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Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology

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https://www.readbyqxmd.com/read/28434003/cytotoxic-effects-of-transthyretin-aggregates-in-an-epidermoid-cell-line
#1
Vai Hong Fong, Amandio Vieira
OBJECTIVE: Skin amyloid deposits can occur as part of systemic amyloidoses including those involving misfolded- aggregated transthyretins (agTTR). Pathological effects of agTTR on the skin are not well understood. The main objective of the current study was to examine the toxicity of agTTR upon a human keratinocyte cell line. METHODS: Cells were analyzed for indicators of oxidative stress after treatment with normal soluble TTR or the same pre-aggregation concentration of agTTR...
April 22, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28399526/expression-of-the-erbb-family-of-ligands-and-receptors-in-gastric-cancer
#2
Sun-Ju Byeon, Hye Seung Lee, Min-A Kim, Byung Lan Lee, Woo Ho Kim
OBJECTIVE: Gastric cancer (GC) is the second most common cancer and the third leading cause of cancer-related death in Korea. Alterations in the ERBB (homology to the erythroblastoma viral gene product, v-erbB) receptor family and ERBB-related signaling pathways are frequently observed in GC. However, the roles of the ERBB receptors and their ligands in GC are not well established. METHODS: We evaluated the expression levels of various ERBB receptor ligands (i.e...
April 12, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28291966/molecular-characteristics-of-pancreatic-ductal-adenocarcinomas-with-high-grade-pancreatic-intraepithelial-neoplasia-panin-are-different-from-those-without-high-grade-panin
#3
Tetsuyuki Miyazaki, Yoshihiro Ohishi, Yoshihiro Miyasaka, Yasunori Oda, Shinichi Aishima, Keigo Ozono, Atsushi Abe, Eishi Nagai, Masafumi Nakamura, Yoshinao Oda
AIMS: We reported that pancreatic ductal adenocarcinomas (PDACs) without high-grade pancreatic intraepithelial neoplasia (PanIN) in the vicinity had worse prognoses than PDACs with high-grade PanIN. However, the molecular characteristics of PDACs with and without high-grade PanIN have not been compared. The aim of this study is to clarify the molecular characteristics of PDACs with and without high-grade PanIN. METHOD AND RESULTS: We reviewed all of a consecutive series of 100 patients with PDACs and divided them into 2 groups: the PDACs with PanIN-2 or PanIN-3 in the background (the PanIN-high group, n = 60) and the PDACs without PanIN-2 or PanIN-3 in the background (the PanIN-low group, n = 40)...
March 15, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28288473/expression-pattern-of-smad4-gata3-as-a-predictor-of-survival-in-invasive-ductal-carcinoma-of-the-breast
#4
Kyueng-Whan Min, Dong-Hoon Kim, Sung-Im Do, Seoung Wan Chae, Kyungeun Kim, Jin Hee Sohn, Hyun Joo Lee, In-Gu Do, Jung-Soo Pyo, Yuil Kim, Dong Hyun Kim, Jung-Ho Yang, Sang-Jo Lee, Young Ha Oh, Sukjoong Oh, Seon Hyeong Choi, Yong Lai Park, Chan Heun Park, Eun-Kyung Kim, Mi Jung Kwon, Jinwon Seo
BACKGROUND: Smad4 and GATA3 proteins are known prognostic markers in various cancers. Smad4 is a mediator linked to both tumour suppression and progression. GATA3 is a regulator of development and morphogenesis of the mammary gland. We assessed and compared the predictive performance of Smad4 and GATA3 for clinical outcomes in patients with breast cancer. METHODS: The combined expression pattern based on Smad4+/- and GATA3+/- was evaluated by immunostaining using breast cancer tissue microarray, and the relationships between protein expression and clinicopathological variables were analysed...
March 14, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28178700/genetic-profiling-of-cancers-of-the-digestive-system-biological-insights-and-clinical-implications
#5
Albrecht Stenzinger, Wilko Weichert
Massive parallel sequencing technologies (next-generation sequencing) have enabled us to draw a comprehensive landscape of the genomic aberrations underlying common cancers of the digestive system, and they have thus revolutionized our understanding of the genomic makeup and biology of these tumors. Apart from the commonly mutated founder genes, e.g., KRAS and TP53, we now have detailed information on additional and less frequent genomic events for every major digestive system cancer. However, many challenging issues remain when it comes to translating these findings into clinical applications...
February 9, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28095381/a-single-oral-dose-of-geranylgeranylacetone-upregulates-vascular-endothelial-growth-factor-and-protects-against-kainic-acid-induced-neuronal-cell-death-involvement-of-the-phosphatidylinositol-3-kinase-akt-pathway
#6
Yukari Kawasaki, Minoru Fujiki, Susumu Uchida, Masaki Morishige, Yasutomo Momii, Keisuke Ishii
BACKGROUND: Previous studies demonstrated the cytoprotective effect of geranylgeranylacetone (GGA), a heat shock protein inducer, against ischemic insult or kainic acid (KA)-induced neuronal cell death. Phosphatidylinositol-3 kinase (PI3K)/Akt is thought to be an important factor that mediates neuroprotection. However, the signaling pathways in the brain in vivo after oral GGA administration remain unclear. METHODS: We measured and compared hippocampal neuron density to investigate the effect of GGA on KA-induced cell death in rats...
January 18, 2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27694758/study-of-htert-and-histone-3-mutations-in-medulloblastoma
#7
Marta Viana-Pereira, Gisele Caravina Almeida, João Norberto Stavale, Susana Malheiro, Carlos Clara, Patrícia Lobo, José Pimentel, Rui Manuel Reis
Hotspot activating mutations of the telomerase reverse transcriptase (hTERT) promoter region were recently described in several tumor types. These mutations lead to enhanced expression of telomerase, being responsible for telomere maintenance and allowing continuous cell division. Additionally, there are alternative telomere maintenance mechanisms, associated with histone H3 mutations, responsible for disrupting the histone code and affecting the regulation of transcription. Here, we investigated the clinical relevance of these mechanistically related molecules in medulloblastoma...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27656900/turning-skyscrapers-into-town-houses-insights-into-barrett-s-esophagus
#8
REVIEW
Theresa D Ahrens, Lisa Lutz, Silke Lassmann, Martin Werner
Barrett's esophagus (BE) is defined as metaplasia of the esophageal squamous epithelium with multiple cell layers into a single layer of intestinal columnar epithelial cells - or, in other words, skyscrapers are turned into town houses. The underlying pathomechanism(s) and the cell of origin of BE lesions have not been defined yet. However, four potential hypotheses for BE development have been suggested. The morphological changes during BE development are associated with rather well-described aberrant gene/protein expression patterns...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27639922/obesity-type-1-diabetes-and-psoriasis-an-autoimmune-triple-flip
#9
REVIEW
Mariagrazia Granata, Evangelia Skarmoutsou, Chiara Trovato, Giulio A Rossi, Maria Clorinda Mazzarino, Fabio D'Amico
Obesity, type 1 diabetes, and psoriasis are wide-ranging health problems. Genetics, epigenetics, and environmental factors together with immune disturbances are involved in these diseases. The white adipose tissue is an active endocrine organ, secreting a wide variety of soluble mediators called adipokines that have a central role in the relationship between adipose tissue and immune system. Inflammatory cytokines, including the IL-23/IL-17 and IL-18 axes, and microRNAs are involved in many processes, including immunity and inflammation, thus having a major role in the onset of these three diseases...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27577989/combined-analysis-of-vascular-endothelial-growth-factor-expression-with-cyclooxygenase-2-and-mast-cell-density-in-oral-squamous-cell-carcinoma
#10
Alireza Akbarzadeh Baghban, Nasim Taghavi, Maryam Shahla
BACKGROUND: Overexpression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) and increasing mast cell density (MCD) in premalignant and malignant oral lesions have been documented. However, their correlation with clinicopathologic parameters and survival rate in oral squamous cell carcinoma (OSCC) is not completely clear. This study aimed to assess these subjects. METHODS: VEGF, COX-2, and mast cell tryptase expression were examined immunohistochemically in 57 cases of OSCC...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27553586/multicentric-glioma-develops-via-a-mutant-idh1-independent-pathway-immunohistochemical-study-of-multicentric-glioma
#11
Vega Karlowee, Vishwa Jeet Amatya, Hirofumi Hirano, Takeshi Takayasu, Ryo Nosaka, Manish Kolakshyapati, Masako Yoshihiro, Yukio Takeshima, Kazuhiko Sugiyama, Kazunori Arita, Kaoru Kurisu, Fumiyuki Yamasaki
Multicentric gliomas are very rare. Due to differences in their tumor types they remain enigmatic. We focused on the pathogenesis of multicentric gliomas and compared their immunoprofile with that of solitary gliomas. This retrospective study included 6 males and 8 females with multicentric glioma (8 glioblastomas, 2 anaplastic astrocytomas, 4 diffuse astrocytomas). Their age ranged from 27 to 75 years and all were treated between 2004 and June 2015. The expression of mutant isocitrate dehydrogenase 1 (IDH1), α-thalassemia X-linked intellectual disability (ATRX), p53, phosphatase and tensin homolog (PTEN), and epidermal growth factor receptor (EGFR) was examined immunohistochemically; for 1p19q analysis we used fluorescence in situ hybridization (FISH)...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27544315/clinical-usefulness-of-a-one-tube-nested-reverse-transcription-quantitative-polymerase-chain-reaction-assay-for-evaluating-human-epidermal-growth-factor-receptor-2-mrna-overexpression-in-formalin-fixed-and-paraffin-embedded-breast-cancer-tissue-samples
#12
Hye-Young Wang, Sungwoo Ahn, Sunyoung Park, SeungIl Kim, Hyeyoung Lee
BACKGROUND: Currently, the two main methods used to analyze human epidermal growth factor receptor 2 (HER2) amplification or overexpression have a limited accuracy and high costs. These limitations can be overcome by the development of complementary quantitative methods. METHODS: In this study, we analyzed HER2 mRNA expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using a one-tube nested reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27487341/a-more-accurate-approach-to-amyloid-detection-and-subtyping-combining-in-situ-congo-red-staining-and-immunohistochemistry
#13
Thomas Menter, Matthias Bachmann, Susanne Grieshaber, Alexandar Tzankov
Amyloidosis is the result of various, differently approachable diseases. It is vital to subtype the amyloid deposits in order to establish and finally treat the underlying disease properly. Besides the classical staining with Congo red, further procedures like immunohistochemical staining are needed for classification. Here, we present a more accurate approach using Congo red/immunohistochemical double staining easily applicable in routine diagnostics. Modifications of the Congo red staining technique and the immunohistochemical procedures were needed in order to combine both staining procedures on one slide...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27459100/overexpression-of-kif11-in-gastric-cancer-with-intestinal-mucin-phenotype
#14
Takeharu Imai, Naohide Oue, Masahiro Nishioka, Shoichiro Mukai, Takashi Oshima, Naoya Sakamoto, Kazuhiro Sentani, Keisuke Matsusaki, Kazuhiro Yoshida, Wataru Yasui
OBJECTIVE: Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is >2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry. METHODS: Expression of KIF11 in 165 GC cases was determined using immunohistochemistry...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27458977/the-clinical-significance-of-mir-34a-in-pancreatic-ductal-carcinoma-and-associated-molecular-and-cellular-mechanisms
#15
Li-Min Long, Jun-Kun Zhan, Hai-Qin Wang, Shuang Li, Yi-Yin Chen, You-Shuo Liu
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) exhibits poor prognosis and resistance to chemotherapy. This study was to identify the biomarkers associated with the progression, poor prognosis and chemoresistance of PDAC. METHODS: miR-34a and miR-150 levels in the plasma and tissues from PDAC patients were measured by real-time PCR. Xenograft PDAC tumor models were established in mice by inoculation of CD133+ stem cells isolated from PDAC tumors. Protein expression was measured by Western blot...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27458816/induction-of-genes-expressed-in-endothelial-cells-of-the-corpus-callosum-in-the-chronic-cerebral-hypoperfusion-rat-model
#16
Yasuhiro Aso, Kenichiro Nakamura, Noriyuki Kimura, Makoto Takemaru, Ryuki Arakawa, Minoru Fujiki, Teruyuki Hirano, Toshihide Kumamoto, Etsuro Matsubara
BACKGROUND: Cerebrovascular white matter lesions (WMLs) are associated with cognitive impairment in patients with subcortical vascular dementia. We performed a comprehensive gene expression analysis to elucidate genes associated with WML development in a chronic cerebral hypoperfusion rat model. METHODS: Brains of rats with bilateral carotid ligation (2VO, n = 10) and sham-operated rats (n = 5-10/group) were removed on days 1, 7, or 28 after surgery. Total RNA isolated from the corpus callosum was evaluated by microarray analysis and quantitative reverse transcription-polymerase chain reaction...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27388890/transient-receptor-potential-channels-in-intestinal-inflammation-what-is-the-impact-of-cigarette-smoking
#17
REVIEW
Liesbeth Allais, Rebecca De Smet, Stephanie Verschuere, Karel Talavera, Claude A Cuvelier, Tania Maes
Inflammatory bowel disease (IBD) is characterized by severe gastrointestinal inflammation and results from a complex interplay between genetic and environmental factors. IBD includes two prominent subtypes: Crohn's disease (CD) and ulcerative colitis (UC). One of the main risk factors for the development of CD is cigarette smoking, while UC is rather a disease of ex-smokers. To date, many of the mechanisms underlying the immune imbalance in IBD and the involvement of cigarette smoke (CS) are incompletely understood...
2017: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28002815/cortical-actin-alteration-at-the-matrix-side-cytoplasm-in-lung-adenocarcinoma-cells-and-its-significance-in-invasion
#18
Shohei Tomii, Takumi Akashi, Noboru Ando, Tomoki Tamura, Akira Sakurai, Asami Terada, Asuka Furukawa, Yoshimi Suzuki, Kou Kayamori, Kei Sakamoto, Hironori Ishibashi, Yoshinobu Eishi
OBJECTIVES: Cortical actin is a thin layer of filamentous (F-)actin that lies beneath the plasma membrane, and its role in pathophysiology remains unclear. We investigated the subcellular localization of cortical actin by the histopathological and experimental studies of lung adenocarcinomas. MATERIALS AND METHODS: The subcellular localization of cortical actin was studied in surgically resected lung adenocarcinomas tissues and in 3-dimensionally cultured lung adenocarcinoma A549 cells...
December 22, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27974721/liquid-biopsy-a-future-tool-for-posttreatment-surveillance-in-head-and-neck-cancer
#19
Joost H van Ginkel, Manon M H Huibers, Rob Noorlag, Remco de Bree, Robert J J van Es, Stefan M Willems
The prognosis of head and neck squamous cell carcinoma (HNSCC) is largely based on disease stage. Despite improvements in treatment, recurrence rates are still considered high. Currently, disease progression or regression after curative treatment is monitored by clinical evaluation combined with flexible endoscopy and/or imaging. However, specificity of imaging is low due to the posttreatment effects. Detection of circulating tumor DNA (ctDNA) from blood samples of HNSCC patients is a minimally invasive technique that could lead to an earlier detection of recurrence...
December 15, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27923229/hands-on-experience-accreditation-of-pathology-laboratories-according-to-iso-15189
#20
Alexandar Tzankov, Luigi Tornillo
Accreditation is a procedure by which an authoritative body gives formal recognition that an organization is competent to carry out specific tasks according to certain standards. Accreditation of pathology laboratories according to ISO 15189 is now becoming more and more a matter of course in continental Europe. This review describes some practical experience aspects with our own pathology laboratory accreditation according to ISO 15189, and outlines the advantages, addresses critical points, and discusses certain caveats of this process...
December 7, 2016: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
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