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Animal Biotechnology

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https://www.readbyqxmd.com/read/29144199/effect-of-high-hydrostatic-pressure-applied-before-cryopreservation-on-the-survival-rate-and-quality-of-porcine-mesenchymal-stem-cells-after-thawing
#1
Joanna Romanek, Jolanta Opiela, Daniel Lipiński, Zdzisław Smorąg
The aim of the present study was to examine the effects of varied high hydrostatic pressure (HHP) values on survival rate, proliferation rate, cell multipotency (transcript expression of SOX2, C-MYC, and REX1) and apoptosis (expression of phosphatidylserine (PS), SURVIVIN at the RNA level and BAX at the protein level) of porcine mesenchymal stem cells (MSCs). MSCs were isolated from porcine bone marrow and cultured in vitro. Before cryopreservation and storage in liquid nitrogen, MSCs were subjected to HHP at the varied pressures of 20, 30, 40, 50, or 60 MPa for 1 h at 24°C...
November 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29144179/effects-of-active-immunization-against-akirin2-on-muscle-fiber-type-composition-in-pigs
#2
Xiaoling Chen, Yafei Guo, Gang Jia, Hua Zhao, Guangmang Liu, Zhiqing Huang
The objective of this study was to investigate effects of active immunization against Akirin2 on muscle fiber-type composition in pigs. Here we showed that the titer of Akirin2 antibody in pigs immunized with porcine Akirin2 (pAkirin2) was significantly increased. Active immunization against pAkirin2 decreased succinic dehydrogenase and malate dehydrogenase activities and increased lactate dehydrogenase activity in the longissimus dorsi muscle of pigs. Active immunization against pAkirin2 significantly decreased MyHC I and MyHC IIa mRNA expressions and MyHC I protein expression and increased mRNA expressions of MyHC IIb as well as protein expressions of MyHC IIb and fast-MyHC...
November 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29120694/analysis-of-pig-vomeronasal-receptor-type-1-v1r-promoter-region-reveals-a-common-promoter-motif-but-poor-cpg-islands
#3
Hunduma Dinka, Minh Thong Le
Promoters are, generally, located immediately upstream of a transcription start site (TSS) and have a variety of regulatory motifs, such as transcription factors (TFs) and CpG islands (CGIs), that participate in the regulation of gene expression. Here analysis of the promoter region for pig vomeronasal receptor type 1 (V1R) was described. In the analysis, TSSs for pig V1R genes was first identified and five motifs (MV1, MV2, MV3, MV4, and MV5) were found that are shared by at least 50% of the pig V1R promoter input sequences from both strands...
November 9, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29095095/novel-muscle-specific-genes-tcap-tnni1-and-fhl1-in-cattle-snvs-linkage-disequilibrium-combined-genotypes-association-analysis-of-growth-performance-and-carcass-quality-traits-and-expression-studies
#4
Hua He, Zhi-Gang Hu, Sodnompil Tserennadmid, Si Chen, Xiao-Lin Liu
TCAP, TNNI1, and FHL1 regulate muscle growth and development. In this study, four single nucleotide variants (SNVs) were discovered in almost all of the exon and intron regions of the TCAP, TNNI1, and FHL1 genes using DNA pooled sequencing, polymerase chain reaction (PCR)-RFLP, and forced-PCR-RFLP methods in 576 cattle. Four SNVs were significantly associated with the growth performance and carcass quality traits of the cattle. In addition, the haplotype, haplotype frequency, and linkage disequilibrium coefficient of three sequence variants were also evaluated in the cattle population...
November 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035142/distribution-of-the-cd4-alleles-in-sus-scrofa-demonstrates-the-genetic-profiles-of-western-breeds-and-miniature-pigs
#5
Tomoko Eguchi-Ogawa, Tatsuya Matsubara, Daisuke Toki, Naohiko Okumura, Asako Ando, Hitoshi Kitagawa, Hirohide Uenishi
Widely used antipig CD4 monoclonal antibodies (mAbs) fail to recognize CD4 alleles characteristic of miniature pig lines such as the National Institutes of Health (NIH) miniature pigs and microminipigs. We surveyed polymorphisms in the coding sequence of the porcine CD4 gene among Western and Oriental pig breeds and Japanese wild boars and investigated their distribution. Of the 13 alleles that we identified among the 47 animals, 2 in group I and 3 in group II were found exclusively in Western breed pigs. Group IV alleles, which included mAb-nonbinding alleles, were found frequently in Oriental breed pigs, suggesting that the mAb-nonbinding allele arose from the gene pool of Oriental pigs...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035140/cytoskeleton-genes-expression-and-survival-rate-comparison-between-immature-and-mature-yak-oocyte-after-ops-vitrification
#6
Xia Wei, Ye Sijie, Zeng Weibin, Xu Qing, Zheng Jie, Zi Xiangdong
This study was conducted to investigate the effect of vitrification on survival rate and cytoskeleton gene expression during yak oocyte maturation. The yak oocytes were incubated for 0 h [germinal vesicle (GV) stage] and in vitro matured for 24 h [metaphase II (MII) stage] to obtain immature and mature oocytes. Survival rate after vitrification were compared between immature and mature yak oocytes and cytoskeleton-related genes [cytokeratin 8 (CK8), β-actin (ACTB), and gap junction protein, alpha 1 (GJA1)] were tested by real-time PCR...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035139/novel-17-bp-deletion-in-kdm1b-gene-is-significantly-associated-with-testis-weight-in-male-piglet
#7
Yang Cui, Tao Hu, Rui Chen, Shuai Yu, Wuzi Dong, Xiaoyan Lv, Chuanying Pan
Lysine-specific demethylase 1B (KDM1B) which plays a crucial role in regulating methylation status at lysine 4 of histone 3 is important for male fertility. The aim of this study was to explore the KDM1B mRNA expression profiles and to identify novel genetic variants of the pig KDM1B gene, as well as to determine the association between these variants and testis measurement traits in male piglets. The KDM1B mRNA expression profiles indicated that this gene widely expressed in all tested organs. In addition, a novel 17-bp deletion (NC_010449...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035136/functional-identification-of-allograft-inflammatory-factor-1-like-gene-in-luning-chicken
#8
Yan-Ying Zhao, Ya-Qiu Lin, Ya-Ou Xu
Allograft inflammatory factor-1 (AIF-1) is an inflammation-related protein mainly produced by immune cells, such as monocyte/macrophages and activated T lymphocytes. It is essential for the survival and proinflammatory activity of immune cells. However, the function of AIF-1 in chicken still has not been defined. In the present study, AIF-1-like (AIF1L) gene was identified in Luning chicken. Bioinformatics analysis revealed that the molecular weight of the chicken AIF-1 protein was 16290.8 Da. AIF1L contained a Ca(2+) binding EF hand and could interact with actin filament...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035135/draft-de-novo-genome-sequence-of-agapornis-roseicollis-for-application-in-avian-breeding
#9
Henriëtte van der Zwan, Francois van der Westhuizen, Carina Visser, Rencia van der Sluis
In aviculture, lovebirds are considered one of the most popular birds to keep. This African parakeet is known for its range of plumage colors and ease to tame. Plumage variation is the most important price-determining trait of these birds, and also the main selection criterion for breeders. Currently, no genetic screening tests for traits of economic importance or to confirm pedigree data are available for any of the nine lovebird species. As a starting point to develop these tests, the de novo genome of Agapornis roseicollis (rosy-faced lovebird) was sequenced, assembled, and annotated...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29035133/genetic-polymorphism-and-bottleneck-analysis-of-balkhi-hashtnagri-and-michni-sheep-populations-using-microsatellite-markers
#10
Muhammad Ibrahim, Sohail Ahmad, Irfan Safdar Durrani, Aqib Iqbal, Iqbal Munir, Zahoor Ahmad Swati
Pakistan is rich in sheep genetic resources. Balkhi, Hashtnagri, and Michni are neighboring sheep populations found in Khyber Pakhtunkhwa province of Pakistan. In this study, we analyzed the genetic structures and bottleneck incidents within these sheep populations using 31 microsatellite DNA markers. Total numbers of 116, 100, and 95 alleles, with average numbers of 3.20, 3.26, and 3.74 alleles per locus were observed, respectively, in Balkhi, Hashtnagri, and Michni population. Mean observed heterozygosity was 0...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28846494/dietary-sodium-butyrate-supplementation-promotes-oxidative-fiber-formation-in-mice
#11
Shuai Chang, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jun He, Junqiu Luo, Ping Zheng, Jie Yu, Yuheng Luo
Sodium butyrate (SB), a sodium salt of butyric acid, has been shown to improve the animal production performance. The aim of this work was to test the effect of feeding mice with diets containing different dose of SB (1, 3, and 5%) on oxidative fiber formation. Dietary SB supplementation had no effect on body weights and food intakes. Dietary SB supplementation upregulated the expressions of oxidative fiber-related protein including MyHC I, MyHC IIa, myoglobin, and troponin-I-slow. Dietary SB supplementation also upregulated the expressions of phospho-FoxO1 and MEF2C protein, but did not affect total FoxO1 protein expression...
August 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28846493/lncrnas-in-secondary-hair-follicle-of-cashmere-goat-identification-expression-and-their-regulatory-network-in-wnt-signaling-pathway
#12
Wen L Bai, Su J Zhao, Ze Y Wang, Yu B Zhu, Yun L Dang, Yu Y Cong, Hui L Xue, Wei Wang, Liang Deng, Dan Guo, Shi Q Wang, Yan X Zhu, Rong H Yin
Long noncoding RNAs (lncRNAs) are a novel class of eukaryotic transcripts. They are thought to act as a critical regulator of protein-coding gene expression. Herein, we identified and characterized 13 putative lncRNAs from the expressed sequence tags from secondary hair follicle of Cashmere goat. Furthermore, we investigated their transcriptional pattern in secondary hair follicle of Liaoning Cashmere goat during telogen and anagen phases. Also, we generated intracellular regulatory networks of upregulated lncRNAs at anagen in Wnt signaling pathway based on bioinformatics analysis...
August 28, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28631997/expression-and-purification-of-a-functional-porcine-soluble-triggering-receptor-expressed-on-myeloid-cells-1
#13
Hui Li, Shuangshuang Guo, Leyan Yan, Chunhua Meng, Yiyi Hu, Kongwang He, Zhendan Shi
Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1β, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-α) caused by LPS stimulation in the cultured porcine alveolar macrophages...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28489967/associations-of-the-polymorphisms-in-dhrs4-serping1-and-apor-genes-with-postmortem-ph-in-berkshire-pigs
#14
Jung Hye Hwang, Sang Mi An, Seul Gi Kwon, Da Hye Park, Tae Wan Kim, Deok Gyung Kang, Go Eun Yu, Il-Suk Kim, Hwa Chun Park, Jeongim Ha, Chul Wook Kim
Postmortem pH is a main factor influencing the meat quality in pigs. This study investigated the association of postmortem pH with single-nucleotide polymorphisms (SNPs) in the fourth member of the short-chain dehydrogenase/reductase family (DHRS4), the first member of serpin peptidase inhibitor, clade G (complement inhibitor) (SERPING1), and the apolipoprotein R precursor (APOR) genes in Berkshire pigs. The study included 437 pigs, and genotyping was conducted using the GoldenGate Assay (Illumina, San Diego, CA, USA)...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28358284/molecular-cloning-of-porcine-sun5-gene-and-association-between-a-snp-with-litter-size-trait
#15
Zhenhua Zhao, Yonggang Liu
SUN domain-containing protein 5 (SUN5) is an important reproduction related gene. In this study, we cloned the full-length coding sequence of porcine SUN5 gene through RT-PCR. Sequence analysis of this gene revealed that the pig SUN5 gene encodes a protein of 383 amino acids that has high homology with the SUN5 protein of eight species: wild Bactrian camel (95%), alpaca (95%), Yangtze River dolphin (94%), sperm whale (94%), sheep (93%), black flying fox (93%), goat (92%), and horse (91%). This gene is structured into 13 exons and 12 introns as revealed by computer-assisted analysis...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28267421/use-of-pig-as-a-model-for-mesenchymal-stem-cell-therapies-for-bone-regeneration
#16
Marcello Rubessa, Kathryn Polkoff, Massimo Bionaz, Elisa Monaco, Derek J Milner, Scott J Holllister, Michael S Goldwasser, Matthew B Wheeler
Bone is a plastic tissue with a large healing capability. However, extensive bone loss due to disease or trauma requires extreme therapy such as bone grafting or tissue-engineering applications. Presently, bone grafting is the gold standard for bone repair, but presents serious limitations including donor site morbidity, rejection, and limited tissue regeneration. The use of stem cells appears to be a means to overcome such limitations. Bone marrow mesenchymal stem cells (BMSC) have been the choice thus far for stem cell therapy for bone regeneration...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28267420/fto-promotes-adipogenesis-through-inhibition-of-the-wnt-%C3%AE-catenin-signaling-pathway-in-porcine-intramuscular-preadipocytes
#17
Xiaoling Chen, Yanliu Luo, Gang Jia, Guangmang Liu, Hua Zhao, Zhiqing Huang
Numerous studies have demonstrated that FTO plays an important role in adipogenesis. Herein, we designed a small interfering RNA targeting FTO to knock down its endogenous expression and investigated its effects on the proliferation and differentiation of porcine intramuscular preadipocytes. Its possible mechanism was also investigated. We showed that FTO silencing significantly decreased the level of phospho-Histone H3 protein and inhibited the proliferation of porcine intramuscular preadipocytes. In addition, the expressions of peroxisome proliferators-activated receptor γ (PPARγ) and CAAT/enhancer binding protein (C/EBPα) were down-regulated, but the expression of β-catenin was up-regulated, by FTO silencing...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28267411/ovar-mhc-polymorphism-in-malpura-and-avikalin-sheep-vaccinated-for-peste-des-petits-ruminants-ppr-virus
#18
G R Gowane, Najif Akram, L L L Prince, Ved Prakash, Arun Kumar
India harbors a vast diversity of sheep (40 breeds). The study was carried out to assess the genetic diversity of DRB1 and DQA2 locus of the ovar-MHC and their possible association with Peste des petits ruminants (PPR) virus vaccine response in Malpura and Avikalin sheep breeds maintained at an organized institute flock in the semi-arid region of India. Genetic analysis revealed the rich diversity of DRB1 locus with 23 alleles in Malpura and 21 alleles in Avikalin sheep that included 9 new alleles. DQA2 locus also had rich diversity with 19 alleles in Malpura and 20 alleles in Avikalin sheep that included 7 new alleles...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28267410/combined-haplotypes-of-casr-gene-sequence-variants-and-their-associations-with-growth-traits-in-cattle
#19
Binglin Yue, Fuhai Han, Jiyao Wu, Yanhuan Wang, Chunlei Zhang, Xingtang Fang, Xinglei Qi, Yueyu Bai, Hong Chen
The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in cattle breeds. Four novel single nucleotide polymorphisms (SNPs) and one previously reported SNP (NC_007299.5: g.67630865T>C, 67638409G>C, 67660395G>C, 67661546C>G, and 67661892A>C) were identified in the bovine CaSR gene using DNA sequencing and PCR-SSCP methods in 520 individuals from three representative breeds...
October 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28267404/an-investigation-on-the-expression-level-of-interleukin-10-il-10-in-the-healthy-and-mastitic-holstein-cows-and-the-bioinformatics-analysis-of-nucleosome-profile
#20
Bahareh Varzandian, Mostafa Ghaderi-Zefrehei, Saeid Hosseinzadeh, Mostafa Sayyadi, Vahideh Taghadosi, Sara Varzandian
Cytokines are immune regulators that play an essential role in regulating immune response against various infections. The present study focused on the possible association between the expression level of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows in Fars province, Iran, using a quantitative real-time PCR assay. The experimental groups were categorized according to the number of calvings. The expression level of IL-10 was significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones...
October 2, 2017: Animal Biotechnology
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