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Animal Biotechnology

Sihuan Zhang, Haiyu Zhao, Chuzhao Lei, Chuanying Pan, Hong Chen, Qing Lin, Xianyong Lan
Paired-like homeodomain 2 (PITX2), a key gene in hypothalamic-pituitary-adrena axis, influences animal growth and development. The objective of this study was to identify the association of the functional genetic variations within goat PITX2 gene with growth traits and mRNA expression levels. According to the reported single nucleotide polymorphisms (SNPs) information in Guanzhong dairy goat (GZDG), we identified genotypes of the known SNPs in Hainan black goat (HNBG). Association analysis uncovered that the SNPs of AC_000163: g...
January 17, 2019: Animal Biotechnology
JianPing Li, Wei Chen, SuFang Wu, Tao Ma, HuaiZhi Jiang, QiaoLing Zhang
Melanocyte stimulating hormone receptor (MC1R) has been known as a regulator of eumelanin and phaeomelanin production in the melanocytes, and MC1R mutations causing coat color changes are known in many vertebrates; however, there are no research reports about the differentially expression of MC1R gene and its coding protein in Cashmere goats with different coat color. We examined the presence of MC1R distribution and MC1R protein and gene expression in the white Cashmere goats and black Cashmere goats, respectively; q-PCR, Western blot and immunhistochemical analysis showed that the expression of the MC1R gene in the black Cashmere goats was 3...
January 14, 2019: Animal Biotechnology
Jiugang Zhao, Xi Long, Yihuan Yang, Hongmei Pan, Liang Zhang, Zongyi Guo, Jinyong Wang, Jing Lan
The immunophilins are an important group of regulatory molecules in the immune system. FKBP5, expressed throughout mammals and in fish and birds, functions in both physiological and pathogenic pathways, including innate immunity and steroid-based diseases. In this study, we cloned the first porcine FKBP5 from Rongchang pig by the rapid amplification of cDNA ends technique. The full-length cDNA is 4097 bp, with an open reading frame of 1371 bp that codes for a 457-aa protein. Western blotting detected the porcine FKBP5 protein at highest levels in thymus, followed by spleen and lung...
January 14, 2019: Animal Biotechnology
Yuanyuan Zheng, Zeying Wang, Yubo Zhu, Wei Wang, Man Bai, Qian Jiao, Yanru Wang, Sujun Zhao, Xianbo Yin, Dan Guo, Wenlin Bai
Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200 nt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber...
January 11, 2019: Animal Biotechnology
Tong Zhou, Hancheng Wei, Dairui Li, Wenjing Yang, Yang Cui, Jiayang Gao, Ting Yu, Xiaoyan Lv, Chuanying Pan
Lysine demethylase 4D (KDM4D) encodes a histone demethylase, which can influence the androgen signaling as well as play an essential role in spermatogenesis. Recently a study has shown that the mRNA of KDM4D is the key to the successful clone of the Macaque monkeys named as "Zhongzhong" and "Huahua," which greatly increased pregnancy rates of female monkey, suggesting that the KDM4D gene may be strongly associated with reproduction. Therefore, the objective of this study was to explore possible single nucleotide polymorphisms (SNPs) within the coding region of KDM4D gene and analyze the associations with testis morphology traits of male pigs...
January 5, 2019: Animal Biotechnology
Kala K Jacob, G Radhika, T V Aravindakshan
Recognition of deleterious non-synonymous single nucleotide polymorphism (SNPs) aids in the assessment of genetic basis of diseases and prediction of clinical phenotypes. In this study, data obtained from whole exome sequencing of Vechur cow using Illumina HiSeq 2500 platform is compared with that of crossbred cattle of Kerala. Sequence analysis of selected 18 mastitis resistant genes, evaluated the consequence of non-synonymous SNPs in these genes from both Vechur and crossbred cattle of Kerala, using sequence and structure-based computational tools such as SIFT, PROVEAN and I-MUTANT 2...
January 5, 2019: Animal Biotechnology
Yan Wang, Xia Xiao, Linjie Wang
Skeletal muscle satellite cells (SMSCs) provide a good model for the study of postnatal muscle growth and intramuscular adipogenesis because they have myogenic and adipogenic differentiation ability. In this study, we harvested goat SMSCs using pronase digestion method. Then, the cells were purified from stratified liquids between 40 and 90% percoll. Multinucleated myotubes formed and the myogenic genes were expressed during the myogenic differentiation. PAX7 showed the highest expression level on the proliferating phase and decreased during differentiation...
January 2, 2019: Animal Biotechnology
Zengrong Zhang, Huarui Du, Chaowu Yang, Qingyun Li, Mohan Qiu, Xiaoyan Song, Chunlin Yu, Xiaoyu Jiang, Lan Liu, Chenming Hu, Bo Xia, Xia Xiong, Li Yang, Han Peng, Xiaosong Jiang
OBJECTIVE: The goal of this study was to investigate the mechanisms of muscle growth and development of three chicken breeds. PARTICIPANTS: Eighteen chickens, including three different breeds with different growth speeds (White Broiler, Daheng, and Commercial Layers of Roman), were used. METHODS: Total RNA from breast muscle of these chickens was subjected to a gene expression microarray. Differentially expressed genes (DEGs) were screened and functional enrichment analysis was performed using DAVID...
January 2, 2019: Animal Biotechnology
Alicja Mizera, Marian Kuczaj, Anna Szul, Jarosław Jędraszczyk
The present study was aimed to determine the effect of GnRH analog (buserelin acetate) on the quality of bovine spermatozoa stored at 16° C for 24 h. Semen collected in the summer season from June to September from healthy Polish Holstein-Friesian bulls. Ejaculates were centrifuged, divided and diluted to the final concentration of 240 × 106 spermatozoa/mL using animal protein-free commercial BIOXcell® extender (IMV Technologies, L'aigle, France) (Control) or with BIOXcell® extender supplemented with buserelin acetate and stored 0, 8 and 24 h...
December 31, 2018: Animal Biotechnology
Liping Zhao, Changping Ma, Huibin Song, Yue Feng, Yang Li, Xuanyan Xia, Jialian Li, Fenge Li
Spermatogenesis is a complex process regulated by many genes. In this study, H2AFZ, RNF4 and NR4A1 genes were selected as candidate genes for boar semen quality traits based on their functions during spermatogenesis, and the associations of three loci (H2AFZ c.192 + 210-192 + 213delCGAT, RNF4 c.374 + 358 T > C and NR4A1 c.956 + 796 A > G) with sperm quality traits were analyzed in Duroc (n = 185), Large White (n = 87) and Landrace (n = 49) pig populations. The results showed H2AFZ c...
December 31, 2018: Animal Biotechnology
Jiyao Wu, Jianjin Wang, Binglin Yue, Fang Xing-Tang, Chunlei Zhang, Yun Ma, Linyong Hu, Yueyu Bai, Xingshan Qi, Hong Chen
The objective of this study was to examine the association of transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) genes polymorphisms with growth traits in three Chinese cattle breeds (Jiaxian red cattle, Qinchuan cattle and Luxi cattle). Through experiments we identified three single nucleotide polymorphisms (SNPs) in these three cattle breeds TRPV1 and TRPA1 genes using PCR-SSCP, (forced) PCR-RFLP methods. Three of these polymorphisms are all synonymous mutation which includes (NW_003104493...
December 31, 2018: Animal Biotechnology
Qiuling Li, Jie Qiao, Zhengfeng Zhang, Xiaolan Shang, Zhuodong Chu, Yajuan Fu, Mingxing Chu
The mechanisms that dairy cattle respond to environmental stresses are very complicated. Previous research into the molecular mechanisms of mammalian heat stress has largely focused on the role of protein-coding genes and small non-coding RNAs. Recently, it has become apparent that large numbers of long non-coding RNAs transcribed from mammalian genomes play extensive roles in transcriptional regulation. However, the expression of lncRNAs and their biological functions in heat stress in dairy cattle remain unknown...
December 27, 2018: Animal Biotechnology
Swati Kumari, P K Ray, Rahul Singh, P A Desingu, G Taru Sharma, G Saikumar
The objective of the present study was to develop a rapid, simple, specific and sensitive Taqman-based real-time PCR assay for porcine sapelovirus (PSV) detection. Specific primers and probe were designed from the five untranslated regions (UTRs) of the viral genome. The detection limit of the real-time PCR was 102 copies. The specificity of the Taqman real-time PCR assay was evaluated using other animal viruses and nuclease free water as a negative control. Strong fluorescent signals were obtained only in the detection of PSV real-time PCR and conventional RT-PCR were preformed simultaneously on 90 faecal samples...
December 24, 2018: Animal Biotechnology
Mohamed I Husseiny, Sameh Soliman
Recombinant BK virus (rBKV) is able to express polypeptides under control of its native BKV late promoter. This ability helps to use this construct as a good reporter since it can infect human cells. In this study, we generate a BKV construct containing Renilla luciferase (Rluc) sequences under control of the BKV late promoter. The activity of Rluc was strongly detected in Vero-76 and Cos-1 cells transfected with rBKV-Rluc-myc-2A-VP2 construct, indicating the production of a functional enzyme driven by the native late promoter...
December 24, 2018: Animal Biotechnology
Yi-Fan Wen, Li Zheng, Hui Niu, Guo-Liang Zhang, Gui-Min Zhang, Yi-Lei Ma, Yi-Ran Tian, Yan-Rong Liu, Peng Yang, Di-Yi Yang, Chu-Zhao Lei, Rui-Hua Dang, Xing-Lei Qi, Hong Chen, Bi-Zhi Huang, Yong-Zhen Huang
Cellular retinoic acid binding protein 2 (CRABP2) is essential to myoblast differentiation. However, there was no report about the function of CRABP2 gene in cattle. This study explored the association of CRABP2 gene polymorphisms with growth traits in cattle breeds by several methods, such as DNA sequencing, PCR, PCR-RFLP and forced PCR-RFLP. Two sequence variants were determined. There were 621 individuals in six cattle breeds from China for the experiment, and three breeds were used to test validation of polymorphisms and extent of linkage disequilibrium (LD)...
December 20, 2018: Animal Biotechnology
Wanting Dong, Jie Yang, Qin Zhang, Li Jiang
Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) is among the many candidate genes for regulating milk production traits in dairy cattle that have been identified via quantitative trait locus (QTL) mapping and genome-wide association studies (GWAS). Our previous studies confirmed that a G-to-A mutation at chr14:2553998 is the main cause of GPIHBP1-related effects on milk fat content. In this study, we discovered that GPIHBP1 may be a strong candidate gene for the regulation of milk protein traits...
December 20, 2018: Animal Biotechnology
Yeondong Cho, Yoonki Heo, Hanul Choi, Ki Hoon Park, Sehyun Kim, Yuyeon Jang, Hee-Jung Lee, Minji Kim, Young Bong Kim
PERV is a major virus concerning xenotransplantation study. However, the interesting part is that PERV is present in all kinds of pigs without pathogenicity and immune response. Furthermore, since pig cells have receptors for PERV, the gene delivery system using PERV envelope is highly likely to develop into an excellent viral vector in pigs. We developed a recombinant baculovirus with a modified surface for expressing the porcine endogenous retrovirus (PERV) envelope. Porcine reproductive and respiratory syndrome virus (PRRSV) infection is a severe concern in the porcine industry due to reproduction failure and respiratory symptoms...
December 20, 2018: Animal Biotechnology
Deepak Agarwal, Pathakota Gireesh-Babu, Annam Pavan-Kumar, Prakash Koringa, Chaitanya G Joshi, Adnan Gora, Irfan Ahmad Bhat, Aparna Chaudhari
The 17-beta-hydroxysteroid dehydrogenase 2 (17β-HSD2) enzyme regulates steroid levels by the inactivation of estrogen and androgens. Spermatogenesis associated protein 2 (SPATA2) plays a vital role in spermatogenesis in vertebrates including fish. We report cloning and characterization of full cds of 17β-HSD2 and SPATA2 genes in Clarias magur. The full-length cDNA sequences of 17β-HSD2 and SPATA2 were 1187 bp (ORF 1125 bp) and 1806 bp (ORF 1524 bp) encoding 375 and 508 amino acids, respectively. Signal peptide analysis revealed SPATA2 is nonsecretory, while 17β-HSD2 is a secretory protein...
December 20, 2018: Animal Biotechnology
Qing Xu, Yong Wang, Jiangjiang Zhu, Yanying Zhao, Yaqiu Lin
GTP binding protein overexpressed in skeletal muscle (GEM) is an important gene with many functions, such as regulating the rearrangement of cytoskeleton and the activity of voltage-dependent calcium channel, and GEM was regarded as a candidate gene for obesity. However, little investigation has been carried out to explore whether GEM affected the intramuscular fat (IMF) deposition of goat. To explore the role of GEM gene in goat, this gene was cloned and its tissue and temporal expression profile were detected...
December 20, 2018: Animal Biotechnology
Malay Shukla, Bheem Dutt Joshi, Ved Prakash Kumar, Mukesh Thakur, Anil Kumar Mehta, Sambandam Sathyakumar, Surendra Prakash Goyal
Musk deer are of high conservation priority owing to poaching pressure because of its musk pod. Representation of musk deer status using genetics is poorly documented in India, and it is not confirmed as to how many species of musk deer are present. We characterize for the first time, the genetic diversity of musk deer from Uttarakhand using Cytochrome Oxidase sub-unit (COI) gene (486 bp) and compared with the data available for other species. Results revealed the presence of six haplotypes in the Uttarakhand population amongst 17 sequences...
December 6, 2018: Animal Biotechnology
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