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DNA and Cell Biology

Hai-Bo Li, Ruo-Xi Wang, Hai-Bo Jiang, En-Dong Zhang, Jie-Qiong Tan, Hui-Zhuo Xu, Rong-Rong Zhou, Xiao-Bo Xia
Mitochondrial ribosomal proteins are important for mitochondrial-encoded protein synthesis and mitochondrial function. In addition to their roles in mitoribosome assembly, several mitochondrial ribosome proteins are also implicated in cellular processes like cell cycle regulation, apoptosis, and mitochondrial homeostasis regulation. Here, we demonstrate that MRPL10 regulates cyclin B1/Cdk1 (cyclin-dependent kinase 1) activity and mitochondrial protein synthesis in mammalian cells. In Drosophila, inactivation of mRpL10 (the Drosophila ortholog of mammalian MRPL10) in eyes results in abnormal eye development...
October 11, 2016: DNA and Cell Biology
Guojian Sun, Bin Luan, Ruiying Zhao, Zhanbiao Li, Zhangmin Xing
The purpose of this study was to introduce a new method to elucidating the molecular mechanisms in ischemic stroke. Genes from microarray data were performed enrichment to biological pathways. Dysregulated pathways and dysregulated pathway pairs were identified and constructed into networks. After Random Forest classification was performed, area under the curve (AUC) value of main network was calculated. After 50 bootstraps of Monte Carlo Cross-Validation, six pairs of pathways were found for >40 times. The best main network with AUC value = 0...
October 11, 2016: DNA and Cell Biology
Ji Chen, Feng Deng, Mengsheng Deng, Jincheng Han, Jianbin Chen, Li Wang, Shen Yan, Kai Tong, Fan Liu, Mengliang Tian
Bacterial homologous chloroplast-targeted Obg GTPases (ObgCs) belong to the plant-typical Obg group, which is involved in diverse physiological processes during chloroplast development. However, the evolutionarily conserved function of ObgC in plants remains elusive and requires further investigation. In this study, we identified DoObgC from an epiphytic plant Dendrobium officinale and demonstrated the characteristics of DoObgC. Sequence analysis indicated that DoObgC is highly conserved with other plant ObgCs, which contain the chloroplast transit peptide (cTP), Obg fold, G domain, and OCT regions...
October 6, 2016: DNA and Cell Biology
Yang Song, Zhenwu Du, Qiwei Yang, Ming Ren, Yujie Sui, Qingyu Wang, Ao Wang, Haiyue Zhao, Jincheng Wang, Guizhen Zhang
The critical roles of IGFBP3 in regulating the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells strongly indicate its potential effects on the pathogenesis of osteonecrosis of the femoral head (ONFH). In this study, we investigated the association of IGFBP3 gene polymorphism and its protein expression with the development of ONFH to further explore its molecular pathogenesis. Ligase detection reactions and enzyme-linked immunosorbent assay were used to detect the polymorphisms of rs2453839[C/T] and rs3110697[A/G] and the serum protein expression of IGFBP3 gene in 182 cases and 179 controls, respectively...
October 5, 2016: DNA and Cell Biology
Guozhen Liu, Jing Luan, Qiang Li
Diffuse large B cell lymphoma (DLBCL) is a common B cell malignancy. Complete remission can be achieved in most patients by conventional treatment with rituximab and chemotherapy. However, a subset of remission individuals will develop a relapsed disease for obscure reasons. CD4(+)Foxp3(-)IL-10(+) cell (Tr1) is a novel cell subtype with the capacity to suppress pro-inflammatory responses, but has not been extensively studied in most tumors. In this study, we investigated the potential role of Tr1 cells in DLBCL...
October 5, 2016: DNA and Cell Biology
Xiangjie Kong, Xiaoqiang Qian, Liujian Duan, Hailong Liu, Yingjian Zhu, Jun Qi
Prostate cancer (PCa) is one of the most prevalent malignant tumors. microRNAs (miRNAs) play an important role in cancer initiation, progression, and metastasis, and their roles in PCa are becoming more apparent. In this study, we found that microRNA-372 (miR-372) is downregulated in human PCa and inhibits the proliferation activity, migration, and invasion of DU145 cells. Subsequently, p65 is confirmed as a target of miR-372, and knockdown of p65 expression similarly resulted in decreased proliferation activity, migration, and invasion...
September 27, 2016: DNA and Cell Biology
TianJun Chen, Hui Ren, Asmitanand Thakur, Tian Yang, Yang Li, Shuo Zhang, Ting Wang, MingWei Chen
Klotho is originally discovered as an anti-aging gene and recently identified as a tumor suppressor in various human cancers. Drug resistance is a major obstacle to affect the treatment of chemotherapy. In the present study, we explore the role of klotho on drug resistance in human lung cancers and investigate the mechanism of klotho on drug resistance in lung cancer cells. First, we detected a panel of six human lung cancer cell lines, including H460, SK-MES-1, cisplatin (DDP)-resistant A549/DDP, its parental subline A549, docetaxel (DTX)-resistant SPC-A-1/DTX, and SPC-A-1 by western blotting analysis...
September 23, 2016: DNA and Cell Biology
Zhaoe Liu, Bo Han, Xing Chen, Qiaoling Wu, Lijun Wang, Gang Li
Pycnogenol(®) (PYC) is utilized in the treatment of various diseases ranging from chronic inflammation to circulatory diseases, but its efficacy and functional mechanism in pediatric asthma continue to remain obscure. Therefore, the purpose of this study was to investigate the effectiveness and molecular mechanism of PYC on regulation of asthmatic airway inflammation. We found that PYC with tail intravenous injection of 50 mg/kg or intragastric administration of 100 mg/kg all reduced ovalbumin (OVA)-induced airway injury...
September 19, 2016: DNA and Cell Biology
Aaron R Massey, J David Beckham
Alpha-synuclein (α-syn) is a highly conserved protein encoded by the SNCA gene and is expressed uniquely in neurons of both the central and peripheral nervous systems (CNS and PNS). α-Syn is known to cause sporadic and familial forms of Parkinson's disease (PD). However, the role for neuronal expression of α-syn in the first place remains unknown. We review and discuss recently published work that suggests a novel role for α-syn expression in neurons as a restriction factor that inhibits virus transmission from the PNS to the CNS...
September 15, 2016: DNA and Cell Biology
Chuan Huang, Yan-Wei Hu, Jing-Jing Zhao, Xin Ma, Yuan Zhang, Feng-Xia Guo, Chun-Min Kang, Jing-Bo Lu, Jian-Cheng Xiu, Yan-Hua Sha, Ji-Juan Gao, Yan-Chao Wang, Pan Li, Bang-Ming Xu, Lei Zheng, Qian Wang
Atherosclerosis is a common pathological basis of cardiovascular disease, which remains the leading cause of mortality. Long non-coding RNAs (lncRNAs) are newly studied non-protein-coding RNAs involved in gene regulation, but how lncRNAs exert regulatory effect on atherosclerosis remains unclear. In this study, we found that lncRNA HOXC cluster antisense RNA 1 (HOXC-AS1) and homeobox C6 (HOXC6) were downregulated in carotid atherosclerosis by performing microarray analysis. The results were verified in atherosclerotic plaques and normal arterial intima tissues by quantitative reverse transcription PCR and western blot analysis...
August 30, 2016: DNA and Cell Biology
Matías González-Quiroz, Ximena Calderón, Ingrid Oyarzún, Claudia Hoepfner, Andrés Azócar, Adam Aguirre, Karin Álvarez, Rodrigo Quera, Francisco López-Köstner, Jaime Meléndez
High incidence of Rho Cdc42-GTPase overexpression has been found in Colorectal Cancer (CRC) samples, suggesting its potential role in tumor development. However, no conclusive studies have shown the lack of mutations and/or copy number of Cdc42 gene in this type of samples. To understand mutation/deletion and copy number status of Cdc42 gene, CRC patients were evaluated for both parameters. More than Cdc42 mutants, single-nucleotide variants were found. Analysis of regions flanking the Cdc42 gene showed allelic imbalance; 58...
August 19, 2016: DNA and Cell Biology
Gang Zhang, Yadi Wu, Dong Xu, Xinfeng Yan
Osteoarthritis (OA) is one of the most common prevalent chronic joint diseases. Emerging pieces of evidence have demonstrated that chondrocytes survival was closely associated with the destruction of joints in OA patients. Long noncoding RNAs (lncRNAs), defined as >200 nucleotides in length, also have been implicated in a variety of disease states. However, there are few studies on the role of lncRNAs in OA, and the pathological contributions of lncRNAs to OA remain largely unknown. In this study, we examined the expression of lncRNA UFC1 in cartilage samples from OA patients and healthy subjects, and then investigated biological function of UFC1 in OA chondrocyte...
August 16, 2016: DNA and Cell Biology
Huayu Sun, Lichao Li, Yongfeng Lou, Hansheng Zhao, Yihong Yang, Zhimin Gao
UDP-galactose-4-epimerase (UGE) is a key enzyme involved in galactose metabolism by catalyzing the interconversion of UDP-glucose to UDP-galactose. The cDNA encoding UGE was isolated from Phyllostachys edulis by reverse transcription-polymerase chain reaction and by 5' and 3' rapid amplification of cDNA ends, and was designated as PeUGE. The full-length cDNA of PeUGE was 1778 bp, which contained an open reading frame (ORF) encoding a peptide of 420 amino acids, with a calculated molecular mass of 46.58 kDa and a theoretic isoelectric point of 9...
August 15, 2016: DNA and Cell Biology
Xiaojian Zhao, Caiping Lu, Weiwei Chu, Yaxiao Zhang, Bing Zhang, Qiang Zeng, Renfeng Wang, Zhe Li, Baolei Lv, Jiabao Liu
Lung cancer is one of the most malignant cancers with a high metastatic potential. The purpose of this study was to study the role and the underlying mechanism of miR-214 in lung cancer progression. The expression of miR-214 in normal lung and lung cancer tissue was analyzed by quantitative real-time PCR analysis. Furthermore, H1299 cells were infected with miR-214 lentivirus, and the effect of infection on cell viability and migration was analyzed. Carboxypeptidase-D (CPD), as a potential target of miR-214, was characterized in either normal lung or lung cancer tissues...
August 5, 2016: DNA and Cell Biology
Arezoo Rajabian, Mohammad Taher Boroushaki, Parichehr Hayatdavoudi, Hamid Reza Sadeghnia
This study was designed to investigate whether the extract from Boswellia serrata oleo-gum resin (BSE) can protect against glutamate-induced oxidative damage and cytotoxicity in PC12 and N2a cell lines. Using a simple and reliable reverse-phase high-performance liquid chromatography (HPLC), the amount of 3-acetyl-11-keto-β-boswellic acid (AKBA) in the BSE was found to be 18.5% w/w. The results confirmed that BSE and AKBA, at concentrations as high as 100 μg/mL or 10 μM, respectively, caused no significant cytotoxicity or apoptotic cell death...
August 5, 2016: DNA and Cell Biology
Mengjiao Zhou, Chuanmin Li, Chunyan Lu, Xiaojun Zhang, Yunzhi Pan, Xin Liu, Gang Liu, Zhihui Zhao, Boxing Sun
miRNAs are involved in various biological processes, such as host-virus interactions and antiviral immunity. In this study, we investigated the role of miR-29 on porcine reproductive and respiratory syndrome virus (PRRSV) replication and its target genes. At first, miR-29a/b-1/c expression was detected when porcine alveolar macrophages (PAMs) were infected with PRRSV at different infective doses by real time-quantitative polymerase chain reaction (RT-qPCR). The result showed that miR-29a/b-1 expression significantly increased after 6 h (p < 0...
October 2016: DNA and Cell Biology
Shahram Teimourian, Ehsan Moghanloo
Abnormal cell differentiation, in particular suppression of terminal cell differentiation, exists in all tumors. Therapeutic interventions to restore terminal differentiation ("differentiation therapy") are a very attractive way to treat cancer, especially leukemia. A variety of chemicals stimulates differentiation of leukemic cells, such as dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA). Tumor suppressor genes have a vital role in the gateway to terminal cell differentiation. In this study, we inhibited PTEN tumor suppressor gene expression by siRNA to investigate the effect of potentiating cell survival and inhibiting apoptosis on HL-60 cell differentiation by DMSO and ATRA...
October 2016: DNA and Cell Biology
Olivia C Ihedioha, Robert P C Shiu, Jude E Uzonna, Yvonne Myal
The propensity for breast cancers to elicit immune responses in patients is well established. The accumulation of tumor infiltrating lymphocytes within the primary breast tumor has been linked to better prognosis and better response to therapy. The prolactin-inducible protein (PIP) is a 15 kD protein that is expressed under physiological conditions of the breast and is regarded as a marker of mammary differentiation. While highly expressed under pathological conditions of the mammary gland, including breast cancers, PIP is expressed in very few other cancers...
October 2016: DNA and Cell Biology
Rong Hu, Xianhua Dai, Zhiming Dai, Qian Xiang, Yanning Cai
To model quantitatively embryonic stem cell (ESC) self-renewal and differentiation by computational approaches, we developed a unified mathematical model for gene expression involved in cell fate choices. Our quantitative model comprised ESC master regulators and lineage-specific pivotal genes. It took the factors of multiple pathways as input and computed expression as a function of intrinsic transcription factors, extrinsic cues, epigenetic modifications, and antagonism between ESC master regulators and lineage-specific pivotal genes...
October 2016: DNA and Cell Biology
Carlos R Zárate-Bladés, Reiko Horai, Rachel R Caspi
Intestinal microbes have profound effects on inflammatory autoimmunity in sites distant from the gut. The mechanisms whereby this happens are only now beginning to be understood and may include such diverse effects as innate stimulation of migrating immune cells and effects of circulating bacterial metabolites. Our studies add to this the demonstration that microbiota may provide a source of cross-reactive antigenic material that activates autoreactive lymphocytes within the gut environment. In a spontaneous model of autoimmune uveitis, T lymphocytes specific to a retinal autoantigen are activated through their specific antigen receptor in the gut and acquire the ability to fuel inflammatory autoimmunity in the eye...
September 2016: DNA and Cell Biology
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