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Journal of the American Society for Mass Spectrometry

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https://www.readbyqxmd.com/read/28631114/derivatization-of-dextran-for-multiply-charged-ion-formation-and-electrospray-ionization-time-of-flight-mass-spectrometric-analysis
#1
Jesus B Tapia, Hailey A J Hibbard, Melissa M Reynolds
We present the use of a simple, one-pot derivatization to allow the polysaccharide dextran to carry multiple positive charges, shifting its molecular weight distribution to a lower m/z range. We performed this derivatization because molecular weight measurements of polysaccharides by mass spectrometry are challenging because of their lack of readily ionizable groups. The absence of ionizable groups limits proton abstraction and suppresses proton adduction during the ionization process, producing mass spectra with predominantly singly charged metal adduct ions, thereby limiting the detection of large polysaccharides...
June 19, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28631113/fast-determination-of-ingredients-in-solid-pharmaceuticals-by-microwave-enhanced-in-source-decay-of-microwave-plasma-torch-mass-spectrometry
#2
Rui Su, Xinchen Wang, Changming Hou, Meiling Yang, Keke Huang, Huanwen Chen
Rapid qualitative and quantitative analysis of solid samples (e.g., pharmaceutical preparations) by using a small and low-resolution mass spectrometer without MS/MS function is still a challenge in ambient pressure ionization mass spectrometric analysis. Herein, a practically efficient method termed microwave-enhanced in-source decay (MEISD) using microwave plasma torch desorption ionization coupled with time-of-flight mass spectrometry (MPTDI-TOF MS) was developed for fast analysis of pharmaceutical tablets using a miniature TOF mass spectrometer without tandem mass function...
June 19, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28620847/faster-more-reproducible-desi-ms-for-biological-tissue-imaging
#3
Jocelyn Tillner, Vincen Wu, Emrys A Jones, Steven D Pringle, Tamas Karancsi, Andreas Dannhorn, Kirill Veselkov, James S McKenzie, Zoltan Takats
A new, more robust sprayer for desorption electrospray ionization (DESI) mass spectrometry imaging is presented. The main source of variability in DESI is thought to be the uncontrolled variability of various geometric parameters of the sprayer, primarily the position of the solvent capillary, or more specifically, its positioning within the gas capillary or nozzle. If the solvent capillary is off-center, the sprayer becomes asymmetrical, making the geometry difficult to control and compromising reproducibility...
June 15, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28616748/intact-transition-epitope-mapping-item
#4
Yelena Yefremova, Kwabena F M Opuni, Bright D Danquah, Hans-Juergen Thiesen, Michael O Glocker
Intact transition epitope mapping (ITEM) enables rapid and accurate determination of protein antigen-derived epitopes by either epitope extraction or epitope excision. Upon formation of the antigen peptide-containing immune complex in solution, the entire mixture is electrosprayed to translate all constituents as protonated ions into the gas phase. There, ions from antibody-peptide complexes are separated from unbound peptide ions according to their masses, charges, and shapes either by ion mobility drift or by quadrupole ion filtering...
June 14, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28593377/microms-a-python-platform-for-image-guided-mass-spectrometry-profiling
#5
Troy J Comi, Elizabeth K Neumann, Thanh D Do, Jonathan V Sweedler
Image-guided mass spectrometry (MS) profiling provides a facile framework for analyzing samples ranging from single cells to tissue sections. The fundamental workflow utilizes a whole-slide microscopy image to select targets of interest, determine their spatial locations, and subsequently perform MS analysis at those locations. Improving upon prior reported methodology, a software package was developed for working with microscopy images. microMS, for microscopy-guided mass spectrometry, allows the user to select and profile diverse samples using a variety of target patterns and mass analyzers...
June 7, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28593376/insight-into-signal-response-of-protein-ions-in-native-esi-ms-from-the-analysis-of-model-mixtures-of-covalently-linked-protein-oligomers
#6
Katharina Root, Yves Wittwer, Konstantin Barylyuk, Ulrike Anders, Renato Zenobi
Native ESI-MS is increasingly used for quantitative analysis of biomolecular interactions. In such analyses, peak intensity ratios measured in mass spectra are treated as abundance ratios of the respective molecules in solution. While signal intensities of similar-size analytes, such as a protein and its complex with a small molecule, can be directly compared, significant distortions of the peak ratio due to unequal signal response of analytes impede the application of this approach for large oligomeric biomolecular complexes...
June 7, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28589488/negative-electron-transfer-dissociation-sequencing-of-increasingly-sulfated-glycosaminoglycan-oligosaccharides-on-an-orbitrap-mass-spectrometer
#7
Franklin E Leach, Nicholas M Riley, Michael S Westphall, Joshua J Coon, I Jonathan Amster
The structural characterization of sulfated glycosaminoglycan (GAG) carbohydrates remains an important target for analytical chemists attributable to challenges introduced by the natural complexity of these mixtures and the defined need for molecular-level details to elucidate biological structure-function relationships. Tandem mass spectrometry has proven to be the most powerful technique for this purpose. Previously, electron detachment dissociation (EDD), in comparison to other methods of ion activation, has been shown to provide the largest number of useful cleavages for de novo sequencing of GAG oligosaccharides, but such experiments are restricted to Fourier transform ion cyclotron resonance mass spectrometers (FTICR-MS)...
June 6, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28585116/charge-mediated-compaction-and-rearrangement-of-gas-phase-proteins-a-case-study-considering-two-proteins-at-opposing-ends-of-the-structure-disorder-continuum
#8
Jacquelyn R Jhingree, Bruno Bellina, Kamila J Pacholarz, Perdita E Barran
Charge reduction in the gas phase provides a direct means of manipulating protein charge state, and when coupled to ion mobility mass spectrometry (IM-MS), it is possible to monitor the effect of charge on protein conformation in the absence of solution. Use of the electron transfer reagent 1,3-dicyanobenzene, coupled with IM-MS, allows us to monitor the effect of charge reduction on the conformation of two proteins deliberately chosen from opposite sides of the order to disorder continuum: bovine pancreatic trypsin inhibitor (BPTI) and beta casein...
June 5, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28577288/single-analyzer-precursor-ion-scans-in-a-linear-quadrupole-ion-trap-using-orthogonal-double-resonance-excitation
#9
Dalton T Snyder, R Graham Cooks
Reported herein is a simple method of performing single analyzer precursor ion scans in a linear quadrupole ion trap using orthogonal double resonance excitation. A first supplementary AC signal applied to the y electrodes is scanned through ion secular frequencies in order to mass-selectively excite precursor ions while, simultaneously, a second fixed-frequency AC signal is applied orthogonally on the x electrodes in order to eject product ions of selected mass-to-charge ratios towards the detector. The two AC signals are applied orthogonally so as to preclude the possibility of (1) inadvertently ejecting precursor ions into the detector, which results in artifact peaks, and (2) prevent beat frequencies on the x electrodes from ejecting ions off-resonance...
June 2, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28560565/a-novel-triethylphosphonium-charge-tag-on-peptides-synthesis-derivatization-and-fragmentation
#10
Nick DeGraan-Weber, Sarah A Ward, James P Reilly
Charge tagging is a peptide derivatization process that commonly localizes a positive charge on the N-terminus. Upon low energy activation (e.g., collision-induced dissociation or post-source decay) of charge tagged peptides, relatively few fragment ions are produced due to the absence of mobile protons. In contrast, high energy fragmentation, such as 157 nm photodissociation, typically leads to a series of a-type ions. Disadvantages of existing charge tags are that they can produce mobile protons or that they are undesirably large and bulky...
May 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28560564/an-electrospray-ionization-mass-spectrometry-study-on-the-in-vacuo-hetero-oligomers-formed-by-the-antimicrobial-peptides-surfactin-and-gramicidin-s
#11
Marina Rautenbach, N Maré Vlok, Hans A Eyéghé-Bickong, Marthinus J van der Merwe, Marietjie A Stander
It was previously observed that the lipopeptide surfactants in surfactin (Srf) have an antagonistic action towards the highly potent antimicrobial cyclodecapeptide, gramicidin S (GS). This study reports on some of the molecular aspects of the antagonism as investigated through complementary electrospray ionization mass spectrometry techniques. We were able to detect stable 1:1 and 2:1 hetero-oligomers in a mixture of surfactin and gramicidin S. The noncovalent interaction between GS and Srf, with the proposed equilibrium: GS~Srf↔GS+Srf correlated to apparent K d values of 6-9 μM in gas-phase and 1 μM in aqueous solution...
May 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28560563/mobility-peak-tailing-reduction-in-a-differential-mobility-analyzer-dma-coupled-with-a-mass-spectrometer-and-several-ionization-sources
#12
Mario Amo-Gonzalez, Juan Fernandez de la Mora
The differential mobility analyzer (DMA) is a narrow-band linear ion mobility filter operating at atmospheric pressure. It combines in series with a quadrupole mass spectrometer (Q-MS) for mobility/mass analysis, greatly reducing chemical noise in selected ion monitoring. However, the large flow rate of drift gas (~1000 L/min) required by DMAs complicates the achievement of high gas purity. Additionally, the symmetry of the drying counterflow gas at the interface of many commercial MS instruments, is degraded by the lateral motion of the drift gas at the DMA entrance slit...
May 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28560562/design-of-a-tw-slim-module-for-dual-polarity-confinement-transport-and-reactions
#13
Sandilya V B Garimella, Ian K Webb, Aneesh Prabhakaran, Isaac K Attah, Yehia M Ibrahim, Richard D Smith
Here we describe instrumental approaches for performing dual polarity ion confinement, transport, ion mobility separations, and reactions in structures for lossless ion manipulations (SLIM). Previous means of ion confinement in SLIM, based upon rf-generated pseudopotentials and DC fields for lateral confinement, cannot trap ions of opposite polarity simultaneously. Here we explore alternative approaches to provide simultaneous lateral confinement of both ion polarities. Traveling wave ion mobility (IM) separations experienced in such SLIM cause ions of both polarities to migrate in the same directions and exhibit similar separations...
May 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28560561/investigation-of-ion-transmission-effects-on-intact-protein-quantification-in-a-triple-quadrupole-mass-spectrometer
#14
Evelyn H Wang, Dananjaya Kalu Appulage, Erin A McAllister, Kevin A Schug
Recently, direct intact protein quantitation using triple quadrupole mass spectrometry (QqQ-MS) and multiple reaction monitoring (MRM) was demonstrated (J. Am. Soc. Mass Spectrom. 27, 886-896 (2016)). Even though QqQ-MS is known to provide extraordinary detection sensitivity for quantitative analysis, we found that intact proteins exhibited a less than 5% ion transmission from the first quadrupole to the third quadrupole mass analyzer in the presence of zero collision energy (ZCE). With the goal to enhance intact protein quantitation sensitivity, ion scattering effects, proton transfer effects, and mass filter resolution widths were examined for their contributions to the lost signal...
May 30, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28547725/dual-polarity-ion-trap-mass-spectrometry-dynamic-monitoring-and-controlling-gas-phase-ion-ion-reactions
#15
Muyi He, You Jiang, Dan Guo, Xingchuang Xiong, Xiang Fang, Wei Xu
A dual-polarity linear ion trap (LIT) mass spectrometer was developed in this study, and the method for simultaneously controlling and detecting cations and anions was proposed and realized in the LIT. With the application of an additional dipolar DC field on the ejection electrodes of an LIT, dual-polarity mass spectra could be obtained, which include both the mass-to-charge (m/z) ratio and charge polarity information of an ion. Compared with conventional method, the ion ejection and detection efficiency could also be improved by about one-fold...
May 25, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28534157/infrared-laser-ablation-with-vacuum-capture-for-fingermark-sampling
#16
Fabrizio Donnarumma, Eden E Camp, Fan Cao, Kermit K Murray
Infrared laser ablation coupled to vacuum capture was employed to collect material from fingermarks deposited on surfaces of different porosity and roughness. Laser ablation at 3 μm was performed in reflection mode with subsequent capture of the ejecta with a filter connected to vacuum. Ablation and capture of standards from fingermarks was demonstrated on glass, plastic, aluminum, and cardboard surfaces. Using matrix assisted laser desorption ionization (MALDI), it was possible to detect caffeine after spiking with amounts as low as 1 ng...
May 22, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28516297/subcritical-water-hydrolysis-of-peptides-amino-acid-side-chain-modifications
#17
Thomas Powell, Steve Bowra, Helen J Cooper
Previously we have shown that subcritical water may be used as an alternative to enzymatic digestion in the proteolysis of proteins for bottom-up proteomics. Subcritical water hydrolysis of proteins was shown to result in protein sequence coverages greater than or equal to that obtained following digestion with trypsin; however, the percentage of peptide spectral matches for the samples treated with trypsin were consistently greater than for those treated with subcritical water. This observation suggests that in addition to cleavage of the peptide bond, subcritical water treatment results in other hydrolysis products, possibly due to modifications of amino acid side chains...
May 17, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28516296/substrate-coated-illumination-droplet-spray-ionization-real-time-monitoring-of-photocatalytic-reactions
#18
Hong Zhang, Na Li, Dandan Zhao, Jie Jiang, Hong You
Real-time monitoring of photocatalytic reactions facilitates the elucidation of the mechanisms of the reactions. However, suitable tools for real-time monitoring are lacking. Herein, a novel method based on droplet spray ionization named substrate-coated illumination droplet spray ionization (SCI-DSI) for direct analysis of photocatalytic reaction solution is reported. SCI-DSI addresses many of the analytical limitations of electrospray ionization (ESI) for analysis of photocatalytic-reaction intermediates, and has potential for both in situ analysis and real-time monitoring of photocatalytic reactions...
May 17, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28510796/focus-on-bio-ion-chemistry-interactions-of-biological-ions-with-ions-molecules-surfaces-electrons-and-light-honoring-scott-a-mcluckey-recipient-of-the-2016-asms-award-for-a-distinguished-contribution-in-mass-spectrometry
#19
EDITORIAL
https://www.readbyqxmd.com/read/28508286/sample-preparation-of-corn-seed-tissue-to-prevent-analyte-relocations-for-mass-spectrometry-imaging
#20
Shin Hye Kim, Jeongkwon Kim, Young Jin Lee, Tae Geol Lee, Sohee Yoon
Corn seed tissue sections were prepared by the tape support method using an adhesive tape, and mass spectrometry imaging (MSI) was performed. The effect of heat generated during sample preparation was investigated by time-of-flight secondary mass spectrometry (TOF-SIMS) imaging of corn seed tissue prepared by the tape support and the thaw-mounted methods. Unlike thaw-mounted sample preparation, the tape support method does not cause imaging distortion because of the absence of heat, which can cause migration of the analytes on the sample...
May 15, 2017: Journal of the American Society for Mass Spectrometry
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