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Reproduction in Domestic Animals, Zuchthygiene

D Sankarganesh, R Ramachandran, U Suriyakalaa, V Ramesh Saravanakumar, G Archunan, M A Akbarsha, S Achiraman
Urine samples of female goats in pro-estrus, estrus and post-estrus phases were analyzed for finding estrus-specific volatile compounds using Gas Chromatograph-Mass Spectrometry (GC-MS), and proteins using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization- Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Fourteen urinary volatile were identified covering all three phases among which four compounds, 1-Tetradecanol, n-Pentadecanol, 3-Methylene tridecane, and 2-Ethyl-1-dodecene, were unique to estrus...
January 19, 2019: Reproduction in Domestic Animals, Zuchthygiene
Pouya Dini, Alejandro Esteller-Vico, Kirsten E Scoggin, Peter Daels, Barry A Ball
Archived formalin-fixed, paraffin-embedded (FFPE) samples represent a valuable resource for the determination of gene expression for physio/pathological conditions. In the present study, we validated a protocol for the extraction of RNA from FFPE samples collected from healthy and diseased equine placenta. The quality and quantity of the extracted RNA from the FFPE and matching RNAlater™ -preserved samples and expression levels of common housekeeping genes and reference microRNAs were evaluated. Precision of the expression data was evaluated by comparing relative expression of CYP19A1 and HSD3B1 in FFPE and RNAlater™ samples...
January 19, 2019: Reproduction in Domestic Animals, Zuchthygiene
Priscila Chediek Dall'Acqua, Giovana Barros Nunes, Cíntia Rodrigues da Silva, Patrícia Kubo Fontes, Marcelo Fábio Gouveia Nogueira, Flávia Lombardi Lopes, Márcia Marinho, Gisele Zoccal Mingoti
The epidermal growth factor receptor (EGFR) pathway is directly involved in oocyte meiotic resumption induced by a gonadotropic stimulus. Here, we used an EGFR inhibitor (AG1478) to inhibit spontaneous meiosis resumption in bovine oocytes (EGFR- group) during 8 hours prematuration and assessed the competence of such oocytes for embryonic development, apoptosis and gene expression in comparison to Control group which was not prematured. Data are presented as mean±SEM. Blastocysts rate on day 7 (40.81%, averaged) and hatching rate on day 9 (77...
January 18, 2019: Reproduction in Domestic Animals, Zuchthygiene
P Socha, T Janowski
The objective of study was to derive specific growth curves of ICC and BP for Maine Coon. Twenty-three pregnant queens aged 1 to 6 years, with body weight of 4.5-7.6 kg, were included in the study. In each queen, serial ultrasonographic examinations of pregnancy were performed through the abdomen with the MyLab Gold Vet scanner with a 6.6-7.5 MHz microconvex probe (Turin, Italy). ICC or BP were measured, depending on the stage of pregnancy. Each examination was regarded as a separate observation. In 18 queens, ICC was measured 33 times, and in 21 queens, BP was measured 60 times...
January 18, 2019: Reproduction in Domestic Animals, Zuchthygiene
Mollie K Manier, Glenn Welch, Christiaan Van Nispen, Murray R Bakst, Julie Long
The sperm mobility assay measures the ability of sperm to swim through a dense layer of Accudenz® , and the sperm mobility phenotype has been shown to predict fertility and other sperm performance traits in roosters and turkeys. In this study, we examined turkey sperm morphometry and rates of early embryonic death associated with high and low mobility semen. We also assessed whether the hypo-osmotic stress test, which evaluates the structural integrity of the sperm plasma membrane, may be used as a faster and simpler assay for sperm mobility and viability...
January 16, 2019: Reproduction in Domestic Animals, Zuchthygiene
Ebrahim Ahmadi, Abolfazl Shirazi, Naser Shams-Esfandabadi, Hassan Nazari
Despite the numerous potential applications of oocyte cryopreservation, the poor success rate has limited its practical applications. In livestock, particularly in ovine, the oocytes have low developmental competence following vitrification-warming process. Considering the occurrence of osmotic and oxidative stresses during the vitrification/warming process, the application of antioxidants and osmolytes may improve the developmental competence of vitrified-warmed oocytes. In the present study, we aimed to evaluate the effects of the addition of ascorbic acid (AA) and N-acetyl cysteine (NAC) as antioxidants and glycine as an organic osmolyte either to the vitrification-warming solutions (VWS) or to the IVM medium on the developmental competence of vitrified/warmed ovine GV-stage oocytes...
January 13, 2019: Reproduction in Domestic Animals, Zuchthygiene
M Gaur, G N Purohit
With an objective to evaluate the follicular dynamics and vascularity changes in follicles and corpus luteum the ovaries of cyclic Surti buffaloes (n=9) were examined daily sequentially by transrectal B-mode and color flow mode (CFM) ultrasonography starting from the day of estrus till the onset of next estrus. Higher proportion of buffaloes evidenced one-wave cycle (66.66%) compared to two-wave cycle (33.34%) with none showing a three-wave cycle. The dominant follicle of the first follicular wave was the ovulatory follicle and persisted for 19...
January 6, 2019: Reproduction in Domestic Animals, Zuchthygiene
Denise Silva Okano, Jurandy Mauro Penitente-Filho, Victor Enrique Gomez León, Paula Piccolo Maitan, Camila Oliveira Silveira, Bruna Waddington, Edgar Andrés Díaz-Miranda, Eduardo Paulino da Costa, Simone Eliza Facioni Guimarães, José Domingos Guimarães
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed-time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analyzed in the lab after thawing. Sperm motility and vigor were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2 O2 production were evaluated by flow cytometer...
January 6, 2019: Reproduction in Domestic Animals, Zuchthygiene
Julia Poyato-Bonilla, Gabriel Anaya-Calvo, Antonio Molina, Mercedes Valera, Miguel Moreno-Millán, Jesús Dorado, Sebastián Demyda-Peyrás
Chromosomal abnormalities are a major cause of infertility and reproductive problems in equids. Nowadays, their detection is rising due to the use of new diagnostic tools based on molecular markers instead of karyotyping. Reports of this kind of genetic aberrations in domestic donkeys (Equus asinus) are extremely scarce, despite their importance in human activities. In the present study, we analyzed the implementation of a short-tandem-repeat (STR)-based molecular method initially developed for horses, as a diagnostic tool to detect chromosomal abnormalities in donkeys...
December 29, 2018: Reproduction in Domestic Animals, Zuchthygiene
Tammo Buss, Jörg Aurich, Christine Aurich
In horse breeding, quality assessment of semen before insemination is often requested. Non-laboratory-based techniques for objective analysis of sperm motility are thus of interest. The aim of this study was evaluating a portable device for semen analysis (Ongo sperm test) and its comparison with computer-assisted semen analysis (CASA). Semen was collected from 10 stallions, diluted to 100, 50 and 25 × 106  sperm/ml and analysed for total (TM) and progressive motility (PM). The final sperm concentration influenced total motility analysed by Ongo (p < 0...
December 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Jair Camargo Ferreira, Yatta Linhares Boakari, Noeme Sousa Rocha, Fernanda Saules Ignácio, Guilherme Barbosa da Costa, Cezinande de Meira
The present study characterized the luteal status and the dynamic of the conceptus during the first 20 days of gestation in mares with different ages and degrees of endometrial degeneration. Total area of the corpus luteum (CL), luteal vascularity, CL area with blood signals, progesterone concentrations (P4), embryonic vesicle diameter, number of embryonic location changes, embryonic fixation position and uterine contractility were evaluated. In Experiment 1, mares ≤ 6 years of age (Young group, 5.6±0.2 years, n=7 mares) and mares ≥ 15 years of age (Old group, 17...
December 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
J-L Wang, C Zhang, B Liu, X-M Huang, J-G Dai, J-H Tian, J-M Gao
The effect of berberine (Ber) on in vitro fertilization (IVF) embryo development in pigs and the associated differential expression of microRNAs (miRNAs) in the embryo were investigated. NCSU-23 embryonic culture medium was used for a control group, while NCSU-23 embryonic culture medium added with Ber was used for a Ber group. The embryo development rates in these groups were determined, and the zygotes, 4- and 8-cell embryos, and blastocysts were collected for cDNA microarray analysis. The development rates of 2-, 4-, 8-cell embryos and blastocysts were significantly higher in the Ber group than those in the control group (P < 0...
December 22, 2018: Reproduction in Domestic Animals, Zuchthygiene
María P Turiello, Andrea L Cristofolini, Mariana R Fiorimanti, Tomás Diaz, Lilia R Cavaglieri, Cecilia I Merkis
The aim of this study was to evaluate the effect of feed restriction with or without monensin supplementation, followed by a re-feeding period on cellular apoptosis and proliferation in at term placenta of Anglo-Nubian goats.To evaluate the induction of apoptosis througj the intrinsic pathway proteins Bax and Bcl-2 were determinated. The apoptosis was related with the cell proliferation indices through Ki67 determination. The treatments were applied for 250 days and were (1) ad libitum feeding (control; n=5); (2) restricted feeding at 70% of control (restricted; n=7); and (3) restricted with monensin supplementation (monensin; n=7)...
December 21, 2018: Reproduction in Domestic Animals, Zuchthygiene
Yongjie Wan, Rihong Guo, Mingtian Deng, Zhifei Liu, Jing Pang, Guomin Zhang, Zhibo Wang, Feng Wang
The sheep callipyge (CLPG) phenotype, a well-known muscular hypertrophy syndrome, is caused by an A to G transition in the CLPG1 locus. The mechanisms of CLPG phenotype is very complicated and remain to be further studied. Lacking suitable animal models containing CLPG mutations may partially contributes to these unanswered mechanisms. In this study, we confirmed that the CLPG1 locus, especially the 12-bp CLPG1 motif, is conserved in mammalian animals including rabbit. Then we generated 7 CLPG1-edited rabbits with 100% efficiency using CRIPSR/Cas9 system combined with cytoplasm injection technology...
December 20, 2018: Reproduction in Domestic Animals, Zuchthygiene
A A Igbokwe, O S Iyasere, R A Sobayo, S Iyasere, R I Animashaun, F A Balogun, Z O Aganran, M O Fasola, A D Adedokun, O A Lakehinde, S O Lasisi, M R Suleiman, J D Iyiola, J O Daramola
Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with tris-based extenders containing different levels of TNM (0, 5, 10, 15 and 20mL/100mL extender). The diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter evaluated for sperm functional attributes (percentage motility, acrosome integrity, membrane integrity, abnormality and livability) and oxidative stress (malondialdehyde [MDA] concentration and acrosin activity) parameters...
December 20, 2018: Reproduction in Domestic Animals, Zuchthygiene
S Ben Jemaa, J Ruesche, J Sarry, F Woloszyn, N Lassoued, S Fabre
Mutations in the FecL locus are associated with large variation in ovulation rate and litter size in the French Lacaune sheep breed. It has been shown that the B4GALNT2 gene within the FecL locus is most likely responsible for the high fecundity in the French breed. In this study, we have highlighted the segregation of the FecLL mutation within the B4GALNT2 gene in North African sheep breeds and notably in the highly prolific D'man breed. Genotyping of a sample of 183 Tunisian D'man individuals revealed a high frequency (0...
December 18, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ali Murtaza, Waqas Ahmad, Tariq Sohail, Muhammad Irfan-Ur-Rehman Khan, Imran Mohsin, Muhammad Shahzad, Mujahid Hussain, Muhammad Zahid Tahir, Muhammad Ijaz
This study compares the factors associated with variable interval to estrus and ovulation between early vs. late ovulating goats following PGF2α administration. The extent of ovulation time in Beetal goats (n = 38) was monitored through transrectal ultrasound at every 6 h following a single dose of PGF2α  (experiment 1). Variation in estrus and ovulation times was further explored through the changes in follicular dynamics, endocrine profiles and behavior in another set of goats (n = 13) following single PGF2α  given randomly during the luteal phase (experiment 2)...
December 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
F Pezo, F Romero, F Zambrano, R Sánchez
In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection...
December 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
Patricia Genovese, Emiliano Herrera, Victoria Riaño, Alejandro Bielli
In order to study the effect of undernutrition during gestation on the testicular development in rats and its impact on mitosis, apoptosis and the relative abundance of androgen receptor expression, twenty primiparous 3 months old Sprague-Dawley (Rattus norvegicus) rats, weighing 246 ± 4,0 grams when the experiment began, were mated by the same male. Control group (CG), n=10, fed ad libitum with water and rat chow and restricted group (RG, n=10), fed throughout pregnancy and until birth with 40% of the ad libitum maternal daily feed intake...
November 30, 2018: Reproduction in Domestic Animals, Zuchthygiene
Priscila Del Aguila da Silva, Ricardo Andrés Ramirez Uscategui, Victor José Correia Santos, Augusto Ryonosuke Taira, Renata Sitta Gomes Mariano, Mariana Garcia Kako Rodrigues, Ana Paula Rodrigues Simões, Marjury Cristina Maronezi, Michelle Lopes Avante, Frederico Ozanan Barros Monteiro, Wilter Ricardo Russiano Vicente, Marcus Antonio Rossi Feliciano
The aim of this study was to evaluate the stiffness of fetal lungs, liver, kidneys, and placentomes by acoustic radiation force impulse (ARFI) elastography in ovine and to correlate obtained findings with fetal development. Twenty-four ewes were included and tissue stiffness of fetal lung, liver, kidney, and placentome were measured beginning with 10th gestational week by qualitative-quantitative ARFI elastography. A total of 33 healthy lambs were born. Qualitative elastographic analysis permitted to classify maternal and fetal tissues elasticity in decreasing order as follows: placentome, kidney, liver, and lung...
November 29, 2018: Reproduction in Domestic Animals, Zuchthygiene
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