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World Journal of Microbiology & Biotechnology

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https://www.readbyqxmd.com/read/28550527/environmental-yeast-communities-in-vineyards-in-the-mountains-of-santa-catarina-state-brazil
#1
S D C Mendes, M Ramírez-Castrillón, N P Feldberg, F C Bertoldi, P Valente
Yeasts were isolated from three vineyards located in the South Region of Brazil. A cross evaluation was carried out at the oldest vineyard of the study in Pinheiro Preto. Samples of grape berries, grapevine leaves and the soil, along with samples of the winery equipment and effluent, were collected. In the Serra do Marari and Campos Novos vineyards only samples of grape clusters were obtained. The 106 yeast isolates were identified by sequencing the D1/D2 domain of LSU rDNA or ITS1-5.8S-ITS2 region in 22 species...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28547728/the-protective-effect-of-a-novel-antioxidant-gene-from-mycobacterium-avium-against-nitrosative-and-oxidative-stress-in-e-coli
#2
Shiyi He, Aiyan Wang, Xueping Ning, Dongjun Yang, Min Ling
The production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) is an important host defense mechanism in response to infection by Mycobacterium tuberculosis. A variety of genes have been implicated in resistance to ROI and RNI, including noxR1. However, studies in Mycobacterium avium, an important pathogen among nontuberculous mycobacteria, are limited. We aim to investigate the role of a novel gene cloned from M. avium with high similarity to noxR1, noA, in resistance against RNI and ROI in M...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28547727/isolation-purification-and-chemical-characterization-of-a-new-angucyclinone-compound-produced-by-a-new-halotolerant-nocardiopsis-sp-hr-4-strain
#3
Yamina Hadj Rabia-Boukhalfa, Yannick Eveno, Solange Karama, Okba Selama, Béatrice Lauga, Robert Duran, Hocine Hacène, Véronique Eparvier
A halotolerant Actinobacteria strain HR-4 was isolated from a salt lake soil sample in Algerian Sahara. Analysis of 16S rDNA gene sequence showed that strain HR-4 belonged to the genus Nocardiopsis. The similarity level ranges between 97.45 and 99.20% with Nocardiopsis species and Nocardiopsis rosea being the most closely related one. Morphological, physiological and phylogenetic characteristics comparisons showed significant differences with the nearest species. These data strongly suggest that strain HR-4 represents novel species...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28540631/efficient-environmentally-friendly-and-specific-valorization-of-lignin-promising-role-of-non-radical-lignolytic-enzymes
#4
REVIEW
Wenya Wang, Chao Zhang, Xinxiao Sun, Sisi Su, Qiang Li, Robert J Linhardt
Lignin is the second most abundant bio-resource in nature. It is increasingly important to convert lignin into high value-added chemicals to accelerate the development of the lignocellulose biorefinery. Over the past several decades, physical and chemical methods have been widely explored to degrade lignin and convert it into valuable chemicals. Unfortunately, these developments have lagged because of several difficulties, of which high energy consumption and non-specific cleavage of chemical bonds in lignin remain the greatest challenges...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28534113/cysteine-rich-low-molecular-weight-antimicrobial-peptides-from-brevibacillus-and-related-genera-for-biotechnological-applications
#5
REVIEW
Piyush Baindara, Anoop Kapoor, Suresh Korpole, Vishakha Grover
The production of natural antimicrobial peptides (AMPs) is an innate immunity trait of all life forms including eukaryotes and prokaryotes. While these AMPs are usually called as defensins in eukaryotes, they are known as bacteriocins in prokaryotes. Bacteriocins are more diverse AMPs considering their varied composition and posttranslational modifications. Accordingly, this review is focused on cysteine-rich AMPs resembling eukaryotic defensins such as laterosporulin from Brevibacillus spp. and associated peptides secreted by the members of related genera...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28534112/decolorization-of-textile-dye-rb19-using-volcanic-rock-matrix-immobilized-bacillus-thuringiensis-cells-with-surface-displayed-laccase
#6
Juan Wan, Xiaowen Sun, Cheng Liu, Mengjun Tang, Lin Li, Hong Ni
A triplicate volcanic rock matrix-Bacillus thuringiensis-laccase WlacD (VRMs-Bt-WlacD) dye decolorization system was developed. WlacD was displayed on the B. thuringiensis MB174 cell surface to prepare a whole-cell laccase biocatalyst by using two repeat N-terminal domains of autolysin Mbg (Mbgn)2 as the anchoring motif. Immunofluorescence microscopic assays confirmed that the fusion protein (Mbgn)2-WlacD was anchored on the surface of the recombinant B. thuringiensis MB174. After optimization by a single factor test, L 9(3(4))-orthogonal test, Plackett-Burman test, steepest ascent method, and Box-Behnken response surface methodology, the whole-cell specific laccase activity of B...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28534111/overexpression-of-ppc-or-deletion-of-mdh-for-improving-production-of-%C3%AE-aminobutyric-acid-in-recombinant-corynebacterium-glutamicum
#7
Feng Shi, Ming Zhang, Yongfu Li
L-Glutamate decarboxylase (GAD) transforms L-glutamate into γ-aminobutyric acid (GABA). Corynebacterium glutamicum that expresses exogenous GAD gene(s) can synthesize GABA from its own produced L-glutamate. To enhance GABA production in recombinant C. glutamicum strain SH, metabolic engineering strategies were used to improve the supply of the GABA precursor, L-glutamate. Five new strains were constructed here. First, the ppc gene was coexpressed with two GAD genes (gadB1 and gadB2). Then, the mdh gene was deleted in C...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28523623/microbial-population-shift-caused-by-sulfamethoxazole-in-engineered-soil-aquifer-treatment-e-sat-system
#8
Ashwinkumar P Rudrashetti, Niti B Jadeja, Deepa Gandhi, Asha A Juwarkar, Abhinav Sharma, Atya Kapley, R A Pandey
The engineered-Soil Aquifer Treatment (e-SAT) system was exploited for the biological degradation of Sulfamethoxazole (SMX) which is known to bio-accumulate in the environment. The fate of SMX in soil column was studied through laboratory simulation for a period of 90 days. About 20 ppm SMX concentration could be removed in four consecutive cycles in e-SAT. To understand the microbial community change and biological degradation of SMX in e-SAT system, metagenomic analysis was performed for the soil samples before (A-EBD) and after SMX exposure (B-EBD) in the e-SAT...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28500426/identification-and-evaluation-of-potential-bio-control-fungal-endophytes-against-ustilagonoidea-virens-on-rice-plants
#9
Mebeaselassie Andargie, Zhu Congyi, Yun Yun, Jianxiong Li
False smut disease of rice is posing an increasing concern for production, not only because of the hiking epidemic occurrence in rice production, but also because of the challenging specific pathogenesis of the disease. The aim of this work was to evaluate the potential of five fungal endophytes to reduce negative effects of rice false smut fungus (Ustilagonoidea virens) on rice plants, in both the laboratory and greenhouse. Though all the fungal isolates showed the ability to inhibit the growth of U. virens with varying degrees, isolate E337 showed significant antagonistic activity against the pathogenic fungi...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28497389/elucidating-and-engineering-thiopeptide-biosynthesis
#10
REVIEW
Philip R Bennallack, Joel S Griffitts
Initially discovered in the mid-twentieth century, thiopeptides constitute a diverse family of bacterially produced natural products exhibiting a remarkable array of biological properties. Only in the last several years have the details of thiopeptide biosynthesis been uncovered by a combination of genomic, genetic, and biochemical approaches. Thiopeptides are now known to be ribosomally synthesized and subsequently densely modified to carry azol(in)es, dehydro amino acids, and various other pathway-specific decorations...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28493157/determination-of-the-biofilm-production-capacities-and-characteristics-of-members-belonging-to-bacillaceae-family
#11
Arzu Coleri Cihan, Basar Karaca, Beste Piril Ozel, Tugba Kilic
The biofilm characteristics of many endospore-forming bacilli, especially the thermophiles are still unclear. In this study, a detailed identification and description of biofilm production characteristics of totally 145 isolates and reference strains belonging to Bacillaceae family, displaying thermophilic (n = 115), facultative thermophilic (n = 24) and mesophilic (n = 6) growth from genera Anoxybacillus, Geobacillus, Thermolongibacillus, Aeribacillus, Brevibacillus, Paenibacillus and Bacillus were presented...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28493156/mini-review-high-rate-algal-ponds-flexible-systems-for-sustainable-wastewater-treatment
#12
REVIEW
P Young, M Taylor, H J Fallowfield
Over the last 20 years, there has been a growing requirement by governments around the world for organisations to adopt more sustainable practices. Wastewater treatment is no exception, with many currently used systems requiring large capital investment, land area and power consumption. High rate algal ponds offer a sustainable, efficient and lower cost option to the systems currently in use. They are shallow, mixed lagoon based systems, which aim to maximise wastewater treatment by creating optimal conditions for algal growth and oxygen production-the key processes which remove nitrogen and organic waste in HRAP systems...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28488199/optimization-of-the-extraction-and-purification-of-the-compatible-solute-ectoine-from-halomonas-elongate-in-the-laboratory-experiment-of-a-commercial-production-project
#13
Ruifeng Chen, Lijun Zhu, Lihuo Lv, Su Yao, Bin Li, Junqing Qian
Optimization of compatible solutes (ectoine) extraction and purification from Halomonas elongata cell fermentation had been investigated in the laboratory tests of a large scale commercial production project. After culturing H. elongata cells in developed medium at 28 °C for 23-30 h, we obtained an average yield and biomass of ectoine for 15.9 g/L and 92.9 (OD600), respectively. Cell lysis was performed with acid treatment at moderate high temperature (60-70 °C). The downstream processing operations were designed to be as follows: filtration, desalination, cation exchange, extraction of crude product and three times of refining...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28488198/hydrogen-production-profiles-using-furans-in-microbial-electrolysis-cells
#14
Tunc Catal, Tansu Gover, Bugra Yaman, Jessica Droguetti, Kaan Yilancioglu
Microbial electrochemical cells including microbial fuel cells (MFCs) and microbial electrolysis cells (MECs) are novel biotechnological tools that can convert organic substances in wastewater or biomass into electricity or hydrogen. Electroactive microbial biofilms used in this technology have ability to transfer electrons from organic compounds to anodes. Evaluation of biofilm formation on anode is crucial for enhancing our understanding of hydrogen generation in terms of substrate utilization by microorganisms...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28488197/efficient-yeast-cell-surface-display-of-an-endoglucanase-of-aspergillus-flavus-and-functional-characterization-of-the-whole-cell-enzyme
#15
Gang Gao, Run-Qian Mao, Yue Xiao, Jing Zhou, Yu-Huan Liu, Gang Li
The endoglucanase gene endo753 from Aspergillus flavus NRRL3357 strains was cloned, and the recombinant Endo753 was displayed on the cell surface of Saccharomyces cerevisiae EBY100 strain by the C-terminal fusion using Aga2p protein as anchor attachment tag. The results of indirect immunofluorescence and Western blot confirmed the expression and localization of Endo753 on the yeast cell surface. The hydrolytic activity test of the whole-cell enzyme revealed that Endo753 immobilized on the yeast cell surface had high endoglucanase activity...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28470426/the-yeasts-phosphorelay-systems-a-comparative-view
#16
REVIEW
Griselda Salas-Delgado, Laura Ongay-Larios, Laura Kawasaki-Watanabe, Imelda López-Villaseñor, Roberto Coria
Cells contain signal transduction pathways that mediate communication between the extracellular environment and the cell interior. These pathways control transcriptional programs and posttranscriptional processes that modify cell metabolism in order to maintain homeostasis. One type of these signal transduction systems are the so-called Two Component Systems (TCS), which conduct the transfer of phosphate groups between specific and conserved histidine and aspartate residues present in at least two proteins; the first protein is a sensor kinase which autophosphorylates a histidine residue in response to a stimulus, this phosphate is then transferred to an aspartic residue located in a response regulator protein...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28470425/radiation-resistance-in-thermophiles-mechanisms-and-applications
#17
REVIEW
Preeti Ranawat, Seema Rawat
The study of prokaryotic life in high temperature environments viz., geothermal areas, hot, acidic geysers and undersea hydrothermal vents has revealed the existence of thermophiles (or hyperthermophiles). These microorganisms possess various stress adaptation mechanisms which enable them to bypass multiple physical and chemical barriers for survival. The discovery of radiation resistant thermophile Deinococcus geothermalis has given new insights into the field of radiation microbiology. The ability of radiation resistant thermophiles to deal with the lethal effects of ionizing radiations like DNA damage, oxidative bursts and protein damage has made them a model system for exobiology and interplanetary transmission of life...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28470424/nitrogen-transformation-under-different-dissolved-oxygen-levels-by-the-anoxygenic-phototrophic-bacterium-marichromatium-gracile
#18
Xuan Hong, Zhongwei Chen, Chungui Zhao, Suping Yang
Marichromatium gracile: YL28 (M. gracile YL28) is an anoxygenic phototrophic bacterial strain that utilizes ammonia, nitrate, or nitrite as its sole nitrogen source during growth. In this study, we investigated the removal and transformation of ammonium, nitrate, and nitrite by M. gracile YL28 grown in a combinatorial culture system of sodium acetate-ammonium, sodium acetate-nitrate and sodium acetate-nitrite in response to different initial dissolved oxygen (DO) levels. In the sodium acetate-ammonium system under aerobic conditions (initial DO = 7...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28466306/lactic-acid-bacteria-population-dynamics-during-spontaneous-fermentation-of-radish-raphanus-sativus-l-roots-in-brine
#19
Eleni Pardali, Spiros Paramithiotis, Marina Papadelli, Marios Mataragas, Eleftherios H Drosinos
The aim of the present study was to assess the microecosystem development and the dynamics of the lactic acid bacteria population during spontaneous fermentation of radish (Raphanus sativus L.) roots in brine at 20 and 30 °C. In both temperatures, lactic acid bacteria prevailed the fermentation; as a result, the pH value was reduced to ca. 3.6 and total titrable acidity increased to ca. 0.4% lactic acid. Enterococci population increased and formed a secondary microbiota while pseudomonads, Enterobacteriaceae and yeasts/molds populations were below enumeration limit already before the middle of fermentation...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28466305/concomitant-production-of-cellulase-and-xylanase-by-thermophilic-mould-sporotrichum-thermophile-in-solid-state-fermentation-and-their-applicability-in-bread-making
#20
Anju Bala, Bijender Singh
Sporotrichum thermophile BJAMDU5 secreted high titres of xylanolytic and cellulolytic enzymes in solid state fermentation using mixture of wheat straw and cotton oil cake (ratio 1:1) at 45 °C, pH 5.0 after 72 h inoculated with 2.9 × 10(7) CFU/mL conidiospores. Supplementation of solid medium with lactose and ammonium sulphate further enhanced the production of hydrolytic enzymes. Among different surfactants studied, Tween 80 enhanced the production of all enzymes [3455 U/g DMR (dry mouldy residue), 879...
June 2017: World Journal of Microbiology & Biotechnology
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