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Animal Biotechnology

Qiuhong Lou, Tingting Li, Pengfei Wu, Cong Qiu, Genxi Zhang, Jinyu Wang
GDF9 (growth differentiation factor 9) belongs to the transforming growth factor-β (TGF-β) superfamily and plays an irreplaceable role in female fertility. To reveal its genetic effects on productivity performance in chickens, 373 Jinghai Yellow chickens were chosen randomly to detect SNPs in GDF9 by PCR-SSCP and DNA sequencing methods. Eventually, four SNPs (g.2053G > A, g.2275T > C, g.2338C > T, g.2420T > C) in total had been detected. Amongst them, g.2420T > C was first found significantly associated with reproduction trait in chickens and heterozygous type C2 T2 had higher average egg weight at 300 days of age (AEWD300) than T2 T2 (p < 0...
November 3, 2018: Animal Biotechnology
Qing Yang, Shaoli Zhang, Jie Li, Xinyu Wang, Kun Peng, Xianyong Lan, Chuanying Pan
The multiplex polymerase chain reaction (PCR) method is fast and accuracy for screening several polymorphism loci in a single reaction in large samples. Therefore, this study aimed to identify three novel insertion/deletion (indel) loci in goat growth hormone receptor (GHR) gene by using multiplex PCR method and the genotypes in three indel loci are identified in 918 individuals from three Chinese goat breeds, as well as to evaluate its associations with growth traits. After validating the accuracy by taking the conventional PCR method, the concordance between these two methods was 100%...
October 31, 2018: Animal Biotechnology
Xiaoqin Wang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jun He, Ping Zheng, Junqiu Luo, Jie Yu, Yuheng Luo
A feeding trial for 91 days was conducted to investigate effects of active immunization against porcine Sox6 (pSox6) on meat quality and myosin heavy chain (MyHC) isoform expression in growing-finishing pigs. Twenty-four castrated Duroc × Landrace × Yarkshire pigs were randomly divided into three groups: (1) Control group; (2) 1 mg/head pSox6 active immunity group; (3) 4 mg/head pSox6 active immunity group (4 mg/head group). The results showed that pigs in 4 mg/head group had a greater a* (Redness) and a higher marbling score, while no significant effect was observed in L* (Lightness), b* (Yellowness), intramuscular fat and cooking loss...
September 28, 2018: Animal Biotechnology
Keng-Chang Tsai, Chen-Wei Chiang, Yan-Ni Lo, Fu-Ling Chang, Tsai-Yu Lin, Chang-Yu Chang, Wang-Chuan Chen, Yu-Ching Lee
The human cluster of differentiation 19 (CD19) is highly expressed in most leukemia, rendering is a promising therapeutic target. In this study, we generated anti-CD19 single-chain variable fragments (scFv) from immunized chickens by phage display technology. After constructing a scFv antibody library with 2.5 × 108 compositional diversity for panning, one representative scFv clone S2 which can specifically recognize to the CD19 protein was isolated and characterized. The binding reactivity of the scFv S2 to the endogenous CD19 protein of the ARH-77 leukemia cancer cell was verified through flow cytometry and the binding affinity of scFv S2 is 6...
September 27, 2018: Animal Biotechnology
Liangliang Xie, Lang Lin, Shihai Huang, Ting Yang, Deshun Shi, Xiangping Li
The low expression of exogenous transferred gene limited the application of transgenic animal technology. Suppressor of variegation 3 ∼ 9 homolog 1(SUV39H1) gene plays a prominent role on repressive heterochromatin and transcription. To understand if exogenous transgenic gene expression was affected by SUV39H1 epigenetic modification, in this paper, the effective shRNA fragments targeting SUV39H1 gene were first screened, their roles on expression of exogenous transgenic genes were determined by using Bcap-37 cell line with stable expressing IFNα-2b gene as a model, the preliminary regulation mechanism of SUV39H1 gene was investigated...
September 4, 2018: Animal Biotechnology
Shah Ahmed Belal, Da Rae Kang, Allur Subramaniyan Sivakumar, Ho Sung Choe, Kwan Seob Shim
This study was conducted to determine the effects of long chain fatty acids (LCFAs) on triacylglycerol (TAG) content, as well as on genes associated with lipid synthesis and fatty acid composition in bovine satellite cells. Both saturated (palmitic and stearic) and unsaturated (oleic and linoleic) fatty acids stimulated the TAG accumulation at a concentration of 100 µM and oleate increased it significantly more than stearate and palmitate. The results revealed that the lipid droplet formation was markedly stimulated by linoleate and oleate at 100 µM...
September 4, 2018: Animal Biotechnology
Panfei Pang, Zhenzhu Li, Hua Hu, Lei Wang, Hua Sun, Shuqi Mei, Fenge Li
To select new Large White line with high number of piglets born, genotypes of estrogen receptor (ESR), the follicle stimulating hormone β subunit (FSHβ), catenin alpha like 1 (CTNNAL1) and miR-27a were tested in 472 Large White sows. The associations of different genotypes with litter size traits were also studied. The results showed ESRBB and FSHβBB sows produced 0.41-1.49 more pigs per litter (p < .05) for total number born (TNB) and number born alive (NBA) than did other corresponding genotypes...
September 4, 2018: Animal Biotechnology
Kajal Kumar Jadav, Ajit Pratap Singh, A B Srivastav, B C Sarkhel
The Indian wild pig is a sub-species (Sus scrofa cristatus) which is different from the other pig breeds and is protected under Schedule-III of the Indian Wildlife (Protection) Act, 1972. In this study, complete mitogenome of two Indian wild pigs was sequenced and characterized by shotgun sequencing and de novo assembly, which revealed sequence size of 16,738 and 16,251 bp, respectively, (Accession no. MG725630 and MG725631). The mitogenome sequence in this study displayed 98% homology with previously reported mitogenome of pigs from different parts of the world...
July 31, 2018: Animal Biotechnology
Tomoko Eguchi-Ogawa, Tatsuya Matsubara, Daisuke Toki, Naohiko Okumura, Asako Ando, Hitoshi Kitagawa, Hirohide Uenishi
Widely used antipig CD4 monoclonal antibodies (mAbs) fail to recognize CD4 alleles characteristic of miniature pig lines such as the National Institutes of Health (NIH) miniature pigs and microminipigs. We surveyed polymorphisms in the coding sequence of the porcine CD4 gene among Western and Oriental pig breeds and Japanese wild boars and investigated their distribution. Of the 13 alleles that we identified among the 47 animals, 2 in group I and 3 in group II were found exclusively in Western breed pigs. Group IV alleles, which included mAb-nonbinding alleles, were found frequently in Oriental breed pigs, suggesting that the mAb-nonbinding allele arose from the gene pool of Oriental pigs...
July 3, 2018: Animal Biotechnology
Yan-Ying Zhao, Ya-Qiu Lin, Ya-Ou Xu
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca2+ binding EF hand and could interact with actin filament...
July 3, 2018: Animal Biotechnology
Muhammad Ibrahim, Sohail Ahmad, Irfan Safdar Durrani, Aqib Iqbal, Iqbal Munir, Zahoor Ahmad Swati
Pakistan is rich in sheep genetic resources. Balkhi, Hashtnagri, and Michni are neighboring sheep populations found in Khyber Pakhtunkhwa province of Pakistan. In this study, we analyzed the genetic structures and bottleneck incidents within these sheep populations using 31 microsatellite DNA markers. Total numbers of 116, 100, and 95 alleles, with average numbers of 3.20, 3.26, and 3.74 alleles per locus were observed, respectively, in Balkhi, Hashtnagri, and Michni population. Mean observed heterozygosity was 0...
July 3, 2018: Animal Biotechnology
Shuai Chang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jun He, Junqiu Luo, Ping Zheng, Jie Yu, Yuheng Luo
Sodium butyrate (SB), a sodium salt of butyric acid, has been shown to improve the animal production performance. The aim of this work was to test the effect of feeding mice with diets containing different dose of SB (1, 3, and 5%) on oxidative fiber formation. Dietary SB supplementation had no effect on body weights and food intakes. Dietary SB supplementation upregulated the expressions of oxidative fiber-related protein including MyHC I, MyHC IIa, myoglobin, and troponin-I-slow. Dietary SB supplementation also upregulated the expressions of phospho-FoxO1 and MEF2C protein, but did not affect total FoxO1 protein expression...
July 3, 2018: Animal Biotechnology
Wen L Bai, Su J Zhao, Ze Y Wang, Yu B Zhu, Yun L Dang, Yu Y Cong, Hui L Xue, Wei Wang, Liang Deng, Dan Guo, Shi Q Wang, Yan X Zhu, Rong H Yin
Long noncoding RNAs (lncRNAs) are a novel class of eukaryotic transcripts. They are thought to act as a critical regulator of protein-coding gene expression. Herein, we identified and characterized 13 putative lncRNAs from the expressed sequence tags from secondary hair follicle of Cashmere goat. Furthermore, we investigated their transcriptional pattern in secondary hair follicle of Liaoning Cashmere goat during telogen and anagen phases. Also, we generated intracellular regulatory networks of upregulated lncRNAs at anagen in Wnt signaling pathway based on bioinformatics analysis...
July 3, 2018: Animal Biotechnology
Shurun Zhang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Jun He, Ping Zheng, Jie Yu, Junqiu Luo, Yuheng Luo, Hong Chen
Skeletal myoblast differentiation is controlled by a multitude of transcription factors and signal pathways. Myogenin is a critical transcriptional regulator in the initiation and maintenance of myoblast differentiation. The Akt/FoxO1 signal pathway plays an important role in myoblast differentiation. MicroRNAs are a kind of small noncoding RNAs that have been regarded as important regulators in skeletal muscle cell proliferation and differentiation. The objective of this study was to investigate the effects of microRNA-27a (miR-27a) on myogenin expression and Akt/FoxO1 signal pathway during porcine myoblast differentiation...
July 3, 2018: Animal Biotechnology
Eufrocina P Atabay, Roseline D Tadeo, Edwin C Atabay, Emma V Venturina, Rafael A Fissore, Claro N Mingala
Phospholipase C zeta, a novel sperm-specific protein which is widely known to induce oocyte activation following fertilization, had already been characterized in various mammalian species, but not in water buffaloes thus far. The present study was conducted to initially characterize and compare the sequences of PLCZ1 gene of swamp and riverine buffaloes. Semen samples were collected; total RNA was extracted and reverse-transcribed. PLCZ1 cDNA was then amplified, and submitted for sequencing. Buffalo PLCZ1 gene yielded a sequence of 1905 base pair nucleotides translated into 634 bp amino acids...
July 3, 2018: Animal Biotechnology
Andressa Dalmazzo, João Diego A Losano, Carolina C Rocha, Roberta H Tsunoda, Daniel de Souza Ramos Angrimani, Camilla M Mendes, Mayra Elena O D Ávila Assumpção, Marcilio Nichi, Valquíria H Barnabe
Semen cryopreservation is an essential biotechnology in canine reproduction and during the cryopreservation process commonly egg yolk are used. The discrepancy in the egg yolk composition and the potential risk of disease dissemination are obstacles for semen exportation and use. Therefore, studies aiming to substitute egg yolk are extremely important. In this context, soy lecithin contains a low-density lipoprotein fraction, is an interesting alternative. Thus, the objective of this study was to compare extenders based on soy lecithin (several concentrations and forms) with egg yolk during the cryopreservation process of dog sperm...
July 3, 2018: Animal Biotechnology
Suvra Roy, Hirak Jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Fish are an important source of highly unsaturated fatty acids (HUFA) such as eicosapentaenoic acid EPA (20:5 n-3) and docosahexaenoic acid DHA (22:6 n-3) and play a significant role in human nutrition. The fatty acyl delta6-desaturase (Δ6 desaturase) is a rate-limiting enzyme in the biosynthetic pathway of highly unsaturated fatty acids (HUFA) that converts polyunsaturated fatty acids (PUFA) such as linoleic (18:2n-6) and α-linolenic (18:3n-3) acids into HUFA. In this study, fatty acyl Δ6 desaturase was identified from pangasius (Pangasianodon hypophthalmus) and further analyzed for sequenced-based characterization and 3D structural conformation...
July 3, 2018: Animal Biotechnology
Kristina Gvozdanović, Vladimir Margeta, Polona Margeta, Ivona Djurkin Kušec, Dalida Galović, Peter Dovč, Goran Kušec
The evaluation of the genetic structure of autochthonous pig breeds is very important for conservation of local pig breeds and preservation of diversity. In this study, 18 microsatellite loci were used to detect genetic relationship between autochthonous pig breeds [Black Slavonian (BS), Turopolje pig (TP), and Croatian wild boar] and to determine phylogenetic relationship among Croatian autochthonous pig breeds and certain Asian and European pigs using the mitochondrial DNA (mtDNA) D-loop sequence polymorphism...
June 23, 2018: Animal Biotechnology
R Kumar Pramod, Dinesh Velayutham, Sajesh P K, Beena P S, Anil Zachariah, Arun Zachariah, Chandramohan B, Sujith S S, Ganapathi P, Bangarusamy Dhinoth Kumar, Sosamma Iype, Ravi Gupta, Sam Santhosh, George Thomas
Indigenous cattle of India belong to the species, Bos indicus and they possess various adaptability and production traits. However, little is known about the genetic diversity and origin of these breeds. To investigate the status, we sequenced and analyzed the whole mitochondrial DNA (mtDNA) of seven Indian cattle breeds. In total, 49 single-nucleotide variants (SNVs) were identified among the seven breeds analyzed. We observed a common synonymous SNV in the COII gene (m.7583G > A) of all the breeds studied...
June 23, 2018: Animal Biotechnology
P B Pantawane, H Dhanze, G V P P S Ravi Kumar, Grace M R, N C Dudhe, K N Bhilegaonkar
Japanese encephalitis (JE) is an emerging mosquito-borne zoonotic flaviviral disease. The present study was undertaken with the objective to develop TaqMan real-time reverse-transcription polymerase chain reaction (RT-PCR) assay for rapid detection and quantification of Japanese encephalitis virus (JEV) in swine blood and mosquito vectors. The amplification of envelope (E) gene was targeted by designing gene-specific MGB TaqMan fluorescent probe along with the primers. The best performance in terms of sensitivity was achieved by standardized TaqMan real-time RT-PCR with a detection limit of 2...
June 23, 2018: Animal Biotechnology
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