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World Journal of Microbiology & Biotechnology

Jun-Nan Zhang, Fang Zhang, Qing-Juan Tang, Chuan-Shan Xu, Xiang-Hong Meng
The present study has focused on the effects of hypericin (Hyp) based photodynamic inactivation (PDI) of Escherichia coli (E. coli). To evaluate the efficiency of Hyp based PDI of E. coli, single factor experiments and response surface optimization experiment were conducted to obtain the optimum parameter values (36 µM Hyp, 5.9 J cm-2 light dose: 16.4 mW cm-2 , 60 W, 260 s, 590 nm and 68 min incubation time) and finally achieved a 4.1 log CFU mL-1 decrease of E. coli. Cell-Hyp interaction and intracellular reactive oxygen species (ROS) level were detected by fluorescence spectrometric photometer...
June 20, 2018: World Journal of Microbiology & Biotechnology
Sana Tounsi, Kaouthar Feki, Mohamed Najib Saïdi, Sahar Maghrebi, Faiçal Brini, Khaled Masmoudi
HKT transporters which mediate Na+ -specific transport or Na+ -K+ co-transport, play an important role in protecting plants from salinity stress by preventing Na+ -over-accumulation in leaves. In this work, a 1508-bp genomic fragment upstream of the TmHKT1;4-A1 translated sequence from Triticum monococcum has been isolated, cloned, and designated as the ''PrTmHKT1;4-A1'' promoter. Sequence analysis of ''PrTmHKT1;4-A1'' revealed the presence of cis-regulatory elements which could be required for abiotic stress and abscisic acid (ABA) responsiveness...
June 20, 2018: World Journal of Microbiology & Biotechnology
Tsvetelina Yungareva, Zoltan Urshev
In lactobacilli the aggregation phenotype is linked to their ability to colonize the intestinal and urogenital tracts and to counteract pathogenic bacteria. In all available complete genome sequences of Lactobacillus delbrueckii ssp. bulgaricus there are at least two genes putatively related to aggregation, one of which is annotated as aggregation-promoting factor (apf). Here we report the results from the in silico analysis of this gene and its product. The apf gene was present in the genome of all 70 tested L...
June 19, 2018: World Journal of Microbiology & Biotechnology
Ines Touati, Nicolas Ruiz, Olivier Thomas, Irina S Druzhinina, Lea Atanasova, Olfa Tabbene, Salem Elkahoui, Roudaina Benzekri, Lamjed Bouslama, Yves François Pouchus, Ferid Limam
A Trichoderma orientale strain LSBA1 was isolated from the Mediterranean marine sponge Cymbaxinella damicornis. The crude extract of T. orientale mycelium showed inhibitory activity against growth of Gram-positive and Gram-negative bacteria as well as clinical isolates of Candida albicans. Purification of the anti-Candida component was performed using a combination of open silica gel-60 column and reverse phase high performance liquid chromatography. The active compound called hyporientalin A has been identified as a peptaibol analogue of longibrachin-A-II using mass spectrometry...
June 19, 2018: World Journal of Microbiology & Biotechnology
Changsu Lee, Joon-Woo Ahn, Jin-Baek Kim, Jee Young Kim, Yoon-E Choi
The unicellular green microalga Haematococcus pluvialis has the highest content of the natural antioxidant, astaxanthin. Previously, it was determined that astaxanthin accumulation in H. pluvialis could be induced by blue-wavelength irradiation; however, the molecular mechanism remains unknown. The present study aimed to compare the transcriptome of H. pluvialis, with respect to astaxanthin biosynthesis, under the monochromatic red (660 nm) or blue (450 nm) light-emitting diode (LED) irradiation. Among a total of 165,372 transcripts, we identified 67,703 unigenes, of which 2245 and 171 were identified as differentially expressed genes (DEGs) in response to blue and red irradiation, respectively...
June 18, 2018: World Journal of Microbiology & Biotechnology
Arzu Coleri Cihan, Emine Derebay Yildiz, Ergin Sahin, Ozal Mutlu
Among the thermophilic Bacillaceae family members, α-amylase production of 15 bacilli from genus Anoxybacillus was investigated, some of which are biotechnologically important. These Anoxybacillus α-amylase genes displayed ≥ 91.0% sequence similarities to Anoxybacillus enzymes (ASKA, ADTA and GSX-BL), but relatively lower similarities to Geobacillus (≤ 69.4% to GTA, Gt-amyII), and Bacillus aquimaris (≤ 61.3% to BaqA) amylases, all formerly proposed only in a Glycoside Hydrolase 13 (GH13) subfamily...
June 15, 2018: World Journal of Microbiology & Biotechnology
Ralf Lopes, Sarina Tsui, Priscila J R O Gonçalves, Marisa Vieira de Queiroz
One of the major challenges of agriculture currently is to obtain higher crop yield. Environmental conditions, cultivar quality, and plant diseases greatly affect plant productivity. On the other hand, several endophytic Bacillus species have emerged as a complementary, efficient, and safe alternative to current crop management practices. The ability of Bacillus species to form spores, which resist adverse conditions, is an advantage of the genus for use in formulations. Endophytic Bacillus species provide plants with a wide range of benefits, including protection against phytopathogenic microorganisms, insects, and nematodes, eliciting resistance, and promoting plant growth, without causing damage to the environment...
June 13, 2018: World Journal of Microbiology & Biotechnology
Eduardo M Costa, Sara Silva, Mariana Veiga, Freni K Tavaria, Maria M Pintado
Over the years the body of work relating chitosan and its possible skin-related applications has grown, showing that chitosan is active both as a free compound and as a functional finishing of textiles. As a free molecule chitosan proved itself to be an attractive option as it is biocompatible and has a demonstrated biological activity (e.g. growth inhibition or adhesion inhibition) upon multiple skin pathogens, even upon multidrug resistant species. Furthermore, chitosan has wound healing accelerating properties, which make it a prime candidate for possible control of skin related infections...
June 13, 2018: World Journal of Microbiology & Biotechnology
Xiaoxiao Lu, Yongzhi Liu, Linchao Zhao, Yongnan Liu, Mingwen Zhao
This study aimed to identify suitable reference genes under three chemical inducers, methyl jasmonate (MeJA), salicylic acid (SA) and hydrogen peroxide (H2 O2 ) in Ganoderma lucidum. In this study, expression stabilities of 14 candidate reference genes had been validated. Four algorithms were used: geNorm, NormFinder, BestKeeper, and RefFinder. Our results showed that, in short time, UCE2 (ubiquitin conjugating enzyme) was the most stable gene both in MeJA and H2 O2 treatments, ACTIN (beta-actin) was the most suitable reference gene for SA treatment...
June 12, 2018: World Journal of Microbiology & Biotechnology
Siegfried Brunner, Erik Eppinger, Stefanie Fischer, Janosch Gröning, Andreas Stolz
The conversion of aliphatic nitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191 (NitA) was analyzed. The nitrilase hydrolysed a wide range of aliphatic mono- and dinitriles and showed a preference for unsaturated aliphatic substrates containing 5-6 carbon atoms. In addition, increased reaction rates were also found for aliphatic nitriles carrying electron withdrawing substituents (e.g. chloro- or hydroxy-groups) close to the nitrile group. Aliphatic dinitriles were attacked only at one of the nitrile groups and with most of the tested dinitriles the monocarboxylates were detected as major products...
June 12, 2018: World Journal of Microbiology & Biotechnology
D D Barbosa, S L Brito, P D Fernandes, P I Fernandes-Júnior, L M Lima
Drought is one of the environmental factors that most affects peanut cultivation in semi-arid regions, resulting in economic losses to growers. However, growth promoting bacteria are able to reduce water deficit damage in some plant species. In this context, this study aimed to evaluate the interaction of Bradyrhizobium strains reducing water stress effects on peanut genotypes by antioxidant enzymes activities, leaf gas exchanges and vegetative growth, as well as to determine the taxonomic positioning of strain ESA 123...
June 9, 2018: World Journal of Microbiology & Biotechnology
Xiao-Yong Zhang, Wen Fu, Xiao Chen, Mu-Ting Yan, Xian-De Huang, Jie Bao
To search for more microbial resources for screening environment-friendly antifoulants, we investigated the phylogenetic diversity and antifouling potentials of culturable fungi in mangrove sediments from Techeng Isle, China. A total of 176 isolates belonging to 57 fungal taxa were recovered and identified. The high levels of diversity and abundance of mangrove fungi from Techeng Isle were in accordance with previous studies on fungi from other mangrove ecosystems. Fifteen of the 176 isolates demonstrated high divergence (87-93%) from the known fungal taxa in GenBank...
June 9, 2018: World Journal of Microbiology & Biotechnology
Jian Yu
With rapid technology progress and cost reduction, clean hydrogen from water electrolysis driven by renewable powers becomes a potential feedstock for CO2 fixation by hydrogen-oxidizing bacteria. Cupriavidus necator (formally Ralstonia eutropha), a representative member of the lithoautotrophic prokaryotes, is a promising producer of polyhydroxyalkanoates and single cell proteins. This paper reviews the fundamental properties of the hydrogen-oxidizing bacterium, the metabolic activities under limitation of individual gases and nutrients, and the value-added products from CO2 , including the products with large potential markets...
June 9, 2018: World Journal of Microbiology & Biotechnology
Joseph Guevara-Luna, Patricia Alvarez-Fitz, Elvira Ríos-Leal, Macdiel Acevedo-Quiroz, Sergio Encarnación-Guevara, Ma Elena Moreno-Godinez, Mildred Castellanos-Escamilla, Jeiry Toribio-Jiménez, Yanet Romero-Ramírez
Benzo[a]pyrene (BaP) is recognized as a potentially carcinogenic and mutagenic hydrocarbon, and thus, its removal from the environment is a priority. The use of thermophilic bacteria capable of biodegrading or biotransforming this compound to less toxic forms has been explored in recent decades, since it provides advantages compared to mesophilic organisms. This study assessed the biotransformation of BaP by the thermophilic bacterium Bacillus licheniformis M2-7. Our analysis of the biotransformation process mediated by strain M2-7 on BaP shows that it begins during the first 3 h of culture...
June 9, 2018: World Journal of Microbiology & Biotechnology
Andreia R Bucci, Larissa Marcelino, Renata K Mendes, Augusto Etchegaray
The oral pathogen Streptococcus mutans is involved in tooth decay by a process that initiates with biofilm adhesion and caries development. The presence of other microbes such as Candida albicans may worsen the demineralization process. Since both microbes are virulent to the host and will proliferate under specific host immune deficiencies and systemic diseases, it is important to study antimicrobial substances and their effects on both pathogens. There are several antiseptic agents used to reduce plaque biofilm and its outcome (dental caries and/or periodontal disease)...
June 6, 2018: World Journal of Microbiology & Biotechnology
Tiwa Rotchanapreeda, Wasinee Ngonsawan, Manadsaree Klomtun, Jamorn Somana
For decades, diverse plasmid vectors have been continuously developed for molecular cloning of DNA fragment in the bacterial host cell Escherichia coli. Even with deliberate performances in vector preparation, the cloning approaches still face inevitable background colonies, or false positive clones, that may be arisen from intact or self-ligated plasmid molecules. To assist in such problem, two plasmids, pBS2ndd and pBS3ndd, which resistant to ampicillin and kanamycin respectively, were developed in this study as more advantageous cloning vector...
June 6, 2018: World Journal of Microbiology & Biotechnology
Daniela Toma de Moraes Akamine, Daniel de Almeida Cozendey da Silva, Gabriela de Lima Câmara, Thayane Vieira Carvalho, Michel Brienzo
Cellulolytic enzymes have been studied in several organisms, such as insects, molluscs and other organisms, which can have enzymes endogenously produced or by symbiotic microorganisms. These enzymes are responsible for breaking down the cellulosic material upon which these organisms feed, probably with the aim of assimilating the sugars and nutrients. As Teredinidae bivalves grown in mangrove trees, this study aimed to measure endo-β-1,4-glucanase activity in different organs and its content. Endo-β-1,4-glucanase activity was detected in different organs of the Teredinidae bivalves, including gills and digestive organs tissues and its content...
June 1, 2018: World Journal of Microbiology & Biotechnology
Manju Suresh, N Nithya, P R Jayasree, K P Vimal, P R Manish Kumar
The present study deals with membrane-bound efflux pumps, MexAB-OprM and MexXY and their respective regulatory genes mexR, nalC, nalD and mexZ in multidrug resistant (MDR) Pseudomonas aeruginosa. Following antibiotic sensitivity testing and detection of various beta-lactamases, hyperexpression of efflux pump genes, mexB and mexY in the isolates was investigated using semi-quantitative and real-time reverse transcription-PCR. Amplicons from regulatory genes were sequenced and subjected to mutational and phylogenetic analysis...
May 30, 2018: World Journal of Microbiology & Biotechnology
Wenyuan Shi, Lanhua Zhao, Shengtao Li, Guizhen Xu, Yanhua Zeng
Nowadays, there is lack of effective serological detection method for Mycoplasma pneumoniae (M. pneumoniae) infection in clinic. In this study, the mimic epitopes of M. pneumoniae were screened to evaluate the role in the serodiagnosis of M. pneumoniae infection. The M. pneumoniae-positive serum was used as the target for biopanning to phage display random 7-peptide library. The positive phage clones were selected and the DNA were sequenced and analyzed by BLAST. The representative phages were identified using dot immunoblotting and ELISA...
May 29, 2018: World Journal of Microbiology & Biotechnology
Pamela Romina Bernabeu, Sabrina Soledad García, Ana Clara López, Santiago Adolfo Vio, Natalia Carrasco, José Luis Boiardi, María Flavia Luna
Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of P. tropica as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with P...
May 25, 2018: World Journal of Microbiology & Biotechnology
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