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Reproduction in Domestic Animals, Zuchthygiene

W Tipkantha, P Thuwanut, B Siriaroonrat, P Comizzoli, K Chatdarong
Clouded leopards (Neofelis nebulosa) produced high proportion of abnormal spermatozoa (mainly tail defects) that can limit sperm movement and conception. The study aimed to better identify the origin of those defects using a demembranation approach. Ejaculates (1-2 ejaculations/male; n = 9) were allocated to simple washing (control; resulting in 11.7% ± 1.9% coiled tails) and processed through colloid centrifugation to reduce the number of sperm with tail defects (treatment, resulting in 5.9% ± 0.9% coiled tails)...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
B S Holst
During the last decade, analysis of anti-Müllerian hormone (AMH), highly conserved between mammalian species, has contributed to new information in reproductive endocrinology, due to clinically available diagnostic assays. AMH is produced solely in the gonads, in the Sertoli cells of testes and granulosa cells of the ovary, and thus offers possibilities to diagnose physiologic and pathologic conditions involving these organs. This article reviews indications for AMH analysis in cats and dogs, including diagnosing the presence of gonads, and granulosa or Sertoli cell tumours...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
C L Ackermann, C S Asa, R Krisher, K Bauman, S Casey, M D Lopes
Cryopreservation of gametes is an important tool to preserve fertility, but for most species, including domestic dogs, data regarding ovarian tissue cryopreservation are limited. We aimed to evaluate the follicular and tissue viability and follicular growth after in vitro culture of domestic dog ovarian cortical slices cryopreserved by vitrification. Ovarian cortex was obtained from ten pairs of ovaries from domestic dogs using two methods (A and B), one for each ovary from the same bitch. At least four slices for each method were obtained from each ovary, one was processed for histology and the other three were vitrified...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
L Mouttham, P Comizzoli
Ovarian tissue cryopreservation followed by tissue culture is a promising approach to preserving the fertility of biomedical models and endangered species. The objective of this study was to investigate the impact of exposure time to vitrification solution and presence of sucrose using different exposure temperatures and base media on intra-ovarian follicle integrity. Peripubertal ovarian cortical pieces were obtained by isolating the cortex and dissecting it into 1 × 1 × 0.2 mm(3) pieces. The cortical pieces were then exposed to equilibration solution and then vitrification solutions (VS) in one of the conditions mentioned above, plunged directly into liquid nitrogen and stored for ≥24 hr in liquid nitrogen...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
F K Hollinshead, C Walker, D W Hanlon
Anti-Müllerian hormone (AMH) is a reliable endocrine marker of ovarian reserve in many species with extensive literature in both humans and cattle. However, there are no known hormonal predictors of ovarian reserve and potential reproductive performance in the bitch. A prospective cohort study was performed involving 155 intact bitches of various ages (range 1.2-7.6 years) and breeds that were presented for routine breeding management over a one-year period. All bitches were artificially inseminated with frozen or fresh semen using the transcervical insemination (TCI) technique...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
M Olaciregui, L Gil
Cryopreservation has been routinely used to preserve sperm of human and different animal species. However, frozen sperm storage for a long time brings many inconveniences because of liquid nitrogen. Many attempts have been made to overcome the disadvantages of the current cryopreservation method. Freeze-drying has been proposed as alternative method for sperm preservation to achieve the ability to store sperm doses indefinitely at ambient temperature or in ordinary refrigerators. At present, it has been reported successfully sperm freeze-drying on many animal species including canine and feline...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
G C Luvoni, M G Morselli
The hidden treasure represented by epididymal spermatozoa has great potential in the current reproductive technologies in dogs. In case of azoospermia or when a donor male accidentally dies or undergoes orchiectomy, the retrieval of epididymal spermatozoa opens new possibilities to generate progeny. Spermatozoa can be collected by different techniques from ex vivo or in vivo testicles and can be cryopreserved for a future use. Freeze tolerance of canine epididymal spermatozoa seems lower than that of ejaculated spermatozoa; however, puppies were born after artificial insemination with frozen epididymal semen, other than with fresh and chilled...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
M S Aramli, R M Nazari, S Aramli, H A Nouri
In this study, motility and oxidative-antioxidant capacity (thiobarbituric acid-reactive substance level [TBARS], superoxide dismutase [SOD], catalase [CAT] and glutathione reductase [GR]) of beluga sturgeon (Huso huso) sperm, stored for 6 days at -80°C, were evaluated. After 2 days of storage, sperm motility was significantly decreased (no motile sperm were observed after 6 days of storage; p < .05), while TBARS and SOD values were significantly increased (p < .05). CAT and GR activities did not show significant changes among storage times (p > ...
October 17, 2016: Reproduction in Domestic Animals, Zuchthygiene
M Eslami, H Ghasemiyan, E Zadeh Hashem
The purpose of the present experiment was to investigate the protective effects of palmitoleate on the quality of ram semen during low temperature liquid storage. Ejaculates were collected using the artificial vagina from four Qezel rams twice a week. Ejaculates were pooled, diluted with Tris-egg yolk extender without palmitoleate (control) or supplemented with 0.125 (P 0.125), 0.25 (P 0.25), 0.5 (P 0.5) and 1 (P 1) mM palmitoleate at a final concentration of 500 × 10(6) spermatozoa/ml. Total motility and forward progressive motility (FPM) as well as other spermatozoa kinematics were evaluated by computer-assisted sperm analysis...
October 4, 2016: Reproduction in Domestic Animals, Zuchthygiene
J-T Kang, J Ryu, B Cho, E-J Lee, Y-J Yun, S Ahn, J Lee, D-Y Ji, K Lee, K-W Park
Pigs are an attractive animal model to study the progression of cancer because of their anatomical and physiological similarities to human. However, the use of pig models for cancer research has been limited by availability of genetically engineered pigs which can recapitulate human cancer progression. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, specific CRISPR/Cas9 systems were used to target RUNX3, a known tumour suppressor gene, to generate a pig model that can induce gastric cancer in human...
October 1, 2016: Reproduction in Domestic Animals, Zuchthygiene
E Baroncello, M L Bernardi, A D Kummer, I Wentz, F P Bortolozzo
Fixed-time post-cervical artificial insemination (FTAI) drastically reduces labour requirements and increases the use of boars with higher genetic merit. This study evaluated the efficiency of eCG administration combined with/without the GnRH agonist buserelin for the induction and synchronization of ovulation in weaned sows submitted to FTAI. The sows were allocated into three groups. In the control group, the first artificial insemination was performed at the onset of oestrus and repeated every 24 hr. In the eCG+GnRH group, sows received 600 IU eCG at weaning and buserelin (10 μg) after 86-89 hr of eCG, and in the GnRH group, sows received only buserelin after 86-89 hr of weaning...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
A Mishra, I J Reddy, Psp Gupta, S Mondal
The objective of this study was to find out the impact of L-carnitine (10 mM) on developmental regulation of preimplantation sheep embryos cultured in vitro when supplemented in maturation medium and post-fertilization medium separately. Subsequent objective was to observe the L-carnitine-mediated alteration in expression of apoptotic genes (Bcl2, Bax, Casp3 and PCNA) in sheep oocytes and developing embryos produced in vitro. Oocytes matured with L-carnitine showed significantly (p < .05) higher cleavage (67...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
M G Morselli, S Canziani, D Vigo, G C Luvoni
In the case of high valuable individuals with very precious genetic material, widening the genetic pool including gametes with poor morphological characteristics, as cumulus-denuded oocytes (CDOs), could be an option. To improve the in vitro culture of low-competence feline CDOs, an enriched three-dimensional (3D) system in association with competent cumulus-oocyte complexes (COCs) was developed. For this purpose, domestic cat CDOs were cultured with or without companion COCs in the 3D barium alginate microcapsules...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
M A Lagares, R Ecco, Nrs Martins, Ljc Lara, Jsr Rocha, Dar Vilela, V M Barbosa, P F Mantovani, Jfv Braga, I S Preis, V A Gheller, P C Cardeal, N C Baião
The objective of this study was to detect the reasons of rooster's fertility decrease at 50 weeks of age. Therefore, the reproductive system of broiler breeder roosters was laparoscopic, macroscopic and histopathology evaluated, and a comparison of the anatomical aspect with the sperm analysis and birds' age was realized. Cobb roosters (n = 59) were distributed into two groups (30 and 50 weeks). Evaluations were performed with laparoscopy, macroscopy and histopathology, and seminal quality, blood serum testosterone concentration and weight were also determined...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
Fca Sousa, G T Pessoa, L S Moura, J R Araújo, Rps Rodrigues, Maps Barbosa, A N Diniz, A B Souza, E G Silva, L U Lucena, M P Sanches, O F Silva-Filho, P C Guerra, J M Sousa, W C Neves, F R Alves
The objective of this study was to define the patterns of organogenesis and foetal haemodynamics during the normal gestation of healthy agoutis (Dasyprocta prymnolopha) kept in captivity. Thirty pregnant agoutis that ranged in size from small to medium and weighed between 2.5 and 3 kg underwent B-mode and Doppler ultrasonography for the biometric evaluation of the foetal organs. The foetal aortic blood flow proved to be predominantly systolic, and the measured flow velocity was 78.89 ± 2.95 cm/s, with a maximum pressure gradient of 2...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
C E Fedorka, K E Scoggin, E M Woodward, E L Squires, B A Ball, Mht Troedsson
In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating-induced endometritis PMIE Select seminal plasma proteins cysteine-rich secretory protein-3 (CRISP-3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP-3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding...
September 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
A Ntemka, G Tsousis, C Brozos, E Kiossis, C M Boscos, I A Tsakmakidis
The objective of this study was to investigate the quality of frozen-thawed semen from different bull breeds. Commercial frozen-thawed bull semen samples (26 per breed, 130 totally) of five breeds (Holstein [Η], Brown Swiss [BS], Limousin [L], Belgian Blue [BB], Blonde d' Aquitaine [BA]) were used. After thawing, each semen sample was subjected to thermal resistance test (TR) for 0.5 and 1 hr at 38°C and hypo-osmotic swelling test (HOST) for 1 hr at 150 mOsm at 37°C. Additionally, all samples were evaluated at times 0 hr (thawing), 0...
September 25, 2016: Reproduction in Domestic Animals, Zuchthygiene
B Li, H Luo, Q Weng, S Wang, Z Pan, Z Xie, W Wu, H Liu, Q Li
FK506-binding protein 6 (FKBP6) is essential for meiosis during mammalian spermatogenesis. However, the molecular regulation of FKBP6 during spermatogenesis remains unclear. In the present study, we performed molecular characterization of the meiosis-specific gene FKBP6 in yak testes. Yak FKBP6 encodes a polypeptide of 295 amino acid residues with an FK506-binding domain (FKBP_C) and three tetratricopeptide repeat domains. The methylation level of the FKBP6 promoter in testes was significantly higher in cattle-yak with male sterility than in yak, and the FKBP6 promoter was methylated in liver tissues in which FKBP6 is not expressed...
September 22, 2016: Reproduction in Domestic Animals, Zuchthygiene
I R Brito, G M Silva, A D Sales, C H Lobo, G Q Rodrigues, R F Sousa, Aaa Moura, Cem Calderón, M Bertolini, C C Campello, J Smitz, J R Figueiredo
This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In a first experiment, follicles were encapsulated as a single unit per bead and cultured singly or in groups or with five follicles in the same alginate (ALG) bead for 18 days. In a subsequent experiment, the "five follicles per bead" design was chosen to culture in ALG, fibrin-alginate (FA) or hyaluronate (HA) for 18 days. In a third experiment, we chose the five follicles per bead in FA to culture for 30 days...
September 21, 2016: Reproduction in Domestic Animals, Zuchthygiene
B Merlo, E Iacono, D Bucci, M Spinaci, G Galeati, G Mari
In vitro embryo production in the horse is still not as efficient as in other species. Oxidative stress negatively affects oocyte and embryo culture. To attenuate/minimize the oxidative stress, antioxidants such as low-molecular thiol compounds can be added to culture media. Beta-mercaptoethanol (BME) has been shown to improve maturation and embryo development in different species. The aim of this study was to investigate whether the addition to maturation medium of BME at common (0.1 mM) and high (0.7 mM) concentration could improve oocyte maturation also in the horse...
September 21, 2016: Reproduction in Domestic Animals, Zuchthygiene
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