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Journal of the American Society for Mass Spectrometry

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https://www.readbyqxmd.com/read/29450858/in-source-reduction-of-disulfide-bonded-peptides-monitored-by-ion-mobility-mass-spectrometry
#1
Bradley B Stocks, Jeremy E Melanson
Many peptides with antimicrobial activity and/or therapeutic potential contain disulfide bonds as a means to enhance stability, and their quantitation is often performed using electrospray ionization mass spectrometry (ESI-MS). Disulfides can be reduced during ESI under commonly used instrument conditions, which has the potential to hinder accurate peptide quantitation. We demonstrate that this in-source reduction (ISR) is predominantly observed for peptides infused from acidic solutions and subjected to elevated ESI voltages (3-4 kV)...
February 15, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29435768/a-multifaceted-mass-spectrometric-method-to-probe-feeding-related-neuropeptide-changes-in-callinectes-sapidus-and-carcinus-maenas
#2
Yuzhuo Zhang, Kellen DeLaney, Limei Hui, Junhua Wang, Robert M Sturm, Lingjun Li
Food intake is regulated by various neuromodulators, including numerous neuropeptides. However, it remains elusive at the molecular and cellular level as to how these important chemicals regulate internal processes and which regions of the neuronal organs are responsible for regulating the behavior. Here we report a comparative neuropeptidomic analysis of the brain and pericardial organ (PO) in response to feeding in two well-studied crustacean physiology model organisms, Callinectes sapidus and Carcinus maenas, using mass spectrometry (MS) techniques...
February 12, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29427066/ion-neutral-clustering-of-bile-acids-in-electrospray-ionization-across-uplc-flow-regimes
#3
Patrick Brophy, Corey D Broeckling, James Murphy, Jessica E Prenni
Bile acid authentic standards were used as model compounds to quantitatively evaluate complex in-source phenomenon on a UPLC-ESI-TOF-MS operated in the negative mode. Three different diameter columns and a ceramic-based microfluidic separation device were utilized, allowing for detailed descriptions of bile acid behavior across a wide range of flow regimes and instantaneous concentrations. A custom processing algorithm based on correlation analysis was developed to group together all ion signals arising from a single compound; these grouped signals produce verified compound spectra for each bile acid at each on-column mass loading...
February 9, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29417494/maldi-mass-spectral-imaging-of-bile-acids-observed-as-deprotonated-molecules-and-proton-bound-dimers-from-mouse-liver-sections
#4
Ignacy Rzagalinski, Nadine Hainz, Carola Meier, Thomas Tschernig, Dietrich A Volmer
Bile acids (BAs) play two vital roles in living organisms, as they are involved in (1) the secretion of cholesterol from liver, and (2) the lipid digestion/absorption in the intestine. Abnormal bile acid synthesis or secretion can lead to severe liver disorders. Even though there is extensive literature on the mass spectrometric determination of BAs in biofluids and tissue homogenates, there are no reports on the spatial distribution in the biliary network of the liver. Here, we demonstrate the application of high mass resolution/mass accuracy matrix-assisted laser desorption/ionization (MALDI)-Fourier-transform ion cyclotron resonance (FTICR) to MS imaging (MSI) of BAs at high spatial resolutions (pixel size, 25 μm)...
February 7, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29404970/fungal-secretome-analysis-via-pepsavi-ms-identification-of-the-bioactive-peptide-kp4-from-ustilago-maydis
#5
Christine L Kirkpatrick, Nicole C Parsley, Tessa E Bartges, Madeline E Cooke, Wilaysha S Evans, Lilian R Heil, Thomas J Smith, Leslie M Hicks
Fungal secondary metabolites represent a rich and largely untapped source for bioactive molecules, including peptides with substantial structural diversity and pharmacological potential. As methods proceed to take a deep dive into fungal genomes, complimentary methods to identify bioactive components are required to keep pace with the expanding fungal repertoire. We developed PepSAVI-MS to expedite the search for natural product bioactive peptides and herein demonstrate proof-of-principle applicability of the pipeline for the discovery of bioactive peptides from fungal secretomes via identification of the antifungal killer toxin KP4 from Ustilago maydis P4...
February 5, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29404969/neutral-loss-scan-based-strategy-for-integrated-identification-of-amorfrutin-derivatives-new-peroxisome-proliferator-activated-receptor-gamma-agonists-from-amorpha-fruticosa-by-uplc-qqq-ms-ms-and-uplc-q-tof-ms
#6
Chu Chen, Ying Xue, Qing-Miao Li, Yan Wu, Jian Liang, Lin-Sen Qing
Amorfrutins with a 2-hydroxybenzoic acid core structure are promising natural PPARγ agonists with potent antidiabetic activity. Owing to the complex matrix and low concentration in botanical material, the identification of unknown amorfrutins remains a challenge. In the present study, a combined application of UPLC-Q-TOF-MS and UPLC-QqQ-MS was developed to discover unknown amorfrutins from fruits of Amorpha fruticosa. First, reference compounds of amorfrutin A (AA), amorfrutin B (AB), and 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (AC) were analyzed using UPLC-Q-TOF-MS to reveal the characteristic fragment ions and the possible neutral loss...
February 5, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29404968/aerosol-vacuum-assisted-plasma-ionization-aero-vapi-coupled-to-ion-mobility-mass-spectrometry
#7
Sandra L Blair, Nga L Ng, Stephen C Zambrzycki, Anyin Li, Facundo M Fernández
In this communication, we report on the real-time analysis of organic aerosol particles by Vacuum-assisted Plasma Ionization-Mass Spectrometry (Aero-VaPI-MS) using a home-built VaPI ion source coupled to a Synapt G2-S HDMS ion mobility-mass spectrometry (IM-MS) system. Standards of organic molecules of interest in prebiotic chemistry were used to generate aerosols. Monocaprin and decanoic acid aerosol particles were successfully detected in both the positive and negative ion modes, respectively. A complex aerosol mixture of different sizes of polymers of L-malic acid was also examined through ion mobility (IM) separations, resulting in the detection of polymers of up to eight monomeric units...
February 5, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29392686/ionization-mechanism-of-positive-ion-nitrogen-direct-analysis-in-real-time
#8
Liguo Song, Wei Chean Chuah, Xinyi Lu, Edward Remsen, John E Bartmess
Nitrogen can be an inexpensive alternative to helium used by direct analysis in real time (DART), especially in consideration of the looming helium shortage. Therefore, the ionization mechanism of positive-ion N2 DART has been systematically investigated. Our experiments suggest that a range of metastable nitrogen species with a variety of internal energies existed and all of them were less energetic than metastable helium atoms. However, compounds with ionization energies (IE) equal to or lower than 10.2 eV (all organic compounds except the extremely small ones) can be efficiently ionized...
February 1, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29392685/a-simple-sonication-improves-protein-signal-in-matrix-assisted-laser-desorption-ionization-imaging
#9
Li-En Lin, Pin-Rui Su, Hsin-Yi Wu, Cheng-Chih Hsu
Proper matrix application is crucial in obtaining high quality matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Solvent-free sublimation was essentially introduced as an approach of homogeneous coating that gives small crystal size of the organic matrix. However, sublimation has lower extraction efficiency of analytes. Here, we present that a simple sonication step after the hydration in standard sublimation protocol significantly enhances the sensitivity of MALDI MSI. This modified procedure uses a common laboratory ultrasonicator to immobilize the analytes from tissue sections without noticeable delocalization...
February 1, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29392684/determining-the-effect-of-catechins-on-sod1-conformation-and-aggregation-by-ion-mobility-mass-spectrometry-combined-with-optical-spectroscopy
#10
Bing Zhao, Xiaoyu Zhuang, Zifeng Pi, Shu Liu, Zhiqiang Liu, Fengrui Song
The aggregation of Cu,Zn-superoxide dismutase (SOD1) plays an important role in the etiology of amyotrophic lateral sclerosis (ALS). For the disruption of ALS progression, discovering new drugs or compounds that can prevent SOD1 aggregation is important. In this study, ESI-MS was used to investigate the interaction of catechins and SOD1. The noncovalent complex of catechins that interact with SOD1 was found and retained in the gas phase under native ESI-MS condition. The conformation changes of SOD1 after binding with catechins were also explored via traveling wave ion mobility (IM) spectrometry...
February 1, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29388167/surface-induced-dissociation-coupled-with-high-resolution-mass-spectrometry-unveils-heterogeneity-of-a-211-kda-multicopper-oxidase-protein-complex
#11
Mowei Zhou, Jing Yan, Christine A Romano, Bradley M Tebo, Vicki H Wysocki, Ljiljana Paša-Tolić
Manganese oxidation is an important biogeochemical process that is largely regulated by bacteria through enzymatic reactions. However, the detailed mechanism is poorly understood due to challenges in isolating and characterizing these unknown enzymes. A manganese oxidase, Mnx, from Bacillus sp. PL-12 has been successfully overexpressed in active form as a protein complex with a molecular mass of 211 kDa. We have recently used surface induced dissociation (SID) and ion mobility-mass spectrometry (IM-MS) to release and detect folded subcomplexes for determining subunit connectivity and quaternary structure...
January 31, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29380268/rapid-characterization-of-insulin-modifications-and-sequence-variations-by-proteinase-k-digestion-and-uhplc-esi-ms
#12
Rong-Sheng Yang, Weijuan Tang, Huaming Sheng, Fanyu Meng
Discovery of novel insulin analogs as therapeutics has remained an active area of research. Compared with native human insulin, insulin analog molecules normally incorporate either covalent modifications or amino acid sequence variations. From the drug discovery and development perspective, methods for efficient and detailed characterization of these primary structural changes are very important. In this report, we demonstrate that proteinase K digestion coupled with UPLC-ESI-MS analysis provides a simple and rapid approach to characterize the modifications and sequence variations of insulin molecules...
January 29, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29372554/an-orbital-trap-mass-analyzer-using-a-hybrid-magnetic-electric-field-a-simulation-study
#13
Chongsheng Xu, Fangling Wu, Li Ding, Chuan-Fan Ding
An orbital ion trap mass analyzer employing hybrid magnetic-electric field was designed and simulated. The trap has a rotational symmetrical structure and the hybrid trapping field was created in a toroidal space between 12 pairs of sector detection electrodes. Ion injection and ion orbital motion inside the trap were simulated using SIMION 8.1 with a user Lua program, and the required electric and magnetic field were investigated. The image charge signal can be picked up by the 12 pairs of detection electrodes and the mass resolution was evaluated using FFT...
January 25, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29372553/using-gas-phase-reactions-of-hexamethylene-triperoxide-diamine-hmtd-to-improve-detection-in-mass-spectrometry
#14
Kevin Colizza, Alexander Yevdokimov, Lindsay McLennan, James L Smith, Jimmie C Oxley
Our efforts to lower the detection limits of hexamethylene triperoxide diamine (HMTD) have uncovered previously unreported gas-phase reactions of primary and secondary amines with one of the six methylene carbons. The reaction occurs primarily in the atmospheric pressure chemical ionization (APCI) source and is similar to the behavior of alcohols with HMTD [1]. However, unlike alcohols, the amine reaction conserves the hydrogen peroxide on the intact product. Furthermore, with or without amines, HMTD is oxidized to tetramethylene diperoxide diamine dialdehyde (TMDDD) in a temperature-dependent fashion in the APCI source...
January 25, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29372552/characterization-of-disulfide-linked-peptides-using-tandem-mass-spectrometry-coupled-with-automated-data-analysis-software
#15
Zhidan Liang, Kenneth N McGuinness, Alejandro Crespo, Wendy Zhong
Disulfide bond formation is critical for maintaining structure stability and function of many peptides and proteins. Mass spectrometry has become an important tool for the elucidation of molecular connectivity. However, the interpretation of the tandem mass spectral data of disulfide-linked peptides has been a major challenge due to the lack of appropriate tools. Developing proper data analysis software is essential to quickly characterize disulfide-linked peptides. A thorough and in-depth understanding of how disulfide-linked peptides fragment in mass spectrometer is a key in developing software to interpret the tandem mass spectra of these peptides...
January 25, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29372551/neuropeptide-mapping-of-dimmed-cells-of-adult-drosophila-brain
#16
Max Diesner, Reinhard Predel, Susanne Neupert
Neuropeptides are structurally highly diverse messenger molecules that act as regulators of many physiological processes such as development, metabolism, reproduction or behavior in general. Differentiation of neuropeptidergic cells often corresponds with the presence of the transcription factor DIMMED. In the central nervous system of the fruit fly Drosophila melanogaster, DIMMED commonly occurs in neuroendocrine neurons that release peptides as neurohormones but also in interneurons with complex branching patterns...
January 25, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29372550/analysis-of-compounds-dissolved-in-nonpolar-solvents-by-electrospray-ionization-on-conductive-nanomaterials
#17
Bing Xia, Yuanji Gao, Baocheng Ji, Fengwei Ma, Lisheng Ding, Yan Zhou
Electrospray ionization mass spectrometry (ESI-MS) technique has limitations in analysis of compounds that are dissolved in nonpolar solvents. In this study, ambient ionization of compounds in solvents that are not "friendly" to electrospray ionization, such as n-hexane, is achieved by conductive nanomaterials spray ionization (CNMSI) on nanomaterial emitters, including carbon nanotubes paper and mesodendritic silver covered metal, which applies high voltages to emitters made of these materials without the assistance of polar solvents...
January 25, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29349714/dbda-as-a-novel-matrix-for-the-analyses-of-small-molecules-and-quantification-of-fatty-acids-by-negative-ion-maldi-tof-ms
#18
Ling Ling, Ying Li, Sheng Wang, Liming Guo, Chunsheng Xiao, Xuesi Chen, Xinhua Guo
Matrix interference ions in low mass range has always been a concern when using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze small molecules (<500 Da). In this work, a novel matrix, N1,N4-dibenzylidenebenzene-1,4-diamine (DBDA) was synthesized for the analyses of small molecules by negative ion MALDI-TOF MS. Notably, only neat ions ([M-H]-) of fatty acids without matrix interference appeared in the mass spectra and the limit of detection (LOD) reached 0...
January 18, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29340959/matrix-assisted-and-or-laser-desorption-ionization-quadrupole-ion-trap-time-of-flight-mass-spectrometry-of-wo3-clusters-formation-in-gas-phase-nanodiamonds-fullerene-and-graphene-oxide-matrices
#19
Mayuri Vilas Ausekar, Ravi Madhukar Mawale, Pavel Pazdera, Josef Havel
The formation of W x O y+●/-● clusters in the gas phase was studied by laser desorption ionization (LDI) and matrix assisted laser desorption ionization (MALDI) of solid WO3. LDI produced (WO3) n+ ●/- ● (n = 1-7) clusters. In MALDI, when using nano-diamonds (NDs), graphene oxide (GO), or fullerene (C60) matrices, higher mass clusters were generated. In addition to (WO3) n-● clusters, oxygen-rich or -deficient species were found in both LDI and MALDI (with the total number of clusters exceeding one hundred ≈ 137)...
January 16, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29340958/robert-c-dunbar-june-26-1943-october-31-2017
#20
Victor Ryzhov, Jos Oomens
No abstract text is available yet for this article.
January 16, 2018: Journal of the American Society for Mass Spectrometry
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