Yajun Shuai, Na Li, Ying Zhang, Qing Bao, Tiancheng Wei, Tao Yang, Qichao Cheng, Wei Wang, Baolan Hu, Chuanbin Mao, Mingying Yang
The detection of antibiotics is crucial for safeguarding the environment, ensuring food safety, and promoting human health. However, developing a rapid, convenient, low-cost, and sensitive method for antibiotic detection presents significant challenges. Herein, an aptamer-free biosensor was successfully constructed using upconversion nanoparticles (UCNPs) coated with silk fibroin (SF), based on Förster resonance energy transfer (FRET) and the charge-transfer effect, for detecting roxithromycin (RXM). A synergistic FRET efficiency was achieved by utilizing alizarin red and RXM complexes as energy acceptors, with UCNP as the energy donor, and immobilizing an ultrathin SF protein corona within 10 nm...
April 25, 2024: Biosensors & Bioelectronics