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Reproduction in Domestic Animals, Zuchthygiene

Anna Domoslawska, Slawomir Zdunczyk, Monika Franczyk, Marta Kankofer, Tomasz Janowski
The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least 3 matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods...
September 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
Mahmoud S El-Tarabany, Kh M Al-Marakby
The objectives were to evaluate the reproductive indices and survival analysis of pregnancy outcomes in multiparous repeat breeder Holstein cows (n = 557). The cows were synchronized to ovulate by Ovsynch, new controlled internal drug release device (N-CIDRsynch), and once-used CIDR device (U-CIDRsynch). The pregnancy per AI at 28 days post insemination (P/AI 28) in the N-CIDRsynch group (28.75%) was significantly (COR= 1.49; P= 0.011) greater than that reported in the Ovsynch (23.46%) and U-CIDRsynch (21.73%) groups...
September 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Raquel Patron, Irene Lopez-Helguera, Jose Luis Pesantez-Pacheco, Natividad Perez-Villalobos, Javier Heras, Juan Vicente Gonzalez, Octavi Fargas, Susana Astiz
We recorded conception rates and estimated pregnancy rates following second and later timed artificial inseminations (TAI) after hormonal resynchronization on commercial dairy farms, using the so-called G6G protocol (PGF day-0; GnRH 2, 8d; PGF 15, 16d, GnRH 17d; TAI 18d), and the 5 day Ovsynch protocol or 5DO (GnRH day 0; PGF 5, 6d; GnRH 7d; TAI 8d). In four farms both protocols were implemented in parallel, and these 1,368 second and later TAIs were used for the protocols' comparison based on logistic regression [544 TAIs in primiparous; 824 in multiparous cows; 1024 TAIs after G6G (600 TAIs in multiparous, 424 in primiparous); 344 TAIs after 5DO (224 TAIs in multiparous, 120 in primiparous); 280 TAIs during the hot season; 1088 during the cool season]...
September 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Shun Zhang, Xiang Shu, Jinji Yang, Jinyue Shi, Xiaomei Guan, Jianrong Jiang, Yingming Wei, Chan Luo, Deshun Shi, Fenghua Lu
The present study explored a suitable parthenogenetic activation (PA) procedure for rabbit oocytes and investigated the developmental potential of somatic cell nuclear transfer (SCNT) embryos using rabbit fetal fibroblasts (RFFs). The electrical activation had the optimal rate of blastocyst (14.06%) when oocytes were activated by three direct current (DC) pulses (40 V/mm, 20 μs each) followed by 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) treatment; the blastocyst rate of ionomycin (ION)+6-DMAP+CHX (12...
September 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalhães Pedrosa Rocha, Jamily Bezerra Bruno, Carlos Henrique Lobo, Marcelo Bertolini, Regiane Rodrigues Dos Santos, Marilia de Oliveira Taumaturgo, Ramon da Silva Raposo, José Ricardo de Figueiredo, Johan Smitz, Ana Paula Ribeiro Rodrigues
The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/ mice during one month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, Fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease of morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant...
September 11, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ibrahim S Abd El-Hamid
Two experiments were carried out to determine efficiency of supplementation of ram semen extender with caffeine on chilled storage and frozen capacity of spermatozoa. In the first experiment, eighty ejaculates were collected by an artificial vagina from five adult Barki rams, age 2 - 3 years and weight 45.0 ±2.0 kg throughout the experimental period (Jan to Feb, 2017). The ejaculates were pooled and diluted (1:10) with Tris-citric egg yolk extender, and were split into 5 groups. Group 1 served as control, whereas groups 2-5 were supplemented with 0...
September 11, 2018: Reproduction in Domestic Animals, Zuchthygiene
Filip Tirpak, Tomas Slanina, Marian Tomka, Radoslav Zidek, Marko Halo, Peter Ivanic, Agnieszka Gren, Grzegorz Formicki, Katarzyna Stachanczyk, Norbert Lukac, Peter Massanyi
The use of artificial insemination in cattle breeding has evolved to global extent and insemination doses are often shipped via air transport which requires strict radiation-based examinations. For the determination of effect of non-ionizing radiation (NIR), to which are beings frequently exposed due to protection of airport or cultural event security, freshly ejaculated and cryopreserved bovine spermatozoa were used as experimental model. Following radiation with hand-held metal detector in various exposition times (0, 10 seconds, 15, 30 and 60 minutes - groups FR, FR10, FR15, FR30 and FR60) the spermatozoa underwent motility and DNA fragmentation analyses...
September 7, 2018: Reproduction in Domestic Animals, Zuchthygiene
F Acuña, M F Tano de la Hoz, A O Díaz, E L Portiansky, C G Barbeito, M A Flamini
This study reports the histochemistry and the distribution of glycoconjugates (GCs) in the zona pellucida (ZP) of preantral, secondary, tertiary, polyovulatory and atretic follicles of ovaries from non-pregnant (NPr) and pregnant (Pr) females of Lagostomus maximus. GCs were studied using histochemical and lectinhistochemical methods. The viscacha ZP was positive to all the histochemical techniques. In addition, it was observed that the intensity of staining of the ZP was constant in the different follicular stages between both female groups...
September 6, 2018: Reproduction in Domestic Animals, Zuchthygiene
A Troisi, R Orlandi, M Maranesi, C Dall'Aglio, G Brecchia, F Parillo, C Boiti, M Zerani, A Polisca
In the present study, we evaluated the dynamic changes of intra-ovarian blood flow, by real time color-coded and pulsed Doppler ultrasonography, as well as the immunopresence of prostaglandin F2α (PGF2α) receptor (FP) and peripheral plasma progesterone concentrations in pseudopregnant rabbit after PGF2α treatments at either early- (4 days) and mid-luteal (9 days) stages. During the pre-treatment observation interval of one hour, the ovarian blood flows showed a fluctuating pattern. Independently of luteal stage, PGF2α administration caused a four-fold decline in the blood flow within 40 min that was, followed 50 min later by a reactive hyperemia that lasted several hours, while the resistive index showed an opposite trend...
September 6, 2018: Reproduction in Domestic Animals, Zuchthygiene
Vu Thanh Nguyen, Nguyen Hong Nguyen
Effect of long term selection for improved growth on reproductive performance of giant freshwater prawn (GFP) Macrobrachium rosenbergii has not been reported in the literature. The present study aimed to measure correlated responses in reproductive traits and to estimate genetic parameters for these characters in a GFP population selected for high growth over ten generations from 2007 to 2016. A total of 959 breeding females (offsprings of 515 sires and 810 dams) had measurements for seven reproductive traits (weight before spawn WBS; weight after spawn WAS; Fecundity FEC or number of larvae per female at hatching; first age to reach post-larvae PLAGE; zoea(e) weight ZOEAWT; larval survival during hatchery phase SURV1 and survival from PL to tagging SURV2...
September 4, 2018: Reproduction in Domestic Animals, Zuchthygiene
H T Nie, Z Y Wang, G M Zhang, F Wang
The nutritional alteration of amino acids (AAs) profile in physiological fluid was poorly characterized in livestock. After estrus synchronization, 24 ewes were randomly assigned to 2 groups based on the nutrient requirement recommended for maintenance (M): the feed supplemented group (S, 1.5×M, N=12) and feed restricted group (R, 0.5×M, N=12) on days 6-12 of their estrous cycle, which occurred shortly before ovulation. The concentration of 30 AAs in peripheral blood (PB) and follicular fluid (FF) were quantified to calculate the PB-to-FF concentration gap for each AA and determine its correlation with metabolites and hormones in PB and FF...
September 4, 2018: Reproduction in Domestic Animals, Zuchthygiene
Camila Arroyo-Salvo, Francisco Sanhueza, Fernanda Fuentes, Favián Treulén, María Elena Arias, Paulina Cabrera, Mauricio Silva, Ricardo Felmer
Conventional in vitro fertilization has not yet been implemented in the equine species. One of the main reason has been the inability to develop a culture medium and incubation conditions supporting high levels of stallion sperm capacitation and hyperactivation in vitro. Although different culture media have been used for this purpose, human tubal fluid (HTF) medium, widely used in the manipulation of human and mice gametes, has not been reported so far in stallion sperm culture. The first part of this study aimed to compare HTF and Whitten's media on different stallion sperm quality and capacitation variables...
September 4, 2018: Reproduction in Domestic Animals, Zuchthygiene
Anthony Valverde, Mónica Madrigal, Carina Caldeira, Daznia Bompart, Javier Núñez de Murga, Sandra Arnau, Carles Soler
Motility is the most widely used indicator of sperm quality. Computer Assisted Semen Analysis (CASA) allows the objective evaluation of sperm motility parameters. CASA technology is a common tool to predict semen doses in farm animal reproduction. The kinds of video-cameras used until now for image acquisition have presented limited frame rates (FR), which have a negative influence on the quality of the obtained data. The aim of the present work was to define the optimal frame rate for a correct evaluation of boar sperm motility and its subpopulation structure...
September 1, 2018: Reproduction in Domestic Animals, Zuchthygiene
M Moawad, H Hussein, M Abd El-Ghani, G Darwish, M Badr
Camel fertility faces many problems that could be solved by assisted reproductive technologies (ART). The experiment designed to explore the effect of different cryoprotectant concentrations and combinations on viability and maturation rates of vitrified-warmed camel oocytes. Ovaries collected directly after slaughtering from local abattoir and transported to laboratory in a thermo flask contain normal physiological saline. Oocytes aspirated from follicles (2 - 8 mm) in diameter and washed three times in TCM-199 then examined under stereo- microscope for selection...
September 1, 2018: Reproduction in Domestic Animals, Zuchthygiene
Jeferson Ferreira da Fonseca, Fabiana Nunes Zambrini, José Domingos Guimarães, Marcio Roberto Silva, Maria Emilia Franco Oliveira, Felipe Zandonadi Brandão, Pawel Mieczyslaw Bartlewski, Joanna Maria Gonçalves Souza-Fabjan
This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7-day estrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d-cloprostenol by latero-vulvar route seven days apart. After the second injection of d-cloprostenol, the ewes were checked for estrus (every 12 h) and then mated by fertile rams throughout the estrous period. All ewes received 37.5 μg of d-cloprostenol (latero-vulvar) and 1 mg of estradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 h before embryo flushing...
September 1, 2018: Reproduction in Domestic Animals, Zuchthygiene
P Martinez-Ros, S Astiz, E Garcia-Rosello, A Rios-Abellan, A Gonzalez-Bulnes
The present study supports that short-term (7-days) protocols based on progestagen-impregnated sponges and progesterone-loaded CIDRs are equally effective to induce ovulatory response in sheep. There were no significant differences in the onset of estrus behavior (32.0±6.0 and 33.8±4.0h after device withdrawal for sponges and CIDRs, respectively; p > .05) and preovulatory LH discharge (5.1 ± 2.1 and 5.8 ± 3.3h after onset of estrus behavior for sponges and CIDRs, respectively; p > .05). These features are similar to previously described for classical long-term (12-14 days) treatments...
September 1, 2018: Reproduction in Domestic Animals, Zuchthygiene
Thomas Markos Chouzouris, Eleni Ntovolou, Constantinos A Rekkas, Panagiotis Georgoulias, Lambrini V Athanasiou, Georgios S Amiridis
In two experiments, we studied a) the changes of LH secretion in heifers under different feeding schedules, and b) total ghrelin concentration at estrus in cows and heifers. In experiment one, synchronized heifers were allocated in 3 groups (R, regularly fed controls; F, fasted; and F-F fasted - fed). One day after the completion of the estrus induction protocol, group F and F-F animals stayed without feed for 24h; thereafter, feed was provided to R and F-F cattle; 2 hours later GnRH was administered to all animals...
September 1, 2018: Reproduction in Domestic Animals, Zuchthygiene
Annalisa Rizzo, Edmondo Ceci, Antonio Ciro Guaricci, Raffaele Luigi Sciorsci
Recently, a new molecule, kisspeptin (Kp), and in particular Kisspeptin 10 (Kp10), was implicated in stimulating the hypothalamic-pituitary-gonadal axis. The aim of this study was to evaluate circulating Kp10 levels in the early postpartum period of the dairy cow. Blood samples were collected from 40 dairy cows, at 10 (T10), 12 (T12), 14 (T14), and 16 (T16) days after calving. Progesterone (P4) levels were evaluated using ELISA, and levels of oestrogens (E2) and Kp were evaluated using a radio-immunologic method...
August 31, 2018: Reproduction in Domestic Animals, Zuchthygiene
F D Myromslien, N H Tremoen, I Andersen-Ranberg, R Fransplass, E B Stenseth, T T Zeremichael, M van Son, E Grindflek, A H Gaustad
The sperm chromatin structure assay is a method for assessment of sperm DNA fragmentation, a parameter reported to be negatively related to field fertility in several mammal species. This method calculates a DNA fragmentation index (DFI) whose high values indicate abnormal chromatin structure. In this study, running from March 2010 until June 2017, the aim was to assess sperm DFI in stored liquid extended semen from two different pig breeds, Norwegian Landrace (NL; n=693) and Norwegian Duroc (ND; n=655) and to evaluate the influence on total number of piglets born (TNB)...
August 31, 2018: Reproduction in Domestic Animals, Zuchthygiene
Dedi Wang, Xing Du, Yinxia Li, Qi-Fa Li
Follicle-stimulating hormone receptor (FSHR) is an important G protein-coupled receptor, which is required for steroidogenesis, follicular development, and female infertility. Here, we report a novel polymorphism in the 3'-UTR that strongly influences ovine FSHR mRNA decay. The partial 3'-UTR sequence of Hu sheep FSHR gene was isolated and characterized, and a polymorphism (c.2327A>G) was identified. Luciferase assay and qRT-PCR showed that c.2327A>G polymorphism in the 3'-UTR exerts a strong regulatory role in FSHR transcription...
August 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
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