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World Journal of Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/28197940/the-change-of-the-state-of-cell-membrane-can-enhance-the-synthesis-of-menaquinone-in-escherichia-coli
#1
Yan Liu, Xiu-Min Ding, Zheng-Lian Xue, Liu-Xiu Hu, Ning-Juan Zhang, Zhou Wang, Jian-Wei Yang, Qian Cheng, Ming-Hong Chen, Zhuang-Zhuang Zhang, Zhi-Ming Zheng
Menaquinone (MK) was an attractive membrane-bound intracellular chemical. To enhance its production, we tried to find the relationship between its synthesis and the state of cell membrane in producing strain. Due to non-ionic surfactant-polyoxyethylene oleyl ether (POE) and plant oil-cedar wood oil (CWO) can typically increase extracellular secretion and intracellular synthesis of MK respectively, the effect of these two substances on cell morphology, physical properties of cell membrane was investigated. Finally, two engineering strains were constructed to verify whether the state of cell membrane can enhance MK synthesis...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28190182/progress-and-perspectives-on-improving-butanol-tolerance
#2
REVIEW
Siqing Liu, Nasib Qureshi, Stephen R Hughes
Fermentative production of butanol for use as a biofuel or chemical feedstock is regarded as a promising renewable technology that reduces greenhouse gas emissions and has the potential to become a substitute for non-sustainable chemical production route. However, butanol toxicity to the producing microbes remains a barrier to achieving sufficiently high titers for cost-effective butanol fermentation and recovery. Investigations of the external stress of high butanol concentration on butanol-producing microbial strains will aid in developing improved microbes with increased tolerance to butanol...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28188589/synergistic-activity-of-sub-inhibitory-concentrations-of-curcumin-with-ceftazidime-and-ciprofloxacin-against-pseudomonas-aeruginosa-quorum-sensing-related-genes-and-virulence-traits
#3
Shekoufeh Roudashti, Habib Zeighami, Hesam Mirshahabi, Shahin Bahari, Ali Soltani, Fakhri Haghi
Quorum sensing (QS) system in Pseudomonas aeruginosa may be an important target for pharmacological intervention. The present study aimed to investigate the synergetic activity of sub-MIC concentrations of curcumin (C) with ceftazidime (CAZ) and ciprofloxacin (CIP) against P. aeroginusa QS system. We determined the MIC and synergistic activity of C, CAZ and CIP against P. aeroginusa PAO1 using broth microdilution and checkerboard titration methods. The activity of sub-MIC (1/4 and 1/16 MIC) concentrations of C on the QS signal molecules was assessed using a reporter strain assay...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28181116/diverse-microbial-communities-in-non-aerated-compost-teas-suppress-bacterial-wilt
#4
W K Mengesha, S M Powell, K J Evans, K M Barry
Non-aerated compost teas (NCTs) are water extracts of composted organic materials and are used to suppress soil borne and foliar disease in many pathosystems. Greenhouse trials were used to test the effectiveness of NCTs to suppress potato bacterial wilt caused by Ralstonia solanacearum on plants grown in soils inoculated with a virulent isolate of the pathogen (biovar II). NCTs prepared from matured compost sources: agricultural waste (AWCT), vermicompost (VCT) and solid municipal waste (SMWCT) were evaluated at three initial application times (7 days before inoculation, at time of inoculation and 7 days after inoculation) prior to weekly applications, in a randomized complete-block design...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28176202/bioethanol-production-from-steam-exploded-rice-husk-by-recombinant-escherichia-coli-ko11
#5
Takamitsu Tabata, Yusuke Yoshiba, Tomonori Takashina, Kazuo Hieda, Norio Shimizu
Rice husk is one of the most abundant types of lignocellulosic biomass. Because of its significant amount of sugars, such as cellulose and hemicellulose, it can be used for the production of biofuels such as bioethanol. However, the complex structure of lignocellulosic biomass, consisting of cellulose, hemicellulose and lignin, is resistant to degradation, which limits biomass utilization for ethanol production. The protection of cellulose by lignin contributes to the recalcitrance of lignocelluloses to hydrolysis...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28176201/lactobacillus-delbrueckii-subsp-lactis-strain-cidca-133-stimulates-murine-macrophages-infected-with-citrobacter-rodentium
#6
Ayelén A Hugo, Ivanna S Rolny, David Romanin, Pablo F Pérez
Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phagocytic cells could contribute to improve host´s defences against C. rodentium, we investigated the effect of Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) on the interaction between murine macrophages (RAW 264...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28168624/antimicrobial-activity-of-endophytic-fungi-from-olive-tree-leaves
#7
Cynthia Malhadas, Ricardo Malheiro, José Alberto Pereira, Paula Guedes de Pinho, Paula Baptista
In this study, the antimicrobial potential of three fungal endophytes from leaves of Olea europaea L. was evaluated and the host plant extract effect in the antimicrobial activity was examined. The volatile compounds produced by endophytes were identified by GC/MS and further correlated with the antimicrobial activity. In potato dextrose agar, both Penicillium commune and Penicillium canescens were the most effective inhibiting Gram-positive and -negative bacteria (up to 2.7-fold compared to 30 µg/mL chloramphenicol), whereas Alternaria alternata was most effective inhibiting yeasts (up to 8...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28161849/survival-strategies-of-escherichia-coli-and-vibrio-spp-contribution-of-the-viable-but-nonculturable-phenotype-to-their-stress-resistance-and-persistence-in-adverse-environments
#8
REVIEW
M Orruño, V R Kaberdin, I Arana
In their natural ecosystems, bacteria are continuously exposed to changing environmental factors including physicochemical parameters (e.g. temperature, pH, etc.), availability of nutrients as well as interaction(s) with other organisms. To increase their tolerance and survival under adverse conditions, bacteria trigger a number of adaptation mechanisms. One of the well-known adaptation responses of the non-spore-forming bacteria is the acquisition of the viable but non-culturable (VBNC) state. This phenotype is induced by different stress factors (e...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28161848/eukaryotic-molecular-diversity-at-different-steps-of-the-wastewater-treatment-plant-process-reveals-more-phylogenetic-novel-lineages
#9
Rakia Chouari, Marie Leonard, Moez Bouali, Sonda Guermazi, Naima Rahli, Ines Zrafi, Loïc Morin, Abdelghani Sghir
Wastewater microbiota represents important actors of organic depollution. Nowadays, some species used as bioindicators of the effluent quality are still identified by microscopy. In the present study, we investigated eukaryotic diversity at the different steps of the treatment process of a wastewater treatment plant (aerobic, anaerobic, clarifier basins and anaerobic digester) using the 18S rRNA gene sequencing approach. Of the 1519 analysed sequences, we identified 160 operational taxonomic units. Interestingly, 56...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28160118/production-of-chemicals-from-c1-gases-co-co2-by-clostridium-carboxidivorans
#10
REVIEW
Ánxela Fernández-Naveira, Haris Nalakath Abubackar, María C Veiga, Christian Kennes
Bioprocesses in conventional second generation biorefineries are mainly based on the fermentation of sugars obtained from lignocellulosic biomass or agro-industrial wastes. An alternative to this process consists in gasifying those same feedstocks or even other carbon-containing materials to obtain syngas which can also be fermented by some anaerobic bacteria to produce chemicals or fuels. Carbon monoxide, carbon dioxide and hydrogen, which are the main components of syngas, are also found in some industrial waste gases, among others in steel industries...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28160117/characterization-of-salmonella-spp-from-wastewater-used-for-food-production-in-morogoro-tanzania
#11
Ofred J Mhongole, Robinson H Mdegela, Lughano J M Kusiluka, Anita Forslund, Anders Dalsgaard
Wastewater use for crop irrigation and aquaculture is commonly practiced by communities situated close to wastewater treatment ponds. The objective of this study was to characterize Salmonella spp. and their antimicrobial susceptibility patterns among isolates from wastewater and Tilapia fish. A total of 123 Salmonella spp. isolates were isolated from 52 water and 21 fish intestinal samples. Genotyping of Salmonella spp. isolates was done by Pulsed-field Gel Electrophoresis (PFGE). Antimicrobial susceptibility testing was done by the minimal inhibitory concentration (MIC) technique...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120312/nitrogen-fixing-bacterial-diversity-in-a-tropical-estuarine-sediments
#12
Jabir Thajudeen, Jesmi Yousuf, Vipindas Puthiya Veetil, Sherin Varghese, Arvind Singh, Mohamed Hatha Abdulla
Microorganisms play a significant role in biogeochemical cycles, especially in the benthic and pelagic ecosystems. Role of environmental parameters in regulating the diversity, distribution and physiology of these microorganisms in tropical marine environment is not well understood. In this study, we have identified dinitrogen (N2) fixing bacterial communities in the sediments by constructing clone libraries of nitrogenase (nifH) gene from four different stations in the Cochin estuary, along the southeastern Arabian Sea...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120311/a-new-recombinant-endo-1-3-%C3%AE-d-glucanase-from-the-marine-bacterium-formosa-algae-kmm-3553-enzyme-characteristics-and-transglycosylation-products-analysis
#13
Mikhail I Kusaykin, Alexey A Belik, Svetlana N Kovalchuk, Pavel S Dmitrenok, Valerii A Rasskazov, Vladimir V Isakov, Tatyana N Zvyagintseva
A specific endo-1,3-β-D-glucanase (GFA) gene was found in genome of marine bacterium Formosa algae KMM 3553. For today this is the only characterized endo-1,3-β-D-glucanase (EC 3.2.1.39) in Formosa genus and the only bacterial EC 3.2.1.39 GH16 endo-1,3-β-D-glucanase with described transglycosylation activity. It was expressed in E. coli and isolated in homogeneous state. Investigating the products of polysaccharides digestion with GFA allowed to establish it's substrate specificity and classify this enzyme as glucan endo-1,3-β-D-glucosidase (EC 3...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120310/microbial-strategy-for-potential-lead-remediation-a-review-study
#14
REVIEW
Xiaohong Pan, Zhi Chen, Lan Li, Wenhua Rao, Zhangyan Xu, Xiong Guan
The extensive exploitation and usage of lead compounds result in severe lead(II) pollution in water and soil environments, even in agricultural land, threatening the health of animals and humans via food chains. The recovery and remediation of lead(II) from water and soil environments have been intensively concerned in recent years. Compared with the traditional physic-chemistry treatment, microbial remediation strategy is a promising alternative to remediate lead(II)-contaminated environments due to its cost-effective and environmentally-friendly properties...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120309/microbial-production-of-astilbin-a-bioactive-rhamnosylated-flavanonol-from-taxifolin
#15
Nguyen Huy Thuan, Sailesh Malla, Nguyen Thanh Trung, Dipesh Dhakal, Anaya Raj Pokhrel, Luan Luong Chu, Jae Kyung Sohng
Flavonoids are plant-based polyphenolic biomolecules with a wide range of biological activities. Glycosylated flavonoids have drawn special attention in the industries as it improves solubility, stability, and bioactivity. Herein, we report the production of astilbin (ATN) from taxifolin (TFN) in genetically-engineered Escherichia coli BL21(DE3). The exogenously supplied TFN was converted to ATN by 3-O-rhamnosylation utilizing the endogeneous TDP-L-rhamnose in presence of UDP-glycosyltransferase (ArGT3, Gene Bank accession number: At1g30530) from Arabidopsis thaliana...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120308/seasonal-detection-of-atrazine-and-atza-in-man-made-waterways-receiving-agricultural-runoff-in-a-subtropical-semi-arid-environment-hidalgo-county-texas-usa
#16
Ibdanelo Cortez, Christopher J Vitek, Michael W Persans, Kristine L Lowe
Atrazine is a widely-used herbicide that can impact non-target organisms in the environment but can be biologically degraded by several types of microorganisms. In this study, the gene atzA, which encodes for the initial step in bacterially-mediated atrazine degradation, was used as an indicator of atrazine pollution in agricultural canals located in Hidalgo County, Texas, USA. The concentration of atrazine and atzA were monitored once per month for 12 months during 2010-2011. Atrazine was measured using an enzyme-linked immunosorbent assay; atzA abundance was monitored using Quantitative Polymerase Chain Reaction (Q-PCR) analyses...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120307/performance-of-the-biological-aerated-filter-bioaugmented-by-a-yeast-magnusiomyces-ingens-lh-f1-for-treatment-of-acid-red-b-and-microbial-community-dynamics
#17
Muyang He, Liang Tan, Shuxiang Ning, Li Song, Shengnan Shi
Biological aerated filters (BAFs) were constructed and operated for assessing the effectiveness of bacterial community bioaugmented by a yeast Magnusiomyces ingens LH-F1 for treatment of azo dye Acid Red B (ARB). Dynamics of both bacterial and fungal communities were analyzed through MiSeq sequencing method. The results showed that the bioaugmented BAF displayed obviously better performance for decolorization, COD removal and detoxification of ARB wastewater than the other two which were inoculated with activated sludge (AS) and single M...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28120306/cellulase-with-high-%C3%AE-glucosidase-activity-by-penicillium-oxalicum-under-solid-state-fermentation-and-its-use-in-hydrolysis-of-cassava-residue
#18
Lin-Hui Su, Shuai Zhao, Sui-Xin Jiang, Xu-Zhong Liao, Cheng-Jie Duan, Jia-Xun Feng
In this study, we investigated cellulase production by Penicillium oxalicum EU2106 under solid-state fermentation (SSF) and its hydrolysis efficiency toward NaOH-H2O2-pretreated cassava residue (NHCR) produced after bioethanol fermentation. Optimization of SSF cultivation conditions for P. oxalicum EU2106 using a Box-behnken design-based response-surface methodology resulted in maximal cellulase activity of 34.0 ± 2.8 filter-paper units/g dry substrate, exhibiting a ~ twofold increase relative to activities obtained under non-optimized conditions...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28102516/production-of-lovastatin-and-itaconic-acid-by-aspergillus-terreus-a-comparative-perspective
#19
REVIEW
Tomasz Boruta, Marcin Bizukojc
Aspergillus terreus is a textbook example of an industrially relevant filamentous fungus. It is used for the biotechnological production of two valuable metabolites, namely itaconic acid and lovastatin. Itaconic acid serves as a precursor in polymer industry, whereas lovastatin found its place in the pharmaceutical market as a cholesterol-lowering statin drug and a precursor for semisynthetic statins. Interestingly, their biosynthetic gene clusters were shown to reside in the common genetic neighborhood. Despite the genomic proximity of the underlying biosynthetic genes, the production of lovastatin and itaconic acid was shown to be favored by different factors, especially with respect to pH values of the broth...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28074411/biomass-and-terpenoids-produced-by-mutant-strains-of-arthrospira-under-low-temperature-and-light-conditions
#20
Jian Guan, Songdong Shen, Hao Wu, Xin Liu, Weijie Shen, Yuan He, R Duan
The filamentous Cyanobacterium Arthrospira is commercially produced and is a functional, high-value, health food. We identified 5 low temperature and low light intensity tolerant strains of Arthrospira sp. (GMPA1, GMPA7, GMPB1, GMPC1, and GMPC3) using ethyl methanesulfonate mutagenesis and low temperature screening. The 5 Arthrospira strains grew rapidly below 14 °C, 43.75 μmol photons m(-2) s(-1) and performed breed conservation at 2.5 °C, 8.75 μmol photons m(-2) s(-1). We used morphological identification and molecular genetic analysis to identify GMPA1, GMPA7, GMPB1 and GMPC1 as Arthrospira platensis, while GMPC3 was identified as Arthrospira maxima...
February 2017: World Journal of Microbiology & Biotechnology
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