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Journal of Veterinary Diagnostic Investigation

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https://www.readbyqxmd.com/read/29034814/entomophthoromycosis-and-mucormycosis-as-causes-of-pneumonia-in-vietnamese-potbellied-pigs
#1
Dawn E Evans, Atsushi Kawabata, Leslie D Wilson, Kenneth Kim, Shannon D Dehghanpir, Steve D Gaunt, Matt Welborn, Britton Grasperge, Marjorie S Gill
Two Vietnamese potbellied pigs ( Sus scrofa) had respiratory disease and, on autopsy, both pigs had large masses in the lungs and thoracic cavity. Microscopically, pulmonary and pleural masses contained large areas with hyphae surrounded by hypereosinophilic cellular debris rimmed by abundant eosinophils, lymphocytes, plasma cells, and histiocytes with occasional multinucleate giant cells. The hypereosinophilic debris usually formed tight cuffs, or "sleeves" around the hyphae, compatible with Splendore-Hoeppli-like material...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29034813/a-review-of-medical-terminology-standards-and-structured-reporting
#2
Abdullah Awaysheh, Jeffrey Wilcke, François Elvinger, Loren Rees, Weiguo Fan, Kurt Zimmerman
Much effort has been invested in standardizing medical terminology for representation of medical knowledge, storage in electronic medical records, retrieval, reuse for evidence-based decision making, and for efficient messaging between users. We only focus on those efforts related to the representation of clinical medical knowledge required for capturing diagnoses and findings from a wide range of general to specialty clinical perspectives (e.g., internists to pathologists). Standardized medical terminology and the usage of structured reporting have been shown to improve the usage of medical information in secondary activities, such as research, public health, and case studies...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29034785/treatment-history-and-antimicrobial-susceptibility-results-for-mannheimia-haemolytica-pasteurella-multocida-and-histophilus-somni-isolates-from-bovine-respiratory-disease-cases-submitted-to-the-iowa-state-university-veterinary-diagnostic-laboratory-from-2013
#3
Drew R Magstadt, Adlai M Schuler, Johann F Coetzee, Adam C Krull, Annette M O'Connor, Vickie L Cooper, Terry J Engelken
Bovine respiratory disease is the most costly disease facing the cattle industry. Increasing resistance to antimicrobial treatment has been presented as a significant contributing factor, often through summarized susceptibility testing data. We assessed the relationship between previous antimicrobial treatment and antimicrobial susceptibility results from isolates of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni cultured from bovine respiratory cases submitted to the Iowa State University Veterinary Diagnostic Laboratory from 2013 to 2015...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29020886/comparison-of-culture-methodology-for-the-detection-of-methicillin-resistant-staphylococcus-pseudintermedius-in-clinical-specimens-collected-from-dogs
#4
Matthew E Saab, C Anne Muckle, Henrik Stryhn, J Trenton McClure
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a major pathogen in dogs and has been implicated as a hospital-acquired pathogen in veterinary hospitals. We attempted to determine if selective culture methods will detect more MRSP when compared to the traditional culture methods in clinical samples from dogs in Atlantic Canada with a high risk for MRSP infection. Each sample was tested using 4 culture methods: traditional culture; mannitol salt agar with 2 μg/mL of oxacillin (MSAox); enrichment broth (EB) with MSAox; and EB with traditional culture...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29020879/immunohistochemical-and-quantitative-rt-pcr-methods-to-assess-rank-expression-in-normal-and-neoplastic-canine-mammary-gland
#5
Raquel Sánchez-Céspedes, Yolanda Millan, Silvia Guil-Luna, Jesús García-Macías, Lorella Maniscalco, Selina Iussich, Raffaella De Maria, Juana M de Las Mulas
The receptor activator of nuclear factor-kB ( RANK) gene is found in both human and murine mammary epithelial cells and in human cancer cell lines. We analyzed RANK expression in normal and proliferative canine mammary tissue samples ( n = 47) and cell lines ( n = 10), and identified its expression in epithelial cell populations. The correlation of RANK protein with clinicopathologic parameters was also studied. A double immunohistochemical method using RANK and p63 antibodies was applied to 33 tissue samples to analyze RANK protein expression and its possible co-expression with p63 protein, the latter used to identify myoepithelial (ME) cells (p63-positive) or luminal epithelial (LE) cells (p63-negative)...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28985710/early-life-exposures-and-johne-s-disease-risk-in-zoo-ruminants
#6
Tristan L Burgess, Carmel L Witte, Bruce A Rideout
Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic, progressive bacterial enteritis of ruminants that can cause serious losses in both livestock and exotic species. Infection risk in exotic ruminants is associated with maternal infection status, but the effect of other herdmates on risk of infection has not been reported, to our knowledge. We conducted a retrospective cohort study to evaluate the association between MAP infection status and early-life contact with infected herdmates...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28985709/detection-of-treponema-phagedenis-like-antibodies-in-serum-and-bulk-milk-from-cows-with-and-without-digital-dermatitis
#7
Jenny Frössling, Anna Rosander, Camilla Björkman, Katarina Näslund, Märit Pringle
Digital dermatitis (DD) in dairy cows is a widespread disease linked to infection with Treponema. The traditional diagnostic method is clinical inspection, which is subjective and laborious. We explored the performance of 4 different immunogenic proteins from Treponema phagedenis in a new antibody ELISA for analysis of serum or milk. Analysis of samples from 390 cows in 25 herds showed that the ELISA could distinguish the majority of cows with DD from healthy cows. By changing the cutoff and applying parallel or serial testing, high sensitivity or specificity could be achieved...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28985698/application-of-real-time-quantitative-pcr-assays-for-detecting-marine-brucella-spp-in-fish
#8
Stephanie A Norman, Martha A Delaney, Katherine H Haman, Austen C Thomas, Jacques Godfroid, Anett K Larsen, Ingebjørg H Nymo, Suelee Robbe-Austerman, Christine Quance, Jack C Rhyan, Dyanna M Lambourn, Steven J Jeffries, Peter Rabinowitz
Brucella ceti and Brucella pinnipedialis have been documented as occurring in marine mammals, and B. ceti has been identified in 3 naturally acquired human cases. Seroconversion and infection patterns in Pacific Northwest harbor seals ( Phoca vitulina richardii) and North Atlantic hooded seals ( Cystophora cristata) indicate post-weaning exposure through prey consumption or lungworm infection, suggesting fish and possibly invertebrates play an epizootiologic role in marine Brucella transmission and possible foodborne risk to humans...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28980519/exploring-target-specific-primer-extension-in-combination-with-a-bead-based-suspension-array-for-multiplexed-detection-and-typing-using-streptococcus-suis-as-a-model-pathogen
#9
Fimme J van der Wal, René P Achterberg, Conny van Solt-Smits, Jan H W Bergervoet, Marjanne de Weerdt, Henk J Wisselink
We investigated the feasibility of an assay based on target-specific primer extension, combined with a suspension array, for the multiplexed detection and typing of a veterinary pathogen in animal samples, using Streptococcus suis as a model pathogen. A procedure was established for simultaneous detection of 6 S. suis targets in pig tonsil samples (i.e., 4 genes associated with serotype 1, 2, 7, or 9, the generic S. suis glutamate dehydrogenase gene [ gdh], and the gene encoding the extracellular protein factor [ epf])...
October 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28971754/phylogroup-and-virulence-gene-association-with-clinical-characteristics-of-escherichia-coli-urinary-tract-infections-from-dogs-and-cats
#10
Tabitha A Hutton, Gabriel K Innes, Josée Harel, Philippe Garneau, Andrew Cucchiara, Dieter M Schifferli, Shelley C Rankin
Escherichia coli isolates from infections outside the gastrointestinal tract are termed extra-intestinal pathogenic E. coli (ExPEC) and can be divided into different subpathotypes; one of these is uropathogenic E. coli (UPEC). The frequency with which UPEC strains cause urinary tract infections in dogs and cats is not well documented. We used an oligonucleotide microarray to characterize 60 E. coli isolates associated with the urinary tract of dogs ( n = 45) and cats ( n = 15), collected from 2004 to 2007, into ExPEC and UPEC and to correlate results with patient clinical characteristics...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906208/pattern-recognition-receptors-signaling-pathways-and-dysregulation-in-canine-chronic-enteropathies-brief-review
#11
Romy M Heilmann, Karin Allenspach
Pattern-recognition receptors (PRRs) are expressed by innate immune cells and recognize pathogen-associated molecular patterns (PAMPs) as well as endogenous damage-associated molecular pattern (DAMP) molecules. With a large potential for synergism or convergence between their signaling pathways, PRRs orchestrate a complex interplay of cellular mediators and transcription factors, and thus play a central role in homeostasis and host defense. Aberrant activation of PRR signaling, mutations of the receptors and/or their downstream signaling molecules, and/or DAMP/PAMP complex-mediated receptor signaling can potentially lead to chronic auto-inflammatory diseases or development of cancer...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906183/odontomas-in-two-long-finned-ocellaris-clownfish-amphiprion-ocellaris
#12
Bryan S Vorbach, Jeffrey C Wolf, Roy P Yanong
Two adult long-finned ocellaris clownfish ( Amphiprion ocellaris) from 2 different aquaculture facilities were examined at the University of Florida, Tropical Aquaculture Laboratory for oral masses. Incisional biopsies were obtained from the masses under anesthesia, and histologic examination revealed both to be odontomas. Most benign odontomas are classified as hamartomas (disorganized proliferations of tissues found normally at the site of origin). Odontomas have previously been reported in wild teleost fish in association with viral infections and pollution...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906182/multi-locus-sequence-typing-of-streptococcus-equi-subspecies-zooepidemicus-strains-isolated-from-cats
#13
Ann P Britton, Shlomo E Blum, Carolyn Legge, Ken Sojonky, Erin N Zabek
Streptococcus equi subspecies zooepidemicus ( S. zooepidemicus) causes outbreaks of fatal respiratory disease in dog shelters and fatal respiratory and neurologic disease in cat shelters. We conducted multi-locus sequence typing analysis on S. zooepidemicus isolates from 5 Canadian and 3 Israeli cats with severe respiratory and neurologic disease, plus 1 isolate from a clinically normal shelter cat. Our aim was to determine if feline outbreaks are clonal and whether there is commonality between feline and canine strains...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906181/a-decade-of-using-small-to-medium-throughput-allele-discrimination-assay-to-determine-prion-protein-gene-prnp-genotypes-in-sheep-in-slovenia
#14
Jelka Zabavnik, Marko Cotman, Polona Juntes, Ivan Ambrozic
Sheep with valine (V) at codon 136 and glutamine (Q) at codon 171 of the prion protein gene ( Prnp) are highly susceptible to classical scrapie, whereas phenylalanine (F) at codon 141 and histidine (H) at codon 154 play a major role in the susceptibility to atypical scrapie. A TaqMan real-time PCR assay was developed to determine Prnp alleles at codons 136, 141, 154, and 171 and used in classical scrapie eradication and breeding programs adopted in Slovenia. The frequency of the most resistant genotypes ARR/ARR and ARR/ARQ increased significantly in tested animals ( n = 35,138) from 6...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906180/amplification-of-mycoplasma-haemofelis-dna-by-a-pcr-for-point-of-care-use
#15
Jennifer Hawley, Tal Yaaran, Sarah Maurice, Michael R Lappin
We compared a qualitative in-clinic (IC)-PCR for the detection of Mycoplasma haemofelis DNA with the results of a commercial qualitative laboratory-based, conventional (c)PCR. In order to determine the specificity of both tests, Bartonella spp. samples were included. Forty-three previously tested blood samples with known PCR results for hemoplasmas and Bartonella spp. were selected. The samples were split between 2 laboratories. At the first laboratory, DNA was purified and run on 2 cPCR assays for the detection of hemoplasmas and Bartonella spp...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906179/multi-locus-sequence-types-of-mycoplasma-bovis-isolated-from-ontario-canada-in-the-past-three-decades-have-a-temporal-distribution
#16
Patricia Bell-Rogers, Lois Parker, Hugh Y Cai
A total of 217 Mycoplasma bovis isolates cultured from clinical cases in Ontario, Canada, over the past 30 y were selected to be characterized by a multi-locus sequence typing (MLST) method. Eleven housekeeping genes were evaluated for suitability for MLST; 2 loci that had been used in prior MLST schemes, dnaN and metS, along with hsp70 were chosen for further sequence analysis. The remaining loci- adk, efp, gmk, gyrB, polC, rpoB, tpiA, and uvrC genes-were not used because they had little to no sequence variation...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906178/comparison-of-whole-genome-sequencing-to-restriction-endonuclease-analysis-and-gel-diffusion-precipitin-based-serotyping-of-pasteurella-multocida
#17
Karen J LeCount, Linda K Schlater, Tod Stuber, Suelee Robbe Austerman, Timothy S Frana, Ronald W Griffith, Matthew M Erdman
The gel diffusion precipitin test (GDPT) and restriction endonuclease analysis (REA) have commonly been used in the serotyping and genotyping of Pasteurella multocida. Whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis has become the gold standard for other organisms, offering higher resolution than previously available methods. We compared WGS to REA and GDPT on 163 isolates of P. multocida to determine if WGS produced more precise results. The isolates used represented the 16 reference serovars, isolates with REA profiles matching an attenuated fowl cholera vaccine strain, and isolates from 10 different animal species...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28906177/brachyspira-hyodysenteriae-detection-in-the-large-intestine-of-slaughtered-pigs
#18
Friederike Zeeh, Silvio De Luca, Pamela Nicholson, Niels Grützner, Christina Nathues, Vincent Perreten, Heiko Nathues
Detection of subclinical Brachyspira hyodysenteriae infection in pig herds using feces is challenging. However, the ability to detect the pathogen in intestinal samples of slaughtered pigs has not been investigated, to our knowledge. Therefore, we determined the detection of B. hyodysenteriae in the colon, cecum, and rectum from slaughtered pigs. We analyzed the correlation between detection rates and intestinal lesions, ingesta or fecal consistency, and time from sample collection until processing. A total of 400 ingesta-mucosal (colon, cecum) and 200 fecal (rectum) samples from 200 pigs originating from 20 different herds were bacteriologically examined using selective culture followed by Brachyspira spp...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28868985/use-of-two-commercial-caprine-arthritis-encephalitis-immunoenzymatic-assays-for-screening-of-arthritic-goats
#19
Michał Czopowicz, Olga Szaluś-Jordanow, Agata Moroz, Marcin Mickiewicz, Lucjan Witkowski, Iwona Markowska-Daniel, Emilia Bagnicka, Jarosław Kaba
Roughly one-fourth of goats infected with small ruminant lentivirus (SRLV) develop caprine arthritis-encephalitis (CAE). We compared the profile of antibody response to surface glycoprotein (SU), and combined transmembrane glycoprotein and capsid protein (TM/CA) in SRLV-infected arthritic and asymptomatic goats, and determined the ability of 2 commercial ELISAs to distinguish between arthritic and asymptomatic goats. We used sera from 312 SRLV-seropositive dairy goats in a whole-virus ELISA; 222 were collected from arthritic goats and 90 from apparently healthy goats...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28862083/detection-of-salmonella-spp-in-veterinary-samples-by-combining-selective-enrichment-and-real-time-pcr
#20
Laura B Goodman, Patrick L McDonough, Renee R Anderson, Rebecca J Franklin-Guild, James R Ryan, Gillian A Perkins, Anil J Thachil, Amy L Glaser, Belinda S Thompson
Rapid screening for enteric bacterial pathogens in clinical environments is essential for biosecurity. Salmonella found in veterinary hospitals, particularly Salmonella enterica serovar Dublin, can pose unique challenges for culture and testing because of its poor growth. Multiple Salmonella serovars including Dublin are emerging threats to public health given increasing prevalence and antimicrobial resistance. We adapted an automated food testing method to veterinary samples and evaluated the performance of the method in a variety of matrices including environmental samples ( n = 81), tissues ( n = 52), feces ( n = 148), and feed ( n = 29)...
August 1, 2017: Journal of Veterinary Diagnostic Investigation
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