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World Journal of Microbiology & Biotechnology

Fei-Hong Zhai, Hong-Yan Liu, Jian-Rong Han
Solid-state fermentation (SSF) with Agaricus brasiliensis and Agaricus bisporus on corn was carried out. The results showed that SSF with the two fungi made up the deficiency of tryptophan in corn and improved the protein nutritional value of corn. The conjugated polyphenols contents in fermented corn decreased and free polyphenols (FPP) contents increased. FPP contents in corn fermented with the two fungi reached respectively 25 and 88 times of control, total polyohenols contents reached respectively 1.4 and 3...
February 15, 2018: World Journal of Microbiology & Biotechnology
Issa Diédhiou, Diaga Diouf
Root endosymbioses are mutualistic interactions between plants and the soil microorganisms (Fungus, Frankia or Rhizobium) that lead to the formation of nitrogen-fixing root nodules and/or arbuscular mycorrhiza. These interactions enable many species to survive in different marginal lands to overcome the nitrogen-and/or phosphorus deficient environment and can potentially reduce the chemical fertilizers used in agriculture which gives them an economic, social and environmental importance. The formation and the development of these structures require the mediation of specific gene products among which the transcription factors play a key role...
February 15, 2018: World Journal of Microbiology & Biotechnology
Bin He, Yayi Tu, Zhihong Hu, Long Ma, Jing Dai, Xiaojie Cheng, Haoran Li, Lanlan Liu, Bin Zeng
The High osmolarity glycerol (HOG) gene family plays crucial roles in various developmental and physiological processes in fungi, such as the permeability of cell membrane, chlamydospore formation and stress signaling. Although the function of HOG genes has been investigated in Saccharomyces cerevisiae and some filamentous fungi, a comprehensive analysis of HOG gene family has not been performed in Aspergillus oryzae, a fungi mainly used for the production of soy sauce. In this study, we identified and corrected a total of 90 HOG genes from the A...
February 9, 2018: World Journal of Microbiology & Biotechnology
Jun Zheng, Jun-Qiao Feng, Lei Zhou, Serge Maurice Mbadinga, Ji-Dong Gu, Bo-Zhong Mu
Bacterial community and diversity in a long-term petroleum-contaminated soil of an oilfield were characterized using 16S rRNA gene-based Illumina MiSeq high-throughput sequencing. Results indicated that Proteobacteria (49.11%) and Actinobacteria (24.24%) were the most dominant phyla, and the most abundant genera were Pseudoxanthomonas (8.47%), Luteimonas (3.64%), Alkanindiges (9.76%), Acinetobacter (5.26%) and Agromyces (8.56%) in the soil. Meanwhile a series of cultivations were carried out for isolation of alkane degraders from petroleum-contaminated soil with gellan gum and agar as gelling agents...
February 9, 2018: World Journal of Microbiology & Biotechnology
Van Hong Thi Pham, Dhiraj Kumar Chaudhary, Seung-Woo Jeong, Jaisoo Kim
The aim of this study was to investigate oil-degrading ability of newly isolated strain Rhodococcus Y2-2 at low temperature. Rhodococcus sp. Y2-2 was isolated from oil-contaminated soil sampled at the end of winter using a newly developed transwell plate method. In the liquid phase, the oil-degradation efficiency of strain Rhodococcus sp. Y2-2 was about 84% with an initial concentration of 1500 ppm TPH (500 ppm each of kerosene, gasoline, and diesel) when incubated for 2 weeks under optimal conditions: 10 °C, pH 7, and 0...
February 6, 2018: World Journal of Microbiology & Biotechnology
Vineet Kumar, Ram Chandra
Maillard reactions products (MRPs) are a major colorant of distillery effluent. It is major source of environmental pollution due to its complex structure and recalcitrant nature. This study has revealed that sucrose glutamic acid-Maillard reaction products (SGA-MRPs) showed many absorption peaks between 200 and 450 nm. The absorption maximum peak was noted at 250 nm in spectrophotometric detection. This indicated the formation of variable molecular weight Maillard products during the SGA-MRPs formation at high temperature...
February 2, 2018: World Journal of Microbiology & Biotechnology
Rajni Hooda, Nishi K Bhardwaj, Pamela Singh
A gram positive, rod shaped, bacterium was isolated from pulp and paper mill sludge and characterized as Brevibacillus parabrevis (MTCC 12105) by biochemical tests and 16S rRNA gene sequencing. CD and EOP stage wastewater, collected from a leading pulp and paper mill situated in North India, was used for degradation study. Wastewater degradation efficiency of the bacterial isolate was evaluated by Flask study in batch mode and Reactor study in semi continuous mode. The isolated bacterium showed a considerable reduction of the colour (59%), chemical oxygen demand (62%) and lignin (53...
January 29, 2018: World Journal of Microbiology & Biotechnology
Anna Yang, Dongmei Tang, Xiulong Jin, Lin Lu, Xiaohong Li, Kun Liu
Arbuscular mycorrhizal (AM) fungi are vital soil microbes that connect many individual plants into a large functional organism via a vast mycelial network under the ground. In this study, the changes of soil AM fungal community in response to road-building disturbance caused by tourism development in Huangshan (Yellow Mountain) Scenic Area are assessed. Road building have brought negative effects on AM fungal community, inducing lower diversity parameters, including species number, spore density and diversity indices...
January 22, 2018: World Journal of Microbiology & Biotechnology
Xu Tang, Qiuli Ouyang, Guoxing Jing, Xingfeng Shao, Nengguo Tao
This study investigated the potential anti-fungal mechanisms of sodium dehydroacetate (SD) against Geotrichum citri-aurantii. The results showed that the cell wall integrity of G. citri-aurantii was not affected, whereas the membrane permeability of G. citri-aurantii mycelia was visibly altered by SD. Dramatic morphological changes of the mycelia, such as loss of cytoplasm, plasmolysis, and dissolution of intracellular substances, were observed by scanning electron microscopy and transmission electron microscopy analyses, indicating that the mycelium is severely damaged by the SD treatment...
January 19, 2018: World Journal of Microbiology & Biotechnology
Jeny Adina Larrea-Murrell, Marcia Maria Rojas-Badia, Ivette García-Soto, Beatriz Romeu-Alvarez, Tristano Bacchetti, Annika Gillis, Ana Karina Boltes-Espinola, Mayra Heydrich-Perez, Daysi Lugo-Moya, Jacques Mahillon
Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis...
January 19, 2018: World Journal of Microbiology & Biotechnology
Maria Marsova, Serikbay Abilev, Elena Poluektova, Valeriy Danilenko
Oxidative stress cause serious damages in human organism resulting in multiple diseases. Antioxidant therapy includes diet, the use of chemical agents or commensal bacteria such as lactobacilli. This study aims to evaluate the antioxidant (AO) activity of cell-free culture supernatants of lactobacilli, isolated from different parts of the human body. A test system based on Escherichia coli MG1655 strains carrying plasmids encoding luminescent biosensors pSoxS-lux and pKatG-lux inducible by superoxide anion and hydrogen peroxide, respectively, was used to analyze cell-free culture supernatants of lactobacilli...
January 17, 2018: World Journal of Microbiology & Biotechnology
Yu Su, Zhongkang Wang, Changwen Shao, Yuanli Luo, Li Wang, Youping Yin
Targeted gene disruption via Agrobacterium tumefaciens-mediated transformation (ATMT) and homologous recombination is the most common method used to identify and investigate the functions of genes in fungi. However, the gene disruption efficiency of this method is low due to ectopic integration. In this study, a high-efficiency gene disruption strategy based on ATMT and the split-marker method was developed for use in Nomuraea rileyi. The β-glucuronidase (gus) gene was used as a negative selection marker to facilitate the screening of putative transformants...
January 16, 2018: World Journal of Microbiology & Biotechnology
Tingting Liu, Fei Luo, Zhenyao Wang, Yuqin Li
Brewery effluent (BE) as an appreciable and sustainable resource presented new possibilities in low-cost algal biomass production, whereas the relatively low essential macronutrients hindered extensive applications as growth medium for microalgae cultivation. The objective of this study was to investigate the feasibility of an integrated treatment strategy initiated by BE coupling phytohormones in augmenting biomass and lipid accumulation in Coccomyxa subellipsoidea. Results revealed that BE coupling synthetic 1-naphthaleneacetic acid (NAA) accomplished the favorable lipid productivity of 481...
January 12, 2018: World Journal of Microbiology & Biotechnology
Markus Buchhaupt, Karin Lintz, Sonja Hüttmann, Jens Schrader
The culture supernatant of Caldariomyces fumago strains grown in a minimal medium with fructose contains mainly the biotechnologically relevant enzyme chloroperoxidase (CPO) and only minor amounts of other proteins. Our approach to identify the nature of these proteins via peptide mass fingerprinting and transcriptome analysis demonstrated the presence of putative glycosyl hydrolase and glucose oxidase (GOx) enzymes. These activities had been described earlier as parts of the fungus´ halogenation machinery, as they provide CPO with the co-substrate H2O2...
January 10, 2018: World Journal of Microbiology & Biotechnology
Magdalena Wypij, Joanna Czarnecka, Magdalena Świecimska, Hanna Dahm, Mahendra Rai, Patrycja Golinska
We report synthesis of silver nanoparticles (AgNPs) from Streptomyces xinghaiensis OF1 strain, which were characterised by UV-Vis and Fourier transform infrared spectroscopy, Zeta sizer, Nano tracking analyser, and Transmission electron microscopy. The antimicrobial activity of AgNPs alone, and in combination with antibiotics was evaluated against bacteria, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis, and yeasts viz., Candida albicans and Malassezia furfur by using micro-dilution method...
January 5, 2018: World Journal of Microbiology & Biotechnology
Zhiqiang Li, Hui Zhang, Jinliang Zhang, Li Xi, Guangli Yang, Shuli Wang, Qingfeng Zhou, Xiaogen Zhang, Junbo Zhang
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins...
January 4, 2018: World Journal of Microbiology & Biotechnology
Barbora Stratilová, Jaroslav Klaudiny, Pavel Řehulka, Eva Stratilová, Csilla Mészárosová, Soňa Garajová, Barbora Pavlatovská, Helena Řehulková, Stanislav Kozmon, Sergej Šesták, Zuzana Firáková, Renáta Vadkertiová
α-Galactosidases are assigned to the class of hydrolases and the subclass of glycoside hydrolases (GHs). They belong to six GH families and include the only characterized α-galactosidases from yeasts (GH 27, Saccharomyces cerevisiae). The present study focuses on an investigation of the lactose-inducible α-galactosidase produced by Papiliotrema flavescens. The enzyme was present on the surface of cells and in the cytosol. Its temperature optimum was about 60 °C and the pH optimum was 4.8; the pH stability ranged from 3...
January 4, 2018: World Journal of Microbiology & Biotechnology
T M Luong, O N Ponamoreva, I A Nechaeva, K V Petrikov, Ya A Delegan, A K Surin, D Linklater, A E Filonov
Production of trehalolipid biosurfactants by Rhodococcus erythropolis S67 depending on the growth temperature was studied. R. erythropolis S67 produced glycolipid biosurfactants such as 2,3,4-succinoyl-octanoyl-decanoyl-2'-decanoyl trehalose and 2,3,4-succinoyl-dioctanoyl-2'-decanoyl trehalose during the growth in n-hexadecane medium at 26 and 10 °C, despite the different aggregate state of the hydrophobic substrate at low temperature. The surface tension of culture medium was found being reduced from 72 to 27 and 45 mN m-1, respectively...
January 4, 2018: World Journal of Microbiology & Biotechnology
Haijiao Yan, Jianmin Yun, Duiyuan Ai, Wenwei Zhang, Jie Bai, Juan Guo
Public concern for food safety and environmental issues and the increase in fungicide-resistant pathogen have enhanced the interest in developing alternative methods to fungicides to control postharvest fruit decay. In this study, a bacterial strain isolated from stale potato vermicelli was identified as Bacillus pumilus HN-10 based on morphological characteristics and 16S rRNA gene sequence analysis. Furthermore, two novel cationic antifungal peptides named P-1 and P-2 were purified from B. pumilus HN-10 using macroporous adsorbent resin AB-8, Sephadex G-100 chromatography, and reversed-phase high-performance liquid chromatography...
January 4, 2018: World Journal of Microbiology & Biotechnology
Susana M Calle-Castañeda, Marco A Márquez-Godoy, Juan P Hernández-Ortiz
Phosphorus is an essential nutrient for the synthesis of biomolecules and is particularly important in agriculture, as soils must be constantly supplemented with its inorganic form to ensure high yields and productivity. In this paper, we propose a process to solubilize phosphorus from phosphate rocks, where Acidithiobacillus thiooxidans cultures are pre-cultivated to foster the acidic conditions for bioleaching-two-step "growing-then-recovery"-. Our method solubilizes 100% of phosphorus, whereas the traditional process without pre-cultivation-single-step "growing-and-recovery"-results in a maximum of 56% solubilization...
December 29, 2017: World Journal of Microbiology & Biotechnology
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