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DNA and Cell Biology

Syed Fawad Ali Shah, Tahir Iqbal, Raheel Qamar, Muhammad Arshad Rafiq, Sabir Hussain
The purpose of this study is to investigate the association of variant alleles (rs2781666 and rs2781667) at ARG1 to be involved in the generation of essential hypertension (EH) phenotypes in human subjects. The ARG1 noncoding polymorphisms (rs2781666; Chr6:131572419-G/T and rs2781667; Chr6:131573754-C/T) were investigated in 570 subjects, including 285 individuals diagnosed with EH. Determination of serum arginase activity and concentrations of nitric oxide catabolites were detected by the colorimetric enzymatic assay...
May 14, 2018: DNA and Cell Biology
Jingyi Yuan, Jiali Zeng, Chun Shuai, Yue Liu
Gastric cancer has a high incidence and mortality in the world, especially in China. The pathogenesis leading to the high heterogeneity of gastric cancer remains unclear. It is believed that TWSG1 is associated with a variety of tumors, but there are few studies related to gastric cancer. To investigate the biological significance of TWSG1, we evaluated the TWSG1 expression of the clinical samples and gastric cancer cell lines (BGC-823, MGC-803, and SGC-790) via stomach cancer tissue array, real-time PCR, and western blotting...
May 14, 2018: DNA and Cell Biology
Mary R Doherty, Mark W Jackson
Triple-negative breast cancer (TNBC) the deadliest form of this disease currently lacks a targeted therapy and is characterized by increased risk of metastasis and presence of therapeutically resistant cancer stem cells (CSC). Recent evidence has demonstrated that the presence of an interferon (IFN)/signal transducer of activated transcription 1 (STAT1) gene signature correlates with improved therapeutic response and overall survival in TNBC patients. In agreement with these clinical observations, our recent work has demonstrated, in a cell model of TNBC that CSC have intrinsically repressed IFN signaling...
May 11, 2018: DNA and Cell Biology
Juan Ramos-Treviño, Susana Bassol-Mayagoitia, José Anselmo Hernández-Ibarra, Pablo Ruiz-Flores, Martha P Nava-Hernández
Over the past decades, an increase has been described in exposure to environmental toxins; consequently, a series of studies has been carried out with the aim of identifying problems associated with health. One of the main risk factors is exposure to heavy metals. The adverse effects that these compounds exert on health are quite complex and difficult to elucidate, in that they act at different levels and there are various signaling pathways that are implicated in the mechanisms of damage. The Sertoli cells plays a role of vital importance during the process of spermatogenesis, and it has been identified as one of the principal targets of heavy metals...
May 10, 2018: DNA and Cell Biology
Gustavo Mendonça André, Camila Martins Trevisan, Isabela Nacione Pedruzzi, Ramon Felix Martins Fernandes, Renato Oliveira, Denise Maria Christofolini, Bianca Bianco, Caio Parente Barbosa
Endometriosis is an estrogen-dependent inflammatory disease that affects a large number of women in reproductive age. Follicle-stimulating hormone (FSH) plays a role in steroidogenesis and acts through a transmembrane glycoprotein, FSH receptor (FSHR). Polymorphisms in FSHR gene were previously associated with variability in FSH serum level and reproductive outcomes, but its relation with endometriosis has not been clarified and demonstrated conflicting results, ranging from strong links to no association to endometriosis...
April 23, 2018: DNA and Cell Biology
Le Zhang, Yue Ning, Peiwei Li, Hongfang Guo, Linsen Zan
The transforming growth factor-β (TGFβ) pathway plays many key roles in regulating numerous biological processes. In addition, the effects of TGFβ are mediated by the transcription factor Smad3. However, the regulation of Smad3 activity is not well understood. In the present study, quantitative real-time PCR revealed that the Smad3 gene was expressed ubiquitously in 11 bovine tissues and displayed different expression patterns between muscle and adipose tissue. We further explored the expression and regulation of Smad3 gene by cloning the bovine Smad3 gene promoter; a dual-luciferase reporter assay identified that the core promoter region -337 to -41 bp was located in a CpG island...
April 19, 2018: DNA and Cell Biology
Seyed Abbas Mirzaei, Mansureh Safari Kavishahi, Zhila Keshavarz, Fatemeh Elahian
The search for new chemotherapeutics unaffected by efflux pumps would significantly increase life expectancy in patients with malignant cancers. In this study, butylcycloheptylprodigiosin and undecylprodigiosin were HPLC-purified and verified, using nuclear magnetic resonance spectroscopy. Cell cytotoxicity and transportation kinetics on multiple-drug resistance (MDR) cells were evaluated. Daunorubicin and butylcycloheptylprodigiosin were less toxic in the MDR1 overexpressing line, but undecylprodigiosin revealed potent toxicity toward MDR1 and BCRP expressing malignant cells...
April 19, 2018: DNA and Cell Biology
Yami Liu, Wen Peng, Kai Qu, Xiaolong Lin, Zhaolin Zeng, Jiaojiao Chen, Dangheng Wei, Zuo Wang
Atherosclerosis is the underlying cause of cardio-cerebrovascular disease. However, the mechanisms of atherosclerosis are still unclear. The modification of DNA methylation has an important role in atherosclerosis development. As a member of the Ten-eleven translocation (TET) family, TET methylcytosine dioxygenase 2 (TET2) can modify DNA methylation by catalyzing 5-methylcytosine to 5-hydroxymethylcytosine and mediate DNA demethylation. Recent findings suggest that TET2 is related to the phenotype transformation of vascular smooth muscle cells, endothelial dysfunction, and inflammation of macrophage, the key factors of atherosclerosis...
April 13, 2018: DNA and Cell Biology
Nasha Zhang, Youhua Lu, Xijun Liu, Dianke Yu, Zheng Lv, Ming Yang
ZNF350, a BRCA1-interacting protein, could mediate BRCA1-induced sequence-specific transcriptional repression of several genes, including GADD45α. As a potential breast cancer susceptibility gene, single nucleotide polymorphisms (SNPs), especially missense SNPs, may influence the transcriptional repression of its target tumor suppressor genes and individuals' breast cancer risk. Using the gene-based haplotype-tagging SNPs strategy, we evaluated the association between six ZNF350 polymorphisms and breast cancer risk in a case-control set from a northern Chinese population...
April 13, 2018: DNA and Cell Biology
Xuan Shu, Shenyou Shu, Hongqiu Cheng, Shijie Tang, Lujun Yang, Haihong Li, Mingjun Zhang, Zhensen Zhu, Dan Liu, Ke Li, Zejun Dong, Liuhanghang Cheng, Jialong Ding
Epithelial mesenchyme transformation (EMT) of the medial edge epithelium (MEE) is the crucial process during palatal fusion. This work is aimed to elucidate the enhancer regulatory mechanism by genome-wide DNA methylation analysis of EMT during palatal fusion. Over 800 million clean reads, 325 million enzyme reads, and 234 million mapping reads were generated. The mapping rate was 68.85-74.32%, which included differentially methylated 17299 CCGG sites and 2363 CCWGG sites (p < 0.05, log2 FC >1). Methylated sites in intron and intergenic regions were more compared to other regions of all DNA elements...
April 2, 2018: DNA and Cell Biology
Qin Li, Yong Lei, Wei Du
Uterine corpus endometrial carcinoma (UCEC) is a common malignancy in the female reproductive system, associated with high morbidity and mortality. Despite the high prevalence of UCEC, molecular understanding of uterine endothelium tumorigenesis remains poorly understood. In this study, we reported that transcription factor 21 (TCF21) inhibits cancer cell proliferation and invasion following overexpression, in vitro and in vivo. Moreover, in response to hypoxia, TCF21 is highly expressed in UCEC cells carrying wild-type p53, and is transcriptional target of p53...
April 2, 2018: DNA and Cell Biology
Himanshu V Patankar, Ibtisam Al-Harrasi, Rashid Al-Yahyai, Mahmoud W Yaish
Although date palm is a relatively salt-tolerant plant, the molecular basis of this tolerance is complex and poorly understood. Therefore, this study aimed to identify the genes involved in salinity tolerance using a basic yeast functional bioassay. To achieve this, a date palm cDNA library was overexpressed in Saccharomyces cerevisiae cells. The expression levels of selected genes that make yeast cells tolerant to salt were subsequently validated in the leaf and root tissues of date palm seedlings using a quantitative PCR method...
March 29, 2018: DNA and Cell Biology
Erkun Guo, Chaohui Liang, Xin He, Guozhi Song, Hongjiang Liu, Zhongqiang Lv, Jianchao Guan, Dezhen Yang, Jiapeng Zheng
Increasing evidence has indicated that long noncoding RNAs (lncRNAs) play crucial roles in various biological processes, including glioma. However, the underlying mechanism of lncRNAs in gliomagenesis is still ambiguous. In this study, we aim to investigate the role of long intergenic noncoding RNA 00958 (LINC00958) in the tumorigenesis of glioma. Results revealed that LINC00958 was significantly upregulated in glioma tissues and cell lines compared with that of adjacent normal brain tissues and normal human astrocytes...
March 23, 2018: DNA and Cell Biology
Hua Wang, Shu Zhang, Aihong Zhang, Cunling Yan
This study aimed to explore the efficacy of propofol to treat malignant pheochromocytoma (PCC) in vitro and in vivo. In vitro, PC12 cells were treated with different concentrations of propofol (0, 1, 5, and 10 μg/mL) for specific times followed by a MTT assay to detect cell proliferation. Transwell assays were performed to assess the function of propofol on the migration and invasion of PC12 cells, and flow cytometry to analyze cell apoptosis and cell cycle progression. Quantitative real-time polymerase chain reaction was carried out to analyze the expression level of mRNA (Bcl-2, Bax, and CyclinE)...
March 22, 2018: DNA and Cell Biology
Jihong Yang, Jinghua Li, Bo Liu, Rui Zhang, Feng Gu, Jisen Zhao, Shujie Cheng
Long noncoding RNAs (lncRNAs) were dysregulated in many kinds of cancers, including hepatocellular carcinoma (HCC). AK021443, as a novel lncRNA, was found to be upregulated in HCC, while its potential value and function are still unknown. The pathological changes of liver tissues were observed by hematoxylin and eosin staining. The expression levels of AK021443 in HCC tissues and cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation ability of AK021443 on HepG2 and Bel-7402 cells was assessed by CCK8 and EdU staining assays...
March 14, 2018: DNA and Cell Biology
Arnold Park, Guoyan Zhao
Type 1 diabetes (T1D) is characterized by the autoimmune destruction of insulin-producing pancreatic beta cells. Although environmental factors interplay with genetic susceptibility to promote immune dysregulation and disease, it remains unclear as to which potential environmental factors are causative and not simply correlative. Despite many hints that the microbiome can have a profound effect on T1D, significant changes in bacterial gut flora and diversity appear to emerge only after the detection of early signs of T1D...
March 13, 2018: DNA and Cell Biology
Lei Gong, Mingyang Ren, Zhenbing Lv, Yuling Yang, Ziwei Wang
microRNA (miR)-92b is an oncogenic miRNA. F-box and WD-40 domain protein 7 (FBXW7/hCdc4) is a tumor suppressor and a target of miR-92b-3p. This study was designed to investigate the effect of miR-92b-3p on colorectal carcinoma (CRC) invasion. The expression levels of miR-92b-3p in human HT29, HCT116, and human fetal colon (FHC) normal cells were detected. HT29 and HCT116 cells were transfected with either miR-92b-3p inhibitor or FBXW7 expression plasmids (pcDNA-FBXW7) and combination of miR-92b-3p and siRNA-FBXW7...
March 9, 2018: DNA and Cell Biology
Xiukun Cui, Zheng Zhou, Keke Zhu, Ruiping Feng, Jiuli Han, Mengyuan Li, Shuangfeng Wang, Jing Li, Jing Zhang, Qiying Jiang, Wanting Zhang, Hongmei Mu, Yutao Liu, Yanzhong Hu
Mutations in GJA8 are associated with hereditary autosomal dominant and recessive cataract formation. In this study, a novel insert mutation in GJA8 was identified in a Chinese congenital cataract family and cosegregated with the disease in this pedigree. This insert mutation introduces five additional amino acid residues YAVHY after histidine at the 95 site (p.H95_A96insYAVHY) within the second transmembrane (TM2) domain of Cx50 protein (Cx50-insert). Ectopic expression of Cx50-insert protein impairs the hemichannel functions and gap junction activity compared to wild-type Cx50 protein in human lens epithelial cells...
May 2018: DNA and Cell Biology
Xiuxiu Li, Xiaoning Li, Rongrong Luo, Wenwen Wang, Tao Wang, Hui Tang
The dominant white phenotype in domestic pigs is caused by two mutations in the KIT gene: a 450 kb duplication containing the entire KIT gene together with flanking sequences and one splice mutation with a G:A substitution in intron 17. The purpose of this study was to establish a simple, rapid method to determine KIT genotype in pigs. First, to detect KIT copy number variation (CNV), primers for exon 2 of the KIT gene, along with a TaqMan minor groove binder (MGB) probe, were designed. The single-copy gene, estrogen receptor (ESR), was used as an internal control...
May 2018: DNA and Cell Biology
Mei Lin, Bairong Xia, Ling Qin, Hong Chen, Ge Lou
Epithelial ovarian cancer (EOC) is the most common cause of gynecological cancer-associated death. The high mortality rate is largely due to early stage metastasis and posttreatment recurrence. Identifying crucial regulators of EOC cells as well as ways to target them promises to improve the disease's prognosis. The S100 calcium-binding protein A7 (S100A7) has been shown to promote cell proliferation, migration, invasion, and tumor metastasis. In this study, we have investigated the role that S100A7 plays in regulating EOC cells...
May 2018: DNA and Cell Biology
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