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Reproduction in Domestic Animals, Zuchthygiene

Fernando Sánchez-Dávila, Rodolfo Ungerfeld, Alejandro Sergio Del Bosque-González, Hugo Bernal-Barragán
The aim of this study was to determine the influence of the season of birth on the body weight, testicular size, testosterone concentrations, semen output and sexual behavior in Saint Croix male lambs. Thirty-three lambs were born in spring (n = 10), summer (n = 12), or autumn (n = 11). Body weight and the scrotal circumference were weekly recorded; semen was also weekly collected by electroejaculation, and sexual behavior toward estrous ewes was weekly evaluated. Data were recorded from 15 to 33 weeks of age...
November 9, 2018: Reproduction in Domestic Animals, Zuchthygiene
P Garcia, K Aspee, G Ramirez, P Dettleff, J Palomino, O A Peralta, V H Parraguez, M De Los Reyes
Growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus-oocytes complexes (COCs) were cultured in six groups for 72 h in a supplemented TCM199-Hepes medium as 1) Control group; 2) GDF-9 antibody (Ab); 3) BMP-15 Ab; 4) recombinant human (rh) GDF-9; 5) rh BMP-15 or 6) rh BMP-15 and GDF-9. Data were evaluated by ANOVA...
November 2, 2018: Reproduction in Domestic Animals, Zuchthygiene
Hirenallur Maheshwarappa Yathish, Subodh Kumar, Rajni Chaudhary, Chinmoy Mishra, Sivakumar Ayyar, Amit Kumar, Anuj Chauhan, Subrata Kumar Ghosh, Manjit Panigrahi, Abhijit Mitra
Transition proteins (TNPs) are essential in chromatin condensation during spermiogenesis and hence they are the candidate genes for identifying sperm motility markers. Coding and in-silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR-SSCP analysis revealed two band patterns in fragment III of TNP1 and fragment II of TNP2 gene. Sequence analysis revealed a deletion of 'G' nucleotide in 3'UTR region of TNP1 and C>T SNP in intronic region of TNP2 gene...
October 29, 2018: Reproduction in Domestic Animals, Zuchthygiene
Maryam Nosrati, Hojjat Asadollahpour Nanaei, Zeinab Amiri Ghanatsaman, Ali Esmailizadeh
Ovulation rate and prolificacy are the most important reproductive traits that have major impact on efficiency of lamb meat production. Here, we compared the whole genomes of the Romanov sheep, known as one of the high prolific breeds, and four other sheep breeds namely Assaf, Awassi, Combridge and British du cher, to identify genetic mechanisms underlying prolificacy in sheep. Selection signatures analysis revealed 637 and 477 protein-coding genes under positive selection from FST and nucleotide diversity (Pi) statistics, respectively...
October 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
H Pothmann, J Müller, I Pothmann, A Tichy, M Drillich
The objectives of this study were: (i) to assess the reproducibility of polymorphonuclear neutrophil (PMN) cell-counts at five pre-defined endometrial sites (corpus uteri, left horn base, right horn base, left horn tip, and right horn tip) and (ii) to determine the agreement for the diagnosis of subclinical endometritis (SE) between the different endometrial sites. Forty milking cows between 28 to 34 days postpartum were enrolled for endometrial sampling using cytobrush-technique. Intraclass correlation coefficient (ICC) was calculated to evaluate the reproducibility of PMN-counts at different sites...
October 24, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ziyad Al-Kass, J Spergser, Christine Aurich, Juliane Kuhl, Kathrin Schmidt, J M Morrell
Bacteria contaminate semen during collection and handling. The objective of this study was to identify the bacteria in pony stallion semen, the effects of antibiotics included in commercial semen extenders (Lincomycin and Spectinomycin), and the effect of modified single layer centrifugation (MSLC), on bacterial load. Ejaculates from six pony stallions, 3 ejaculates per animal, were extended in EquiPlus extender either with or without antibiotics. Aliquots were processed by MSLC to form four treatment groups: control and MSLC with antibiotics (CA and SA, respectively); and control and MSLC without antibiotics (CW and SW, respectively)...
October 23, 2018: Reproduction in Domestic Animals, Zuchthygiene
L M C Pereira, P R O Bersano, D D Rocha, M D Lopes
This study aimed to investigate the localization of proteins MPF, MAPK, p34cdc2 and cyclin B1, before and after treatment with EGF during different moments of oocyte maturation. The ovaries obtained from 350 domestic dogs were aseptically isolated, immersed in physiological solution and transported at 4°C. In the laboratory, the ovaries were sectioned for the release of cumulus-oocyte complexes (COCs). COCs were selected and divided into treatment groups with and without EGF and cultured for 24h, 48h and 72h...
October 23, 2018: Reproduction in Domestic Animals, Zuchthygiene
D A Nehru, G S Dhaliwal, M H Jan, R S Cheema, S Kumar
This study examined the effect of single IU administration of cephapirin on clinical recovery, clearance of uterine bacteria and reproductive performance of postpartum buffaloes with subclinical endometritis (SCE). Buffaloes (n=86) at 35 days postpartum (DPP) with >10% polymorphonuclear (PMN) cells in endometrial cytosmears were designated as positive (SCEP, n=29) and buffaloes with ≤10% PMN cell were designated as negative (SCEN, n=57) for SCE. Out of 29 positive buffaloes, 15 were administered a single intrauterine dose of cephapirin benzathine on 40 DPP (SCEP-CB), while the remaining 14 animals were kept as untreated control (SCEP-C)...
October 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Xuan Chen, Biao Xuan, Da Xu, Qiuyue Wang, Mimi Cheng, Jin Yi
The purpose of the present study was to assess the effect of crocin supplementation during oocyte maturation on the antioxidant defense and anti-apoptotic ability and subsequent developmental competence of porcine oocytes. Oocytes were cultured in media containing 0, 300, 400, or 500 ug/mL of crocin. Upon maturation, the maturation rates, reactive oxygen species (ROS) and glutathione (GSH) levels, mRNA expression of genes (SOD, CAT, GPx, Bcl-2, BAX and Caspase3), expression of cleaved caspase-3 and subsequent embryo cleavage rates were measured...
October 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Ebru Karakaya-Bilen, Gulnaz Yilmazbas Mecitoglu, Abdulkadir Keskin, Barıs Guner, Enes Serim, José E P Santos, Ahmet Gümen
The objective was to compare pregnancy per artificial insemination (P/AI) with conventional (CS) or sex-sorted (SS) semen in dairy cows subjected to one of the three timed AI protocols. Cows (n = 356) were randomly assigned to synchronization with Ovsynch (OVS), Presynch-Ovsynch (PO) or Double-Ovsynch (DO) and inseminated on Day 77 ± 3 postpartum with either frozen-thawed SS (n = 182) or CS (n = 184) of the same bull. More cows were cyclic at the beginning of breeding Ovsynch increased (p < .01) with presynchronization and it was greater for DO than PO (OVS = 78...
October 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Carolina Gonzales da Silva, Carlos Frederico Martins, Heidi Christina Bessler, Álvaro Moraes da Fonseca Neto, Tereza Cristina Cardoso, Maurício Machaim Franco, Anelise Dos Santos Mendonça, Ligiane de Oliveira Leme, José Renato Junqueira Borges, Juaci Vitoria Malaquias, Sônia Nair Báo
The aim of this work was investigate the methylation and hydroximethylation status of mesenchymal stem cells (MSC) from amniotic fluid (MSC-AF), adipose tissue (MSC-AT) and fibroblasts (FIB-control) and verify the effect of trichostatin A (TSA) on gene expression and development of cloned bovine embryos produced using these cells. Characterization of MSC from two animals (BOV1 and BOV2) was performed by flow cytometry, immunophenotyping and analysis of cellular differentiation genes expression. The cells were used in the nuclear transfer in the absence or presence of 50 nM TSA for 20 h in embryo culture...
October 14, 2018: Reproduction in Domestic Animals, Zuchthygiene
Jose A Len, David Beehan, Bruce Eilts, Sara K Lyle
To estimate when, during stallions' extra-gonadal reserves (EGR) depletion period, sperm quality would reach its highest quality, six light breed sexually rested stallions were collected daily for 7 days to deplete EGR. On collection days 1, 3, 5, and 7, sperm output, total (TM) and progressive (PM) motility, morphology, and plasma membrane (PLM) integrity were evaluated. Sperm output decreased as EGR depletion advanced, stabilizing on days 5-7. Sperm motility (TM and PM) and morphology were not different during EGR depletion...
October 13, 2018: Reproduction in Domestic Animals, Zuchthygiene
Patricia Steckeler, Frank Weber, Holm Zerbe, Anna Rieger, Katja Voigt
As sheep produce similar pregnancy-associated glycoproteins (PAGs) to cattle, a commercially available bovine visual pregnancy test based on the detection of PAGs (visual-PAG-test) was evaluated in sheep. The test was performed with whole blood (WhB), plasma (P) and serum (S) of 163 pregnant and 153 non-pregnant ewes. Additionally, 11 pregnant ewes were tested weekly from day 14 to 49 of gestation and monthly from day 60 to day 149. Ten ewes were sampled weekly from the date of lambing until day 63 post-partum (p...
September 29, 2018: Reproduction in Domestic Animals, Zuchthygiene
Tanja E Wolf, Abdi Arif, Nigel C Bennett, André Ganswindt
As the natural habitat of more and more species becomes depleted, captive breeding programmes have become established to bring species back from the brink of extinction. Monitoring the reproductive status of an individual is essential in order to improve breeding success. Traditional methods have involved stressful blood sampling, and thus noninvasive methods have been proven to be reliable alternatives for monitoring reproductive function in both captive and free-ranging animals. Subsequently, noninvasive methods have become an invaluable tool in longitudinal studies and conservation efforts, as animals can be observed without, or minimal human contact...
September 24, 2018: Reproduction in Domestic Animals, Zuchthygiene
Tasha R Gruhot, Lea A Rempel, Matthew L Spangler, Stephen D Kachman, Benny E Mote
Ultrasonography was used to capture a coronal-sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal-line boars at approximately 6 months of age at the University of Nebraska-Lincoln. Varicocele was diagnosed by two methods. Method 1 diagnosed varicocele as when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocele when the average vessel area on one side of the scrotum of a boar was 1...
September 24, 2018: Reproduction in Domestic Animals, Zuchthygiene
Maria Júlia B Bezerra, Mariana B Silva, Carlos H Lobo, Fábio R Vasconcelos, Marina D Lobo, Ana Cristina O Monteiro-Moreira, Renato A Moreira, Mariana Machado Neves, José R Figueiredo, Arlindo A Moura
Brazilian Somalis is a locally-adapted breed of rams raised in tropical climate and native pastures. The present study was conducted to evaluate gene expression and proteome of the reproductive tract of such rams. Samples were collected from testes, epididymides, seminal vesicles and bulbourethral glands of four rams. Expression of clusterin (CLU), osteopontin (OPN) and prostaglandin D2 synthase (PGDS) genes were evaluated in all samples by real-time PCR. Shotgun proteomic analysis was performed using samples from the head, corpus and cauda epididymides and from all other structures as well...
September 24, 2018: Reproduction in Domestic Animals, Zuchthygiene
Tiago S Nascimento, Isabelle S M Silva, Maria Cecília M A Alves, Bruna B Gouveia, Lara Mariane R Barbosa, Taís J S Macedo, Jamile M S Santos, Alane P O Monte, Maria Helena T Matos, Francine F Padilha, Isabel B Lima-Verde
This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high-performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml-HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species-ROS and glutathione-GSH) and active mitochondria...
September 23, 2018: Reproduction in Domestic Animals, Zuchthygiene
Abdul G Miah, Roslyn Bathgate, Koh-Ichi Hamano, Ummay Salma
The study was designed with three experiments to evaluate the effects of pre-freeze supplementation of Nigella sativa oil (NSO) and thymoquinone (TQ) on total motility, progressive motility, biokinetic characteristics, acrosomal integrity and DNA integrity of cryopreserved ovine spermatozoa. Semen samples collected from three proven fertile Merino rams were diluted with a Tris-based cryomedia containing different levels of NSO (Experiment I: 0, 10, 100 and 1,000 g/ml), TQ (Experiment II: 0, 1, 10 and 20 g/ml) and their optimum levels (Experiment III: 100 g/ml of NSO, 10 g/ml of TQ and 1 mM of α-tocopherol and cryopreserved as pellet (200µL) and subsequently evaluated at different post-thaw incubation periods (0, 2 and 4 hr)...
September 22, 2018: Reproduction in Domestic Animals, Zuchthygiene
Anna Domoslawska, Slawomir Zdunczyk, Monika Franczyk, Marta Kankofer, Tomasz Janowski
The aim of this study was to evaluate the total antioxidant capacity and protein peroxidation intensity in seminal plasma of infertile and fertile dogs. The study was conducted on 10 infertile and 10 fertile dogs of various breeds. Infertility was defined as conception failure at least 3 matings with different bitches. Semen was collected by manual manipulation. The sperm concentration and motility parameters were evaluated using CASA Hamilton Thorne, Vers. IVOS 12.3. The morphology of spermatozoa and the percentage of live and dead sperm cells were assessed microscopically, total antioxidant capacity and the content of SH-groups in seminal plasma were determined spectrophotometrically, the contents of protein peroxidation markers in seminal plasma, bityrosine and formylokinurenine, were determined using spectrofluorimetric methods...
September 17, 2018: Reproduction in Domestic Animals, Zuchthygiene
Mahmoud S El-Tarabany, Khaled M Al-Marakby
The objectives were to evaluate the reproductive indices and survival analysis of pregnancy outcomes in multiparous repeat breeder Holstein cows (n = 557). The cows were synchronized to ovulate by Ovsynch, new controlled internal drug release device (N-CIDRsynch), and once-used CIDR device (U-CIDRsynch). The pregnancy per AI at 28 days post-insemination (P/AI 28) in the N-CIDRsynch group (28.75%) was significantly (COR = 1.49; p = 0.011) greater than that reported in the Ovsynch (23.46%) and U-CIDRsynch (21...
September 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
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