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Biologicals: Journal of the International Association of Biological Standardization

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https://www.readbyqxmd.com/read/28222938/the-role-of-mir-17-92-cluster-in-the-expression-of-tumor-suppressor-genes-in-unrestricted-somatic-stem-cells
#1
Rezvan Tavakoli, Saeid Vakilian, Lida Langroudi, Ehsan Arefian, Mehdi Sahmani, Masoud Soleimani, Fatemeh Jamshidi-Adegani
The miR-17-92 cluster consisted of seven miRNAs (mir-17-5p, -17-3p, -18a, -19a, -20a, -19b-1, and -92a-1). Previous studies have shown this cluster has been over-expressed in several cancers. The aim of this study was to evaluate the over-expression impacts of miR-17-92 on stem cells. In the current work, the effect of miR-17-92 cluster which was cloned in Lentiviral vector has been investigated on unrestricted somatic stem cells (USSCs). Tumor suppressor genes (p53, p15, RBL1, SMAD2, SMAD4, and MAPK-1) expression, especially p53, was considerably reduced...
February 17, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28215695/inactivation-of-viruses-by-pasteurization-at-60%C3%A2-%C3%A2-c-for-10%C3%A2-h-with-and-without-40-glucose-as-stabilizer-during-a-new-manufacturing-process-of-%C3%AE-2-macroglobulin-from-cohn-fraction-iv
#2
Chaoji Huangfu, Yuyuan Ma, Junting Jia, Maomin Lv, Fengxuan Zhu, Xiaowei Ma, Xiong Zhao, Jingang Zhang
Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28215694/comparisons-of-the-humoral-and-cellular-immunity-induced-by-live-a16r-attenuated-spore-and-ava-like-anthrax-vaccine-in-mice
#3
Jin Lv, Ying-Ying Zhang, Xun Lu, Hao Zhang, Lin Wei, Jun Gao, Bin Hu, Wen-Wei Hu, Dun-Zhong Hu, Na Jia, Xin Feng
The live attenuated anthrax vaccine and anthrax vaccine adsorbed (AVA) are two main types of anthrax vaccines currently used in human. However, the immunoprotective mechanisms are not fully understood. In this study, we compared humoral and cellular immunity induced by live A16R spore vaccine and A16R strain derived AVA-like vaccine in mice peripheral blood, spleen and bone marrow. Both A16R spores and AVA-like vaccines induced a sustained IgG antibody response with IgG1/IgG2b subtype dominance. However, A16R spores vaccine induced higher titer of IgG2a compared with AVA-like vaccine, indicating a stronger Th1 response to A16R spores...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28214171/g-protein-based-elisa-as-a-potency-test-for-rabies-vaccines
#4
Martine Chabaud-Riou, Nadège Moreno, Fabien Guinchard, Marie Claire Nicolai, Elisabeth Niogret-Siohan, Nicolas Sève, Catherine Manin, Françoise Guinet-Morlot, Patrice Riou
The NIH test is currently used to assess the potency of rabies vaccine, a key criterion for vaccine release. This test is based on mice immunization followed by intracerebral viral challenge. As part of global efforts to reduce animal experimentation and in the framework of the development of Sanofi Pasteur next generation, highly-purified vaccine, produced without any material of human or animal origin, we developed an ELISA as an alternative to the NIH test. This ELISA is based on monoclonal antibodies recognizing specifically the native form of the viral G-protein, the major antigen that induces neutralizing antibody response to rabies virus...
February 14, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28209271/a-simple-method-of-extracting-cap-antigen-of-pcv2b-from-emulsified-vaccines-for-testing-its-stability-and-antigenicity
#5
Cheng Yu, Xin Li, Wenzhen Diao, Jiwei Liu, Yunpeng Yao, Li Hua, Yaqin Yu, Yongli Yu, Liying Wang
Oil-based emulsions are commonly used adjuvants for veterinary vaccines. After formulation, it is required to extract protein antigens from emulsified vaccines for testing their stability and antigenicity. To establish a simple method to extract the protein antigens, two emulsified vaccines, designated as Cap-206 and Cap-35, were prepared by formulating a 28-kDa capsid protein (Cap) of PCV2b with ISA 206, a water-in-oil-in-water emulsion, or ISA 35, an oil-in-water emulsion. We found that the freeze-thaw-centrifugation method with steps of freezing at -20 °C for 12 h, thawing at room temperature and centrifuging at 9000×g for 10 min could separate the aqueous phase from Cap-206, and a centrifugation method by centrifuging at 9000×g for 10 min could isolate the aqueous portion from Cap-35...
February 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28189484/expression-purification-and-evaluation-of-recombinant-lipoprotein-of-salmonella-typhi-as-a-vaccine-candidate
#6
Anand Kumar, Subir Kundu, Mira Debnath Das
Lipoprotein has been reported as a vaccine candidate against many pathogenic bacteria, it plays direct role as a virulence-associated function. Here the approach is toward the expression of recombinant lipoprotein of Salmonella typhi in prokaryotic host and its evaluation as a vaccine candidate. Lipoprotein gene (lp1) was cloned in pET32a expression vector in addition to Bam HI and Hind III restriction sites, and BL21(pLysS) was used as prokaryotic expression host for transformation. Lipoprotein induction was performed by IPTG and 55 kDa (31 kDa of Gene +24 kDa of vector additional protein with His-tag) was analyzed by 12% SDS-PAGE...
February 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28189483/electrical-stimulation-of-somatic-human-stem-cells-mediated-by-composite-containing-conductive-nanofibers-for-ligament-regeneration
#7
Masumeh Dodel, Nahid Hemmati Nejad, S Hajir Bahrami, Masoud Soleimani, Leila Mohammadi Amirabad, Hana Hanaee-Ahvaz, Amir Atashi
One of the advances in the field of biomedical nanotechnology, is conductive nanofiber fabrication and the discovery of its applications. Biocompatible flexible nanofibers that have a good biocompatibility, mechanical properties and morphology. Poly (3, 4-ethylene dioxythiophene) (PEDOT) is a conductive polymer that has recently been used in medical applications. In this study, the electrospinning technique and vapor phase polymerization combination method with freeze drying was used to produce Silk fibroin/PEDOT/Chitosan nanocomposite scaffold...
February 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28173977/identification-of-a-candidate-standard-strain-of-severe-fever-with-thrombocytopenia-syndrome-virus-for-vaccine-quality-control-in-china-using-a-cross-neutralization-assay
#8
Zheng Jia, Xiaohong Wu, Ling Wang, Xiuling Li, Xinxian Dai, Mifang Liang, Shouchun Cao, Yan Kong, Jingjing Liu, Yuhua Li, Junzhi Wang
Severe fever with thrombocytopenia syndrome (SFTS) is caused by a phlebovirus of the Bunyaviridae family, which is designated as SFTS virus (SFTSV). To our knowledge, no efficient SFTSV vaccine exists. Here, we report the identification of a standard virus strain for the eight major SFTSV strains circulating in China for use in evaluating the SFTSV vaccine. Rabbits were immunized with the SFTSV strains and the cross-neutralization capacities of SFTSV anti-sera were determined in microculture cytopathic effect (CPE)-inhibition assays...
February 4, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28169066/standardization-and-validation-of-simple-pcr-duplex-pcr-and-rapd-in-comparison-to-blood-smear-examination-for-diagnosing-bovine-tropical-theileriosis
#9
Vikrant Sudan, Daya Shanker, Amit Jaiswal, Amit Singh, Vijay Pandey
Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle...
February 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28162840/establishment-of-a-reference-material-for-standardization-of-the-anti-complementary-activity-test-in-intravenous-immunoglobulin-products-used-in-japan-a-collaborative-study
#10
Kiyoko Nojima, Kazu Okuma, Masaki Ochiai, Madoka Kuramitsu, Kenta Tezuka, Mieko Ishii, Sadao Ueda, Takashi Miyamoto, Koichiro Kamimura, Enki Kou, Sanae Uchida, Yoshiharu Watanabe, Yoshiaki Okada, Isao Hamaguchi
Aggregates of human plasma-derived intravenous immunoglobulins (IVIGs) carries a risk of severe adverse events after nonspecific complement activation induced in humans administrated. Therefore, the anti-complementary activity (ACA) test is legally required in every batch of IVIGs in Japan. However, due to the intrinsic nature of this bioassay, there might be large differences in the results of ACA tests from laboratories, even when the same batch of IVIGs was measured. Our six laboratories evaluated whether there were such differences and argued for establishment of a reference material (RM) for standardization of the ACA test...
February 2, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28131552/comparative-analysis-of-the-feline-immunoglobulin-repertoire
#11
Sebastian C J Steiniger, Jacob Glanville, Douglas W Harris, Thomas L Wilson, Gregory C Ippolito, Steven A Dunham
Next-Generation Sequencing combined with bioinformatics is a powerful tool for analyzing the large number of DNA sequences present in the expressed antibody repertoire and these data sets can be used to advance a number of research areas including antibody discovery and engineering. The accurate measurement of the immune repertoire sequence composition, diversity and abundance is important for understanding the repertoire response in infections, vaccinations and cancer immunology and could also be useful for elucidating novel molecular targets...
January 25, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28122669/active-immunization-of-cattle-with-a-bothropic-toxoid-does-not-abrogate-envenomation-by-bothrops-asper-venom-but-increases-the-likelihood-of-survival
#12
María Herrera, Katherine González, Carlos Rodríguez, Aarón Gómez, Álvaro Segura, Mariángela Vargas, Mauren Villalta, Ricardo Estrada, Guillermo León
This study assessed the protective effect of active immunization of cattle to prevent the envenomation induced by B. asper venom. Two groups of oxen were immunized with a bothropic toxoid and challenged by an intramuscular injection of either 10 or 50 mg B. asper venom, to induce moderate or severe envenomations, respectively. Non-immunized oxen were used as controls. It was found that immunized oxen developed local edema similar to those observed in non-immunized animals. However, systemic effects were totally prevented in immunized oxen challenged with 10 mg venom, and therefore antivenom treatment was not required...
January 22, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28111083/a-competitive-elisa-for-detection-of-group-specific-antibody-to-bluetongue-virus-using-anti-core-antibody
#13
Karam Chand, Sanchay K Biswas, Awadh B Pandey, Arpit Saxena, Neha Tewari, Bimalendu Mondal
Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA...
January 19, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28108210/comparison-of-various-types-of-collagenous-scaffolds-applied-for-embryonic-nerve-cell-culture
#14
Jacek Drobnik, Krystyna Pietrucha, Marcin Kudzin, Krystyna Mader, Jacek Szymański, Alicja Szczepanowska
The purpose of the study was to confirm whether collagen-based scaffolds using different cross-linking methods are suitable elaborate environments for embryonic nerve cell culture. Three 3D sponge-shaped porous scaffolds were composed using collagen alone, collagen with chondroitin sulphate modified by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride, and collagen cross-linked by 2,3-dialdehyde cellulose (DAC). Embryonic nerve cells from rats were applied to the scaffolds and stained with bisbenzimide to study cell entrapment within the scaffolds...
January 17, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28100412/virome-of-us-bovine-calf-serum
#15
Mohammadreza Sadeghi, Beatrix Kapusinszky, Danielle M Yugo, Tung Gia Phan, Xutao Deng, Isis Kanevsky, Tanja Opriessnig, Amelia R Woolums, David J Hurley, Xiang-Jin Meng, Eric Delwart
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses...
January 15, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28087107/establishment-of-the-2nd-korean-national-biological-reference-standard-for-blood-coagulation-factor-viii-c-concentrate
#16
Naery Lee, Ji Suk Seo, Jae Ok Kim, Sang Ja Ban
Since the 1st Korean national biological reference standard for factor (F)VIII concentrate, established in 2001, has shown declining potency, we conducted this study to replace this standard with a 2nd Korean national biological reference standard for blood coagulation FVIII concentrate. The candidate materials for the 2nd standard were prepared in 8000 vials with 10 IU/ml of target potency, according to the approved manufacturing process of blood coagulation Factor VIII:C Monoclonal Antibody-purified, Freeze-dried Human Blood Coagulation Factor VIII:C...
January 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28087106/charge-variant-analysis-of-proposed-biosimilar-to-trastuzumab
#17
Pravinkumar Dakshinamurthy, Pavithra Mukunda, Bhargav Prasad Kodaganti, Bharath Ravindra Shenoy, Bairavabalakumar Natarajan, Amol Maliwalave, Vivek Halan, Sathyabalan Murugesan, Sunit Maity
Trastuzumab is a humanized monoclonal antibody (mAb) employed for the treatment of HER2 Positive Breast Cancer. A HER2 overexpressing tumor cell binds to Trastuzumab and attracts immune cells which lead to induction of Antibody Dependent Cellular Cytotoxicity (ADCC) by binding to Fc receptors (CD16a or FcγRIIIa) on an effector cell, such as natural killer (NK) cells. The most commonly expressed receptor on NK cell is CD16a which binds to the Fc portion of Trastuzumab. The ligand-independent HER2-HER3 dimerization is the most potent stimulator of downstream pathways for regulation of cell growth and survival...
January 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28082100/a-collaborative-study-to-establish-the-3rd-who-international-standard-for-hepatitis-b-virus-for-nucleic-acid-amplification-techniques
#18
Jacqueline F Fryer, Alan B Heath, Dianna E Wilkinson, Philip D Minor
Nucleic acid amplification techniques (NAT) are routinely used for clinical diagnostics and monitoring hepatitis B virus (HBV) infections, and are implemented on a voluntary basis for blood screening. A collaborative study was performed to evaluate a replacement WHO International Standard for HBV for the standardization of NAT. Two lyophilised HBV candidates were evaluated by 16 laboratories worldwide, alongside the existing HBV International Standard. The overall mean potency estimates for the candidate samples 1 and 2, relative to sample 3 (2nd HBV International Standard), from quantitative assays, were 5...
January 9, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28065582/bivalent-flagellin-immunotherapy-protects-mice-against-pseudomonas-aeruginosa-infections-in-both-acute-pneumonia-and-burn-wound-models
#19
Hosein Ahmadi, Bahador Behrouz, Gholamreza Irajian, Nour Amirmozafari, Sara Naghavi
Pseudomonas aeruginosa infections are a serious challenge to therapy because of the complex pathogenesis and paucity of new effective antibiotics, thus renewing interest in antibody-based therapeutic strategies. Immunotherapy strategies typically target selected virulence factors that are expressed by the majority of clinical strains of P. aeruginosa, particularly because virulence factors mediate infection. Type a and b flagellins (flagellin a+b) of P. aeruginosa are acute virulence factors that play a major role in the establishment of infection...
January 5, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28040390/reduction-of-animal-suffering-in-rabies-vaccine-potency-testing-by-introduction-of-humane-endpoints
#20
Mutsuyo Takayama-Ito, Chang-Kweng Lim, Kazuo Nakamichi, Satsuki Kakiuchi, Madoka Horiya, Guillermo Posadas-Herrera, Ichiro Kurane, Masayuki Saijo
Potency controls of inactivated rabies vaccines for human use are confirmed by the National Institutes of Health challenge test in which lethal infection with severe neurological symptoms should be observed in approximately half of the mice inoculated with the rabies virus. Weight loss, decreased body temperature, and the presence of rabies-associated neurological signs have been proposed as humane endpoints. The potential for reduction of animal suffering by introducing humane endpoints in the potency test for inactivated rabies vaccine for human use was investigated...
December 28, 2016: Biologicals: Journal of the International Association of Biological Standardization
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