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Cell Proliferation

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https://www.readbyqxmd.com/read/28682508/bone-marrow-endothelial-progenitor-cells-activate-hepatic-stellate-cells-and-aggravate-carbon-tetrachloride-induced-liver-fibrosis-in-mice-via-paracrine-factors
#1
Manali Garg, Savneet Kaur, Arpita Banik, Vikash Kumar, Archana Rastogi, Shiv K Sarin, Asok Mukhopadhyay, Nirupma Trehanpati
OBJECTIVES: Bone marrow derived endothelial progenitor cells (BM-EPCs) are increased in chronic liver disease (CLD). Their role in hepatic fibrosis and regeneration remains an area of intense studies. We investigated the migration and secretory functions of BM-EPCs in fibrotic mice liver. MATERIALS AND METHODS: Bone marrow cells from C57BL6-GFP mice were transplanted into the femur of irradiated C57BL6 mice, followed by CCl4 doses for 8 weeks, to develop hepatic fibrosis (n = 36)...
July 6, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28682474/autologous-serum-supplement-favours-in-vitro-regenerative-paracrine-factors-synthesis
#2
Nazmul Haque, Noor Hayaty Abu Kasim, Noor Lide Abu Kassim, Mohammad Tariqur Rahman
OBJECTIVES: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. MATERIALS AND METHODS: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated...
July 6, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28618452/murine-melanoma-cells-incomplete-reprogramming-using-non-viral-vector
#3
D A D Câmara, A S Porcacchia, A S Costa, R A Azevedo, I Kerkis
OBJECTIVES: The reprogramming of cancer cells into induced pluripotent stem cells or less aggressive cancer cells can provide a modern platform to study cancer-related genes and their interactions with cell environment before and after reprogramming. Herein, we aimed to investigate the reprogramming capacity of murine melanoma B16F10 cells. MATERIALS AND METHODS: The B16F10 was transfected using non-viral circular DNA plasmid containing the genes Sox-2, Oct4, Nanog, Lin28 and green fluorescent protein (GFP)...
June 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28618450/sav1-represses-the-development-of-human-colorectal-cancer-by-regulating-the-akt-mtor-pathway-in-a-yap-dependent-manner
#4
Jianwu Jiang, Wei Chang, Yang Fu, Yongshun Gao, Chunlin Zhao, Xiefu Zhang, Shuijun Zhang
OBJECTIVES: SAV1 is a human homologue of Salvador that contains two protein-protein interaction modules known as WW domains and acts as a scaffolding protein. SAV1 participates in the development of diverse types of cancer. We aimed to investigate the role of SAV1 in human colorectal cancer. MATERIALS AND METHODS: Human colorectal cancer samples were used to study the expression of SAV1 and YAP. Loss-of-function and gain-of-function strategies were used to study the effects of SAV1 on colorectal cancer cell growth...
June 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28597490/downregulation-of-usp32-inhibits-cell-proliferation-migration-and-invasion-in-human-small-cell-lung-cancer
#5
Wenyu Hu, Haiyan Wei, Keming Li, Pei Li, Jiamao Lin, Rui Feng
OBJECTIVES: Ubiquitin specific protease 32 (USP32) is a highly conserved but uncharacterized gene, which has been reported to be associated with growth of breast cancer cells. However, the role of USP32 in human small cell lung cancer (SCLC) has not been uncovered. The aim of this study was to investigate and evaluate the clinical significance of USP32 in patients with SCLC. MATERIALS AND METHODS: Expression of USP32 was firstly investigated using public online data sets and then determined in SCLC tissues and cell lines using quantitative real-time PCR, Western blotting and immunohistochemical staining...
June 9, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28523736/hyper-activation-of-%C3%AE-catenin-signalling-induced-by-ikk%C3%AE%C2%B5-inhibition-thwarts-colorectal-cancer-cell-proliferation
#6
Jie Chen, Jun Zhao, Xuan Chen, Chengming Ding, Katie Lee, Zeming Jia, Yaoting Zhang, Yuzheng Zhou, Chaoying Wei, Jiantai He, Zanxian Xia, Jian Peng
OBJECTIVE: Aberrant activation of Wnt/β-catenin signalling contributes significantly to the development of human colorectal cancers and β-catenin is the key signalling molecule transducing canonical Wnt/β-catenin signalling. Therefore, β-catenin is a promising therapeutic target for cancer treatment. This study demonstrates that the oncogenic IKKε kinase phosphorylates β-catenin to restrain its hyper activation, therefore promoting colorectal cancer (CRC) cell proliferation. MATERIALS AND METHODS: IKKε and β-catenin expression levels in human colorectal cancer tissues and cell lines were analysed by immunohistochemical staining and Western blotting...
May 19, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28464433/linc00152-a-pivotal-oncogenic-long-non-coding-rna-in-human-cancers
#7
REVIEW
Yang Yu, Jian Yang, Quanpeng Li, Boming Xu, Yifan Lian, Lin Miao
In recent years, increasing evidence has shown the potential role of long non-coding RNAs (lncRNAs) in multiple cancers. Deregulation of lncRNAs was detected being closely associated with many kinds of tumours where they can act as a tumour suppressor or accelerator. LINC00152 was identified as an oncogene involved in many kinds of cancers, such as gastric cancer, hepatocellular carcinoma, colon cancer, gallbladder cancer and renal cell carcinoma. Moreover, inhibition of LINC00152 can suppress proliferation, migration and invasion of the cancer cells...
May 2, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28464358/mir-1297-regulates-neural-stem-cell-differentiation-and-viability-through-controlling-hes1-expression
#8
Jiaolin Zheng, Dan Yi, Xiaodong Shi, Huaizhang Shi
OBJECTIVES: Neural stem cells (NSCs) are self-renewing, undifferentiated and multipotent precursors that can generate neuronal and glial lineages. MicroRNAs (miRNAs) are small non-coding RNAs that act crucial roles in cell proliferation, differentiation and migration. However, the role of miR-1297 in the development of NSCs is still unknown. MATERIALS AND METHODS: Primary NSCs were isolated from rat's embryos. The expression of miR-1297 and Hes1 were measured by qRT-PCR...
May 2, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28714177/curved-microstructures-promote-osteogenesis-of-mesenchymal-stem-cells-via-the-rhoa-rock-pathway
#9
Qi Zhang, Shiyu Lin, Tao Zhang, Taoran Tian, Quanquan Ma, Xueping Xie, Changyue Xue, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: Cells in the osteon reside in a curved space, accordingly, the curvature of the microenvironment is an important geometric feature in bone formation. However, it is not clear how curved microstructures affect cellular behaviour in bone tissue. MATERIALS AND METHODS: Rat primary bone marrow mesenchymal stem cells (BMSCs) on wavy microgrooves were exposed to PDMS substrates with various curvatures to investigate alterations in cellular morphology and osteogenic differentiation...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28714176/osteogenic-differentiation-of-3d-cultured-mesenchymal-stem-cells-induced-by-bioactive-peptides
#10
Vera Lukasova, Matej Buzgo, Vera Sovkova, Jana Dankova, Michala Rampichova, Evzen Amler
OBJECTIVES: Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III, bone morphogenetic protein 7 (BMP-7) and BMP-2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells (pMSCs). MATERIALS AND METHODS: pMSCs were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#11
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28319306/microrna-802-plays-a-tumour-suppressive-role-in-tongue-squamous-cell-carcinoma-through-directly-targeting-map2k4
#12
Xiaozhen Wu, Zuode Gong, Lanying Sun, Long Ma, Qibao Wang
OBJECTIVES: Tongue squamous cell carcinoma (TSCC) is the most common oral tumours. MicroRNAs play crucial roles in many cell processes including cell viability, development, apoptosis, migration and invasion. The role of miR-802 in the TSCC is still unknown. MATERIALS AND METHODS: The miR-802 expression in TSCC tissues and cell lines was determined by quantitative real-time polymerase chain reaction. CCK-8 assay was performed to measure the cell viability, while the cell invasion assay was used to determine the cell invasion...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28247446/the-clinical-significance-and-biological-function-of-large-tumour-suppressor-2-in-hepatocellular-carcinoma
#13
Rong Liang, Yan Lin, Chun-Ling Yuan, Zhi-Hui Liu, Yong-Qiang Li, Xiao-Ling Luo, Jia-Zhou Ye, Hai-Hong Ye
OBJECTIVES: Present evidence has suggested that large tumour suppressor 2 (LATS2) is abnormally expressed in most human cancer. However, the clinical and prognostic value in hepatocellular carcinoma (HCC) is still unknown. MATERIALS AND METHODS: Large tumour suppressor 2 mRNA and protein expression levels in HCC tissues and cell lines were detected by qRT-PCR, immunohistochemistry or Western blot. The correlation between LATS2 expression and clinicopathological factors was analysed through immunohistochemistry...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28244168/ccat2-a-novel-oncogenic-long-non-coding-rna-in-human-cancers
#14
REVIEW
Yu Xin, Zheng Li, Heyi Zheng, Matthew T V Chan, William Ka Kei Wu
Colon cancer-associated transcript 2 (CCAT2) was originally identified as an oncogenic long non-coding RNA in colorectal cancer. Since its discovery, the oncogenic role of CCAT2 has been increasingly demonstrated in human cancers. In this connection, CCAT2 upregulation is frequently reported and very often associated with tumour progression and poor clinical outcomes. Functionally, knockdown of CCAT2 could induce cancer cell apoptosis and suppress cell proliferation and invasiveness, suggesting that CCAT2 might be a therapeutic target...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28217977/mir-506-enhances-the-sensitivity-of-human-colorectal-cancer-cells-to-oxaliplatin-by-suppressing-mdr1-p-gp-expression
#15
Hui Zhou, Changwei Lin, Yi Zhang, Xiuzhong Zhang, Chong Zhang, Pengbo Zhang, Xingwang Xie, Zeqiang Ren
OBJECTIVES: Chemoresistance development represents a major obstacle to the successful treatment of colorectal cancer (CRC). The aim of this study was to elucidate the mechanism by which miR-506 reverses oxaliplatin chemoresistance in CRC. METHODS: In this study, miR-506 levels were measured in 74 patients with colon cancer via quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). We subsequently analysed the relationship between miR-506 expression and CRC patient survival via the Kaplan-Meier method...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28217951/cytotoxic-effect-of-zinc-oxide-nanoparticles-on-murine-photoreceptor-cells-via-potassium-channel-block-and-na-k-atpase-inhibition
#16
Chao Chen, Wenjuan Bu, Hongyan Ding, Qin Li, Dabo Wang, Hongsheng Bi, Dadong Guo
OBJECTIVE: Zinc oxide (ZnO) nanoparticles can exhibit toxicity towards organisms and oxidative stress is often hypothesized to be one of the most important factors. Nevertheless, the detailed mechanism of toxicity-induced by ZnO nanoparticles has not been completely addressed. The present study aimed to investigate the toxic effects of ZnO nanoparticles on the expression and activity of Na(+) /K(+) -ATPase and on potassium channel block. MATERIALS AND METHODS: In the present study, we explored the cytotoxic effect of ZnO nanoparticles on murine photoreceptor cells using lactate dehydrogenase (LDH) release assay, reactive oxygen species (ROS) determination, mitochondrial membrane potential (Δφm) measurement, delayed rectifier potassium current recordings and Na(+) /K(+) -ATPase expression and activity monitoring...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28205330/effect-of-matrix-stiffness-on-osteoblast-functionalization
#17
Tao Zhang, Shiyu Lin, Xiaoru Shao, Qi Zhang, Changyue Xue, Shu Zhang, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: Stiffness of bone tissue differs response to its physiological or pathological status, such as osteoporosis or osteosclerosis. Consequently, the function of cells residing in bone tissue including osteoblasts (OBs), osteoclasts and osteocytes will be affected. However, to the best of our knowledge, the detailed mechanism of how extracellular matrix stiffness affects OB function remains unclear. MATERIALS AND METHODS: We conducted a study that exposed rat primary OBs to polydimethylsiloxane substrates with varied stiffness to investigate the alterations of cell morphology, osteoblastic differentiation and its potential mechanism in mechanotransduction...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28205268/the-effect-of-delta-like-1-homologue-on-the-proliferation-and-odontoblastic-differentiation-in-human-dental-pulp-stem-cells
#18
Shengcai Qi, Yanhong Yan, Yue Wen, Jialiang Li, Jing Wang, Fubo Chen, Xiaoshan Tang, Guangwei Shang, Yuanzhi Xu, Raorao Wang
INTRODUCTION: This study aimed to investigate the functions of delta-like homologue 1 (DLK1) in the proliferation and differentiation of human dental pulp stem cells (hDPSCs). METHODS: Immunohistochemical analysis was used to determine the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), DLK1, NOTCH1 and p-ERK1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs. The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay respectively...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28145066/analysis-of-gene-expression-profiles-between-apical-papilla-tissues-stem-cells-from-apical-papilla-and-cell-sheet-to-identify-the-key-modulators-in-mscs-niche
#19
Shu Diao, Xiao Lin, Liping Wang, Rui Dong, Juan Du, Dongmei Yang, Zhipeng Fan
OBJECTIVES: The microenvironmental niche plays the key role for maintaining the cell functions. The stem cells from apical papilla (SCAPs) are important for tooth development and regeneration. However, there is limited knowledge about the key factors in niche for maintaining the function of SCAPs. In this study, we analyse the gene expression profiles between apical papilla tissues, SCAPs and SCAPs cell sheet to identify the key genes in SCAPs niche. MATERIALS AND METHODS: Microarray assays and bioinformatic analysis were performed to screen the differential genes between apical papilla tissues and SCAPs, and SCAPs and SCAPs cell sheet...
June 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28133828/a-defined-mix-of-cytokines-mimics-conditioned-medium-from-cultures-of-bone-marrow-derived-mesenchymal-stem-cells-and-elicits-bone-regeneration
#20
Wataru Katagiri, Kohei Sakaguchi, Takamasa Kawai, Yukiko Wakayama, Masashi Osugi, Hideharu Hibi
OBJECTIVES: We previously reported that conditioned medium from cultures of bone marrow-derived mesenchymal stem cells have strong potential to accelerate bone regeneration. We now examine in vitro and in vivo a defined cytokine cocktail that mimics the effects of conditioned medium on bone regeneration. MATERIALS AND METHODS: A cocktail of recombinant human insulin-like growth factor-1, vascular endothelial growth factor-A and transforming growth factor-β1 was prepared at concentrations similar to those in conditioned medium...
June 2017: Cell Proliferation
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