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Animal Biotechnology

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https://www.readbyqxmd.com/read/28103144/role-of-different-natural-antioxidants-in-the-modulation-of-mrna-expression-of-apoptotic-molecules-in-the-livers-of-carbon-tetrachloride-intoxicated-rats
#1
Nouf M Al-Rasheed, Naglaa F El-Orabi, Laila Mohamed Fadda, Hanaa Mahmoud Ali, Nawal M Al-Rasheed, Yieldez Bassiouni, Abeer Mohammad Aldbass
Overexpression of nuclear factor (NF-κB) or activation of Smad3 by transforming growth factor β (TGF-β1) induced by oncogenes results in overexpression of fibrotic processes and hence cell death. The objective of this study is to examine whether Silymarin (Sil) alone or in combination with Vitamin E (Vit E) and/or Curcumin (Cur) plays a modulatory role against the overexpression of NF-κB, and TGF-β that induced in response to carbon tetrachloride (CCl4) administration. The present work revealed that CCl4 induced elevation of in serum alanine aminotransferase (ALT), Apoptosis regulator (Bax), Smad3, TGF-β, and NF-kB hepatic mRNA expression (using Real-time PCR), administration of Sil alone downregulated these expressions...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28103142/mapping-of-calf-death-in-japanese-black-cattle
#2
Takashi Hirano, Shota Nishimura, Hiromi Hara, Yoshikazu Sugimoto, Kei Hanzawa
Weak calf syndrome (WCS) is a major cause of calf death in Japanese Black cattle. Among IARS disorders, the isoleucyl-tRNA synthetase c.235G>C mutation has been identified as one of the causes of WCS. However, calf deaths differing from those attributed to IARS disorder has been occurring. To identify other genes potentially responsible for these calf deaths, we constructed three populations of three bulls (Bull-1, -2 and -3) that did not carry the IARS mutation, and dead calves (18, 28, and 31 calves) and healthy cattle (18, 15, and 10 cattle) sired by these bulls...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28103141/modification-of-the-genome-of-domestic-animals
#3
Samantha N Lotti, Kathryn M Polkoff, Marcello Rubessa, Matthew B Wheeler
In the past few years, new technologies have arisen that enable higher efficiency of gene editing. With the increase ease of using gene editing technologies, it is important to consider the best method for transferring new genetic material to livestock animals. Microinjection is a technique that has proven to be effective in mice but is less efficient in large livestock animals. Over the years, a variety of methods have been used for cloning as well as gene transfer including; nuclear transfer, sperm mediated gene transfer (SMGT), and liposome-mediated DNA transfer...
January 19, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28075701/expression-analysis-of-genes-associated-with-prolificacy-in-fecb-carrier-and-noncarrier-indian-sheep
#4
S Goyal, J Aggarwal, P K Dubey, B P Mishra, P Ghalsasi, C Nimbkar, B K Joshi, R S Kataria
The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development...
January 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28060576/evagreen-based-multiplex-real-time-pcr-assay-for-rapid-differentiation-of-wild-type-and-glycoprotein-e-deleted-bovine-herpesvirus-1-strains
#5
Sachin S Pawar, Chetan D Meshram, Niraj K Singh, Mohini Saini, B P Mishra, Praveen K Gupta
Bovine herpesvirus-1 (BoHV-1) is an important viral pathogen causing significant economic losses to the cattle industry. Glycoprotein E-deleted marker vaccines form the basis for BoHV-1 control programs widely, wherein detection and differentiation of wild-type and gE-deleted vaccine strains is of crucial importance for proper disease management. In the present study, we report an EvaGreen-based multiplex real-time polymerase chain reaction (EGRT-PCR) assay for rapid differentiation of wild-type and glycoprotein E-deleted strains of BoHV-1...
January 6, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28051918/genetic-variation-in-fabp4-and-evaluation-of-its-effects-on-beef-cattle-fat-content
#6
Daniel E Goszczynski, Juliana Papaleo-Mazzucco, María V Ripoli, Edgardo L Villarreal, Andrés Rogberg-Muñoz, Carlos A Mezzadra, Lilia M Melucci, Guillermo Giovambattista
FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms...
January 4, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27736301/polymorphisms-of-flanking-region-of-the-asb15-gene-and-their-associations-with-performance-traits-in-chicken
#7
Y C Wang, R L Han, Z J Li, J Geng, Y D Tian, R R Jiang, J P Wu, X T Kang
Research on the identity of genes and their relationship with traits of economic importance in chickens could assist in the selection of poultry. In this study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to detect single-nucleotide polymorphisms (SNPs) in the flanking region of the ASB15 gene by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). One SNP of -1271 C>T in 5' flanking region of the chicken ASB15 gene and two SNPs of the 10618 A>G and 10716 G>A in 3' flanking region were identified...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715465/comparison-of-cryopreservation-protocols-single-and-two-steps-and-thawing-fast-and-slow-for-canine-sperm
#8
COMPARATIVE STUDY
Maíra M Brito, Cristina F Lúcio, Daniel S R Angrimani, João Diego A Losano, Andressa Dalmazzo, Marcílio Nichi, Camila I Vannucchi
In addition to the existence of several cryopreservation protocols, no systematic research has been carried out in order to confirm the suitable protocol for canine sperm. This study aims to assess the effect of adding 5% glycerol during cryopreservation at 37°C (one-step) and 5°C (two-steps), in addition of testing two thawing protocols (37°C for 30 seconds, and 70°C for 8 seconds). We used 12 sperm samples divided into four experimental groups: Single-Step - Slow Thawing Group; Two-Step - Slow Thawing Group; Single-Step - Fast Thawing Group; and Two-Step - Fast Thawing Group...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715461/correlation-of-the-a-fabp-gene-polymorphism-and-mrna-expression-with-intramuscular-fat-content-in-three-yellow-chicken-and-hetian-black-chicken
#9
Yong Wang, Hongwei Chen, Diangang Han, Ying Chen, Gemingguli Muhatai, Tursunjan Kurban, Jinming Xing, Jianzhong He
The adipocyte-type fatty acid-binding protein (A-FABP) is considered a candidate gene for fat metabolism; thus, it affects fat deposition in chickens. The present study was designed to examine the polymorphism and mRNA abundance of the A-FABP gene with intramuscular fat (IMF) in the pectoralis muscles (PM) and leg muscles (LM) of Three-yellow Chicken (TYC) and Hetian-black Chicken (HTBC). In total, 60 TYCs and 60 HTBCs were sacrificed using exsanguination at market age. The IMF contents of the PM and LM in the HTBC were significantly higher than those in the TYC...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715460/bovine-sex-determining-region-y-cloning-optimized-expression-and-purification
#10
Bijan Soleymani, Sayed Hassan Hafezian, Ghodratollah Rahimi Mianji, Kamran Mansouri, Broomand Chaharaein, Ahmad Tajehmiri, Mehdi Sharifi Tabar, Ali Mostafaie
Sex determining region Y gene (SRY) is located on Y chromosome and encodes a protein with 229 amino acids. In this study, ORF region of SRY with a length of 690 bp was synthesized using PCR and ligated to pET28a (+), then transformed in E.coli DH5α. E.coli BL21 (DE3) strain was chosen to express recombinant bovine SRY protein. A set of optimization steps was taken including different concentrations of IPTG, glucose, and temperatures at differed incubation times after the induction. Results showed that temperature points and different concentrations of IPTG and glucose had a significant effect (p < 0...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27715456/sequence-based-structural-characterization-and-genetic-diversity-analysis-of-full-length-tlr4-cds-in-crossbred-and-indigenous-cattle
#11
Chinmoy Mishra, Subodh Kumar, Arvind Asaram Sonwane, H M Yathish, Rajni Chaudhary
The exploration of candidate genes for immune response in cattle may be vital for improving our understanding regarding the species specific response to pathogens. Toll-like receptor 4 (TLR4) is mostly involved in protection against the deleterious effects of Gram negative pathogens. Approximately 2.6 kb long cDNA sequence of TLR4 gene covering the entire coding region was characterized in two Indian milk cattle (Vrindavani and Tharparkar). The phylogenetic analysis confirmed that the bovine TLR4 was apparently evolved from an ancestral form that predated the appearance of vertebrates, and it is grouped with buffalo, yak, and mithun TLR4s...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27575333/gene-cloning-and-expression-of-cellulase-of-bacillus-amyloliquefaciens-isolated-from-the-cecum-of-goose
#12
Linghong Sun, Jiangyan Cao, Ying Liu, Junjie Wang, Panpan Guo, Zaigui Wang
A kind of bacteria secreting cellulase and showing probiotic attributes was isolated from the cecum of goose and identified as Bacillus amyloliquefaciens by analysis of 16S rRNA gene sequence and named as B. amyloliquefaciens S1. In vitro assays, the enzymatic activity of the strain was determined by the reducing-sugar method, and the proper culture conditions of producing cellulase and some properties of the cellulase were investigated. The cultural mixture of the bacteria had a high cellulase activity of 1...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27484003/expression-profiling-of-activin-type-iib-receptor-during-ontogeny-in-broiler-and-indigenous-chicken
#13
Pothana Boyina Guru Vishnu, Tarun K Bhattacharya, Pushpendra Kumar, R N Chaterjee, G V P P S Ravi Kumar, Chandan Paswan, Dushyanth Kotha Reddy, Athe Rajendra Prasad
Augmenting the meat production is among the primary breeding objective of genetic selection programs in poultry production. However, the knowledge about the expression of genes regulating muscle growth at the molecular level is inadequate. Activin type IIB receptor (ACTRIIB) has been reported to play vital role in the negative regulation of muscle growth by binding to multiple members of transforming growth factor-β superfamily. The present investigation was carried out to comprehend the trend of ACTRIIB messenger RNA in pectoralis major muscle during embryonic (E5-20) and post embryonic age (days 1, 14, 28, and 42) in both Control Broiler (CB) and Aseel by using Real-time PCR...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27385409/polypeptides-from-the-skin-of-rana-chensinensis-exert-the-antioxidant-and-antiapoptotic-activities-on-hacat-cells
#14
Xin Zhang, Yunyun Cheng, Yang Yang, Songcai Liu, Hui Shi, Chao Lu, Siming Li, Linyan Nie, Dan Su, Xuming Deng, Kexiang Ding, Linlin Hao
Studies have shown that frog skin secretes many types of peptides that are good for human skin. In this study, acid and enzymatic extracts of Rana skin peptides (acid/enzymatic Rana skin peptides, ARPs/ERPs) were obtained. The chemical and physical properties of the ARPs and ERPs were identified through UV scanning, HGLC, FRIT, and MS. MTS and flow cytometry were used to test the proproliferative and antiapoptotic effects of the ARPs and ERPs on human immortalized keratinocytes (HaCaT cells). To elucidate the antiapoptotic mechanisms, the mRNA and protein levels of EGF (epidermal growth factor, which enhances stimulation of cellular proliferation in both cells and epithelial tissues) and caspase-3 were evaluated using quantitative RT-PCR...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27385225/synthesis-of-recombinant-p48-of-mycoplasma-agalactiae-by-site-directed-mutagenesis-and-its-immunological-characterization
#15
Pawanjit Singh Cheema, Satparkash Singh, S Kathiresan, Ramesh Kumar, Vandna Bhanot, Vijendra Pal Singh
Contagious agalactia caused by Mycoplasma agalactiae is an economically important disease of sheep and goats and has been prevalent worldwide including India. The present study was undertaken to evaluate the membrane protein P48 of M. agalactiae for specific diagnosis of disease. For this, p48 gene of the organism was amplified by PCR and subjected to site directed mutagenesis to convert three TGA codons to TGG's and, subsequently, cloned into prokaryotic expression vector pPRO EX HTb. Purified recombinant P48 protein reacted to anti-P48 serum in western blotting, which confirmed its immunogenic nature...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27379566/expression-of-apoptotic-and-antioxidant-enzyme-genes-in-sheep-oocytes-and-in-vitro-produced-embryos
#16
Ashish Mishra, Ippala Janardhan Reddy, Paluru Subramanyam Parameswara Gupta, Sukanta Mondal
The present study was to find out the expression pattern and relative expression level of apoptotic (Bcl2, Bax, Casp3, and PCNA) and antioxidant enzyme [(GPx, Cu/Zn-SOD (SOD1) and Mn-SOD (SOD2)] genes in sheep oocytes and developing embryos produced in vitro by conventional RT-PCR and real time qPCR, respectively. Different developmental stages of embryos were produced in vitro from oocytes collected from local slaughter house ovaries. RT-PCR amplicons showed expression of Bcl2 and PCNA in all stages except at morula...
January 2, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/27874312/momilactone-b-inhibits-ketosis-in-vitro-by-regulating-the-angptl3-lpl-pathway-and-inhibiting-hmgcs2
#17
Dong Young Kang, Nipin S P, Pramod Darvin, Youn Hee Joung, Hyo Joo Byun, Chang Hee Do, Kyung Do Park, Mi Na Park, Kwang Hyun Cho, Young Mok Yang
Ketogenesis is the production of ketone bodies, which provide energy when the body lacks glucose. Under ketogenic conditions, the body switches from primarily carbohydrate to fat metabolism to maintain energy balance. However, accumulation of high levels of ketone bodies in the blood results in ketosis. Treating ketosis with natural substances is preferable, because they are unlikely to cause side-effects. Momilactone B is an active compound isolated from Korean rice. Based on previous studies, we hypothesized that momilactone B could inhibit ketosis...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27874309/abundance-and-characterization-of-perfect-microsatellites-on-the-cattle-y-chromosome
#18
Zhi-Jie Ma
Microsatellites or simple sequence repeats (SSRs) are found in most organisms and play an important role in genomic organization and function. To characterize the abundance of SSRs (1-6 base-pairs [bp]) on the cattle Y chromsome, the relative frequency and density of perfect or uninterrupted SSRs based on the published Y chromosome sequence were examined. A total of 17,273 perfect SSRs were found, with total length of 324.78 kb, indicating that approximately 0.75% of the cattle Y chromosome sequence (43.30 Mb) comprises perfect SSRs, with an average length of 18...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854178/porcine-cloned-embryos-reconstructed-with-the-cell-nuclei-of-tetraploid-m-phase-fibroblast-cells-can-restore-normal-diploidy-at-the-blastocyst-stage
#19
Q Zhao, Y G Qiu, J T Tian, C S Wang, T Z An
The cell cycle of donor cells as a major factor that affects cloning efficiency remains debatable. G2/M phase cells as a donor can successfully produce cloned animals, but a minimal amount is known regarding nuclear remodeling events. In this study, porcine fetal fibroblasts (PFFs) were carefully synchronized at G1 or M phase as donor cells. Most of the cloned embryos reconstructed from PFFs at G1 (G1-embryos) or M (M-embryos) phase formed a pronucleus-like nucleus (PN) within 6-h post fusion (hpf), but the M-embryos formed PN earlier than the G1-embryos did...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854162/endometritis-increases-pro-inflammatory-cytokines-in-follicular-fluid-and-cervico-vaginal-mucus-in-the-buffalo-cow
#20
Jones Boby, Harendra Kumar, Harihar Prasad Gupta, Mustapha Hussain Jan, Sanjay Kumar Singh, Manas Kumar Patra, Sukdeb Nandi, Asha Abraham, Narayanan Krishnaswamy
Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample collection. The present study describes the results of two different experiments. The first experiment was conducted to investigate the influence of endometritis on the proinflammatory cytokines of follicular fluid based on the reproductive tracts of buffalo collected at a slaughter house Buffalo genitalia were categorized into purulent endometritis (PE), cytological endometritis (CE), and non-endometritis (NE) based on the white-side test and endometrial cytology, respectively (n = 14/group)...
November 17, 2016: Animal Biotechnology
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