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Reproduction in Domestic Animals, Zuchthygiene

Maoliang Ran, Bo Weng, Rong Cao, Zhi Li, Fuzhi Peng, Hui Luo, Hu Gao, Bin Chen
Accumulating reports have demonstrated that microRNAs (miRNAs) participate in regulating the complex processes of animal testis development and spermatogenesis; yet, the mechanisms by which miRNAs regulate spermatogenesis are poorly understood. miR-26a was identified as a miRNA that is differentially expressed among different pig testicular tissue developmental stages in our previous study. In this study, p21 activated kinase 2 (PAK2) gene was determined as one target gene of miR-26a by luciferase reporter assay, and miR-26a repressed the PAK2 mRNA abundance in porcine Sertoli cells...
July 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
Elizabete T Gomes, Joana Amélia S Costa, Diogo Manoel F Silva, Wasim Al Shebli, Marina L Azevedo, Pedro L J Monteiro, Robespierre A J Araújo Silva, Antônio S Santos Filho, Maria Madalena P Guerra, Claudio Coutinho Bartolomeu, Aurea Wischral, André M Batista
Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes...
July 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
Raphael da Silva Costa, Caio de Souza Capuzzo, Cristiele da Silva Ribeiro, Rosicleire Verissimo-Silveira, Diógenes Henrique de Siqueira-Silva, José Augusto Senhorini, Alexandre Ninhaus-Silveira
We aimed to vitrify embryos of Prochilodus lineatus in a high-osmolarity cryoprotectant solution, evaluating, after the vitrification-thawing process, their morphological changes. Thus, 240 embryos in the 20-somite phase (20S) were exposed for 20 min to one main internal cryoprotectant solution (1,2-propanediol-PROP), divided into four immersion sequence steps of five minutes each. The first three steps were performed in solutions containing only a main internal cryoprotectant (PROP-2, 3 and 4 M), and the fourth step in a high-osmolarity solution combining internal (PROP + dimethyl sulphoxide-Me2 SO) and external cryoprotectants (sucrose-SUC)...
July 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Weiwei Fu, Ningbo Chen, Shuang Han, Jieping Huang, Fen Li, Ruijie Hao, Hong Chen, Chuzhao Lei, Yun Ma
Adipokines play a crucial role in the regulation of energy homeostasis; however, little is known about genetic alterations in this family that may contribute to economic traits in cattle. Therefore, this study conducts transcript profiles, variations and association studies of three major adipokines, leptin (LEP), tumour necrosis factor (TNF) and angiopoietin-like protein 8 (ANGPTL8), to evaluate their effects on native Chinese cattle. Using quantitative real-time PCR, the study revealed that the bovine LEP was expressed primarily in the back and visceral fat, while TNF was predominantly expressed in spleen and ANGPTL8 was mainly expressed in back fat and liver...
July 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Bahareh Akhtarshenas, Hamed Karami Shabankareh, Hadi Hajarian, Mustafa Numan Bucak, Ali Reza Abdolmohammadi, Mojtaba Dashtizad
The objective of this study was to examine the different concentrations of antipain and trehalose combination on post-thawed quality of ram semen cryopreserved in tris extender. Ejaculates were collected from four rams using the artificial vagina, pooled at 37°C and diluted with (A0  Tre0 : antipain 0 μM and trehalose 0 mM (Control); A10  Tre0 ; A50  Tre0 ; A0  Tre30 ; A0  Tre60 ; A10  Tre60 ; A10  Tre30 ; A50  Tre30 and A50  Tre60 ). Diluted semen samples were gradually cooled down from 37 to 5°C in a cold cabinet; then, they were loaded into 0...
July 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ali Jabarzareh, Ali Sadeghi-Sefidmazgi, GholamReza Ghorbani, Victor Cabrera
In this study, at first, the reproductive consequences of sexed semen use were quantified and then the cost-benefit of sexed semen use on Iranian commercial dairy farms was evaluated. Retrospective data collected during 2006 to 2013 from four large dairy farms in the Isfahan province of Iran were used for this study. These data included of 13,003 heifers records, from which 11.2% used sexed semen from 33 different bulls. All data were analysed using a multivariable logistical regression model, GENMOD procedure from SAS software...
July 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Efigénia S C Camela, Ricardo P Nociti, Victor J C Santos, Beatrice I Macente, Maciej Murawski, Wilter R R Vicente, Pawel M Bartlewski, Maria Emilia F Oliveira
There is a paucity of information on the relationships of testicular morphology, echotextural attributes, and blood flow dynamics with pubertal development of rams raised in a subtropical climate. Forty-five Dorper rams (24 rams aged 8-11 months and 21 rams aged 12-24 months) were examined using a portable ultrasound scanner connected to a 7.5-MHz transducer. Computer-assisted analyses of testicular ultrasonograms utilized commercially available Image ProPlus® analytical software. Spectral Doppler scans of testicular arteries were performed immediately after scrotal (B-mode) ultrasonography to determine peak systolic velocity (PSV), end-diastolic velocity (EDV), resistive index (RI = [PSV-EDV]/PSV), and pulsatility index (PI = [SPV-EDV]/mean velocity) of the blood vessels...
July 10, 2018: Reproduction in Domestic Animals, Zuchthygiene
Shuaishuai Wu, Igor F Canisso, Weigang Yang, Ihteshamu Ul Haq, Qiang Liu, Ying Han, Shenming Zeng
This study aimed to investigate the effects of different concentrations of 1,2-bis-(o-aminophenoxy)-ethane-N,N,N0 N0-tetraacetic acid, tetra-acetoxymethyl ester (BAPTA-AM), an intracellular calcium chelating agent, on stallion semen cooling and freezing-thawing. After collection, semen was extended (1:1 v/v) on a skim milk-based extender, centrifuged and resuspended at 400 million/ml into cooling or freezing extenders containing 0, 5, 25, 50, 100 and 200 μΜ BAPTA-AM. Motility parameters were assessed after cooling in Equitainer at 5°C for 12, 24, 48, 72 and 120 hr and after freezing-thawing...
July 9, 2018: Reproduction in Domestic Animals, Zuchthygiene
Chen Yang, Xiaoxiao Gao, Jing Ye, Jianping Ding, Ya Liu, Hongyu Liu, Xiumei Li, Yunhai Zhang, Jie Zhou, Weiping Huang, Fugui Fang, Yinghui Ling
Epigenetics plays an important role in controlling female puberty. Both DNA methylation and long non-coding RNAs (lncRNA) regulate the initiation of puberty by affecting the expression of genes related to puberty. While recent studies have indicated that DNA methylation of lncRNA represses the expression of lncRNA, its role in regulating puberty remains unclear. To explore the mechanism between DNA methylation and lncRNAs during puberty onset, we performed whole-genome bisulphite sequencing (WGBS) and RNA-sequencing (RNA-seq)...
July 7, 2018: Reproduction in Domestic Animals, Zuchthygiene
Xiusheng Zhu, Lanyu Li, Bangjun Gao, Dandan Zhang, Yanyan Ren, Beibei Zheng, Mengmei Li, Deshun Shi, Ben Huang
To further promote the early development of porcine embryos and capture "naïve" pluripotent state within blastocyst, the experiment explored the effects of lysophosphatidic acid (LPA) on the early development of porcine parthenogenetic embryos and the expression of pluripotency relevant genes. The results showed that the addition of 50 μM LPA significantly improved parthenogenetic embryo cleavage rate (82.7% vs. 74.7%, p < 0.05), blastocyst rate (24.5% vs. 11.3%, p < 0.05) and blastocyst cell count (56 ± 7...
July 5, 2018: Reproduction in Domestic Animals, Zuchthygiene
Orsolya Balogh, Natalie Borruat, Angela Andrea Meier, Sonja Hartnack, Iris M Reichler
Benefits and risks of spaying on the behaviour of female dogs are controversially discussed. Increased aggressiveness and male urinary behaviour were postulated to be the consequence of masculinization after spaying in some female dogs. To investigate if spaying or its timing relative to the onset of puberty may have a masculinization effect, urinary behaviour, that is, frequency of urination, urinary posture and ground scratching after urination were recorded in 58 female Labrador Retrievers during 15 min of a daily walk with their owners...
July 5, 2018: Reproduction in Domestic Animals, Zuchthygiene
Sergei Amstislavsky, Eugeny Brusentsev, Elena Kizilova, Valentina Mokrousova, Valeria Kozhevnikova, Tatyana Abramova, Irina Rozhkova, Sergey Naidenko
The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38...
July 2, 2018: Reproduction in Domestic Animals, Zuchthygiene
Yinhai Jia, Xiurong Yang, Chengfu Zhang, Shangzhi Yang, Ming Li, Wenwen Xu, Qiumei Ji, Hesheng Jiang
The yak is one of the most important and economically useful animals for highlanders. The decline in the yak population requires effective measures for the conservation and multiplication of elite germplasm. A standardized protocol will simplify the freezing and warming of yak embryos in straw and facilitate embryo transfer. In this work, we investigated a one-step protocol that uses a stable basal medium, which comprised a warming medium (1.08 M sucrose) and a freezing medium (EFS40). We also assessed the effects of the new transfer method on embryo survival...
July 2, 2018: Reproduction in Domestic Animals, Zuchthygiene
Daniel Veraguas, Sandra R Cuevas, Paula F Gallegos, Darling Saez-Ruiz, Fidel O Castro, Lleretny Rodriguez-Alvarez
The objective of this study was to evaluate the eCG stimulation on domestic cat oocyte competence during the non-breeding season. Four experimental groups were made: (a) untreated cycling cats (Breeding season group; BS), (b) untreated anestrous cats (Non-breeding group; NB), (c) anestrous cats treated with 200 IU of eCG (eCG group) and (d) anestrous cats treated with 200 IU of eCG and 100 IU of hCG four days later (hCG group). In the BS, NB and eCG groups, grade I and II immature cumulus-oocyte complexes (COCs) were subjected to in vitro maturation or used for the gene expression analysis of FSHR, LHCGR, EGFR, EGR1, ESR2, PTGS2, GDF9, BMP15 and GATM...
June 29, 2018: Reproduction in Domestic Animals, Zuchthygiene
Krzysztof Jagiełło, Stefan Dobosz, Tomasz Zalewski, Marcin Polonis, Konrad Ocalewicz
Poor quality eggs produced by the fully homozygous doubled haploids (DHs) may impair generation of clonal lines in fish species. In the present research, gynogenetic development of rainbow trout (Oncorhynchus mykiss) was induced in eggs originated from the DH females. Eggs were activated with the UV-irradiated grayling (Thymallus thymallus) spermatozoa and subjected to the high hydrostatic pressure (HHP) shock to provide diploid clonal individuals. Only two of four DH females produced eggs that were successfully activated by the irradiated spermatozoa and subsequently developed into the gynogenetic embryos...
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Firdous A Khan, Cathy J Gartley, Afroza Khanam
This review article is aimed at providing an update on canine cryptorchidism based on the research studies published in this area over the last 26 years. Although a majority of these research studies were directed at investigating the genetic and molecular basis of cryptorchidism, the mechanisms underlying this problem in dogs are still not clear. Future studies using advanced genomic and molecular approaches and including a larger number of dogs of multiple breeds are required to further our understanding of canine cryptorchidism...
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Rajani K Paul, Krishnappa Balaganur, Davendra Kumar, Syed M K Naqvi
Seminal plasma (SP) is known to induce motility and capacitation in spermatozoa curtailing their lifespan when preserved. Hence, this study was conducted to examine the effects of removal of SP from sperm surface prior to liquid preservation either by high dilution (1/15) or by washing and the poststorage treatment with SP (15% and 25%, v/v) on the quality attributes of liquid-preserved ram semen. Over the period of storage, the rapid motility (66.0% and 71.1% vs. 58.3%), straightness (87.1% and 82.1% vs. 79...
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Hao Li, Yi-Lin Bian, Nicola Schreurs, Xiao-Gang Zhang, Sayed Haidar Abbas Raza, Qian Fang, Li-Qiang Wang, Jian-Hong Hu
The cryopreservation of testicular tissue is a potential method for preserving male fertility. However, the effect of cryopreservation on bovine calf testicular tissue is scarce. This study investigated the effect of different cryoprotectants on bovine calf testicular tissue at the molecular level. Testicular tissue from ten immature bovine calves (6 months) was collected after slaughter and cryopreserved in an extender containing different concentrations of the following five cryopreservation solutions (CP): bovine serum albumin (BSA) with 5% dimethyl sulfoxide (DMSO), trehalose with 5% DMSO, DMSO and glycerol and ethylene glycol (EG)...
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ana Martins-Bessa
No abstract text is available yet for this article.
June 28, 2018: Reproduction in Domestic Animals, Zuchthygiene
Longfei Xiao, Junjie Hu, Xingxu Zhao, Liangli Song, Yong Zhang, Weitao Dong, Quanwei Zhang, Youji Ma, Fadi Li
Melatonin is an important factor involved in regulating reproduction; it is synthesized enzymatically by the sequential action of melatonin-synthesizing enzymes, arylalkylamine N-acetyltransferase (AANAT) and hydroxyindole-O-methyltransferase (HIOMT), and exerts its biological functions mainly through receptor-mediated action. To evaluate the expression of melatonin, two melatonin-synthesizing enzymes (HIOMT and AANAT), and membrane receptors (MT1 and MT2) in oestrous corpus luteum (CL) and CL verum of sheep (Ovis aries), we performed ELISA, qRT-PCR, western blotting and immunohistochemistry...
June 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
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