journal
MENU ▼
Read by QxMD icon Read
search

Biologicals: Journal of the International Association of Biological Standardization

journal
https://www.readbyqxmd.com/read/28100412/virome-of-us-bovine-calf-serum
#1
Mohammadreza Sadeghi, Beatrix Kapusinszky, Danielle M Yugo, Tung Gia Phan, Xutao Deng, Isis Kanevsky, Tanja Opriessnig, Amelia R Woolums, David J Hurley, Xiang-Jin Meng, Eric Delwart
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses...
January 15, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28087107/establishment-of-the-2nd-korean-national-biological-reference-standard-for-blood-coagulation-factor-viii-c-concentrate
#2
Naery Lee, Ji Suk Seo, Jae Ok Kim, Sang Ja Ban
Since the 1st Korean national biological reference standard for factor (F)VIII concentrate, established in 2001, has shown declining potency, we conducted this study to replace this standard with a 2nd Korean national biological reference standard for blood coagulation FVIII concentrate. The candidate materials for the 2nd standard were prepared in 8000 vials with 10 IU/ml of target potency, according to the approved manufacturing process of blood coagulation Factor VIII:C Monoclonal Antibody-purified, Freeze-dried Human Blood Coagulation Factor VIII:C...
January 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28087106/charge-variant-analysis-of-proposed-biosimilar-to-trastuzumab
#3
Pravinkumar Dakshinamurthy, Pavithra Mukunda, Bhargav Prasad Kodaganti, Bharath Ravindra Shenoy, Bairavabalakumar Natarajan, Amol Maliwalave, Vivek Halan, Sathyabalan Murugesan, Sunit Maity
Trastuzumab is a humanized monoclonal antibody (mAb) employed for the treatment of HER2 Positive Breast Cancer. A HER2 overexpressing tumor cell binds to Trastuzumab and attracts immune cells which lead to induction of Antibody Dependent Cellular Cytotoxicity (ADCC) by binding to Fc receptors (CD16a or FcγRIIIa) on an effector cell, such as natural killer (NK) cells. The most commonly expressed receptor on NK cell is CD16a which binds to the Fc portion of Trastuzumab. The ligand-independent HER2-HER3 dimerization is the most potent stimulator of downstream pathways for regulation of cell growth and survival...
January 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28082100/a-collaborative-study-to-establish-the-3rd-who-international-standard-for-hepatitis-b-virus-for-nucleic-acid-amplification-techniques
#4
Jacqueline F Fryer, Alan B Heath, Dianna E Wilkinson, Philip D Minor
Nucleic acid amplification techniques (NAT) are routinely used for clinical diagnostics and monitoring hepatitis B virus (HBV) infections, and are implemented on a voluntary basis for blood screening. A collaborative study was performed to evaluate a replacement WHO International Standard for HBV for the standardization of NAT. Two lyophilised HBV candidates were evaluated by 16 laboratories worldwide, alongside the existing HBV International Standard. The overall mean potency estimates for the candidate samples 1 and 2, relative to sample 3 (2nd HBV International Standard), from quantitative assays, were 5...
January 9, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28065582/bivalent-flagellin-immunotherapy-protects-mice-against-pseudomonas-aeruginosa-infections-in-both-acute-pneumonia-and-burn-wound-models
#5
Hosein Ahmadi, Bahador Behrouz, Gholamreza Irajian, Nour Amirmozafari, Sara Naghavi
Pseudomonas aeruginosa infections are a serious challenge to therapy because of the complex pathogenesis and paucity of new effective antibiotics, thus renewing interest in antibody-based therapeutic strategies. Immunotherapy strategies typically target selected virulence factors that are expressed by the majority of clinical strains of P. aeruginosa, particularly because virulence factors mediate infection. Type a and b flagellins (flagellin a+b) of P. aeruginosa are acute virulence factors that play a major role in the establishment of infection...
January 5, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28040390/reduction-of-animal-suffering-in-rabies-vaccine-potency-testing-by-introduction-of-humane-endpoints
#6
Mutsuyo Takayama-Ito, Chang-Kweng Lim, Kazuo Nakamichi, Satsuki Kakiuchi, Madoka Horiya, Guillermo Posadas-Herrera, Ichiro Kurane, Masayuki Saijo
Potency controls of inactivated rabies vaccines for human use are confirmed by the National Institutes of Health challenge test in which lethal infection with severe neurological symptoms should be observed in approximately half of the mice inoculated with the rabies virus. Weight loss, decreased body temperature, and the presence of rabies-associated neurological signs have been proposed as humane endpoints. The potential for reduction of animal suffering by introducing humane endpoints in the potency test for inactivated rabies vaccine for human use was investigated...
December 28, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28027847/improvement-influenza-ha2-dna-vaccine-cellular-and-humoral-immune-responses-with-mx-bio-adjuvant
#7
Sina Soleimani, Shahla Shahsavandi, Omid Maddadgar
Immunization with DNA vaccines as a novel alternative to conventional vaccination strategy requires adjuvant for improving vaccine efficacy. The conserved immunogenic HA2 subunit, which harbors neutralizing epitopes is a promising vaccine candidate against influenza viruses. In this study, for the first time we explore the idea of using host interferon inducible Mx protein to increase the immunogenicity of HA2 H9N2 influenza DNA vaccine. The potency and safety of the Mx adjuvanted-HA2 vaccine was evaluated in BALB/c mice by different prime-boost strategies...
December 24, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28017506/insulin-producing-cells-generation-by-overexpression-of-mir-375-in-adipose-derived-mesenchymal-stem-cells-from-diabetic-patients
#8
Mehran Piran, Seyed Ehsan Enderami, Mehrdad Piran, Hadis Soltani Sedeh, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi
Diabetes Mellitus (DM) is a systematic disease, which happens because of destruction of islets of Langerhans in the pancreas and systematic resistance to insulin. The lack of donor for pancreas transplantation and risk of transplant rejection is the main challenge in the treatment of this disease. Stem cells are proper and sufficient source for creating insulin-producing cells (IPC). In this study adipose tissue was provided from diabetic patients operated for liposuction and then adipose derived stem cells (ADSCs) were isolated, characterized and then treated by lentiviruses containing miR-375, after 7, 14 and 21 days of induction, islet-like clusters (ILC) specific genes including insulin and PDX1 were evaluated by Real Time RT-PCR...
December 22, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28012703/physicochemical-characterization-and-immunological-properties-of-pichia-pastoris-based-hpv16l1-and-18l1-virus-like-particles
#9
Gaurav Gupta, Reinhard Glueck, Narayan Rishi
There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels...
December 21, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27923539/the-roles-of-the-co-culture-of-mescs-with-pancreatic-islets-and-liver-stromal-cells-in-the-differentiation-of-definitive-endoderm-cells
#10
Hoveizi Elham, Fathi Fardin, Hashemitabar Mahmod
Embryonic stem (ES) cells have a pluripotent ability to differentiate into a variety of cell lineages in vitro. Investigating the roles of the co-culture of mouse embryonic stem cells (mESCs) with pancreatic islets (PL) and liver stromal cells (LSCs) in the differentiation of definitive endoderm (DE) cells was the purpose of this study. Here by using PL derived from adult mouse and LSCs derived from mouse fetal liver, we are trying to introduce a new protocol which is devoid of growth factors. We calculated mESCs indirectly for 7 days and then we analyzed the resulting cells regarding DE genes and protein expression using qRT-PCR and immunocytochemistry...
December 3, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27913028/active-immunization-with-brucella-abortus-s19-phage-lysate-elicits-serum-igg-that-protects-guinea-pigs-against-virulent-b-%C3%A2-abortus-and-protects-mice-by-passive-immunization
#11
Lata Jain, Mayank Rawat, Saravanan Ramakrishnan, Bablu Kumar
Brucellosis is an economically important zoonosis of worldwide significance. Earlier (Jain et al., 2015) we reported methodology for generation of phage lysate preparations against Brucella abortus S19 using brucellaphage 'ϕLd'. In this study, using a fixed dose (Two mouse PD100) of lysates, the prophylactic efficacies of both plain and alum gel adjuvanted lysates were evaluated in guinea pig by direct virulent challenge and passive mouse protection test (PMPT). Strong humoral and cell mediated immune responses in guinea pigs and protection comparable to S19 vaccine was observed with low dose (1...
November 29, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27876402/corrigendum-to-characterization-of-the-cell-growth-analysis-for-detection-of-immortal-cellular-impurities-in-human-mesenchymal-stem-cells-biologicals-43-2-march-2015-146-149
#12
Ken Kono, Nozomi Takada, Satoshi Yasuda, Rumi Sawada, Shingo Niimi, Akifumi Matsuyama, Yoji Sato
No abstract text is available yet for this article.
November 19, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27876270/a-new-manufacturing-process-to-remove-thrombogenic-factors-ii-vii-ix-x-and-xi-from-intravenous-immunoglobulin-gamma-preparations
#13
Dong Hwarn Park, Gil Bu Kang, Dae Eun Kang, Jeung Woon Hong, Min Gyu Lee, Ki Yong Kim, Jeung Whan Han
Coagulation factors (II, VII, IX, X, and particularly XIa) remaining in high concentrations in intravenous immunoglobulin (IVIG) preparations can form thrombi, causing thromboembolic events, and in serious cases, result in death. Therefore, manufacturers of biological products must investigate the ability of their production processes to remove procoagulant activities. Previously, we were able to remove coagulation factors II, VII, IX, and X from our IVIG preparation through ethanol precipitation, but factor XIa, which plays an important role in thrombosis, remained in the intermediate products...
November 18, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27865586/erythropoietin-induces-production-of-hepatocyte-growth-factor-from-bone-marrow-mesenchymal-stem-cells-in%C3%A2-vitro
#14
Kaveh Tari, Amir Atashi, Saied Kaviani, Mahshid AkhavanRahnama, Azadeh Anbarlou, Majid Mossahebi-Mohammadi
BACKGROUND: Hepatocyte Growth Factor (HGF) plays a pivotal role in hematopoiesis, motility, growth and mobilization of hematopoietic stem/progenitor cells (HSPCs). HGF mainly is produced by bone marrow mesenchymal stem cells (BM-MSCs). MSCs express erythropoietin (EPO) receptor. In this study, we aimed to assess the effect of EPO on HGF secretion in BM-MSCs. METHODS: The BM-MSCs treated with EPO (4 IU/ml) for 6, 24 and 48 h. HGF gene expression and protein level were assessed using quantitative real time PCR (qRT-PCR) and Enzyme-linked immunosorbant Assay...
November 16, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27843062/protection-of-mice-against-gastric-colonization-of-helicobacter-pylori-by-therapeutic-immunization-with-systemic-whole-cell-inactivated-vaccines
#15
K Suganya, A Prem Kumar, B Sekar, B Sundaran
The protective effect of therapeutic immunization with heat inactivated Helicobacter pylori cells administered with aluminum phosphate as an adjuvant was evaluated with "Swiss albino mice" infected with H. pylori Sydney strain 1 (SS1). The presence of bacteria in histological sections of the stomach was evaluated to confirm the colonization of H. pylori. The infection dose was determined to be 1 × 10(8) cells which resulted to be the optimal concentration to sustain infection for required time. Systemic immunization of H...
November 11, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27836582/antibodies-raised-against-divalent-type-b-flagellin-and-pilin-provide-effective-immunotherapy-against-pseudomonas-aeruginosa-infection-of-mice-with-burn-wounds
#16
Mahmood Saffari, Somayeh Behbood, Gholamreza Irajian, Ahmad Khorshidi, Rezvan Moniri, Bahador Behrouz
Burn wound infections caused by multidrug-resistant Pseudomonas aeruginosa strains are a serious challenge to therapy because of the complex pathogenesis and paucity of new effective antibiotics. Therefore, there is renewed interest in developing antibody-based therapeutic strategies. Immunotherapy strategies typically target selected virulence factors that are expressed by the majority of clinical strains of P. aeruginosa, particularly because virulence factors mediate infection. Here we used a murine model of burn wound infection to evaluate the efficacy of antibodies raised against the divalent type b flagellin and PilA (flagellin b + PilA), as acute virulence factors, to prevent and treat infection...
November 8, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27816388/a-comparison-of-methods-for-extracting-plasmids-from-a-difficult-to-lyse-bacterium-lactobacillus-casei
#17
Mojtaba Alimolaei, Mehdi Golchin
There are few practical protocols to extract efficient plasmid DNA from the difficult-to-lyse bacterium, Lactobacillus casei. This is related to production of a large amount of exopolysaccharide coat and its special physiological characteristics. In this study, we optimized a protocol to extract efficient plasmid DNA from a recombinant L. casei strain. Different extraction methods were evaluated in three classes of conventional, kit-based, and combined protocols. The quantity and quality of the extracted plasmid DNA were determined by spectrophotometry, agarose gel electrophoresis, and PCR...
November 2, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27814939/validation-of-high-throughput-screening-of-human-sera-for-detection-of-anti-pa-igg-by-enzyme-linked-immunosorbent-assay-elisa-as-an-emergency-response-to-an-anthrax-incident
#18
Vera A Semenova, Evelene Steward-Clark, Panagiotis Maniatis, Monica Epperson, Amit Sabnis, Jarad Schiffer
To improve surge testing capability for a response to a release of Bacillus anthracis, the CDC anti-Protective Antigen (PA) IgG Enzyme-Linked Immunosorbent Assay (ELISA) was re-designed into a high throughput screening format. The following assay performance parameters were evaluated: goodness of fit (measured as the mean reference standard r(2)), accuracy (measured as percent error), precision (measured as coefficient of variance (CV)), lower limit of detection (LLOD), lower limit of quantification (LLOQ), dilutional linearity, diagnostic sensitivity (DSN) and diagnostic specificity (DSP)...
November 1, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27810255/is-pichia-pastoris-a-realistic-platform-for-industrial-production-of-recombinant-human-interferon-gamma
#19
Ali Razaghi, Emilyn Tan, Linda H L Lua, Leigh Owens, O P Karthikeyan, Kirsten Heimann
Human interferon gamma (hIFNγ) is an important cytokine in the innate and adaptive immune system, produced commercially in Escherichia coli. Efficient expression of hIFNγ has been reported once for Pichia pastoris (Wang et al., 2014) - a proven heterologous expression system. This study investigated hIFNγ expression in P. pastoris replicating the previous study and expanding by using four different strains (X33: wild type; GS115: HIS(-)Mut(+); KM71H: Arg(+), Mut(-) and CBS7435: Mut(S)) and three different vectors (pPICZαA, pPIC9 and pPpT4αS)...
October 31, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27733310/study-of-twenty-preparations-of-human-albumin-solution-which-failed-in-quality-control-testing-due-to-elevated-sodium-content-a-poor-internal-quality-control-at-manufacturing-unit
#20
J P Prasad, Y Madhu, Surinder Singh, G R Soni, N Agnihotri, Varsha Singh, Pradeep Kumar, Nidhi Jain, Anu Prakash, Varun Singh
Current study is conducted in our laboratory due to failure in quality control testing of twenty batches of Human Albumin solution in which sodium content is higher than the prescribed limit. These batches are received in short duration from indigenous manufacturer and is the first incident of failure of Human albumin preparation in sodium content of manufacturer. On request of manufacturer, study is conducted to rule out the cause. Repeat testing of each out of specification batch is conducted and a trend analysis is drawn between our findings and manufacturer's results, also study of trend analysis of manufacturer for the last one year...
November 2016: Biologicals: Journal of the International Association of Biological Standardization
journal
journal
30393
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"