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Journal of Steroid Biochemistry and Molecular Biology

David Scott, Aya Mousa, Negar Naderpoor, Maximilian P J de Courten, Robert Scragg, Barbora de Courten
Recent trials do not support a role for vitamin D supplementation in prevention or treatment of type 2 diabetes mellitus, although effects may differ in Asian populations. In this pilot secondary analysis of a placebo-controlled randomised trial of overweight or obese individuals with low 25-hydroxyvitamin D (25(OH)D<50 nmol/L), we examined whether vitamin D supplementation improved insulin sensitivity or body composition in participants of Asian ethnicity. Amongst 65 trial participants, 33 reported being of Asian descent (mean ± SD age 30 ± 7 years; 67% male)...
October 12, 2018: Journal of Steroid Biochemistry and Molecular Biology
Constantinos Potamitis, Dimitra Siakouli, Konstantinos D Papavasileiou, Athina Boulaka, Vassiliki Ganou, Marina Roussaki, Theodora Calogeropoulou, Panagiotis Zoumpoulakis, Michael N Alexis, Maria Zervou, Dimitra J Mitsiou
Glucocorticoids (GCs) are widely used as potent anti-inflammatory drugs; however, GC therapy is often accompanied by adverse side effects. The anti-inflammatory action of GCs is exerted through the glucocorticoid receptor (GR) in part by antagonizing the pro-inflammatory nuclear factor k B (NF-kB) whereas the majority of side effects are assumed to be mediated by transactivation of GR target genes. We set out to identify novel non-steroidal selective GR agonists (SEGRA) favoring transrepression of NF-kB target genes over transactivation of genes associated with undesirable effects...
October 12, 2018: Journal of Steroid Biochemistry and Molecular Biology
Alejandra Suares, Cinthya Tapia, Verónica González-Pardo
The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor (KSHV/vGPCR) is a key molecule in the pathogenesis of Kaposi's sarcoma. In endothelial cells, tumor maintenance and NF-κB activation depends on vGPCR constitutive expression and activity. We have previously demonstrated that 1α,25(OH)2 D3 induces apoptosis in a VDR dependent manner, inhibits vGPCR cell growth and NF-κB activity. In this study, we developed a method to obtain multicellular spheroids (MCS) from endothelial cells expressing vGPCR in order to test whether MCS have a similar response to 2D-cultures after 1α,25(OH)2 D3 treatment...
October 9, 2018: Journal of Steroid Biochemistry and Molecular Biology
Meabh Walsh, Linnea Bärebring, Hanna Augustin
BACKGROUND: Vitamin D status is hypothesised to play a role in gestational glucose control. No studies to date have examined vitamin D in relation to changes in blood glucose in pregnancy. Thus, the aim was to examine if vitamin D in early pregnancy and vitamin D trajectory associate with blood glucose trajectory over pregnancy in a Swedish cohort. We also investigated the relation between maternal vitamin D status and excessive fetal growth. METHODS: In 2013-2014, pregnant women were recruited to the GraviD cohort study when registering at the antenatal clinics in south-west Sweden...
October 8, 2018: Journal of Steroid Biochemistry and Molecular Biology
Simon Ghaly, Dana Bliuc, Jacqueline Centre, Michael W Clarke, Anderson P Jones, Stephanie Trend, Allan G Kermode, Rachel E Neale, Prue H Hart
A proportion of circulating 25-hydroxy vitamin D3 (25(OH)D3) ) undergoes epimerization to form C3-epi 25(OH)D3 and C3-epi 1,25(OH)2 D3 . These epimers have less calcaemic activity than non-epimerized metabolites and are not differentiated by many immunoassays when reporting total 25(OH)D3 levels. This study aimed to compare the effect of exposure to ultraviolet radiation (UVR) and oral vitamin D3 supplementation on vitamin D C3-epimer levels. C57Bl/6 female mice were fed either vitamin D-sufficient (vitamin D3 2000 IU/kg) or -deficient diets (no vitamin D3 ) for 4 weeks...
October 5, 2018: Journal of Steroid Biochemistry and Molecular Biology
Malachi J McKenna, Barbara Murray, Rachel K Crowley, Patrick J Twomey, Mark T Kilbane
Serum 25-hydroxyvitamin D (25OHD) is the pre-eminent estimate of vitamin D status that we have been measuring in a hospital laboratory setting since the 1970s. We previously evaluated the trend in 25OHD results in our laboratory from 1993 to 2013. Using a time series analysis of monthly average 25OHD results the trend was modelled, and this was used to forecast monthly average 25OHD from 2014 to 2016. In this study, all 25OHD results from 2014 to 2016 were retrieved (n = 67,922) and trimmed to 40,307 results after duplicates were excluded...
October 5, 2018: Journal of Steroid Biochemistry and Molecular Biology
C Ventura, C D Zappia, M Lasagna, W Pavicic, S Richard, A D Bolzan, F Monczor, M Núñez, C Cocca
Chlorpyrifos (CPF) is an organophosphorus pesticide used for agricultural pest control all over the world. We have previously demonstrated that environmental concentrations of this pesticide alter mammary gland histological structure and hormonal balance in rats chronically exposed. In this work, we analyzed the effects of CPF on mammary tumors development. Our results demonstrated that CPF increases tumor incidence and reduces latency of NMU-induced mammary tumors. Although no changes were observed in tumor growth rate, we found a reduced steroid hormone receptor expression in the tumors of animals exposed to the pesticide...
October 2, 2018: Journal of Steroid Biochemistry and Molecular Biology
Yuta Terauchi, Rie Suzuki, Ryosuke Takeda, Ittetsu Kobayashi, Atsushi Kittaka, Midori Takimoto-Kamimura, Noriyuki Kurita
Vitamin D is recognized to play important roles in the onset of immunological diseases as well as the regulation of the amount of Ca in the blood. Since these physiological actions caused by active vitamin D are triggered by the specific interaction between the vitamin D receptor (VDR) and active vitamin D, many types of compounds have been developed as potent ligands against VDR. It was found that the binding affinity between VDR and its ligand depends significantly on the chirality of the ligand. However, the reason for the dependence has, thus far, not been elucidated...
September 29, 2018: Journal of Steroid Biochemistry and Molecular Biology
Cora M Best, Eva K Pressman, Ruth Anne Queenan, Elizabeth Cooper, Kimberly O O'Brien
Serum free 25-hydroxyvitamin D (25(OH)D) rather than total 25(OH)D may better indicate vitamin D status during pregnancy given the pregnancy-associated increase in serum vitamin D binding protein (DBP) concentration. Our aims were to assess changes in DBP and free 25(OH)D across gestation and to determine whether free compared with total 25(OH)D more strongly correlates with markers of vitamin D and calcium metabolism during pregnancy. This ancillary study included 58 pregnant adolescents (53% African American, 47% White) who completed a vitamin D3 supplementation study in Rochester, NY...
September 29, 2018: Journal of Steroid Biochemistry and Molecular Biology
Andrea Bileck, Christa E Fluck, Nasser Dhayat, Michael Groessl
Due to possible matrix interferences and artefact generation during sample preparation, careful method validation is required for quantitative bioanalytical methods, especially for analytes that are only present in low concentrations. Using the identification and quantification of progesterone metabolites in the urine of newborns as an example, we show how modern high-resolution instruments can be used to verify analyte assignment and avoid pitfalls commonly encountered by the use of low-resolution instruments...
September 27, 2018: Journal of Steroid Biochemistry and Molecular Biology
Tea Lanisnik Rizner, Jerzy Adamski
No abstract text is available yet for this article.
September 24, 2018: Journal of Steroid Biochemistry and Molecular Biology
Irfan Ahmad Bhat, Irshad Ahmad, Ishfaq Nazir Mir, Raja Aadil Hussain Bhat, Gireesh-Babu P, Mukunda Goswami, Sundaray J K, Rupam Sharma
The study was undertaken to explore the molecular mechanism of eurycomanone, a major compound of Eurycoma longifolia plant in increasing the reproductive processes in the male fish model. Chitosan-nanoconjugated eurycomanone nanoparticles with a significant particle size [130 nm (CED1); 144.1 nm (CED2)] and stable zeta potentials (+49.1 mV and +30 mV) were synthesized and evaluated against naked eurycomanone (ED1 and ED2). In present study, short-term and long-term experiments were conducted to evaluate the effect of nano-formulation on expression of endocrine-related genes, circulating hormone concentrations (Follicle stimulating hormone, FSH; luteinizing hormone, LH; progesterone, testosterone and 17-β estradiol) and reproductive capacity of male Clarias magur...
September 22, 2018: Journal of Steroid Biochemistry and Molecular Biology
Wei Wang, Guo-Dong Zhao, Ying-Jie Cui, Min-Qi Li, Zhao-Peng Liu
Two novel 1α,25-dihydroxyvitamin D3 derivatives containing a α,α-difluorocyclopentanone (3) or α,α-difluorocyclohexanone (4) moiety at the CD-ring side chains were designed, synthesized, and evaluated for their biological properties on restoring bone mass in ovariectomized (OVX) rats with established osteopenia. The synthesis of compounds 3 and 4 utilized the Wittig-Horner coupling to build up the vitamin D conjugated triene system, followed by the introduction of the cycloketone fragments at the side chain, and subsequent α,α-difluorination of the ketone by the treatment of the derived silyl enol ether with Selectfluor, as the key synthetic steps...
September 22, 2018: Journal of Steroid Biochemistry and Molecular Biology
Marco Bauzá-Thorbrügge, Sergio Rodríguez-Cuenca, Antonio Vidal-Puig, Bel M Galmés-Pascual, Miquel Sbert-Roig, Magdalena Gianotti, Isabel Lladó, Ana M Proenza
Obesity is associated with inflammation, dysregulated adipokine secretion, and disrupted adipose tissue mitochondrial function. Estradiol (E2) has been previously reported to increase mitochondrial function and biogenesis in several cell lines, but neither the type of oestrogen receptor (ERα, ERβ and GPER) involved nor the mechanism whereby such effects are exerted have been fully described. Considering the anti-inflammatory activity of E2 as well as its effects in enhancing mitochondrial biogenesis, the aim of this study was to investigate the contribution of ERα, ERβ, and GPER signaling to the E2-mediated enhancement of adipocyte mitochondrial function in a pro-inflammatory situation...
September 22, 2018: Journal of Steroid Biochemistry and Molecular Biology
Adrian Fabisiak, Pawel Brzeminski, Klaudia Berkowska, Ewa Marcinkowska, Rafal R Sicinski
The results presented in this paper constitute an extension of our synthetic efforts focused on 19-norvitamin D compounds possessing elongated 2-alkylidene substituents. Based on our previous results, molecular modeling studies, and docking experiments, we selected a novel 19-norcalcitriol analog with long chain at C-2 containing several ether moieties and terminated by 2-(pyridin-2'-yl)ethylamino fragment. It was expected that such structural modification might allow binding of transition metal by the ligand, increase solubility of the formed complexes as well as improve their affinity to the VDR...
September 20, 2018: Journal of Steroid Biochemistry and Molecular Biology
Anna Więch, Magdalena Rowińska-Żyrek, Joanna Wątły, Aleksandra Czarnota, Rafał Hołubowicz, Zbigniew Szewczuk, Andrzej Ożyhar, Marek Orłowski
The dominant vector of dengue and Zika diseases is a female Aedes aegypti mosquito. Its reproduction is controlled by the formation of an active heterodimer complex of the 20-hydroxyecdysone receptor (EcR) and Ultraspiracle protein (Usp). Although EcR exhibits a structural and functional organization typical of nuclear receptors (NRs), the EcR C-terminus has an additional F domain (AaFEcR) that is rarely present in the NRs superfamily. The presence of F domains is evolutionarily not well conserved in the NRs...
September 20, 2018: Journal of Steroid Biochemistry and Molecular Biology
Rita Sigüeiro, Julian Loureiro, Patricia González-Berdullas, Antonio Mouriño, Miguel A Maestro
As part of our program on synthesis of labeled vitamin D metabolites and analogs, we describe here an efficient and versatile synthetic approach to 28,28,28-trideutero- 25-hydroxydihydrotachysterol2 where isotopic labeling was incorporated stereoselectively in the last step of the synthesis. This deuterated compound will allow the study this analog in vitro or in vivo and to measure AT10-like compounds in serum by LC-MS/MS.
September 19, 2018: Journal of Steroid Biochemistry and Molecular Biology
Carmen J Reynolds, Nicholas J Koszewski, Ronald L Horst, Donald C Beitz, Jesse P Goff
1,25-Dihydroxyvitamin D3 (1,25(OH)2 D) elicits a transcriptional response in the intestines. Assessments of this response are often derived from crude tissue homogenates and eliminate the ability to discriminate among different cell types. Here, we used an RNA in situ hybridization assay, RNAScope (Advanced Cell Diagnostics, Newark, CA), to identify the cells in the intestine that respond to 1,25(OH)2 D with expression of cytochrome P450 family 24 subfamily A member 1 (Cyp24a1) mRNA. Mice were gavaged with a single bolus dose of 1,25(OH)2 D to target the duodenum or a glucuronic acid conjugate of 1,25(OH)2 D, β-G-1,25(OH)2 D, to target the colon...
September 17, 2018: Journal of Steroid Biochemistry and Molecular Biology
Jean-François Thériault, Sheng-Xiang Lin
Human 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 and 7 catalyze the final step of estrogen activation and the first step in androgen inactivation. It has been shown in breast cancer cells that DHT has a suppression effect on cell proliferation, counteracting the estrogen growth effect. However, the exact kinetic function of 17β-HSD7 in steroidogenesis was not determined. Here we report the steady-state kinetics and binding study for 17β-HSD7 with estrone or DHT as substrates and NADPH as cofactor...
September 15, 2018: Journal of Steroid Biochemistry and Molecular Biology
Lalrawngbawli Annie, Guruswami Gurusubramanian, Vikas Kumar Roy
Visfatin is an adipokine which has an endocrine effect on reproductive functions and regulates ovarian steroidogenesis. There is scant information about the expression, regulation, and functions of visfatin in the mammalian uterus. The present study examined expression and localization of visfatin in the mouse uterus at various stages of the natural estrous cycle, effects of estrogen and progesterone on localization and expression of visfatin in the ovariectomised mouse uterus and effect of visfatin inhibition by a specific inhibitor, FK866 on proliferation and apoptosis in the uterus...
September 15, 2018: Journal of Steroid Biochemistry and Molecular Biology
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