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Acta Cirúrgica Brasileira

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https://www.readbyqxmd.com/read/29319737/a-non-living-effective-model-for-microvascular-training
#1
Cristina Pires Camargo, Daniel Imbassahy de Sa Bittencourt de Camara E Silva, Fauze Camargo Maluf, Julio Morais-Besteiro, Rolf Gemperli
PURPOSE: To introduce a nonliving microvascular training model based on vessels diameter and feasibility. METHODS: We dissected ten oxen tongues, and divided the pedicles into three-thirds: proximal, middle and distal. We measured the external vessels diameter in all regions. We performed a descriptive statistical analysis. Three students (two beginner level and one intermediate level) performed this training. We evaluated the confidence, according Likert scale...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319736/new-virtual-tool-for-accurate-evaluation-of-facial-volume
#2
Rodrigo de Faria Valle Dornelles, Nivaldo Alonso
No abstract text is available yet for this article.
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319735/tibial-tunnel-enlargement-and-joint-instability-after-anterior-cruciate-ligament-reconstruction-a-prospective-comparison-between-autograft-and-allograft
#3
Qiang Zhang, Guangliang Hao, Mingyong Gu, Xuecheng Cao
PURPOSE: To investigate tibial tunnel widening and knee instability after ACL reconstruction with hamstring autograft or irradiated soft tissue allograft. METHODS: Eight-two patients were divided into two groups: autograft group and allograft group. Radiographic and clinical evaluations were performed. RESULTS: Seventy patients were followed up with median of 36.3 months (range 36-38 months). Tibial tunnel widening was at or greater than 30% for nine patients in the autograft group and 15 patients in the allograft group (P = 0...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319734/a-prototype-single-port-device-for-pressurized-intraperitoneal-aerosol-chemotherapy-technical-feasibility-and-local-drug-distribution
#4
Rafael Seitenfus, Paulo Roberto Walter Ferreira, Gabriel Oliveira Dos Santos, Rafael José Vargas Alves, Antonio Nocchi Kalil, Eduardo Dipp de Barros, Olivier Glehen, Thaís Andrade Costa Casagrande, Eduardo Aimoré Bonin, Edison Martins da Silva Junior
PURPOSE: To evaluate the technical feasibility and homogeneity of drug distribution of pressurized intraperitoneal aerosol chemotherapy (PIPAC) based on a novel process of intraperitoneal drug application (multidirectional aerosolization). METHODS: This was an in vivo experimental study in pigs. A single-port device was manufactured at the smallest diameter possible for multidirectional aerosolization of the chemotherapeutic drug under positive intraperitoneal pressure...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319733/biomechanic-and-histologic-analysis-of-fibroblastic-effects-of-tendon-to-bone-healing-by-transforming-growth-factor-%C3%AE-1-tgf-%C3%AE-1-in-rotator-cuff-tears
#5
Chong Zhang, Yu-Jie Liu
PURPOSE: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. METHODS: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319732/a-fatal-and-metabolic-experimental-hemorrhagic-shock-in-immature-swine
#6
Simone Alves Dos Santos Ferreira, Ana Cristina de Moraes, Renato Giuseppe Giovanni Terzi, Evandro Luis Assis Ferreira, William Adalberto Silva, Marcos Mello Moreira
PURPOSE: To use blood lactate (BL) as an end-point metabolic marker for the begin resuscitation of volume replacement in experimental hemorrhagic shock. METHODS: Group I (n=7) was not bled (Control). Animals in Group II (n=7) were bled to a MAP of 30mmHg in thirty minutes. Hemodynamic and metabolic data were recorded at Baseline, at 30, 60 and 120 minutes after Baseline. The animals were intubated in spontaneous breathing (FIO2=0.21) with halothane. RESULTS: Group I all survived...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319731/comparison-among-bone-marrow-mesenchymal-stem-and-mononuclear-cells-to-promote-functional-recovery-after-spinal-cord-injury-in-rabbits
#7
Antônio Filipe Braga Fonseca, Jussara Peters Scheffer, Arthur Giraldi-Guimarães, Bárbara Paula Coelho, Raphael Mansur Medina, André Lacerda Abreu Oliveira
PURPOSE: To investigate the efficacy of allogeneic mesenchymal stem-cells and autologous mononuclear cells to promote sensorimotor recovery and tissue rescue. METHODS: Female rabbits were submitted to the epidural balloon inflation method and the intravenous cells administrations were made after 8 hours or seven days after injury induction. Sensorimotor evaluation of the hindlimbs was performed, and the euthanasia was made thirty days after the treatment. Spinal cords were stained with hematoxylin and eosin...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319730/effect-of-different-commercial-fat-sources-on-brain-liver-and-blood-lipid-profiles-of-rats-in-growth-phase
#8
Michel Cardoso de Angelis-Pereira, Maria de Fátima Píccolo Barcelos, Juciane de Abreu Ribeiro Pereira, Rafaela Corrêa Pereira, Raimundo Vicente de Souza
PURPOSE: To investigate the fatty acid content of different fat sources and evaluate the effect of them on plasma and hepatic lipids and on the fatty acid profile of the brain tissue of Wistar rats. METHODS: Thirty male albino Wistar rats received for 59 days, the following diets: diet added of margarine with low content of polyunsaturated fatty acids (PUFA); diet added of margarine with high content of PUFA; diet added of butter; diet added of hydrogenated vegetable fat; diet added of soybean oil...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319729/effects-of-nivolumab-in-peritoneal-carcinamatosis-of-malign-melanoma-in-mouse-model
#9
Ozgul Duzgun, Inanc Samil Sarici, Serkan Gokcay, Kivilcim Eren Ates, Mehmet Bertan Yılmaz
PURPOSE: To evaluate the efficacy of nivolumab and comparison with dacarbazine (DTIC) on peritoneal carcinomatosis of malignant melanoma in mouse model. METHODS: Mouse skin melanoma cells was injected under the capsule of the peritoneal surface in the left side of the abdomen. On postoperative day ten, mouses randomised into three groups. Group 1: Control, Group 2: HIPEC (Hyperthermic intraperitoneal chemotherapy) with DTIC and Group 3: HIPEC with Nivolumab. After the sacrification on postoperative day fifteen, peritoneum evaluated macroscopically and histopathologically by using peritoneal regression grading score (PRGS)...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29319728/cholecystectomy-during-ceftriaxone-therapy-a-translational-study-with-a-new-rabbit-model
#10
Marcel Vasconcellos, Keren Cozer, Victor Senna Diniz, Wagner Baetas-da-Cruz, Manoel Luiz Ferreira, Paulo Cesar Silva, Alberto Schanaider
PURPOSE: To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment with intravenous ceftriaxone in a new rabbit model. METHODS: New Zealand rabbits were treated with intravenous ceftriaxone or saline for 21 days. Ultrasound monitoring of the gallbladder was performed every seven days until the 21st day when histopathology, immunohistochemistry for proliferating cell nuclear antigen (PCNA), pro-caspase-3 and CD68, liver enzyme biochemistry, and chromatography analysis of the bile and sediments were also performed...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236803/aldefluor-protocol-to-sort-keratinocytes-stem-cells-from-skin
#11
Samuel Marcos Ribeiro Noronha, Alfredo Gragnani, Thiago Antônio Calado Pereira, Silvana Aparecida Alves Correa, Jessica Bonucci, Lydia Masako Ferreira
PURPOSE: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. METHODS: Activated Aldefluor® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. RESULTS: Best results were obtained with Aldefluor® 1...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236802/tadalafil-protector-effect-during-ischemia-reperfusion-in-rats
#12
Eduardo Gabriel Gerber Wietzikoski, Julio Cesar Foiatto, Nicolau Gregori Czeczko, Osvaldo Malafaia, Fernando Cesar Koleski, Tiago Cesar Mierzwa, Regina de Paula Xavier Gomes
PURPOSE: To evaluate histological parameters in rat renal tissue after tadalafil use during hot ischemia for 45 minutes and reperfusion for 24 hours. METHODS: Twenty rats were divided into 2 groups. In the experimental group 10 mg/kg of tadalafil was used per gavage before the procedure. All cases underwent left partial nephrectomy, followed by 45 minutes of warm ischemia. Left nephrectomy of the remaining kidney was performed after 24 hours from the initial procedure...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236801/effect-of-atenolol-pre-treatment-in-heart-damage-in-a-model-of-intestinal-ischemia-reperfusion
#13
Mieko Okada, Luiz Fernando Reis Falcão, David Ferez, José Luiz Martins, Paolo Ruggero Errante, Francisco Sandro Menezes Rodrigues, Afonso Caricati-Neto, Márcia Marinho, Guilherme Fenelon, Itamar Souza
PURPOSE: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. METHODS: Adult Wistar male rats were randomly (n=8), anesthetized and divided in: Sham: submitted to operation only; group SS+IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+I+AT+R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236800/topical-action-of-buriti-oil-mauritia-flexuosa-l-in-myositis-induced-in-rats
#14
Marília Ursulino Barbosa, Marcello de Alencar Silva, Esmeralda Maria Lustosa Barros, Margarida Ursulino Barbosa, Rayssilane Cardoso de Sousa, Mateus Aguiar da Costa Lopes, Nayana Pinheiro Machado de Freitas Coelho
PURPOSE: To analyze the topical effects of Buriti oil (Mauritia flexuosa L.) in induced myositis in rats. METHODS: Thirty six male rats divided into three groups: Control group (C), induced myositis group (MI) and induced myositis group reated with Mauritia flexuosa L. (MT). After inducing myositis with 1% acetic acid, was topically applied 0.5 ml of Mauritia flexuosa L.extract on the posterior region of the right gastrocnemius muscle in animals belonging to group MT, for 7 and 14 days...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236799/perconditioning-associated-to-hypertonic-saline-solution-on-liver-function-improvement-after-ischemia-reperfusion-injury
#15
Rubens Fernando Gonçalves Ribeiro, Rodrigo Paracampo Couteiro, Andrew Moraes Monteiro, Ivone Aline da Silva Rodrigues, Lainy Carollyne da Costa Cavalcante, Eduardo Henrique Herbster Gouveia, Lucas Nascimento Galvão, Luiz Renato Oliveira Lopes, Edson Yuzur Yasojima, Marcus Vinicius Henriques Brito
PURPOSE: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. METHODS: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236798/the-expression-of-endothelial-and-inducible-nitric-oxide-synthase-and-apoptosis-in-intestinal-ischemia-and-reperfusion-injury-under-the-action-of-ischemic-preconditioning-and-pentoxifylline
#16
Teresinha Regina Ribeiro de Oliveira, Geraldo Ferreira de Oliveira, Ricardo Santos Simões, Suellen Maurim Feitosa, Eduardo Hiroshi Tikazawa, Hugo Pequeno Monteiro, Djalma José Fagundes, Murched Omar Taha
PURPOSE: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. METHODS: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes)...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236797/nandrolone-decanoate-appears-to-increase-bone-callus-formation-in-young-adult-rats-after-a-complete-femoral-fracture
#17
Ana Paula Franttini Garcia Moreno Guimarães, Mariana Maloste Butezloff, Ariane Zamarioli, João Paulo Mardegan Issa, José Batista Volpon
PURPOSE: To evaluate the influence of nandrolone decanoate on fracture healing and bone quality in normal rats. METHODS: Male rats were assigned to four groups (n=28/group): Control group consisting of animals without any intervention, Nandrolone decanoate (DN) group consisting of animals that received intramuscular injection of nandrolone decanoate, Fracture group consisting of animals with a fracture at the mid-diaphysis of the femur, and Fracture and nandrolone decanoate group consisting of animals with a femur fracture and treatment with nandrolone decanoate...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236796/hyperbaric-oxygenation-and-the-genic-expression-related-to-oxidative-stress-in-the-heart-of-mice-during-intestinal-ischemia-and-reperfusion
#18
Frederico Somaio, Adauto Tsutomo Ikejiri, Paulo Roberto Bertoletto, José Carlos Chaves, Roberto Teruya, Djalma José Fagundes
PURPOSE: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. METHODS: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBO-IRG, which received HBO during ischemia and reperfusion...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236795/comparative-study-of-intraperitoneal-adhesions-related-to-light-weight-polypropylene-mesh-and-type-i-polymerized-and-purified-bovine-collagen-coated-light-weight-polypropylene-mesh-in-rabbits
#19
Diego Paim Carvalho Garcia, Clarissa Santos, Cristiana Buzelin Nunes, Marcelo Araújo Buzelin, Andy Petroianu, Luiza Ohasi de Figueiredo, Andreia Souto da Motta, Cristiane de Barros Gaspar, Luiz Ronaldo Alberti
PURPOSE: To compare the effectiveness of light-weight polypropylene mesh coated with polymerized and purified bovine type I collagen (Surgidry HNB) in the treatment of abdominal wall defect and the degree of adhesion formation. METHODS: Two types of polypropylene mesh were implanted after creation of defect measuring 6.0cm X 5.5cm in the anterior abdominal wall of 32 male New Zealand breed rabbits, divided in two groups (n = 32): (1) light-weigh macroporous polypropylene, (2) type I polymerized and purified bovine collagen coated light-weigh macroporous polypropylene...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29236794/qualitative-analysis-of-the-viability-of-autogenous-fat-grafts-grafted-in-different-environments-of-interstitial-pressure-preliminary-results-and-description-of-a-new-experimental-model-in-mini-pigs
#20
Eduardo Gustavo Pires de Arruda, Alexandre Mendonça Munhoz, Walter Matsumoto, Thiago Ueda, Renata de Almeida Coudry, Rolf Gemperli
PURPOSE: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. METHODS: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP...
November 2017: Acta Cirúrgica Brasileira
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