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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28332740/ultrasound-during-mid-gestation-agreement-with-physical-foetal-and-placental-measurements-and-use-in-predicting-gestational-age-in-sheep
#1
A K Jones, R E Gately, K K McFadden, M L Hoffman, S M Pillai, S A Zinn, K E Govoni, S A Reed
To determine the effects of poor maternal nutrition and litter size on foetal growth during mid-gestation, pregnant ewes (n = 82) were fed 100%, 60% or 140% of NRC TDN beginning at day 30.2 ± 0.2 of gestation. Transabdominal ultrasound was performed weekly between day 46.0 ± 0.4 and 86.0 ± 0.7 to monitor foetal heart width (HW), umbilical diameter (UMB), rib width (RW) and placentome outer (OD) and inner diameter (ID). Data were analysed with repeated-measures using the mixed procedure for effects of maternal diet, litter size and gestation, and equations predictive of gestational age were generated using the regression procedure...
March 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28332739/gdf9-affects-the-development-and-tight-junction-functions-of-immature-bovine-sertoli-cells
#2
K Tang, L Wang, Y Jin, W Yang, L Yang
Transforming growth factor-β (TGFβ) superfamily are critical regulators of germ cell development that act as extracellular ligands of the signal transduction pathways regulating proliferation, apoptosis and other aspects of cell behaviour. As a member of the TGF-β superfamily, growth differentiation factor 9 (GDF9) plays a critical role in ovarian follicular development and the ovulation rate in females; however, its role in the testis has not been well elucidated. The aim of this study was to investigate the expression of GDF9 and its receptor genes BMPRII and ALK5 in prepuberal bovine Sertoli cells (SCs)...
March 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28332278/a-comparative-study-of-the-effects-of-intramuscular-administration-of-gonadorelin-mating-and-intrauterine-infusion-of-either-raw-seminal-plasma-or-seminal-plasma-purified-%C3%AE-ngf-on-luteal-development-in-llamas
#3
M Silva, F Urra, C Ulloa-Leal, M H Ratto
The aim of this study was to compare the effect of the intramuscular administration of 50 μg of gonadorelin acetate versus natural mating, intrauterine infusion (i.u.) of a physiological relevant dose of either raw llama seminal plasma (SP) or purified beta-nerve growth factor from seminal origin (spβ-NGF) on ovulation rate and corpus luteum (CL) development and function in llamas. Females with a follicle (≥8 mm) were assigned to groups: (i) i.m. administration of 50 μg of gonadorelin acetate (GnRH; positive control; n = 4); (ii) single mating (mating; n = 6); (iii) i...
March 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28332242/bilateral-oblique-facial-clefts-rudimentary-eyes-and-hydrocephalus-in-an-aborted-equine-foetus
#4
J S Agerholm, H G Pedersen, F J McEvoy, S Heegaard
Knowledge of congenital malformations and their causes in horses is generally sparse. Such conditions require more scientific attention to improve their diagnostics and inform prevention strategies. Here, a unique syndrome of bilateral oblique facial clefts (meloschisis), rudimentary eyes and hydrocephalus is reported in an equine foetus spontaneously aborted at gestation day 224. The cause of abortion was considered to be intrauterine death caused by umbilical cord torsions and subsequent compromised blood flow, but the aetiology of the malformation could not be determined...
March 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28332235/toll-like-receptor-and-related-cytokine-mrna-expression-in-bovine-corpora-lutea-during-the-oestrous-cycle-and-pregnancy
#5
J E Gadsby, A M Tyson Nipper, H A Faircloth, M D'Annibale-Tolhurst, J Chang, P W Farin, I M Sheldon, D H Poole
Improving our understanding of the mechanisms controlling the corpus luteum (CL) and its role in regulating the reproductive cycle should lead to improvements in the sustainability of today's global animal industry. The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture steroidogenic, endothelial and immune cells, and it is becoming clear that immune mechanisms play a key role in CL regulation especially in luteolysis. Toll-like receptors (TLR) mediate innate immune mechanisms via the production of pro-inflammatory cytokines, especially within various tissues, although the role of TLR within CL remains unknown...
March 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326632/reproductive-performance-of-postpartum-ewes-treated-with-insulin-or-progesterone-hormones-in-association-with-ram-effect
#6
J C Ferreira-Silva, Srl Basto, F Tenório Filho, M T Moura, M L Silva Filho, Mal Oliveira
The reproductive performance of postpartum Santa Inês (SI) and Morada Nova (MN) ewes treated with insulin or progesterone hormones in association with ram effect was evaluated. Ewes from SI (n = 69) and MN (n = 69) breeds were randomly allocated to three groups of each breed (T1-ram effect only; T2-ram effect + insulin; T3-ram effect + progesterone). Progesterone concentrations (ηg/ml; mean ± SD) before and after introduction of rams (n = 6) were 0.51 ± 0.22 and 3.78 ± 0.68 (T1), 0.65 ± 0...
March 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326634/influence-of-partial-or-total-replacement-of-glycerol-by-alternative-cryoprotectants-in-ghent-freezing-extender-on-post-thaw-sperm-quality-in-stallions
#7
R A De Oliveira, S Budik, C Aurich
Although glycerol is the cryoprotectant most commonly used in stallions, it has also a considerable toxicity for equine sperm. It was the aim of this study to analyse the quality of frozen-thawed stallion semen after complete or partial replacement of glycerol in the freezing extender by alternative cryoprotectants. We hypothesized that partial or total replacement of glycerol by cryoprotectants occurring in cold-resistant frog, insect or plant species results in similar or better semen quality after freezing-thawing...
March 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326621/effects-of-single-layer-centrifugation-slc-on-bull-spermatozoa-prior-to-freezing-on-post-thaw-semen-characteristics
#8
T Nongbua, A Johannisson, A Edman, J M Morrell
Single layer centrifugation (SLC) has been shown to select the most robust spermatozoa from the ejaculate in several species. Here the effects of SLC prior to freezing on various parameters of frozen-thawed bovine sperm quality are reported. Semen from 8 bulls was layered on top of a species-specific colloid, Bovicoll. After centrifugation for 20 min at 300 g, the resulting sperm pellet was resuspended in OPTIXcell(®) (IMV Technologies, l'Aigle, France); the SLC-selected sperm samples and uncentrifuged controls were frozen...
March 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326620/effect-of-selenium-and-vitamin-e-addition-to-the-extender-on-liquid-stored-capercaillie-tetrao-urogallus-semen-quality
#9
A M Kowalczyk, J Klećkowska-Nawrot, E T Łukaszewicz
Captive breeding has become an important tool in species conservations programmes, maintaining genetic diversity and restoring wild, endangered populations. In order to improve the reproductive efficiency of captive kept capercaillie, the purpose of the study was to determine the effect of selenium and vitamin E addition to semen extender on sperm characteristic during short-term storage. Ejaculates collected individually from four capercaillie were divided into two parts, diluted threefold with basic EK extender and EK enriched with 1 mg/ml of organic selenium and 8 mg/ml of vitamin E (EK+Se+E) and stored 24 hr at temp...
March 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28326614/recombinant-expression-of-porcine-spermadhesin-awn-and-its-phospholipid-interaction-indication-for-a-novel-lipid-binding-property
#10
F Schröter, K Müller, P Müller, E Krause, B C Braun
AWN is a porcine (Sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. To acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in E. coli and a three-step purification protocol based on different chromatographies. The test for AWN-phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. As phosphatidic acid is surmised to play a role in cation-induced membrane destabilization and fusion events, we propose a membrane protective function of the presented binding affinity...
March 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28294422/-post-mortem-examination-of-the-reproductive-organs-of-female-wild-boars-sus-scrofa-in-sweden
#11
Anna Malmsten, Gunnar Jansson, Anne-Marie Dalin
In recent decades, wild boars (Sus scrofa) have increased in numbers and distribution in Europe. Compared to other wild ungulates of similar body size, wild boars have a high reproductive capacity. To increase the knowledge of wild boar reproduction, the objective of this study was to investigate characteristics of reproductive organs, and to provide information on the occurrence of abnormalities in reproductive organs from free-ranging female wild boars. Between December 2011 and December 2015, reproductive organs from female wild boars (>30 kg body weight), were collected during hunting in four Swedish counties at estates where supplementary feeding was applied...
March 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295702/effect-of-steroid-hormones-on-the-innate-immune-response-induced-by-staphylococcus-aureus-in-the-goat-mammary-gland
#12
K Kuwahara, Y Yoshimura, N Isobe
The objective of this study was to compare the dynamics of innate immune components after intramammary infusion of Staphylococcus aureus (SA) under conditions of high oestrogen and high progesterone in goats. In one group ("E-group"), controlled internal drug release (CIDR) devices were inserted intravaginally from days -11 to -4. Prostaglandin F2α was administered immediately after removal of the CIDR device at day -3, and then oestradiol benzoate (E) was injected intramuscularly once a day from days -2 to 3...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295662/chilled-and-post-thaw-storage-of-sperm-in-different-goldfish-types
#13
G Bernáth, I Ittzés, Z Szabó, Á Horváth, S Krejszeff, J Lujić, L Várkonyi, B Urbányi, Z Bokor
The effective storage time of sperm after stripping (for 48 hr in 6-hr intervals) and after thawing (for 6 hr in 2-hr intervals) in Black moor, Oranda and Calico goldfish types was investigated. Variations in sperm density were also measured in all lines. The efficiency of a sperm cryopreservation method formerly developed for common carp was recorded in all three goldfish lines. Motility parameters ((pMOT, %), curvilinear velocity (VCL, μm/s) and straightness (STR, %)) of Black moor sperm did not decrease significantly during 48 hr of storage...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295644/effect-of-vitrification-on-the-mrna-transcriptome-of-bovine-oocytes
#14
N Wang, C-Y Li, H-B Zhu, H-S Hao, H-Y Wang, C-L Yan, S-J Zhao, W-H Du, D Wang, Y Liu, Y-W Pang, X-M Zhao
Vitrification has been shown to decrease the developmental capacity of mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of vitrified oocytes. However, the effect of vitrification on transcriptional machinery of oocytes examined by RNA sequencing (RNA-seq) has yet to be defined. In the present study, the mRNA transcriptomes of fresh and vitrified bovine oocytes were analysed by Smart-seq2 with the differently expressed genes determined by DEseq2 (an adjusted p-value of .05 and a minimum fold change of 2)...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28294426/polymorphism-in-5-untranslated-region-of-heat-shock-protein-70-gene-as-marker-of-post-partum-anoestrus-in-murrah-buffaloes
#15
R Kumar, M Ghosh, N Kumar, A K Balhara, M Gupta, R K Sharma, I Singh
The enormous production potential of buffaloes has never been accomplished due to various reproductive insufficiencies. Among them, post-partum anoestrus, a multifactorial disorder, is predominant but any genetic association is yet to be established. This study focused to identify novel polymorphisms in heat-shock protein 70 (HSP70) gene and its possible association with post-partum anoestrus in Murrah buffaloes. A 579-bp fragment from 5' untranslated region of HSP70 gene was amplified by polymerase chain reaction from blood genomic DNA of 614 animals maintained under similar management conditions...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295721/endometrial-biopsy-in-bos-indicus-beef-heifers
#16
O Ramirez-Garzon, N Satake, R E Lyons, J Hill, M K Holland, M McGowan
An endometrial biopsy allows for a comprehensive assessment of the uterine environment of a breeding female. Although routine in mares, devices used for endometrial biopsies are impracticable in heifers due to the size and structure of the cervix. This report describes the use of a human bronchoscopy biopsy device (Karl Storz(®) 10366L) for collection of endometrial biopsies in Bos indicus beef heifers. The Storz(®) device is smaller and thinner and enabled the collection of an endometrial biopsy in 86% of heifers (n = 44/51)...
March 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295710/effects-of-supplementation-of-medium-with-different-antioxidants-during-in-vitro-maturation-of-bovine-oocytes-on-subsequent-embryo-production
#17
T C Sovernigo, P R Adona, P S Monzani, S Guemra, Fda Barros, F G Lopes, Clv Leal
The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse-derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295704/comparison-of-the-effects-of-ham-sf10-and-%C3%AE-mem-in-combination-with-fbs-or-bsa-in-vitrification-warming-solution-on-quality-and-viability-of-sheep-ovarian-follicles
#18
F Mohammadzadeh, L Safdarian, F Amidi, A Mohammadzadeh, K Mortezaee, S Mehdinejhadiani, A Sobhani, S Ghasemi, F Sargolzaei Aval
The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295703/peri-conceptional-under-nutrition-alters-the-expression-of-trim28-and-zfp57-in-the-endometrium-and-embryos-during-peri-implantation-period-in-domestic-pigs
#19
K Zglejc, A Franczak
DNA methylation is maintained by the main elements of methylation complex-tripartite motif containing 28 (TRIM28) and zinc finger protein 57 (ZFP57). Previously, it was found that the activity of TRIM28 and ZFP57 determines the process of DNA methylation and preserves over-expression of genes. We hypothesized that restricted diet applied during peri-conceptional period may induce changes in the expression of methylation complex in porcine endometrium and embryos during the peri-implantation period. The aim of this study was to detect and determine the expression of TRIM28 and ZFP57 in the endometrium and embryos harvested from gilts during the peri-implantation period (days 15-16 of pregnancy) fed restricted (n = 5) or normal (n = 5) diet during peri-conceptional period...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28294447/development-of-an-in-vitro-oviduct-epithelial-explants-model-for-studying-sperm-oviduct-binding-in-the-buffalo
#20
K K Saraf, A Kumaresan, S Nayak, S Chhillar, L Sreela, S Kumar, U K Tripathi, T K Datta, T K Mohanty
In this study, we developed an in vitro model for studying sperm-oviduct binding in the buffalo. Oviduct explants were prepared by overnight culture of epithelial cells in TCM-199 medium under 5% CO2 at 38.5 °C. Cryopreserved spermatozoa from buffalo bulls (n = 4) were incubated with the oviduct explants, and the sperm-oviduct explants complex was stained with JC-1. The effect of sperm concentration (2, 3 and 4 million), size of the oviduct explants (<0.2, 0.2-0.3, 0.3-0.4 and >0.4 mm(2) ) and time of incubation (1 hr and 4 hr) on binding index (BI-number of sperm bound to unit area of explants) was studied...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
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