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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28109018/gonad-histology-and-serum-11-kt-profile-during-the-annual-reproductive-cycle-in-sterlet-sturgeon-adult-males-acipenser-ruthenus
#1
A Golpour, C Broquard, S Milla, H Dadras, A R Baloch, T Saito, M Pšenička
The aim of this study was to assess monthly testicular development in the cultured breeding stock of sterlet, Acipenser ruthenus, using histological and serum sex steroid changes. Testicular development in the adult male was examined monthly and showed four distinct phases including resting, pre-spawning, spawning and post-spawning. Also, seasonal changes of the testes were described according to its variations in gonadosomatic index (GSI) during different phases of testicular development. Using histology, we identified continuous spermatogenesis and asynchronous gonad development pattern in the testes of male sterlet, which shows that regulation of annual gonadal cycle is influenced by season...
January 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28109010/lack-of-effects-of-an-equine-chorionic-gonadotropin-ecg-administration-between-days-9-and-15-postpartum-on-reproductive-performance-in-a-holstein-dairy-herd
#2
M Freick, O Passarge, J Weber
Recently, it has been demonstrated that administration of equine chorionic gonadotropin (eCG) in the postpartum period in dairy cows can enhance follicle growth, reduce the interval from calving to first ovulation and increase plasma estradiol concentrations, and, thus, could enhance reproductive performance in a dairy herd when administered on day 6 postpartum. The aim of this study was to investigate the effects of a single dose of eCG between days 9 and 15 postpartum on parameters of reproductive performance in dairy cows...
January 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28109031/undifferentiated-embryonic-cell-transcription-factor-1-utf1-and-deleted-in-azoospermia-like-dazl-expression-in-the-testes-of-donkeys
#3
Y S Lee, H J Jung, M J Yoon
Putative markers for each specific germ cell stage can be a useful tool to study the fate and functions of these cells. Undifferentiated embryonic cell transcription factor 1 (UTF1) is a putative marker for undifferentiated spermatogonia in humans, rats and horses. The deleted in azoospermia-like (DAZL) protein is also expressed by differentiated spermatogonia and primary spermatocytes in several species. However, whether the expression patterns of these molecular markers are identical and applicable to donkeys remains to be elucidated...
January 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28109029/reproductive-performance-of-breeder-quails-fed-diets-supplemented-with-l-cysteine-coated-iron-oxide-nanoparticles
#4
H Mohammadi, A Farzinpour, A Vaziry
The objective of this study was to investigate the effects of L-cysteine-coated iron oxide nanoparticles on reproductive performance in breeder quails. The five treatment diets consisted of (i) negative control diet not supplemented with iron, (ii) positive control diet supplemented with 60 mg/kg of Fe3 O4 and (iii) experimental diets supplemented with 0.6, 6 and 60 mg/kg of L-cysteine-coated iron oxide nanoparticles. A total of 100 seven-day-old quail chicks were weighed and randomly placed to five groups of five replicate cages...
January 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28101935/effect-of-cholesterol-loaded-cyclodextrins-on-cryosurvival-of-dog-spermatozoa
#5
J Khan, M Z Tahir, A Khalid, A Sattar, N Ahmad
Cryopreservation affects integrity of cholesterol and phospholipids in the plasma membrane of sperm leading to decreased fertility of frozen-thawed semen. Cholesterol-loaded cyclodextrins (CLC) have been shown to improve post-thaw semen quality in various species. The aim of this study was to investigate the optimal concentration of CLC for better post-thaw semen quality in dogs. Semen collection, through digital manipulation, was conducted once a week in four adult German shepherd dogs (n = 20 ejaculates; five ejaculates/dog)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28101891/different-expression-of-leptin-and-igf1-in-the-adult-and-prepubertal-testis-in-dogs
#6
L Müller, M P Kowalewski, I M Reichler, E Kollár, O Balogh
Leptin (Lep) and insulin-like growth factor 1 (IGF1) are implicated in the regulation of testicular function, but in dogs, our knowledge is limited to the possible role of the IGF1 system in testicular tumours. In this study, we aimed to describe and compare gene expression and protein localization of Lep, IGF1 and their receptors (LepR and IGF1R, respectively) in the testis of healthy adult and prepubertal dogs. Testes were collected from sexually healthy mature (n = 7) and from 8-week-old dogs (n = 7)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28066955/dose-rates-of-antimicrobial-substances-in-boar-semen-preservation-time-to-establish-new-protocols
#7
M Schulze, M Grobbel, A Riesenbeck, S Brüning, J Schaefer, M Jung, R Grossfeld
To achieve a standardized number of spermatozoa in the final AI dose, varying amounts of extender fluid with a fixed concentration of antimicrobial substances are currently added to boar ejaculates. This practice ignores the different degrees of dilution of the antimicrobials in the end product. In calculating the final concentration of gentamicin in AI doses from 27,538 processed boar ejaculates, we demonstrated varying gentamicin concentrations in the resultant extended boar semen samples. The median concentration was 220...
January 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058771/short-communication-progressive-motility-of-frozen-thawed-canine-semen-is-highest-five-minutes-after-thawing
#8
S Karger, B Geiser, M Grau, W Heuwieser, S P Arlt
Progressive motility is usually estimated by visual inspection using a light contrast microscope at X 100 immediately after semen collection or immediately after thawing frozen semen. Standard operating procedures have never been established for this test. The objective of this experiment was to examine time-dependent changes of motility after thawing cryopreserved canine semen. Semen of 35 dogs was collected, and volume, concentration, progressive motility, morphology, membrane integrity and HOS test were evaluated...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058759/effect-of-sperm-pretreatment-with-glutathione-and-membrane-destabilizing-agents-lysolecithin-and-triton-x-100-on-the-efficiency-of-bovine-intracytoplasmic-sperm-injection
#9
F Zambrano, L Aguila, M E Arias, R Sanchez, R Felmer
Intracytoplasmic sperm injection (ICSI) is an assisted reproduction tool with several applications. Its effectiveness in bovines is lower than that in other species, mainly because of difficulties in the decondensation of the sperm nucleus after injection, and the presence of the acrosome and the plasma membrane which remain intact in this procedure. In this study, we assessed the effect of lysolecithin (LL) and Triton X-100 (TX), in combination with glutathione (GSH) as sperm pretreatments prior to ICSI. The GSH-LL and GSH-TX groups showed 0% of spermatozoa with intact membrane (SYBR 14+/PI), in comparison with the control (63...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058745/lipid-and-protein-oxidation-levels-in-spermatozoa-and-seminal-plasma-of-asian-elephants-elephas-maximus-and-their-relationship-with-semen-parameters
#10
S Satitmanwiwat, K Promthep, K Buranaamnuay, S Mahasawangkul, K Saikhun
Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058738/epigallocatechin-3-gallate-egcg-and-green-tea-polyphenols-do-not-improve-stallion-semen-parameters-during-cooling-at-4%C3%A2-c
#11
D Bucci, M Spinaci, B Mislei, B Gadani, G Rizzato, C C Love, C Tamanini, G Galeati, G Mari
Stallion semen storage for artificial insemination is mainly based on liquid cooled storage. In many stallions this technique maintains sperm quality for an extended period of time (24-72 hr) at 7°C. While this technique is commonly used in the horse industry, there can be a decline in fertility in some stallions, due to an inability of their sperm to tolerate the cool storage process. The aim of the present work was to evaluate the effect of two natural antioxidants (epigallocatechin-3-gallate (EGCG) at 20, 60 and 120 μm and green tea polyphenols, and p at ...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28058736/effect-of-mitochondrial-uncoupling-and-glycolysis-inhibition-on-ram-sperm-functionality
#12
Jda Losano, Dsr Angrimani, A Dalmazzo, B R Rui, M M Brito, C M Mendes, Gkv Kawai, C I Vannucchi, Meoa Assumpção, V H Barnabe, M Nichi
Studies have demonstrated the importance of mitochondria to sperm functionality, as the main source of ATP for cellular homoeostasis and motility. However, the role of mitochondria on sperm metabolism is still controversial. Studies indicate that, for some species, glycolysis may be the main mechanism for sperm energy production. For ram sperm, such pathway is not clear. Thus, we evaluated ram sperm in response to mitochondrial uncoupling and glycolysis inhibition aiming to assess the importance of each pathway for sperm functionality...
January 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28025854/changes-in-acetylation-of-lysine-5-on-histone-h4-in-canine-oocytes-following-in-vitro-maturation
#13
T F Motheo, D R Arnold, L C Padilha-Nakaghi, E A Pires-Buttler, A E Alves, M Apparicio, Wrr Vicente, F L Lopes
Post-translational modifications of histones, such as acetylation, are involved in regulating chromatin remodelling and gene expression. Proper in vitro maturation (IVM) of canine oocytes, for many reasons, is up to now inefficient. This study aimed to evaluate the post-translational histone H4 acetylation at lysine 5 (H4K5) in immature and post-IVM canine oocytes. Oocyte nuclear stage was assessed using Hoechst 33342 staining. Acetylation patterns were determined by indirect immunofluorescence staining of immature and post-IVM oocytes, using an antibody against the acetylated lysine 5 residue on histone 4 (H4K5ac)...
December 27, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28025851/long-term-effects-of-gnrh-agonists-on-fertility-and-behaviour
#14
S Goericke-Pesch
This review aimed to summarize the present knowledge about the effects of GnRH agonist slow-release implants (GnRH A-SRI) on fertility and behaviour in male and female dogs and cats with special focus on deslorelin. Following an initial stimulation of gonadotropin and testosterone secretion possibly associated with an improved semen quality, GnRH A-SRI induce long-term depression of fertility in male dogs and cats with, however, a large individual variation in onset and duration of efficacy especially in cats...
December 27, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28025846/prolactin-and-growth-hormone-immunoactivity-in-canine-mammary-adenomas-and-adenocarcinomas
#15
E R Bohrer, C V Löhr, M A Kutzler
It is now widely accepted in human medicine that prolactin (PRL) and growth hormone (GH) function in the mammary gland in an autocrine and paracrine manner in tumour formation. The aim of this study was to compare PRL and GH immunoactivity in canine mammary tumours submitted for histopathologic evaluation. Formalin-fixed specimens from spontaneously occurring mammary adenomas and adenocarcinomas from 24 female client-owned dogs were used. Information pertaining to the reproductive status of the patient at the time of mammary tumour diagnosis was obtained from each of the submitting veterinarians...
December 27, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28025841/regulation-of-diapause-in-carnivores
#16
J C Fenelon, P L Lefèvre, A Banerjee, B D Murphy
Embryonic diapause is an evolutionary strategy to ensure that offspring are born when maternal and environmental conditions are optimal for survival. In many species of carnivores, obligate embryonic diapause occurs in every gestation. In mustelids, the regulation of diapause and reactivation is influenced by photoperiod, which then acts to regulate the secretion of pituitary prolactin. Prolactin in turn regulates ovarian steroid function. Reciprocal embryo transplant studies indicate that this state of embryonic arrest is conferred by uterine conditions and is presumed to be due to a lack of specific factors necessary for continued development...
December 27, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27987255/assessment-of-spermatozoa-in-fertile-alpaca-vicugna-pacos-males-study-of-sperm-head-morphometry-using-a-nonautomated-digital-method-and-sperm-morphology-based-on-strict-criteria
#17
D Evangelista-Vargas, S Evangelista-Vargas, M Valdivia, A Santiani
Although computer-assisted systems for sperm morphometry and morphological analysis are important tools in the study of male fertility, their use in extensive systems in alpacas is limited by factors such as the expense of equipment and the high altitudes of the Andean region. The objectives of this study were to evaluate alpaca sperm head morphometry using a nonautomated digital method and determine the frequency of sperm abnormalities based on strict criteria for sperm morphology in fertile male alpacas. Ejaculates (n = 15) from seven alpacas were collected, and sperm smears stained with modified Papanicolaou were processed...
December 16, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27976471/coxiella-burnetii-serological-reactions-and-bacterial-shedding-in-primiparous-dairy-cows-in-an-endemically-infected-herd-impact-on-milk-yield-and-fertility
#18
M Freick, H Enbergs, J Walraph, R Diller, J Weber, A Konrath
Coxiella burnetii (C. burnetii) is the causative agent of Q fever both in humans and animals. The objectives of this study were to investigate seropositivity and bacterial shedding in heifers and primiparous cows in an endemically infected herd and to assess the effects on post-partum diseases, fertility and milk production. At the age of 9 months, 96 Holstein heifers were included. Sampling was performed reproduction-orientated: at the beginning of the study, at detection of first pregnancy, 3 weeks before expected calving date (blood serum), at parturition and after 21, 42, 100 and 150 days in milk (DIM) (blood serum, vaginal swabs and milk)...
December 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27966828/the-onset-of-puberty-in-cameroon-dwarf-goats-kept-as-pets-in-northwestern-croatia
#19
D Đuričić, S Vince, H Valpotić, I Žura Žaja, R Turk, M Lojkić, I Getz, V Berta, M Samardžija
The aim of this study was to determine onset of puberty in Cameroon Dwarf goats (CDGs) kept as pets in northwestern Croatia by determining progesterone (PGS) and insulin-like growth factor-I (IGF-I) levels in the blood by RIA methods. The first cyclic ovarian activity was estimated according to hormone profiles as determined in CDG in a moderate climate environment. Sixteen female CDG kids were kept in stables with access to pasture which provided space for exercise. The goat kids born in winter (December-January-February), spring (March-April-May), summer (June-July-August) and autumn (September-October-November) were assigned into four groups according to the season of the year of birth (n = 4 in each group)...
December 14, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27966823/oxytocin-receptors-in-dioestrous-and-anoestrous-canine-uteri
#20
T M Tamminen, L Sahlin, B Masironi, J Taponen, O Laitinen-Vapaavuori, T Katila
The aim of the study was to localize oxytocin receptors (OTR) and measure mRNA expression of OTR in the canine uterus with and without the influence of progesterone. Uterine samples were taken from nine anoestrous and eight dioestrous bitches during ovariohysterectomy. Histological changes were evaluated in haematoxylin and eosin (HE)-stained samples. Purified polyclonal antibody for OTR was used in immunohistochemistry to localize receptors in uterine layers. Relative mRNA concentration of OTR was evaluated with real-time PCR from full-thickness uterine samples taken from the middle horn and the body...
December 14, 2016: Reproduction in Domestic Animals, Zuchthygiene
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