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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28963736/expression-of-gnrh-receptor-in-the-canine-corpus-luteum-and-luteal-function-following-deslorelin-acetate-induced-puberty-delay
#1
D Kaya, A Gram, M P Kowalewski, S Schäfer-Somi, M Kuru, A Boos, S Aslan
The goals of this study were as follows: (Experiment 1) to examine the basic capability of canine corpora lutea (CL) to respond to GnRH by assessing expression of gonadotropin-releasing hormone receptor (GnRH-R) in luteal samples collected throughout the luteal lifespan from non-pregnant dogs, and (Experiment 2) to investigate the effects of pre-pubertal application of the GnRH agonist deslorelin acetate on luteal function following the first oestrus. Mature CL were collected during the mid-luteal phase (days 30-45) from treated and control bitches...
September 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28960537/effect-of-freezing-bull-semen-in-two-non-egg-yolk-extenders-on-post-thaw-sperm-quality
#2
I B Lima-Verde, A Johannisson, T Ntallaris, E Al-Essawe, Z Al-Kass, T Nongbua, F Dórea, N Lundeheim, K Kupisiewicz, A Edman, J M Morrell
Traditionally, extenders for bull semen included egg yolk or milk, but recently there has been a move to avoid material of animal origin. The aim of this study was to evaluate the effects of two commercial extenders (based on soya lecithin and liposomes) on bull sperm quality after cryopreservation. Post-thaw sperm quality was evaluated by computer-assisted sperm analysis and flow cytometric assessment of membrane integrity, chromatin integrity, mitochondrial membrane potential, production of reactive oxygen species and tyrosine phosphorylation...
September 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28960511/effect-of-oestrous-synchrony-between-embryo-donors-and-recipients-embryo-quality-and-state-on-the-pregnancy-rate-in-beef-cattle
#3
McC Rodrigues, Alm Bonotto, Dav Acosta, A A Boligon, M N Corrêa, C C Brauner
This study aimed to evaluate the effect of oestrous synchrony between donors and recipients and the embryo quality on the pregnancy rate in beef cow recipients. The experiment was performed over two years at an embryo transfer (ET) centre in Southern Brazil. Ninety Aberdeen Angus cows were subjected to superovulation (SOV) protocols, resulting in the recovery of 1,048 transferable embryos. Eleven groups were formed with intervals of 6 hr, from -30 to +30 hr, with respect to recipient versus donor oestrous detection...
September 27, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28944513/preliminary-data-on-possible-protein-markers-of-parturition-in-cows
#4
J Wawrzykowski, M Franczyk, M Hoedemaker, M Pries, B Streuff, M Kankofer
Parturition is one of the most important events in reproduction. Regardless of many studies, exact time for pregnancy termination and onset of parturition is impossible to determine. The aim of this study was to describe and to compare protein profile of plasma from healthy pregnant cows (n = 6) at following five time points: 2 weeks, 1 week before, at parturition, 1 week and 2 weeks after parturition to search for possible protein markers of parturition. Plasma samples were analysed by 1D and 2D electrophoresis, and selected spots were identified by mass spectrometry...
September 24, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28944507/peri-conceptional-under-nutrition-alters-transcriptomic-profile-in-the-endometrium-during-the-peri-implantation-period-the-study-in-domestic-pigs
#5
K Zglejc, M Martyniak, E Waszkiewicz, G Kotwica, A Franczak
Female under-nutrition during early pregnancy may affect the physiological pattern of the transcriptomic profile in the endometrium. We aimed to determine if restricted diet applied to females during peri-conceptional period, that is, from the onset of the oestrus until day nine of pregnancy, alters transcriptomic profile in the endometrium during the peri-implantation period. The restricted diet gilts were fed forage, in which the dose of proteins and energy had been reduced by 30% compared to normal diet...
September 24, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28921680/measurement-of-urinary-concentrations-of-the-mycotoxins-zearalenone-and-sterigmatocystin-as-biomarkers-of-exposure-in%C3%A2-mares
#6
M Takagi, S Uno, E Kokushi, F Sato, Mmp Wijayagunawardane, J Fink-Gremmels
Mycotoxins may affect animal health, including reproduction. Little is known about the clinical relevance of exposure of horses to contaminated feed. This study aimed at (i) monitoring the levels of the mycotoxins zearalenone (ZEN), with its metabolites α- and β-zearalenol (α- and β-ZOL), and sterigmatocystin (STC) in urine samples from thoroughbred mares in Japan and (ii) relating these findings to the potential effects on reproductive efficacy of breeding mares. Sixty-three urine samples of breeding mares from 59 breeding farms were used...
September 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891229/effect-of-different-mini-volume-colloid-centrifugation-configurations-on-flow-cytometrically-sorted-sperm-recovery-efficiency-and-quality-using-a-computer-assisted-semen-analyzer
#7
L B Ferré, J L Chitwood, C Fresno, H H Ortega, M E Kjelland, P J Ross
Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 10(6)  sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex-sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane-coated silica colloid dilutions and layering configurations during centrifugation of sex-sorted sperm was examined over an extended period of incubation time...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891218/prostate-vascular-flow-the-effect-of-the-ejaculation-on-the-power-doppler-ultrasonographic-examination
#8
S Alonge, M Melandri, L Fanciullo, G M Lacalandra, G Aiudi
Power Doppler sonography (PD) can accurately depict tissue perfusion, recognize slow flows, and is relatively angle independent. The monitoring of local blood flow by Doppler ultrasonography is helpful in differentiating prostatic physio-pathological conditions, but the recognizing of physiological variables that could affect it is crucial to apply this technique in clinical practice. This study aimed to evaluate if ejaculation affects blood flow to the prostate and to state how long this effect lasts. Serial PD examinations of prostate were performed in 18 dogs (1-5 years, 6-40 kg) immediately before (T0) and after (T1) the ejaculation, and repeated 6 (T2), 18 (T3) and 24 (T4) hours later...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891125/effect-of-cryoprotectants-and-thawing-temperatures-on-chicken-sperm-quality
#9
M Miranda, B Kulíková, J Vašíček, L Olexiková, N Iaffaldano, P Chrenek
There is need for standardization of freezing-thawing protocol for rooster semen to minimize variability among results. Therefore, we aimed to compare effect of four different permeating cryoprotectants and two thawing temperatures (37 vs. 5°C) on sperm post-thaw motility and to analyse combined effect of the best permeating cryoprotectant (P-CPA) with one of four non-permeating cryoprotectants (N-CPA) on post-thaw quality of rooster semen evaluated in vitro. Pooled semen from Ross PM3 rooster heavy line was diluted in Kobidil extender and frozen in cryoprotectant solution containing 6% dimethylacetamide, 7...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891085/lipopolysaccharide-and-cytokines-modulate-leukotriene-lt-b4-and-ltc4-production-by-porcine-endometrial-endothelial-cells
#10
J Czarzasta, K Meller, A Andronowska, B Jana
Uterine inflammatory response is mediated by inflammatory mediators including eicosanoids and cytokines produced by immune and endometrial cells. Interactions between lipopolysaccharide (LPS) and cytokines, and leukotrienes (LTs) in endothelium, important for the host defence during the inflammation, are unknown. We studied the effect of LPS, tumour necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4 and IL-10 on 5-lipooxygenase (5-LO), LTA4  hydrolase (LTAH) and LTC4 synthase (LTCS) mRNA and protein expression, LTB4 and LTC4 release from porcine endometrial endothelial cells, and cell viability...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28833663/redox-cycling-induces-spermptosis-and-necrosis-in-stallion-spermatozoa-while-the-hydroxyl-radical-oh%C3%A2-only-induces-spermptosis
#11
P Martín Muñoz, L Anel-López, J M Ortiz-Rodríguez, M Álvarez, P de Paz, C Balao da Silva, H Rodríguez Martinez, M C Gil, L Anel, F J Peña, C Ortega Ferrusola
Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2-methyl-1,4-naphthoquinone (menadione), or hydroxyl radical formation after FeSO4 exposure...
August 17, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28815751/flow-cytometry-in-spermatology-a-bright-future-ahead
#12
REVIEW
C Ortega-Ferrusola, M C Gil, H Rodríguez-Martínez, L Anel, F J Peña, P Martín-Muñoz
Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high-throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological information.The application of flow cytometry may overcome this disadvantage, allowing proteomic analysis at the single-cell level...
August 15, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804963/effect-of-housing-system-on-reproductive-behaviour-and-on-some-endocrinological-and-seminal-parameters-of-donkey-stallions
#13
A Rota, M Sgorbini, D Panzani, F Bonelli, P Baragli, N Ille, D Gatta, C Sighieri, L Casini, M M Maggiorelli, C Aurich, F Camillo
Reproductive management of male donkeys employed for artificial breeding has been poorly studied. The aim of this study was to evaluate the effect of housing system, with the animals grouped together in a paddock or kept in individual boxes, on sexual behaviour, cortisol and testosterone concentration and seminal characteristics of adult male donkeys. The study included four Amiata donkey jacks (stallions) from which ejaculates, saliva and blood were collected during two distinct 3 weeks periods, one in the group and one in the box housing system...
August 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804962/biochemical-analysis-of-uterine-fluid-for-identification-of-indicators-for-subclinical-endometritis-in-the-water-buffalo-bubalus-bubalis
#14
S C Gahlot, S Kumar, A Kumaresan, S Vairamuthu, K K Saraf, L Sreela, R K Baithalu, S S Lathwal, T K Mohanty
Alterations in biochemical constituents of uterine fluid have been suggested for diagnosis of subclinical uterine infection in the bovine. This study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. Uterine fluid samples from normal (n = 22) and subclinical endometritis (n = 18; diagnosed based on uterine cytology)-affected buffaloes were subjected to biochemical analysis. Among the different biochemical constituents estimated, urea, urea N, cholesterol, total bilirubin and alkaline phosphatase (ALP) concentrations were significantly (p < 0...
August 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804971/abortion-in-goats-by-caprine-alphaherpesvirus-1-in-spain
#15
J Gonzalez, G Passantino, A Esnal, N Cuesta, J A García Vera, L Mechelli, A Saez, J F García Marín, M Tempesta
An abortion outbreak occurred in a goat herd of Murciano-Granadina breed in Almeria Region in Spain where 80 pregnant females aborted. All bacteriological and parasitological examinations resulted negative, whereas virological investigations and real-time PCR assay showed the presence of Caprine alphaherpesvirus 1 DNA in the pathological specimens from aborted foetuses. Nucleotide sequence analysis revealed that the DNA was highly close related to the Swiss strain E-CH (99.7%) and a little less extent to the Italian BA...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804967/comparative-proteomic-analysis-of-different-developmental-stages-of-swamp-buffalo-testicular-seminiferous-tubules
#16
P-F Zhang, Y-L Huang, Q Fu, F-M Chen, Y-Q Lu, X-W Liang, M Zhang
With ageing, many protein components change markedly during mammalian spermatogenesis. Most of these proteins have yet to be characterized and verified. Here, we have employed two-dimensional electrophoresis coupled to tandem mass spectrometry to explore the different proteins from pre-pubertal, pubertal and post-pubertal swamp buffalo testicular seminiferous tubules. The results showed that 25 protein spots were differentially expressed among developmental stages, and 13 of them were successfully identified by mass spectrometry...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804928/the-comet-assay-for-detection-of-dna-damage-in-canine-sperm
#17
A F Pereira, P Borges, A Fontbonne, L Cardoso, I Gaivão, A Martins-Bessa
Sperm DNA integrity is a fundamental prerequisite in fertilization and embryo development. Among DNA integrity tests, the Comet assay is an accurate and sensitive test for the detection of sperm oxidative damage. The aim of this work was to evaluate sperm oxidative damage using the Comet assay and to study the correlation between Comet and routine assays for the evaluation of semen quality. Dogs were divided in two groups: group A (n = 6), comprising dogs with abnormal spermiogram, that is astheno-, terato- or oligoasthenoteratozoospermic (OAT); and group B (n = 8), comprising normospermic dogs...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801993/effects-of-service-number-on-conception-rate-in-japanese-black-cattle
#18
N Irikura, M Uematsu, G Kitahara, T Osawa, Y Sasaki
The conception rate (CR) of Japanese Black cattle has been decreasing since 1990. The objectives of this study were to compare CR by artificial insemination (AI) number, and to assess the interaction between AI number and possible factors related to CR in Japanese Black cattle. Records of 11,182 AI records for 6,741 heifers and 61,302 AI records for 13,139 cows on 977 farms were analysed. The average CR of heifers was 47.0%, and CR at the first, second and third AI was higher than at the fourth or subsequent AI (p < 0...
August 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801955/single-layer-centrifugation-of-fresh-dromedary-camel-semen-improves-sperm-quality-and-in-vitro-fertilization-capacity-compared-with-simple-sperm-washing
#19
C Malo, E G Crichton, J M Morrell, B S Pukazhenthi, J A Skidmore
Single layer centrifugation (SLC) through a colloid is a tool for selecting viable mammalian spermatozoa but has not been used previously for fresh dromedary camel sperm. Semen from six camels (2 ejaculates/male) was diluted 1:5 (v:v) or 1:10 (v:v) in a Tris-citrate-fructose buffer for mechanical liquefaction by gentle pipetting. Following liquefaction, semen was processed either by SLC or by centrifugation without a colloid (control). Total and progressive motilities, CASA kinematics, vitality and acrosome integrity (eosin-nigrosin) and plasma membrane integrity (Hypo-osmotic swelling test; HOST), and fertilizing ability in a heterologous assay (zona-free goat oocytes) were evaluated...
August 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801982/influence-of-practitioner-expertise-during-early-pregnancy-diagnosis-on-pregnancy-loss-rate-a-controlled-blinded-trial
#20
R Patron, I López-Helguera, F Sebastián, J-L Pesantez-Pacheco, N Pérez-Villalobos, J Vicente González Martín, O Fargas, S Astiz
A controlled field trial was conducted to assess the potential influence of practitioner inexperience during early pregnancy diagnosis with ultrasound (PD-US) on the risk of pregnancy loss. A veterinarian with more than 10 years' experience in PD-US (Vet-A) and a veterinarian with fewer than 12 months' experience at the start of the study (Vet-B) visited the same dairy farm once a week for 33 and 26 weeks, respectively. The two veterinarians did not interact with each other at any time during the study, nor did they know that their data would later be used in this study...
August 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
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