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Acta Cirúrgica Brasileira

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https://www.readbyqxmd.com/read/28076509/animal-models-of-obesity-in-rodents-an-integrative-review
#1
Melina Ribeiro Fernandes, Nayara Vieira de Lima, Karoline Silva Rezende, Isabela Caroline Marques Santos, Iandara Schettert Silva, Rita de Cássia Avellaneda Guimarães
PURPOSE: To perform an integrative review of the main animal disease models in rodents used for obesity. METHODS: Research was conducted in the CAPES Portal database using the following keywords "obesity animal models, diet and rodents", published between the years 2010 to 2016. We found 108 articles, of which 19 were selected and analyzed in full for this study. RESULTS: Larger part of publications occurred in the last 6 years, the rats (n = 10) were used in the same proportion mice (n = 10)...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076508/swing-mesh-versus-modified-kugel-mesh-for-primary-inguinal-hernia-repair-a-prospective-randomized-clinical-trial
#2
Zhiyuan Zhou, Xingchen Hu, Beili Zhang, Yan Gu
PURPOSE: To evaluate the safety and efficacy of a new mesh in the pre-peritoneal repair of inguinal hernia. METHODS: We randomly divided 120 patients undergoing pre-peritoneal repair into 2 groups between March 2012 and December 2013. The patients were randomized to receive the Swing mesh (n=60; study group) or the Modified Kugel mesh (n=60; control group). The primary end point of this study was to compare postoperative groin pain of the two groups. Complications, recurrence and analgesic use were also recorded...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076507/effects-of-cococonut-water-and-simvastatin-in-the-treatment-of-sepsis-and-hemorrhagic-shock-in-rats
#3
Vanessa de Fátima Lima Paiva Medeiros, Ítalo Medeiros Azevedo, Marília Daniela Ferreira Carvalho, Eryvaldo Sócrates Tabosa Egito, Aldo Cunha Medeiros
PURPOSE: To evaluate the effects of modified coconut water as fluid of resuscitation combined with simvastatin in hemorrhagic shock and sepsis model in rats. METHODS: Four groups of Wistar rats with hemorrhagic shock and abdominal sepsis were studied (n=8/group). Rats were bled and maintained at a mean blood pressure 35mmHg for 60min. They were then resuscitated with: 1) saline 0.9%; 2) coconut water+3% NaCl; 3) coconut water+NaCl 3%+simvastatin microemulsion (10 mg/kg i...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076506/stem-cells-from-adipose-tissue-improve-the-time-of-wound-healing-in-rats
#4
Camila Melo Ohashi, Fabio Alves Morikawa Caldeira, Denilson José Silva Feitosa-Junior, André Lopes Valente, Paulo Roberto Witter Dutra, Moysés Dos Santos Miranda, Simone do Socorro Damasceno Santos, Marcus Vinicius Henriques Brito, Otávio Mitio Ohashi, Edson Yuzur Yasojima
PURPOSE: To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS: Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylin-eosin was performed...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076505/therapeutic-effect-of-low-molecular-weight-chitosan-containing-sepia-ink-on-ethanol-induced-gastric-ulcer-in-rats
#5
Wei Zhang, Kang Liu, Lei Li, Yingxin Li, Xianxian Sui, Yinzhu Rao, Jiahao Wu, Qiuping Wu
PURPOSE: To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats. METHODS: Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections. RESULTS: Gastric empty was no significant differences among the groups (P > 0...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076504/the-role-of-bradykinin-in-lung-ischemia-reperfusion-injury-in-a-rat-lung-transplantation-model
#6
Zheng Tang, Zhiwei Wang, Zhipeng Hu, Min Zhang, Luocheng Li, Bowen Li
PURPOSE: To investigate the role of bradykinin in a rat lung transplantation (LTx) model and preliminarily discuss the relationship between bradykinin and CD26/DPP-4. METHODS: Rats were randomly divided into four groups: Control (CON), Sham, low potassium dextranglucose (LPD), and AB192 (n=15/group). Orthotopic single LTx was performed in the LPD and AB192 groups. The donor lungs were flush-perfused and preserved with low potassium dextranglucose (LPD) or LPD+CD26/DPP-4 catalytic inhibitor (AB192)...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076503/effect-of-s%C3%A4-lymar%C3%A4-n-on-ox%C3%A4-dat%C3%A4-ve-stress-and-l%C3%A4-ver-h%C3%A4-stopathology-%C3%A4-n-exper%C3%A4-mental-obstruct%C3%A4-ve-jaund%C3%A4-ce-model
#7
Ali Kemal Onalan, Salih Tuncal, Sibel Kilicoglu, Salih Celepli, Esra Durak, Bulent Kilicoglu, Erdinc Devrim, Aziz Mutlu Barlas, Kemal Kismet
PURPOSE: To investigate the effect of silymarin on oxidative stress and hepatic injury induced by obstructive jaundice in an experimental model. METHODS: Thirty Wistar-Albino type female rats were divided into 3 groups each including 10 rats. Only laparotomy was performed in group 1. Bile duct ligation was performed in group 2. In group 3, bile duct ligation was performed and orogastic silymarin 300 mg/kg/day dose was given for seven days. At the end of seven days, rats were sacrificed...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076502/carcinogenesis-in-rats-subjected-to-a-new-model-ureterosigmoidostomy-and-treated-with-l-lysine
#8
Alessandra Marques Dos Santos, Joao Paulo Ferreira Coelho, Camila de Carvalho Juanes, Rafael Barbosa de Azevedo, Nayanna de Oliveira Ramos Melo, Francisco Vagnaldo Fechine Jamacaru, Conceição Aparecida Dornelas
PURPOSE: To evaluate the effects of L-lysine on the intestinal and urothelial epithelium of rats subjected to ureterosigmoidostomy (new model for surgical carcinogenesis). METHODS: Forty-two rats, 9 weeks of age, were divided into 6 groups. Animals in groups A, B, C were subjected to ureterosigmoidostomy (US) and treated with L-lysine, celecoxib and H2O, respectively. Groups D, E and F (non-operated controls) received L-lysine, celecoxib and H2O, respectively. The L-lysine dose was 150 mg/kg and that of celecoxib was 20 mg/kg...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076501/evaluating-the-best-time-to-intervene-acute-liver-failure-in-rat-models-induced-by-d-galactosamine
#9
Lígia Patrícia de Carvalho Batista Éboli, Alcides Augusto Salzedas Netto, Ramiro Antero de Azevedo, Valéria Pereira Lanzoni, Tatiana Sugayama de Paula, Alberto Goldenberg, Adriano Miziara Gonzalez
PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) ...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28076500/histological-evaluation-of-capsules-formed-by-silicon-implants-coated-with-polyurethane-foam-and-with-a-textured-surface-in-rats
#10
Eduardo Nascimento Silva, Jurandir Marcondes Ribas-Filho, Nicolau Gregori Czeczko, Jan Pawel Andrade Pachnicki, Mário Rodrigues Montemor Netto, Leandro Cavalcante Lipinski, Lucia de Noronha, Joelmir Colman, João Otavio Zeni, Caroline Aragão de Carvalho
PURPOSE: To assess the capsules formed by silicone implants coated with polyurethane foam and with a textured surface. METHODS: Sixty-four Wistar albinus rats were divided into two groups of 32 each using polyurethane foam and textured surface. The capsules around the implants were analyzed for 30, 50, 70 and 90 days. Were analyzed the following parameters: foreign body reaction, granulation tissue, presence of myofibroblasts, neoangiogenesis, presence of synovial metaplasia, capsular thickness, total area and collagen percentage of type I and III, in capsules formed around silicone implants in both groups...
December 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982265/osteoarthritis-model-induced-by-intra-articular-monosodium-iodoacetate-in-rats-knee
#11
Sebastião Vieira de Morais, Nicolau Gregori Czeczko, Osvaldo Malafaia, Jurandir Marcondes Ribas, João Batista Santos Garcia, Marcelo Tizzot Miguel, Cassio Zini, Angeline Garcez Massignan
PURPOSE: To evaluate the usefulness of a knee osteoarthritis model through functional, radiological and microscopic changes of the synovial membrane. METHODS: Forty eight rats were divided randomly into two groups. The first received 0.9% saline in the joint and corresponded to the control group. The second was submitted to experimental osteoarthritis of the right knee induced by monosodium iodoacetate and corresponded to the osteoarthritis group. All animals were subjected to comparative tests of forced ambulation and joint movements, inability to articulate and tactile allodynia on day 1 post-experiment by forced ambulation (Roto-rod test), joint assessment of disability (weight bearing test) and assessment of tactile allodynia (Von Frey test)...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982264/influence-of-chlorpromazine-on-renal-histology-of-rats-submitted-to-ischemia-and-reperfusion-injury
#12
Júlio César Foiatto, Nicolau Gregori Czeczko, Eduardo Gabriel Guerber Wietzikoski, Osvaldo Malafaia, Thadeu Brenny, André Matos de Oliveira, Regina de Paula Xavier Gomes
PURPOSE: To analyze the influence of chlorpromazine on renal histology of rats submitted to ischemia and reperfusion injury. METHODS: Sixteen Wistar rats - split in two groups - have been used: control group, receiving 3 mg/kg isotonic saline solution through caudal vein, and, the chlorpromazine group, receiving 3 mg/kg-IV of such medication. The nephrectomy of the left kidney lower third was carried out; immediately, the test-drug was administrated. After 15 minutes of test-drug administration, the renal pedicle was clamped; in 60 minutes of ischemia it was released...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982263/glomerular-loss-after-arteriovenous-and-arterial-clamping-for-renal-warm-ischemia-in-a-swine-model
#13
Gustavo Ruschi Bechara, José Aurelino Damasceno-Ferreira, Leonardo Albuquerque Dos Santos Abreu, Waldemar Silva Costa, Francisco José Barcellos Sampaio, Marco Aurélio Pereira-Sampaio, Diogo Benchimol De Souza
PURPOSE: To evaluate the glomerular loss after arteriovenous or arterial warm ischemia in a swine model. METHODS: Twenty four pigs were divided into Group Sham (submitted to all surgical steps except the renal ischemia), Group AV (submitted to 30 minutes of warm ischemia by arteriovenous clamping of left kidney vessels), and Group A (submitted to 30 minutes of ischemia by arterial clamping). Right kidneys were used as controls. Weigh, volume, cortical volume, glomerular volumetric density (Vv[Glom]), volume-weighted glomerular volume (VWGV), and the total number of glomeruli were measured for each organ...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982262/characterization-of-an-experimental-model-of-progressive-renal-disease-in-rats
#14
Nilo César do Vale Baracho, Lucas Miranda Kangussu, Thiago Ruiz Rodrigues Prestes, Kátia Daniela da Silveira, Regina Maria Pereira, Natália Pessoa Rocha, Ana Cristina Simões E Silva
PURPOSE: To characterize an experimental model of progressive renal disease induced by different degrees of nephrectomy in rats. METHODS: Eighty male Wistar rats were divided into four experimental groups (n=20/group): sham surgery (control group), progressive degrees of nephrectomy leading to mild uremia (group 1), moderate uremia (group 2) and severe uremia (group 3). Ten animals of each group were followed for two or four weeks. At the end, blood and 24-hour urine samples were collected to determine renal function parameters...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982261/the-effects-of-enoxaparin-on-the-liver-in-experimental-pneumoperitoneum-model
#15
Turgut Cavusoglu, Kemal Kismet, Namik Ozcan, Berkay Kucuk, Aziz Mutlu Barlas, Bahadir Osman Bozkirli, Pinar Celepli, Mehmet Senes, Serkan Bolat, Sema Hucumenoglu
PURPOSE: To investigate the potential protective effects of enoxaparin against the adverse events of carbon dioxide (CO2) pneumoperitoneum. METHODS: Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO2 pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982260/effect-of-ozone-and-methylprednisolone-treatment-following-crush-type-sciatic-nerve-injury
#16
Omur Ozturk, Aysu Hayriye Tezcan, Yasemen Adali, Can Hakan Yıldırım, Ozgur Aksoy, Hatice Yagmurdur, Ali Bilge
PURPOSE: To assess and compare the histopathological effects of ozone therapy and/or methylprednisolone (MPS) treatment on regeneration after crush type sciatic nerve injury. METHODS: Forty Sprague-Dawley male rats were randomly allocated into four groups. Four groups received the following regimens intraperitoneally every day for 14 days after formation of crush type injury on sciatic nerve: Group I: ozone (20mcg/ml); Group II: methylprednisolone (2mg/kg); Group III: ozone (20 mcg/ml) and methylprednisolone (2mg/kg); Group IV: isotonic saline (0...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982259/hepatotoxicity-and-nephrotoxicity-of-3-bromopyruvate-in-mice
#17
Qiong Pan, Yiming Sun, Qili Jin, Qixiang Li, Qing Wang, Hao Liu, Surong Zhao
PURPOSE: To investigate the hepatotoxicity and nephrotoxicity of 3-Bromopyruvate (3BP) in mice. METHODS: Fifteen nude mice were grafted subcutaneously in the left flank with MDA-MB-231 cells, then all mice were divided into control group (PBS), 3BP group (8 mg/kg), positive group (DNR: 0.8 mg/kg) when tumor volume reached approximately 100 mm3. 28 days later, tumors, livers and kidneys were stored in 4 % formalin solution and stained with hematoxylin and eosin staining...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982258/the-positive-effect-of-botulinum-toxin-type-a-on-the-viability-of-random-flap-in-tobacco-exposed-in-rats
#18
Cristina Pires Camargo, Felipe Alexandre Fernandes, Michel Hyo Mi Lee, Luiza Campos Moreira Silva, Julio Morais Besteiro, Rolf Gemperli
PURPOSE: To evaluate the effect of Botulinum Toxin A in different time of tobacco exposure. METHODS: 60 male, Wistar rats were divided into two tobacco exposure groups: a 2- month or a 4-month regimen. After this period, these two groups were subdivided as two: saline solution(SS) or botulinum toxin A(Bonta), at the time of the surgery. Seven days before the SS or Bonta injection, the animals were submitted to a random flap (3x10cm). On the seventh postoperative day, all animals were assessed for total flap area, viable area, and the viable/ total area ratio...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982257/the-effects-of-passive-tobacco-smoking-on-the-microcirculation-of-the-abdominal-wall-in-rats
#19
Paulo Sandler, Bernardo Mastella, Diego Uchôa, Geraldo Pereira Jotz, Henrique Záchia Leão, Leandro Totti Cavazzola
PURPOSE: To analysis the effects of passive smoking on the microstructure of tissues of the abdominal wall regarding microcirculation, using histopathological study of the tobacco exposed rats. METHODS: Twenty four male Wistar rats were divided in Control Group (CG = 8 animals) and Exposition Groups (EG = 16 animals). EG was exposed to cigarette smoke 4x/day for 120 days, while CG was preserved from exposure. Food, water and housing were similar for both groups...
November 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27982256/effect-of-curcumin-on-visfatin-and-zinc-%C3%AE-2-glycoprotein-in-a-rat-model-of-non-alcoholic-fatty-liver-disease
#20
Changping Li, Juehong Li, Yun Chen, Xiaolin Zhong, Min Kang
PURPOSE: To investigate the effect of curcumin on visfatin and zinc-α2-glycoprotein (ZAG) expression levels in rats with non-alcoholic fatty liver disease (NAFLD). METHODS: Fifty-six male rats were randomly divided into a control group (n=16) and model group (n=40) and were fed on a normal diet or a high-fat diet, respectively. Equal volumes of sodium carboxymethyl cellulose (CMC) were intragastrically administered to the control group for 4 weeks. At the end of the 12th week, visfatin and ZAG protein expression levels were examined by immunohistochemistry...
November 2016: Acta Cirúrgica Brasileira
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