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Biologicals: Journal of the International Association of Biological Standardization

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https://www.readbyqxmd.com/read/27913028/active-immunization-with-brucella-abortus-s19-phage-lysate-elicits-serum-igg-that-protects-guinea-pigs-against-virulent-b-%C3%A2-abortus-and-protects-mice-by-passive-immunization
#1
Lata Jain, Mayank Rawat, Saravanan Ramakrishnan, Bablu Kumar
Brucellosis is an economically important zoonosis of worldwide significance. Earlier (Jain et al., 2015) we reported methodology for generation of phage lysate preparations against Brucella abortus S19 using brucellaphage 'ϕLd'. In this study, using a fixed dose (Two mouse PD100) of lysates, the prophylactic efficacies of both plain and alum gel adjuvanted lysates were evaluated in guinea pig by direct virulent challenge and passive mouse protection test (PMPT). Strong humoral and cell mediated immune responses in guinea pigs and protection comparable to S19 vaccine was observed with low dose (1...
November 29, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27876402/corrigendum-to-characterization-of-the-cell-growth-analysis-for-detection-of-immortal-cellular-impurities-in-human-mesenchymal-stem-cells-biologicals-43-2-march-2015-146-149
#2
Ken Kono, Nozomi Takada, Satoshi Yasuda, Rumi Sawada, Shingo Niimi, Akifumi Matsuyama, Yoji Sato
No abstract text is available yet for this article.
November 19, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27876270/a-new-manufacturing-process-to-remove-thrombogenic-factors-ii-vii-ix-x-and-xi-from-intravenous-immunoglobulin-gamma-preparations
#3
Dong Hwarn Park, Gil Bu Kang, Dae Eun Kang, Jeung Woon Hong, Min Gyu Lee, Ki Yong Kim, Jeung Whan Han
Coagulation factors (II, VII, IX, X, and particularly XIa) remaining in high concentrations in intravenous immunoglobulin (IVIG) preparations can form thrombi, causing thromboembolic events, and in serious cases, result in death. Therefore, manufacturers of biological products must investigate the ability of their production processes to remove procoagulant activities. Previously, we were able to remove coagulation factors II, VII, IX, and X from our IVIG preparation through ethanol precipitation, but factor XIa, which plays an important role in thrombosis, remained in the intermediate products...
November 18, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27865586/erythropoietin-induces-production-of-hepatocyte-growth-factor-from-bone-marrow-mesenchymal-stem-cells-in%C3%A2-vitro
#4
Kaveh Tari, Amir Atashi, Saied Kaviani, Mahshid AkhavanRahnama, Azadeh Anbarlou, Majid Mossahebi-Mohammadi
BACKGROUND: Hepatocyte Growth Factor (HGF) plays a pivotal role in hematopoiesis, motility, growth and mobilization of hematopoietic stem/progenitor cells (HSPCs). HGF mainly is produced by bone marrow mesenchymal stem cells (BM-MSCs). MSCs express erythropoietin (EPO) receptor. In this study, we aimed to assess the effect of EPO on HGF secretion in BM-MSCs. METHODS: The BM-MSCs treated with EPO (4 IU/ml) for 6, 24 and 48 h. HGF gene expression and protein level were assessed using quantitative real time PCR (qRT-PCR) and Enzyme-linked immunosorbant Assay...
November 16, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27843062/protection-of-mice-against-gastric-colonization-of-helicobacter-pylori-by-therapeutic-immunization-with-systemic-whole-cell-inactivated-vaccines
#5
K Suganya, A Prem Kumar, B Sekar, B Sundaran
The protective effect of therapeutic immunization with heat inactivated Helicobacter pylori cells administered with aluminum phosphate as an adjuvant was evaluated with "Swiss albino mice" infected with H. pylori Sydney strain 1 (SS1). The presence of bacteria in histological sections of the stomach was evaluated to confirm the colonization of H. pylori. The infection dose was determined to be 1 × 10(8) cells which resulted to be the optimal concentration to sustain infection for required time. Systemic immunization of H...
November 11, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27836582/antibodies-raised-against-divalent-type-b-flagellin-and-pilin-provide-effective-immunotherapy-against-pseudomonas-aeruginosa-infection-of-mice-with-burn-wounds
#6
Mahmood Saffari, Somayeh Behbood, Gholamreza Irajian, Ahmad Khorshidi, Rezvan Moniri, Bahador Behrouz
Burn wound infections caused by multidrug-resistant Pseudomonas aeruginosa strains are a serious challenge to therapy because of the complex pathogenesis and paucity of new effective antibiotics. Therefore, there is renewed interest in developing antibody-based therapeutic strategies. Immunotherapy strategies typically target selected virulence factors that are expressed by the majority of clinical strains of P. aeruginosa, particularly because virulence factors mediate infection. Here we used a murine model of burn wound infection to evaluate the efficacy of antibodies raised against the divalent type b flagellin and PilA (flagellin b + PilA), as acute virulence factors, to prevent and treat infection...
November 8, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27816388/a-comparison-of-methods-for-extracting-plasmids-from-a-difficult-to-lyse-bacterium-lactobacillus-casei
#7
Mojtaba Alimolaei, Mehdi Golchin
There are few practical protocols to extract efficient plasmid DNA from the difficult-to-lyse bacterium, Lactobacillus casei. This is related to production of a large amount of exopolysaccharide coat and its special physiological characteristics. In this study, we optimized a protocol to extract efficient plasmid DNA from a recombinant L. casei strain. Different extraction methods were evaluated in three classes of conventional, kit-based, and combined protocols. The quantity and quality of the extracted plasmid DNA were determined by spectrophotometry, agarose gel electrophoresis, and PCR...
November 2, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27814939/validation-of-high-throughput-screening-of-human-sera-for-detection-of-anti-pa-igg-by-enzyme-linked-immunosorbent-assay-elisa-as-an-emergency-response-to-an-anthrax-incident
#8
Vera A Semenova, Evelene Steward-Clark, Panagiotis Maniatis, Monica Epperson, Amit Sabnis, Jarad Schiffer
To improve surge testing capability for a response to a release of Bacillus anthracis, the CDC anti-Protective Antigen (PA) IgG Enzyme-Linked Immunosorbent Assay (ELISA) was re-designed into a high throughput screening format. The following assay performance parameters were evaluated: goodness of fit (measured as the mean reference standard r(2)), accuracy (measured as percent error), precision (measured as coefficient of variance (CV)), lower limit of detection (LLOD), lower limit of quantification (LLOQ), dilutional linearity, diagnostic sensitivity (DSN) and diagnostic specificity (DSP)...
November 1, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27810255/is-pichia-pastoris-a-realistic-platform-for-industrial-production-of-recombinant-human-interferon-gamma
#9
Ali Razaghi, Emilyn Tan, Linda H L Lua, Leigh Owens, O P Karthikeyan, Kirsten Heimann
Human interferon gamma (hIFNγ) is an important cytokine in the innate and adaptive immune system, produced commercially in Escherichia coli. Efficient expression of hIFNγ has been reported once for Pichia pastoris (Wang et al., 2014) - a proven heterologous expression system. This study investigated hIFNγ expression in P. pastoris replicating the previous study and expanding by using four different strains (X33: wild type; GS115: HIS(-)Mut(+); KM71H: Arg(+), Mut(-) and CBS7435: Mut(S)) and three different vectors (pPICZαA, pPIC9 and pPpT4αS)...
October 31, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27776829/comparative-study-of-profiling-post-translational-modifications-of-a-circulating-antibody-drug-in-human-with-different-capture-reagents
#10
Yue Zhang, Shiaw-Lin Wu, Yinyin Li
Capture reagents are critical to affinity-based bioanalytical methods. The potential bias of capture reagents, for or against certain subpopulations of the target of interest, may lead to inaccurate quantitation. This issue is more profound for sensitive measurements, such as post-translational modification (PTM) profiling of therapeutic proteins from complex matrix. Here, a recently developed affinity purification coupled mass spectrometric method was utilized to assess the full sequence of a circulating therapeutic aglycosylated IgG1 (MAB3) in human subject, using two different capture reagents...
October 21, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27773423/applicability-of-human-osteoarthritic-chondrocytes-for-in%C3%A2-vitro-efficacy-testing-of-anti-tnf%C3%AE-drugs
#11
Sara Žigon-Branc, Matjaž Jeras, Andrej Blejec, Ariana Barlič
In vitro cell-based models are important tools for assessing efficacies of new leads in early phases of drug development. Human osteoarthritic chondrocytes (OACs), obtained from biomedical waste material, represent a valuable, relatively accessible cellular source that could be used for this purpose. By employing reverse transcription-polymerase chain reaction (qRT-PCR) we compared gene expression profiles of key anabolic, catabolic and inflammatory genes of freshly isolated vs. monolayer cultured OACs (passages P0-P2) and non-stimulated vs...
October 20, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27756679/pre-registration-efficacy-study-of-a-novel-marker-vaccine-against-classical-swine-fever-on-maternally-derived-antibody-positive-mda-target-animals
#12
A Farsang, R Lévai, T Barna, K Fábián, S Blome, K Belák, Á Bálint, F Koenen, G Kulcsár
Maternally Derived Antibodies (MDA) can have a negative effect on the efficacy of live attenuated vaccines against classical swine fever (CSF). For this reason, a marker vaccine candidate CP7_E2alf was tested for its efficacy in the presence of MDA. Pregnant sows were vaccinated four weeks before farrowing with CSF virus (CSFV) strain "Thiverval". A total of 40 piglets with MDAs were included in this study. At six weeks of age the piglets were allocated into three treatment groups using generalized randomized block design (GRBD) blocking on serological status and pen location...
October 15, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27751695/improved-proliferation-and-osteogenic-differentiation-of-mesenchymal-stem-cells-on-polyaniline-composited-by-polyethersulfone-nanofibers
#13
Faeze Pournaqi, Armaghan Ghiaee, Saeid Vakilian, Abdolreza Ardeshirylajimi
Tissue engineering is a promising emerged method trying to reconstruct lost tissues that using synthetic and biomaterials and their combination with cells. The purpose of this study is increase osteoinductivity of polyethersulfone (PES) by using polyaniline (PANi). In this study, after fabrication of PES and composited PES-PANi scaffolds by electrospinning, scaffolds were characterized morphologically and mechanically. Then osteoinductivity of scaffolds was investigated by osteogenic differentiation of human mesenchymal stem cells (MSCs) cultured on the PES and PES-PANI in comparison to the tissue culture polystyrene as a control...
October 14, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27746073/characterization-of-duck-plague-virus-stability-at-extreme-conditions-of-temperature-ph-and-salt-concentration
#14
Aditi Makhija, Sachin Kumar
Duck plaque virus (DPV) belongs to the family Herpesviridae. The live attenuated vaccine is the only way to protect the ducks from DPV infection. The ineffectiveness of vaccine is one of the major causes of DPV outbreaks in the field condition. DPV is not well characterized for its pathogenicity and molecular biology in poultry. In the present study, we discuss briefly about the biology of DPV and its proteins under different conditions of temperature and pH in order to evaluate its infectivity under adverse physical conditions...
October 13, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27733310/study-of-twenty-preparations-of-human-albumin-solution-which-failed-in-quality-control-testing-due-to-elevated-sodium-content-a-poor-internal-quality-control-at-manufacturing-unit
#15
J P Prasad, Y Madhu, Surinder Singh, G R Soni, N Agnihotri, Varsha Singh, Pradeep Kumar, Nidhi Jain, Anu Prakash, Varun Singh
Current study is conducted in our laboratory due to failure in quality control testing of twenty batches of Human Albumin solution in which sodium content is higher than the prescribed limit. These batches are received in short duration from indigenous manufacturer and is the first incident of failure of Human albumin preparation in sodium content of manufacturer. On request of manufacturer, study is conducted to rule out the cause. Repeat testing of each out of specification batch is conducted and a trend analysis is drawn between our findings and manufacturer's results, also study of trend analysis of manufacturer for the last one year...
October 9, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27733309/construction-and-immunogenic-properties-of-a-chimeric-protein-comprising-cfae-cfab-and-ltb-against-enterotoxigenic-escherichia-coli
#16
Seyed-Mohammad Gheibi Hayat, Seyed-Latif Mousavi Gargari, Shahram Nazarian
ETEC (Enterotoxigenic Escherichia coli) is a major cause of diarrhea in developing countries and children. ETEC has two virulence factors including colonization factors antigen (CFA) and labile enterotoxins (LTs). CFA/I consists the major pilin subunit CfaB and a minor adhesive subunit, CfaE. In this study a tripartite fusion protein containing CfaB, CfaE and LTB was designed. In silico analysis of the tertiary structure of the chimeric protein showed a protein with three main domains linked together with linkers...
October 9, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27726931/rabies-vaccine-response-measurement-is-assay-dependent
#17
Susan M Moore, Samantha Pralle, Leslie Engelman, Hattie Hartschuh, Mylissia Smith
Vaccine equivalency, booster administration, and animal import decisions are based in part on the level of rabies virus neutralizing antibody (RVNA) in serum. Serum neutralization (SN) is commonly used but other methods are employed. Studies have shown that although enzyme-linked immunosorbent assay (ELISA) and SN results are correlated, exact comparison cannot be ensured. This study investigated the applicability of the recognized 0.5 IU/mL cut-off value between methods. Serum from rabies vaccinated subjects grouped by vaccine and vaccination regimen were collected on days 0, 14, 30, and 90 and tested by both SN and ELISA methods...
October 7, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27720268/development-of-inactivated-poliovirus-vaccine-from-sabin-strains-a%C3%A2-progress-report
#18
Hiromasa Okayasu, Carolyn Sein, Ahd Hamidi, Wilfried A M Bakker, Roland W Sutter
The Global Polio Eradication Initiative (GPEI) has seen significant progress since it began in 1988, largely due to the worldwide use of oral poliovirus vaccine (OPV). In order to achieve polio eradication the global cessation of OPV is necessary because OPV contains live attenuated poliovirus, which in rare circumstances could re-gain wild poliovirus (WPV) characteristics with potential to establish transmission. The GPEI endgame strategy for the period 2013-2018 recommends the globally synchronised sequential cessation of the Sabin strains contained in the OPV, starting with type 2 Sabin...
October 5, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27720267/enhancement-of-stem-cell-differentiation-to-osteogenic-lineage-on-hydroxyapatite-coated-hybrid-plga-gelatin-nanofiber-scaffolds
#19
Parisa Sanaei-Rad, Tahereh-Sadat Jafarzadeh Kashi, Ehsan Seyedjafari, Masoud Soleimani
A combination of polymeric materials and bioceramics has recently received a great deal of attention for bone tissue engineering applications. In the present study, hybrid nanofibrous scaffolds were fabricated from PLGA and gelatin via electrospinning and then were coated with hydroxyapatite (HA). They were then characterized and used in stem cell culture studies for the evaluation of their biological behavior and osteogenic differentiation in vitro. This study showed that all PLGA, hybrid PLGA/gelatin and HA-PLGA/gelatin scaffolds were composed of ultrafine fibers with smooth morphology and interconnected pores...
October 5, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27712979/novel-polyacrylate-based-cationic-nanoparticles-for-survivin-sirna-delivery-combined-with-mitoxantrone-for-treatment-of-breast-cancer
#20
Sanam Arami, Majid Mahdavi, Mohammad Reza Rashidi, Marziyeh Fathi, Mohammad-Saeid Hejazi, Nasser Samadi
As a gene delivery method in breast cancer therapy, knocking down the undesired genes in the cancerous cells would be promising. Inhibitors of Apoptosis Protein (IAP) family genes are some of the genes whose responsibility is inhibition of apoptosis in cells. Silencing these genes seems to be helpful directing the tumor cells to death. siRNA sequence designed against survivin anti-apoptotic gene can play this role if carried to the cytoplasm. Here we prepared a positive charged biocompatible nano-sized particle made up of a Fe3O4 core covered respectively by polyacrylate (PA) and polyethyleneimine (PEI) layer, which could successfully deliver the siRNA into the MCF-7 cells...
October 3, 2016: Biologicals: Journal of the International Association of Biological Standardization
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