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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/27905141/first-case-of-sterility-associated-with-sex-chromosomal-abnormalities-in-a-jenny
#1
J Dorado, G Anaya, M Bugno-Poniewierska, A Molina, A Mendez-Sanchez, I Ortiz, M Moreno-Millán, M Hidalgo, P Peral García, S Demyda-Peyrás
Chromosomal abnormalities are one of the main causes of genetic infertility in horses. Currently, their detection rate is rising due to the use of new diagnostic tools employing molecular markers linked to the sex chromosome pair. Despite genetic similarities, there are no previous reports of sterility associated with chromosomal abnormalities in the domestic donkey (Equus asinus). Hereby, we determined the presence of a chromosomal mosaicism in a female donkey with reproductive problems using molecular methodologies developed for horses...
December 1, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27910138/canine-and-feline-colostrum
#2
S Chastant-Maillard, C Aggouni, A Albaret, A Fournier, H Mila
Puppy and kitten survival over the first weeks is particularly dependent on colostrum, a specific secretion of the mammary gland produced during the first 2 days post-partum. Colostrum is a source of nutrients and immunoglobulins. It also contributes to the digestive tract maturation. Colostrum differentiates from milk mainly based on its concentration in immunoglobulins G: 20-30 g/L in dog colostrum, 40-50 g/L in cats' vs <1 g/L in milk. IgG concentration rapidly drops after parturition (-50% in 24 hr)...
November 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27905152/the-effect-of-cumulus-cells-on-domestic-cat-felis-catus-oocytes-during-in-vitro-maturation-and-fertilization
#3
N Sowińska, K Frankowska, A Filipczyk, A Adamaszek, K Nalik, K Fic, A Pietsch-Fulbiszewska
The aim of this study was to evaluate the effect of co-culture of denuded oocytes with cumulus cells (CC) or cumulus-oocyte complexes (COCs) on in vitro maturation (IVM) and in vitro fertilization (IVF). Immature oocytes were collected from ovaries of domestic cats following a routine ovariectomy. Oocytes were matured in vitro for 24 hr within four groups: (i) denuded oocytes (DO), (ii) DO co-cultured with CC, (iii) DO co-cultured with COC and (iv) COC as a control group. In further experiments, COCs were matured in vitro for 24 hr, and then, oocytes were randomly divided into four groups as previously described and fertilized in vitro...
November 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27905146/temporal-changes-in-serum-luteinizing-hormone-following-ovariohysterectomy-and-gonadotropin-releasing-hormone-vaccination-in-domestic-cats
#4
H L Bateman, L M Vansandt, J Newsom, W F Swanson
Measurement of circulating luteinizing hormone (LH) concentrations in cats and temporal changes following ovariohysterectomy (OHE) or possibly GnRH vaccination may be informative for assessing their fertility, contraception or sterilization status. In this study, serum LH concentrations were measured in domestic cats (n = 6) immediately prior to and up to 120 days post-OHE. Basal LH concentrations of females previously subjected to OHE (n = 4; ~1.5 years post-OHE) were compared pre- and post-vaccination with a GnRH immunocontraceptive, and to LH concentrations in intact females...
November 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27892642/new-approaches-to-non-surgical-sterilization-for-dogs-and-cats-opportunities-and-challenges
#5
Linda Rhodes
Over the last 40 years, researchers have explored methods to non-surgically suppress fertility in animals. Immunocontraception has been used to control wildlife populations but does not confer long-term immunity. The gonadotropin-releasing hormone (GnRH) agonist deslorelin, formulated as an implant to provide 6-month to 1-year suppression of fertility in male dogs, is available commercially in some countries. Neither of these approaches provide permanent sterility. A single-dose, permanent treatment would be a valuable tool in dog and cat population control...
November 28, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27892637/distribution-of-follicles-in-canine-ovarian-tissues-and-xenotransplantation-of-cryopreserved-ovarian-tissues-with-even-distribution-of-follicles
#6
I Wakasa, M Hayashi, Y Abe, H Suzuki
Ovarian follicles are not homogeneously distributed within the ovarian cortex in several species of mammals. Yet to maximize the reproducibility of experimental results of ovarian transplantation, it is essential to assess the degree of density and distribution of follicles in ovarian tissues before their transplantation. In this study, the ovarian cortex from 13 immature bitches (ten purebred and three mongrels) was sectioned into 1.0- to 1.5-mm(3) cubes, those were fixed, sectioned and stained with haematoxylin and eosin...
November 28, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27885724/identification-of-lactoferrin-and-glutamate-receptor-interacting-protein-1-in-bovine-cervical-mucus-a-putative-marker-for-oestrous-detection
#7
W Y Lee, M H Park, K W Kim, H Song, K B Kim, C S Lee, N K Kim, J K Park, B C Yang, K B Oh, G S Im, H J Chung
Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous-specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non-oestrous and controlled internal drug release (CIDR)-induced oestrous-stage mucus proteins were purified and subjected to surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI-TOF/TOF...
November 25, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27885726/cryopreservation-of-feline-oocytes-by-vitrification-using-commercial-kits-and-slush-nitrogen-technique
#8
L Fernandez-Gonzalez, K Jewgenow
Assisted reproductive techniques are a valuable tool for conservation breeding of endangered species. Cryopreservation methods are the basis of gamete banks, supporting genetic diversity preservation. Unfortunately, cryopreservation of feline oocytes is still considered an experimental technique. The aim of this study was to compare two commercial kits, with our protocol for vitrification of cat oocytes (IZW), which comprises a three-step method with ethylene glycol, DMSO, fetal calf serum, trehalose and Ficoll PM-70...
November 24, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27885720/a-retrospective-clinical-study-of-endoscopic-assisted-transcervical-insemination-in-the-bitch-with-frozen-thawed-dog-semen
#9
S J Mason
Since the conclusion of data collation from previously published work, a further 352 inseminations using frozen-thawed dog semen by endoscopic-assisted transcervical insemination (EIU) have been performed by the author. Insemination was performed on the second day in which crenulation of the anterior vagina was detected in conjunction with a progesterone concentration of >10 ng/ml. All semen samples were analysed for total number of sperm, total motility and progressive motility using computer-assisted semen analysis (CASA)...
November 24, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27878881/beneficial-effect-of-extracellular-adenosine-5-triphosphate-treatment-on-the-indochinese-leopard-panthera-pardus-delacouri-sperm-quality-after-cryopreservation
#10
P Thuwanut, W Tipkantha, B Siriaroonrat, P Comizzoli, K Chatdarong
The Indochinese leopard (Panthera pardus delacouri) population, included in CITES Appendix I, has been declining for decades. Proper gamete preservation condition is critical for breeding programme management using artificial insemination or in vitro fertilization (IVF). The present study aimed at investigating the impact of post-thawing treatment of leopard semen with extracellular adenosine 5'-triphosphate (ATPe) on sperm quality (including morphological traits and ability to fertilize an oocyte). Semen from six adult male leopards was collected by electroejaculation (one ejaculation per cat)...
November 22, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27868249/effects-of-supplemental-dietary-vitamin-c-on-quality-of-semen-from-nile-tilapia-oreochromis-niloticus-breeders
#11
Nlaf Sarmento, Eff Martins, D C Costa, W S Silva, C C Mattioli, M R Luz, R K Luz
The objective of this study was to evaluate the effects of vitamin C on growth and quality of semen from Oreochromis niloticus breeders. One hundred and sixty males were fed with different levels of vitamin C (0, 261, 599 and 942 mg/kg diet). The higher weight values were recorded for 599 (166 g) and 942 (175 g) mg of vitamin C/kg diet. Sperm motility, vigour and concentration were higher with 599 and 942 mg of vitamin C/kg diet. The semen volume, gonadosomatic index and plasma protein data from the last week showed a direct relationship with increasing levels of vitamin C...
November 20, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27864846/monochorial-diamniotic-dizygotic-twins-in-a-german-shepherd-dog-a-case-report
#12
C Urhausen, K Wolf, A Beineke, C Dierks, M Schmicke, A Einspanier, A R Günzel-Apel
A 6.5-year-old clinically healthy German Shepherd Dog with regular oestrous cycles of 6 months was presented for pregnancy diagnosis on day 38 after ovulation (p.ov.). Ultrasonography revealed three individual placental sites in progressed resorption and two vital adequately developed foetuses sharing a joint placenta. On days 41 and 48 p.ov., sonographic signs indicated normal development of both foetuses, but on day 52 p.ov., both foetuses were found to be dead. A caesarean section was performed the same day...
November 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27859771/engineering-a-gene-silencing-viral-construct-that-targets-the-cat-hypothalamus-to-induce-permanent-sterility-an-update
#13
G A Dissen, K Adachi, A Lomniczi, T Chatkupt, B L Davidson, H Nakai, S R Ojeda
The intent of this contribution is to provide an update of the progress we have made towards developing a method/treatment to permanently sterilize cats. Our approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction and a virus-based gene therapy system intended to deliver RNAi selectively to the hypothalamus (where these genes are expressed) via the systemic administration of modified viruses. We selected the hypothalamus because it contains neurons expressing Kiss1 and Tac3, two genes essential for reproduction and fertility...
November 17, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862454/effect-of-testicular-tissue-lysate-on-developmental-competence-of-porcine-oocytes-matured-and-fertilized-in-vitro
#14
A K Singh, S Naskar, B Saikia, Y Vashi, S Gupta, S Banik, M K Tamuli, V Pande, D K Sarma, S K Dhara
The objective of the present study was to investigate the effect of testicular tissue lysate (TTL) on developmental competence of germinal vesicle (GV) stage porcine oocytes. Two types of TTL were prepared through repeated freeze-thaw in liquid nitrogen, one from whole testicular tissue (wTTL) and other from either of four different sections of testes, namely just beneath the tunica albuginea (TA), from the transitional area between the seminiferous cord/tubules and the mediastinum testis (TR) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin)...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862411/natural-and-artificial-hyperimmune-solutions-impact-on-health-in-puppies
#15
H Mila, A Grellet, C Mariani, A Feugier, B Guard, J Suchodolski, J Steiner, S Chastant-Maillard
Colostrum and milk are complex mammary secretions providing the puppy with many nutritional and immunological factors, which play a crucial role for its correct development and survival. In the case of colostrum and/or milk intake deficiency, puppies are at increased risk of infectious diseases. This work reviews the various nutritional hyperimmune supplementations proposed to provide a passive immune protection and to positively impact puppies' health. Some strategies rely on canine immunoglobulins: canine colostrum banking and canine serum/plasma supplementation...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862405/decidualization-of-the-canine-uterus-from-early-until-late-gestational-in-vivo-morphological-observations-and-functional-characterization-of-immortalized-canine-uterine-stromal-cell-lines
#16
F R Graubner, I M Reichler, N A Rahman, R Payan-Carreira, A Boos, M P Kowalewski
The apparent lack of classical mechanisms for maternal recognition of pregnancy is one of the most intriguing features of canine reproduction. Consequently, similar levels of circulating luteal steroids are observed in pregnant and non-pregnant dogs. However, the early pre-implantation canine embryo locally modulates uterine responses to its presence, facilitating the successful onset of pregnancy. As a part of this interaction, the canine uterus undergoes a species-specific decidualization. Maternal stroma-derived decidual cells develop, the only cells of the canine placenta expressing progesterone receptor (PGR)...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862396/research-on-reproduction-is-essential-for-captive-breeding-of-endangered-carnivore-species
#17
K Jewgenow, B C Braun, M Dehnhard, J Zahmel, F Goeritz
Assisted reproductive technology (ART) has great potential for conservation, but its successful application in captive breeding programmes of endangered species is often compromised by limited background on species' biology. Although carnivore species benefit from knowledge obtained in domesticated species (dogs, cats and ferrets), the focus of research is different. In pet animals, research in reproduction has mainly been focused on ovarian function and contraception, although substantial progress has also been made in the field of in vitro embryo production, transgenic embryos and cloning to aid relevant medical models...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862395/the-nuclear-and-developmental-competence-of-cumulus-oocyte-complexes-is-enhanced-by-three-dimensional-coculture-with-conspecific-denuded-oocytes-during-in-vitro-maturation-in-the-domestic-cat-model
#18
M G Morselli, G C Luvoni, P Comizzoli
The objective of the study was to assess the efficacy of coculture with conspecific cumulus-denuded oocytes (CDOs) during in vitro maturation in a three-dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus-oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of in vitro maturation, before assessing the meiotic progression. In Experiment II, the in vitro fertilization of COCs and CDOs was carried out with chilled epididymal spermatozoa and the presumptive zygotes were cultured in vitro separately for 7 days in 3D microcapsules before assesment of embryonic development...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27862374/safety-and-effectiveness-of-a-single-and-repeat-intramuscular-injection-of-a-gnrh-vaccine-gonacon%C3%A2-in-adult-female-domestic-cats
#19
L M Vansandt, M A Kutzler, A E Fischer, K N Morris, W F Swanson
Sterilization is a key strategy to reduce the number of domestic cats entering and killed in shelters each year. However, surgical sterilization is expensive and labour-intensive and cannot fully address the 70 million free-roaming cats estimated to exist in the United States. GonaCon™ is a gonadotropin-releasing hormone vaccine originally developed for use as a wildlife immunocontraceptive. An earlier formulation was tested in domestic cats and found to be safe and effective for long-term contraception. However, the current Environmental Protection Agency (EPA)-registered formulation consists of a different antigen-carrier protein and increased antigen concentration and has never been tested in cats...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27859822/expression-of-steroidogenic-enzymes-and-steroid-receptors-in-foetal-gonads-of-domestic-cat-sex-similarities-and-differences
#20
B C Braun, K Jewgenow
Foetal gonads already produce steroid hormones and by this influence the further development of external and internal genitalia as well as of the brain. Beside this, foetal gonads themselves can be influenced by foetal or maternal hormones. The time course of foetal gonadal development can differ between species. As knowledge on processes in domestic cats is very limited, the steroidogenic enzyme expressions as well as these of steroid receptors were analysed in foetal gonads of domestic cats. We investigated a period from beginning of the second half of pregnancy to the beginning of the third trimester; a phase, where also gonadal development proceeds...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
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