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World Journal of Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/30547239/cyanase-independent-utilization-of-cyanate-as-a-nitrogen-source-in-ascomycete-yeasts
#1
Tomas Linder
The occurrence of putative cyanases (EC 4.2.1.104) in the genomes of yeasts belonging to the ascomycete sub-phyla Saccharomycotina (budding yeasts) and Taphrinomycotina (fission yeasts) was investigated. Predicted gene products displaying significant sequence similarity to previously characterized cyanases were identified in the genomes of the budding yeast Lipomyces starkeyi and the fission yeasts Protomyces lactucaedebilis, Saitoella complicata and Taphrina deformans. Li. starkeyi and Sai. complicata were further tested for their ability to utilize cyanate as a nitrogen source...
December 13, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30536084/isolation-and-characterization-of-mineral-dissolving-bacteria-from-different-levels-of-altered-mica-schist-surfaces-and-the-adjacent-soil
#2
Yuan Li Wang, Qi Wang, Rui Yuan, Xia Fang Sheng, Lin Yan He
Microorganisms play important role in mineral weathering. However, little is known about rock-associated mineral-dissolving bacteria. In this study, 129 bacterial isolates were obtained from the less and more weathered mica schist surfaces and the adjacent soil and characterized for mineral dissolving activity, population, and the linkage of rock weathering level and distribution of the bacteria. Among the 129 isolates, 112 isolates could dissolve biotite. The relative abundance of the highly effective Fe solubilizers was significantly higher on the more altered rock surface (89...
December 10, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30535777/repair-characteristics-and-time-dependent-effects-in-saccharomyces-cerevisiae-cells-after-x-ray-irradiation
#3
Xiaopeng Guo, Miaomiao Zhang, Ruiyuan Liu, Yue Gao, Yang Yang, Wenjian Li, Dong Lu
In this study, we examined the dynamics of phenotypic and transcriptional profiles in Saccharomyces cerevisiae following semi-lethal X-ray irradiation. Post-irradiation, reproductive death was revealed as the predominant form of death in S. cerevisiae and almost all the irradiated cells were physically present and intact. In addition, cell cycle arrest reached its peak and cell division was at its valley at 2 h. Cell cycle arrest, cell division potential, DNA damage, and mitochondrial transmembrane potential (MTP) showed significant recovery at 4 h (P > 0...
December 10, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30511216/co-production-of-ampc-and-extended-spectrum-beta-lactamases-in-cephalosporin-resistant-acinetobacter-baumannii-in-egypt
#4
Heba Shehta Said, Abdalbagi Basheer Benmahmod, Ramadan Hassan Ibrahim
Acinetobacter baumannii is an opportunistic pathogen that has been held responsible for a lot of infections worldwide. Infections caused by this pathogen are difficult to control because of the widespread of antimicrobial resistance mechanisms. The aim of the present study is to assess the prevalence of extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases among isolates of A. baumannii collected from different clinical sources in Mansoura University Hospitals, Egypt. Antimicrobial susceptibility testing has demonstrated elevated resistance level to β-lactams, quinolones and aminoglycosides...
December 3, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30511184/diversity-and-seasonal-distribution-of-ammonia-oxidizing-archaea-in-the-water-column-of-a-tropical-estuary-along-the-southeast-arabian-sea
#5
Puthiya Veettil Vipindas, Thajudeen Jabir, Chekidhenkuzhiyil Jasmin, Tharakan Balu, Thekkendavida Velloth Rehitha, Balakrishnan Meenakshikutty Adarsh, Shanta Nair, Mohamed Hatha Abdulla, Anas Abdulaziz
Diversity and distribution pattern of ammonia-oxidizing archaea (AOA) were studied across a salinity gradient in the water column of Cochin Estuary (CE), a tropical monsoonal estuary along the southeast Arabian Sea. The water column of CE was found to be nutrient rich with high bacterial (3.7-6.7 × 108  cells L-1 ) and archaeal abundance (1.9-4.5 × 108  cells L-1 ). Diversity and seasonal variation in the distribution pattern of AOA were studied using clone library analysis and Denaturing gradient gel electrophoresis (DGGE)...
December 3, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30506400/global-insight-into-the-distribution-of-velvet-like-b-protein-in-cochliobolus-species-and-implication-in-pathogenicity-and-fungicide-resistance
#6
REVIEW
Louis Bengyella
The Cochliobolus genus consist of over 55 species among which the 5 most devastating are Cochliobolus carbonum, Cochliobolus heterostrophus, Cochliobolus miyabeanus, Crocus sativus and Cochliobolus lunatus causing damages in sorghum, wheat, rice, maize, cassava and soybean estimated at over 10 billion USD per annum worldwide. The dynamic pathogenicity of Cochliobolus species and the plethora of infected hosts is determined by the evolution of virulence determinants such as the velvet-like B protein (VelB)...
December 1, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30506306/screening-of-plant-growth-promotion-ability-among-bacteria-isolated-from-field-grown-sorghum-under-different-managements-in-brazilian-drylands
#7
Jéssica Fernanda da Silva, Thaise Rosa da Silva, Indra Elena Costa Escobar, Ana Carla Resende Fraiz, Jonnathan Whiny Moraes Dos Santos, Tailane Ribeiro do Nascimento, João Marcos Rodrigues Dos Santos, Samuel James Windsor Peters, Roseli Freire de Melo, Diana Signor, Paulo Ivan Fernandes-Júnior
Sorghum [Sorghum bicolor (L.) Moench] is a multipurpose grass cultivated in drylands due to its adaptation to drought. However the characteristics of sorghum-associated bacteria are not known in the Brazilian drylands. The aim of this study was to isolate and evaluate the plant growth promotion potential bacteria from field-grown sorghum under two irrigation and manure application levels in a Brazilian semi-arid reagion. Sorghum was irrigated with 3 or 1 mm day-1 and fertilized or not with liquid goat manure...
November 30, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30506280/inducing-flocculation-of-non-floc-forming-escherichia-coli-cells
#8
REVIEW
Yoshihiro Ojima, Masayuki Azuma, Masahito Taya
The present article reviews several approaches for inducing flocculation of Escherichia coli cells. The common industrially used bacterium E. coli does not naturally have floc-forming ability. However, there are several approaches to induce flocculation of E. coli cells. One is induction by flocculants-polyvalent inorganic salts, synthetic polymeric flocculants, or bio-based polymeric materials, including polysaccharide derivatives. Another method is the induction of spontaneous flocculation by changing the phenotypes of E...
November 30, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30488133/posttranslational-modification-of-dinitrogenase-reductase-in-rhodospirillum-rubrum-treated-with-fluoroacetate
#9
Natalia Akentieva
Nitrogen fixation is one of the major biogeochemical contributions carried out by diazotrophic microorganisms. The goal of this research is study of posttranslational modification of dinitrogenase reductase (Fe protein), the involvement of malate and pyruvate in generation of reductant in Rhodospirillum rubrum. A procedure for the isolation of the Fe protein from cell extracts was developed and used to monitor the modification of the Fe protein in vivo. The subunit pattern of the isolated the Fe protein after sodium dodecyl sulfate-polyacrylamide gel electrophoresis was assayed by Western blot analysis...
November 28, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30478689/a-transcriptome-analysis-of-the-ameliorate-effect-of-cyclocarya-paliurus-triterpenoids-on-ethanol-stress-in-saccharomyces-cerevisiae
#10
Yuhui Chen, Xin Zhang, Man Zhang, Jieyu Zhu, Zufang Wu, Xiaojie Zheng
Saccharomyces cerevisiae (S. cerevisiae) plays a critical role in ethanol fermentation. However, during the fermentation, yeast cells are exposed to the accumulation of ethanol, which significantly affect the cell growth and the target product yield. In the present work, we employed RNA-sequence (RNA-seq) to investigate the ameliorate effect of Cyclocarya paliurus (C. paliurus) triterpenoids on S. cerevisiae under the ethanol stress. After C. paliurus triterpenoids intervention (0.3% v/v), 84 differentially expressed genes (DEGs) were identified, including 39 up-regulated and 45 down-regulated genes...
November 26, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30478596/manipulation-of-the-microalgal-chloroplast-by-genetic-engineering-for-biotechnological-utilization-as-a-green-biofactory
#11
REVIEW
Yong Min Kwon, Kyung Woo Kim, Tae-Young Choi, Sun Young Kim, Jaoon Young Hwan Kim
The chloroplast is an essential organelle in microalgae for conducting photosynthesis, thus enabling the photoautotrophic growth of microalgae. In addition to photosynthesis, the chloroplast is capable of various biochemical processes for the synthesis of proteins, lipids, carbohydrates, and terpenoids. Due to these attractive characteristics, there has been increasing interest in the biotechnological utilization of microalgal chloroplast as a sustainable alternative to the conventional production platforms used in industrial biotechnology...
November 26, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30474742/expression-purification-and-evaluation-of-in-vivo-anti-fibrotic-activity-for-soluble-truncated-tgf-%C3%AE-receptor-ii-as-a-cleavable-his-sumo-fusion-protein
#12
Xiaohua Wang, Yuting Li, Xin Li, Lei Yan, Huilin Guan, Ruijie Han, Yang Han, Jinqiu Gui, Xiaoyan Xu, Yan Dong, Haifeng Liu
Excessive production of transforming growth factor-β1 (TGF-β1) and its binding to transforming growth factor-β receptor type II (TGF-βRII) promotes fibrosis by activation of the TGF-β1-mediated signaling pathway. Thus, the truncated extracellular domain of TGF-βRII (tTβRII) is a promising anti-fibrotic candidate, as it lacks the signal transduction domain. In this work, the native N-terminal tTβRII was prepared as a His-SUMO fusion protein (termed His-SUMO-tTβRII) in Escherichia coli strain BL21 (DE3)...
November 24, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30465084/evaluation-of-the-sub-optimal-induction-strategies-for-heterologous-proteins-production-by-pichia-pastoris-mut-mut-s-strains-and-related-transcriptional-and-metabolic-analysis
#13
Luqiang Jia, Minjie Gao, Jian Yan, Shanshan Chen, Jiaowen Sun, Qiang Hua, Jian Ding, Zhongping Shi
Heterologous proteins induction by methylotrophic recombinant Pichia pastoris is generally implemented at high cells density condition. Methanol concentration (MeOH) and dissolved oxygen concentration (DO) are two crucial operating parameters controlling proteins production. It is difficult to control MeOH/DO at their desired levels simultaneously due to the extremely high oxygen consumption features. Methanol utilization plus (Mut+ ) and slow (MutS ) strains are the two typical phenotypes of recombinant P...
November 21, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30456633/atmt-transformation-efficiencies-with-native-promoters-in-botryosphaeria-kuwatsukai-causing-ring-rot-disease-in-pear
#14
Xueying Gu, Jiamin Zhao, Hongkai Wang, Fu-Cheng Lin, Qingyuan Guo, Neeraj Shrivastava, Rajesh Jeewon
Botryosphaeria kuwatsukai is an important fungal pathogen affecting pear fruits. However, infection processes of this fungus are still unclear. This study seeks to develop the fungal transformation of B. kuwatsukai by Agrobacterium tumefaciens-mediated transformation (ATMT), assess the reliability of appropriate vectors and examine the infection processes in vitro using a GFP labeled strain of B. kuwatsukai. To establish a highly effective transformation system in B. kuwatsukai, binary vectors containing various lengths of H3 promoters and TEF promoters fused with GFP and hygromycin B resistance gene cassettes were constructed...
November 19, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30456518/medically-important-biofilms-and-non-thermal-plasma
#15
REVIEW
Jaroslav Julák, Vladimír Scholtz, Eva Vaňková
In recent decades, the non-thermal plasma, i.e. partially or completely ionized gas produced by electric discharges at ambient temperature, has become of interest for its microbiocidal properties with potential of use in the food industry or medicine. Recently, this interest focuses not only on the planktonic forms of microorganisms but also on their biofilms. The works in this interdisciplinary field are summarized in this review. The wide range of biofilm-plasma interactions is divided into studies of general plasma action on bacteria, on biofilm and on its oral and dental application; a short overview of plasma instrumentation is also included...
November 19, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30446973/isolation-and-characterization-of-aerobic-culturable-arsenic-tolerant-bacteria-from-lead-zinc-mine-tailing-in-southern-china
#16
Dan Wu, Zhipeng Zhang, Qinglong Gao, Yuchao Ma
Bioremediation of arsenic (As) pollution is an important environmental issue. The present investigation was carried out to isolate As-resistant novel bacteria and characterize their As transformation and tolerance ability. A total of 170 As-resistant bacteria were isolated from As-contaminated soils at the Kangjiawan lead-zinc tailing mine, located in Hunan Province, southern China. Thirteen As-resistant isolates were screened by exposure to 260 mM Na2 HAsO4 ·7H2 O, most of which showed a very high level of resistance to As5+ (MIC ≥ 600 mM) and As3+ (MIC ≥ 10 mM)...
November 16, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30446834/quick-and-accurate-detection-of-fusarium-oxysporum-f-sp-carthami-in-host-tissue-and-soil-using-conventional-and-real-time-pcr-assay
#17
Neeraja Singh, Rupam Kapoor
Safflower wilt, caused by Fusarium oxysporum f. sp. carthami (Foc) is a major limiting factor for safflower (Carthamus tinctorius) production worldwide. In India alone, about 40-80% disease incidence has been reported. A rapid, efficient, specific, and sensitive diagnostic technique for Foc is therefore crucial to manage Fusarium wilt of safflower. Twenty-five isolates of F. oxysporum formae speciales infecting other crops, 17 isolates of Fusarium spp. and seven isolates of other fungal pathogens of safflower along with 75 Foc isolates were used for identification of band specific to Foc using inter-simple sequence repeat (ISSR) analysis...
November 16, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30446833/fermentative-hydrogen-production-from-low-value-substrates
#18
REVIEW
Ahmed H S Hassan, Thorsten Mietzel, Ruth Brunstermann, Sebastian Schmuck, Jens Schoth, Marco Küppers, Renatus Widmann
Hydrogen is a promising energy source that is believed to replace the conventional energy sources e.g. fossil fuels over years. Hydrogen production methods can be divided into conventional production methods which depend mainly on fossil fuels and alternative production methods including electrolysis of water, biophotolysis and fermentation hydrogen production from organic waste materials. Compared to the conventional methods, the alternative hydrogen production methods are less energy intensive and negative-value substrates i...
November 16, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30446832/production-of-nanocalcite-crystal-by-a-urease-producing-halophilic-strain-of-staphylococcus-saprophyticus-and-analysis-of-its-properties-by-xrd-and-sem
#19
Gholam Reza Ghezelbash, Maryam Haddadi
Cementation of salt-containing soils can be achieved by salt-tolerant or halophilic calcite precipitation bacteria. Therefore, the isolation of calcite-producing bacteria in the presence of salt is the first step in the microbial cementation of saline soils. Urease producing bacteria can cause calcite nano-crystals to precipitate by producing urease in the presence of urea and calcium. The purpose of this study was to isolate urease producing halophilic bacteria in order to make calcite precipitate in saline soil...
November 16, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/30413892/molecular-identification-of-yeast-lactic-and-acetic-acid-bacteria-species-during-spoilage-of-tchapalo-a-traditional-sorghum-beer-from-c%C3%A3-te-d-ivoire
#20
Constant K Attchelouwa, Florent K N'guessan, Francine M D Aké, Marcellin K Djè
Yeasts, lactic and acetic acid bacteria are responsible of microbial spoilage of alcoholic beverages. However species involved in deterioration of sorghum beer produced in Côte d'Ivoire has not been investigated. This study was carried out to identify species of yeast, LAB and AAB during spoilage of tchapalo in order to define the best strategy for beer preservative. Thus, a total of 210 yeasts, LAB and AAB were isolated from samples of tchapalo stored at ambient temperature and at 4 °C for 3 days. Based on PCR-RFLP of the ITS region and the sequencing of D1/D2 domain, yeast isolates were assigned to seven species (Saccharomyces cerevisiae, Candida tropicalis, Rhodotorula mucilaginosa, Trichosporon asahii, Kluyveromyces marxianus, Meyerozyma guilliermondii and Trichosporon coremiiforme)...
November 9, 2018: World Journal of Microbiology & Biotechnology
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