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European Cytokine Network

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https://www.readbyqxmd.com/read/29187340/enzyme-linked-immunosorbent-spot-elispot-monitoring-of-cytokine-producing-cells-for-the-prediction-of-acute-rejection-in-renal-transplant-patients
#1
Fatemeh Mohammadi, Ghasem Solgi, Maryam Tajik, Pedram Ahmadpoor, Hasan Nikoeinejad, Behzad Einollahi, Banafsheh Nazari, Mahboob Lessan-Pezeshki, Aliakbar Amirzargar
The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for meanly 3 years post-transplantation. Blood samples were collected pre-transplant, 2, 4 and 12 weeks post-transplant. The frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined in all time-points using ELISPOT assay...
September 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/29187339/sophocarpine-suppress-inflammatory-response-in-human-fibroblast-like-synoviocytes%C3%A2-and-in-mice-with-collagen-induced-arthritis
#2
Lihua Zhu, Liyan Zhu
Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting nearly 1% of adults worldwide. This study aimed to investigate whether sophocarpine is a potential drug for treating RA. The cytotoxicity of sophocarpine to RA-fibroblast-like synoviocytes (FLSs) was evaluated using 3-[4,-dimethylthiazol-2-y]-2,5-diphenyl-tetrazolium bromide (MTT) assays kit and released lactate dehydrogenase (LDH) assays. The transcription of proinflammatory cytokines in RA-FLSs was analyzed by reverse transcription and real-time polymerase chain reaction (RT-PCR)...
September 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/29187338/effects-of-interleukin-17a-il-17a-neutralization-on-murine-hepatitis-virus-mhv-a59-infection
#3
José L Aparicio, Macarena Ottobre, Maite Duhalde Vega, Jean-Paul Coutelier, Jacques Van Snick, Lilia A Retegui
Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti-IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased...
September 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/29187337/prr-signaling-during-in-vitro-macrophage-differentiation-from-progenitors-modulates-their-subsequent-response-to-inflammatory-stimuli
#4
Alba Martínez, Cristina Bono, Javier Megías, Alberto Yáñez, Daniel Gozalbo, M Luisa Gil
Toll-like receptor (TLR) agonists drive hematopoietic stem and progenitor cells (HSPCs) to differentiate along the myeloid lineage in vitro and also in vivo following infection. In this study, we used an in vitro model of HSPC differentiation to investigate the functional consequences (cytokine production) that exposing HSPCs to various pathogen-associated molecular patterns (PAMPs) and Candida albicans cells have on the subsequently derived macrophages. Mouse HSPCs (Lin- cells) were cultured with GM-CSF to induce macrophage differentiation in the presence or absence of the following pattern recognition receptor (PRR) agonists: Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand), depleted zymosan (which only activates Dectin-1), or inactivated C...
September 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840839/buprenorphine-differentially-affects-m1-and-m2-polarized-macrophages-from-human-umbilical-cord-blood
#5
Juan Sun, Wei Guo, Xingguang Du
As a partial μ-opioid receptor agonist with long half-life time, buprenorphine has been widely used to relieve chronic cancer and nonmalignant pain. The maintenance of chronic pain involves inflammation; however whether buprenorphine has anti-inflammation property remains unclear. Macrophages, the immune cells that initiate and maintain inflammation, were isolated from human umbilical cord blood, and were polarized into M1 or M2 macrophages with IFN-γ in the presence of lipopolysaccharide (LPS) or IL-4, respectively...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840838/the-role-of-host-immune-cells-and-borrelia-burgdorferi-antigens-in-the-etiology-of-lyme-disease
#6
Dennis Verhaegh, Leo A B Joosten, Marije Oosting
Lyme disease is a zoonosis caused by infection with bacteria belonging to the Borrelia burgdorferi species after the bite of an infected tick. Even though an infection by this bacterium can be effectively treated with antibiotics, when the infection stays unnoticed B. burgdorferi can persist and chronic post-treatment Lyme disease syndrome is able to develop. Although a cellular and humoral response is observed after an infection with the Borrelia bacteria, these pathogens are still capable to stay alive. Several immune evasive mechanisms have been revealed and explained and much work has been put into the understanding of the contribution of the innate and adaptive immune response...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840837/characterization-of-a-long-isoform-of-il-1r8-tir8-sigirr
#7
Maria Giovanna Vilia, Marta Tocchetti, Eleonora Fonte, Ilenia Sana, Marta Muzio
IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840836/predictive-ability-of-circulating-osteoprotegerin-as-a-novel-biomarker-for-early-detection-of-acute-kidney-injury-induced-by-sepsis
#8
Mona Schaalan, Waleed Mohamed
Though significant progress has been made towards new diagnostic approaches for early detection of acute kidney injury (AKI) induced by different factors, there is still an urgent demand for a more specific and predictive biomarker for each type. The aim of this study is to unravel the potential diagnostic utility of circulating osteoprotegerin (OPG) in septic patients who developed AKI in the ICU, compared to cystatin C (a renal function maker) and KIM-1 (a kidney damage marker). Eighty patients (male = 43, female = 37) with ages ranging from 42 to 46 years and with sepsis, 40 of whom developed AKI, and 30 healthy controls were enrolled in this prospective study...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840835/skin-innate-immune-response-to-flaviviral-infection
#9
Magali Garcia, Michel Wehbe, Nicolas Lévêque, Charles Bodet
Skin is a complex organ and the largest interface of the human body exposed to numerous stress and pathogens. Skin is composed of different cell types that together perform essential functions such as pathogen sensing, barrier maintenance and immunity, at once providing the first line of defense against microbial infections and ensuring skin homeostasis. Being inoculated directly through the epidermis and the dermis during a vector blood meal, emerging Dengue, Zika and West Nile mosquito-borne viruses lead to the initiation of the innate immune response in resident skin cells and to the activation of dendritic cells, which migrate to the draining lymph node to elicit an adaptive response...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840844/increased-serum-il-17a-and-th2-cytokine-levels-in-patients-with-severe-uncontrolled-asthma
#10
Takehiro Hasegawa, Hitoshi Uga, Akio Mori, Hirokazu Kurata
Asthma is a syndrome of chronic bronchial inflammation and airway remodelling. Initially, asthma has been categorized into atopic and nonatopic types, based on antigen-specific IgE levels. Moreover, recently, asthma has been classified into different endotypes based on its pathophysiology, leading to the selection of the most optimal and effective therapies. Although T helper cell type 2 (Th2) cytokines were proven to play critical roles in atopic asthma, IL-17A has been reported to be involved in severe refractory asthma...
March 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840843/interferon-gamma-release-assay-in-response-to-pe35-ppe68-proteins-a-promising-diagnostic-method-for-diagnosis-of-latent-tuberculosis
#11
Shima Mahmoudi, Babak Pourakbari, Setareh Mamishi
Tuberculosis control relies on the identification and preventive treatment of people who are latently infected with Mycobacterium tuberculosis (Mtb). PE/PPE proteins have been reported to elicit CD4 and/or CD8 responses either in the form of whole recombinant proteins or as individual peptides. Very few of the PE and PPE proteins have been previously tested for responses in patients with TB and healthy donors. This is the first study to evaluate the Interferon Gamma Release Assay (IGRA) after stimulation with PE35 and PPE68...
March 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840842/chemokine-and-chemokine-receptor-patterns-in-patients-with-benign-and-malignant-salivary-gland-tumors-a-distinct-role-for-ccr7
#12
Mohammad Reza Haghshenas, Mohammad Javad Ashraf, Bijan Khademi, Abbas Ghaderi, Nasrollah Erfani, Mahboobeh Razmkhah
To explore the molecular mechanisms involved in pathophysiology of malignant and benign salivary gland tumors (SGTs), we investigated main tumor-inducing chemokines and chemokine receptors, CXCL12/CXCR4/ACKR3 (CXCR7), CXCR3/CXCL10, CCR5/CCL5, CCL21/CCR7, CCL2, CCR4, CXCR5, CCR6, and CXCL8 in tumor tissues. Parotid tissues were obtained from 30 patients with malignant and benign SGTs. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to determine the mRNA expression pattern of the mentioned chemokines/chemokine receptors and immunohistochemistry (IHC) was performed to verify the expression of CCR7...
March 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840841/upregulation-of-cytokine-mrna-in-circulating-leukocytes-during-human-endotoxemia
#13
Petra Jilma-Stohlawetz, Tuende Kliegel, Irene Kantner-Schlifke, Claudia Strasser-Marsik, Florian B Mayr, Bernd Jilma
Endotoxemia induces pronounced changes in leukocyte count and enhances the release of many cytokines. However, the molecular regulation of this cytokine release is poorly characterized in humans. The time course of mRNA expression of 24 cytokines in circulating leukocytes was studied in a well-standardized model of human endotoxemia (2 ng/kg). Real-time polymerase chain reaction (RT-PCR) was used to quantify the lipopolysaccharide (LPS)-inducible mRNA levels of leukocytes from 16 healthy volunteers in a randomized, placebo-controlled trial...
March 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28840840/pulmonary-artery-hypertension-pertinent-vasomotorial-cytokines
#14
Shi-Min Yuan
Pulmonary artery hypertension is a syndrome that shows similar clinical and pathophysiological features characterized by elevated pulmonary arterial pressure and resistance. There have been a series of hypotheses trying to describe the development of pulmonary artery hypertension; however, none of them perfectly explains its pathogenesis. To highlight the pathogenesis, novel vasomotorial cytokines including hypoxia-inducible factor-1α, endothelin-1, urotensin II, Krüppel-like factor 4, calcitonin gene-related peptide, angiopoietins, and serotonin closely related to pulmonary artery hypertension are discussed...
March 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28396300/effect-of-atorvastatin-on-interleukins-and-prostaglandin-e2-in-the-kidney-of-type-1-diabetic-rats
#15
Anwar D Maraqa
The aim of the study was to evaluate a possible effect of atorvastatin on renal interleukins (ILs) and prostaglandin E2 (PGE2) in type 1 diabetic rats. Thirty-two male rats from a local Wister-derived strain were included in this prospective study and were classified into four groups. Each group consisted of eight animals: Group 1, non-diabetic negative controls; Group 2, diabetic positive controls; Group 3, non-diabetic rats receiving atorvastatin for 4 weeks; and Group 4, diabetic rats receiving atorvastatin for 4 weeks...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/28396299/plasma-cytokine-profiling-to-predict-susceptibility-to-acute-mountain-sickness
#16
Hui Lu, Rong Wang, Wenbin Li, Hua Xie, Chang Wang, Ying Hao, Yuhuan Sun, Zhengping Jia
Extensive studies have been performed on acute mountain sickness (AMS), but biomarkers predicting AMS are lacking. Presently, the mainstay methods to identify AMS biomarkers include proteomic and genetic methods at high altitudes or in hypoxic simulated chambers. In the present study, we compared plasma cytokine profiles between AMS-susceptible individuals and AMS-resistant individuals at low altitude by cytokine array analysis. In total, 75 differentially expressed cytokines were identified between AMS-susceptible individuals and AMS-resistant individuals, most involved in inflammation...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/28396298/tgf-%C3%AE-1-functional-polymorphisms-a-review
#17
Guilherme Cesar Martelossi Cebinelli, Kleber Paiva Trugilo, Stephanie Badaró Garcia, Karen Brajão de Oliveira
Transforming Growth Factor β (TGF-β) is a multifunctional cytokine that plays a role in several biological processes. TGF-β1 is the most abundantly expressed isoform, associated with susceptibility to various diseases, and several polymorphisms have been described in the TGF-β1 gene structure, and some of them have been associated with functional implications. To date, eight single-nucleotide polymorphisms (SNPs) and one deletion/insertion polymorphism have been shown to affect TGF-β1 expression (rs2317130, rs11466313, rs1800468, rs1800469, rs11466314, rs1800471, rs1800470, and rs11466316); some of these interfere with transcriptional regulation by affecting the binding of transcription factors binding, while others interfere with protein production...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/28396297/the-co-regulators-src-1-and-smrt-are-involved-in-interleukin-6-induced-androgen-receptor-activation
#18
Qi Wang, Hui Wang, Qiang Ju, Zhen Ding, Xing Ge, Qiao-Mei Shi, Ji-Long Zhou, Xiao-Long Zhou, Jin-Peng Zhang, Mei-Rong Zhang, Hong-Min Yu, Li-Chun Xu
BACKGROUND: The androgen receptor (AR) can be stimulated by interleukin-6 (IL-6) in the absence of androgens to induce prostate cancer progression. The purpose of this study was to investigate whether the co-activator steroid receptor coactivator-1 (SRC-1) and co-repressor silencing mediator for retinoid and thyroid hormone receptors (SMRT) are involved in IL-6-induced AR activation. METHODS: The effects of IL-6 on LNCaP cell proliferation were monitored using real-time cell analysis (RTCA) iCELLigence system...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/28396296/direct-effects-of-interleukin-7-on-the-function-of-human-t-cells-in-vitro
#19
Vyacheslav A Shmarov, Vladimir V Malashchenko, Maksim E Meniailo, Natalia D Gazatova, Natalia M Todosenko, Olga B Melashchenko, Andrei G Goncharov, Victor I Seledtsov
СD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in СD4-positive and in CD4-negative effector memory (CD45RA(-)CD197(-)) T cell subsets, as well as in terminally differentiated (CD45RA(+)CD197(-)) Т cells, without significantly affecting the activation status of naive (CD45RA(+)CD197(+)) and central memory (CD45RA(-)CD197(+)) T cells...
November 1, 2016: European Cytokine Network
https://www.readbyqxmd.com/read/27910812/inflammatory-markers-associated-with-abdominal-aortic-aneurysm
#20
Sarah Lindberg, Moncef Zarrouk, Jan Holst, Anders Gottsäter
PURPOSE: Inflammation with leukocytic infiltration, degradation of extracellular matrix (ECM), and depletion of vascular smooth muscle cells (VSMC) are pathological hallmarks of abdominal aortic aneurysm (AAA). The aim of this study was to further evaluate relationships between AAA and inflammatory biomarkers, interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), endothelin-1 (ET-1) and soluble urokinase-type plasminogen activator receptor (suPAR), by comparing levels in 65-year-old men with and without AAA at ultrasound screening...
September 1, 2016: European Cytokine Network
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