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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28815751/flow-cytometry-in-spermatology-a-bright-future-ahead
#1
REVIEW
C Ortega-Ferrusola, M C Gil, H Rodríguez-Martínez, L Anel, F J Peña, P Martín-Muñoz
Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high-throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological information.The application of flow cytometry may overcome this disadvantage, allowing proteomic analysis at the single-cell level...
August 15, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804963/effect-of-housing-system-on-reproductive-behaviour-and-on-some-endocrinological-and-seminal-parameters-of-donkey-stallions
#2
A Rota, M Sgorbini, D Panzani, F Bonelli, P Baragli, N Ille, D Gatta, C Sighieri, L Casini, M M Maggiorelli, C Aurich, F Camillo
Reproductive management of male donkeys employed for artificial breeding has been poorly studied. The aim of this study was to evaluate the effect of housing system, with the animals grouped together in a paddock or kept in individual boxes, on sexual behaviour, cortisol and testosterone concentration and seminal characteristics of adult male donkeys. The study included four Amiata donkey jacks (stallions) from which ejaculates, saliva and blood were collected during two distinct 3 weeks periods, one in the group and one in the box housing system...
August 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804962/biochemical-analysis-of-uterine-fluid-for-identification-of-indicators-for-subclinical-endometritis-in-the-water-buffalo-bubalus-bubalis
#3
S C Gahlot, S Kumar, A Kumaresan, S Vairamuthu, K K Saraf, L Sreela, R K Baithalu, S S Lathwal, T K Mohanty
Alterations in biochemical constituents of uterine fluid have been suggested for diagnosis of subclinical uterine infection in the bovine. This study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. Uterine fluid samples from normal (n = 22) and subclinical endometritis (n = 18; diagnosed based on uterine cytology)-affected buffaloes were subjected to biochemical analysis. Among the different biochemical constituents estimated, urea, urea N, cholesterol, total bilirubin and alkaline phosphatase (ALP) concentrations were significantly (p < 0...
August 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804971/abortion-in-goats-by-caprine-alphaherpesvirus-1-in-spain
#4
J Gonzalez, G Passantino, A Esnal, N Cuesta, J A García Vera, L Mechelli, A Saez, J F García Marín, M Tempesta
An abortion outbreak occurred in a goat herd of Murciano-Granadina breed in Almeria Region in Spain where 80 pregnant females aborted. All bacteriological and parasitological examinations resulted negative, whereas virological investigations and real-time PCR assay showed the presence of Caprine alphaherpesvirus 1 DNA in the pathological specimens from aborted foetuses. Nucleotide sequence analysis revealed that the DNA was highly close related to the Swiss strain E-CH (99.7%) and a little less extent to the Italian BA...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804967/comparative-proteomic-analysis-of-different-developmental-stages-of-swamp-buffalo-testicular-seminiferous-tubules
#5
P-F Zhang, Y-L Huang, Q Fu, F-M Chen, Y-Q Lu, X-W Liang, M Zhang
With ageing, many protein components change markedly during mammalian spermatogenesis. Most of these proteins have yet to be characterized and verified. Here, we have employed two-dimensional electrophoresis coupled to tandem mass spectrometry to explore the different proteins from pre-pubertal, pubertal and post-pubertal swamp buffalo testicular seminiferous tubules. The results showed that 25 protein spots were differentially expressed among developmental stages, and 13 of them were successfully identified by mass spectrometry...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804928/the-comet-assay-for-detection-of-dna-damage-in-canine-sperm
#6
A F Pereira, P Borges, A Fontbonne, L Cardoso, I Gaivão, A Martins-Bessa
Sperm DNA integrity is a fundamental prerequisite in fertilization and embryo development. Among DNA integrity tests, the Comet assay is an accurate and sensitive test for the detection of sperm oxidative damage. The aim of this work was to evaluate sperm oxidative damage using the Comet assay and to study the correlation between Comet and routine assays for the evaluation of semen quality. Dogs were divided in two groups: group A (n = 6), comprising dogs with abnormal spermiogram, that is astheno-, terato- or oligoasthenoteratozoospermic (OAT); and group B (n = 8), comprising normospermic dogs...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801993/effects-of-service-number-on-conception-rate-in-japanese-black-cattle
#7
N Irikura, M Uematsu, G Kitahara, T Osawa, Y Sasaki
The conception rate (CR) of Japanese Black cattle has been decreasing since 1990. The objectives of this study were to compare CR by artificial insemination (AI) number, and to assess the interaction between AI number and possible factors related to CR in Japanese Black cattle. Records of 11,182 AI records for 6,741 heifers and 61,302 AI records for 13,139 cows on 977 farms were analysed. The average CR of heifers was 47.0%, and CR at the first, second and third AI was higher than at the fourth or subsequent AI (p < 0...
August 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801955/single-layer-centrifugation-of-fresh-dromedary-camel-semen-improves-sperm-quality-and-in-vitro-fertilization-capacity-compared-with-simple-sperm-washing
#8
C Malo, E G Crichton, J M Morrell, B S Pukazhenthi, J A Skidmore
Single layer centrifugation (SLC) through a colloid is a tool for selecting viable mammalian spermatozoa but has not been used previously for fresh dromedary camel sperm. Semen from six camels (2 ejaculates/male) was diluted 1:5 (v:v) or 1:10 (v:v) in a Tris-citrate-fructose buffer for mechanical liquefaction by gentle pipetting. Following liquefaction, semen was processed either by SLC or by centrifugation without a colloid (control). Total and progressive motilities, CASA kinematics, vitality and acrosome integrity (eosin-nigrosin) and plasma membrane integrity (Hypo-osmotic swelling test; HOST), and fertilizing ability in a heterologous assay (zona-free goat oocytes) were evaluated...
August 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28801982/influence-of-practitioner-expertise-during-early-pregnancy-diagnosis-on-pregnancy-loss-rate-a-controlled-blinded-trial
#9
R Patron, I López-Helguera, F Sebastián, J-L Pesantez-Pacheco, N Pérez-Villalobos, J Vicente González Martín, O Fargas, S Astiz
A controlled field trial was conducted to assess the potential influence of practitioner inexperience during early pregnancy diagnosis with ultrasound (PD-US) on the risk of pregnancy loss. A veterinarian with more than 10 years' experience in PD-US (Vet-A) and a veterinarian with fewer than 12 months' experience at the start of the study (Vet-B) visited the same dairy farm once a week for 33 and 26 weeks, respectively. The two veterinarians did not interact with each other at any time during the study, nor did they know that their data would later be used in this study...
August 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28758258/dominant-follicles-development-and-estradiol-17%C3%AE-concentrations-in-non-ovulating-and-ovulating-post-partum-bulgarian-murrah-buffaloes
#10
S Yotov, A Atanasov
This study was designed to evaluate the dominant follicles development and the estradiol-17β concentrations in non-ovulating and ovulating post-partum buffaloes. Sixteen Bulgarian Murrah buffaloes were submitted to transrectal ultrasonographic examination from the 1st post-partum day until day 50, 3 days apart. The follicular diameter of the different categories of follicles and the ovulations was recorded. The animals were allocated into two groups: I (n = 6) non-ovulating and II (n = 10) ovulating buffaloes...
July 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28758255/leucemia-inhibitory-factor-investigating-the-time-dependent-effect-on-viability-of-vitrified-bovine-embryos
#11
A Kocyigit, M Cevik
Leucemia inhibitory factor (LIF) is involved in various reproductive processes, including sperm development, regulation of ovulation, as well as blastocyst formation, hatching and implantation in embryos. Moreover, LIF has also been shown significantly to enhance the blastocyst formation rates of bovine embryos, a finding that remains controversial. Our purpose was to investigate time-dependent effect of LIF on bovine embryo culture, especially in terms of addition timing. Presumptive zygotes were cultured in five different groups...
July 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28758253/mir-182-selectively-targets-hoxa10-in-goat-endometrial-epithelium-cells-in-vitro
#12
L Zhang, X-R Liu, J-Z Liu, Y-X Song, Z-Q Zhou, B-Y Cao
Proper HOXA10 expression was essential for endometrial receptivity what was crucial for successful embryo implantation in mammalian. This study confirmed that miR-182 regulated the expression levels of HOXA10 by binding to its 3' UTR, selectively downregulated HOXA10 in goat endometrial epithelium cells (gEECs) but not stromal cell (gESCs) in vitro. However, HOXA10 and miR-182 both up-expressed in the goat endometrium at gestational day 15 (D15) compared with gestational day 5 (D5), suggesting that there were some other factors regulated the expression of HOXA10 during the development of goat endometrium in vivo...
July 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28755420/cytobrush-a-tool-for-sequential-evaluation-of-gene-expression-in-bovine-endometrium
#13
B Cardoso, M L Oliveira, G Pugliesi, Eos Batista, M Binelli
Aims were to (i) compare specific transcript abundance between endometrial samples collected by transcervical biopsy and cytobrush and (ii) measure the abundance of endometrial transcripts involved in PGF2α synthesis in samples collected by cytobrush. In Experiment 1, endometrial samples were taken transcervically by cytobrush and biopsy 10 days after ovulation. Compared to biopsy samples, abundance of transcripts for MSTN, AKR1C4 and PGR was similar, VIM, FLT1 and PTGES was lower (p < .05) and KRT18 and CD3D was greater in cytobrush samples (p < ...
July 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28744919/differential-expression-of-insulin-like-growth-factor-family-members-in-immature-cumulus-oocyte-complexes-from-dairy-cows-with-different-genotypes
#14
A C Lopes, M P Palhão, Cac Fernandes, M J Sudano, Acs Castilho, E S Caixeta
It has been evident the improvement of in vitro embryo production (IVEP) in dairy cows. Nevertheless, it is known that differences in the number and quality of oocytes between taurine and zebu females impact the efficiency and economic viability of IVEP. As the insulin-like growth factor (IGF) system is related to follicular and oocyte development, we aimed to quantify mRNA abundance of IGF system members and pregnancy-associated plasma protein-A (PAPPA) in the cumulus-oocyte complexes (COCs) of Gir, 1/2 Holstein × 1/2 Gir and Holstein cows...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28741737/effect-of-exogenous-progesterone-administration-on-luteal-sensitivity-to-pgf-during-the-early-development-of-the-corpus-luteum-in-mares-and-cows
#15
A Garcia-Muñoz, X Valldecabres-Torres, J R Newcombe, J Cuervo-Arango, E Garcia-Rosello
The objective of this study was to determine the effect of exogenous progesterone administration at ovulation and during the early development of the CL, on its future sensitivity to a single administration of PGF2a in mares and cows. Horse Retrospective reproductive data from an equine clinic in the UK during three breeding seasons were used. Mares were divided into: control group, cycles with single ovulations; double ovulation group cycles with asynchronous double ovulations; and PRID group: cycles with single ovulations and treatment with intravaginal progesterone device (CIDR) immediately after the ovulation...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28741693/antioxidants-protect-proteins-anchorage-to-the-bilayer-by-improving-plasma-membrane-integrity-of-ram-spermatozoa-during-liquid-preservation-in-a-soya-lecithin-based-diluent
#16
R K Paul, D Kumar, Smk Naqvi
Antioxidants are known to prevent the reactive oxygen species (ROS)-mediated peroxidative damage to the membrane lipids during hypothermic storage of mammalian spermatozoa. We hypothesized here that ROS also affect the lipid-protein interactions, thereby diminishing the membrane's integrity and proteins' anchorage to the bilayer. Antioxidants prevent these damages by scavenging the ROS. Ejaculates from Patanwadi rams were pooled after subjective evaluation and centrifuged using Percoll(®) . Sperm pellet was resuspended in soya lecithin-Tris-fructose diluent (400 × 10(6)  cells/ml) containing either antioxidants (100 IU/ml catalase + 10 mM reduced glutathione) or no antioxidant...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28731219/daylight-effect-on-melatonin-secretion-in-adult-female-guanacos-lama-guanicoe
#17
J L Riveros, L M Correa, G Schuler
The wild South American camelids developed a strategy of seasonal reproduction during spring and summer with singleton birth. The photoperiod is one of the factors that may modulate this seasonality where light would be translated into a hormonal signal. This study evaluated the influence of changes in daily light intensity on melatonin concentration in captive guanacos under a long-day photoperiod (16 hr light/8 hr dark; 33 '28'S). Mean melatonin concentration was 28.3 ± 20.3 pg/ml, with a maximum of 52...
July 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695674/foetal-pulmonary-maturity-in-dogs-estimated-from-bubble-tests-in-amniotic-fluid-obtained-via-amniocentesis
#18
T Bonte, A Del Carro, J Paquette, A Charlot Valdieu, S Buff, E Rosset
The goal of this study was to evaluate the reliability of amniocentesis during late pregnancy to assess lung maturity in puppies using a bubble test as described by Gunston and Davey (South African Medical Journal, 54, 1978, 495). Thirty-five bitches from eight different breeds were followed during late pregnancy before undergoing elective Caesarean (C)-section on days 61-62 after ovulation. Bubble tests were performed the day before the C-section (n = 11 bitches) and before the administration of aglepristone on amniotic fluid samples obtained via amniocentesis and were repeated the day of the surgery on amniotic fluid samples collected via puncture of the amniotic bags before they were opened (n = 35 bitches)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695635/taurine-does-not-improve-the-quality-of-short-term-stored-rabbit-spermatozoa-in-vitro
#19
D Paál, F Strejček, E Tvrdá, J Vašíček, A Baláži, P Chrenek, P Massányi
This study examines the impact of taurine on the viability, morphology and acrosome integrity of rabbit spermatozoa in vitro. Semen samples, obtained from four to five sexually mature and healthy New Zealand White rabbits, were pooled in heterospermic semen sample. This was divided and treated with taurine in a concentration of 0 (control), 1.5, 7, 12.5, 50 mM to a final concentration of 10(8)  spermatozoa/ml. The samples were then incubated at 37°C for 4 hr. A combination of fluorescent probes SYBR-14/propidium iodide/PNA-Alexa Fluor 647 was used to assess spermatozoa viability and acrosome integrity on a flow cytometer...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695606/orally-administered-chrysin-improves-post-thawed-sperm-quality-and-fertility-of-rooster
#20
M Zhandi, M Ansari, P Roknabadi, A Zare Shahneh, M Sharafi
Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
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