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Animal Biotechnology

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https://www.readbyqxmd.com/read/27874312/momilactone-b-inhibits-ketosis-in-vitro-by-regulating-the-angptl3-lpl-pathway-and-inhibiting-hmgcs2
#1
Dong Young Kang, Nipin S P, Pramod Darvin, Youn Hee Joung, Hyo Joo Byun, Chang Hee Do, Kyung Do Park, Mi Na Park, Kwang Hyun Cho, Young Mok Yang
Ketogenesis is the production of ketone bodies, which provide energy when the body lacks glucose. Under ketogenic conditions, the body switches from primarily carbohydrate to fat metabolism to maintain energy balance. However, accumulation of high levels of ketone bodies in the blood results in ketosis. Treating ketosis with natural substances is preferable, because they are unlikely to cause side-effects. Momilactone B is an active compound isolated from Korean rice. Based on previous studies, we hypothesized that momilactone B could inhibit ketosis...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27874309/abundance-and-characterization-of-perfect-microsatellites-on-the-cattle-y-chromosome
#2
Zhi-Jie Ma
Microsatellites or simple sequence repeats (SSRs) are found in most organisms and play an important role in genomic organization and function. To characterize the abundance of SSRs (1-6 base-pairs [bp]) on the cattle Y chromsome, the relative frequency and density of perfect or uninterrupted SSRs based on the published Y chromosome sequence were examined. A total of 17,273 perfect SSRs were found, with total length of 324.78 kb, indicating that approximately 0.75% of the cattle Y chromosome sequence (43.30 Mb) comprises perfect SSRs, with an average length of 18...
November 22, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854178/porcine-cloned-embryos-reconstructed-with-the-cell-nuclei-of-tetraploid-m-phase-fibroblast-cells-can-restore-normal-diploidy-at-the-blastocyst-stage
#3
Q Zhao, Y G Qiu, J T Tian, C S Wang, T Z An
The cell cycle of donor cells as a major factor that affects cloning efficiency remains debatable. G2/M phase cells as a donor can successfully produce cloned animals, but a minimal amount is known regarding nuclear remodeling events. In this study, porcine fetal fibroblasts (PFFs) were carefully synchronized at G1 or M phase as donor cells. Most of the cloned embryos reconstructed from PFFs at G1 (G1-embryos) or M (M-embryos) phase formed a pronucleus-like nucleus (PN) within 6-h post fusion (hpf), but the M-embryos formed PN earlier than the G1-embryos did...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854162/endometritis-increases-pro-inflammatory-cytokines-in-follicular-fluid-and-cervico-vaginal-mucus-in-the-buffalo-cow
#4
Jones Boby, Harendra Kumar, Harihar Prasad Gupta, Mustapha Hussain Jan, Sanjay Kumar Singh, Manas Kumar Patra, Sukdeb Nandi, Asha Abraham, Narayanan Krishnaswamy
Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample collection. The present study describes the results of two different experiments. The first experiment was conducted to investigate the influence of endometritis on the proinflammatory cytokines of follicular fluid based on the reproductive tracts of buffalo collected at a slaughter house Buffalo genitalia were categorized into purulent endometritis (PE), cytological endometritis (CE), and non-endometritis (NE) based on the white-side test and endometrial cytology, respectively (n = 14/group)...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854153/genetic-factors-affecting-pork-quality-halothane-and-rendement-napole-genes
#5
Ramon Cesar D Salas, Claro N Mingala
The most common pork quality problems are pale, soft, and exudative (PSE) and acid pork (AP). PSE is associated with the expression of recessive halothane (Hal) allele Hal(n). Recessive Hal pigs (Hal(nn)) have defective Ca(2+) release channels (CRC) or Ryanodine Receptors (RYR1) within the sarcoplasmic reticulum that allow uncontrolled release of Ca(2+) in response to stress. Abnormal lactic acid metabolism caused by stress prior to slaughter leads to the sudden drop in postmortem muscle pH producing the PSE pork...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27854152/identification-of-four-snps-in-lhb-gene-and-their-associations-with-sperm-qualities-of-chinese-buffaloes
#6
Yunyun Cheng, Jiaming Gu, Han Xue, Qiang Li, Mingming Liang, Nan Wang, Gang Wang, Qingyan Wu, Songcai Liu, Hao Yu, Jiabao Zhang, Linlin Hao
Luteinizing hormone beta polypeptide (LHB) gene has been considered important for sexual behavior and has associations with sperm quality. In this study, four SNPs (g.276 T>C, g.377A>C, g.401T>C, and g.412A>G) were detected in the LHB gene of 165 water buffaloes by direct sequencing and identification of overlap peaks, each of which was associated with at least one sperm quality trait of ejaculate volume, sperm concentration, post-thaw sperm motilities, and sperm abnormalities by chi-square analysis...
November 17, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27834588/generation-of-ggta1-mutant-pigs-by-direct-pronuclear-microinjection-of-crispr-cas9-plasmid-vectors
#7
Chin-Kai Chuang, Chien-Hong Chen, Chung-Ling Huang, Yu-Hsiu Su, Shu-Hui Peng, Tai-Yun Lin, Hao-Chih Tai, Tien-Shuh Yang, Ching-Fu Tu
This study was conducted to confirm that 1-site and 4-site ppU6-GGTA1-gRNA CRISPR vectors together with the pCX-Flag2-NLS1-Cas9-NLS2 plasmid can both generate KO pigs by direct pronuclear microinjection. In total, 41 and 53 fertilized eggs were microinjected on 1-site and 4-site strategies, respectively. The 1-site construction generated a litter of 8 piglets, and 2 were mono-allelic mutant (mMt). The injection of 4-site constructions resulted in one biallelic mutant (bMt) and one mMt piglet in a litter of 7...
November 11, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27824510/association-of-bf-rbp4-and-esr2-genotypes-with-litter-size-in-an-autochthonous-pig-population
#8
G P Laliotis, A Marantidis, M Avdi
: The aim of the present study was to determine any potential association of the BF, RBP4, and ESR2 genes with reproduction traits in an autochthonous Greek pig population. The PCR-RFLP methodology was implemented for genotyping purposes of the examined genes. No deviation from the Hardy-Weinberg equilibrium was observed for the examined loci, while the B allele noted to be the more frequent in all analyzed genes. In addition, sows with the AA genotype of BF gene found to produce significantly lower numbers of the total born piglets (TNB) and number of piglets born alive (TNA), while the respective BB genotype significantly exceeded in TNB and NBA traits compared to the other two genotypes (P < 0...
November 8, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27813832/single-nucleotide-polymorphisms-in-il8-and-tlr4-genes-as-candidates-for-digital-dermatitis-resistance-susceptibility-in-holstein-cattle
#9
El-Sayed El-Shafaey, Ahmed Ateya, Hazem Ramadan, Rasha Saleh, Yousef Elseady, Eman Abo El Fadl, Sabry El-Khodery
Relatedness between single nucleotide polymorphisms in IL8 and TLR4 genes and digital dermatitis resistance/susceptibility was investigated in seventy Holstein dairy cows. Animals were assigned into two groups, affected group (n = 35) and resistant group (n = 35) based on clinical signs and previous history of farm clinical records. Blood samples were collected for DNA extraction to ampliy fragments of 267-bp and 382-bp for IL8 and TLR4 genes, respectively. PCR-DNA sequencing revealed three SNPs in each of IL8 and TLR4 genes...
November 4, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27791596/antiviral-compounds-against-nucleocapsid-protein-of-porcine-epidemic-diarrhea-virus
#10
Nipaporn Deejai, Yaowaluck Maprang Roshorm, Anchanee Kubera
Porcine epidemic diarrhea (PED) is a severe diarrhea disease in swine that is caused by porcine epidemic diarrhea virus (PEDV). Nucleocapsid (N) protein is the RNA-binding protein of PEDV, which plays an important role for virus life cycle. The aim of this research was to screen and characterize the compounds that could inhibit the activity of PEDV N protein. The gene encoding PEDV N protein obtained from PEDV Thai isolate was cloned and expressed in E. coli. Its amino acid sequence was employed to generate the three dimensional structure by homology modeling...
October 28, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27791476/polymorphisms-of-the-toll-like-receptor-2-of-goats-capra-hircus-may-be-associated-with-somatic-cell-count-in-milk
#11
Christina T Ruiz-Rodriguez, Jessica R Brandt, Ryan Oliverio, Yasuko Ishida, Noa Guedj, Edgar F Garrett, Gila Kahila Bar-Gal, Nikolas Nikolaidis, Felipe C Cardoso, Alfred L Roca
Toll-like receptor 2 (TLR2) plays an important role in recognition by the innate immune system of Gram-positive bacteria. As Gram-positive bacteria cause mastitis, we examined variations in the region of the TLR2 gene that codes for the extracellular domain. Samples of forty goats from a single dairy herd were collected, half with low SCC (≤200,000 cells/mL), and half with higher SCC. Two synonymous single nucleotide polymorphisms (SNPs) were identified: 840G > A and 1083A > G. One nonsynonymous SNP 739G > A was identified...
October 28, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27736301/polymorphisms-of-flanking-region-of-the-asb15-gene-and-their-associations-with-performance-traits-in-chicken
#12
Y C Wang, R L Han, Z J Li, J Geng, Y D Tian, R R Jiang, J P Wu, X T Kang
Research on the identity of genes and their relationship with traits of economic importance in chickens could assist in the selection of poultry. In this study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to detect single-nucleotide polymorphisms (SNPs) in the flanking region of the ASB15 gene by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). One SNP of -1271 C>T in 5' flanking region of the chicken ASB15 gene and two SNPs of the 10618 A>G and 10716 G>A in 3' flanking region were identified...
October 13, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27623989/molecular-cloning-and-expression-profile-of-toll-like-receptor-3-from-an-indian-coldwater-fish-schizothorax-richardsonii-gray
#13
Kiran Belwal, Dimpal Thakuria, Vikas Dighe, Veena Pande, Amit Pande
The innate immune system recognizes pathogens through pattern recognition receptors (PRRs), and toll-like receptors (TLRs) are one of the most important PRRs. TLR3 is a unique member of TLR family that recognizes double-stranded RNA (dsRNA), a viral replication intermediate. There is a variation in its response among diverse fish species toward the same stimulants. We identified and cloned TLR3 from Indian snow trout, Schizothorax richardsonii and carried out its expression analysis in un-induced and poly (I:C) challenged fish...
September 13, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27623936/identification-of-differentially-expressed-genes-and-pathways-for-myofiber-characteristics-in-soleus-muscles-between-chicken-breeds-differing-in-meat-quality
#14
Y F Du, Q L Ding, Y M Li, W R Fang
In the modern chicken industry, fast-growing broilers have undergone strong artificial selection for muscle growth, which has led to remarkable phenotypic variations compared with slow-growing chickens. However, the molecular mechanism underlying these phenotypes differences remains unknown. In this study, a systematic identification of candidate genes and new pathways related to myofiber development and composition in chicken Soleus muscle (SOL) has been made using gene expression profiles of two distinct breeds: Qingyuan partridge (QY), a slow-growing Chinese breed possessing high meat quality and Cobb 500 (CB), a commercial fast-growing broiler line...
September 13, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565877/editorial-board-eov
#15
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565876/novel-polymorphic-microsatellite-markers-for-panulirus-ornatus-and-their-cross-species-primer-amplification-in-panulirus-homarus
#16
Madjid Delghandi, Hasifa Afzal, Manal Saif Nasser Al Hinai, Rafaida Dhuhai Gharib Al-Breiki, Dean R Jerry, Hoc Tan Dao
Polymorphic microsatellite loci were isolated for Panulirus ornatus using 454 GS-FLX Titanium pyrosequencing. Fifteen markers containing perfect di-, tri-, tetra-, and penta-nucleotide motifs were consistently co-amplified in five multiplexes in a panel of 91 randomly selected samples. Observed number of alleles varied from 2 to 14 per locus. Observed and expected heterozygosity ranged from 0.090 to 0.79 and 0.08 to 0.87, respectively. Ten loci deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction...
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565875/expression-profiling-of-innate-immune-genes-in-milk-somatic-cells-during-subclinical-mastitis-in-crossbred-dairy-cows
#17
A Karthikeyan, G Radhika, T V Aravindhakshan, K Anilkumar
Innate immune mechanism plays a key role in mammary defense, from recognition of pathogens to activation of nonspecific and specific immunity involved in elimination of pathogens. Expression profiles of innate immune response genes namely Toll like receptor 2 (TLR-2), Peptidoglycan recognition protein 1 (PGLYRP-1), Interleukin 8 receptor (IL-8 R), L-Selectin (SELL), and Osteopontin (OPN) in milk somatic cells of subclinical mastitis (SCM) affected crossbred cows were investigated under this study at transcript level using quantitative real time polymerase chain reaction (qRT-PCR)...
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565874/expression-regulation-and-functional-characterization-of-fst-gene-in-porcine-granulosa-cells
#18
QuanYong Zhou, MingChun Wan, QiPeng Wei, QiongLi Song, LiGen Xiong, JunHong Huo, JiangNan Huang
Proliferation, differentiation, and estrogen secretion of granulosa cells are the key factors affecting the estrous after weaning in sows. The objective of this study was to evaluate the expression of Follistatin (FST) in the ovary of Xiushui Hang and Duroc sows at weaning and estrus, the effect of FSH on transcript abundance of FST gene in granulosa cells and the role of FST gene in the weaning to estrus using siRNAs targeted to FST gene. In the present study, expression of the FST mRNA was evaluated by real time PCR...
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565873/role-of-phosphotyrosine-interaction-domain-containing-1-in-porcine-intramuscular-preadipocyte-proliferation-and-differentiation
#19
Xiaoling Chen, Yanliu Luo, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Phosphotyrosine interaction domain containing 1 (PID1), a recently identified gene involved in obesity-associated insulin resistance, plays an important role in fat deposition. However, its effect on porcine intramuscular preadipocyte proliferation and differentiation remains poorly understood. In this study, the plasmid pcDNA3.1(+)-pPID1 was transfected into porcine intramuscular preadipocytes with Lipofectamine 3000 reagent to over-express porcine PID1 (pPID1). Over-expression of pPID1 significantly promoted porcine intramuscular preadipocyte proliferation...
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27565872/effect-of-genetic-variations-within-the-i-mfa-gene-on-the-growth-traits-of-chinese-cattle
#20
Yong-Zhen Huang, Jing-Jing Li, Chun-Lei Zhang, Xing-Tang Fang, Xian-Yong Lan, Xing-Lei Qi, Hong Chen
I-mfa (inhibitor of the MyoD family a) is a transcription modulator that binds to MyoD family members and inhibits their transcriptional activities. It is highly expressed in the sclerotome and plays an important role in the patterning of the somite early in development. In this study, the polymorphisms of the bovine I-mfa gene were detected by polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and DNA pool sequencing methods in 541 individuals from three Chinese cattle breeds...
October 2016: Animal Biotechnology
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