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Animal Biotechnology

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https://www.readbyqxmd.com/read/28846494/dietary-sodium-butyrate-supplementation-promotes-oxidative-fiber-formation-in-mice
#1
Shuai Chang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jun He, Junqiu Luo, Ping Zheng, Jie Yu, Yuheng Luo
Sodium butyrate (SB), a sodium salt of butyric acid, has been shown to improve the animal production performance. The aim of this work was to test the effect of feeding mice with diets containing different dose of SB (1, 3, and 5%) on oxidative fiber formation. Dietary SB supplementation had no effect on body weights and food intakes. Dietary SB supplementation upregulated the expressions of oxidative fiber-related protein including MyHC I, MyHC IIa, myoglobin, and troponin-I-slow. Dietary SB supplementation also upregulated the expressions of phospho-FoxO1 and MEF2C protein, but did not affect total FoxO1 protein expression...
August 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28846493/lncrnas-in-secondary-hair-follicle-of-cashmere-goat-identification-expression-and-their-regulatory-network-in-wnt-signaling-pathway
#2
Wen L Bai, Su J Zhao, Ze Y Wang, Yu B Zhu, Yun L Dang, Yu Y Cong, Hui L Xue, Wei Wang, Liang Deng, Dan Guo, Shi Q Wang, Yan X Zhu, Rong H Yin
Long noncoding RNAs (lncRNAs) are a novel class of eukaryotic transcripts. They are thought to act as a critical regulator of protein-coding gene expression. Herein, we identified and characterized 13 putative lncRNAs from the expressed sequence tags from secondary hair follicle of Cashmere goat. Furthermore, we investigated their transcriptional pattern in secondary hair follicle of Liaoning Cashmere goat during telogen and anagen phases. Also, we generated intracellular regulatory networks of upregulated lncRNAs at anagen in Wnt signaling pathway based on bioinformatics analysis...
August 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28799860/mutations-in-leptin-lep-gene-are-associated-with-carcass-and-meat-quality-traits-in-crossbreed-rabbits
#3
Lukasz Migdal, Konrad Koziol, Sylwia Palka, Wladyslaw Migdal, Tomasz Zabek, Agnieszka Otwinowska-Mindur, Anna Migdal, Michal Kmiecik, Dorota Maj, Jozef Bieniek
Leptin is a hormone synthesized and secreted primarily in adipose cells that help to regulate energy balance. This study examined the associations of single nucleotide polymorphisms in the rabbit leptin gene with growth traits, slaughter traits and physicochemical parameters of New Zealand White (NZW) and Belgian Giant Grey (BGG) crossbreed rabbits. In total, 320 crossbreed animals were genotyped for polymorphisms within exon 2-g.16081633T>C, intron 1_2-g.16081420C>T, and within UTR-g.16079636C>G for association analysis...
August 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28799830/effects-of-microrna-27a-on-myogenin-expression-and-akt-foxo1-signal-pathway-during-porcine-myoblast-differentiation
#4
Shurun Zhang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Jun He, Ping Zheng, Jie Yu, Junqiu Luo, Yuheng Luo, Hong Chen
Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a kind of small noncoding RNAs that have been regarded as important regulators in skeletal muscle cell proliferation and differentiation. The objective of this study was to investigate the effects of microRNA-27a (miR-27a) on myogenin expression and Akt/FoxO1 signal pathway during porcine myoblast differentiation...
August 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28799828/molecular-characterization-and-comparison-of-phospholipase-c-zeta-plcz1-gene-between-swamp-bubalus-carabanensis-and-riverine-bubalus-bubalis-buffaloes-its-implications-and-future-perspectives
#5
Eufrocina P Atabay, Roseline D Tadeo, Edwin C Atabay, Emma V Venturina, Rafael A Fissore, Claro N Mingala
Phospholipase C zeta, a novel sperm-specific protein which is widely known to induce oocyte activation following fertilization, had already been characterized in various mammalian species, but not in water buffaloes thus far. The present study was conducted to initially characterize and compare the sequences of PLCZ1 gene of swamp and riverine buffaloes. Semen samples were collected; total RNA was extracted and reverse-transcribed. PLCZ1 cDNA was then amplified, and submitted for sequencing. Buffalo PLCZ1 gene yielded a sequence of 1905 base pair nucleotides translated into 634 bp amino acids...
August 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28799826/multiplex-assay-for-identifying-animal-species-found-in-the-tibetan-area-using-the-mitochondrial-12s-rrna-gene
#6
Jung Nam Lee, MingFeng Jiang, YongLi Wen, ShiLin Li, GuoRong Yuan
Southwestern China has an area with unique natural conditions located in alpine regions at altitudes from 2000 to 5000 m; this area is referred to as the Qinghai-Tibetan plateau (QTP). Unique animals, such as yaks (Bos grunniens), are found extensively on the plateau of Southwestern China due to its unique environment. In recent years, the prevalence of fake meat products such as fake jerky has increased in this area. This research was conducted as an attempt to develop a reliable multiplex polymerase chain reaction (mPCR) detection method for identifying nine animal species found in QTP...
August 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28662369/generation-of-genomic-deletions-of-rig-i-gene-in-goat-primary-cell-culture-using-crispr-cas9-method
#7
Shivani Malpotra, Ashutosh Vats, Sushil Kumar, Devika Gautam, Sachinandan De
CRISPR/Cas9 system is a natural immune system in prokaryotes protecting them from infectious viral or plasmid DNA invading the cells. This RNA-guided system can act as powerful tool for introducing genomic alterations in eukaryotic cells with high efficiency. In the present study, Rig-Igene is taken as model gene to study the efficiency of CRISPR/Cas9 system induced gene deletion in primary fibroblast cell culture. Rig-I(retinoic acid-inducible gene-1) is involved in regulating immune response in mammals. In this study, we optimized the CRISPR/Cas9 method for knocking out Rig-Igene in Goat primary fibroblasts by using a NHEJ pathway...
June 29, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28658593/effects-of-soy-lecithin-extender-on-dog-sperm-cryopreservation
#8
Andressa Dalmazzo, João Diego A Losano, Carolina C Rocha, Roberta H Tsunoda, Daniel de Souza Ramos Angrimani, Camilla M Mendes, Mayra Elena O D Ávila Assumpção, Marcilio Nichi, Valquíria H Barnabe
Semen cryopreservation is an essential biotechnology in canine reproduction and during the cryopreservation process commonly egg yolk are used. The discrepancy in the egg yolk composition and the potential risk of disease dissemination are obstacles for semen exportation and use. Therefore, studies aiming to substitute egg yolk are extremely important. In this context, soy lecithin contains a low-density lipoprotein fraction, is an interesting alternative. Thus, the objective of this study was to compare extenders based on soy lecithin (several concentrations and forms) with egg yolk during the cryopreservation process of dog sperm...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28657495/in-silico-structural-studies-and-molecular-docking-analysis-of-delta6-desaturase-in-hufa-biosynthetic-pathway
#9
Suvra Roy, Hirak Jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Fish are an important source of highly unsaturated fatty acids (HUFA) such as eicosapentaenoic acid EPA (20:5 n-3) and docosahexaenoic acid DHA (22:6 n-3) and play a significant role in human nutrition. The fatty acyl delta6-desaturase (Δ6 desaturase) is a rate-limiting enzyme in the biosynthetic pathway of highly unsaturated fatty acids (HUFA) that converts polyunsaturated fatty acids (PUFA) such as linoleic (18:2n-6) and α-linolenic (18:3n-3) acids into HUFA. In this study, fatty acyl Δ6 desaturase was identified from pangasius (Pangasianodon hypophthalmus) and further analyzed for sequenced-based characterization and 3D structural conformation...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28657419/hepatoprotective-hypoglycemic-and-hypolipidemic-effect-of-chokeberry-pomace-on-polish-merino-lambs
#10
Paulina Lipińska, Artur Jóźwik
To properly understand the biological effect of polyphenol compounds, it should not be assumed that they are generally bioavailable and are reaching tissues as expected. It is important to fully understand how polyphenol compounds are absorbed, metabolized, and finally eliminated from the organism. Most polyphenol compounds occur in the forms that cannot be absorbed in their primary form. Sheep make compounds bioavailable due to their unique digestive system properties. Despite this, several species of ruminants likely to serve as an experimental model were considered...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28636491/atherosclerosis-induced-by-a-high-cholesterol-and-high-fat-diet-in-the-inbred-strain-of-the-wuzhishan-miniature-pig
#11
Yuqiong Zhao, Lei Xiang, Yaqian Liu, Miaomiao Niu, Jifang Yuan, Hua Chen
Coronary artery disease has a significant genetic predisposition, which mainly results from atherosclerosis. Miniature pig is an excellent model to investigate atherosclerosis. This study investigated whether the occurrence and development of atherosclerosis in the Wuzhishan miniature pigs (WZSPs) that were closely bred 12 generations had better consistency. The WZSPs (n = 9) were fed a high-cholesterol and high-fat diet (HCFD). After continuous feeding, 3 WZSPs each were sacrificed at 6, 8, and 12 months, respectively, and the general clinical manifestations and serological indexes were detected...
June 21, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28636460/evaluation-of-bovine-high-density-snp-genotyping-array-in-indigenous-dairy-cattle-breeds
#12
S Dash, A Singh, A K Bhatia, S Jayakumar, A Sharma, S Singh, I Ganguly, S P Dixit
In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their performance. Only 40%-50% SNPs of these arrays was found informative for genetic analysis in these cattle breeds. The overall mean of MAF for SNPs of BovineHD SNPChip was 0.248 ± 0.006, 0.241 ± 0...
June 21, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28594279/single-nucleotide-polymorphisms-in-5-upstream-region-of-bovine-tlr4-gene-affecting-expression-profile-and-transcription-factor-binding-sites
#13
Chinmoy Mishra, Subodh Kumar, Manjit Panigrahi, H M Yathish, Rajni Chaudhary, Anuj Chauhan, Amit Kumar, Arvind A Sonawane
The present study in the 5' upstream region of TLR4 gene revealed four Single Nucleotide Polymorphisms (SNPs) in Vrindavani and Tharparkar cattle. The polymorphic information content (PIC), heterozygosity and allelic diversity values were low to moderate for these SNPs. In Vrindavani cattle, one SNP was found to be in Hardy-Weinberg Equilibrium (HWE) and the remaining three were found to be in linkage disequilibrium (LD) as indicated statistically (P > 0.05). In Tharparkar cattle, two SNPs were found to be in HWE and were not in LD as indicated statistically (P > 0...
June 8, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28537816/effect-of-vitamin-e-and-polyunsaturated-fatty-acids-on-cryopreserved-sperm-quality-in-bos-taurus-bulls-under-testicular-heat-stress
#14
João D A Losano, Daniel S R Angrimani, Andressa Dalmazzo, Carolina C Rocha, Maíra M Brito, Eduardo G A Perez, Roberta H Tsunoda, Paola A A Góes, Camilla M Mendes, Mayra E O A Assumpção, Valquiria H Barnabe, Marcilio Nichi
Taurine bulls are highly susceptible to heat stress, leading to increased oxidative stress (OS) and impaired sperm viability. Polyunsaturated fatty acids (PUFAs) supplementation can be an alternative to improve semen quality, which also results in more sperm susceptibility to lipid peroxidation. Moreover, this deleterious effect can be exacerbated in animals affected by heat stress. Vitamin E is a key antioxidant that counteracts lipid peroxidation of sperm membrane caused by OS. Thus, combining PUFAs with vitamin E may improve sperm quality...
May 24, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28494220/effects-of-recombinant-human-lactoferrin-on-osteoblast-growth-and-bone-status-in-piglets
#15
Qiuling Li, Jie Zhao, Wenping Hu, Jianwu Wang, Tian Yu, Yunping Dai, Ning Li
Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regarding the effects of recombinant human LF (rhLF) supplementation on bone status in young healthy infants. We found osteoblast cell differentiation was significantly promoted in vitro. Furthermore, treatment of human osteoblast cells with rhLF rapidly induced phosphorylation of p44/p42 mitogen-activated protein kinase (p44/p42 MAPK, ERK1/2)...
May 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28489974/diversity-of-the-tlr4-immunity-receptor-in-czech-native-cattle-breeds-revealed-using-the-pacific-biosciences-sequencing-platform
#16
Karel Novák, Jitka Pikousová, Vladimíra Czerneková, Věra Mátlová
The allelic variants of immunity genes in historical breeds likely reflect local infection pressure and therefore represent a reservoir for breeding. Screening to determine the diversity of the Toll-like receptor gene TLR4 was conducted in two conserved cattle breeds: Czech Red and Czech Red Pied. High-throughput sequencing of pooled PCR amplicons using the PacBio platform revealed polymorphisms, which were subsequently confirmed via genotyping techniques. Eight SNPs found in coding and adjacent regions were grouped into 18 haplotypes, representing a significant portion of the known diversity in the global breed panel and presumably exceeding diversity in production populations...
May 10, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28489967/associations-of-the-polymorphisms-in-dhrs4-serping1-and-apor-genes-with-postmortem-ph-in-berkshire-pigs
#17
Jung Hye Hwang, Sang Mi An, Seul Gi Kwon, Da Hye Park, Tae Wan Kim, Deok Gyung Kang, Go Eun Yu, Il-Suk Kim, Hwa Chun Park, Jeongim Ha, Chul Wook Kim
Postmortem pH is a main factor influencing the meat quality in pigs. This study investigated the association of postmortem pH with single-nucleotide polymorphisms (SNPs) in the fourth member of the short-chain dehydrogenase/reductase family (DHRS4), the first member of serpin peptidase inhibitor, clade G (complement inhibitor) (SERPING1), and the apolipoprotein R precursor (APOR) genes in Berkshire pigs. The study included 437 pigs, and genotyping was conducted using the GoldenGate Assay (Illumina, San Diego, CA, USA)...
May 10, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28481711/two-novel-snps-of-ppar%C3%AE-significantly-affect-weaning-growth-traits-of-nanyang-cattle
#18
Jieping Huang, Ningbo Chen, Xin Li, Shanshan An, Minghui Zhao, Taihong Sun, Ruijie Hao, Yun Ma
Peroxisome-proliferator-activated receptor gamma (PPARγ) is a key transcription factor that controls adipocyte differentiation and energy in mammals. Therefore, PPARγ is a potential factor influencing animal growth traits. This study primarily evaluates PPARγ as candidate gene for growth traits of cattle and identifies potential molecular marker for cattle breeding. Per previous studies, PPARγ mRNA was mainly expressed at extremely high levels in adipose tissues as shown by quantitative real-time polymerase chain reaction analysis...
May 8, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28471716/polymorphisms-in-mx2-gene-are-related-with-scs-in-chinese-dairy-cows
#19
Ningbo Chen, FengQiao Wang, Nongqi Yu, Yuan Gao, Jieping Huang, Ruihua Dang, Yongzhen Huang, Xianyong Lan, Chuzhao Lei, Hong Chen
Viral infections can play direct or indirect roles in the etiology of the bovine mastitis. Mx dynamin-like GTPase 2 (MX2) gene is a main effector of the antiviral innate immune defense mediated by type I interferon (IFN I), which was demonstrated to confer positive antiviral responses to many viruses. Given the importance of the MX2 in modulating the host immune response, MX2 gene may be a suitable candidate gene for studying disease resistance in dairy cattle. Here, we scanned the sequence variation of the MX2 gene in Chinese indigenous cattle breeds...
May 4, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27854153/genetic-factors-affecting-pork-quality-halothane-and-rendement-napole-genes
#20
REVIEW
Ramon Cesar D Salas, Claro N Mingala
The most common pork quality problems are pale, soft, and exudative (PSE) and acid pork (AP). PSE is associated with the expression of recessive halothane (Hal) allele Hal(n). Recessive Hal pigs (Hal(nn)) have defective Ca(2+) release channels (CRC) or Ryanodine Receptors (RYR1) within the sarcoplasmic reticulum that allow uncontrolled release of Ca(2+) in response to stress. Abnormal lactic acid metabolism caused by stress prior to slaughter leads to the sudden drop in postmortem muscle pH producing the PSE pork...
April 3, 2017: Animal Biotechnology
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