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World Journal of Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/28102516/production-of-lovastatin-and-itaconic-acid-by-aspergillus-terreus-a-comparative-perspective
#1
REVIEW
Tomasz Boruta, Marcin Bizukojc
Aspergillus terreus is a textbook example of an industrially relevant filamentous fungus. It is used for the biotechnological production of two valuable metabolites, namely itaconic acid and lovastatin. Itaconic acid serves as a precursor in polymer industry, whereas lovastatin found its place in the pharmaceutical market as a cholesterol-lowering statin drug and a precursor for semisynthetic statins. Interestingly, their biosynthetic gene clusters were shown to reside in the common genetic neighborhood. Despite the genomic proximity of the underlying biosynthetic genes, the production of lovastatin and itaconic acid was shown to be favored by different factors, especially with respect to pH values of the broth...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28074411/biomass-and-terpenoids-produced-by-mutant-strains-of-arthrospira-under-low-temperature-and-light-conditions
#2
Jian Guan, Songdong Shen, Hao Wu, Xin Liu, Weijie Shen, Yuan He, R Duan
The filamentous Cyanobacterium Arthrospira is commercially produced and is a functional, high-value, health food. We identified 5 low temperature and low light intensity tolerant strains of Arthrospira sp. (GMPA1, GMPA7, GMPB1, GMPC1, and GMPC3) using ethyl methanesulfonate mutagenesis and low temperature screening. The 5 Arthrospira strains grew rapidly below 14 °C, 43.75 μmol photons m(-2) s(-1) and performed breed conservation at 2.5 °C, 8.75 μmol photons m(-2) s(-1). We used morphological identification and molecular genetic analysis to identify GMPA1, GMPA7, GMPB1 and GMPC1 as Arthrospira platensis, while GMPC3 was identified as Arthrospira maxima...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28063102/quantitative-analyses-of-the-bacterial-microbiota-of-rearing-environment-tilapia-and-common-carp-cultured-in-earthen-ponds-and-inhibitory-activity-of-its-lactic-acid-bacteria-on-fish-spoilage-and-pathogenic-bacteria
#3
Pierre Marie Kaktcham, Jules-Bocamdé Temgoua, François Ngoufack Zambou, Gloria Diaz-Ruiz, Carmen Wacher, María de Lourdes Pérez-Chabela
The present study aimed to evaluate the bacterial load of water, Nile Tilapia and common Carp intestines from earthen ponds, isolate lactic acid bacteria (LAB) and assess their antimicrobial activity against fish spoilage and pathogenic bacteria. Following enumeration and isolation of microorganisms the antimicrobial activity of the LAB isolates was evaluated. Taxonomic identification of selected antagonistic LAB strains was assessed, followed by partial characterisation of their antimicrobial metabolites. Results showed that high counts (>4 log c...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28063101/elimination-and-molecular-identification-of-endophytic-bacterial-contaminants-during-in-vitro-propagation-of-bambusa-balcooa
#4
Syandan Sinha Ray, Md Nasim Ali, Shibasis Mukherjee, Gautam Chatterjee, Maitreyi Banerjee
Bambusa balcooa is an economically important, multipurpose bamboo species, decidedly used in construction industry. Availability of natural bamboo is depleting very rapidly due to accelerated deforestation and its unrestrained use. The large number and timely supply of saplings are the need of the hour for the restoration of bamboo stands. Micropropagation, being the potent alternative for season independent rapid regeneration, is restricted in bamboo because of endophytic contamination. An in vitro attempt has been taken to overcome the endophytic contamination by using broad spectrum antibiotics as surface sterilant as well as a media component...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28058639/selection-of-an-efficient-promoter-and-its-application-in-toyocamycin-production-improvement-in-streptomyces-diastatochromogenes-1628
#5
Xianhao Xu, Juan Wang, Andreas Bechthold, Zheng Ma, Xiaoping Yu
The selection of efficient promoter is usually very crucial for gene expression and metabolic engineering in Streptomycetes. In this study, the synthetic promoters SPL-57and SPL-21, and the engineered promoter kasOp(*)were selected and their activities were examined by using a reporter gene assay based on GUS. All selected promoters which have been reported to be stronger than promoter permE(*), which was used as control promoter. As host we were choosing S. diastatochromogenes 1628, the producer of toyocamycin (TM)...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28058638/functional-analysis-of-the-two-cyclophilin-isoforms-of-sinorhizobium-meliloti
#6
Eirini-Evangelia Thomloudi, Aggeliki Skagia, Anastasia Venieraki, Panagiotis Katinakis, Maria Dimou
The nitrogen fixing Sinorhizobium meliloti possesses two genes, ppiA and ppiB, encoding two cyclophilin isoforms which belong to the superfamily of peptidyl prolyl cis/trans isomerases (PPIase, EC: 5.2.1.8). Here, we functionally characterize the two proteins and we demonstrate that both recombinant cyclophilins are able to isomerise the Suc-AAPF-pNA synthetic peptide but neither of them displays chaperone function in the citrate synthase thermal aggregation assay. Furthermore, we observe that the expression of both enzymes increases the viability of E...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28058637/efficient-screening-of-potential-cellulases-and-hemicellulases-produced-by-bosea-sp-fbzp-16-using-the-combination-of-enzyme-assays-and-genome-analysis
#7
Aicha Asma Houfani, Tomáš Větrovský, Petr Baldrian, Said Benallaoua
Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044276/antimicrobial-potential-and-taxonomic-investigation-of-piezotolerant-streptomyces-sp-niot-ch-40-isolated-from-deep-sea-sediment
#8
Vishnu Priya Padmanaban, Pankaj Verma, Srividhyalakshmi Venkatabaskaran, Thirupathi Keppayan, Dharani Gopal, Ashok Kumar Sekar, Kirubagaran Ramalingam
Microbial-derived natural products from extreme niches such as deepsea are known to possess structural and functional novelty. With this background, the present study was designed to investigate the bioprospecting potential and systematics of a deep-sea derived piezotolerant bacterial strain NIOT-Ch-40, showing affiliation to the genus Streptomyces based on 16S RNA gene similarity. Preliminary screening for the presence of biosynthetic genes like polyketide synthase I, polyketide synthase II, non ribosomal peptide synthase, 3-amino-5-hydroxybenzoic acid synthase and spiroindimicin followed by antibacterial activity testing confirmed the presence of potent bioactivity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044275/improvement-of-shikimic-acid-production-in-escherichia-coli-with-growth-phase-dependent-regulation-in-the-biosynthetic-pathway-from-glycerol
#9
Ming-Yi Lee, Wen-Pin Hung, Shu-Hsien Tsai
Shikimic acid is an important metabolic intermediate with various applications. This paper presents a novel control strategy for the construction of shikimic acid producing strains, without completely blocking the aromatic amino acid biosynthesis pathways. Growth phase-dependent expression and gene deletion was performed to regulate the aroK gene expression in the shikimic acid producing Escherichia coli strain, SK4/rpsM. In this strain, the aroL and aroK genes were deleted, and the aroB, aroG*, ppsA, and tktA genes were overexpressed...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044274/bioremediation-of-copper-contaminated-soils-by-bacteria
#10
REVIEW
Jean-Yves Cornu, David Huguenot, Karine Jézéquel, Marc Lollier, Thierry Lebeau
Although copper (Cu) is an essential micronutrient for all living organisms, it can be toxic at low concentrations. Its beneficial effects are therefore only observed for a narrow range of concentrations. Anthropogenic activities such as fungicide spraying and mining have resulted in the Cu contamination of environmental compartments (soil, water and sediment) at levels sometimes exceeding the toxicity threshold. This review focuses on the bioremediation of copper-contaminated soils. The mechanisms by which microorganisms, and in particular bacteria, can mobilize or immobilize Cu in soils are described and the corresponding bioremediation strategies-of varying levels of maturity-are addressed: (i) bioleaching as a process for the ex situ recovery of Cu from Cu-bearing solids, (ii) bioimmobilization to limit the in situ leaching of Cu into groundwater and (iii) bioaugmentation-assisted phytoextraction as an innovative process for in situ enhancement of Cu removal from soil...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044273/rnd-efflux-pump-mediated-antibiotic-resistance-in-gram-negative-bacteria-escherichia-coli-and-pseudomonas-aeruginosa-a-major-issue-worldwide
#11
REVIEW
Minakshi Puzari, Pankaj Chetia
Therapeutic failures against diseases due to resistant Gram-negative bacteria have become a major threat nowadays as confirmed by surveillance reports across the world. One of the methods of development of multidrug resistance in Escherichia coli and Pseudomonas aeruginosa is by means of RND efflux pumps. Inhibition of these pumps might help to combat the antibiotic resistance problem, for which the structure and regulation of the pumps have to be known. Moreover, judicious antibiotic use is needed to control the situation...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044272/gene-cloning-and-expression-of-a-glucoside-3-dehydrogenase-from-sphingobacterium-faecium-zjf-d6-and-used-it-to-produce-n-p-nitrophenyl-3-ketovalidamine
#12
Jian-Fen Zhang, Wei-Qing Chen, Hong Chen
In this study, we report the cloning and expression of a functional glucoside 3-dehydrogenase (G3DH) gene from Sphingobacterium faecium ZJF-D6. This gene is 1686 bp in length and encodes a peptide of 562 amino acids. The G3DH gene was successfully expressed in E. coli, and the recombinant enzyme could oxidize glucosides, galactosides and analogues at C-3 position. The sequence and multiple alignment analysis showed that the enzyme has highest identity with G3DHs from Paraglaciecola polaris LMG 21857, Aliiglaciecola lipolytica E3 and Halomonas sp...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044271/the-application-of-powerful-promoters-to-enhance-gene-expression-in-industrial-microorganisms
#13
REVIEW
Shenghu Zhou, Guocheng Du, Zhen Kang, Jianghua Li, Jian Chen, Huazhong Li, Jingwen Zhou
Production of useful chemicals by industrial microorganisms has been attracting more and more attention. Microorganisms screened from their natural environment usually suffer from low productivity, low stress resistance, and accumulation of by-products. In order to overcome these disadvantages, rational engineering of microorganisms to achieve specific industrial goals has become routine. Rapid development of metabolic engineering and synthetic biology strategies provide novel methods to improve the performance of industrial microorganisms...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28044270/activation-of-a-calcium-dependent-protein-kinase-involved-in-the-azospirillum-growth-promotion-in-rice
#14
Claudia M Ribaudo, José A Curá, María L Cantore
Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca(2+)-dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western-blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27909993/impact-of-culture-conditions-on-the-chlorophyll-content-of-microalgae-for-biotechnological-applications
#15
REVIEW
Veronica da Silva Ferreira, Celso Sant'Anna
Chlorophyll is a commercially important natural green pigment responsible for the absorption of light energy and its conversion into chemical energy via photosynthesis in plants and algae. This bioactive compound is widely used in the food, cosmetic, and pharmaceutical industries. Chlorophyll has been consumed for health benefits as a nutraceutical agent with antioxidant, anti-inflammatory, antimutagenic, and antimicrobial properties. Microalgae are photosynthesizing microorganisms which can be extracted for several high-value bioproducts in the biotechnology industry...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27905092/broad-spectrum-of-antimicrobial-properties-of-commercial-wines-from-different-vitis-vinifera-l-varieties
#16
Aleksandra Radovanović, Biljana Arsić, Vladimir Radovanović, Branimir Jovančičević, Vesna Nikolić
Fifteen commercial wines produced from international and autochthonic varieties of Vitis vinifera L. cultivation of different Balkan winegrowing subregions were studied for their antimicrobial activity against six Gram-positive (Clostridium perfringens, Bacillus subtilis, Staphylococcus aureus, Listeria inocua, Sarcina lutea and Micrococcus flavus) and six Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella sonnei, Klebsiella pneumonia and Proteus vulgaris) bacteria. The concentrations and types of phenolic compounds responsible for antibacterial activity in wines were investigated by HPLC and spectroscopic methods...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27905091/recent-advances-of-molecular-toolbox-construction-expand-pichia-pastoris-in-synthetic-biology-applications
#17
REVIEW
Zhen Kang, Hao Huang, Yunfeng Zhang, Guocheng Du, Jian Chen
Pichia pastoris: (reclassified as Komagataella phaffii), a methylotrophic yeast strain has been widely used for heterologous protein production because of its unique advantages, such as readily achievable high-density fermentation, tractable genetic modifications and typical eukaryotic post-translational modifications. More recently, P. pastoris as a metabolic pathway engineering platform has also gained much attention. In this mini-review, we addressed recent advances of molecular toolboxes, including synthetic promoters, signal peptides, and genome engineering tools that established for P...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27900629/dracorhodin-perochlorate-attenuates-staphylococcus-aureus-usa300-virulence-by-decreasing-%C3%AE-toxin-expression
#18
Yumin Liu, Dongxue Shi, Yan Guo, Meng Li, Yonghong Zha, Quankai Wang, Jianfeng Wang
α-Toxin, a pore-forming toxin secreted by most Staphylococcus aureus, plays critical role in the pathogenesis associated with various infectious diseases. The USA300 which is a major international epidemic methicilin-resisrant S. aureus has spread rapidly to multiple countries and become an emerging public health concern. In this study, the in vitro efficacy of Dracorhodin Perochlorate (DP) against USA300 virulence was evaluated. Using susceptibility testing, immunoblots, rabbit blood haemolytic assay and real-time RT-PCR, we observed that the α-toxin production was decreased when USA300 was co-cultured with different sub-inhibitory concentration of DP...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27896581/secondary-metabolites-of-endophytic-xylaria-species-with-potential-applications-in-medicine-and-agriculture
#19
REVIEW
Martha Lydia Macías-Rubalcava, Rosa Elvira Sánchez-Fernández
Fungal endophytes are important sources of bioactive secondary metabolites. The genus Xylaria Hill (ex Schrank, 1789, Xylariaceae) comprises various endophytic species associated to both vascular and non vascular plants. The secondary metabolites produced by Xylaria species include a variety of volatile and non-volatile compounds. Examples of the former are sesquiterpenoids, esters, and alcohols, among others; and of the latter we find terpenoids, cytochalasins, mellein, alkaloids, polyketides, and aromatic compounds...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27896580/highly-improved-acarbose-production-of-actinomyces-through-the-combination-of-artp-and-penicillin-susceptible-mutant-screening
#20
Fei Ren, Long Chen, Qunyi Tong
Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis...
January 2017: World Journal of Microbiology & Biotechnology
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