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International Journal of Experimental Pathology

Michelle Meier, Kasper Jarlhelt Andersen, Anders Riegels Knudsen, Jens Randel Nyengaard, Stephen Hamilton-Dutoit, Frank Viborg Mortensen
In this study we investigated the dynamics of hepatocyte hyperplasia and hypertrophy in rats subjected to increasing sizes of partial hepatectomy (PH). A total of 104 rats were randomized according to the size of PH. On postoperative days (PODs) 1, 3 and 5, blood was drawn and the remnant liver removed for stereological analysis. Liver parameters and regeneration rate were significantly affected by size of PH. On POD 1, hepatocyte volumes had increased significantly in all PH groups. On POD 3, all groups showed hepatocyte volumes approximating baseline...
September 10, 2018: International Journal of Experimental Pathology
Laura Colín-Barenque, Patricia Bizarro-Nevares, Adriana González Villalva, Jose Pedraza-Chaverri, Omar Noel Medina-Campos, Ruben Jimenez-Martínez, Daniela S Rodríguez-Rangel, Stefanie Reséndiz, Teresa I Fortoul
Carnosine (β-alanyl-L-histidine) is synthesized in the olfactory system, has antioxidant activity as a scavenger of free radicals and has been reported to have neuroprotective action in diseases which have been attributed to oxidative damage. In neurodegenerative disorders, such as Parkinson's and Alzheimer's diseases, impairment of olfactory function has been described. Vanadium derivatives are environmental pollutants, and its toxicity has been associated with oxidative stress. Vanadium toxicity on the olfactory bulb was reported previously...
September 9, 2018: International Journal of Experimental Pathology
Peng Zhang, Changsheng Fan, Jun Du, Xueli Mo, Qikang Zhao
Our study aimed to clarify the correlation between miR-1247-5p expression and clinicopathological parameters and survival of patients with breast cancer (BC). We evaluated the expression level of miR-1247-5p in 224 formalin-fixed, paraffin-embedded specimens (112 BC and matched cancer free tissues) by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). miR-1247-5p expression in BC tissues was found to be decreased compared with matched normal tissues (P < 0.01). Additionally, low miR-1247-5p expression in BC tissues was significantly associated with the advanced TNM stage (P = 0...
September 3, 2018: International Journal of Experimental Pathology
Airton Pereira E Silva, João R A Soares, Erika Bertozzi de Aquino Mattos, Claudia Josetti, Isabelle M Guimarães, Sylvia M N Campos, Gerlinde A P B Teixeira
Gut-associated intestinal lymphoid tissue, the largest secondary lymphoid organ in the human body, constantly samples antigens from the gut lumen, presenting as a default response the activation of TCD4+ FOXP3+ regulatory T cells that secrete a profile of anti-inflammatory cytokines maintaining gut homeostasis denominated from an immunological perspective as mucosal tolerance. However, when antigens are sampled in an inflammatory setting, the immune response may either be protective, in the case of harmful pathogens, or cause further inflammatory reactions as in food allergy, inflammatory bowel diseases, coeliac disease or food protein-induced enterocolitis syndrome...
September 2, 2018: International Journal of Experimental Pathology
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June 2018: International Journal of Experimental Pathology
Wang Qiong, Gu Xiaofeng, Wang Junfang
Precartilaginous stem cells (PSCs) are adult stem cells which could self-renew or differentiate into chondrocytes to promote bone growth. In this study, we aimed to understand the role of transforming growth factor-β1 (TGF-β1) in precartilaginous stem cell (PSC) differentiation and to study the mechanisms that underlie this role. We purified PSCs from the neonatal murine perichondrial mesenchyme using immunomagnetic beads, and primary cultured them. Their phenotype was confirmed by the PSC marker fibroblast growth factor receptor-3 (FGFR-3) overexpression...
June 2018: International Journal of Experimental Pathology
Ana Katusic Bojanac, Srdjan Rogosic, Nino Sincic, Gordana Juric-Lekic, Maja Vlahovic, Ljiljana Serman, Davor Jezek, Floriana Bulic-Jakus
We screened for the impact of hyperthermal regimes varying in the cumulative equivalent minutes at 43°C (CEM43°C) and media composition on tumour development using an original teratoma in vitro model. Rat embryos (three germ layers) were microsurgically isolated and cultivated at the air-liquid interface. During a two week period, ectodermal, mesodermal and endodermal derivatives developed within trilaminar teratomas. Controls were grown at 37°C. Overall growth was measured, and teratoma survival and differentiation were histologically assessed...
June 2018: International Journal of Experimental Pathology
Zayani Nesrine, Hamdouni Haithem, Boumaiza Imen, Neffati Fadoua, Omezzine Asma, Najjar Mohamed Fadhel, Bouslama Ali
Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Leptin (LEP). The aim this study was to investigate the prevalence of single nucleotide polymorphisms in LEP gene (LEP 3'UTR A/C, -2548 G/A) and LEPR (K109R and Q223R) and their association with Leptin level and obesity. We recruited 169 non-obese (body mass index [BMI] = 24.51-3.69 kg/m2 ) and 160 obese (BMI = 36-4.78 kg/m2 ) patients. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism, BMI was calculated, and Leptin level was measured by ELISA...
June 2018: International Journal of Experimental Pathology
Malcolm R Alison
In the latter half of the 20th century, our understanding of mammalian liver regeneration was shaped by the manner of compensatory hyperplasia occurring after a partial rat liver resection. This response involves almost all hepatocytes and thus is unlikely to be the outcome of the multiple cycling of a small stem cell population. It was most intense in the outer third of lobule, the location closest to the afferent arterial blood supply. With the advent of heritable genetic labelling techniques, usually applied to mice, hitherto unrecognized hepatocytes with clonogenic potential have been discovered, contributing to homoeostatic renewal and/or regenerative responses after tissue loss...
June 2018: International Journal of Experimental Pathology
Ana Paula Franco Punhagui, Giovana Rampazzo Teixeira, Marcelo Conrado de Freitas, Patricia Monteiro Seraphim, Glaura Scantamburlo Alves Fernandes
Obesity and absence of physical exercise are global problems that affect concentration and sperm quality in the male reproductive system. The purpose of this study was to examine the effect of obesity and resistance training, considered separately or in association, on testicular function and reproductive capacity. Twenty pubertal male Wistar rats were distributed into four groups: control (C) and exercise (E) groups that received standard rat chow; and obese (O) and obese with exercise (OE) groups that received a high-fat diet...
April 2018: International Journal of Experimental Pathology
Elysse C Filipe, Jessica L Chitty, Thomas R Cox
The extracellular matrix (ECM) is present in all solid tissues and considered a master regulator of cell behaviour and phenotype. The importance of maintaining the correct biochemical and biophysical properties of the ECM, and the subsequent regulation of cell and tissue homeostasis, is illustrated by the simple fact that the ECM is highly dysregulated in many different types of disease, especially cancer. The loss of tissue ECM homeostasis and integrity is seen as one of the hallmarks of cancer and typically defines transitional events in progression and metastasis...
April 2018: International Journal of Experimental Pathology
Guoxi Zhang, Xiaofeng Zou, Quanliang Liu, Tianpeng Xie, Ruohui Huang, Huan Kang, Changfu Lai, Jiaxing Zhu
The mechanism of aldosterone-producing adrenocortical adenoma (APA) pathogenesis and the role of microRNAs (miRNAs) in APA pathogenesis have not been completely clarified. We examined the expression and function of miR-140-3p, miR-193a-3p and miR-22-3p, which have binding sites in CYP11B2. Expression of miRNAs and CYP11B2 mRNA was measured by quantitative reverse transcription PCR (qRT-PCR). Cell proliferation was monitored by colorimetric analysis, and cell apoptosis and cell cycle progression were analysed by flow cytometry...
April 2018: International Journal of Experimental Pathology
Sonja Šelemetjev, Aleksandar Bartolome, Tijana Išić Denčić, Ilona Đorić, Ivan Paunović, Svetislav Tatić, Dubravka Cvejić
Epidermal growth factor receptor (EGFR) and its downstream effector, focal adhesion kinase (FAK), have been shown to be overexpressed frequently in human malignancies and implicated in tumour aggressiveness. We aimed to investigate the relationship between EGFR and FAK expression and their possible correlation with the clinical phenotype of patients with papillary thyroid carcinoma (PTC). Expression profiles of EGFR and FAK were analysed in PTC tissue samples (n = 104) by immunohistochemistry and Western blotting...
April 2018: International Journal of Experimental Pathology
Alaknanda Mishra, Shailendra Arindkar, Preeti Sahay, Jerald Mahesh Kumar, Pramod K Upadhyay, Subeer S Majumdar, Perumal Nagarajan
Non-alcoholic fatty liver disease (NAFLD)-like conditions enhance the production and action of clotting factors in humans. However, studies examining the effect of NAFLD due to high-fat high-fructose (HFHF) diet in factor VIII-deficient (haemophilia A) animals or patients have not been reported previously. In this study, we investigated the individual role of factor VIII in the progression of diet-induced NAFLD in the factor 8-/- (F8-/- ) mouse model system and its consequences on the haemophilic status of the mice...
February 2018: International Journal of Experimental Pathology
Wen-Wu Dong, Jian Li, Jing Li, Ping Zhang, Zhi-Hong Wang, Wei Sun, Hao Zhang
Oestrogens play an important role in the development and progression of papillary thyroid carcinoma (PTC) through oestrogen receptor (ER)-α and -β, which may exert different or even opposing actions in PTC. The roles of ERβ in ERα-negative PTC are still not clear. This study investigated the expression dynamics of ERβ1 (wild-type ERβ) and its clinical significance in female ERα-negative PTC patients. ERβ1 expression was detected in thyroid tissues of 136 female patients diagnosed with PTC. The relationships between ERβ1 expression and clinicopathological/biological factors were also analysed in female ERα-negative PTC patients...
February 2018: International Journal of Experimental Pathology
Xiaowen Cheng, Martina Svensson, Yiyi Yang, Tomas Deierborg, Eva Ekblad, Ulrikke Voss
Reduced blood flow to the brain induces cerebral ischaemia, potentially causing central injury and peripheral complications including gastrointestinal (GI) dysfunction. The pathophysiology behind GI symptoms is suspected to be neuropathy in the enteric nervous system (ENS), which is essential in regulating GI function. This study investigates if enteric neuropathy occurs after cerebral ischaemia, by analysing neuronal survival and relative numbers of vasoactive intestinal peptide (VIP) and neuronal nitric oxide synthase (nNOS) expressing neurons in mouse ileum after three types of cerebral ischaemia...
February 2018: International Journal of Experimental Pathology
Elena Rainero
Integrins are the key mediators of cell-extracellular matrix (ECM) interaction, linking the ECM to the actin cytoskeleton. Besides localizing at the cell surface, they can be internalized and transported back to the plasma membrane (recycled) or delivered to the late endosomes/lysosomes for degradation. We and others have shown that integrin can be endocytosed together with their ECM ligands. In this short review, I will highlight how extracellular protein (including ECM) endocytosis impinges on the activation of the mechanistic target of rapamycin (mTOR) pathway, a master regulator of cell metabolism and growth...
February 2018: International Journal of Experimental Pathology
Andrieli T Hauschildt, Luciana A Corá, Gustavo T Volpato, Yuri K Sinzato, Débora C Damasceno, Madileine F Américo
Moderate hyperglycaemic levels seem to be related to abnormal gastric motility in diabetes mellitus. However, experimental models designed to evaluate the relationship between motility and diabetes over time are not yet well established. Our objective was to investigate the long-term effects of mild diabetes on gastric motility in rats. Newborn male rats received streptozotocin (mild diabetes groups - MD) or vehicle (control groups - C), and both groups were evaluated after 3 (C3 and MD3) and 6 months (C6 and MD6) postinduction...
February 2018: International Journal of Experimental Pathology
Ahmed A M Abdel-Hamid, Hoda Atef, Khaled R Zalata, Atef Abdel-Latif
Although there is emerging evidence that mast cells are involved in infertility, their exact role has not been elucidated clearly. Here we carried out a retrospective case-control study to find out whether there is a correlation between mast cell (MC) count and proliferation (Ki67 index) of the spermatogenic epithelium as well as of the Sertoli cells (vimentin-positive) in non-obstructive azoospermia (NOA). We assessed MCs, Ki67 and vimentin expression in Sertoli cells in testicular biopsies of germ cell aplasia (GCA, n = 14) and maturation arrest (MA, n = 14) vs...
February 2018: International Journal of Experimental Pathology
Ignazio Puccio, Saif Khan, Adil Butt, David Graham, Vinay Sehgal, Dominic Patel, Marco Novelli, Laurence B Lovat, Manuel Rodriguez-Justo, Rifat A Hamoudi
Non-dysplastic Barrett's oesophagus (NDBE) occurs as a consequence of an inflammatory response triggered through prolonged gastro-oesophageal reflux and it may precede the development of oesophageal adenocarcinoma. NF-κB activation as a result of the inflammatory response has been shown in NDBE, but the possible mechanism involved in the process is unknown. The aim of this study was to assess, using immunohistochemistry, Survivin and Bcl3 expression as potential biomarkers for NF-κB activation along the oesophageal metaplasia-dysplasia-adenocarcinoma sequence...
February 2018: International Journal of Experimental Pathology
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