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Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology

Marta Viana-Pereira, Gisele Caravina Almeida, João Norberto Stavale, Susana Malheiro, Carlos Clara, Patrícia Lobo, José Pimentel, Rui Manuel Reis
Hotspot activating mutations of the telomerase reverse transcriptase (hTERT) promoter region were recently described in several tumor types. These mutations lead to enhanced expression of telomerase, being responsible for telomere maintenance and allowing continuous cell division. Additionally, there are alternative telomere maintenance mechanisms, associated with histone H3 mutations, responsible for disrupting the histone code and affecting the regulation of transcription. Here, we investigated the clinical relevance of these mechanistically related molecules in medulloblastoma...
October 1, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Theresa D Ahrens, Lisa Lutz, Silke Lassmann, Martin Werner
Barrett's esophagus (BE) is defined as metaplasia of the esophageal squamous epithelium with multiple cell layers into a single layer of intestinal columnar epithelial cells - or, in other words, skyscrapers are turned into town houses. The underlying pathomechanism(s) and the cell of origin of BE lesions have not been defined yet. However, four potential hypotheses for BE development have been suggested. The morphological changes during BE development are associated with rather well-described aberrant gene/protein expression patterns...
September 23, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Hirofumi Bekki, Kenichi Kohashi, Yuichi Yamada, Kunio Iura, Takeaki Ishii, Akira Maekawa, Hiroshi Otsuka, Hidetaka Yamamoto, Michiyuki Hakozaki, Kazuki Nabeshima, Yukihide Iwamoto, Yoshinao Oda
OBJECTIVE: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). METHODS: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples...
September 22, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Mariagrazia Granata, Evangelia Skarmoutsou, Chiara Trovato, Giulio A Rossi, Maria Clorinda Mazzarino, Fabio D'Amico
Obesity, type 1 diabetes, and psoriasis are wide-ranging health problems. Genetics, epigenetics, and environmental factors together with immune disturbances are involved in these diseases. The white adipose tissue is an active endocrine organ, secreting a wide variety of soluble mediators called adipokines that have a central role in the relationship between adipose tissue and immune system. Inflammatory cytokines, including the IL-23/IL-17 and IL-18 axes, and microRNAs are involved in many processes, including immunity and inflammation, thus having a major role in the onset of these three diseases...
September 17, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Alireza Akbarzadeh Baghban, Nasim Taghavi, Maryam Shahla
BACKGROUND: Overexpression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) and increasing mast cell density (MCD) in premalignant and malignant oral lesions have been documented. However, their correlation with clinicopathologic parameters and survival rate in oral squamous cell carcinoma (OSCC) is not completely clear. This study aimed to assess these subjects. METHODS: VEGF, COX-2, and mast cell tryptase expression were examined immunohistochemically in 57 cases of OSCC...
September 1, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Vega Karlowee, Vishwa Jeet Amatya, Hirofumi Hirano, Takeshi Takayasu, Ryo Nosaka, Manish Kolakshyapati, Masako Yoshihiro, Yukio Takeshima, Kazuhiko Sugiyama, Kazunori Arita, Kaoru Kurisu, Fumiyuki Yamasaki
Multicentric gliomas are very rare. Due to differences in their tumor types they remain enigmatic. We focused on the pathogenesis of multicentric gliomas and compared their immunoprofile with that of solitary gliomas. This retrospective study included 6 males and 8 females with multicentric glioma (8 glioblastomas, 2 anaplastic astrocytomas, 4 diffuse astrocytomas). Their age ranged from 27 to 75 years and all were treated between 2004 and June 2015. The expression of mutant isocitrate dehydrogenase 1 (IDH1), α-thalassemia X-linked intellectual disability (ATRX), p53, phosphatase and tensin homolog (PTEN), and epidermal growth factor receptor (EGFR) was examined immunohistochemically; for 1p19q analysis we used fluorescence in situ hybridization (FISH)...
August 24, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Hye-Young Wang, Sungwoo Ahn, Sunyoung Park, SeungIl Kim, Hyeyoung Lee
BACKGROUND: Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods. METHODS: In this study, we analyzed HER2 mRNA expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using a one-tube nested reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay...
August 20, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Thomas Menter, Matthias Bachmann, Susanne Grieshaber, Alexandar Tzankov
Amyloidosis is the result of various, differently approachable diseases. It is vital to subtype the amyloid deposits in order to establish and finally treat the underlying disease properly. Besides the classical staining with Congo red, further procedures like immunohistochemical staining are needed for classification. Here, we present a more accurate approach using Congo red/immunohistochemical double staining easily applicable in routine diagnostics. Modifications of the Congo red staining technique and the immunohistochemical procedures were needed in order to combine both staining procedures on one slide...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Takeharu Imai, Naohide Oue, Masahiro Nishioka, Shoichiro Mukai, Takashi Oshima, Naoya Sakamoto, Kazuhiro Sentani, Keisuke Matsusaki, Kazuhiro Yoshida, Wataru Yasui
OBJECTIVE: Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is >2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry. METHODS: Expression of KIF11 in 165 GC cases was determined using immunohistochemistry...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Li-Min Long, Jun-Kun Zhan, Hai-Qin Wang, Shuang Li, Yi-Yin Chen, You-Shuo Liu
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) exhibits poor prognosis and resistance to chemotherapy. This study was to identify the biomarkers associated with the progression, poor prognosis and chemoresistance of PDAC. METHODS: miR-34a and miR-150 levels in the plasma and tissues from PDAC patients were measured by real-time PCR. Xenograft PDAC tumor models were established in mice by inoculation of CD133+ stem cells isolated from PDAC tumors. Protein expression was measured by Western blot...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Yasuhiro Aso, Kenichiro Nakamura, Noriyuki Kimura, Makoto Takemaru, Ryuki Arakawa, Minoru Fujiki, Teruyuki Hirano, Toshihide Kumamoto, Etsuro Matsubara
BACKGROUND: Cerebrovascular white matter lesions (WMLs) are associated with cognitive impairment in patients with subcortical vascular dementia. We performed a comprehensive gene expression analysis to elucidate genes associated with WML development in a chronic cerebral hypoperfusion rat model. METHODS: Brains of rats with bilateral carotid ligation (2VO, n = 10) and sham-operated rats (n = 5-10/group) were removed on days 1, 7, or 28 after surgery. Total RNA isolated from the corpus callosum was evaluated by microarray analysis and quantitative reverse transcription-polymerase chain reaction...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Liesbeth Allais, Rebecca De Smet, Stephanie Verschuere, Karel Talavera, Claude A Cuvelier, Tania Maes
Inflammatory bowel disease (IBD) is characterized by severe gastrointestinal inflammation and results from a complex interplay between genetic and environmental factors. IBD includes two prominent subtypes: Crohn's disease (CD) and ulcerative colitis (UC). One of the main risk factors for the development of CD is cigarette smoking, while UC is rather a disease of ex-smokers. To date, many of the mechanisms underlying the immune imbalance in IBD and the involvement of cigarette smoke (CS) are incompletely understood...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Astrid De Meulenaere, Tijl Vermassen, Sandrine Aspeslagh, Karen Zwaenepoel, Philippe Deron, Fréderic Duprez, Liesbeth Ferdinande, Sylvie Rottey
OBJECTIVE: Over the last decade, efforts have been made to get a better understanding of the tumor microenvironment and the role of the immune system in it. New insights into the CD27/CD70 signaling pathway point towards a role in tumor immunology, making CD70 an attractive target for immunotherapy. Here, we evaluate CD70 expression in squamous cell carcinoma of the head and neck (SCCHN). METHODS: CD70 immunohistochemistry was retrospectively performed on 95 tumor samples...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Maíra Assis Lima, Liliane Dos Santos, José Antonio Turri, Suely Nonogaki, Marcilei Buim, Joema Felipe Lima, João de Jesus Viana Pinheiro, Cynthia Aparecida Bueno de Toledo Osório, Fernando Augusto Soares, Vanessa Morais Freitas
BACKGROUND: ADAMTS are metalloproteases with disintegrin and thrombospondin motifs. They are secreted proteases playing a role in biological processes such as inflammation, angiogenesis, and urogenital development. ADAMTS have specific substrates, such as the proteoglycans (PG) versican, aggrecan, and brevican. Despite data indicating a role of ADAMTS in tumor invasion and metastases, effects played by these molecules in cancer progression are still controversial. In ovarian cancer, the importance of ADAMTS gene mutations was recently described and related to chemotherapy outcome...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Hiroaki Niitsu, Takao Hinoi, Kazuhiro Sentani, Shoichiro Mukai, Tomohiro Adachi, Yasufumi Saito, Masashi Miguchi, Masatoshi Kochi, Haruki Sada, Naohide Oue, Wataru Yasui, Hideki Ohdan
OBJECTIVE: Increased expression of calcineurin in colorectal cancer (CRC) has been reported. Although the oncogenic function has been suggested, the clinical relevance is still unclear. We herein studied calcineurin expression as a prognostic biomarker in patients receiving curative surgery for stages I-III CRC. METHODS: In 121 patients with stages I-III CRC treated at Hiroshima University between 1997 and 2003, calcineurin A expression was examined using immunohistochemistry (IHC) staining of surgical specimens...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Manuel Schlageter, Luca Quagliata, Matthias Matter, Valeria Perrina, Luigi Tornillo, Luigi Terracciano
OBJECTIVES: Analysis of a large local autopsy collective to gather epidemiological and histopathological data on hepatocellular carcinoma (HCC). METHODS: We examined a large dataset of 44,104 autopsies performed at the Institute of Pathology, Basel, Switzerland, including 2 autopsy collectives (1969-1983 and 1988-2012) to gather current data on HCC in the advanced stage. A total of 398 HCC were diagnosed, accounting for around 1% of all autopsies. RESULTS: As expected, most patients developing HCC had advanced stages of liver fibrosis or cirrhosis (F3/F4)...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Manal Wilson, Amany A Abou-Elalla, Mervat Talaat Zakaria, Huda Marzouk, Hala Lotfy Fayed, Mariam Onsy F Hanna
BACKGROUND: Since spontaneous inflammation is an important contributor to familial Mediterranean fever (FMF), genetic variants mediating inflammation are of interest. We investigated gene variants in the acute-phase serum amyloid A type 1 (SAA1), a sensitive marker of inflammatory activity, and their association with susceptibility and severity of FMF. METHODS: The genotypes of 2 single-nucleotide polymorphisms within exon 3 of SAA1 (2995C/T and 3010C/T) were determined in 105 Egyptian children with FMF and in 125 controls by polymerase chain reaction-restriction fragment length polymorphism...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Yuko Kusakabe, Yumiko Shindo, Takayuki Kawai, Yoko Takahashi, Masuko Kobori, Hiroko Inoue, Ichiro Saito
OBJECTIVE: The decrease in female hormone levels at menopause affects whole-body homeostasis. Various therapies including hormone therapy and treatment with herbal supplements are available to improve menopausal symptoms. However, a method for evaluating their effectiveness has not been established. We sought to identify useful biomarkers to assess therapy efficacy. METHODS: We searched for salivary proteins affected by changes in female hormone levels in mouse submandibular glands...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
Masaaki Sugimoto, Kenichi Kohashi, Momoe Itsumi, Masaki Shiota, Tatsuro Abe, Yuichi Yamada, Kentaro Kuroiwa, Seiji Naito, Yoshinao Oda
AIMS: The aims of this study were to investigate the association of renal cell carcinoma (RCC) displaying rhabdoid features and morphologically mesenchymal characteristics with epithelial to mesenchymal transition (EMT), and to clarify the expression of EMT markers. METHODS: We investigated the expression of EMT markers (E-cadherin, vimentin, Snail, Slug, ZEB1, ZEB2 and Twist1) using immunohistochemistry, Western blotting and real-time polymerase chain reaction in 18 cases of clear cell RCC (ccRCC) with rhabdoid features and 74 ccRCC cases with Fuhrman grade 1-3 (G1 to G3)...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
William Sterlacci, Michael Fiegl, Raoul A Droeser, Alexandar Tzankov
OBJECTIVES: In light of various trials showing impressive response rates when treating non-small cell lung cancer (NSCLC) patients with anti PD-1/PD-L1 antibodies, the currently equivocal role of PD-L1 expression in NSCLC is in need of further clarification. METHODS: We therefore analyzed the expression of PD-L1 on 293 well-documented NSCLC cases and correlated the results with clinical, histopathological and immunohistochemical characteristics. RESULTS: The expression of PD-L1 on NSCLC was a poor prognostic factor for patients with nodal-negative adenocarcinoma (ACA) and, independent of other covariates, in tumors with increased CD8+ tumor-infiltrating lymphocytes (TILs)...
2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
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