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Acta Cirúrgica Brasileira

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https://www.readbyqxmd.com/read/30328919/new-trends-in-translational-microsurgery
#1
Eiji Kobayashi
Technological advances such as optical instruments and surgical tools have enabled the considerable contributions of microsurgery to surgical therapies. Accordingly, surgical therapeutics has provided the latest information across a wide range of medical specialties, including immunology and pharmacology, despite specialization according to organs and organ systems. The International Society for Experimental Microsurgery, an academic organization, has utilized experimental microsurgery technology in the identification of curative concepts for diseases that remain difficult to treat...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328918/a-technical-note-on-low-cost-rat-laparoscopy-an-initial-experience
#2
José Marcus Raso Eulálio, Manoel Luiz Ferreira, Paulo César Silva, José Eduardo Ferreira Manso, Andrei Ferreira Costa Nicolau, Thales Penna de Carvalho, Julia Radicetti de Siqueira Paiva E Silva, Adrielle Rodas Fernandes, Alberto Schanaider
PURPOSE: To evaluate a novel and adapted low-cost set model for laparoscopic surgery in rats. METHODS: Nine Wistar rats underwent two different laparoscopic procedures, splenectomy (n=3) and distal pancreatectomy with splenectomy (n = 6), after assembling a low-cost set replacing the conventional one (monitor, micro camera, image processor, light source, laparoscope and insufflator). The new set included an Android Tablet 10.5 ", a 5mm USB Endoscope and semiautomatic sphygmomanometer monitor...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328917/experiences-with-basic-microsurgical-training-programs-and-skill-assessment-methods-at-the-university-of-debrecen-hungary
#3
Norbert Nemeth, Iren Miko, Istvan Furka
During the past decades microsurgical training programs together with skill assessment methods had been developing intensively worldwide. Concerning the teaching of basic techniques at various levels, we aimed to summarize the education program types and experiences at our department, in order to define the way of continuity. All in the hope that this summary might contain useful information for other educators as well. About 50 years ago, in the late 1960s, microsurgical basic education had been established in Debrecen...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328916/preservation-of-the-vagus-nerves-in-subtotal-esophagectomy-without-thoracotomy
#4
Eduardo Crema, Júverson Alves Terra Júnior, Marisa Carvalho Borges, Carlos Alfredo Salci Queiroz, Luciana Arantes Soares, Alex Augusto da Silva
PURPOSE: To evaluate the outcome of transhiatal esophagectomy without thoracotomy and with preservation of the vagal trunks for the treatment of advanced megaesophagus. METHODS: Between March 2006 and September 2017, it was performed 136 transhiatal esophagectomies without thoracotomy by laparoscopy, with preservation of the vagus nerves. All patients were evaluated pre and postoperatively for respiratory and nutritional aspects Post operatively, some surgical aspects were evaluated like radiology and endoscopy of the digestive tract...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328915/hyperbaric-oxygenation-on-adhesions-prevention-after-laparotomy-in-rats
#5
Simone Vargas Bento, Tarcizo Afonso Nunes, Ivana Durval Araújo, Roberto Carlos Oliveira E Silva, Paula Vieira Teixeira Vidigal, Ricardo Machado Carvalhais
PURPOSE: To evaluate the effects of hyperbaric oxygenation on prevention of adhesions in the abdominal cavity after laparotomy. METHODS: Fifty four rats underwent laparotomy; stitches were made in the four quadrant parietal peritoneum and abdominal cavity closure. Animals were divided into three groups: 1 - control; 2 - subjected to high pressures and oxygenation; 3 - subjected to 100% hyperbaric oxygenation. The animals in groups 2 and 3 were daily submitted to oxygenation hyperbaric chamber after surgery...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328914/potencial-of-different-hydroxyapatites-as-biomaterials-in-the-bone-remodeling
#6
Pedro Carvalho Cassino, Larissa Schimidt Rosseti, Osmar Ignácio Ayala, Marco Antônio Utrera Martines, Luciane Candeloro Portugual, Claudio Goncalves de Oliveira, Iandara Schettert Silva, Ruy de Araujo Caldas
PURPOSE: To analyze the therapeutic potentials of different hydroxyapatites used for the correction of bone defects in rats. METHODS: Forty rats, male, albino wistar, were distributed in 4 groups. They were submitted to a 3.5 mm defect in tibia. They received low purity hydroxyapatite, Strontium hydroxyapatite and hydroxyapatite doped with gallium, having a seven day evaluation time. Histopathology slides were stained with hematoxylin-eosin, for morphological evaluation...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328913/oxidative-stress-effects-in-the-uterus-placenta-and-fetus-of-pregnant-rats-submitted-to-acute-and-chronic-stress
#7
Adriano Silva Silveira, Ricardo Dutra Aydos, Rondon Tosta Ramalho, Iandara Schettert Silva, Ruy de Araujo Caldas, Abílio Torres Dos Santos Neto, Camila Tozaki Rodrigues
PURPOSE: To evaluate the effects of oxidative stress in pregnant rats submitted to acute and chronic stress, relating to alterations in the uterus, placenta and fetus. METHODS: Twenty-four female Wistar albino (Rattus norvegicus), were divided into four groups, for induction of oxidative stress the animals were submitted to cold and physical immobilization. Plasma fasting glucose and MDA were determined in all groups and the fetuses and placentas were measured. RESULTS: There were no statistical differences in the levels of malonic dialdehyde (MDA), however the averages of chronic stress group were higher compared to control groups, which could explain the observed adverse effects; there was no correlation between puppies' size, the weight of the placenta and MDA values...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328912/wound-healing-of-diabetic-rats-treated-with-moringa-oleifera-extract
#8
Ítalo Medeiros Azevedo, Irami Araújo-Filho, Marianny Maiara Antas Teixeira, Marília Daniela Ferreira de Carvalho Moreira, Aldo Cunha Medeiros
PURPOSE: To evaluate if Moringa oleifera leaf aqueous extract (ME) influences the healing of skin wounds of diabetic rats. METHODS: Wistar rats were used (6 rats/group). Group 1 received normal saline (NS) v.o. Group 2 received moringa extract (100mg/kg v.o) for 3 weeks. Groups 3 and 4: Streptozotocin (STZ) induced diabetes. Group 3 received NS; Group 4 received aqueous ME (100mg/kg) v.o.The wounds of groups 1 and 3 rats were topically treated with NS; wounds of groups 2 and 4 treated with 200µL of 10% ME...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328911/comparative-study-of-fibrosis-induced-by-marlex%C3%A2-parietex-composite%C3%A2-vicryl%C3%A2-and-ultrapro%C3%A2-meshes1
#9
COMPARATIVE STUDY
Maria de Lourdes Pessole Biondo-Simões, Arthur Andrade Sichciopi, Sérgio Ossamu Ioshii, Rogério Ribeiro Robes, Rachel Biondo-Simões
PURPOSE: To evaluate the fibrosis induced by four different meshes: Marlex®, Parietex Composite®, Vicryl® and Ultrapro®. METHODS: Histological cutouts of abdominal wall were analyzed with polarized light 28 days after the meshes implants and colorized by picrosirius to identify the intensity of collagen types I and III, and their maturation index. RESULTS: When the four groups were compared, the total collagen area analyzed was bigger in groups A and D, with no difference between them...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328910/intravascular-and-intraparenchymatous-hepatic-segmentary-sclerosis
#10
Guilherme Velloso Diniz, Andy Petroianu
PURPOSE: To evaluate the morphological effects of injected sclerosing agents into the liver. METHODS: This study was performed on twenty dogs, distributed into five groups: Group 1 (n = 5) - control, Group 2 (n = 5) - injection of 50% glucose solution inside hepatic parenchyma and animals followed during seven days, Group 3 (n = 10) - injection of ethanol inside hepatic parenchyma and animals distribution into two subgroups Subgroup 3A (n = 5) - followed during 24 hours and subgroup 3B (n = 5) - followed during seven days (group 3B), Group 4 (n = 5) - ethanol injection inside left portal vein branch and followed during 24 hours...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328909/rivastigmine-prevents-injury-induced-by-ischemia-and-reperfusion-in-rat-liver
#11
Agnaldo Bruno Chies, Paula Carolina Grande Nakazato, Maria Angélica Spadella, Patrícia Zorzi, Maria Cecília Jordani Gomes, Luiz Augusto Carneiro D'Albuquerque, Orlando Castro-E-Silva
PURPOSE: To evaluate whether pre-treatment with rivastigmine is able to attenuate the I/R induced lesions in rat liver. METHODS: SHAM animals or those submitted to I/R, non-treated or pre-treated with rivastigminine (2mg/kg) either 50 or 15 minutes before ischemia, were used. After I/R protocol, these animals were killed and their livers were harvested to measurement of the mitochondrial swelling as well as the malondialdehyde (MDA), nitrite and nitrate tissue concentration...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328908/tissue-microarray-technology-and-collagen-evaluation-to-analyze-surgical-trauma-performed-with-usual-blade-or-ultrasonic-harmonic-scalpels-in-rats
#12
COMPARATIVE STUDY
Octavio Antonio Azevedo da Costa-Filho, Mario Augusto Cray da Costa, Ana Maria Waaga-Gasser, Luiz Fernando Kubrusly, Luciane Bugmann Moreira de Oliveira, Luiz Martins Collaço, Maria Angélica Baron Magalhães, Martin Gasser, Osvaldo Malafaia, Jurandir Marcondes Ribas-Filho
PURPOSE: To compare wound healing performed with cold blade (CSB) and ultrasonic harmonic scalpel (UHS) in the abdominal aponeurosis of rats. METHODS: Eighty Wistar rats divided into two groups and underwent midline incision in the linea alba with cold blade and harmonic ultrasonic scalpel. Analysis were performed in subgroups of 10 animals after 3, 7, 14 and 21 days. Macroscopically was observed the presence of hematoma, infection, wound dehiscence, fistula and adherences...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328907/oxidative-stress-on-ischemia-reperfusion-injury-in-mice-with-non-alcoholic-hepatic-steatosis-or-steatohepatitis
#13
COMPARATIVE STUDY
Natalie Chaves Cayuela, Gabrielly Pascoa Negreti, Roberto Rasslan, Marcia Kiyomi Koike, Edna Frasson de Souza Montero
PURPOSE: To evaluate the oxidative stress, resulting from ischemia and hepatic reperfusion, in mice with non-alcoholic hepatic steatosis and steatohepatitis. METHODS: C57BL/6 male mice were used. Part of them were ob/ob mice, and the other part was fed with standard or MCD diets - this last used to develop steatohepatitis. The animals - MCD-I/R, ob/ob-I/R and I/R groups - were submitted to 30 minutes of partial hepatic ischemia, followed by reperfusion for 24 hours...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328906/role-of-moxifloxacin-dexamethasone-in-cardiac-histomorphometric-findings-among-wistar-rats-from-infected-mothers
#14
Gustavo Ithamar Souto Maior, Guilherme Veras Mascena, Valéria Wanderley Pinto Brandão Marquis, Carlos Alberto Figueiredo Filho, Alexandre Rolim da Paz, Líbia Cristina Rocha Vilela Moura, Carlos Teixeira Brandt
PURPOSE: To investigate cardiac changes in young rats, whose mothers underwent autogenic fecal peritonitis, during organogenesis phase and to evaluate the role of intravenous administration of moxifloxacin and dexamethasone in preventing infection-related cardiac changes. METHODS: A prospective histomorphometric study was performed on 29 hearts of Wistar four-month old rats. Animals were divided into three groups: Negative Control Group (NCG) included 9 subjects from healthy mothers; Positive Control Group (PCG) included 10 subjects from mothers with fecal peritonitis (intra-abdominal injection of 10% autogenic fecal suspension in the gestational period) and did not receive any treatment; and Intervention Group (IG), with 10 animals whose infected mothers received moxifloxacin and dexamethasone treatment 24 hours after induction of fecal peritonitis...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30328905/influence-of-low-level-laser-irradiation-on-osteocalcin-protein-and-gene-expression-in-bone-tissue1
#15
Fernando Russo Costa do Bomfim, Valeria Regina Gonzalez Sella, Ronaldo Luís Thomasini, Helio Plapler
PURPOSE: To evaluate osteocalcin gene and protein expression in vitro and in an in vivo model of ostectomy. METHODS: Twenty Wistar rats were assigned into two groups A (n=10, laser) and B (n=10, control). Ostectomy was performed in the femur diaphysis; the twenty fragments removed, composed in vitro groups named as in vivo (A and B) and cultivated in CO2 atmosphere for thirteen days. Low-level laser irradiation was performed in groups A (in vivo and in vitro) by an GaAlAs device (λ=808 nm, dose of 2J/cm2, power of 200mW, power density of 0...
September 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30208134/liver-ischemia-and-reperfusion-injury-pathophysiology-and-new-horizons-in-preconditioning-and-therapy
#16
REVIEW
Paula Carolina Grande Nakazato, João Paulo Victorino, Clarice Fleury Fina, Karina Dal Sasso Mendes, Maria Cecília Jordani Gomes, Paulo Roberto Barbosa Evora, Luiz Augusto Carneiro D'Albuquerque, Orlando Castro-E-Silva
It is well known that during hepatic operative procedures, it is often critical that the irrigation is interrupted to avoid possible bleeding, blood transfusions, variable intensities, and their short and long-term consequences. It was believed in the past that the flow interruption should not exceed 20 minutes, which limited the use of this maneuver. However, it has been postulated that ischemia could be maintained for more than 60 minutes in healthy livers. The present paper review includes: 1) A brief introduction to justify the rationale of the review design; 2) Aspects of the pathophysiology of the three stages of the liver ischemia-reperfusion injury; 3) The innate and acquired immunity; 4) Oxidative stress; 5) Apoptosis and autophagy, Some essential biomarkers (Tumor Necrosis Factor-α, nitric oxide, metalloproteinases); and, finally; 6) Preventive ("cheating") strategies, non-pharmacological and pharmacological options to treat the liver IR injury...
August 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30208133/simulated-training-of-a-laparoscopic-vesicourethral-anastomosis
#17
Eudes Fontenele Moraes Pinheiro, Márcio Alencar Barreira, Luiz Gonzaga de Moura Junior, Charles Jean Gomes de Mesquita, Rômulo Augusto da Silveira
PURPOSE: To develop a model and curriculum for simulated training of an effective and well accepted laparoscopic vesicourethral anastomosis (VUA). METHODS: Experimental longitudinal study of quantitative character. The sample consisted of 12 general surgery residents and 6 urology residents (R3). The training consisted of making twelve VUAs on synthetic organs. The training was divided into four sessions and accompanied by an instructor who performed positive feedback...
August 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30208132/the-role-of-vitamin-c-in-the-gene-expression-of-oxidative-stress-markers-in-fibroblasts-from-burn-patients
#18
Jessica Bonucci, Alfredo Gragnani, Marcelo Moraes Trincado, Victor Vincentin, Silvana Aparecida Alves Correa, Lydia Masako Ferreira
PURPOSE: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. METHODS: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups...
August 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30208131/comparative-safety-assessments-of-the-biosimilar-apz001-and-erbitux-in-pre-clinical-animal-models
#19
COMPARATIVE STUDY
Xiaofei Wang, Jianmin Guo, Xinyu Deng, Yuankeng Huang, Caiguo Ye, Huiqing Liang, Junhua Rao, Wei Yang
PURPOSE: To evaluate the toxicity of Erbitux as well as its biosimilar APZ001 antibody (APZ001) in pre-clinical animal models including mice, rabbits and cynomolgus monkeys. METHODS: We performed analysis of normal behavior activity, autonomic and non-autonomic nervous functions, nervous-muscle functions, nervous excitability and sensorimotor functions on CD-1 mice. Subsequently, we studied that effects of APZ001 and Erbitux on respiratory system, cardiovascular system and kidney in Cynomolgus monkey models and performed local tolerance experiments on New Zealand rabbits...
August 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30208130/prospective-clinical-assessment-of-tibial-tuberosity-advancement-for-the-treatment-of-cranial-cruciate-ligament-rupture-in-dogs
#20
Luciano Pereira de Barros, Laura Raquel Rios Ribeiro, Letícia Cordeiro de Pina Camondá Pereira, Fernando Lucas Maschio Ferreira, Maria Eduarda Bastos Andrade Moutinho da Conceição, Luis Gustavo Gosuen Gonçalves Dias
PURPOSE: To evaluate clinically dogs that underwent tibial tuberosity advancement (TTA) six months previously. METHODS: Dogs of various breeds, gender, weight, and age that had CCL rupture and underwent TTA for treatment were included in this study. Parapatellar arthrotomy was performed in all patients to assess the joint for a ruptured ligament and meniscal injury before the TTA. The appropriate cage for the TTA was chosen with planning surgery. The surgical procedure was performed according to the literature, using a modified Maquet technique...
August 2018: Acta Cirúrgica Brasileira
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