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Animal Biotechnology

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https://www.readbyqxmd.com/read/28103144/role-of-different-natural-antioxidants-in-the-modulation-of-mrna-expression-of-apoptotic-molecules-in-the-livers-of-carbon-tetrachloride-intoxicated-rats
#1
Nouf M Al-Rasheed, Naglaa F El-Orabi, Laila Mohamed Fadda, Hanaa Mahmoud Ali, Nawal M Al-Rasheed, Yieldez Bassiouni, Abeer Mohammad Aldbass
Overexpression of nuclear factor (NF-κB) or activation of Smad3 by transforming growth factor β (TGF-β1) induced by oncogenes results in overexpression of fibrotic processes and hence cell death. The objective of this study is to examine whether Silymarin (Sil) alone or in combination with Vitamin E (Vit E) and/or Curcumin (Cur) plays a modulatory role against the overexpression of NF-κB, and TGF-β that induced in response to carbon tetrachloride (CCl4) administration. The present work revealed that CCl4 induced elevation of in serum alanine aminotransferase (ALT), Apoptosis regulator (Bax), Smad3, TGF-β, and NF-kB hepatic mRNA expression (using Real-time PCR), administration of Sil alone downregulated these expressions...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28103142/mapping-of-calf-death-in-japanese-black-cattle
#2
Takashi Hirano, Shota Nishimura, Hiromi Hara, Yoshikazu Sugimoto, Kei Hanzawa
Weak calf syndrome (WCS) is a major cause of calf death in Japanese Black cattle. Among IARS disorders, the isoleucyl-tRNA synthetase c.235G>C mutation has been identified as one of the causes of WCS. However, calf deaths differing from those attributed to IARS disorder has been occurring. To identify other genes potentially responsible for these calf deaths, we constructed three populations of three bulls (Bull-1, -2 and -3) that did not carry the IARS mutation, and dead calves (18, 28, and 31 calves) and healthy cattle (18, 15, and 10 cattle) sired by these bulls...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28103141/modification-of-the-genome-of-domestic-animals
#3
Samantha N Lotti, Kathryn M Polkoff, Marcello Rubessa, Matthew B Wheeler
In the past few years, new technologies have arisen that enable higher efficiency of gene editing. With the increase ease of using gene editing technologies, it is important to consider the best method for transferring new genetic material to livestock animals. Microinjection is a technique that has proven to be effective in mice but is less efficient in large livestock animals. Over the years, a variety of methods have been used for cloning as well as gene transfer including; nuclear transfer, sperm mediated gene transfer (SMGT), and liposome-mediated DNA transfer...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28075701/expression-analysis-of-genes-associated-with-prolificacy-in-fecb-carrier-and-noncarrier-indian-sheep
#4
S Goyal, J Aggarwal, P K Dubey, B P Mishra, P Ghalsasi, C Nimbkar, B K Joshi, R S Kataria
The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development...
January 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28060576/evagreen-based-multiplex-real-time-pcr-assay-for-rapid-differentiation-of-wild-type-and-glycoprotein-e-deleted-bovine-herpesvirus-1-strains
#5
Sachin S Pawar, Chetan D Meshram, Niraj K Singh, Mohini Saini, B P Mishra, Praveen K Gupta
Bovine herpesvirus-1 (BoHV-1) is an important viral pathogen causing significant economic losses to the cattle industry. Glycoprotein E-deleted marker vaccines form the basis for BoHV-1 control programs widely, wherein detection and differentiation of wild-type and gE-deleted vaccine strains is of crucial importance for proper disease management. In the present study, we report an EvaGreen-based multiplex real-time polymerase chain reaction (EGRT-PCR) assay for rapid differentiation of wild-type and glycoprotein E-deleted strains of BoHV-1...
January 6, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28051918/genetic-variation-in-fabp4-and-evaluation-of-its-effects-on-beef-cattle-fat-content
#6
Daniel E Goszczynski, Juliana Papaleo-Mazzucco, María V Ripoli, Edgardo L Villarreal, Andrés Rogberg-Muñoz, Carlos A Mezzadra, Lilia M Melucci, Guillermo Giovambattista
FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms...
January 4, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27874312/momilactone-b-inhibits-ketosis-in-vitro-by-regulating-the-angptl3-lpl-pathway-and-inhibiting-hmgcs2
#7
Dong Young Kang, Nipin S P, Pramod Darvin, Youn Hee Joung, Hyo Joo Byun, Chang Hee Do, Kyung Do Park, Mi Na Park, Kwang Hyun Cho, Young Mok Yang
Ketogenesis is the production of ketone bodies, which provide energy when the body lacks glucose. Under ketogenic conditions, the body switches from primarily carbohydrate to fat metabolism to maintain energy balance. However, accumulation of high levels of ketone bodies in the blood results in ketosis. Treating ketosis with natural substances is preferable, because they are unlikely to cause side-effects. Momilactone B is an active compound isolated from Korean rice. Based on previous studies, we hypothesized that momilactone B could inhibit ketosis...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27874309/abundance-and-characterization-of-perfect-microsatellites-on-the-cattle-y-chromosome
#8
Zhi-Jie Ma
Microsatellites or simple sequence repeats (SSRs) are found in most organisms and play an important role in genomic organization and function. To characterize the abundance of SSRs (1-6 base-pairs [bp]) on the cattle Y chromsome, the relative frequency and density of perfect or uninterrupted SSRs based on the published Y chromosome sequence were examined. A total of 17,273 perfect SSRs were found, with total length of 324.78 kb, indicating that approximately 0.75% of the cattle Y chromosome sequence (43.30 Mb) comprises perfect SSRs, with an average length of 18...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854178/porcine-cloned-embryos-reconstructed-with-the-cell-nuclei-of-tetraploid-m-phase-fibroblast-cells-can-restore-normal-diploidy-at-the-blastocyst-stage
#9
Q Zhao, Y G Qiu, J T Tian, C S Wang, T Z An
The cell cycle of donor cells as a major factor that affects cloning efficiency remains debatable. G2/M phase cells as a donor can successfully produce cloned animals, but a minimal amount is known regarding nuclear remodeling events. In this study, porcine fetal fibroblasts (PFFs) were carefully synchronized at G1 or M phase as donor cells. Most of the cloned embryos reconstructed from PFFs at G1 (G1-embryos) or M (M-embryos) phase formed a pronucleus-like nucleus (PN) within 6-h post fusion (hpf), but the M-embryos formed PN earlier than the G1-embryos did...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854162/endometritis-increases-pro-inflammatory-cytokines-in-follicular-fluid-and-cervico-vaginal-mucus-in-the-buffalo-cow
#10
Jones Boby, Harendra Kumar, Harihar Prasad Gupta, Mustapha Hussain Jan, Sanjay Kumar Singh, Manas Kumar Patra, Sukdeb Nandi, Asha Abraham, Narayanan Krishnaswamy
Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample collection. The present study describes the results of two different experiments. The first experiment was conducted to investigate the influence of endometritis on the proinflammatory cytokines of follicular fluid based on the reproductive tracts of buffalo collected at a slaughter house Buffalo genitalia were categorized into purulent endometritis (PE), cytological endometritis (CE), and non-endometritis (NE) based on the white-side test and endometrial cytology, respectively (n = 14/group)...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854153/genetic-factors-affecting-pork-quality-halothane-and-rendement-napole-genes
#11
Ramon Cesar D Salas, Claro N Mingala
The most common pork quality problems are pale, soft, and exudative (PSE) and acid pork (AP). PSE is associated with the expression of recessive halothane (Hal) allele Hal(n). Recessive Hal pigs (Hal(nn)) have defective Ca(2+) release channels (CRC) or Ryanodine Receptors (RYR1) within the sarcoplasmic reticulum that allow uncontrolled release of Ca(2+) in response to stress. Abnormal lactic acid metabolism caused by stress prior to slaughter leads to the sudden drop in postmortem muscle pH producing the PSE pork...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854152/identification-of-four-snps-in-lhb-gene-and-their-associations-with-sperm-qualities-of-chinese-buffaloes
#12
Yunyun Cheng, Jiaming Gu, Han Xue, Qiang Li, Mingming Liang, Nan Wang, Gang Wang, Qingyan Wu, Songcai Liu, Hao Yu, Jiabao Zhang, Linlin Hao
Luteinizing hormone beta polypeptide (LHB) gene has been considered important for sexual behavior and has associations with sperm quality. In this study, four SNPs (g.276 T>C, g.377A>C, g.401T>C, and g.412A>G) were detected in the LHB gene of 165 water buffaloes by direct sequencing and identification of overlap peaks, each of which was associated with at least one sperm quality trait of ejaculate volume, sperm concentration, post-thaw sperm motilities, and sperm abnormalities by chi-square analysis...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27834588/generation-of-ggta1-mutant-pigs-by-direct-pronuclear-microinjection-of-crispr-cas9-plasmid-vectors
#13
Chin-Kai Chuang, Chien-Hong Chen, Chung-Ling Huang, Yu-Hsiu Su, Shu-Hui Peng, Tai-Yun Lin, Hao-Chih Tai, Tien-Shuh Yang, Ching-Fu Tu
This study was conducted to confirm that 1-site and 4-site ppU6-GGTA1-gRNA CRISPR vectors together with the pCX-Flag2-NLS1-Cas9-NLS2 plasmid can both generate KO pigs by direct pronuclear microinjection. In total, 41 and 53 fertilized eggs were microinjected on 1-site and 4-site strategies, respectively. The 1-site construction generated a litter of 8 piglets, and 2 were mono-allelic mutant (mMt). The injection of 4-site constructions resulted in one biallelic mutant (bMt) and one mMt piglet in a litter of 7...
November 11, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27824510/association-of-bf-rbp4-and-esr2-genotypes-with-litter-size-in-an-autochthonous-pig-population
#14
G P Laliotis, A Marantidis, M Avdi
: The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observed for the examined loci, while the B allele noted to be the more frequent in all analyzed genes. In addition, sows with the AA genotype of BF gene found to produce significantly lower numbers of the total born piglets (TNB) and number of piglets born alive (TNA), while the respective BB genotype significantly exceeded in TNB and NBA traits compared to the other two genotypes (P < 0...
November 8, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27813832/single-nucleotide-polymorphisms-in-il8-and-tlr4-genes-as-candidates-for-digital-dermatitis-resistance-susceptibility-in-holstein-cattle
#15
El-Sayed El-Shafaey, Ahmed Ateya, Hazem Ramadan, Rasha Saleh, Yousef Elseady, Eman Abo El Fadl, Sabry El-Khodery
Relatedness between single nucleotide polymorphisms in IL8 and TLR4 genes and digital dermatitis resistance/susceptibility was investigated in seventy Holstein dairy cows. Animals were assigned into two groups, affected group (n = 35) and resistant group (n = 35) based on clinical signs and previous history of farm clinical records. Blood samples were collected for DNA extraction to ampliy fragments of 267-bp and 382-bp for IL8 and TLR4 genes, respectively. PCR-DNA sequencing revealed three SNPs in each of IL8 and TLR4 genes...
November 4, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27791596/antiviral-compounds-against-nucleocapsid-protein-of-porcine-epidemic-diarrhea-virus
#16
Nipaporn Deejai, Yaowaluck Maprang Roshorm, Anchanee Kubera
Porcine epidemic diarrhea (PED) is a severe diarrhea disease in swine that is caused by porcine epidemic diarrhea virus (PEDV). Nucleocapsid (N) protein is the RNA-binding protein of PEDV, which plays an important role for virus life cycle. The aim of this research was to screen and characterize the compounds that could inhibit the activity of PEDV N protein. The gene encoding PEDV N protein obtained from PEDV Thai isolate was cloned and expressed in E. coli. Its amino acid sequence was employed to generate the three dimensional structure by homology modeling...
October 28, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27791476/polymorphisms-of-the-toll-like-receptor-2-of-goats-capra-hircus-may-be-associated-with-somatic-cell-count-in-milk
#17
Christina T Ruiz-Rodriguez, Jessica R Brandt, Ryan Oliverio, Yasuko Ishida, Noa Guedj, Edgar F Garrett, Gila Kahila Bar-Gal, Nikolas Nikolaidis, Felipe C Cardoso, Alfred L Roca
Toll-like receptor 2 (TLR2) plays an important role in recognition by the innate immune system of Gram-positive bacteria. As Gram-positive bacteria cause mastitis, we examined variations in the region of the TLR2 gene that codes for the extracellular domain. Samples of forty goats from a single dairy herd were collected, half with low SCC (≤200,000 cells/mL), and half with higher SCC. Two synonymous single nucleotide polymorphisms (SNPs) were identified: 840G > A and 1083A > G. One nonsynonymous SNP 739G > A was identified...
October 28, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27736301/polymorphisms-of-flanking-region-of-the-asb15-gene-and-their-associations-with-performance-traits-in-chicken
#18
Y C Wang, R L Han, Z J Li, J Geng, Y D Tian, R R Jiang, J P Wu, X T Kang
Research on the identity of genes and their relationship with traits of economic importance in chickens could assist in the selection of poultry. In this study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to detect single-nucleotide polymorphisms (SNPs) in the flanking region of the ASB15 gene by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). One SNP of -1271 C>T in 5' flanking region of the chicken ASB15 gene and two SNPs of the 10618 A>G and 10716 G>A in 3' flanking region were identified...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715465/comparison-of-cryopreservation-protocols-single-and-two-steps-and-thawing-fast-and-slow-for-canine-sperm
#19
Maíra M Brito, Cristina F Lúcio, Daniel S R Angrimani, João Diego A Losano, Andressa Dalmazzo, Marcílio Nichi, Camila I Vannucchi
In addition to the existence of several cryopreservation protocols, no systematic research has been carried out in order to confirm the suitable protocol for canine sperm. This study aims to assess the effect of adding 5% glycerol during cryopreservation at 37°C (one-step) and 5°C (two-steps), in addition of testing two thawing protocols (37°C for 30 seconds, and 70°C for 8 seconds). We used 12 sperm samples divided into four experimental groups: Single-Step - Slow Thawing Group; Two-Step - Slow Thawing Group; Single-Step - Fast Thawing Group; and Two-Step - Fast Thawing Group...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715461/correlation-of-the-a-fabp-gene-polymorphism-and-mrna-expression-with-intramuscular-fat-content-in-three-yellow-chicken-and-hetian-black-chicken
#20
Yong Wang, Hongwei Chen, Diangang Han, Ying Chen, Gemingguli Muhatai, Tursunjan Kurban, Jinming Xing, Jianzhong He
The adipocyte-type fatty acid-binding protein (A-FABP) is considered a candidate gene for fat metabolism; thus, it affects fat deposition in chickens. The present study was designed to examine the polymorphism and mRNA abundance of the A-FABP gene with intramuscular fat (IMF) in the pectoralis muscles (PM) and leg muscles (LM) of Three-yellow Chicken (TYC) and Hetian-black Chicken (HTBC). In total, 60 TYCs and 60 HTBCs were sacrificed using exsanguination at market age. The IMF contents of the PM and LM in the HTBC were significantly higher than those in the TYC...
January 2, 2017: Animal Biotechnology
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