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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28695674/foetal-pulmonary-maturity-in-dogs-estimated-from-bubble-tests-in-amniotic-fluid-obtained-via-amniocentesis
#1
T Bonte, A Del Carro, J Paquette, A Charlot Valdieu, S Buff, E Rosset
The goal of this study was to evaluate the reliability of amniocentesis during late pregnancy to assess lung maturity in puppies using a bubble test as described by Gunston and Davey (South African Medical Journal, 54, 1978, 495). Thirty-five bitches from eight different breeds were followed during late pregnancy before undergoing elective Caesarean (C)-section on days 61-62 after ovulation. Bubble tests were performed the day before the C-section (n = 11 bitches) and before the administration of aglepristone on amniotic fluid samples obtained via amniocentesis and were repeated the day of the surgery on amniotic fluid samples collected via puncture of the amniotic bags before they were opened (n = 35 bitches)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695635/taurine-does-not-improve-the-quality-of-short-term-stored-rabbit-spermatozoa-in-vitro
#2
D Paál, F Strejček, E Tvrdá, J Vašíček, A Baláži, P Chrenek, P Massányi
This study examines the impact of taurine on the viability, morphology and acrosome integrity of rabbit spermatozoa in vitro. Semen samples, obtained from four to five sexually mature and healthy New Zealand White rabbits, were pooled in heterospermic semen sample. This was divided and treated with taurine in a concentration of 0 (control), 1.5, 7, 12.5, 50 mM to a final concentration of 10(8)  spermatozoa/ml. The samples were then incubated at 37°C for 4 hr. A combination of fluorescent probes SYBR-14/propidium iodide/PNA-Alexa Fluor 647 was used to assess spermatozoa viability and acrosome integrity on a flow cytometer...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28695606/orally-administered-chrysin-improves-post-thawed-sperm-quality-and-fertility-of-rooster
#3
M Zhandi, M Ansari, P Roknabadi, A Zare Shahneh, M Sharafi
Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples)...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691383/different-origins-of-two-corpora-lutea-recovered-from-a-pregnant-african-elephant-loxodonta-africana
#4
Y Yamamoto, K Nagaoka, Y Kamite, G Watanabe, T Allen, F Stansfield, K Taya
Elephant ovaries contain multiple corpora lutea (CLs) throughout pregnancy. Two CLs (P-1 and P-2) collected from a pregnant African elephant were used to investigate their origin and physiological state in this study. The mRNA expressions of prolactin receptor, CYP11A and inhibin betaB subunit were higher in P-2 than in P-1, while LHCGR and inhibin betaA subunit mRNA were higher in P-1 than in P-2. Protein expression of cleaved caspase-3 was detected in P-1 but not in P-2. These results suggest different origins for the two CLs in this one pregnant elephant, and we also demonstrated the production of bioactive prolactin by the elephant placenta...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691353/reproductive-performance-of-immobilized-cryopreserved-bovine-semen-used-for-timed-artificial-insemination
#5
A H Alm-Kristiansen, G Dalen, G Klinkenberg, L Bekk, L T Thorkildsen, K E Waterhouse, E Kommisrud
The SpermVital(®) technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after AI. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization technology in combination with cryopreservation. Sperm cell survival (acrosome and plasma membrane integrity) was studied in vitro for 48 hr at physiological temperature. One dose of SpermVital(®) (SV) semen was compared with single doses of Biladyl(®) (B) processed semen as well as double doses of B (B double)...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691350/serum-testosterone-sperm-quality-cytological-physicochemical-and-biochemical-characteristics-of-the-prostatic-fraction-of-dogs-with-prostatomegaly
#6
A Aquino-Cortez, B Q Pinheiro, Hvr Silva, Dbc Lima, Tfp Silva, M B Souza, D A Viana, Faf Xavier Júnior, Jsam Evangelista, F Z Brandão, Ldm Silva
Prostatomegaly is a common finding in older non-neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm-rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691349/ovsynch-plus-protocol-improves-ovarian-response-in-anovular-murrah-buffaloes-in-low-breeding-season
#7
R K Sharma, S K Phulia, A Jerome, I Singh
The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (n = 55) were divided into two groups: Ovsynch group (n = 26): GnRH (10 μg, GnRH1) on Day 0, PGF2 α (25 mg) on Day 7, GnRH (10 μg, GnRH2) on Day 9; Ovsynch Plus group (n = 29): 500 IU equine chorionic gonadotropin (eCG) 72 hr (day -3) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24 hr after GnRH2 injection in bot groups...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691325/cellular-and-extracellular-vesicular-origins-of-mirnas-within-the-bovine-ovarian-follicle
#8
G M Andrade, F V Meirelles, F Perecin, J C da Silveira
The ovarian follicle components must provide an ideal environment to ensure the success of reproductive processes, and communication between follicular cells is crucial to support proper oocyte growth. Recently, it has been demonstrated that the presence of extracellular vesicles (EVs) carrying microRNAs (miRNAs) in follicular fluid represents an important autocrine and paracrine communication mechanism inside the ovarian follicle. In this study, we tested the hypothesis that the miRNA content of EVs isolated from ovarian follicular (granulosa and cumulus-oocyte complexes) cell-conditioned culture media is dependent upon cell type...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691312/paraoxonase-1-pon1-activity-in-serum-follicular-fluid-and-seminal-plasma-of-sheep
#9
J Pradieé, F T de Campos, Jaa Rincon, L Collares, K Goularte, Pas Silveira, Lmc Pegoraro, A Schneider
This work aimed to describe the activity of paraoxonase 1 (PON1) in serum, follicular fluid and seminal plasma of sheep. Average serum PON1 activity was 286.8 ± 96.2 U/ml in females and 237.6 ± 18.9 U/ml in males. There was a positive correlation between PON1 activity in serum and follicular fluid in females, being twice higher in serum than in follicular fluid (148.8 ± 15.7 U/ml). PON1 activity in males' serum was 10-fold higher than in seminal plasma (21.18 ± 14.2 U/ml), and there was no correlation between PON1 activity in both compartments...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691287/effects-of-iodine-methionine-on-boar-sperm-quality-during-liquid-storage-at-17%C3%A2-c
#10
Q Fang, J Wang, Y Y Hao, H Li, J X Hu, G S Yang, J H Hu
Microbial environment is one of the important factors that affect the quality of preserved semen. Iodine methionine (IM), participating in the production and activation of metabolic enzymes, is a new type of amino acid chelate. To date, there has been no report to evaluate the effects of IM on boar semen preservation at 17°C. This study was designed to investigate the effects of IM on boar sperm quality and reproductive performance during liquid storage at 17°C and its antibacterial effect. Semen samples collected from six Yorkshire boars were diluted with basic liquid containing different concentrations of IM (0, 20, 40, 80, 160 and 320 μM)...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28691249/seasonal-or-pathological-findings-morphofunctional-characteristics-of-the-equine-endometrium-during-the-autumn-and-spring-transition
#11
R Killisch, D Böttcher, T Theuß, H Edzards, G Martinsson, A Einspanier, J Gottschalk, H-A Schoon
The deep anoestrous phase in winter is part of the anovulatory season in mares and is bordered by the autumn and spring transitional periods (ATP/STP). To define an annual time span for effective prognostic biopsy sampling, the aim of this study was to provide a morphofunctional characterization of the endometrium during ATP and STP. To outline both transitional periods, endometrial specimens were taken in September, October and November (n = 76) as well as February, March and April (n = 184) with the requirement of a detailed clinical documentation...
July 9, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28660693/relationship-of-temperament-with-oestrous-behaviour-resumption-of-ovarian-cyclicity-and-milk-yield-in-post-partum-murrah-buffaloes
#12
K K Choudhary, A Bharadwaj, R K Sharma, A Jerome, S Khanna
This study was designed to investigate the relationship of temperament with oestrus, resumption of ovarian cyclicity and milk yield in post-partum buffaloes. For this study, 102 post-partum Murrah buffaloes were observed for temperament in open paddock and during milking. Based on the temperament score (1-5 score), they were classified into docile, slightly restless, restless, aggressive and nervous. Oestrous behaviour was monitored twice daily in all the buffaloes along with milk yield (weekly), body weight (BW) and body condition score (BCS) at day 0, 30, 60 and 90 post-partum...
June 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28660684/antioxidative-effect-of-bha-in-soya-bean-lecithin-based-extender-containing-glycerol-or-dmso-on-freezing-capacity-of-goat-semen
#13
M Rahmatzadeh, H Kohram, A Zare Shahneh, A Seifi-Jamadi, E Ahmad
The aim of this study was to evaluate the effects of butylated hydroxyanisole (0 or 4 mM) along with different concentrations (5 or 7%) of glycerol (G) and dimethyl sulphoxide (DMSO) as cryoprotectant (CPAs) on freezability of goat semen. Semen was collected from four bucks (3-4 years) twice a week for five weeks. The pooled ejaculates were diluted with extender containing two different concentrations of G or DMSO in combination with BHA. Afterwards, the diluted samples were loaded into 0.25 ml straws and frozen using a standard protocol...
June 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28660662/chlorogenic-acid-supplementation-during-in-vitro-maturation-improves-maturation-fertilization-and-developmental-competence-of-porcine-oocytes
#14
T-V Nguyen, F Tanihara, Ltk Do, Y Sato, M Taniguchi, M Takagi, T Van Nguyen, T Otoi
Chlorogenic acid (CGA) is a quinic acid conjugate of caffeic acid, and a phytochemical found in many fruits and beverages that acts as an antioxidant. The present study investigated the effects of CGA supplementation during in vitro maturation (IVM), on in vitro development of porcine oocytes, to improve the porcine in vitro production (IVP) system. Oocytes were matured either without (control) or with CGA (10, 50, 100 and 200 μM). Subsequently, the matured oocytes were fertilized and cultured in vitro for 7 day...
June 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28660630/cryopreservation-of-nili-ravi-buffalo-bubalus-bubalis-semen-in-andromed-%C3%A2-extender-in-vitro-and-in-vivo-evaluation
#15
M S Ansari, B A Rakha, S Akhter
The study was designed to evaluate AndroMed(®) for the freezability and fertility of Nili-Ravi buffalo semen. Semen was collected from four adult Nili-Ravi buffalo (Bubalus bubalis) bulls for 3 weeks (replicate). Semen ejaculates from each buffalo bull were divided into three aliquots. One aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, DNA integrity and normal apical ridge. Remaining two aliquots were diluted (37°C; 50 × 10(6) spermatozoa/ml) in tris-citric egg yolk or AndroMed(®) extender and cryopreserved in 0...
June 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28627068/feed-restriction-regime-in-a-rabbit-line-selected-for-growth-rate-alters-oocyte-maturation-manifested-by-alteration-in-msy2-gene-expression
#16
C Naturil-Alfonso, D S Peñaranda, J S Vicente, F Marco-Jiménez
Young rabbit females selected for growth rate may have nutritional needs, which may not be met with the common practice of feed restriction during rearing in commercial rabbit production. The aim of this study was to analyse whether two different feeding programmes: ad libitum or restricted (130 g/day) feeding, applied in young rabbit females for 1 month at the end of rearing, could modulate the origin of ovulation process and the quality of the oocytes. At 16 weeks of age, 34 females were randomly assigned to restricted or ad libitum feeding, maintaining these conditions for a month...
June 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28608426/monitoring-of-libido-and-semen-quality-parameters-in-melatonin-treated-french-alpine-bucks-during-the-non-breeding-season
#17
S Vince, H Valpotić, V Berta, S Milinković-Tur, M Samardžija, J Grizelj, B Špoljarić, D Đuričić, I Nazansky, I Žura Žaja
The aim of this study was to investigate the effects of exogenous melatonin on libido and semen quality parameters in bucks during the non-breeding season. Twelve bucks of the French alpine breed from 1.5 to 4 years of age were assigned into melatonin (MG) and control (CG) groups, with 6 bucks in each group. The experimental period was 3 months (March-May), divided into six periods of 15 days each. The bucks in the MG group received four melatonin implants at the end of March. Two semen samples were taken from the bucks by artificial vagina once per week and their libido estimated...
June 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28585345/genetic-screening-of-fecb-fecx-g-and-fecx-i-mutations-and-their-linkage-with-litter-size-in-barki-and-rahmani-sheep-breeds
#18
A S El-Seedy, N M Hashem, K M El-Azrak, Anm Nour El-Din, T A Ramadan, T A Taha, M H Salem
Characterization of fecundity genes offers the opportunity to improve production efficiency, and the consequent increase in litter size in livestock industry, through utilizing them in breeding programs. The main objective of this study was to detect the BMPR-IB, BMP15 and GDF9 gene mutations and to investigate whether these mutations are associated with litter size in Egyptian sheep breeds. To achieve this goal, 73 adult ewes representing Barki (n = 33) and Rahmani (n = 40) breeds were used. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) screening approach was used to detect the presence of FecB, FecX(G) and FecX(I) mutations in the two selected breeds...
June 5, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28581128/effect-of-dietary-vitamin-e-and-selenium-supplementation-on-semen-quality-in-cairn-terriers-with-normospermia
#19
K T Kirchhoff, K Failing, S Goericke-Pesch
Among others, selenium (Se) and vitamin E (VitE) have been provided to dogs to improve semen quality. However, scientific evidence documenting an effect in dogs is lacking. The aim of this study was to investigate the effect of supplementation of these antioxidants on various ejaculate parameters in a randomized, double-blinded trial using Cairn Terrier males exhibiting normal seminal quality parameters. Three dogs each were fed a standardized diet and supplemented with 0.1 mg Se, 100 mg VitE or 0.1 mg Se + 100 mg VitE/dog for 3 months...
June 5, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28580754/regional-distribution-and-integrity-of-equine-ovarian-pre-antral-follicles
#20
S M Gonzalez, C B da Silva, A G Lindquist, I Bufalo, F Morotti, L A Lisboa, M Seneda
The goal of this study was to determine the distribution of pre-antral follicles in the ovarian parenchyma of mares. For Experiment 1, each ovary was cut longitudinally at the greater curvature, performing two hemiovaries. After that, six fragments from each hemiovary were obtained, resulting in 12 fragments, which were divided into the innermost region of the parenchyma, the middle region and the outermost region. All the three obtained sections were cut transversally to obtain two fragments from each one...
June 4, 2017: Reproduction in Domestic Animals, Zuchthygiene
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