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World Journal of Microbiology & Biotechnology

Érica Barbosa Felestrino, Izadora Tabuso Vieira, Washington Luiz Caneschi, Isabella Ferreira Cordeiro, Renata de Almeida Barbosa Assis, Camila Gracyelle de Carvalho Lemes, Natasha Peixoto Fonseca, Angélica Bianchini Sanchez, Juan Carlos Caicedo Cepeda, Jesus Aparecido Ferro, Camila Carrião Machado Garcia, Flávio Fonseca do Carmo, Luciana Hiromi Yoshino Kamino, Leandro Marcio Moreira
Microorganisms associated with plants have a great biotechnological potential, but investigations of these microorganisms associated with native plants in peculiar environments has been incipient. The objective of this study was to analyze the plant growth-promoting bacteria potential of cultivable bacteria associated with rare plants from the ferruginous rocky fields of the Brazilian Iron Quadrangle. The roots and rhizospheres of nine endemic plants species and samples of a root found in a lateritiric duricrust (canga) cave were collected, the culturable bacteria isolated and prospected for distinct biotechnological and ecological potentials...
October 4, 2018: World Journal of Microbiology & Biotechnology
Ting Xue, Kui Liu, Duo Chen, Xue Yuan, Jingping Fang, Hansong Yan, Luqiang Huang, Youqiang Chen, Wenjin He
Bioethanol, as a form of renewable and clean energy, has become increasingly important to the energy supply. One major obstacle in ethanol production is developing a high-capacity system. Existing approaches for regulating the ethanol production pathway are relatively insufficient, with nonspecific genetic manipulation. Here, we used CRISPR/Cas9 technology to disrupt the alcohol dehydrogenase (ADH) 2 gene via complete deletion of the gene and introduction of a frameshift mutation in the ADH2 locus. Sequencing demonstrated the accurate knockout of the target gene with 91...
October 1, 2018: World Journal of Microbiology & Biotechnology
Yanfen Cheng, Qicheng Shi, Ruolin Sun, Dong Liang, Yuanfei Li, Yuqi Li, Wei Jin, Weiyun Zhu
Anaerobic fungi (phylum Neocallimastigomycota), an early branching family of fungi, are commonly encountered in the digestive tract of mammalian herbivores. To date, isolates from ten described genera have been reported, and several novel taxonomic groupings are detected using culture-independent molecular methods. Anaerobic fungi are recognized as playing key roles in the decomposition of lignocellulose (up to 50% of the ingested and untreated lignocellulose), with their physical penetration and extracellular enzymatical secretion of an unbiased diverse repertoire of cell-wall-degrading enzymes...
October 1, 2018: World Journal of Microbiology & Biotechnology
Kun-Qiang Hong, Ding-Yu Liu, Tao Chen, Zhi-Wen Wang
Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR) system, which uses an easily customizable specificity RNA-guided DNA endonuclease, such as Cas9. Recently, CRISPR/Cas9 mediated genome engineering has been widely applied to model organisms, including Bacillus subtilis, enabling facile, rapid high-fidelity modification of endogenous native genes...
September 29, 2018: World Journal of Microbiology & Biotechnology
Kelly Cristina Dos Reis, Javier Arrizon, Lorena Amaya-Delgado, Anne Gschaedler, Rosane Freitas Schwan, Cristina Ferreira Silva
Vinasse is a waste obtained from the production of beverages, such as tequila and cachaça. The presence of acids, alcohols, sugars, minerals, amino acids, peptides, and nitrogen salts make vinasse a hazardous liquid waste to the environment, affecting the fauna, flora, and microbiota of rivers and lagoons. This study used biological treatment concomitant to volatile compound production. The yeasts used in the study were Saccharomyces cerevisiae (CCMA 0187 and CCMA 0188), Candida parapsilosis (CCMA 0544), and Pichia anomala (CCMA 0193)...
September 28, 2018: World Journal of Microbiology & Biotechnology
Makoto Yoshimoto, Peter Walde
Carbonic anhydrase (CA) is an essential metalloenzyme in living systems for accelerating the hydration and dehydration of carbon dioxide. CA-catalyzed reactions can be applied in vitro for capturing industrially emitted gaseous carbon dioxide in aqueous solutions. To facilitate this type of practical application, the immobilization of CA on or inside solid or soft support materials is of great importance because the immobilization of enzymes in general offers the opportunity for enzyme recycling or long-term use in bioreactors...
September 26, 2018: World Journal of Microbiology & Biotechnology
Somesh Singh, Krunal A Patel, Prashant D Sonawane, Rishi K Vishwakarma, Bashir M Khan
This work used an approach of enzyme engineering towards the improved production of baicalin as well as alteration of acceptor and donor substrate preferences in UGT73A16. The 3D model of Withania somnifera family-1 glycosyltransferase (UGT73A16) was constructed based on the known crystal structures of plant UGTs. Structural and functional properties of UGT73A16 were investigated using docking and mutagenesis. The docking studies were performed to understand the key residues involved in substrate recognition...
September 25, 2018: World Journal of Microbiology & Biotechnology
Lijun Wang, Ronghui Wang, Hua Wei, Yanbin Li
Aptamers are short nucleotide sequences which can specifically bind to a variety of targets with high affinity. They are identified and selected via systematic evolution of ligands by exponential enrichment (SELEX). Compared to antibodies, aptamers offer several advantages including easy labeling, high stability and lower cost. Those advantages make it possible to be a potential for use as a recognition probe to replace antibody in the diagnostic field. This article is intended to provide a comprehensive review, which is focused on systemizing recent advancements concerning SELEX procedures, with special emphasis on the key steps in SELEX procedures...
September 15, 2018: World Journal of Microbiology & Biotechnology
Shouyun Lv, Xin Chen, Chunye Mou, Shenghong Dai, Yinbing Bian, Heng Kang
Morchella importuna is a worldwide distributed edible mushroom with high ecological and economic values, but the molecular and genetic research about this mushroom has been hindered due to lack of an efficient transformation method. Here, we report for the first time the successful transformation of M. importuna by using a hypervirulent Agrobacterium tumefaciens strain bearing the constructed binary plasmid p1391-U-GUS. The selectable markers used were the genes for hygromycin resistance under the control of the polyubquitin promoter from M...
September 14, 2018: World Journal of Microbiology & Biotechnology
Bonnie A McNeil, David T Stuart
Oils and oleochemicals produced by microbial cells offer an attractive alternative to petroleum and food-crop derived oils for the production of transport fuel and oleochemicals. An emerging candidate for industrial single cell oil production is the oleaginous yeast Lipomyces starkeyi. This yeast is capable of accumulating storage lipids to concentrations greater than 60% of the dry cell weight. From the perspective of industrial biotechnology L. starkeyi is an excellent chassis for single-cell oil and oleochemical production as it can use a wide variety of carbon and nitrogen sources as feedstock...
September 12, 2018: World Journal of Microbiology & Biotechnology
Ming Li, Hong-Wei Hu, Ze Chen, Ya-Xian Zhang, Hao Li
Bioethanol fermentation is usually contaminated by bacteria, especially lactic acid bacteria (LAB), thereby leading to decrease of bioethanol yield and serious economic losses. Nisin is safer for controlling of bacterial contamination than antibiotics that are widely applied in industry. Moreover, in LAB contaminative bioethanol fermentation system, consistently decreased pH value provides opportunity to realize pH value responsive material-based release of anti-bacteria substances for intelligent and persistent controlling of bacterial contamination...
September 11, 2018: World Journal of Microbiology & Biotechnology
Elena R Ramos, Ricardo A Santos, Encarna Velázquez, Carmen E Velezmoro, Doris E Zúñiga
Fermentation microorganisms, lactic acid bacteria (LAB) and yeast from 12 samples of tunta production chain were quantified, from the native potatoes used by the process fermentation of potatoes in the river up to the final product. During fermentation, the LAB population steadily increased from 3 to 4 to 8 log CFU/g during the first 8 days in the river and the yeast population increased from 2 to 3 to 3-4 log CFU/g. Overall, 115 LAB strains were isolated using a culture-dependent method. Molecular techniques and 16S rRNA gene sequencing enabled the identification of native species...
September 10, 2018: World Journal of Microbiology & Biotechnology
Lei Han, Bo Liang
Nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH), as two kinds of well-known cofactor, are widely used in the most of enzymatic redox reactions, playing an important role in industrial catalysis. In general, supply of NAD(P)H is a major challenged factor in redox fermentation systems due to its high cost and low stability, which have stimulated the development of NADH regeneration systems in recent years. Until now, a series of NAD(P)H regeneration systems have been developed...
September 10, 2018: World Journal of Microbiology & Biotechnology
Pradeep Puligundla, Chulkyoon Mok
Bacterial and fungal spore contamination in different industries has a greater economic impact. Because of the remarkable resistance of spores to most physical and chemical microbicidal agents, their inactivation need special attention during sterilization processes. Heat and chemical sporicides are not always well suited for different sterilization/decontamination applications and carries inherent risks. In recent years, novel nonthermal agents including nonthermal plasmas are emerging as effective sporicides against a broad spectrum of bacterial and fungal spores...
September 10, 2018: World Journal of Microbiology & Biotechnology
Wenjing Cui, Laichuang Han, Feiya Suo, Zhongmei Liu, Li Zhou, Zhemin Zhou
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low %G+C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that displayed natural competence, this bacterium has been intensively considered to be an ideal model organism and a robust host to study several basic mechanisms, such as metabolism, gene regulation, bacterial differentiation, and application for industrial purposes, such as heterologous protein expression and the overproduction of an array of bioactive molecules...
September 10, 2018: World Journal of Microbiology & Biotechnology
Nallely Serrato-Gamiño, Ma Guadalupe Salgado-Lora, Martha P Chávez-Moctezuma, Jesús Campos-García, Carlos Cervantes
The Burkholderia xenovorans LB400 multireplicon genome displays a relatively high proportion of redundant genes, including several genes predicted to be related to arsenic resistance. These comprise an ars gene cluster, composed of the arsR3, acr3, arsC1 and arsH genes, as well as two arsB, arsC2, and seven individual arsR genes. The objective of this work was to elucidate the involvement of the ars gene cluster in arsenic resistance by the LB400 strain. Susceptibility tests showed that B. xenovorans LB400 is highly resistant to arsenate and arsenite...
September 10, 2018: World Journal of Microbiology & Biotechnology
Garima Anand, Rupam Kapoor
Alternaria leaf spot caused by Alternaria carthami is one of the most devastating diseases of safflower. Diversity among 95 isolates of A. carthami was determined using virulence assays, enzyme assays, dominant (ISSR) and co-dominant (SSR) markers. Collections and isolations were made from three major safflower producing states of India. The virulence assays categorised the population into four groups based on level of virulence. Estimation of activities of cell wall degrading enzymes (CWDE) yielded concurrent results to virulence assays with maximum CWDE activities in most virulent group...
August 31, 2018: World Journal of Microbiology & Biotechnology
Tatiana Kulakovskaya
The mechanisms of heavy metal resistance in microbial cells involve multiple pathways. They include the formation of complexes with specific proteins and other compounds, the excretion from the cells via plasma membrane transporters in case of procaryotes, and the compartmentalization of toxic ions in vacuoles, cell wall and other organelles in case of eukaryotes. The relationship between heavy metal tolerance and inorganic polyphosphate metabolism was demonstrated both in prokaryotic and eukaryotic microorganisms...
August 27, 2018: World Journal of Microbiology & Biotechnology
Sayak Mitra, Ashmita Das, Shampa Sen, Biswanath Mahanty
The widespread applications of silver nanoparticles in present days demand an industrial-scale production process. The ability of bacteria to synthesise silver nanoparticles can be exploited to overcome many shortcomings associated with conventional production processes, such as high cost and nanoparticle toxicity. However, lack of a standardised protocol and suboptimal yield remain a major obstacle for bacterial synthesis route. A potential, yet unexplored, solution to this problem could be envisioned through rewiring of the metabolic network to direct cellular resources towards the product of interest...
August 23, 2018: World Journal of Microbiology & Biotechnology
Jinjing Wang, Mengqi Li, Feiyun Zheng, Chengtuo Niu, Chunfeng Liu, Qi Li, Jinyuan Sun
Brewer's yeast is used in production of beer since millennia, and it is receiving increased attention because of its distinct fermentation ability and other biological properties. During fermentation, autolysis occurs naturally at the end of growth cycle of yeast. Yeast cell wall provides yeast with osmotic integrity and holds the cell shape upon the cell wall stresses. The cell wall of yeast consists of β-glucans, chitin, mannoproteins, and proteins that cross linked with glycans and a glycolipid anchor. The variation in composition and amount of cell wall polysaccharides during autolysis in response to cell wall stress, laying significant impacts on the autolysis ability of yeast, either benefiting or destroying the flavor of final products...
August 20, 2018: World Journal of Microbiology & Biotechnology
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