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Journal of Veterinary Diagnostic Investigation

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https://www.readbyqxmd.com/read/29973111/multiple-episodes-of-1080-sodium-monofluoroacetate-intoxication-in-a-california-calf-raising-operation
#1
John M Adaska, Guillermo Rimoldi, Patricia C Blanchard, John Champagne, Robert H Poppenga, Michelle Mostrom
Over a 1-y period, a California calf-raising operation experienced 4 separate episodes of sudden death in 4-6-mo-old steers. Each episode occurred in 1-3 contiguous pens on 1 of 3 properties owned by the operation, but eventually all 3 properties were involved. In each episode, animals appeared normal at the evening feeding but at the subsequent morning feeding were found dead or dying. Remaining live calves had a stiff gait and were often dribbling urine, but did not show respiratory signs until they were down and agonal...
July 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29952726/carcinoma-in-situ-within-an-area-of-barrett-esophagus-in-a-dog-with-megaesophagus
#2
Matthew A Kopke, John S Munday, Arnon Gal
A 10-y-old Irish Setter was presented with a history of recurrent episodes of regurgitation and vomiting, with more recent development of tachypnea. Megaesophagus had been diagnosed in the dog 2 y prior to this presentation. A solitary polypoid mass present immediately rostral to the lower esophageal sphincter was biopsied during percutaneous endoscopic gastrostomy tube placement. Barrett esophagus was diagnosed based on the observation of a polypoid mass with intestinal metaplasia that arose from the surrounding esophagus...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29938600/a-p37-based-elisa-used-to-monitor-anti-mycoplasma-hyorhinis-igg-in-serum-from-pigs-immunized-with-inactivated-m-hyorhinis-vaccines
#3
Eric A Bumgardner, Russell F Bey, Paulraj K Lawrence
Mycoplasma hyorhinis is an important pathogen of swine that can often occur as a respiratory coinfection with viral pathogens, but can also cause arthritis and polyserositis in infected animals. To date, no assay is available to assess the serologic response to M. hyorhinis vaccines, to our knowledge. We used recombinantly expressed M. hyorhinis p37 protein to monitor the magnitude of the IgG response in vaccinated animals. The assay was able to distinguish animals vaccinated with M. hyorhinis from those vaccinated with the other important Mycoplasma species: M...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29916768/an-improved-rapid-competitive-elisa-using-a-novel-conserved-3b-epitope-for-the-detection-of-serum-antibodies-to-foot-and-mouth-disease-virus
#4
Chungwon J Chung, Alfonso Clavijo, Mangkey A Bounpheng, Sabena Uddowla, Abu Sayed, Brooke Dancho, Ian C Olesen, Juan Pacheco, Barbara J Kamicker, David A Brake, Carey L Bandaranayaka-Mudiyanselage, Stephen S Lee, Devendra K Rai, Elizabeth Rieder
The highly contagious foot-and-mouth disease virus (FMDV) afflicts cloven-hoofed animals, resulting in significant costs because of loss of trade and recovery from disease. We developed a sensitive, specific, and rapid competitive ELISA (cELISA) to detect serum antibodies to FMDV. The cELISA utilized a monoclonal blocking antibody specific for a highly conserved FMDV nonstructural 3B epitope, a recombinant mutant FMDV 3ABC coating protein, and optimized format variables including serum incubation for 90 min at 20-25°C...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29888679/toxicity-associated-with-ingestion-of-a-polyacrylic-acid-hydrogel-dog-pad
#5
David C Dorman, Melanie L Foster, Brooke Olesnevich, Brad Bolon, Aude Castel, Marina Sokolsky-Papkov, Christopher L Mariani
Superabsorbent sodium polyacrylate polymeric hydrogels that retain large amounts of liquids are used in disposable diapers, sanitary napkins, and other applications. These polymers are generally considered "nontoxic" with acute oral median lethal doses (LD50 ) >5 g/kg. Despite this favorable toxicity profile, we identified a novel toxic syndrome in dogs and rats following the ingestion of a commercial dog pad composed primarily of a polyacrylic acid hydrogel. Inappropriate mentation, cerebellar ataxia, vomiting, and intention tremors were observed within 24 h after the ingestion of up to 15...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29877147/cellulitis-caused-by-the-burkholderia-cepacia-complex-associated-with-contaminated-chlorhexidine-2-scrub-in-five-domestic-cats
#6
Jessica K Wong, Lara C Chambers, Elizabeth J Elsmo, Tiffany L Jenkins, Elizabeth W Howerth, Susan Sánchez, Kaori Sakamoto
Isolates of the Burkholderia cepacia complex (BCC) are known as plant and human pathogens. We describe herein BCC infections as the cause of subcutaneous abscesses and purulent cellulitis in 5 cats. All cats were presented with an open wound, and 4 received standard wound care and empiric antibiotic therapy. Despite treatment, clinical signs worsened in 4 cats. Isolates of the BCC were obtained from all 5 cases. Two cats were submitted for postmortem examination. Subcutaneous abscesses with draining fistulas were observed...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29874973/bovine-pancreatolithiasis-case-report-and-review-of-the-literature
#7
Alfonso López, Shawn McKenna
An 8-y-old lactating Holstein cow was presented to the herd veterinarian with a history of acute ataxia, and because of poor prognosis, the animal was euthanized and submitted for postmortem examination. A lumbar vertebra was incompletely fractured and had an adjacent subdural hematoma. The pancreas had a hard texture with exaggerated nodular appearance, and on cut surface, the pancreatic ducts appeared fibrotic and contained scores of round-to-faceted pancreatoliths of 0.5-2.0 mm diameter. These calculi extended from the major ducts to the pancreatic duct, sphincter, and into the duodenal lumen...
June 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29790450/opisthotonos-and-unilateral-internal-hydrocephalus-associated-with-aberrant-migration-of-serratospiculum-sp-or-serratospiculoides-sp-in-a-prairie-falcon
#8
Arnaud J Van Wettere, Jaqueline P Kurz, Amanda Wilhelm, Jay Dee Ipsen
A juvenile, wild-caught prairie falcon ( Falco mexicanus) kept for falconry was presented to a veterinary hospital for intermittent opisthotonos and torticollis. Clinical examination, complete blood count, serum biochemistry panel, and fecal analysis were unremarkable. Clinical signs did not resolve, and the bird was euthanized 6 mo after the appearance of clinical signs. Autopsy revealed a mild, unilateral hydrocephalus and nematodes within the thoracic air sac. Histopathology demonstrated mild, unilateral hydrocephalus; scattered glial nodules; meningeal nematode sections; and meningeal and intraventricular embryonated eggs...
May 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29730973/development-of-in-situ-hybridization-for-detection-of-elephant-endotheliotropic-herpesvirus-in-asian-elephants
#9
Varankpicha Kochakul, Kittikorn Boonsri, Saruda Tiwananthagorn, Chalermchart Somgird, Chatchote Thitaram, Kidsadagon Pringproa
Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants ( Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling...
May 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29952744/diagnosis-of-virulent-newcastle-disease-in-southern-california-may-2018
#10
Francisco R Carvallo, Janet D Moore, Akinyi C Nyaoke, Linda Huang, Beate M Crossley, Francisco A Uzal
No abstract text is available yet for this article.
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29952743/corrigendum
#11
(no author information available yet)
Ong CB, Herdt TH, Fitzgerald SD. Hyperplastic goiter in two adult dairy cows. J Vet Diagn Invest 2014;26:810-814. (Original doi: 10.1177/1040638714554441). The use of mineral supplements as a top dressing and trace mineralized salt noted by the authors was based on non-documented verbal comments and not based on direct observation. Therefore, the definitive source of the excess iodine measured in the thyroid gland of the 2 cows examined was not identified. The acknowledgments for this case report have been revised to reflect appropriate contributors to the case...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29860939/sebaceous-cell-differentiation-in-a-canine-oral-papilloma
#12
Du-Gyeong Gang, Cheul-Hyeon Sim, Tae-Je Lee, Joo-Yeon Kong, Il-Hwa Hong
Papillomas caused by viral infection are well-known tumors in animals. Microscopic features typically include neoplastic epithelium with hyperkeratosis and koilocytes. An 8-y-old castrated male Shih Tzu dog was presented with a small exophytic mass on the external upper lip. The mass was diagnosed as a viral papilloma based on microscopic and immunohistochemical examination. Sebaceous cell differentiation was found in the neoplastic epithelium of the tumor, which is a rare finding in humans or animals.
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29860932/evaluation-of-a-duplex-reverse-transcription-real-time-pcr-assay-for-the-detection-of-encephalomyocarditis-virus
#13
Shaomin Qin, Darren Underwood, Luke Driver, Carol Kistler, Ibrahim Diallo, Peter D Kirkland
We evaluated a fluorogenic probe-based assay for the detection of encephalomyocarditis virus (EMCV) by comparing a set of published primers and probe to a new set of primers and probe. The published reagents failed to amplify a range of Australian isolates and an Italian reference strain of EMCV. In contrast, an assay based on 2 new sets of primers and probes that were run in a duplex reverse-transcription real-time PCR (RT-rtPCR) worked well, with high amplification efficiency. The analytical sensitivity was ~100-fold higher than virus isolation in cell culture...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29860931/gastrinoma-and-zollinger-ellison-syndrome-in-canids-a-literature-review-and-a-case-in-a-mexican-gray-wolf
#14
Jason D Struthers, Nick Robl, Valerie M Wong, Matti Kiupel
Gastrinoma, an infrequent diagnosis in middle-aged dogs, occurs with nonspecific gastrointestinal morbidity. Laboratory tests can yield a presumptive diagnosis, but definitive diagnosis depends on histopathology and immunohistochemistry. We describe a malignant pancreatic gastrinoma with lymph node metastases and corresponding Zollinger-Ellison syndrome in a Mexican gray wolf ( Canis lupus baileyi) and review this endocrine neoplasm in domestic dogs. A 12-y-old, captive, male Mexican gray wolf developed inappetence and weight loss...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29855226/disseminated-mycobacteriosis-caused-by-mycobacterium-kansasii-in-a-pot-bellied-pig
#15
Ryan Schafbuch, Stacy Tinkler, Chee Kin Lim, Rebecca Wolking, José Ramos-Vara
A 1.5-y-old spayed female Juliana pot-bellied pig was presented to the Purdue University Veterinary Teaching Hospital with a history of wasting and anorexia. Enlarged and partially mineralized lymph nodes were identified on radiographs and computed tomography scan. Generalized lymphadenomegaly and disseminated nodules in the lungs, liver, spleen, and kidneys were identified on postmortem examination. Histologic examination revealed caseonecrotic granulomas with numerous intracellular, acid-fast bacilli. Mycobacterium kansasii type II was identified as the etiologic agent by PCR amplification using universal Mycobacterium primers, direct sequencing of the PCR amplicon, and comparison to sequences in GenBank...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29790451/genital-mycoplasmas-of-healthy-bitches
#16
Zinka Maksimović, Alan Maksimović, Anis Halilbašić, Maid Rifatbegović
Little is known about the presence of mycoplasmas in the genital tracts of domestic and stray bitches or in the vaginas of ovariohysterectomized (OHE) bitches. Moreover, to our knowledge, there has been no research to investigate the presence of canine vaginal mycoplasmas during the different stages of the reproductive cycle. We investigated the occurrence of mycoplasmas in the vaginas of healthy domestic and stray intact bitches, to correlate their presence with specific stages of the reproductive cycle, and to compare them with those in OHE bitches...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29790449/pathology-of-coccidioidomycosis-in-llamas-and-alpacas
#17
Julian A Fernandez, Mireia N Hidalgo, Emir Hodzic, Santiago S Diab, Francisco A Uzal
Coccidioidomycosis is a fungal disease caused by either Coccidioides immitis or Coccidioides posadasii. Anecdotal evidence suggests that camelids are particularly susceptible to this disease and that a relatively large percentage of pneumonias in these animals are caused by Coccidioides spp. In a search of 21 y (1992-2013) of records from the California Animal Health and Food Safety Laboratory, we found 79 cases of coccidioidomycosis diagnosed in camelids; 66 (84%) had pneumonia and 13 (16%) had lesions only in organs other than the lungs...
July 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29701572/detection-and-phylogenetic-characterization-of-porcine-circovirus-2-from-pigs-in-mozambique
#18
Cláudio J Laisse, Carine K Souza, Paula R Pereira, Cíntia De Lorenzo, Matheus V Bianchi, Lourenço P Mapaco, Saulo P Pavarini, Cláudio W Canal, David Driemeier
Porcine circovirus-associated diseases (PCVADs), caused by porcine circovirus 2 (PCV-2), have a significant economic impact on the swine industry worldwide. In Africa, there is little information, to date, regarding the occurrence of PCV-2, and it has not been reported in Mozambique's swine population. We randomly collected mesenteric lymph nodes ( n = 111) from slaughtered pigs from 9 districts in southern Mozambique. PCV-2 DNA was detected in 54% (62 of 111) of the samples and 78% (23 of 31) of the farms...
May 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29601777/a-comparison-of-manual-counting-of-rabbit-reticulocytes-with-advia-2120i-analyzer-counting
#19
Annemarie E Kaufhold, Johannes Hirschberger, Sven Reese, Gesine Foerster, Jutta Hein
We compared manual counting of reticulocytes in rabbits with automatic counting using an ADVIA 2120i analyzer. Reproducibility and the influence of different anticoagulants (EDTA and Li-heparin) were also examined. Blood samples of 331 rabbits (method comparison, n = 289; reproducibility, n = 33; comparison of anticoagulants, n = 9) were tested. The reticulocyte numbers of each specimen were manually determined twice for method comparison. Passing-Bablok regressions, Bland-Altman plots, and the coefficient of variation (CV) were used to evaluate statistical significance...
May 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29528813/non-toxigenic-corynebacterium-ulcerans-sequence-types-325-and-339-isolated-from-two-dogs-with-ulcerative-lesions-in-italy
#20
Virginia Carfora, Fabia Scarampella, Manuela Iurescia, Valentina Donati, Fiorentino Stravino, Serena Lorenzetti, Erika Menichini, Alessia Franco, Andrea Caprioli, Antonio Battisti
Corynebacterium ulcerans, an emerging pathogen related to C. diphtheriae and C. pseudotuberculosis, is able to cause disease in both human and animal hosts. C. ulcerans may harbor acquired virulence factors such as dermonecrotic exotoxin phospholipase D (PLD) and the prophage-encoded diphtheria toxin (DT). Infections typically occur in persons reporting close contact with animals. In pets, C. ulcerans has been isolated from both asymptomatic carriers and clinically affected dogs and cats. We describe the isolation and characterization of C...
May 2018: Journal of Veterinary Diagnostic Investigation
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