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Acta Cirúrgica Brasileira

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https://www.readbyqxmd.com/read/30020317/development-of-a-model-for-laparoscopic-cholecystectomy-video-assisted-training-a-randomized-study
#1
Francesco Mongelli, Davide La Regina, Michele Marengo, Ramon Pini, Andrea Saporito, Maurice FitzGerald, Matteo Di Giuseppe
PURPOSE: To identify the surgical trainee benefits through the use of video assisted training (VAT). METHODS: Twelve cases of uncomplicated laparoscopic cholecystectomy (LC) were selected. Edited video segments focused on essential intra-operative anatomical structure identification and critical surgical steps. Evaluation of these videos was constructed on a scoring system based on pre and post-teach tests. RESULTS: The mean value of the pre-teach score was 33...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020316/impact-of-1-25-oh-2-d-3-on-tg-content-in-liver-of-rats-with-type-2-diabetes
#2
Yan Yang, Beibei Liu, Ling Gao, Qi Li, He Wang, Liandi Wang
PURPOSE: To evaluate the effects of 1,25 dihydroxy vitamin D3 (1,25(OH)2D3) on the content of triglyceride (TG), as well as on the gene and protein expressions of adiponectin receptor 2 (AdipoR2), p38 mitogen-activated protein kinase (P38MAPK), and lipoprotein lipase (LPL) in the liver of rats with type 2 diabetes mellitus (T2DM) so as to provide theoretical basis for exploring the mechanism by which 1,25(OH)2D3 regulates TG. METHODS: Wistar rats were divided into four groups (n=25), with different treatments and detected the gene and protein expressions of AdipoR2, p38MAPK, and LPL in the liver tissue by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020315/a-curcumin-derivative-j147-ameliorates-diabetic-peripheral-neuropathy-in-streptozotocin-stz-induced-dpn-rat-models-through-negative-regulation-ampk-on-trpa1
#3
Juan Lv, Lanxiu Cao, Rui Zhang, Fu Bai, Pengfei Wei
PURPOSE: To investigate the specific molecular mechanisms and effects of curcumin derivative J147 on diabetic peripheral neuropathy (DPN). METHODS: We constructed streptozotocin (STZ)-induced DPN rat models to detected mechanical withdrawal threshold (MWT) in vivo using Von Frey filaments. In vitro, we measured cell viability and apoptosis, adenosine 5'-monophosphate-activated protein kinase (AMPK) and transient receptor potential A1 (TRPA1) expression using MTT, flow cytometry, qRT-PCR and western blot...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020314/cardioprotective-effect-of-lipstatin-derivative-orlistat-on-normotensive-rats-submitted-to-cardiac-ischemia-and-reperfusion
#4
Francisco Sandro Menezes-Rodrigues, Paolo Ruggero Errante, Regiane Miranda Ferreira, José Gustavo Padrão Tavares, Luciana de Paula, Erisvaldo Amarante Araújo, Tânia Carmem Peñaranda Govato, Eduardo Hiroshi Tikazawa, Maria do Carmo Maia Reis, Bráulio Luna-Filho, Renato Ribeiro Nogueira FerrazI, Itamar de Souza Oliveira-Júnior, Murched Omar Taha, Afonso Caricati-Neto
PURPOSE: To evaluate in vivo animal model of cardiac ischemia/reperfusion the cardioprotective activity of pancreatic lipase inhibitor of the orlistat. METHODS: Adult male Wistar rats were anesthetized, placed on mechanical ventilation and underwent surgery to induce cardiac I/R by obstructing left descending coronary artery followed by reperfusion to evaluation of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) with pancreatic lipase inhibitor orlistat (ORL)...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020313/synergistic-effect-of-honey-and-nigella-sativa-on-wound-healing-in-rats
#5
Seyed Mohammad Reza Javadi, Mamak Hashemi, Younes Mohammadi, Alireza MamMohammadi, Ali Sharifi, Hamid Reza Makarchian
PURPOSE: To investigate the therapeutic potential of honey, Nigella sativa (N. sativa) and their combination in rat model of excisional wound healing. METHODS: A circular excision wound was established in the back region of 50 Wistar rats. Subsequently, they were divided into 5 groups and daily topical administration of lanolin in the control group, honey in the honey group, cold-pressed N. sativa seed oil in the N. sativa groups, mix of 1:1 ratio of honey and N...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020312/an-experimental-study-on-the-preventive-effects-of-n-acetyl-cysteine-and-ozone-treatment-against-contrast-induced-nephropathy
#6
Omur Ozturk, Huseyin Avni Eroglu, Sefer Ustebay, Mehmet Kuzucu, Yasemen Adali
PURPOSE: To compare the preventive effects of N-acetyl cysteine (NAC), ozone preconditioning and ozone treatment against contrast-induced nephropathy (CIN) in an experimental rat model. METHODS: Thirty adult male Wistar rats were randomly distributed into five groups (n=6 for each group). Group I served as control and Group II had only contrast agent, while Group III received NAC and Group IV received intraperitoneal ozone 6 hours before and 6 hours after introduction of contrast agent...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020311/the-therapeutic-effects-of-curcumin-and-capsaicin-against-cyclophosphamide-side-effects-on-the-uterus-in-rats
#7
Ercan Yilmaz, Rauf Melekoglu, Osman Ciftci, Sevil Eraslan, Asli Cetin, Nese Basak
PURPOSE: To evaluate the impact of systemic cyclophosphamide treatment on the rat uterus and investigate the potential therapeutic effects of natural antioxidant preparations curcumin and capsaicin against cyclophosphamide side effects. METHODS: A 40 healthy adult female Wistar albino rats were used in this study. Rats were randomly divided into four groups to determine the effects of curcumin and capsaicin against Cyclophosphamide side effects on the uterus (n=10 in each group); Group 1 was the control group (sham-operated), Group 2 was the cyclophosphamide group, Group 3 was the cyclophosphamide + curcumin (100mg/kg) group, and Group 4 was the cyclophosphamide + capsaicin (0...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020310/interleukin-4-protects-from-chemotherapy-induced-peripheral-neuropathy-in-mice-modal-via-the-stimulation-of-il-4-stat6-signaling
#8
Qingbin Shi, Xiuying Cai, Guixiang Shi, Xingle Lv, Jinping Yu, Feng Wang
PURPOSE: To investigate the possible role of IL-4 signaling pathway in vincristine-induced peripheral neuropathy. METHODS: The mouse model of vincristine-induced peripheral neuropathy and interleukin (IL)-4 knockout mice were utilized to investigate the possible role of IL-4 signaling pathway in vincristine-induced peripheral neuropathy. Vincristine induced increased sensitivity to mechanical stimulation was measured by von Frey hair test 7 and 14 days after intraperitoneal administration of 0...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020309/effects-of-hypothermia-on-lung-inflammation-in-a-rat-model-of-meconium-aspiration-syndrome
#9
Ali Haydar Turhan, Aytuğ Atıcı, Serra Sürmeli
PURPOSE: To evaluate the effects of hypothermia treatment on meconium-induced inflammation. METHODS: Fifteen rats were instilled with human meconium (MEC, 1.5 mL/kg, 65 mg/mL) intratracheally and ventilated for 3 hours. Eight rats that were ventilated and not instilled with meconium served as a sham group. In MEC-hypothermia group, the body temperature was lowered to 33±0.5°C. Analysis of the blood gases, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in bronchoalveolar lavage (BAL) fluid samples, and histological analyses of the lungs were performed...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/30020308/antioxidant-extract-counteracts-the-effects-of-aging-on-cortical-spreading-depression-and-oxidative-stress-in-the-brain-cortex
#10
Mariana Séfora Bezerra Sousa, Iasmym Melissa Soares de Holanda, Heloísa Mirelle Costa Monteiro, Ângela Amâncio-Dos-Santos
PURPOSE: To investigate the effects of Murici extract on the brain excitability-dependent phenomenon known as cortical spreading depression (CSD) and on brain oxidative stress. METHODS: Adult and aged Wistar rats were supplemented with murici extract (150 mg/kg/day or 300 mg/kg/day) by gavage for fifteen days. Afterwards, the animals were submitted to a CSD electrophysiological recording and to brain oxidative stress evaluation. RESULTS: Our results showed that aging decreased CSD propagation velocity, catalase activity and glutathione/oxidized glutathione ratio (GSH/GSSG) in the brain cortex of the rats, and increased malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activity...
June 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924214/effect-of-red-propolis-on-hamster-cheek-pouch-angiogenesis-in-a-new-sponge-implant-model
#11
Nayanna de Oliveira Ramos Melo, Camila de Carvalho Juanes, Mayara Freire de Alencar Alves, Emiliano Tiago Melo Silva, Francisco Vagnaldo Fechine Jamacaru, Telma Leda Gomes de Lemos, Conceição Aparecida Dornelas
PURPOSE: To evaluate the effects of red propolis on cheek pouch angiogenesis in a hamster new model sponge implant. METHODS: Forty eight animals divided into eight groups. (Groups I-IV), the animals were treated for 15 days before and 10 days after sponge implantation. (Groups V-VIII), the animals were treated for 10 days after sponge implantation (GI and GV: red propolis 100 mg/kg, GII and GVI: celecoxib 20 mg/kg, GIII and GVII: 1% gum arabic 5 mL/kg, GIV and GVIII: distilled water 5 mL/kg)...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924213/evaluation-of-polyamide-surgical-mesh-as-an-abdominal-ventral-implant-in-rabbits
#12
Renata Gebara Sampaio Dória, Silvio Henrique de Freitas, Yumi de Barcelos Hayasaka, Maria Cristina Ferrarini Nunes Soares Hage, Ricardo de Francisco Strefezzi, Adriano Bonfin Carregaro, Gustavo Morandini Reginato, Carlos Eduardo Ambrósio, Alois Foltran Müller
PURPOSE: To evaluate abdominal ventral wound healing by using a specific biomaterial, a handmade polyamide surgical mesh. METHODS: A surgical incisional defect was made in ten rabbits to simulate a hernia in the ventral abdominal musculature. A polyamide surgical mesh was used in hernioplasty. They were monitored for surgical wound healing, and macroscopically and histologically evaluated at the end of the experiment. The polyamide surgical mesh did not cause foreign body reaction, pain, edema, or infection in the surgical site...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924212/influence-of-oophorectomy-on-glycemia-and-lipidogram
#13
Renan Kleber Costa Teixeira, Daniel Haber Feijó, André Lopes Valente, Luan Teles Ferreira de Carvalho, Hícaro Donato Granhen, Andy Petroianu, Nara Macedo Botelho
PURPOSE: To verify the influence of oophorectomy on lipidogram and glycemia. METHODS: Fifty six female rats were divided into the following 7 groups (n = 8): group 1 - sham group, group 2 - oophorectomy 30 days, group 3 - oophorectomy 35 days, group 4 - oophorectomy 40 days, group 5 - oophorectomy 45 days, group 6 - oophorectomy 70 days, group 7 - oophorectomy 55 days. Animals were following by number of days according the group. Was evaluated the serum levels of glucose and lipid profile...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924211/does-ureteral-access-sheat-usage-lead-to-permanent-damage-in-the-ureter-a-placebo-controlled-trial-in-a-rabbit-model
#14
Adnan Gücük, Gizem Söyler, Uğur Üyetürk, Burak Yılmaz, İsmail Nalbant, Sebahat Gücük, Aysel Kükner, Ayhan Çetinkaya
PURPOSE: To evaluate the clinical stenosis or precursor histological changes that ureteral access sheaths commonly used in ureteroscopic surgeries may cause in the long term in ureter. METHODS: In this study, the animals were divided into 9 groups and according to their groups, ureters of the rabbits were endoscopically fitted with 2F and 3F ureter catheters. The catheters were left in place and withdrawn after a specified period of time. All the ureters were excised and evaluated macroscopically, microscopically and histologically...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924210/effects-of-remote-ischemic-preconditioning-and-topical-hypothermia-in-renal-ischemia-reperfusion-injury-in-rats
#15
Guilherme Lang Motta, Pablo Cambeses Souza, Emanuel Burck Dos Santos, Silvia Regina Bona, Pedro Guilherme Schaefer, Caetano Araújo Torres Lima, Norma Anair Possa Marroni, Carlos Otávio Corso
PURPOSE: To evaluate whether combining hypothermia and remote ischemic preconditioning (RIPC) results in protection from ischemia-reperfusion (IR). METHODS: Thirty-two Wistar rats underwent right nephrectomy and were randomly assigned to four experimental protocols on the left kidney: warm ischemia (group 1), cold ischemia (group 2), RIPC followed by warm ischemia (group 3), and RIPC followed by cold ischemia (group 4). After 240 minutes of reperfusion, histological changes in the left kidney, as well as lipid peroxidation and antioxidant enzyme activity, were analyzed...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924209/capsiate-treatment-in-liver-surgeries-may-compromise-its-recovery
#16
Pedro Paulo Barros, Gisele Mara Silva Gonçalves, Gustavo Henrique da Silva, Maria Clara Villaça Dias Bastos, Loren Nogaroto Ramos, Marilia Marinello Fernandes
PURPOSE: To investigate the effects of capsiate treatment on hepatic hyperplasia in partially hepatectomized rats. METHODS: The animals were divided into a Capsiate group (CPH), a Capsiate Post-Partial Hepatectomy group (CPPH) and a Partial Hepatectomy Control group (PH). CPH and CPPH animals received 60 mg/kg/day Capsiate for 30 days. Next, the rats underwent partial hepatectomy. CPPH animals continued to receive treatment for 48 h after partial hepatectomy. Liver tissue and intracardiac blood samples were obtained 24 or 48 h after PH...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924208/fecal-peritonitis-in-aging-rat-model-therapeutic-response-to-different-antibiotic-strategies
#17
Guilherme Veras Mascena, Carlos Alberto Figueiredo Filho, Marcos Antônio Xavier de Lima Júnior, Thárcia Kiara Beserra Oliveira, Diego Nery Benevides Gadelha, Maria Cecília Santos Cavalcanti Melo, Carlos Teixeira Brandt
PURPOSE: To evaluate the response of aging rats with sepsis to two different antibiotic regimens. METHODS: The study was conducted with 30 aging rats (18 month-old) with autologous feces peritonitis. The animals were divided into three groups: Group 0 received no therapeutic intervention (control), while Group 1 received a single dose of 40 mg/kg meropenem and Group 2 received a single dose of 20 mg/kg moxifloxacin. The intervention in both Groups was made 6 hours after induction of peritonitis...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924207/proliferation-of-human-adipose-tissue-derived-stem-cells-stimulated-by-oil-rich-in-thymol-of-lippia-origanoides
#18
Felipe Nunes Brito, Fabiel Spani Vendramin, Cinthia Távora de Albuquerque Lopes, Mayra Pauline Ribeiro Costa, Otávio Mitio Ohashi, José Guilherme Soares Maia, Lydia Masako Ferreira, Joyce Kelly do Rosário da Silva, Moysés Dos Santos Miranda
PURPOSE: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. METHODS: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29924206/effect-of-hyperbaric-oxygenation-on-the-expression-of-glutathione-peroxidase-4-and-lactoperoxidase-genes-in-the-lung-of-isogenic-mice-after-ischemia-reperfusion-injury-in-the-small-bowel
#19
Adauto Tsutomu Ikejiri, Frederico Somaio Neto, Paulo Roberto Bertoletto, José Carlos Chaves, Alexandre Key WakateTeruya, Cândida Aparecida Leite Kassuya, Murched Omar Taha, Djalma José Fagundes
PURPOSE: To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR). METHODS: Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to anesthesia, laparotomy, small bowel ischemia for 60 minutes and reperfusion for 60 minutes; and three groups treated with HBO during ischemia (HBOG + I), during reperfusion (HBOG + R) and during ischemia and reperfusion (HBOG + IR)...
May 2018: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29768541/vascular-endothelial-growth-factor-association-with-angiopoietin-1-promotes-improvement-in-ventricular-function-after-ischemic-cardiomyopathy-induced-in-mini-pigs
#20
André Lacerda de Abreu Oliveira, Jussara Peters Scheffer, Melissa Markoski, Andreia Koche, Alexsandra Balbinot, Fernanda Antunes, Renato Kalil
PURPOSE: To investigate the safety and clinical, hemodynamic and tissue improvement ability in mini pigs undergoing cell and gene therapy for the treatment of acute myocardial infarction. METHODS: Thirty-two mini pigs Br1 lineage, 12 months old, undergoing induction of acute myocardial infarction by occlusion of the diagonal branch of the paraconal coronary. They were divided into 4 groups: one control group and 3 treatment groups (cell therapy and gene cell therapy)...
April 2018: Acta Cirúrgica Brasileira
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