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DNA and Cell Biology

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https://www.readbyqxmd.com/read/28092463/ccaat-enhancer-binding-protein-%C3%AE-mediates-the-killing-of-toxoplasma-gondii-by-inducing-autophagy-in-nonhematopoietic-cells
#1
Yanhui Yu, Na Zhao, Jiaqi An, Xichen Zhang
Autophagy is a main defense strategy by which infected host cells can virtually induce the killing of parasite, including Toxoplasma gondii. However, the regulatory mechanisms of autophagy in T. gondii-infected nonhematopoietic cells are still unknown. Emerging evidence indicates that CCAAT/enhancer-binding protein β (C/EBP β) is associated with the regulation of autophagy. Herein, we hypothesized that C/EBP β plays roles in inducing autophagy in nonhematopoietic cells. Expression of C/EBP β was aberrantly regulated in endothelial cells and retinal pigment epithelial cells challenged by T...
January 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28085489/microrna-130a-promotes-human-keratinocyte-viability-and-migration-and-inhibits-apoptosis-through-direct-regulation-of-stk40-mediated-nf-%C3%AE%C2%BAb-pathway-and-indirect-regulation-of-sox9-meditated-jnk-mapk-pathway-a-potential-role-in-psoriasis
#2
Ying Xiong, Hongxiao Chen, Liqian Liu, Leihong Lu, Zongshan Wang, Fujun Tian, Yongliang Zhao
Psoriasis is a chronic inflammatory skin disorder. The aim of this study was to determine a potential role of microRNA (miR)-130a in psoriasis, and underlying mechanism. Expression levels of miR-130a in psoriasis specimens and normal skin tissues were analyzed. MiR-130a mimic, inhibitor, miR-control, small interfering RNA (siRNA) specific serine/threonine kinase 40 (STK40), or sex-determining region Y chromosome-box 9 (SOX9) were transfected to human keratinocyte HaCaT cells, respectively. After transfection, the cell viability, apoptosis, and migration were determined...
January 13, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28080131/hypusination-of-eif5a-as-a-target-for-antiviral-therapy
#3
Michelle E Olsen, John H Connor
No abstract text is available yet for this article.
January 12, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28075173/tgf%C3%AE-1-induced-lncrna-uca1-upregulation-promotes-gastric-cancer-invasion-and-migration
#4
Zhong-Kun Zuo, Yi Gong, Xiang-Heng Chen, Fei Ye, Zheng-Ming Yin, Qian-Ni Gong, Jiang-Sheng Huang
According to recent studies, long noncoding RNA urothelial carcinoma associated 1 (UCA1) is involved in the development and progression of many malignant tumors, including gastric cancer (GC). We validated the detailed role of UCA1 in human GC cell lines and GC tissues so as to determine its exact function and the underlying mechanism of GC invasion and migration. In our research, lncRNA-UCA1 was specifically upregulated in GC tissues and cell lines, and augmented GC cell proliferation, and invasive and migratory capabilities...
January 11, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28060535/circulating-micrornas-promising-biomarkers-involved-in-several-cancers-and-other-diseases
#5
Yicheng Zhao, Yuanyuan Song, Li Yao, Guangqi Song, Chunbo Teng
Recently, many studies indicated that microRNAs (miRNAs) stably existed in various body fluids, including serum, plasma, saliva, and urine. Such miRNAs that exist in mammalian body fluids are known as circulating miRNAs, and they can transmit signals between cells and regulate intracellular gene expression. Currently, we barely understand the characteristics, sources, secretion, uptake, and functions of newly generated miRNAs. Particularly, it has been shown that certain types of circulating miRNAs can provide effective clinical data, suggesting their roles as novel biomarkers for the early detection of diseases such as cancers, cardiovascular diseases, and diabetes...
January 6, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28055236/characterization-of-vntrs-within-the-entire-region-of-slc6a3-and-its-association-with-hypertension
#6
Won-Tae Kim, Se-Ra Lee, Yun-Gil Roh, Seung Il Kim, Yung Hyun Choi, Mi-Hye Mun, Mi-So Jeong, Sang Seok Koh, Sun-Hee Leem
The dopamine transporter SLC6A3 (DAT1) mediates uptake of dopamine into presynaptic terminals. In addition, in previous reports, hypertensive rats were associated with DAT gene, but the genetic association with SLC6A3 and hypertension is still unknown. We examined the distribution of variable number of tandem repeats (VNTRs) and conducted polymorphic analysis of the entire region of SLC6A3. Ten VNTR regions (MS1-10) were revealed throughout the intronic and UTRs; seven VNTR regions were newly isolated and three VNTRs were previously reported...
January 5, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28055232/biological-features-of-a-renal-cell-carcinoma-cell-line-derived-from-spinal-metastasis
#7
Zhenhua Zhou, Yan Li, Huiqing Wang, Xudong Wang, Jianru Xiao
The establishment of a metastatic renal cell carcinoma (mRCC) cell line can facilitate the search for molecular mechanisms involved in RCC metastasis. A novel human mRCC cell line, designated RCC96, was established from an mRCC of the spine from a 65-year-old Chinese man. Morphology, cell cycle phase, chromosome number, cell capability of migration, tumorigenicity in nude mice, and cytogenetic features of RCC96 were investigated. Cell growth curve was detected and the cell number doubling time was 52 h. Karyotype analysis showed that these cells were polyploidy...
January 5, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28051330/g-protein-alpha-s-subunit-promotes-cell-proliferation-of-renal-cell-carcinoma-with-involvement-of-protein-kinase-a-signaling
#8
Bo Zhang, Nan Sun, Xin Mu, Lei Zhi, Lei Zhai, Yuan Jiang, Zheng Fu, Zhi Yao
Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main transcript encoded by the GNAS locus) and its biological function in renal cell carcinoma (RCC). Western blotting and quantitative reverse transcription-PCR results show that Gαs expression dramatically increased in RCC cell lines (ACHN, GRC-1, and 786-O) compared to normal renal epithelial cells HK-2...
January 4, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28005398/the-paracrine-effect-of-degenerated-disc-cells-on-healthy-human-nucleus-pulposus-cells-is-mediated-by-mapk-and-nf-%C3%AE%C2%BAb-pathways-and-can-be-reduced-by-tgf-%C3%AE-1
#9
Feng Cai, Lei Zhu, Feng Wang, Rui Shi, Xin-Hui Xie, Xin Hong, Xiao-Hu Wang, Xiao-Tao Wu
Inflammation is thought to have a major role in the pathogenesis of disc degeneration. Studies have shown that nucleus pulposus cells (NPCs) respond to one or two specific cytokines by regulating cell proliferation or matrix synthesis. However, the effects of a cocktail of factors secreted by degenerated disc cells on transplanted exogenous healthy NPCs remain unknown. Concentrations of multiple cytokines in degenerated disc tissue-conditioned medium (dCM) were measured using enzyme-linked immunosorbent assay (ELISA)...
December 22, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/28004979/epigenetic-silencing-of-the-human-18%C3%A2-kda-translocator-protein-in-a-t-cell-leukemia-cell-line
#10
Ryan J Middleton, Winnie Wai-Ying Kam, Guo-Jun Liu, Richard B Banati
The mitochondrial membrane 18 kDa translocator protein (TSPO), previously known as the peripheral benzodiazepine receptor, is constitutively expressed in most organs, most abundantly in hormonal tissue and cells of mononuclear phagocyte lineage, while in the brain, TSPO expression is induced in the wake of injury, inflammation, and neurodegeneration. Increased TSPO expression is also prominent in several cancerous tissues where it appears to correlate with the degree of malignancy. Currently, TSPO is thus actively investigated as a generic biomarker for disease activity and a therapeutic target for a wide range of diseases...
December 22, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27982695/17-dmag-protects-against-hypoxia-reoxygenation-induced-cell-injury-in-ht22-cells-through-akt-nrf2-ho-1-pathway
#11
Zhuopeng Jia, Arui Dong, Hongmin Che, Yu Zhang
Oxidative stress is well known to play a pivotal role in hypoxia/reoxygenation (H/R)-induced neuron injury. On the basis of this fact, antioxidative agents have been demonstrated to be neuroprotective. 17-DMAG (HSP90 inhibitor) is reported to have neuroprotective effects in vitro, which may interfere with oxidative stress through reduction in pro-oxidative factors. However, little is known about its effects on H/R-induced neuron injury and the underlying mechanisms. In this study, the effects of 17-DMAG on H/R-treated HT22 cells were investigated...
December 16, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27982693/long-noncoding-rna-dancr-is-a-positive-regulator-of-proliferation-and-chondrogenic-differentiation-in-human-synovium-derived-stem-cells
#12
Lei Zhang, Chao Yang, Shuo Chen, Guihua Wang, Ben Shi, Xin Tao, Liwu Zhou, Jianning Zhao
Cartilage tissues have limited capacity for repair after damage and then cause osteoarthritis, so finding alternative treatment is ongoing. Mesenchymal stem cells (MSCs) have become a promising therapy for cartilage damage and diseases due to the advantages of easy separation, high proliferative potentiality, and genetic stability. Synovium-derived MSCs (SMSCs) have been recognized as an ideal source for cartilage repair. In our previous study, we found that Sox4 promoted proliferation and chondrogenesis of SMSCs through upregulation of long noncoding RNA (lncRNA) DANCR...
December 16, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27977308/zika-and-flaviviruses-phylogeny-based-on-the-alignment-free-natural-vector-method
#13
Yongkun Li, Lily He, Rong Lucy He, Stephen S-T Yau
Zika virus (ZIKV) is a mosquito-borne flavivirus. It was first isolated from Uganda in 1947 and has become an emergent event since 2007. However, because of the inconsistency of alignment methods, the evolution of ZIKV remains poorly understood. In this study, we first use the complete protein and an alignment-free method to build a phylogenetic tree of 87 Zika strains in which Asian, East African, and West African lineages are characterized. We also use the NS5 protein to construct the genetic relationship among 44 Zika strains...
December 15, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27929351/expression-of-the-cardiac-maintenance-and-survival-factor-fgf-16-gene-is-regulated-by-csx-nkx2-5-and-is-an-early-target-of-doxorubicin-cardiotoxicity
#14
Jie Wang, Yan Jin, Peter A Cattini
The fibroblast growth factor (FGF) 16 gene (Fgf-16) is preferentially expressed by neonatal cardiomyocytes after birth, with levels increasing into adulthood. Null mice and isolated heart studies suggest a role for FGF-16 in cardiac maintenance and survival, including increased resistance to doxorubicin (DOX)-induced injury. However, the effect of DOX on endogenous FGF-16 synthesis and specifically regulation of cardiac Fgf-16 expression has not been reported. Here we assess the effect of DOX on FGF-16 RNA levels and stability as well as promoter activity and use sequence analysis, knockdown, and overexpression to investigate the role of cardiac transcription factor(s) implicated in the response...
December 8, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27911586/mirna-181a-b-regulates-phenotypes-of-vessel-smooth-muscle-cells-through-serum-response-factor
#15
Xiaoxing Wei, Xue Hou, Jianhua Li, Yongnian Liu
The phenotypic modulation of vessel smooth muscle cells (VSMCs) plays a crucial role in the physiological and pathological conditions of vasculature in response to local environmental changes. The phenotypic transition of VSMCs is largely modulated by the serum response factor (SRF). MiR-181a and miR-181b are members of the well-studied miR-181 family and both have complementary sequence in the 3' untranslated region (UTR) of SRF gene. In this article, evidence insinuates that miR-181a/b was involved in VSMCs differentiation through upregulating synthetic marker genes and downregulating contractile ones, respectively...
December 2, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/28045567/disruption-of-the-gaa-gene-in-zebrafish-fails-to-generate-the-phenotype-of-classical-pompe-disease
#16
Jing Wu, Yi Yang, Chengjun Sun, Shaoyang Sun, Qiang Li, Yuxiao Yao, Fei Fei, Lingeng Lu, Zhuo Chang, Wenting Zhang, Xu Wang, Feihong Luo
The underlying pathogenic lesions of glycogen storage disease type II (GSD II) and the diversity of this disease among different species are still under exploration. Thus, we created an acid alpha-glucosidase (gaa) gene-mutated zebrafish model of GSD II and examined the sequential pathogenic changes. gaa mRNA and protein expression, enzymatic activity, and lysosomal glycogen accumulation were assessed, and the phenotypic changes were compared between wild-type (WT) and gaa-mutated zebrafish. The presence of a Δ13 frameshift mutation in the gaa gene was confirmed at both the DNA and transcribed mRNA levels by Sanger sequencing...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28004977/establishment-of-transgenic-porcine-fibroblasts-expressing-a-human-klotho-gene-and-its-effects-on-gene-expression-and-preimplantation-development-of-cloned-embryos
#17
Sanghoon Lee, Joon Ho Moon, Kilyoung Song, Anukul Taweechaipaisankul, Young Kwang Jo, Hyun Ju Oh, Se Chang Park, Byeong Chun Lee
Even though the functions of the klotho gene in aging of small animals such as mice have been well investigated, studies using large animal models such as pigs, which have many similarities to humans, have been limited due to the absence of cell lines or animal models. Therefore, the objective of this study was to generate porcine cell lines overexpressing human klotho (hKlotho) and tetracycline (Tet)-inducible hKlotho and to produce cloned embryos from these cell lines. We designed vectors for hKlotho overexpression (CA-Klotho) under control of CMV enhancer/chicken β-actin (CAG) promoter and Tet-inducible hKlotho overexpression (Tet-Klotho, under control of doxycycline-dependent promoter)...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/27918674/mir-224-affects-mammary-epithelial-cell-apoptosis-and-triglyceride-production-by-downregulating-acadm-and-aldh2-genes
#18
Binglei Shen, Qiqi Pan, Yuwei Yang, Yan Gao, Xin Liu, Wei Li, Yunsheng Han, Xue Yuan, Yongli Qu, Zhihui Zhao
MicroRNAs (miRNAs) are small noncoding RNA molecules that involve in various biological functions by regulating the expressions of target genes. In recent years, many researchers have demonstrated that miR-224 played an important role in regulating lipid metabolism. Therefore, in this study, the target genes of miR-224 were verified and the regulatory role of miR-224 was confirmed in lipid metabolism. In this study, bioinformatics methods were used for primarily predicting the target gene of miR-224 and dual-luciferase reporter system was used for further verify the relationship between miR-224 and its target gene...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/27893280/establishment-of-a-human-breast-cancer-model-by-fusion-pcr-for-in-vivo-and-in-vitro-fluorescence-imaging-of-human-breast-cancer
#19
Can Huang, Wenjun Lan, Feifei Wang, Chun Zhang, Xiaomei Liu, Qin Chen
This study aimed to construct a breast cancer model that could continuously express the genes of luciferase and green fluorescent protein. The genes luciferase, EGFP, and Neo were obtained by fusion polymerase chain reaction (PCR) and inserted into pAAV-MCS. The pAAV-Luciferase-EGFP-Neo vector was transfected into MDA-MB-231 cells. After antibiotic resistance gene screening and limiting dilution assay, we constructed a monoclonal stable cell line that expresses the fusion protein Luciferase-EGFP. In comparison with the polyclonal stable cell line, the monoclonal cell line had good genetic stability and was not different from the parental cell line MDA-MB-231...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/27854515/lncrna-uca1-contributes-to-imatinib-resistance-by-acting-as-a-cerna-against-mir-16-in-chronic-myeloid-leukemia-cells
#20
Yun Xiao, Changjie Jiao, Yiqiang Lin, Meijun Chen, Jingwen Zhang, Jiajia Wang, Zhongying Zhang
Imatinib (IM) has been applied to the chronic phase of chronic myeloid leukemia (CML) and has great benefit on the prognosis of patients with CML. The function of drug efflux mediated by multidrug resistance protein-1 (MDR1) is considered as a main reason for IM drug resistance in CML cells. However, the exact mechanisms of MDR1 modulation in IM resistance of CML cells remain unclear. In the present study, long noncoding RNA (lncRNA) UCA1 was identified as an important modulator of MDR1 by a model system of leukemia cell lines with a gradual increase of MDR1 expression and IM resistance...
January 2017: DNA and Cell Biology
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