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Biologicals: Journal of the International Association of Biological Standardization

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https://www.readbyqxmd.com/read/28284570/enzyme-antibody-dual-film-modified-gold-nanoparticle-probe-for-ultrasensitive-detection-of-alpha-fetoprotein
#1
Qian Xiao, Yizhi Zheng, Jing Liu, Suiping Wang, Bo Feng
In this study, we designed a comprehensive strategy for the ultrasensitive detection of alpha fetoprotein (AFP) with high specificity using gold nanoparticle (AuNP)-based enzyme-linked immunosorbent assay (ELISA). A dual-film modified probe was synthesized by coating AuNPs with horseradish peroxidase (HRP) on its surface. Anti-AFP monoclonal antibody (McAb) was immobilized on the surface of the enzyme using glutaraldehyde cross-linking method. AuNPs, employed as support for the immobilization of HRP. HRP was used not only as the enzymatic-amplified tracer but also as a bridge for loading McAb...
March 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28262479/the-impact-of-oxidative-dna-changes-and-atm-expression-on-morphological-and-functional-activities-on-hepatocytes-obtained-from-mesenchymal-stem-cells
#2
Shahnaz Esmaeli, Abdolamir Allameh, Maryam Adelipour, Masoud Soleimani, Mina Allameh
Resistance to oxidative damages in undifferentiated mesenchymal stem cells (MSCs) in comparison with the undifferentiated progenitor cells may differ depending on several factors. This study was carried out to examine the impact of hepatogenic differentiation process of MSCs on oxidative DNA damage markers. Hepatic differentiation of MSCs was carried out using a two-step conventional protocol and the cells were processed for characterization using morphological and biochemical markers. During the course of differentiation cellular levels of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OH-dG) and expression of ataxia-telangiectasia mutated (ATM) protein were estimated at time intervals (10, 20 and 30 days)...
March 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28259519/antigenic-fingerprinting-of-diphtheria-toxoid-adsorbed-to-aluminium-phosphate
#3
Janny Westdijk, Bernard Metz, Nanda Spruit, Wichard Tilstra, Johan van der Gun, Coenraad Hendriksen, Gideon Kersten
The antigenicity of alum-adsorbed diphtheria toxoid (DTd) was determined in combination vaccines, containing DTd, tetanus toxoid and inactivated poliovirus. A panel of monoclonal antibodies was used, covering five epitopes, distributed over the antigen. The resulting antigenic fingerprint of DTd demonstrates consistency of adsorption at antigen level in final product combination vaccines. The antigenic quality of DTd alone, adsorbed to aluminium phosphate, was also determined and compared with pre-adsorbed toxoid (starting material as well as toxoid desorbed from aluminium phosphate)...
March 1, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28237422/determination-of-pegfilgrastim-aggregates-by-size-exclusion-high-performance-liquid-chromatography-on-a-methacrylate-based-column
#4
Majid Shahbazi, Elnaz Tamaskany Zahedy, Shiva Kiumarsi, Mojtaba Hadi Soltanabad, Saleh Shahbazi Azar, Hossein Amini
A size-exclusion high-performance liquid chromatographic method using a methacrylate-based column was developed, validated and implemented for the determination of pegfilgrastim aggregates. The samples were directly injected into a TSKgel G4000PWXL column (7.5 mm × 300 mm, 10 μm, <500 A°) with a mobile phase of 100 mM phosphate, pH 2.5. Detection was made at 215 nm and analyses were run at a flow-rate of 0.6 ml/min at 10 °C. Vortex-mixing of samples produced oligomers, however, very high molecular weight aggregates were formed at high temperatures...
February 22, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28236560/the-dynamics-of-contract-plasma-fractionation
#5
REVIEW
Albert Farrugia, Daniela Scaramuccia
Plasma Derived Medicinal Products (PMDPs) are an essential component of the modern therapeutic armamentarium. They are differentiated from most other medicines in several ways, particularly the unique nature of the raw material used for their manufacture. Human plasma has been fractionated to PDMPs for the past 75 years, and the economics of manufacturing requires currently that as many products are harvested from each litre as is feasible and reflective of clinical needs. PDMPs may be purchased on the open market from the various commercial and not-for-profit (NFP) manufacturers...
February 21, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28233669/an-inhibition-enzyme-immuno-assay-exploring-recombinant-invariant-surface-glycoprotein-and-monoclonal-antibodies-for-surveillance-of-surra-in-animals
#6
G R Rudramurthy, P P Sengupta, M Ligi, H Rahman
The present study is aimed at the development of inhibition ELISA (I-ELISA) exploring monoclonal antibodies (MAbs) and recombinant invariant surface glycoprotein. The extracellular domain (ED) of invariant surface glycoprotein (ISG-75) from Trypanosoma evasni has been heterologously expressed in Pichia pastoris (X-33). The recombinant ISG-75 (rISG-75ED) was characterized by immunoblot and ELISA, followed by the production of MAbs against rISG-75ED. The MAbs were characterized by immunoblot and then explored in the development of I-ELISA for the detection of surra...
February 20, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28222938/the-role-of-mir-17-92-cluster-in-the-expression-of-tumor-suppressor-genes-in-unrestricted-somatic-stem-cells
#7
Rezvan Tavakoli, Saeid Vakilian, Lida Langroudi, Ehsan Arefian, Mehdi Sahmani, Masoud Soleimani, Fatemeh Jamshidi-Adegani
The miR-17-92 cluster consisted of seven miRNAs (mir-17-5p, -17-3p, -18a, -19a, -20a, -19b-1, and -92a-1). Previous studies have shown this cluster has been over-expressed in several cancers. The aim of this study was to evaluate the over-expression impacts of miR-17-92 on stem cells. In the current work, the effect of miR-17-92 cluster which was cloned in Lentiviral vector has been investigated on unrestricted somatic stem cells (USSCs). Tumor suppressor genes (p53, p15, RBL1, SMAD2, SMAD4, and MAPK-1) expression, especially p53, was considerably reduced...
February 17, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28215695/inactivation-of-viruses-by-pasteurization-at-60%C3%A2-%C3%A2-c-for-10%C3%A2-h-with-and-without-40-glucose-as-stabilizer-during-a-new-manufacturing-process-of-%C3%AE-2-macroglobulin-from-cohn-fraction-iv
#8
Chaoji Huangfu, Yuyuan Ma, Junting Jia, Maomin Lv, Fengxuan Zhu, Xiaowei Ma, Xiong Zhao, Jingang Zhang
Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28215694/comparisons-of-the-humoral-and-cellular-immunity-induced-by-live-a16r-attenuated-spore-and-ava-like-anthrax-vaccine-in-mice
#9
Jin Lv, Ying-Ying Zhang, Xun Lu, Hao Zhang, Lin Wei, Jun Gao, Bin Hu, Wen-Wei Hu, Dun-Zhong Hu, Na Jia, Xin Feng
The live attenuated anthrax vaccine and anthrax vaccine adsorbed (AVA) are two main types of anthrax vaccines currently used in human. However, the immunoprotective mechanisms are not fully understood. In this study, we compared humoral and cellular immunity induced by live A16R spore vaccine and A16R strain derived AVA-like vaccine in mice peripheral blood, spleen and bone marrow. Both A16R spores and AVA-like vaccines induced a sustained IgG antibody response with IgG1/IgG2b subtype dominance. However, A16R spores vaccine induced higher titer of IgG2a compared with AVA-like vaccine, indicating a stronger Th1 response to A16R spores...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28214171/g-protein-based-elisa-as-a-potency-test-for-rabies-vaccines
#10
Martine Chabaud-Riou, Nadège Moreno, Fabien Guinchard, Marie Claire Nicolai, Elisabeth Niogret-Siohan, Nicolas Sève, Catherine Manin, Françoise Guinet-Morlot, Patrice Riou
The NIH test is currently used to assess the potency of rabies vaccine, a key criterion for vaccine release. This test is based on mice immunization followed by intracerebral viral challenge. As part of global efforts to reduce animal experimentation and in the framework of the development of Sanofi Pasteur next generation, highly-purified vaccine, produced without any material of human or animal origin, we developed an ELISA as an alternative to the NIH test. This ELISA is based on monoclonal antibodies recognizing specifically the native form of the viral G-protein, the major antigen that induces neutralizing antibody response to rabies virus...
February 14, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28209271/a-simple-method-of-extracting-cap-antigen-of-pcv2b-from-emulsified-vaccines-for-testing-its-stability-and-antigenicity
#11
Cheng Yu, Xin Li, Wenzhen Diao, Jiwei Liu, Yunpeng Yao, Li Hua, Yaqin Yu, Yongli Yu, Liying Wang
Oil-based emulsions are commonly used adjuvants for veterinary vaccines. After formulation, it is required to extract protein antigens from emulsified vaccines for testing their stability and antigenicity. To establish a simple method to extract the protein antigens, two emulsified vaccines, designated as Cap-206 and Cap-35, were prepared by formulating a 28-kDa capsid protein (Cap) of PCV2b with ISA 206, a water-in-oil-in-water emulsion, or ISA 35, an oil-in-water emulsion. We found that the freeze-thaw-centrifugation method with steps of freezing at -20 °C for 12 h, thawing at room temperature and centrifuging at 9000×g for 10 min could separate the aqueous phase from Cap-206, and a centrifugation method by centrifuging at 9000×g for 10 min could isolate the aqueous portion from Cap-35...
February 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28189484/expression-purification-and-evaluation-of-recombinant-lipoprotein-of-salmonella-typhi-as-a-vaccine-candidate
#12
Anand Kumar, Subir Kundu, Mira Debnath Das
Lipoprotein has been reported as a vaccine candidate against many pathogenic bacteria, it plays direct role as a virulence-associated function. Here the approach is toward the expression of recombinant lipoprotein of Salmonella typhi in prokaryotic host and its evaluation as a vaccine candidate. Lipoprotein gene (lp1) was cloned in pET32a expression vector in addition to Bam HI and Hind III restriction sites, and BL21(pLysS) was used as prokaryotic expression host for transformation. Lipoprotein induction was performed by IPTG and 55 kDa (31 kDa of Gene +24 kDa of vector additional protein with His-tag) was analyzed by 12% SDS-PAGE...
February 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28189483/electrical-stimulation-of-somatic-human-stem-cells-mediated-by-composite-containing-conductive-nanofibers-for-ligament-regeneration
#13
Masumeh Dodel, Nahid Hemmati Nejad, S Hajir Bahrami, Masoud Soleimani, Leila Mohammadi Amirabad, Hana Hanaee-Ahvaz, Amir Atashi
One of the advances in the field of biomedical nanotechnology, is conductive nanofiber fabrication and the discovery of its applications. Biocompatible flexible nanofibers that have a good biocompatibility, mechanical properties and morphology. Poly (3, 4-ethylene dioxythiophene) (PEDOT) is a conductive polymer that has recently been used in medical applications. In this study, the electrospinning technique and vapor phase polymerization combination method with freeze drying was used to produce Silk fibroin/PEDOT/Chitosan nanocomposite scaffold...
February 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28173977/identification-of-a-candidate-standard-strain-of-severe-fever-with-thrombocytopenia-syndrome-virus-for-vaccine-quality-control-in-china-using-a-cross-neutralization-assay
#14
Zheng Jia, Xiaohong Wu, Ling Wang, Xiuling Li, Xinxian Dai, Mifang Liang, Shouchun Cao, Yan Kong, Jingjing Liu, Yuhua Li, Junzhi Wang
Severe fever with thrombocytopenia syndrome (SFTS) is caused by a phlebovirus of the Bunyaviridae family, which is designated as SFTS virus (SFTSV). To our knowledge, no efficient SFTSV vaccine exists. Here, we report the identification of a standard virus strain for the eight major SFTSV strains circulating in China for use in evaluating the SFTSV vaccine. Rabbits were immunized with the SFTSV strains and the cross-neutralization capacities of SFTSV anti-sera were determined in microculture cytopathic effect (CPE)-inhibition assays...
February 4, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28169066/standardization-and-validation-of-simple-pcr-duplex-pcr-and-rapd-in-comparison-to-blood-smear-examination-for-diagnosing-bovine-tropical-theileriosis
#15
Vikrant Sudan, Daya Shanker, Amit Jaiswal, Amit Singh, Vijay Pandey
Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle...
February 3, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28162840/establishment-of-a-reference-material-for-standardization-of-the-anti-complementary-activity-test-in-intravenous-immunoglobulin-products-used-in-japan-a-collaborative-study
#16
Kiyoko Nojima, Kazu Okuma, Masaki Ochiai, Madoka Kuramitsu, Kenta Tezuka, Mieko Ishii, Sadao Ueda, Takashi Miyamoto, Koichiro Kamimura, Enki Kou, Sanae Uchida, Yoshiharu Watanabe, Yoshiaki Okada, Isao Hamaguchi
Aggregates of human plasma-derived intravenous immunoglobulins (IVIGs) carries a risk of severe adverse events after nonspecific complement activation induced in humans administrated. Therefore, the anti-complementary activity (ACA) test is legally required in every batch of IVIGs in Japan. However, due to the intrinsic nature of this bioassay, there might be large differences in the results of ACA tests from laboratories, even when the same batch of IVIGs was measured. Our six laboratories evaluated whether there were such differences and argued for establishment of a reference material (RM) for standardization of the ACA test...
February 2, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28131552/comparative-analysis-of-the-feline-immunoglobulin-repertoire
#17
Sebastian C J Steiniger, Jacob Glanville, Douglas W Harris, Thomas L Wilson, Gregory C Ippolito, Steven A Dunham
Next-Generation Sequencing combined with bioinformatics is a powerful tool for analyzing the large number of DNA sequences present in the expressed antibody repertoire and these data sets can be used to advance a number of research areas including antibody discovery and engineering. The accurate measurement of the immune repertoire sequence composition, diversity and abundance is important for understanding the repertoire response in infections, vaccinations and cancer immunology and could also be useful for elucidating novel molecular targets...
January 25, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28122669/active-immunization-of-cattle-with-a-bothropic-toxoid-does-not-abrogate-envenomation-by-bothrops-asper-venom-but-increases-the-likelihood-of-survival
#18
María Herrera, Katherine González, Carlos Rodríguez, Aarón Gómez, Álvaro Segura, Mariángela Vargas, Mauren Villalta, Ricardo Estrada, Guillermo León
This study assessed the protective effect of active immunization of cattle to prevent the envenomation induced by B. asper venom. Two groups of oxen were immunized with a bothropic toxoid and challenged by an intramuscular injection of either 10 or 50 mg B. asper venom, to induce moderate or severe envenomations, respectively. Non-immunized oxen were used as controls. It was found that immunized oxen developed local edema similar to those observed in non-immunized animals. However, systemic effects were totally prevented in immunized oxen challenged with 10 mg venom, and therefore antivenom treatment was not required...
January 22, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28111083/a-competitive-elisa-for-detection-of-group-specific-antibody-to-bluetongue-virus-using-anti-core-antibody
#19
Karam Chand, Sanchay K Biswas, Awadh B Pandey, Arpit Saxena, Neha Tewari, Bimalendu Mondal
Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA...
January 19, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28108210/comparison-of-various-types-of-collagenous-scaffolds-applied-for-embryonic-nerve-cell-culture
#20
Jacek Drobnik, Krystyna Pietrucha, Marcin Kudzin, Krystyna Mader, Jacek Szymański, Alicja Szczepanowska
The purpose of the study was to confirm whether collagen-based scaffolds using different cross-linking methods are suitable elaborate environments for embryonic nerve cell culture. Three 3D sponge-shaped porous scaffolds were composed using collagen alone, collagen with chondroitin sulphate modified by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride, and collagen cross-linked by 2,3-dialdehyde cellulose (DAC). Embryonic nerve cells from rats were applied to the scaffolds and stained with bisbenzimide to study cell entrapment within the scaffolds...
January 17, 2017: Biologicals: Journal of the International Association of Biological Standardization
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