nanochains (NCs) with biocompatibility and transverse relaxivity (r2  = 231 Fe mmol l-1  s-1 ) were fabricated through a co-precipitation method in the assistance of dextran, and then, linked with chlorotoxin (CTX) and curcumin (Cur) via the PEGylation and carbodiimide technique (named as CTX-NCs-Cur)...
August 21, 2018: Cell Proliferation
Yuanyuan Li, Minyue Bao, Chunlan Yang, Jiao Chen, Shu Zhou, Rong Sun, Chuanfang Wu, Xin Li, Jinku Bao
OBJECTIVES: The aim of this study was to obtain antitumour molecules targeting to activate PKM2 through adequate computational methods combined with biological activity experiments. METHODS: The structure-based virtual screening was utilized to screen effective activator targeting PKM2 from ZINC database. Molecular dynamics simulations were performed to evaluate the stability of the small molecule-binding PKM2 complex systems. Then, cell survival experiments, glutaraldehyde crosslinking reaction, western blot, and qPCR experiments were used to detect the effects of top hits on various cancer cells and the targeting specificity of PKM2...
August 21, 2018: Cell Proliferation
Xin Zhou, Ran Yu, Yanlin Long, Jiajia Zhao, Shaoling Yu, Qingming Tang, Lili Chen
OBJECTIVES: Skeletal mandibular hypoplasia (SMH), a common type of developmental deformities, results in impaired aesthetics of facial profile, occlusal dysfunction and poor life quality. In this study, BMAL1 deficiency leads to SMH formation, and we aim to investigate the mechanism by which BMAL1 deficiency induces SMH. MATERIALS AND METHODS: Circadian rhythm-disordered mouse models were constructed by placing animals in a jet lag schedule of 6-h light advance every 7 days for 4 or 8 weeks...
August 17, 2018: Cell Proliferation
I Calejo, Raquel Costa-Almeida, Ana Isabel Gonçalves, Dominika Berdecka, Rui Luis Reis, Manuela Estima Gomes
OBJECTIVES: This work aimed at studying in vitro interactions between human tendon-derived cells (hTDCs) and pre-osteoblasts (pre-OBs) that may trigger a cascade of events involved in enthesis regeneration. MATERIALS AND METHODS: The effect of 5 osteogenic medium (OM) conditions over the modulation of hTDCs and pre-OBs towards the tenogenic and osteogenic phenotypes, respectively, was studied. Three different medium conditions were chosen for subsequently establishing a direct co-culture system in order to study the expression of bone, tendon and interface-related markers...
August 14, 2018: Cell Proliferation
Yue Pan, Yongjin Wu, Jialei Hu, Yujia Shan, Jia Ma, Huipeng Ma, Xia Qi, Li Jia
OBJECTIVES: Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR and to explore underlying mechanism involved in HOTAIR/miR-124/alpha-2, 8-sialyltransferase 4 (ST8SIA4) axis-regulated progression in RCC. MATERIALS AND METHODS: Real-time PCR analyses and western blots were performed to the levels of HOTAIR, miR-124 and ST8SIA4 expression in human RCC tissues and RCC cell lines (ACHN and 786-O)...
August 13, 2018: Cell Proliferation
Jianan Chen, Yan Yu, Xiaolong Chen, Yuting He, Qiuyue Hu, Hongqiang Li, Qicai Han, Fang Ren, Juan Li, Chao Li, Jie Bao, Zhigang Ren, Zhenfeng Duan, Guangying Cui, Ranran Sun
OBJECTIVES: Gallbladder carcinoma (GBC) is the most highly aggressive cancer of biliary tract, but effective therapeutics are lacking. Emerging evidence has unveiled that miR-139-5p is aberrantly downregulated in cancers, including GBC. However, the functions and mechanisms of miR-139-5p in GBC remain unclear. MATERIALS AND METHODS: MiR-139-5p-overexpression was established in GBC cell lines, after which cell proliferation, migration, invasion, colony formation, and glucose metabolism were assayed in vitro...
August 13, 2018: Cell Proliferation
Guodong Xiao, Yuan Li, Meng Wang, Xiang Li, Sida Qin, Xin Sun, Rui Liang, Boxiang Zhang, Ning Du, Chongwen Xu, Hong Ren, Dapeng Liu
OBJECTIVES: FBXW7 acts as a tumour suppressor by targeting at various oncoproteins for ubiquitin-mediated degradation. However, the clinical significance and the involving regulatory mechanisms of FBXW7 manipulation of NSCLC regeneration and therapy response are not clear. MATERIALS AND METHODS: Immunohistochemical staining and qRT-PCR were applied to detect FBXW7 and Snai1 expression in 100 samples of NSCLC and matched tumour-adjacent tissues. FBXW7 manipulation of cancer biological functions were studied by using MTT assay, immunoblotting, flow cytometry, transwells, wound healing assay, and sphere-formation assays...
August 9, 2018: Cell Proliferation
Xin Yu, Heyi Zheng, Gary Tse, Lin Zhang, William Ka Kei Wu
Deregulation of long noncoding RNAs (lncRNAs) has been implicated in tumourigenesis. Cancer Susceptibility Candidate 2 (CASC2) is a lncRNA downregulated in multiple cancer types, including endometrial, lung, gastric and colorectal cancers. CASC2 functions as a tumour-suppressive lncRNA though multiple mechanisms, such as sequestration of oncogenic microRNAs and repression of Wnt/β-catenin signalling. Pertinent to clinical practice, the use of CASC2 as a prognostic marker has been demonstrated in sporadic studies...
August 9, 2018: Cell Proliferation
Wenjuan Ma, Xueping Xie, Xiaoru Shao, Yuxin Zhang, Chenchen Mao, Yuxi Zhan, Dan Zhao, Mengting Liu, Qianshun Li, Yunfeng Lin
OBJECTIVES: The main purpose of current study was to explore the effects of tetrahedral DNA nanostructures (TDNs) on neuroectodermal (NE-4C) stem cells migration and unveil the potential mechanisms. MATERIALS AND METHODS: The successfully self-assembled TDNs were also determined by dynamic light scattering (DLS). A bidirectional wound-healing assay and transwell chamber assay were employed to test the migrating behaviour of NE-4C stem cells cultured under different conditions...
August 9, 2018: Cell Proliferation
Lingling Shang, Ting Wang, Dongdong Tong, Wenyan Kang, Qianyu Liang, Shaohua Ge
OBJECTIVES: Prolyl hydroxylases (PHDs) play essential roles in oxygen-sensing system, whereas the effects of PHDs on inflammation have not been totally uncovered. Our study aimed to investigate the role of PHDs in lipopolysaccharide (LPS)-induced inflammation of human gingival fibroblasts (HGFs) and clarify the potential mechanisms. MATERIALS AND METHODS: A pan hydroxylase inhibitor, dimethyloxallyl glycine (DMOG), and RNA interference were used to explore the role of PHDs in inflammation...
August 9, 2018: Cell Proliferation
A Arun, M I Ansari, P Popli, S Jaiswal, A K Mishra, A Dwivedi, K Hajela, R Konwar
OBJECTIVES: In our ongoing studies to develop ER targeting agents, we screened for dual-acting molecules with a hypothesis that a single molecule can also target both ER positive and negative groups of breast cancer. MATERIALS AND METHODS: 1-(2-(4-(Dibenzo[b,f]thiepin-10-yl)phenoxy)ethyl)piperidine (DTPEP) was synthesized and screened in both MCF-7 (ER+ve) and MDA-MB-231 (ER-ve) cells. Assays for analysis of cell cycle, ROS, apoptosis and MMP loss were carried out using flow cytometry...
August 9, 2018: Cell Proliferation
Nan Chen, Zhibing Ou, Wenfeng Zhang, Xiwen Zhu, Peizhi Li, Jianping Gong
OBJECTIVES: The non-canonical inflammasome pathway was described which engages caspase-11 to mediate pyroptosis and the subsequent release of IL-1α, IL-1β and IL-18 in TLR4-independent way. Cathepsin B is capable of activating caspase-11 under cell-free conditions which may regulate non-canonical NLRP3 inflammasome pathway. In this study, we aimed to further investigate cathepsin B as potential activators of proinflammatory caspases which may be released upon proinflammatory stimuli and regulate non-canonical NLRP3 inflammasome pathway by modulating the activity of caspase-11...
August 7, 2018: Cell Proliferation
Ruixi Hu, Xiaodong Zhu, Chao Chen, Ruijun Xu, Yifan Li, Wei Xu
OBJECTIVES: This work aims to reveal the roles and related mechanisms of RNA binding protein PUM2 in osteosarcoma progression. MATERIALS AND METHODS: Transcriptome analysis based on RNA sequencing data, real-time quantitative PCR (RT-qPCR), and western blot analysis were used to detect the expression of RBPs and miRNAs in OS and normal adjacent tissues, and the correlation between them in OS tissues. RT-qPCR, western blot, cell viability, transwell migration, tumour spheres formation and in vivo tumour formation assays were used to examine the effects of RBP PUM2 on OS progression...
August 7, 2018: Cell Proliferation
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