journal
MENU ▼
Read by QxMD icon Read
search

Animal Biotechnology

journal
https://www.readbyqxmd.com/read/28662369/generation-of-genomic-deletions-of-rig-i-gene-in-goat-primary-cell-culture-using-crispr-cas9-method
#1
Shivani Malpotra, Ashutosh Vats, Sushil Kumar, Devika Gautam, Sachinandan De
CRISPR/Cas9 system is a natural immune system in prokaryotes protecting them from infectious viral or plasmid DNA invading the cells. This RNA-guided system can act as powerful tool for introducing genomic alterations in eukaryotic cells with high efficiency. In the present study, Rig-Igene is taken as model gene to study the efficiency of CRISPR/Cas9 system induced gene deletion in primary fibroblast cell culture. Rig-I(retinoic acid-inducible gene-1) is involved in regulating immune response in mammals. In this study, we optimized the CRISPR/Cas9 method for knocking out Rig-Igene in Goat primary fibroblasts by using a NHEJ pathway...
June 29, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28658593/effects-of-soy-lecithin-extender-on-dog-sperm-cryopreservation
#2
Andressa Dalmazzo, João Diego A Losano, Carolina C Rocha, Roberta H Tsunoda, Daniel de Souza Ramos Angrimani, Camilla M Mendes, Mayra Elena O D Ávila Assumpção, Marcilio Nichi, Valquíria H Barnabe
Semen cryopreservation is an essential biotechnology in canine reproduction and during the cryopreservation process commonly egg yolk are used. The discrepancy in the egg yolk composition and the potential risk of disease dissemination are obstacles for semen exportation and use. Therefore, studies aiming to substitute egg yolk are extremely important. In this context, soy lecithin contains a low-density lipoprotein fraction, is an interesting alternative. Thus, the objective of this study was to compare extenders based on soy lecithin (several concentrations and forms) with egg yolk during the cryopreservation process of dog sperm...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28657495/in-silico-structural-studies-and-molecular-docking-analysis-of-delta6-desaturase-in-hufa-biosynthetic-pathway
#3
Suvra Roy, Hirak Jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Fish are an important source of highly unsaturated fatty acids (HUFA) such as eicosapentaenoic acid EPA (20:5 n-3) and docosahexaenoic acid DHA (22:6 n-3) and play a significant role in human nutrition. The fatty acyl delta6-desaturase (Δ6 desaturase) is a rate-limiting enzyme in the biosynthetic pathway of highly unsaturated fatty acids (HUFA) that converts polyunsaturated fatty acids (PUFA) such as linoleic (18:2n-6) and α-linolenic (18:3n-3) acids into HUFA. In this study, fatty acyl Δ6 desaturase was identified from pangasius (Pangasianodon hypophthalmus) and further analyzed for sequenced-based characterization and 3D structural conformation...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28657419/hepatoprotective-hypoglycemic-and-hypolipidemic-effect-of-chokeberry-pomace-on-polish-merino-lambs
#4
Paulina Lipińska, Artur Jóźwik
To properly understand the biological effect of polyphenol compounds, it should not be assumed that they are generally bioavailable and are reaching tissues as expected. It is important to fully understand how polyphenol compounds are absorbed, metabolized, and finally eliminated from the organism. Most polyphenol compounds occur in the forms that cannot be absorbed in their primary form. Sheep make compounds bioavailable due to their unique digestive system properties. Despite this, several species of ruminants likely to serve as an experimental model were considered...
June 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28636491/atherosclerosis-induced-by-a-high-cholesterol-and-high-fat-diet-in-the-inbred-strain-of-the-wuzhishan-miniature-pig
#5
Yuqiong Zhao, Lei Xiang, Yaqian Liu, Miaomiao Niu, Jifang Yuan, Hua Chen
Coronary artery disease has a significant genetic predisposition, which mainly results from atherosclerosis. Miniature pig is an excellent model to investigate atherosclerosis. This study investigated whether the occurrence and development of atherosclerosis in the Wuzhishan miniature pigs (WZSPs) that were closely bred 12 generations had better consistency. The WZSPs (n = 9) were fed a high-cholesterol and high-fat diet (HCFD). After continuous feeding, 3 WZSPs each were sacrificed at 6, 8, and 12 months, respectively, and the general clinical manifestations and serological indexes were detected...
June 21, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28636460/evaluation-of-bovine-high-density-snp-genotyping-array-in-indigenous-dairy-cattle-breeds
#6
S Dash, A Singh, A K Bhatia, S Jayakumar, A Sharma, S Singh, I Ganguly, S P Dixit
In total 52 samples of Sahiwal ( 19 ), Tharparkar ( 17 ), and Gir ( 16 ) were genotyped by using BovineHD SNP chip to analyze minor allele frequency (MAF), genetic diversity, and linkage disequilibrium among these cattle. The common SNPs of BovineHD and 54K SNP Chips were also extracted and evaluated for their performance. Only 40%-50% SNPs of these arrays was found informative for genetic analysis in these cattle breeds. The overall mean of MAF for SNPs of BovineHD SNPChip was 0.248 ± 0.006, 0.241 ± 0...
June 21, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28594279/single-nucleotide-polymorphisms-in-5-upstream-region-of-bovine-tlr4-gene-affecting-expression-profile-and-transcription-factor-binding-sites
#7
Chinmoy Mishra, Subodh Kumar, Manjit Panigrahi, H M Yathish, Rajni Chaudhary, Anuj Chauhan, Amit Kumar, Arvind A Sonawane
The present study in the 5' upstream region of TLR4 gene revealed four Single Nucleotide Polymorphisms (SNPs) in Vrindavani and Tharparkar cattle. The polymorphic information content (PIC), heterozygosity and allelic diversity values were low to moderate for these SNPs. In Vrindavani cattle, one SNP was found to be in Hardy-Weinberg Equilibrium (HWE) and the remaining three were found to be in linkage disequilibrium (LD) as indicated statistically (P > 0.05). In Tharparkar cattle, two SNPs were found to be in HWE and were not in LD as indicated statistically (P > 0...
June 8, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28537816/effect-of-vitamin-e-and-polyunsaturated-fatty-acids-on-cryopreserved-sperm-quality-in-bos-taurus-bulls-under-testicular-heat-stress
#8
João D A Losano, Daniel S R Angrimani, Andressa Dalmazzo, Carolina C Rocha, Maíra M Brito, Eduardo G A Perez, Roberta H Tsunoda, Paola A A Góes, Camilla M Mendes, Mayra E O A Assumpção, Valquiria H Barnabe, Marcilio Nichi
Taurine bulls are highly susceptible to heat stress, leading to increased oxidative stress (OS) and impaired sperm viability. Polyunsaturated fatty acids (PUFAs) supplementation can be an alternative to improve semen quality, which also results in more sperm susceptibility to lipid peroxidation. Moreover, this deleterious effect can be exacerbated in animals affected by heat stress. Vitamin E is a key antioxidant that counteracts lipid peroxidation of sperm membrane caused by OS. Thus, combining PUFAs with vitamin E may improve sperm quality...
May 24, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28494220/effects-of-recombinant-human-lactoferrin-on-osteoblast-growth-and-bone-status-in-piglets
#9
Qiuling Li, Jie Zhao, Wenping Hu, Jianwu Wang, Tian Yu, Yunping Dai, Ning Li
Lactoferrin (LF), an ~80 kDa iron-binding glycoprotein, modulates many biological effects, including antimicrobial and immunomodulatory activities. Recently, it was shown that LF also regulates bone cell activity, suggesting its therapeutic effect on postmenopausal bone loss. However, a minimal amount is known regarding the effects of recombinant human LF (rhLF) supplementation on bone status in young healthy infants. We found osteoblast cell differentiation was significantly promoted in vitro. Furthermore, treatment of human osteoblast cells with rhLF rapidly induced phosphorylation of p44/p42 mitogen-activated protein kinase (p44/p42 MAPK, ERK1/2)...
May 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28489974/diversity-of-the-tlr4-immunity-receptor-in-czech-native-cattle-breeds-revealed-using-the-pacific-biosciences-sequencing-platform
#10
Karel Novák, Jitka Pikousová, Vladimíra Czerneková, Věra Mátlová
The allelic variants of immunity genes in historical breeds likely reflect local infection pressure and therefore represent a reservoir for breeding. Screening to determine the diversity of the Toll-like receptor gene TLR4 was conducted in two conserved cattle breeds: Czech Red and Czech Red Pied. High-throughput sequencing of pooled PCR amplicons using the PacBio platform revealed polymorphisms, which were subsequently confirmed via genotyping techniques. Eight SNPs found in coding and adjacent regions were grouped into 18 haplotypes, representing a significant portion of the known diversity in the global breed panel and presumably exceeding diversity in production populations...
May 10, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28489967/associations-of-the-polymorphisms-in-dhrs4-serping1-and-apor-genes-with-postmortem-ph-in-berkshire-pigs
#11
Jung Hye Hwang, Sang Mi An, Seul Gi Kwon, Da Hye Park, Tae Wan Kim, Deok Gyung Kang, Go Eun Yu, Il-Suk Kim, Hwa Chun Park, Jeongim Ha, Chul Wook Kim
Postmortem pH is a main factor influencing the meat quality in pigs. This study investigated the association of postmortem pH with single-nucleotide polymorphisms (SNPs) in the fourth member of the short-chain dehydrogenase/reductase family (DHRS4), the first member of serpin peptidase inhibitor, clade G (complement inhibitor) (SERPING1), and the apolipoprotein R precursor (APOR) genes in Berkshire pigs. The study included 437 pigs, and genotyping was conducted using the GoldenGate Assay (Illumina, San Diego, CA, USA)...
May 10, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28481711/two-novel-snps-of-ppar%C3%AE-significantly-affect-weaning-growth-traits-of-nanyang-cattle
#12
Jieping Huang, Ningbo Chen, Xin Li, Shanshan An, Minghui Zhao, Taihong Sun, Ruijie Hao, Yun Ma
Peroxisome-proliferator-activated receptor gamma (PPARγ) is a key transcription factor that controls adipocyte differentiation and energy in mammals. Therefore, PPARγ is a potential factor influencing animal growth traits. This study primarily evaluates PPARγ as candidate gene for growth traits of cattle and identifies potential molecular marker for cattle breeding. Per previous studies, PPARγ mRNA was mainly expressed at extremely high levels in adipose tissues as shown by quantitative real-time polymerase chain reaction analysis...
May 8, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28471716/polymorphisms-in-mx2-gene-are-related-with-scs-in-chinese-dairy-cows
#13
Ningbo Chen, FengQiao Wang, Nongqi Yu, Yuan Gao, Jieping Huang, Ruihua Dang, Yongzhen Huang, Xianyong Lan, Chuzhao Lei, Hong Chen
Viral infections can play direct or indirect roles in the etiology of the bovine mastitis. Mx dynamin-like GTPase 2 (MX2) gene is a main effector of the antiviral innate immune defense mediated by type I interferon (IFN I), which was demonstrated to confer positive antiviral responses to many viruses. Given the importance of the MX2 in modulating the host immune response, MX2 gene may be a suitable candidate gene for studying disease resistance in dairy cattle. Here, we scanned the sequence variation of the MX2 gene in Chinese indigenous cattle breeds...
May 4, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27854153/genetic-factors-affecting-pork-quality-halothane-and-rendement-napole-genes
#14
REVIEW
Ramon Cesar D Salas, Claro N Mingala
The most common pork quality problems are pale, soft, and exudative (PSE) and acid pork (AP). PSE is associated with the expression of recessive halothane (Hal) allele Hal(n). Recessive Hal pigs (Hal(nn)) have defective Ca(2+) release channels (CRC) or Ryanodine Receptors (RYR1) within the sarcoplasmic reticulum that allow uncontrolled release of Ca(2+) in response to stress. Abnormal lactic acid metabolism caused by stress prior to slaughter leads to the sudden drop in postmortem muscle pH producing the PSE pork...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27824510/association-of-bf-rbp4-and-esr2-genotypes-with-litter-size-in-an-autochthonous-pig-population
#15
G P Laliotis, A Marantidis, M Avdi
The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observed for the examined loci, while the B allele noted to be the more frequent in all analyzed genes. In addition, sows with the AA genotype of BF gene found to produce significantly lower numbers of the total born piglets (TNB) and number of piglets born alive (TNA), while the respective BB genotype significantly exceeded in TNB and NBA traits compared to the other two genotypes (P < 0...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27813832/single-nucleotide-polymorphisms-in-il8-and-tlr4-genes-as-candidates-for-digital-dermatitis-resistance-susceptibility-in-holstein-cattle
#16
El-Sayed El-Shafaey, Ahmed Ateya, Hazem Ramadan, Rasha Saleh, Yousef Elseady, Eman Abo El Fadl, Sabry El-Khodery
Relatedness between single nucleotide polymorphisms in IL8 and TLR4 genes and digital dermatitis resistance/susceptibility was investigated in seventy Holstein dairy cows. Animals were assigned into two groups, affected group (n = 35) and resistant group (n = 35) based on clinical signs and previous history of farm clinical records. Blood samples were collected for DNA extraction to ampliy fragments of 267-bp and 382-bp for IL8 and TLR4 genes, respectively. PCR-DNA sequencing revealed three SNPs in each of IL8 and TLR4 genes...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27791596/antiviral-compounds-against-nucleocapsid-protein-of-porcine-epidemic-diarrhea-virus
#17
Nipaporn Deejai, Yaowaluck Maprang Roshorm, Anchanee Kubera
Porcine epidemic diarrhea (PED) is a severe diarrhea disease in swine that is caused by porcine epidemic diarrhea virus (PEDV). Nucleocapsid (N) protein is the RNA-binding protein of PEDV, which plays an important role for virus life cycle. The aim of this research was to screen and characterize the compounds that could inhibit the activity of PEDV N protein. The gene encoding PEDV N protein obtained from PEDV Thai isolate was cloned and expressed in E. coli. Its amino acid sequence was employed to generate the three dimensional structure by homology modeling...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27791476/polymorphisms-of-the-toll-like-receptor-2-of-goats-capra-hircus-may-be-associated-with-somatic-cell-count-in-milk
#18
Christina T Ruiz-Rodriguez, Jessica R Brandt, Ryan Oliverio, Yasuko Ishida, Noa Guedj, Edgar F Garrett, Gila Kahila Bar-Gal, Nikolas Nikolaidis, Felipe C Cardoso, Alfred L Roca
Toll-like receptor 2 (TLR2) plays an important role in recognition by the innate immune system of Gram-positive bacteria. As Gram-positive bacteria cause mastitis, we examined variations in the region of the TLR2 gene that codes for the extracellular domain. Samples of forty goats from a single dairy herd were collected, half with low SCC (≤200,000 cells/mL), and half with higher SCC. Two synonymous single nucleotide polymorphisms (SNPs) were identified: 840G > A and 1083A > G. One nonsynonymous SNP 739G > A was identified...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27623989/molecular-cloning-and-expression-profile-of-toll-like-receptor-3-from-an-indian-coldwater-fish-schizothorax-richardsonii-gray
#19
Kiran Belwal, Dimpal Thakuria, Vikas Dighe, Veena Pande, Amit Pande
The innate immune system recognizes pathogens through pattern recognition receptors (PRRs), and toll-like receptors (TLRs) are one of the most important PRRs. TLR3 is a unique member of TLR family that recognizes double-stranded RNA (dsRNA), a viral replication intermediate. There is a variation in its response among diverse fish species toward the same stimulants. We identified and cloned TLR3 from Indian snow trout, Schizothorax richardsonii and carried out its expression analysis in un-induced and poly (I:C) challenged fish...
April 3, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27623936/identification-of-differentially-expressed-genes-and-pathways-for-myofiber-characteristics-in-soleus-muscles-between-chicken-breeds-differing-in-meat-quality
#20
COMPARATIVE STUDY
Y F Du, Q L Ding, Y M Li, W R Fang
In the modern chicken industry, fast-growing broilers have undergone strong artificial selection for muscle growth, which has led to remarkable phenotypic variations compared with slow-growing chickens. However, the molecular mechanism underlying these phenotypes differences remains unknown. In this study, a systematic identification of candidate genes and new pathways related to myofiber development and composition in chicken Soleus muscle (SOL) has been made using gene expression profiles of two distinct breeds: Qingyuan partridge (QY), a slow-growing Chinese breed possessing high meat quality and Cobb 500 (CB), a commercial fast-growing broiler line...
April 3, 2017: Animal Biotechnology
journal
journal
30511
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"