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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/29226457/investigation-of-in-vitro-measurable-sperm-attributes-and-their-influence-on-electroejaculated-bull-semen-with-a-fixed-time-artificial-insemination-protocol-in-australian-bos-indicus-cattle
#1
N Satake, Saa Edwards, D Tutt, M R McGowan, G B Boe-Hansen
Increasing use of fixed-time artificial insemination (FTAI) in beef cattle production has presented an opportunity for the use of fresh or chilled semen as an alternative to standard cryopreserved semen. The objective of this study was to examine in vitro sperm function and pregnancy rate of electroejaculated semen, chilled and stored for 48 hr, compared to conventionally cryopreserved semen with an optimized FTAI protocol in Brahman cattle. Semen from three Brahman bulls was collected, and aliquots were extended in either chilled (at 5°C) or frozen (LN2 ) in a Tris-egg yolk extender base with 2...
December 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29205543/deep-insemination-with-sex-sorted-cashmere-goat-sperm-processed-in-the-presence-of-antioxidants
#2
Y Qin, S Yang, J Xu, C Xia, X Li, L An, J Tian
Flow cytometrically sex-sorted sperm have been widely used for improving reproductive management in the dairy industry. However, the industrial application of this technology in other domestic species is largely limited by the lower fertility after insemination. The aim of this study was to investigate effects of antioxidant supplementation during the sex-sorting and freezing process on the quality and functions of sorted sperm from Liaoning Cashmere goats. We tested the effects of antioxidant supplementation during sex-sorting and freezing process, including ascorbic acid-2-glucoside AA-2G, glutathione, melatonin and vitamin C (VC), on the quality and functions of sex-sorted fresh and frozen-thawed sperm...
December 3, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29205523/melatonin-reduces-apoptotic-cells-sod2-and-hspb1-and-improves-the-in-vitro-production-and-quality-of-bovine-blastocysts
#3
T C Marques, E C da Silva Santos, T O Diesel, L O Leme, C F Martins, Man Dode, B G Alves, Fph Costa, E B de Oliveira, M L Gambarini
Effects of adding different concentrations of melatonin (10-7 , 10-9 and 10-11  M) to maturation (Experiment 1; Control, IVM + 10-7 , IVM + 10-9 , IVM + 10-11 ) and culture media (Experiment 2; Control, IVC + 10-7 , IVC + 10-9 , IVC + 10-11 ) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10-9  M) from Experiments 1-2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10-9 , IVC + 10-9 , IVM/IVC + 10-9 ). In Experiment 1, maturated oocytes from Control and IVM + 10-9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40...
December 3, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29194807/prevalence-of-the-amhr2-mutation-in-miniature-schnauzers-and-genetic-investigation-of-a-belgian-malinois-with-persistent-m%C3%A3-llerian-duct-syndrome
#4
M M Smit, K J Ekenstedt, K M Minor, C K Lim, Paj Leegwater, E Furrow
Persistent Müllerian duct syndrome (PMDS) is a sex-limited disorder in which males develop portions of the female reproductive tract. Important consequences of PMDS are cryptorchidism and its sequelae of infertility and increased risk of testicular cancer. Anti-Müllerian hormone (AMH) and its receptor (AMHR2) induce the regression of the Müllerian ducts in male embryos. In Miniature Schnauzer dogs, the genetic basis has been identified as an autosomal recessive nonsense mutation in AMHR2, but the allele frequency of the mutation is unknown...
December 1, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29194804/the-use-of-ultrasonography-in-the-reproductive-evaluation-of-boars
#5
R O Pinho, B S Camilo, Dma Lima, Fac Villadiego, Jcm Vergara, H H Shiomi, R E Cardoso, P S Lopes, Sef Guimarães, J D Guimarães
The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty-six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles...
December 1, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29194800/expression-of-ifnar1-and-ifnar2-in-cattle-placenta-during-early-pregnancy
#6
W Wang, R Liu, X Liang, Q Zhao, P Qu, K Yao, M Jiang, Y Luo, W Zhang, S Qing
Interferon-tau (IFNT), a type I interferon, is an antiluteolytic factor secreted by trophoderm during pregnancy. IFNT transmitted signals or stimulated the expression of some factors to build maternal recognition and keep pregnancy by binding its receptors, IFNT receptor 1(IFNAR1) and IFNT receptor 2 (IFNAR2). Up to now, the expression model and roles of IFNAR1 and IFNAR2 in placenta have not been investigated in cattle. In this study, the localization and expression of IFNAR1 and IFNAR2 in the cattle placenta at days 18-50 of pregnancy were detected by histological examination, immunofluorescence staining and real-time qPCR...
December 1, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29164721/in-vitro-studies-of-norwegian-red-bovine-semen-immobilized-and-cryopreserved-in-alginate-solid-gel-network
#7
A H Alm-Kristiansen, E R Gaustad, G Bai, F B Standerholen, G Klinkenberg, E Kommisrud, K E Waterhouse
Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time-period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital(®) technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post-thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital(®) (SV) technology, the first commercialized production line of SpermVital(®) (C) and by conventional procedure applying Biladyl(®) extender (B)...
November 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29164718/canine-prostate-specific-esterase-cpse-as-an-useful-biomarker-in-preventive-screening-programme-of-canine-prostate-cpse-threshold-value-assessment-and-its-correlation-with-ultrasonographic-prostatic-abnormalities-in-asymptomatic-dogs
#8
S Alonge, M Melandri, R Leoci, G M Lacalandra, G Aiudi
Due to the increased attention that pet-owners devote to their animals and to the improved veterinary care, investigations regarding methods to early detect prostatic disorders that might affect canine life quality have been performed. Canine prostate specific esterase (CPSE) concentration was reported to be higher in dogs suffering from prostatic diseases. This study aimed to estimate the CPSE threshold as a biomarker to early identify prostatic diseases in asymptomatic dogs. The ultrasonographic examination of the prostate was performed in 19 dogs (6-40 kg; 1-5 years) with no symptoms of prostatic diseases...
November 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29164713/importance-of-oil-overlay-for-production-of-porcine-embryos-in-vitro
#9
REVIEW
C A Martinez, E A Martinez, M A Gil
Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques...
November 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29164710/expression-profiles-of-immunity-and-reproductive-genes-during-transition-period-in-holstein-cattle
#10
A I Ateya, M S Hussein, H M Ghanem, R M Saleh, W B El-Domany, Y Y Elseady
The transition period is a critical time for dairy cows as the animal is subjected to the physiological stress accompanying parturition. Immunosuppression and health status were examined during this period in 80 Holstein cows. Blood samples were taken from each cow 3, 2 and 1 week before and after calving, and at calving (0 day). RNA was extracted and subjected to real-time PCR to determine mRNA levels for the immune-related genes TLR 2, 4, 6, 7 and β-defensin 5 in addition to the reproduction-related genes prolactin and IGF-I...
November 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29143382/the-effect-of-resveratrol-on-the-developmental-competence-of-porcine-oocytes-vitrified-at-germinal-vesicle-stage
#11
Ecs Santos, R Appeltant, T Q Dang-Nguyen, J Noguchi, H Kaneko, K Kikuchi, T Somfai
We tested the effects of resveratrol both as a pre-treatment and as a recovery treatment after warming during in vitro maturation (IVM) on the viability and developmental competence of porcine oocytes vitrified at the germinal vesicle stage. Pre-treatment before vitrification of oocytes for 3 hr with 2 μM resveratrol did not affect survival, oocyte maturation and embryo developmental competence to the blastocyst stage after parthenogenetic activation. However, supplementation of the medium with resveratrol during subsequent IVM after vitrification and warming significantly improved the ability of surviving oocytes to develop to the blastocyst stage, and this effect was observed only on vitrified, but not on non-vitrified oocytes...
November 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29143380/polo-like-kinase-1-inhibition-results-in-misaligned-chromosomes-and-aberrant-spindles-in-porcine-oocytes-during-the-first-meiotic-division
#12
Y Liao, D Lin, P Cui, B Abbasi, C Chen, Z Zhang, Y Zhang, Y Dong, R Rui, S Ju
Polo-like kinase 1 (Plk1), a type of serine/threonine protein kinase, has been implicated in various functions in the regulation of mitotic processes. However, these kinase's roles in meiotic division are not fully understood, particularly in the meiotic maturation of porcine oocytes. In this study, the expression and spatiotemporal localization of Plk1 were initially assessed in the meiotic process of pig oocytes by utilizing Western blotting with immunofluorescent staining combined with confocal microscopy imaging technique...
November 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29135047/effects-of-voltage-strength-during-electroporation-on-the-development-and-quality-of-in-vitro-produced-porcine-embryos
#13
K Nishio, F Tanihara, T-V Nguyen, T Kunihara, M Nii, M Hirata, T Takemoto, T Otoi
This study was conducted to determine suitable conditions for an experimental method in which the CRISPR/Cas9 system is introduced into in vitro-produced porcine zygotes by electroporation. In the first experiment, when putative zygotes derived from in vitro fertilization (IVF) were electroporated by either unipolar or bipolar pulses, keeping the voltage, pulse duration and pulse number fixed at 30 V/mm, 1 msec and five repeats, respectively, the rate of blastocyst formation from zygotes electroporated by bipolar pulses decreased compared to zygotes electroporated by unipolar pulses...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134714/identification-of-perivascular-and-stromal-mesenchymal-stem-progenitor-cells-in-porcine-endometrium
#14
J Wiater, M Niedziela, A Posmysz, K Wartalski, B Gajda, Z Smorąg, Z Rajfur, J Karasiński
Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134700/comparative-analysis-of-ovarian-transcriptomes-between-prolific-and-non-prolific-goat-breeds-via-high-throughput-sequencing
#15
X-D Zi, J-Y Lu, H Zhou, L Ma, W Xia, X-R Xiong, D-L Lan, X-H Wu
To increase the current understanding of the gene expression in the pre-ovulatory ovary and identify the key genes involved in the regulation of ovulation rate, we compared the transcriptomes of ovaries from the prolific Jintang black goat (JTG) and the non-prolific Tibetan goat (TBG) during the follicular phase using the Illumina RNA-Seq method. Three ovarian libraries were constructed for each breed. On average, we obtained approximately 49.2 and 45.9 million reads for each individual ovary of TBGs and JTGs, respectively, of which 79...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134697/cytological-endometritis-diagnosed-at-artificial-insemination-in-repeat-breeder-dairy-cows
#16
O Bogado Pascottini, M Hostens, G Opsomer
This study aimed to (i) assess the prevalence of cytological endometritis (CYTO) diagnosed at artificial insemination (AI); (ii) evaluate the effect of CYTO on the pregnancy outcome of the same AI sample; and (iii) determine the risk factors associated with CYTO diagnosed at AI in repeat breeder (RB) dairy cows. We analysed the productive and reproductive performances of 146 RB Holstein-Friesian cows. To obtain a CYTO sample at AI, we used the cytotape technique. Generalized mixed effect models were computed to find the risk factors associated with the pregnancy and CYTO outcome...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134695/acute-stimulation-of-a-smooth-muscle-constrictor-by-oestradiol-17%C3%AE-via-gper1-in-bovine-oviducts
#17
T Nishie, Y Kobayashi, K Kimura, K Okuda
Oviducts play roles in reproductive processes, including gametes transport, fertilization and early embryo development. Oviductal transport is controlled by various factors such as endothelins (EDNs) and nitric oxide (NO), smooth muscle contracting and relaxing factor, respectively. EDNs and NO production depend on an ovarian steroid hormone, oestradiol-17β (E2) and E2 quickly exerts their biological functions through G protein-coupled oestrogen receptor 1 (GPER1), which mediates rapid intracellular signalling...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134689/effectiveness-of-controlled-internal-drug-release-device-treatment-to-alleviate-reproductive-seasonality-in-anestrus-lactating-or-dry-barki-and-rahmani-ewes-during-non-breeding-season
#18
M Y El-Mokadem, Anm Nour El-Din, T A Ramadan, A M Rashad, T A Taha, M A Samak, M H Salem
This study aimed to evaluate the effectiveness of hormonal treatments on ovarian activity and reproductive performance in Barki and Rahmani ewes during non-breeding season. Forty-eight multiparous ewes, 24 Barki and 24 Rahmani ewes were divided into two groups, 12 lactating and 12 dry ewes for each breed. Controlled internal drug release (CIDR) device was inserted in all ewes for 14 days in conjunction with intramuscular 500 IU equine chronic gonadotrophin (eCG) at day of CIDR removal. Data were analysed using PROC MIXED of SAS for repeated measures...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29134682/differential-effects-of-follistatin-on-porcine-oocyte-competence-and-cumulus-cell-gene-expression-in-vitro
#19
Z Guo, M S Islam, D Liu, G Liu, L Lv, Y Yang, B Fu, L Wang, Z Liu, H He, H Wu
Several oocyte-derived genes/proteins are essential to early embryonic development. The expression and stability of these proteins are influenced by the autocrine/paracrine activity of factors released by oocytes and cumulus cells. This study investigated the paracrine and autocrine activity of follistatin (FS), which is secreted by oocytes and cumulus cells as part of porcine embryogenesis. Immunohistochemical (IHC) localization of follistatin was conducted on 100 randomly selected early- and late-cleaving two-cell embryos...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29119664/effects-of-body-condition-score-bcs-on-steroid-and-eicosanoid-metabolizing-enzyme-activity-in-various-mare-tissues-during-winter-anoestrus
#20
Mpt Owen, E N Ferjak, C A Cavinder, K J McCarty, K C Yankey, C G Hart, D D Burnett, Ttn Dinh, C O Lemley
The objective of this study was to determine the activity of steroid- and eicosanoid-metabolizing enzymes in horses with varying BCSs. The BCSs of twenty non-pregnant, anoestrous mares were determined prior to euthanasia, and tissue samples were collected from the liver, kidney, adrenal gland, ovary and endometrium. Cytochrome P450 1A (CYP1A), 2C (CYP2C), 3A (CYP3A) and uridine 5'-diphospho-glucuronosyltransferase (UGT) activities were determined using luminogenic substrates. The MIXED procedure of SAS was used to test the effect of BCS on enzyme activity and differences between tissues...
November 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
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