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Reproduction in Domestic Animals, Zuchthygiene

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https://www.readbyqxmd.com/read/28211166/clinical-relevance-of-pre-ovulatory-follicular-temperature-in-heat-stressed-lactating-dairy-cows
#1
F López-Gatius, Rhf Hunter
Temperature gradients in female reproductive tissues seem to influence the success of key processes such as ovulation and fertilization. The objective of this study was to investigate whether pre-ovulatory follicles are cooler than neighbouring uterine tissue and deep rectal temperatures in lactating dairy cows under heat stress conditions. Temperatures within the pre-ovulatory follicle, on the uterine adjacent surface and 20 cm deep within rectum, were measured using fine thermistor probes within 45 min after sunrise (dawn)...
February 17, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28211113/distribution-of-mast-cells-in-the-feline-ovary-in-various-phases-of-the-oestrous-cycle
#2
P Hamouzova, P Cizek, R Novotny, A Bartoskova, F Tichy
This study is the first description of the distribution of mast cells in various phases of the oestrous cycle in the ovary of cat. Furthermore, this is the first description in species with an induced ovulation. The aim was to describe the distribution of mast cells and variability of their numbers in the feline ovaries in different phases of the oestrous cycle. The number of mast cells in medulla ovarii was affected by the estradiol and progesterone level in the blood serum because the lowest number was detected in anoestrus when the levels of hormones were basal...
February 17, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28191700/supplement-of-autologous-ooplasm-into-porcine-somatic-cell-nuclear-transfer-embryos-does-not-alter-embryo-development
#3
W-J Lee, J-H Lee, R-H Jeon, S-J Jang, S-C Lee, J-S Park, S-L Lee, W-A King, G-J Rho
Somatic cell nuclear transfer (SCNT) is considered as the technique in which a somatic cell is introduced into an enucleated oocyte to make a cloned animal. However, it is unavoidable to lose a small amount of the ooplasm during enucleation step during SCNT procedure. The present study was aimed to uncover whether the supplement of autologous ooplasm could ameliorate the oocyte competence so as to improve low efficiency of embryo development in porcine SCNT. Autologous ooplasm-transferred (AOT) embryos were generated by the supplementation with autologous ooplasm into SCNT embryos...
February 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28191666/appraisal-and-standardization-of-curvilinear-velocity-vcl-cut-off-values-for-casa-analysis-of-japanese-quail-coturnix-japonica-sperm
#4
U Farooq, I A Malecki, M Mahmood, G B Martin
One of the basic steps in objective analysis of sperm motility is the subdivision of a motile sperm population into slow, medium and rapid categories based on their velocity. However, for CASA analysis of quail sperm, the velocity values for categorization of slow, medium and rapid sperm have not yet been standardized. To identify the cut-off values of "velocity curvilinear" (VCL) for quail sperm categorization, we captured and analysed 22,300 tracks of quail sperm using SCA(®) -CASA. The median and mean VCL values were 85 and 97 μm/s...
February 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28181328/protein-and-mrna-expression-of-follicle-stimulating-hormone-receptor-and-luteinizing-hormone-receptor-during-the-oestrus-in-the-yak-bos-grunniens
#5
S-D Huo, S-E Chen, R-J Long, J-T Yang, J-X Lu, R-X Zang, T-J Zhang, A Abudureyimu, J-L Liu, G-H Zhang, Y-Q Zhao, Z-R Ma
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have a central role in follicle growth, maturation and oestrus, but no clear pathway in the seasonal oestrus of yak (Bos grunniens) has been found. To better understand the role of FSH and LH in seasonal oestrus in the yak, six yaks were slaughtered while in oestrus, and the pineal gland, hypothalamus, pituitary gland, and gonads were collected. Using real-time PCR and immunohistochemical assays, we determined the mRNA and protein expression of the FSH and LH receptors (FSHR and LHR) in these organs...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28181320/are-foetal-ultrasonographic-and-maternal-blood-progesterone-measurements-near-parturition-reliable-predictors-of-the-time-of-birth-in-the-domestic-cat
#6
R Keiser, I M Reichler, O Balogh
In cats, accuracy of parturition day prediction by ultrasonographic measurement of foetal structures is decreasing towards the end of gestation. Foetal measurements during the last days of pregnancy are scarce. We determined foetal biparietal, abdominal and eye diameter (BPD, AD and ED, respectively) by ultrasonography as well as maternal blood progesterone (P4) within five days of delivery to predict parturition date and calculate accuracy of prediction. Foetal BPD at birth was compared with newborn kitten head diameter (HD)...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28181310/localization-of-orexin-b-and-receptor-2-for-orexins-in-testicular-cytotypes-of-the-camelid-alpaca-vicugna-pacos
#7
G Liguori, C Squillacioti, L Assisi, N Mirabella, E Langella, A Costagliola, A Vittoria
The orexins A (OxA) and B (OxB) are two hypothalamic peptides involved in many physiological functions of the mammalian body. They act through the binding of two G-coupled receptors named receptor 1 (OX1 ) and receptor 2 (OX2 ) for orexins. The first receptor is specific for OxA, while the second binds both the substances with equal affinity. The orexins and the relative receptors have been traced by means of different techniques also at the periphery of the body and particularly in the adrenals, and in gastrointestinal and genital organs...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28181308/administration-of-exogenous-hormones-in-ovulatory-and-embryonic-response-in-pelibuey-sheep
#8
A García-Salas, C Cortez-Romero, J Salazar-Ortiz, J Arroyo-Ledezma, V M Ruíz-Vera, H Vaquera-Huerta, J Gallegos-Sánchez
The objective of this study was to evaluate the effect of two sources of commercial porcine pituitary-derived follicle-stimulating hormone (pFSH) and pFSH-porcine Luteinizing Hormone (pLH), including equine chorionic gonadotropin (eCG), in ovulatory and embryonic response in Pelibuey sheep. Twenty-four Pelibuey sheep were used and were assigned randomly to four treatments (n = 6): (T1; 200 mg pFSH-Folltropin(®) ); (T2; 200 mg pFSH + 300 UI eCG-Folligon(®) ); (T3; 250 UI pFSH/pLH-Pluset(®) ) and (T4; 250 UI pFSH/pLH + 300 UI eCG)...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28176427/uterine-serpin-serpina-14-correlates-negatively-with-cytokine-production-at-the-foetal-maternal-interface-but-not-in-the-corpus-luteum-in-pregnant-dairy-heifers-experimentally-infected-with-neospora-caninum
#9
B Serrano-Pérez, S Almería, R Mur-Novales, I López-Helguera, I Garcia-Ispierto, J L Alabart, L Darwich, F López-Gatius
This study examines gene expression patterns in dairy heifers experimentally infected with N. caninum during on Day 110 of pregnancy with live foetuses at euthanasia, 42 days later. The study population was constituted of four non-infected controls and three infected dams. Gene expression was determined on gamma interferon (IFNγ), (Th1 pro-inflammatory cytokine), interleukin-4 (IL4) (Th2 pro-gestation cytokine) or interleukin-10 (IL10) (T regulatory cytokine) and the serine peptidase inhibitor SERPINA14 in intercaruncular, placental, uterine lymph node (UTLN) and luteal tissue samples...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28176388/urine-levels-of-luteinizing-hormone-as-predictor-of-the-period-of-ovulation-for-advantage-of-timed-artificial-insemination-in-murrah-buffalo-bubalus-bubalis
#10
R M Selvam, D Singh, M A Akbarsha, G Archunan
Assessment of urine levels of luteinizing hormone (LH) for predicting the reproductive status of animals is in practice. The aim of this study was to predict the period of ovulation based on the urine levels of LH for timed-artificial insemination to increase the conception rate in buffaloes, which are naturally silent-oestrous animals. Level of LH in urine was assessed using ELISA, and a cut-off LH concentration for prediction of ovulation period was obtained using receiver operating characteristic analysis...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28176384/the-effect-of-some-cryoprotectants-on-dromedary-camel-frozen-thawed-semen
#11
D A El-Badry, R H Mohamed, H A El-Metwally, T R Abo Al-Naga
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh-G), dimethyl formamide (DMF, Sh-DF), dimethyl sulfoxide (DMSO, Sh-DS) or ethylene glycol (EG, Sh-EG), all at 6% final concentration, and the samples were subjected to cryopreservation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28176378/effects-of-supplemental-conjugated-linoleic-acids-cla-on-fresh-and-post-thaw-sperm-quality-of-holstein-bulls
#12
R Karimi, A Towhidi, S Zeinoaldini, K Rezayazdi, M Mousavi, H Safari, F Martinez-Pastor
This study was designed to investigate the effects of feeding-protected conjugated linoleic acid (CLA) on the semen production and sperm freezability in Holstein bulls. Twelve bulls were randomly assigned to two groups (n = 6 per group). Bulls received the normal diet (control group) or the normal diet top-dressed with 50 g of CLA (treated group) for 10 weeks. The control group received 40 g/day calcium soap of fatty acid. Fresh and post-thaw semen quality was assessed on ejaculates collected at the 0, 4, 6, 8 and 10 week of supplementation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28120512/ejaculation-does-not-contribute-to-the-stress-response-to%C3%A2-electroejaculation-in-sheep
#13
S Abril-Sánchez, A Freitas-de-Melo, J P Damián, J Giriboni, A Villagrá-García, R Ungerfeld
Electroejaculation procedures (EEPs) provoke stress; nevertheless, ejaculation produces physiological changes similar as those usually used to measure stress responses. The application of EEP to animals that cannot ejaculate-as ewes-may be useful to discriminate the responses induced by ejaculation from those provoked by EEP. The aim was to determine the stress response to EEP in rams and ewes. The EEPs were applied to 10 rams and 10 ewes during the non-breeding season, and the number of vocalizations, the heart rate, rectal temperature, serum cortisol concentration, biochemical and haematological parameters were measured...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28120399/the-effect-of-cooling-to-different-subzero-temperatures-on-dog-sperm-cryosurvival
#14
A Alcantar-Rodriguez, A Medrano
The objective was to assess the effect of cooling to different subzero temperatures around ice formation (-5°C) on dog sperm cryosurvival and plasma membrane fluidity. Semen was centrifuged, and sperm were resuspended in a Tris-egg yolk medium (3% glycerol). Diluted sperm were cooled from 22 to 5°C, and then, a Tris-egg yolk medium containing 7% glycerol was added (final concentration of 5% glycerol and 200 × 10(6)  cells/ml). Sperm were packaged in 0.5-ml plastic straws, and equilibration was done 16 hr at 5°C before freezing...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28120355/transporting-bovine-oocytes-in-a-medium-supplemented-with-different-macromolecules-and-antioxidants-effects-on-nuclear-and-cytoplasmic-maturation-and-embryonic-development-in-vitro
#15
M Ambrogi, P C Dall'Acqua, Nas Rocha-Frigoni, Bcs Leão, G Z Mingoti
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox)...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28120353/analysis-of-gene-expression-in-granulosa-cells-post-maturation-to-evaluate-oocyte-culture-systems-in-the-domestic-cat
#16
J Zahmel, H Mundt, K Jewgenow, B C Braun
Maturation of oocytes is a prerequisite for successful embryo development. The fertilization competence of in vivo derived oocytes is significantly higher than that of oocytes matured in vitro. Commonly evaluated morphological criteria for oocyte maturation do not reflect the complexity and quality of maturation processes. Oocytes and granulosa cells are communicating closely in a bidirectional way during follicular growth and maturation. Assessing the mRNA expression of specific genes in granulosa cells could be a non-invasive way to evaluate the conditions of in vitro oocyte maturation...
January 24, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28111814/testosterone-serum-profile-semen-characteristics-and-testicular-biometry-of-mangalarga-marchador-stallions-in-a-tropical-environment
#17
B Waddington, J M Penitente-Filho, Jgs Neves, R O Pinho, A Y Chaya, P P Maitan, C O Silveira, M G Neves, Sef Guimarães, G R de Carvalho, J D Guimarães
This study was conducted to characterize the daily profile of testosterone secretion and its mean concentrations in the four seasons as well as to evaluate the semen characteristics and testicular biometry of Mangalarga Marchador stallions throughout the year in a tropical region. Three stallions were submitted to semen collections and evaluation of testicular biometry every 14 days along a year. Blood samples were collected once at the middle of each season, in a 20-min interval during 24 hr in order to evaluate the testosterone secretion profiles among seasons...
January 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28111796/establishment-and-characterization-of-a-coculture-system-of-equine-endometrial-epithelial-and-stromal-cells
#18
L Lapko, D Böttcher, T Theuß, J Klug, H-A Schoon
To investigate the equine endometrium as close to the in vivo situation as possible, we established a coculture system for epithelial and stromal cells (ECs/SCs). ECs and SCs were isolated from nine endometrial tissue specimens. ECs obtained as glandular formations were cultivated on one side of the semipermeable membrane of a Millicell(®) insert. After 2 days, SCs (2 × 10(4) cells/membrane) were seeded onto the other side of the same membrane. During cocultivation, the low serum containing culture medium (Theuß et al...
January 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28111812/dynamic-changes-in-mitochondrial-dna-distribution-and-activity-within-cat-oocytes-during-folliculogenesis
#19
N Songsasen, L H Henson, W Tipkantha, C Thongkittidilok, J H Henson, K Chatdarong, P Comizzoli
Mitochondria play fundamental roles during oocyte development. The accumulation and spatial redistribution of these energy-producing organelles have been linked to the developmental competence of mammalian oocytes. Here, we assessed the copy number, distribution and activity of mitochondria within cat oocytes during folliculogenesis. In Experiment 1, oocytes were recovered from primordial (n = 152), primary (112), secondary (95), early (131), small (118), antral (86) and advanced antral (5) stages follicles, and mitochondria DNA extracted and quantified using qPCR...
January 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28111808/metabolism-of-prostaglandin-f2alpha-in-eurasian-lynx-lynx-lynx-and-asian-leopard-cat-prionailurus-bengalensis-euptilura
#20
M Dehnhard, S V Naidenko, K Jewgenow
Methods for monitoring endocrine status are useful tools for reproduction management. In particular, successful captive breeding of endangered feline species requires reliable methods for pregnancy diagnosis. In many species, uterine and placental prostaglandin-F2α (PGF2α) is involved in the regulation of reproductive processes. PGF2α is metabolized to 13,14-dihydro-15-keto-PGF2a (PGFM) during the first passage through the lungs. Immunoreactive PGFM is elevated in pregnant felids during the last trimester and is used for pregnancy diagnosis, although authentic PGFM is excreted in negligible amounts...
January 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
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