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International Journal of Experimental Pathology

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https://www.readbyqxmd.com/read/28925080/specific-knock-down-of-tissue-non-specific-alkaline-phosphatase-mrna-levels-inhibits-intracellular-lipid-accumulation-in-3t3-l1-and-hepg2-cells
#1
George Chirambo, Chantal van Niekerk, Nigel J Crowther
The use of non-specific inhibitors of tissue non-specific alkaline phosphatase (TNSALP) in pre-adipocytes blocks intracellular lipid accumulation. TNSALP is also expressed in hepatocytes, which are known to accumulate lipid in a similar manner to pre-adipocytes. The purpose of this study was to use specific silencing of TNSALP mRNA, using short interfering (si) RNA, to investigate the role of TNSALP in intracellular lipid accumulation in 3T3-L1 and HepG2 cells. Cellular activity of TNSALP was measured using an automated colorimetric assay, and intracellular lipid accumulation was determined using the lipid-specific dye, Oil Red O...
September 19, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28905442/fibrillin-microfibrils-structural-tensometers-of-elastic-tissues
#2
REVIEW
Cay M Kielty
Fibrillin microfibrils are indispensable structural elements of connective tissues in multicellular organisms from early metazoans to humans. They have an extensible periodic beaded organization, and support dynamic tissues such as ciliary zonules that suspend the lens. In tissues that express elastin, including blood vessels, skin and lungs, microfibrils support elastin deposition and shape the functional architecture of elastic fibres. The vital contribution of microfibrils to tissue form and function is underscored by the heritable fibrillinopathies, especially Marfan syndrome with severe elastic, ocular and skeletal tissue defects...
September 14, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28895242/hepatocellular-carcinoma-in-a-mouse-model-fed-a-choline-deficient-l-amino-acid-defined-high-fat-diet
#3
Ayae Ikawa-Yoshida, Saori Matsuo, Atsuhiko Kato, Yusuke Ohmori, Atsuko Higashida, Eiji Kaneko, Masahiko Matsumoto
Hepatocellular carcinoma (HCC) is a common cancer worldwide and represents the outcome of the natural history of chronic liver disease. The growing rates of HCC may be partially attributable to increased numbers of people with non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). However, details of the liver-specific molecular mechanisms responsible for the NAFLD-NASH-HCC progression remain unclear, and mouse models that can be used to explore the exact factors that influence the progression of NAFLD/NASH to the more chronic stages of liver disease and subsequent HCC are not yet fully established...
September 12, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28895246/influence-of-indigenous-microbiota-on-experimental-toxoplasmosis-in-conventional-and-germ-free-mice
#4
Bruna B Nascimento, Christiane T Cartelle, Maria de L Noviello, Breno V Pinheiro, Ricardo W de Almeida Vitor, Danielle da G Souza, Simone de Vasconcelos Generoso, Valbert N Cardoso, Flaviano Dos S Martins, Jacques R Nicoli, Rosa M E Arantes
Toxoplasmosis represents one of the most common zoonosis worldwide. Its agent, Toxoplasma gondii, causes a severe innate pro-inflammatory response. The indigenous intestinal microbiota promotes host animal homoeostasis and may protect the host against pathogens. Germ-free (GF) animals provide an important tool for the study of interactions between host and microbiota. In this study, we assessed the role of indigenous microorganisms in disease development utilizing a murine toxoplasmosis model, which includes conventional (CV) and GF NIH Swiss mice...
September 11, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28884865/physical-training-improves-visceral-adipose-tissue-health-by-remodelling-extracellular-matrix-in-rats-with-estrogen-absence-a-gene-expression-analysis
#5
Fernanda O Duarte, Camila do Valle Gomes-Gatto, Jorge C Oishi, Anderson Diogo de S Lino, Uliana S Stotzer, Maria Fernanda C Rodrigues, Guilherme H Gatti da Silva, Heloisa S Selistre-de-Araújo
Adipose tissue development is associated with modifications involving extracellular matrix remodelling, and metalloproteinases play a significant role in this process. Reduced circulating sexual hormones cause impacts on the size, morphology and functions of the adipose tissue, increasing susceptibility to diseases. This study investigated whether exercise training may be an alternative strategy to combat the effects promoted by estrogen decay through modulation in gene expression patterns in the extracellular matrix (ECM) of visceral adipose tissue of ovariectomized rats...
September 8, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28849621/histological-morphometric-protein-and-gene-expression-analyses-of-rat-retinas-with-ischaemia-reperfusion-injury-model-treated-with-sildenafil-citrate
#6
Diogo S Zanoni, Germana A Da Silva, Raaya Ezra-Elia, Márcio Carvalho, Juliany G Quitzan, Ron Ofri, José L Laus, Renee Laufer-Amorim
The aim of this study was to better understand the role of apoptosis in a retinal ischaemia-reperfusion injury model and to determine whether sildenafil citrate treatment can prevent retinal cell apoptosis. Thirty-six rats were divided into a control group (n = 6) and two experimentally induced ischaemia-reperfusion groups (7 and 21 days; n = 15 per group). The induced ischaemia-reperfusion groups were treated with sildenafil for 7 and 21 days (n = 10 per group), and 10 animals were treated with a placebo for the same period (n = 5 per group)...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28849620/abstracts
#7
(no author information available yet)
No abstract text is available yet for this article.
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28749083/evidence-of-bone-marrow-downregulation-in-brain-dead-rats
#8
Laura Menegat, Rafael Simas, Julia M Caliman, Fernando L Zanoni, Jacqueline F Jacysyn, Luiz Fernando F da Silva, Primavera Borelli, Luiz Felipe P Moreira, Paulina Sannomiya
Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis [annexin V/propidium iodide (PI) protocol]...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28664583/corticosterone-influences-gerbil-meriones-unguiculatus-prostatic-morphophysiology-and-alters-its-proliferation-and-apoptosis-rates
#9
Julia Quilles Antoniassi, Ricardo Alexandre Fochi, Rejane Maira Góes, Patricia Simone Leite Vilamaior, Sebastião Roberto Taboga
Glucocorticoids (GCs) are hormones that are widely used in medicine; but although side effects are generally recognised, little is known about the precise mechanisms that is implicated in many of these side effects. Furthermore, GCs are highly correlated with stress and behaviour disorders. This study evaluated the effects of the glucocorticoid corticosterone on the ventral prostate of the Mongolian gerbil. Male gerbils (Meriones unguiculatus) (n = 5) received intraperitoneal injections of saline or corticosterone in doses of 0...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28621016/acute-effects-of-phenylbutyrate-on-glutamine-branched-chain-amino-acid-and-protein-metabolism-in-skeletal-muscles-of-rats
#10
Milan Holecek, Melita Vodenicarovova, Pavel Siman
Phenylbutyrate (PB) acts as chemical chaperone and histone deacetylase inhibitor, which is used to decrease ammonia in urea cycle disorders and has been investigated for use in the treatment of a number of lethal illnesses. We performed in vivo and in vitro experiments to examine the effects of PB on glutamine (GLN), branched-chain amino acid (BCAA; valine, leucine and isoleucine) and protein metabolism in rats. In the first study, animals were sacrificed one hour after three injections of PB (300mg/kg b...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28585282/models-to-define-the-stages-of-articular-cartilage-degradation-in-osteoarthritis-development
#11
REVIEW
Blandine Poulet
Osteoarthritis (OA) is a common chronic disorder that affects an increasing number of the ageing population. Despite the prevalence, there are currently no therapies. Defining new therapies that target specific pathogenic phases of disease development relies on the effective separation of the different stages of OA. This manuscript reviews the tissues and models that are being used to separate these stages of disease, in particular initiation and early and late progression. These models include human tissues with known initiating factors, the use of anatomical locations with defined relationships to the primary cartilage lesion area, timing of OA development in well-described animal models and the versatility of a non-invasive model of murine knee joint trauma...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28581201/grape-juice-concentrate-alleviates-epididymis-and-sperm-damage-in-cadmium-intoxicated-rats
#12
Celina de A Lamas, Livia Cuquetto-Leite, Emanueli do Nascimento da Silva, Bruna F Thomazini, Gabriel da S Cordeiro, Fabrícia de S Predes, Andrea P B Gollücke, Heidi Dolder
The possibility of long-term grape juice concentrate (GJC) consumption conferring a protective effect against cadmium (Cd)-induced damage to the epididymis, completely preserving sperm profile, was evaluated here for the first time in the scientific literature. Male Wistar rats (n = 6/per group) received an intraperitoneal Cd injection (1.2 mg/Kg) at age 80 days and GJC (2 g/Kg) by gavage from 50 days until 136 days old. Groups receiving either Cd or GJC were added. An intraperitoneal injection of saline (0...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28580701/identification-of-dj-1-as-a-contributor-to-multidrug-resistance-in-human-small-cell-lung-cancer-using-proteomic-analysis
#13
Hongyi Gao, Yuchun Niu, Man Li, Shun Fang, Linlang Guo
Proteomic approaches have been proven to provide an important tool in identifying drug resistance-associated proteins. The aim of this study was to investigate the protein profiling of drug resistance-related proteins in small-cell lung cancer (SCLC) by proteomic analysis. The proteomic profiling was performed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF-TOF of SCLC in the multidrug-resistant cell line H69AR and its parental cell line H69. A total of 11 proteins were identified to be >2-fold up-or downregulated between the two cell lines...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28556971/low-level-laser-therapy-780%C3%A2-nm-combined-with-collagen-sponge-scaffold-promotes-repair-of-rat-cranial-critical-size-defects-and-increases-tgf-%C3%AE-fgf-2-opg-rank-and-osteocalcin-expression
#14
Lana Sarita de Souza de Oliveira, Aurigena Antunes de Araújo, Raimundo Fernandes de Araújo Júnior, Carlos Augusto Galvão Barboza, Boniek Castillo Dutra Borges, José Sandro Pereira da Silva
The aim of this study was to evaluate the effect of collagen sponge scaffold (CSS) implantation associated with low-level laser therapy (LLLT) on repairing bone defects. A single 5-mm cranial defect was surgically created in forty Wistar rats, which then received one of the following four interventions (n = 10 per group): no treatment (G0); bone defect implanted with collagen sponge scaffold (CSS) alone (G1); defect treated with low-level laser therapy (LLLT) (wavelength 780 nm; total energy density 120 J/cm(2) ; power 50 mW) alone (G2); and CSS associated with LLLT treatment (G3)...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28543963/ageing-is-associated-with-brown-adipose-tissue-remodelling-and-loss-of-white-fat-browning-in-female-c57bl-6-mice
#15
Leidyanne Ferreira Gonçalves, Thaissa Queiroz Machado, Camila Castro-Pinheiro, Nathalia Guimaraes de Souza, Karen Jesus Oliveira, Caroline Fernandes-Santos
Fat storage changes throughout life and affects body metabolism. Ageing impact on brown (BAT) and white adipose tissue (WAT) deserves attention, especially in females, because they are less prone to age-induced weight gain. While in male mice the impact of ageing on adipose tissue remodelling is well characterized, the effects in female mice remain largely unclear. Thus, we investigated BAT and WAT remodelling during ageing in female C57BL/6 mice. At 3 months, body weight was 24 ± 0.3 g (mean±SD), and it increased from 6 to 9 months of age (+20%, P < 0...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28543723/neuromuscular-junctions-nmjs-ultrastructural-analysis-and-nicotinic-acetylcholine-receptor-nachr-subunit-mrna-expression-in-offspring-subjected-to-protein-restriction-throughout-pregnancy
#16
Paula Aiello Tomé de Souza Castro, Ludimila Canuto Faccioni, Patrícia Aline Boer, Robson Francisco Carvalho, Selma Maria Michelin Matheus, Maeli Dal-Pai-Silva
Protein restriction during gestation can alter the skeletal muscle phenotype of offspring; however, little is known with regard to whether this also affects the neuromuscular junction (NMJ), as muscle phenotype maintenance depends upon NMJ functional integrity. This study aimed to evaluate the effects of a low protein (6%) intake by dams throughout gestation on male offspring NMJ morphology and nicotinic acetylcholine receptor (nAChR) α1, γ and ε subunit expression in the soleus (SOL) and extensor digitorum longus (EDL) muscles...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28439920/understanding-human-thiol-dioxygenase-enzymes-structure-to-function-and-biology-to-pathology
#17
REVIEW
Bibekananda Sarkar, Mahesh Kulharia, Anil K Mantha
Amino acid metabolism is a significant metabolic activity in humans, especially of sulphur-containing amino acids, methionine and cysteine (Cys). Cys is cytotoxic and neurotoxic in nature; hence, mammalian cells maintain a constant intracellular level of Cys. Metabolism of Cys is mainly regulated by two thiol dioxygenases: cysteine dioxygenase (CDO) and 2-aminoethanethiol dioxygenase (ADO). CDO and ADO are the only human thiol dioxygenases reported with a role in Cys metabolism and localized to mitochondria...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28556395/effects-of-exercise-training-on-breast-cancer-metastasis-in-a-rat-model
#18
Antonieta Alvarado, Rui M Gil da Costa, Ana I Faustino-Rocha, Rita Ferreira, Carlos Lopes, Paula A Oliveira, Bruno Colaço
Exercise training is thought to play a protective role against cancer development and metastasis, either by reducing hormonal stimulation of hormone-dependent cancers or by reducing the permeability of vascular walls towards invading metastatic cells. The purpose of this work was to evaluate the role of long-term exercise training in the development and metastasis of breast cancer, in an immune-competent 1-methyl-1-nitrosourea (MNU) induced rat model. A single MNU dose was administered to Sprague-Dawley rats at 50 days of age and the rats were subjected to exercise training on a treadmill at 20 m/min, 60 min/day, 5 days/week for 35 weeks...
February 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28508516/fell-muir-lecture-collagen-fibril-formation-in-vitro-and-in-vivo
#19
REVIEW
Karl E Kadler
It is a great honour to be awarded the Fell Muir Prize for 2016 by the British Society of Matrix Biology. As recipient of the prize, I am taking the opportunity to write a minireview on collagen fibrillogenesis, which has been the focus of my research for 33 years. This is the process by which triple helical collagen molecules assemble into centimetre-long fibrils in the extracellular matrix of animals. The fibrils appeared a billion years ago at the dawn of multicellular animal life as the primary scaffold for tissue morphogenesis...
February 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28449369/exposure-to-the-insecticide-endosulfan-induces-liver-morphology-alterations-and-oxidative-stress-in-fruit-eating-bats-artibeus-lituratus
#20
Jerusa Maria Oliveira, Alessandro Brinati, Liany Divina Lima Miranda, Danielle Barbosa Morais, José Cola Zanuncio, Reggiani Vilela Gonçalves, Maria do Carmo Gouveia Peluzio, Mariella Bontempo Freitas
Exposure to pesticides may increase the generation of reactive oxygen species (ROS), leading to oxidation of cell membrane lipids and proteins. Although fruit bats are potentially exposed to pesticides during their entire lifespan, the impacts of this exposure are still poorly investigated. We examined the effects of low, commercially recommended concentrations (0, 1.05 and 2.1 g/l) of an organochlorine insecticide endosulfan (EDS) formulation on oxidative responses in the liver and kidneys of Neotropical fruit bats (Artibeus lituratus), as well as possible liver morphological alterations following a 35-day oral exposure...
February 2017: International Journal of Experimental Pathology
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